Belongs to the AAA ATPase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 449 |
| AclWI | GGATC | 2 cut(s) | 665, 678 |
| AcsI | RAATTY | 2 cut(s) | 29, 838 |
| AcvI | CACGTG | 1 cut(s) | 168 |
| AfaI | GTAC | 1 cut(s) | 389 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 449, 881 |
| AgsI | TTSAA | 8 cut(s) | 739, 814, 838, 872, 964, 977, 1084, 1175 |
| AjnI | CCWGG | 3 cut(s) | 59, 442, 714 |
| AluBI | AGCT | 8 cut(s) | 46, 133, 235, 282, 514, 905, 980, 1142 |
| AluI | AGCT | 8 cut(s) | 46, 133, 235, 282, 514, 905, 980, 1142 |
| Alw21I | GWGCWC | 1 cut(s) | 284 |
| Alw26I | GTCTC | 1 cut(s) | 881 |
| AlwI | GGATC | 2 cut(s) | 665, 678 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 449 |
| AoxI | GGCC | 1 cut(s) | 437 |
| ApeKI | GCWGC | 1 cut(s) | 470 |
| ApoI | RAATTY | 2 cut(s) | 29, 838 |
| AspA2I | CCTAGG | 1 cut(s) | 74 |
| AspLEI | GCGC | 1 cut(s) | 71 |
| AspS9I | GGNCC | 1 cut(s) | 437 |
| AsuHPI | GGTGA | 2 cut(s) | 31, 290 |
| AvrII | CCTAGG | 1 cut(s) | 74 |
| BamHI | GGATCC | 1 cut(s) | 670 |
| BanII | GRGCYC | 1 cut(s) | 284 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 374, 406 |
| BbrPI | CACGTG | 1 cut(s) | 168 |
| Bbv12I | GWGCWC | 1 cut(s) | 284 |
| BbvCI | CCTCAGC | 1 cut(s) | 1017 |
| BbvI | GCAGC | 1 cut(s) | 457 |
| BccI | CCATC | 3 cut(s) | 743, 763, 959 |
| BciT130I | CCWGG | 3 cut(s) | 61, 444, 716 |
| BclI | TGATCA | 1 cut(s) | 295 |
| BcoDI | GTCTC | 1 cut(s) | 881 |
| BfaI | CTAG | 5 cut(s) | 75, 258, 729, 902, 983 |
| BfmI | CTRYAG | 1 cut(s) | 285 |
| BisI | GCNGC | 1 cut(s) | 471 |
| BlnI | CCTAGG | 1 cut(s) | 74 |
| BlsI | GCNGC | 1 cut(s) | 472 |
| BmcAI | AGTACT | 1 cut(s) | 389 |
| Bme1390I | CCNGG | 3 cut(s) | 61, 444, 716 |
| BmgT120I | GGNCC | 1 cut(s) | 437 |
| BmiI | GGNNCC | 2 cut(s) | 330, 672 |
| BmrFI | CCNGG | 3 cut(s) | 61, 444, 716 |
| BmsI | GCATC | 6 cut(s) | 113, 137, 915, 937, 1039, 1141 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1017 |
| BpuEI | CTTGAG | 1 cut(s) | 530 |
| BsaAI | YACGTR | 1 cut(s) | 168 |
| BsaI | GGTCTC | 1 cut(s) | 881 |
| BsaJI | CCNNGG | 3 cut(s) | 34, 74, 410 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 449, 881 |
| Bse1I | ACTGG | 1 cut(s) | 454 |
| Bse3DI | GCAATG | 1 cut(s) | 330 |
| BseBI | CCWGG | 3 cut(s) | 61, 444, 716 |
| BseDI | CCNNGG | 3 cut(s) | 34, 74, 410 |
| BseGI | GGATG | 4 cut(s) | 10, 774, 952, 1054 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 449, 881 |
| BseMI | GCAATG | 1 cut(s) | 330 |
| BseMII | CTCAG | 3 cut(s) | 119, 533, 1008 |
| BseNI | ACTGG | 1 cut(s) | 454 |
| BseRI | GAGGAG | 2 cut(s) | 1013, 1115 |
| BseXI | GCAGC | 1 cut(s) | 457 |
| BshFI | GGCC | 1 cut(s) | 439 |
| BsiHKAI | GWGCWC | 1 cut(s) | 284 |
