MD17G1045500.v1.1

Belongs to the AAA ATPase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0000219
Physical Location & Seq
Forward (+)
3280995 .. 3286094
5100 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1045500.v1.1.491

Sequence Viewer

Length: 2958 bp
ATGATGGGAGAAATGTGGGCTCAAACGGGCTCGGTGATTGCGAGCGTGATGTTCGTTGCTGCCATATTCCAACAATACCTTCCTCGCCAACTTCGGTACCATGTCGAAAGATACACTCAAAAATTAGTGAGCTTTGTCTACCCTTACATCCAAATCTCTTTTGATGAATTCACCAATGATTTTCGCAAGCGAAGTGAAATCTATTCCGCCATCCAGAGCTATCTCAGCGCGAAGTCTTCCACCCGGGCTAAGCGGTTAAAAGCTCATGATGTCAAGGACAGCAAGTCTGTAGTCCTTGGCATGGATGACAGTGAAGAAGTGACTGATGAATTTCAAGGCATTAATCTTTCATGGGCTTCGAGGAAACGAACGAAGAATCAGACTTCATTTTCTTTCTATCCTCAGGCTGATGAGAAGAATCATTATACGCTGACGTTCCACAGACGCCATCGAGATATCATCACGGGGTCTTACCTTGATTATGTGATAAAAGAAGGGAAGGCGATATCGGTGAGCAACAGGCAACGAAGGCTCTATATCAACAATCCTACACAGAATTGGTACTCCTACAGGGGGACAAAGTGGAGCCATGTAGTGTTTGAGCACCCGGCATCATTTGAAACCCTAGCAATGGACCCGAAAAAGAAGGAGGAAATCGTCAATGACCTCATCATGTTCAGAAAGGGAAAGGAGTACTATGCAAAAATCGGGAAGCCTTGGAAGCGGGGTTATCTACTTTACGGGCCACCAGGCACTGGAAAGTCTACCATGGTTGCTGCCATGTCTAATCTCATGGATTATGATGTCTATGATCTTGAATTAACGACGGTGAAGGACAACACTGAGCTGAGGAAGCTACTGATTGACACACCGAGTAAGTCTATACTTGTGATTGAGGACATTGATTGCTCGCTTGATCTTACAGGACAGCGAAAGAAGAAGAAGGAGAAGGATGAGGAGGACAAGGAAGAAAATAAGGATCCAGTTCGAAAAATGAGGGAAGATCAAGAAAACACACAGAGCAAGGTCACTCTTTCGGGGCTGCTAAACTTTATCGATGGGATTTGGTCAGCTTGCGGAGGGGAGAGACTGATCGTGTTCACTACTAATTATGTGGAGAAACTTGATCCTGCGCTCATTAGAAGAGGAAGGATGGACAAACACATAGAGTTGTCCTACTGCTGCTTCGAAGCATTCAAAGTGCTTGCTAGGAATTATTTGGATTTGGATTCACAAGAGCTGTTTGAAACGATTGCTCGTTTGTTGGGCGAAACCAATATGACTCCTGCTGATGTCGCTGAGAACTTGATGCCTAAGTCAGTTATACCCGATTCTGAATCTTGTTTGAAGAACTTGATCGAAGCTCTTGAGGCTGCGAAGGAGGAGGCAAGAGTGAAGGCCGAGGAAGAAGCAAAATCAAAGGCAGAGGAAGAGGCAAAACTGAAGGCAGTGGAAGCGGCAAAACTGAAGGAGATCCGAGAAAGTATACAAGAAAAAATGCGTATGATGGGAGAAATGTGGGCAAAATTAGGCTCGATGGTTGCGAGTGTGATGTTCATGAGTGCCGTATTTCGAGACTTCCTTCCGGTGCAACTTCGAAATCATGTCGAACTATACATCCATAAACTAGCGAACTTTTTCGACCCTTACGTGCAAATTACATTTGATGAGCACACCAATCAGTTTCGCAAACAAAGTGAAGTCTATTCCGCCATCCAGAGCTACCTCAGCACCAAGTTTTCTGCCCGAGTGAGGCGGCTCAAGGCTCATGATGTCAAGAACAGCAAGTCTCTAGTCCTTAGCATGGATGACAACGAAGAAATCACCGATGAATTTCAAGGCATTACGTTTTCGTGGGCTTCGGTGAAACGAATGGCGAACCAGACTTCATTTTCTATCTACCCTCAATCTAATGAGAAGAAATGTTATAAGCTGACGTTCCACAGACGCTATCGAGATATCATGACGGGGTCTTACCTTGATCATGTGATAAAAGAGGGGAAGGCGATATCGGCAAGCAACAGGCAAAGAAAGCTTTACATCAACAATCCTGCAGAGGATTGGAATTCATACCGAGGGACGAAATGGAGCCACATTGTTTTTGAGCACCCGGCAACATTTGAAAGCCTAGCAATGGACCCGAAAAAGAAGGAAGAAATCATCAATGACCTCATCAAGTTCAGTAAGGGAAAAGAGTACTATGCAAAAATCGGGAAGGCATGGAAGCGGGGTTATCTCCTCTACGGGCCACCAGGAACTGGAAAGTCTACCATGATTGCTGCCATTTCTAACCTCATGAACTACGATGTCTATGATCTTGAACTAACGCAGGTTAAGGACAACACTGAGCTGAGGAAGCTTCTGATAGACACGCCGAGTAAGTCTATAATTGTGATTGAGGACATTGATTGCTCGCTTGATCTTACCGGACAGCGAAAGAAGAAAAAGGATGAGGAGGACAAGGAAGAAAAGGATTCAGTTCGGAAAAAGAGGGAAGATCAAGAAAACAAACCGAGCAAGGTGACTCTTTCGGGGCTGCTAAACGTTATCGATGGGATTTGGTCAGCTTGTGGAGGGGAGAGACTGATCGTGTTTACGACTAATTATGTGGACAAGCTCGATCCTGCACTCATCAGAAGAGGAAGGATGGACAAGCACATAGAATTGTCCTACTGCTGCTTTGAAGCATTCAAAGTTCTTGCTAGGAATTATTTGGGTTTGGAGACGCACGAATTGTTTGAAAGGATTGGGCGTTTGTTGGGGGAAACGGATATGACACCTGCTGATGTTGCAGAGAATTTGATGCCGAAATCTGATGTAGAGGATGCTGAGTCTTGTTTGAAGAACTTGATCGAAGCTCTCGAGGCTGCGAAGGAGGAGGCAAAGATCAAGGCCGAGGAAGAAGCTCAACAGAAGGCAGAGAAAGACGAAAATTTGAATGCAGAGAAAGAAGCATAA

Protein Analysis

986

Amino Acids

113.7

Weight (kDa)

6.85

Isoelectric Point (pI)

36.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_assoc PF14363 27 - 120 1e-24 Domain associated at C-terminal with AAA
AAA PF00004 244 - 392 1.2e-16 ATPase family associated with various cellular activities (AAA)
AAA_lid_At3g28540 PF25568 394 - 467 5.8e-31 At3g28540-like, AAA+ ATPase lid domain
AAA_assoc PF14363 528 - 622 7.1e-25 Domain associated at C-terminal with AAA
AAA PF00004 745 - 890 3.3e-18 ATPase family associated with various cellular activities (AAA)
AAA_lid_At3g28540 PF25568 892 - 965 9.8e-34 At3g28540-like, AAA+ ATPase lid domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000219)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G28580 AT3G28580 AT5G40010
fragaria_vesca FvH4_5g15870 FvH4_6g49542 FvH4_6g49550 FvH4_6g49560 FvH4_6g49580
malus_domestica MD09G1043700.v1.1 MD09G1043800.v1.1 MD09G1043900.v1.1 MD14G1190900.v1.1 MD17G1045300.v1.1 MD17G1045500.v1.1 MD17G1045600.v1.1
prunus_persica Prupe.3G273800_v2.0.a1 Prupe.3G273900_v2.0.a1 Prupe.3G274100_v2.0.a1 Prupe.3G274200_v2.0.a1 Prupe.3G274300_v2.0.a1 Prupe.5G183800_v2.0.a1
pyrus_communis pycom111g03400 pycom111g03410 pycom14g15860 pycom17g04010 pycom17g04040 pycom17g04070 pycom17g04080
rosa_chinensis RchiOBHm_Chr2g0169521 RchiOBHm_Chr2g0169541 RchiOBHm_Chr2g0169551 RchiOBHm_Chr2g0169571 RchiOBHm_Chr2g0169581 RchiOBHm_Chr2g0169591 RchiOBHm_Chr2g0170131 RchiOBHm_Chr7g0180181
rosa_laevigata RLG00000005284 RLG00000021869 RLG00000021870 RLG00000021871 RLG00000021872 RLG00000021873 RLG00000021878 RLG00000021879 RLG00000021880 RLG00000021881 RLG00000021882 RLG00000021883 RLG00000021884 RLG00000021885 RLG00000021886 RLG00000021926 RLG00000021927
rosa_multiflora Rmu_co8112588.1_g000001 Rmu_co8118398.1_g000001 Rmu_co8187516.1_g000001 Rmu_co8476833.1_g000001 Rmu_sc0006806.1_g000003 Rmu_sc0006806.1_g000004 Rmu_sc0006806.1_g000005 Rmu_sc0006806.1_g000007 Rmu_sc0006806.1_g000008 Rmu_sc0006806.1_g000009 Rmu_sc0006806.1_g000010 Rmu_sc0006806.1_g000011 Rmu_sc0006806.1_g000012 Rmu_sc0006806.1_g000013 Rmu_sc0006806.1_g000026 Rmu_sc0006806.1_g000027 Rmu_sc0011963.1_g000001 Rmu_sc0013078.1_g000004 Rmu_sc0019635.1_g000002 Rmu_sc0019635.1_g000003 Rmu_sc0019635.1_g000004 Rmu_sc0019635.1_g000005 Rmu_sc0019635.1_g000006 Rmu_sc0019635.1_g000007 Rmu_sc0019635.1_g000008 Rmu_sc0019635.1_g000009
rosa_roxburghii Rroxscaffold_2G00081990 Rroxscaffold_2G00082000 Rroxscaffold_2G00082010 Rroxscaffold_2G00082020 Rroxscaffold_2G00082030 Rroxscaffold_2G00082040 Rroxscaffold_2G00082050 Rroxscaffold_3G00273050
rosa_rugosa Rorug02G0540500 Rorug02G0540600 Rorug02G0540700 Rorug02G0540800 Rorug02G0540900 Rorug02G0541000 Rorug02G0541100 Rorug02G0541100 Rorug02G0541200 Rorug02G0541300 Rorug02G0546000.1 Rorug06G0431600
rosa_samantha Rh2AG611900 Rh2AG612000 Rh2AG612100 Rh2AG612200 Rh2AG613000 Rh2AG613100 Rh2AG613200 Rh2AG613300 Rh2AG613400 Rh2AG613500 Rh2AG613600 Rh2AG613700 Rh2AG618200 Rh2BG625700 Rh2BG625800 Rh2BG625900 Rh2CG593800 Rh2CG593900 Rh2CG594300 Rh2CG594400 Rh2CG594500 Rh2CG594600 Rh2CG594800 Rh2CG594900 Rh2CG595000 Rh2CG595100 Rh2CG599000 Rh2DG636100 Rh2DG636200 Rh2DG636300 Rh2DG636400 Rh2DG636500 Rh2DG636600 Rh2DG636700 Rh2DG636800 Rh2DG636900 Rh2DG641400 Rh7AG031800 Rh7BG031800
rosa_wichuraiana Rw2G050750 Rw2G050790 Rw2G050820 Rw2G050830 Rw2G050840 Rw2G050850 Rw2G050860 Rw2G051180 Rw7G002650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1929
AarI CACCTGC 1 cut(s) 2788
Acc36I ACCTGC 2 cut(s) 2320, 2788
Acc65I GGTACC 1 cut(s) 96
AccB1I GGYRCC 1 cut(s) 96
AccB7I CCANNNNNTGG 2 cut(s) 755, 2258
AccI GTMKAC 4 cut(s) 138, 764, 1486, 2267
AccII CGCG 1 cut(s) 230
AciI CCGC 8 cut(s) 207, 253, 724, 1077, 1457, 1710, 1756, 2227
AclI AACGTT 1 cut(s) 2544