| BslFI | GGGAC | 1 cut(s) | 170 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 449, 881 |
| BsmAI | GTCTC | 1 cut(s) | 881 |
| BsmFI | GGGAC | 1 cut(s) | 170 |
| BsmI | GAATGC | 1 cut(s) | 809 |
| BsnI | GGCC | 1 cut(s) | 439 |
| Bso31I | GGTCTC | 1 cut(s) | 881 |
| Bsp1286I | GDGCHC | 1 cut(s) | 284 |
| Bsp143I | GATC | 6 cut(s) | 295, 505, 538, 585, 610, 670 |
| BspANI | GGCC | 1 cut(s) | 439 |
| BspCNI | CTCAG | 3 cut(s) | 118, 534, 1009 |
| BspHI | TCATGA | 1 cut(s) | 486 |
| BspLI | GGNNCC | 2 cut(s) | 330, 672 |
| BspPI | GGATC | 2 cut(s) | 665, 678 |
| BspQI | GCTCTTC | 1 cut(s) | 521 |
| BspTNI | GGTCTC | 1 cut(s) | 881 |
| BsrDI | GCAATG | 1 cut(s) | 330 |
| BsrI | ACTGG | 1 cut(s) | 454 |
| BssECI | CCNNGG | 3 cut(s) | 34, 74, 410 |
| BssMI | GATC | 6 cut(s) | 295, 505, 538, 585, 610, 670 |
| BssT1I | CCWWGG | 3 cut(s) | 34, 74, 410 |
| Bst2UI | CCWGG | 3 cut(s) | 61, 444, 716 |
| Bst4CI | ACNGT | 1 cut(s) | 523 |
| Bst6I | CTCTTC | 4 cut(s) | 9, 113, 521, 1113 |
| BstBAI | YACGTR | 1 cut(s) | 168 |
| BstC8I | GCNNGC | 1 cut(s) | 822 |
| BstDEI | CTNAG | 3 cut(s) | 105, 542, 1017 |
| BstF5I | GGATG | 4 cut(s) | 10, 774, 952, 1054 |
| BstHHI | GCGC | 1 cut(s) | 71 |
| BstKTI | GATC | 6 cut(s) | 298, 508, 541, 588, 613, 673 |
| BstMAI | GTCTC | 1 cut(s) | 881 |
| BstMBI | GATC | 6 cut(s) | 295, 505, 538, 585, 610, 670 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 232, 711, 911 |
| BstNI | CCWGG | 3 cut(s) | 61, 444, 716 |
| BstSCI | CCNGG | 3 cut(s) | 59, 442, 714 |
| BstSFI | CTRYAG | 1 cut(s) | 285 |
| BstV1I | GCAGC | 1 cut(s) | 457 |
| BstX2I | RGATCY | 1 cut(s) | 670 |
| BstYI | RGATCY | 1 cut(s) | 670 |
| BsuRI | GGCC | 1 cut(s) | 439 |
| BtsCI | GGATG | 4 cut(s) | 10, 774, 952, 1054 |
| BtsI | GCAGTG | 1 cut(s) | 219 |
| BtsIMutI | CAGTG | 4 cut(s) | 219, 447, 528, 534 |
| Cac8I | GCNNGC | 1 cut(s) | 822 |
| CaiI | CAGNNNCTG | 1 cut(s) | 449 |
| CciI | TCATGA | 1 cut(s) | 486 |
| CfoI | GCGC | 1 cut(s) | 71 |
| Cfr13I | GGNCC | 1 cut(s) | 437 |
| Csp6I | GTAC | 1 cut(s) | 388 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 448, 483 |
| CviAII | CATG | 5 cut(s) | 185, 364, 463, 475, 487 |
| CviQI | GTAC | 1 cut(s) | 388 |
| DdeI | CTNAG | 3 cut(s) | 105, 542, 1017 |
| DpnI | GATC | 6 cut(s) | 297, 507, 540, 587, 612, 672 |
| DpnII | GATC | 6 cut(s) | 295, 505, 538, 585, 610, 670 |
| Eam1104I | CTCTTC | 4 cut(s) | 9, 113, 521, 1113 |
| EarI | CTCTTC | 4 cut(s) | 9, 113, 521, 1113 |
| Ecl136II | GAGCTC | 1 cut(s) | 282 |
| Eco130I | CCWWGG | 3 cut(s) | 34, 74, 410 |
| Eco24I | GRGCYC | 1 cut(s) | 284 |
| Eco31I | GGTCTC | 1 cut(s) | 881 |
| Eco53kI | GAGCTC | 1 cut(s) | 282 |
| Eco72I | CACGTG | 1 cut(s) | 168 |