AclWI GGATC 5 cut(s) 974, 987, 1121, 1468, 2615
AcsI RAATTY 6 cut(s) 167, 329, 1832, 2065, 2797, 2932
AcuI CTGAAG 2 cut(s) 1463, 1487
AcyI GRCGYC 1 cut(s) 445
AfaI GTAC 4 cut(s) 98, 563, 695, 2198
AfiI CCNNNNNNNGG 8 cut(s) 301, 573, 631, 755, 1752, 1804, 2134, 2258
AhdI GACNNNNNGTC 1 cut(s) 283
AjnI CCWGG 2 cut(s) 748, 2251
AjuI GAANNNNNNNTTGG 2 cut(s) 63, 95
AloI GAACNNNNNNTCC 2 cut(s) 1602, 1634
Alw21I GWGCWC 3 cut(s) 606, 1674, 2109
Alw26I GTCTC 5 cut(s) 1081, 1569, 1794, 2575, 2717
AlwI GGATC 5 cut(s) 974, 987, 1121, 1468, 2615
AlwNI CAGNNNCTG 2 cut(s) 755, 2258
Ama87I CYCGRG 3 cut(s) 243, 1746, 2861
AoxI GGCC 4 cut(s) 743, 1398, 2246, 2892
ApeKI GCWGC 9 cut(s) 59, 776, 1042, 1182, 1373, 2279, 2536, 2676, 2867
ApoI RAATTY 6 cut(s) 167, 329, 1832, 2065, 2797, 2932
AseI ATTAAT 1 cut(s) 342
Asp700I GAANNNNTTC 1 cut(s) 1637
Asp718I GGTACC 1 cut(s) 96
AspLEI GCGC 2 cut(s) 230, 1135
AspS9I GGNCC 4 cut(s) 634, 743, 2137, 2246
AsuC2I CCSGG 4 cut(s) 244, 245, 608, 2111
AsuHPI GGTGA 7 cut(s) 46, 163, 523, 841, 1816, 1876, 2533
AsuII TTCGAA 3 cut(s) 988, 1188, 1597
AvaI CYCGRG 3 cut(s) 243, 1746, 2861
AvaII GGWCC 2 cut(s) 634, 2137
AxyI CCTNAGG 1 cut(s) 402
BaeI ACNNNNGTAYC 2 cut(s) 80, 113
BamHI GGATCC 1 cut(s) 979
BanI GGYRCC 1 cut(s) 96
BanII GRGCYC 2 cut(s) 22, 32
BbsI GAAGAC 1 cut(s) 228
Bbv12I GWGCWC 3 cut(s) 606, 1674, 2109
BbvCI CCTCAGC 3 cut(s) 848, 1727, 2351
BbvI GCAGC 9 cut(s) 46, 763, 1029, 1169, 1360, 2266, 2523, 2663, 2854
BccI CCATC 9 cut(s) 218, 456, 1052, 1147, 1501, 1531, 1721, 2546, 2641
BceAI ACGGC 1 cut(s) 1550
BciT130I CCWGG 2 cut(s) 750, 2253
BclI TGATCA 1 cut(s) 1981
BcnI CCSGG 4 cut(s) 244, 245, 608, 2111
BcoDI GTCTC 5 cut(s) 1081, 1569, 1794, 2575, 2717
BfaI CTAG 6 cut(s) 626, 1209, 1628, 1793, 2129, 2703
BfmI CTRYAG 3 cut(s) 288, 568, 2052
BfuAI ACCTGC 2 cut(s) 2320, 2788
BlpI GCTNAGC 1 cut(s) 249
BmcAI AGTACT 2 cut(s) 695, 2198
Bme1390I CCNGG 6 cut(s) 244, 245, 608, 750, 2111, 2253
Bme18I GGWCC 2 cut(s) 634, 2137
BmeRI GACNNNNNGTC 1 cut(s) 283
BmeT110I CYCGRG 3 cut(s) 243, 1746, 2861
BmgT120I GGNCC 4 cut(s) 634, 743, 2137, 2246
BmiI GGNNCC 6 cut(s) 98, 587, 636, 981, 2090, 2139
BmrFI CCNGG 6 cut(s) 244, 245, 608, 750, 2111, 2253
BmsI GCATC 4 cut(s) 620, 1299, 2793, 2815
BpiI GAAGAC 1 cut(s) 228
Bpu10I CCTNAGC 4 cut(s) 848, 1727, 1799, 2351
Bpu1102I GCTNAGC 1 cut(s) 249