| EcoICRI | GAGCTC | 1 cut(s) | 282 |
| EcoRI | GAATTC | 1 cut(s) | 29 |
| EcoRII | CCWGG | 3 cut(s) | 59, 442, 714 |
| EcoT14I | CCWWGG | 3 cut(s) | 34, 74, 410 |
| EcoT38I | GRGCYC | 1 cut(s) | 284 |
| ErhI | CCWWGG | 3 cut(s) | 34, 74, 410 |
| FaeI | CATG | 5 cut(s) | 188, 367, 466, 478, 490 |
| FaqI | GGGAC | 1 cut(s) | 170 |
| FatI | CATG | 5 cut(s) | 184, 363, 462, 474, 486 |
| FbaI | TGATCA | 1 cut(s) | 295 |
| Fnu4HI | GCNGC | 1 cut(s) | 471 |
| FokI | GGATG | 4 cut(s) | 17, 781, 959, 1061 |
| FriOI | GRGCYC | 1 cut(s) | 284 |
| Fsp4HI | GCNGC | 1 cut(s) | 471 |
| FspBI | CTAG | 5 cut(s) | 75, 258, 729, 902, 983 |
| GlaI | GCGC | 1 cut(s) | 70 |
| GluI | GCNGC | 1 cut(s) | 471 |
| HaeIII | GGCC | 1 cut(s) | 439 |
| HhaI | GCGC | 1 cut(s) | 71 |
| Hin1II | CATG | 5 cut(s) | 188, 367, 466, 478, 490 |
| Hin6I | GCGC | 1 cut(s) | 69 |
| HinP1I | GCGC | 1 cut(s) | 69 |
| HindIII | AAGCTT | 1 cut(s) | 233 |
| HinfI | GANTC | 4 cut(s) | 217, 845, 898, 1103 |
| HphI | GGTGA | 2 cut(s) | 31, 290 |
| Hpy166II | GTNNAC | 2 cut(s) | 459, 1060 |
| Hpy188I | TCNGA | 4 cut(s) | 112, 543, 863, 1133 |
| Hpy188III | TCNNGA | 6 cut(s) | 179, 258, 403, 487, 509, 983 |
| Hpy8I | GTNNAC | 2 cut(s) | 459, 1060 |
| HpyAV | CCTTC | 8 cut(s) | 191, 376, 631, 686, 759, 1006, 1030, 1078 |
| HpyCH4III | ACNGT | 1 cut(s) | 523 |
| HpyCH4IV | ACGT | 1 cut(s) | 167 |
| HpyCH4V | TGCA | 4 cut(s) | 305, 824, 992, 1052 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 232, 711, 911 |
| HpyF3I | CTNAG | 3 cut(s) | 105, 542, 1017 |
| HpySE526I | ACGT | 1 cut(s) | 167 |
| Hsp92II | CATG | 5 cut(s) | 188, 367, 466, 478, 490 |
| HspAI | GCGC | 1 cut(s) | 69 |
| Ksp22I | TGATCA | 1 cut(s) | 295 |
| Kzo9I | GATC | 6 cut(s) | 295, 505, 538, 585, 610, 670 |
| LguI | GCTCTTC | 1 cut(s) | 521 |
| LmnI | GCTCC | 2 cut(s) | 279, 328 |
| Lsp1109I | GCAGC | 1 cut(s) | 457 |
| LweI | GCATC | 6 cut(s) | 113, 137, 915, 937, 1039, 1141 |
| MaeI | CTAG | 5 cut(s) | 75, 258, 729, 902, 983 |
| MaeII | ACGT | 1 cut(s) | 167 |
| MaeIII | GTNAC | 2 cut(s) | 19, 718 |
| MalI | GATC | 6 cut(s) | 297, 507, 540, 587, 612, 672 |
| MboI | GATC | 6 cut(s) | 295, 505, 538, 585, 610, 670 |
| MflI | RGATCY | 1 cut(s) | 670 |
| MhlI | GDGCHC | 1 cut(s) | 284 |
| MluCI | AATT | 6 cut(s) | 29, 785, 838, 854, 968, 1079 |
| MlyI | GAGTC | 3 cut(s) | 226, 892, 1112 |
| MmeI | TCCRAC | 1 cut(s) | 698 |
| MseI | TTAA | 3 cut(s) | 42, 243, 1044 |
| MslI | CAYNNNNRTG | 1 cut(s) | 323 |
| MspR9I | CCNGG | 3 cut(s) | 61, 444, 716 |
| Mva1269I | GAATGC | 1 cut(s) | 809 |
| MvaI | CCWGG | 3 cut(s) | 61, 444, 716 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 232, 711, 911 |
| NdeII | GATC | 6 cut(s) | 295, 505, 538, 585, 610, 670 |