Bpu14I TTCGAA 3 cut(s) 988, 1188, 1597
BpuEI CTTGAG 2 cut(s) 1388, 1745
BpuMI CCSGG 4 cut(s) 244, 245, 608, 2111
Bsa29I ATCGAT 2 cut(s) 1056, 2550
BsaAI YACGTR 1 cut(s) 1651
BsaHI GRCGYC 1 cut(s) 445
BsaJI CCNNGG 7 cut(s) 243, 295, 716, 768, 1401, 2074, 2895
BsaWI WCCGGW 2 cut(s) 1585, 2426
Bsc4I CCNNNNNNNGG 8 cut(s) 301, 573, 631, 755, 1752, 1804, 2134, 2258
Bse1I ACTGG 3 cut(s) 760, 983, 2263
Bse21I CCTNAGG 1 cut(s) 402
Bse3DI GCAATG 2 cut(s) 636, 2139
BseBI CCWGG 2 cut(s) 750, 2253
BseCI ATCGAT 2 cut(s) 1056, 2550
BseDI CCNNGG 7 cut(s) 243, 295, 716, 768, 1401, 2074, 2895
BseLI CCNNNNNNNGG 8 cut(s) 301, 573, 631, 755, 1752, 1804, 2134, 2258
BseMI GCAATG 2 cut(s) 636, 2139
BseMII CTCAG 9 cut(s) 238, 416, 834, 839, 1290, 1741, 2337, 2342, 2820
BseNI ACTGG 3 cut(s) 760, 983, 2263
BseRI GAGGAG 5 cut(s) 971, 1397, 2228, 2468, 2891
BseXI GCAGC 9 cut(s) 46, 763, 1029, 1169, 1360, 2266, 2523, 2663, 2854
BsgI GTGCAG 1 cut(s) 2610
Bsh1236I CGCG 1 cut(s) 230
BshFI GGCC 4 cut(s) 745, 1400, 2248, 2894
BshNI GGYRCC 1 cut(s) 96
BshVI ATCGAT 2 cut(s) 1056, 2550
BsiHKAI GWGCWC 3 cut(s) 606, 1674, 2109
BsiHKCI CYCGRG 3 cut(s) 243, 1746, 2861
BsiSI CCGG 5 cut(s) 244, 608, 1586, 2111, 2427
BslFI GGGAC 2 cut(s) 589, 2092
BslI CCNNNNNNNGG 8 cut(s) 301, 573, 631, 755, 1752, 1804, 2134, 2258
BsmAI GTCTC 5 cut(s) 1081, 1569, 1794, 2575, 2717
BsmBI CGTCTC 1 cut(s) 2717
BsmFI GGGAC 2 cut(s) 589, 2092
BsmI GAATGC 3 cut(s) 1193, 2687, 2944
BsnI GGCC 4 cut(s) 745, 1400, 2248, 2894
BsoBI CYCGRG 3 cut(s) 243, 1746, 2861
Bsp119I TTCGAA 3 cut(s) 988, 1188, 1597
Bsp1286I GDGCHC 5 cut(s) 22, 32, 606, 1674, 2109
Bsp1720I GCTNAGC 1 cut(s) 249
Bsp19I CCATGG 1 cut(s) 768
BspACI CCGC 8 cut(s) 207, 253, 724, 1077, 1457, 1710, 1756, 2227
BspANI GGCC 4 cut(s) 745, 1400, 2248, 2894
BspCNI CTCAG 9 cut(s) 237, 415, 835, 840, 1291, 1740, 2338, 2343, 2821
BspDI ATCGAT 2 cut(s) 1056, 2550
BspFNI CGCG 1 cut(s) 230
BspHI TCATGA 5 cut(s) 265, 1557, 1768, 1962, 2295
BspLI GGNNCC 6 cut(s) 98, 587, 636, 981, 2090, 2139
BspMAI CTGCAG 1 cut(s) 2056
BspMI ACCTGC 2 cut(s) 2320, 2788
BspPI GGATC 5 cut(s) 974, 987, 1121, 1468, 2615
BspT104I TTCGAA 3 cut(s) 988, 1188, 1597
BspT107I GGYRCC 1 cut(s) 96
BsrDI GCAATG 2 cut(s) 636, 2139
BsrI ACTGG 3 cut(s) 760, 983, 2263
BssECI CCNNGG 7 cut(s) 243, 295, 716, 768, 1401, 2074, 2895
BssNAI GTATAC 1 cut(s) 1487