| NlaIII | CATG | 5 cut(s) | 188, 367, 466, 478, 490 |
| NlaIV | GGNNCC | 2 cut(s) | 330, 672 |
| NmuCI | GTSAC | 1 cut(s) | 19 |
| PagI | TCATGA | 1 cut(s) | 486 |
| PciSI | GCTCTTC | 1 cut(s) | 521 |
| PctI | GAATGC | 1 cut(s) | 809 |
| PfeI | GAWTC | 1 cut(s) | 845 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 449 |
| PfoI | TCCNGGA | 1 cut(s) | 59 |
| PkrI | GCNGC | 1 cut(s) | 472 |
| PleI | GAGTC | 3 cut(s) | 225, 892, 1111 |
| PmaCI | CACGTG | 1 cut(s) | 168 |
| PmlI | CACGTG | 1 cut(s) | 168 |
| PpsI | GAGTC | 3 cut(s) | 225, 892, 1111 |
| Ppu21I | YACGTR | 1 cut(s) | 168 |
| Psp124BI | GAGCTC | 1 cut(s) | 284 |
| Psp6I | CCWGG | 3 cut(s) | 59, 442, 714 |
| PspCI | CACGTG | 1 cut(s) | 168 |
| PspGI | CCWGG | 3 cut(s) | 59, 442, 714 |
| PspN4I | GGNNCC | 2 cut(s) | 330, 672 |
| PspPI | GGNCC | 1 cut(s) | 437 |
| PstNI | CAGNNNCTG | 1 cut(s) | 449 |
| PsuI | RGATCY | 1 cut(s) | 670 |
| RsaI | GTAC | 1 cut(s) | 389 |
| RsaNI | GTAC | 1 cut(s) | 388 |
| RseI | CAYNNNNRTG | 1 cut(s) | 323 |
| SacI | GAGCTC | 1 cut(s) | 284 |
| SapI | GCTCTTC | 1 cut(s) | 521 |
| SaqAI | TTAA | 3 cut(s) | 42, 243, 1044 |
| SatI | GCNGC | 1 cut(s) | 471 |
| Sau3AI | GATC | 6 cut(s) | 295, 505, 538, 585, 610, 670 |
| Sau96I | GGNCC | 1 cut(s) | 437 |
| ScaI | AGTACT | 1 cut(s) | 389 |
| SchI | GAGTC | 3 cut(s) | 226, 892, 1112 |
| ScrFI | CCNGG | 3 cut(s) | 61, 444, 716 |
| SduI | GDGCHC | 1 cut(s) | 284 |
| SfaNI | GCATC | 6 cut(s) | 113, 137, 915, 937, 1039, 1141 |
| SfcI | CTRYAG | 1 cut(s) | 285 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 323 |
| SmlI | CTYRAG | 1 cut(s) | 509 |
| SmoI | CTYRAG | 1 cut(s) | 509 |
| Sse9I | AATT | 6 cut(s) | 29, 785, 838, 854, 968, 1079 |
| SspMI | CTAG | 5 cut(s) | 75, 258, 729, 902, 983 |
| SstI | GAGCTC | 1 cut(s) | 284 |
| StyD4I | CCNGG | 3 cut(s) | 59, 442, 714 |
| StyI | CCWWGG | 3 cut(s) | 34, 74, 410 |
| TaaI | ACNGT | 1 cut(s) | 523 |
| TaiI | ACGT | 1 cut(s) | 170 |
| TaqI | TCGA | 1 cut(s) | 588 |
| TasI | AATT | 6 cut(s) | 29, 785, 838, 854, 968, 1079 |
| TatI | WGTACW | 1 cut(s) | 387 |
| TfiI | GAWTC | 1 cut(s) | 845 |
| Tru1I | TTAA | 3 cut(s) | 42, 243, 1044 |
| Tru9I | TTAA | 3 cut(s) | 42, 243, 1044 |
| TscAI | CASTG | 4 cut(s) | 219, 454, 528, 541 |
| TseFI | GTSAC | 1 cut(s) | 19 |
| TseI | GCWGC | 1 cut(s) | 470 |
| Tsp45I | GTSAC | 1 cut(s) | 19 |
| TspDTI | ATGAA | 8 cut(s) | 27, 42, 75, 503, 663, 733, 819, 1131 |
| TspRI | CASTG | 4 cut(s) | 219, 454, 528, 541 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 449 |
| XapI | RAATTY | 2 cut(s) | 29, 838 |
| XbaI | TCTAGA | 2 cut(s) | 257, 982 |
| XmaJI | CCTAGG | 1 cut(s) | 74 |
| XspI | CTAG | 5 cut(s) | 75, 258, 729, 902, 983 |
| ZrmI | AGTACT | 1 cut(s) | 389 |