BssNI GRCGYC 1 cut(s) 445
BssT1I CCWWGG 3 cut(s) 295, 716, 768
Bst1107I GTATAC 1 cut(s) 1487
Bst2UI CCWGG 2 cut(s) 750, 2253
Bst4CI ACNGT 2 cut(s) 311, 829
Bst6I CTCTTC 3 cut(s) 1138, 1425, 2632
BstACI GRCGYC 1 cut(s) 445
BstBAI YACGTR 1 cut(s) 1651
BstBI TTCGAA 3 cut(s) 988, 1188, 1597
BstC8I GCNNGC 7 cut(s) 43, 188, 911, 1075, 1206, 2017, 2414
BstDSI CCRYGG 1 cut(s) 768
BstFNI CGCG 1 cut(s) 230
BstHHI GCGC 2 cut(s) 230, 1135
BstMAI GTCTC 5 cut(s) 1081, 1569, 1794, 2575, 2717
BstNI CCWGG 2 cut(s) 750, 2253
BstSCI CCNGG 6 cut(s) 242, 243, 606, 748, 2109, 2251
BstSFI CTRYAG 3 cut(s) 288, 568, 2052
BstUI CGCG 1 cut(s) 230
BstV1I GCAGC 9 cut(s) 46, 763, 1029, 1169, 1360, 2266, 2523, 2663, 2854
BstV2I GAAGAC 1 cut(s) 228
BstX2I RGATCY 2 cut(s) 979, 1473
BstYI RGATCY 2 cut(s) 979, 1473
BstZ17I GTATAC 1 cut(s) 1487
Bsu15I ATCGAT 2 cut(s) 1056, 2550
Bsu36I CCTNAGG 1 cut(s) 402
BsuRI GGCC 4 cut(s) 745, 1400, 2248, 2894
BsuTUI ATCGAT 2 cut(s) 1056, 2550
BtgI CCRYGG 1 cut(s) 768
BtsI GCAGTG 1 cut(s) 1455
BtsIMutI CAGTG 5 cut(s) 316, 753, 840, 1455, 2343
BveI ACCTGC 2 cut(s) 2320, 2788
Cac8I GCNNGC 7 cut(s) 43, 188, 911, 1075, 1206, 2017, 2414
CaiI CAGNNNCTG 2 cut(s) 755, 2258
CciI TCATGA 5 cut(s) 265, 1557, 1768, 1962, 2295
CfoI GCGC 2 cut(s) 230, 1135
Cfr13I GGNCC 4 cut(s) 634, 743, 2137, 2246
Cfr9I CCCGGG 1 cut(s) 243
ClaI ATCGAT 2 cut(s) 1056, 2550
CseI GACGC 3 cut(s) 453, 1956, 2734
Csp6I GTAC 4 cut(s) 97, 562, 694, 2197
CviQI GTAC 4 cut(s) 97, 562, 694, 2197
DriI GACNNNNNGTC 1 cut(s) 283
Eam1104I CTCTTC 3 cut(s) 1138, 1425, 2632
Eam1105I GACNNNNNGTC 1 cut(s) 283
EarI CTCTTC 3 cut(s) 1138, 1425, 2632
EciI GGCGGA 2 cut(s) 196, 1699
Eco130I CCWWGG 3 cut(s) 295, 716, 768
Eco24I GRGCYC 2 cut(s) 22, 32
Eco32I GATATC 4 cut(s) 457, 507, 1960, 2010
Eco47I GGWCC 2 cut(s) 634, 2137
Eco57I CTGAAG 2 cut(s) 1463, 1487
Eco81I CCTNAGG 1 cut(s) 402
Eco88I CYCGRG 3 cut(s) 243, 1746, 2861
EcoRI GAATTC 2 cut(s) 167, 2065
EcoRII CCWGG 2 cut(s) 748, 2251
EcoRV GATATC 4 cut(s) 457, 507, 1960, 2010
EcoT14I CCWWGG 3 cut(s) 295, 716, 768
EcoT38I GRGCYC 2 cut(s) 22, 32
ErhI CCWWGG 3 cut(s) 295, 716, 768
Esp3I CGTCTC 1 cut(s) 2717
FaqI GGGAC 2 cut(s) 589, 2092
FauI CCCGC 2 cut(s) 717, 2220
FbaI TGATCA 1 cut(s) 1981
FblI GTMKAC 4 cut(s) 138, 764, 1486, 2267
FriOI GRGCYC 2 cut(s) 22, 32
FspBI CTAG 6 cut(s) 626, 1209, 1628, 1793, 2129, 2703
GlaI GCGC 2 cut(s) 229, 1134
HaeIII GGCC 4 cut(s) 745, 1400, 2248, 2894
HapII CCGG 5 cut(s) 244, 608, 1586, 2111, 2427
HgaI GACGC 3 cut(s) 453, 1956, 2734
HhaI GCGC 2 cut(s) 230, 1135
Hin1I GRCGYC 1 cut(s) 445
Hin6I GCGC 2 cut(s) 228, 1133
HinP1I GCGC 2 cut(s) 228, 1133
HindIII AAGCTT 2 cut(s) 2033, 2357
HinfI GANTC 9 cut(s) 376, 418, 1229, 1282, 1331, 1337, 2474, 2524, 2831
HpaII CCGG 5 cut(s) 244, 608, 1586, 2111, 2427
HphI GGTGA 7 cut(s) 46, 163, 523, 841, 1816, 1876, 2533
Hpy166II GTNNAC 7 cut(s) 139, 765, 1101, 1487, 2268, 2595, 2611
Hpy188I TCNGA 8 cut(s) 381, 680, 1336, 1478, 2364, 2484, 2636, 2815
Hpy8I GTNNAC 7 cut(s) 139, 765, 1101, 1487, 2268, 2595, 2611
Hpy99I CGWCG 1 cut(s) 829
HpyCH4III ACNGT 2 cut(s) 311, 829
HpyCH4IV ACGT 5 cut(s) 434, 1650, 1847, 1937, 2544
HpyCH4V TGCA 8 cut(s) 701, 1591, 1654, 2054, 2204, 2627, 2792, 2942
HpySE526I ACGT 5 cut(s) 434, 1650, 1847, 1937, 2544
Hsp92I GRCGYC 1 cut(s) 445
HspAI GCGC 2 cut(s) 228, 1133
KpnI GGTACC 1 cut(s) 100
Ksp22I TGATCA 1 cut(s) 1981
LmnI GCTCC 2 cut(s) 585, 2088
Lsp1109I GCAGC 9 cut(s) 46, 763, 1029, 1169, 1360, 2266, 2523, 2663, 2854
LweI GCATC 4 cut(s) 620, 1299, 2793, 2815
MaeI CTAG 6 cut(s) 626, 1209, 1628, 1793, 2129, 2703
MaeII ACGT 5 cut(s) 434, 1650, 1847, 1937, 2544
MaeIII GTNAC 3 cut(s) 319, 1027, 2521
MflI RGATCY 2 cut(s) 979, 1473
MhlI GDGCHC 5 cut(s) 22, 32, 606, 1674, 2109
MlyI GAGTC 3 cut(s) 1276, 2518, 2840
MmeI TCCRAC 1 cut(s) 94
MroXI GAANNNNTTC 1 cut(s) 1637
MseI TTAA 4 cut(s) 257, 342, 821, 2334
MspI CCGG 5 cut(s) 244, 608, 1586, 2111, 2427
MspR9I CCNGG 6 cut(s) 244, 245, 608, 750, 2111, 2253
Mva1269I GAATGC 3 cut(s) 1193, 2687, 2944
MvaI CCWGG 2 cut(s) 750, 2253
MvnI CGCG 1 cut(s) 230
NciI CCSGG 4 cut(s) 244, 245, 608, 2111
NcoI CCATGG 1 cut(s) 768
NlaIV GGNNCC 6 cut(s) 98, 587, 636, 981, 2090, 2139
NmeAIII GCCGAG 3 cut(s) 1426, 2400, 2920
NmuCI GTSAC 3 cut(s) 319, 1027, 2521
NspV TTCGAA 3 cut(s) 988, 1188, 1597
PaeR7I CTCGAG 1 cut(s) 2861
PagI TCATGA 5 cut(s) 265, 1557, 1768, 1962, 2295
PaqCI CACCTGC 1 cut(s) 2788
PcsI WCGNNNNNNNCGW 4 cut(s) 38, 1541, 1647, 2859
PctI GAATGC 3 cut(s) 1193, 2687, 2944
PdmI GAANNNNTTC 1 cut(s) 1637
PfeI GAWTC 6 cut(s) 376, 418, 1229, 1331, 1337, 2474
PflFI GACNNNGTC 1 cut(s) 1969
PflMI CCANNNNNTGG 2 cut(s) 755, 2258
PleI GAGTC 3 cut(s) 1276, 2518, 2839
PpsI GAGTC 3 cut(s) 1276, 2518, 2839
Ppu21I YACGTR 1 cut(s) 1651
PshBI ATTAAT 1 cut(s) 342
PsiI TTATAA 1 cut(s) 1929
Psp1406I AACGTT 1 cut(s) 2544
Psp6I CCWGG 2 cut(s) 748, 2251
PspGI CCWGG 2 cut(s) 748, 2251
PspN4I GGNNCC 6 cut(s) 98, 587, 636, 981, 2090, 2139
PspPI GGNCC 4 cut(s) 634, 743, 2137, 2246
PstI CTGCAG 1 cut(s) 2056
PstNI CAGNNNCTG 2 cut(s) 755, 2258
PsuI RGATCY 2 cut(s) 979, 1473
PsyI GACNNNGTC 1 cut(s) 1969
RsaI GTAC 4 cut(s) 98, 563, 695, 2198
RsaNI GTAC 4 cut(s) 97, 562, 694, 2197
SaqAI TTAA 4 cut(s) 257, 342, 821, 2334
Sau96I GGNCC 4 cut(s) 634, 743, 2137, 2246
ScaI AGTACT 2 cut(s) 695, 2198
SchI GAGTC 3 cut(s) 1276, 2518, 2840
ScrFI CCNGG 6 cut(s) 244, 245, 608, 750, 2111, 2253
SduI GDGCHC 5 cut(s) 22, 32, 606, 1674, 2109
SfaNI GCATC 4 cut(s) 620, 1299, 2793, 2815
SfcI CTRYAG 3 cut(s) 288, 568, 2052
Sfr274I CTCGAG 1 cut(s) 2861
SfuI TTCGAA 3 cut(s) 988, 1188, 1597
SinI GGWCC 2 cut(s) 634, 2137
SlaI CTCGAG 1 cut(s) 2861
SmaI CCCGGG 1 cut(s) 245
SmlI CTYRAG 3 cut(s) 1367, 1760, 2861
SmoI CTYRAG 3 cut(s) 1367, 1760, 2861
SsiI CCGC 8 cut(s) 207, 253, 724, 1077, 1457, 1710, 1756, 2227
SspMI CTAG 6 cut(s) 626, 1209, 1628, 1793, 2129, 2703
StyD4I CCNGG 6 cut(s) 242, 243, 606, 748, 2109, 2251
StyI CCWWGG 3 cut(s) 295, 716, 768
TaaI ACNGT 2 cut(s) 311, 829
TaiI ACGT 5 cut(s) 437, 1653, 1850, 1940, 2547
TatI WGTACW 2 cut(s) 693, 2196
TauI GCSGC 2 cut(s) 1460, 1759
TfiI GAWTC 6 cut(s) 376, 418, 1229, 1331, 1337, 2474
Tru1I TTAA 4 cut(s) 257, 342, 821, 2334
Tru9I TTAA 4 cut(s) 257, 342, 821, 2334
TscAI CASTG 5 cut(s) 316, 760, 847, 1455, 2350
TseFI GTSAC 3 cut(s) 319, 1027, 2521
TseI GCWGC 9 cut(s) 59, 776, 1042, 1182, 1373, 2279, 2536, 2676, 2867
Tsp45I GTSAC 3 cut(s) 319, 1027, 2521
TspDTI ATGAA 9 cut(s) 180, 339, 342, 375, 1546, 1845, 1878, 2058, 2312
TspGWI ACGGA 1 cut(s) 2783
TspMI CCCGGG 1 cut(s) 243
TspRI CASTG 5 cut(s) 316, 760, 847, 1455, 2350
Tth111I GACNNNGTC 1 cut(s) 1969
Van91I CCANNNNNTGG 2 cut(s) 755, 2258
VpaK11BI GGWCC 2 cut(s) 634, 2137
VspI ATTAAT 1 cut(s) 342
XapI RAATTY 6 cut(s) 167, 329, 1832, 2065, 2797, 2932
XhoI CTCGAG 1 cut(s) 2861
XmaI CCCGGG 1 cut(s) 243
XmiI GTMKAC 4 cut(s) 138, 764, 1486, 2267
XmnI GAANNNNTTC 1 cut(s) 1637
XspI CTAG 6 cut(s) 626, 1209, 1628, 1793, 2129, 2703
ZrmI AGTACT 2 cut(s) 695, 2198
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.