Belongs to the AAA ATPase family
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 896 |
| AarI | CACCTGC | 1 cut(s) | 320 |
| Acc36I | ACCTGC | 3 cut(s) | 190, 320, 1293 |
| AccB1I | GGYRCC | 1 cut(s) | 543 |
| AccBSI | CCGCTC | 1 cut(s) | 751 |
| AccI | GTMKAC | 2 cut(s) | 797, 1012 |
| AccIII | TCCGGA | 2 cut(s) | 226, 1426 |
| AciI | CCGC | 2 cut(s) | 392, 751 |
| AclWI | GGATC | 3 cut(s) | 581, 594, 918 |
| AcoI | YGGCCR | 4 cut(s) | 3, 631, 1054, 1270 |
| AcsI | RAATTY | 4 cut(s) | 246, 287, 853, 1154 |
| AcuI | CTGAAG | 2 cut(s) | 688, 744 |
| AfaI | GTAC | 5 cut(s) | 60, 176, 411, 820, 1190 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 18, 575, 1063, 1273 |
| AgsI | TTSAA | 6 cut(s) | 34, 43, 65, 305, 1382, 1420 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 177 |
| AjnI | CCWGG | 3 cut(s) | 466, 1211, 1272 |
| Alw21I | GWGCWC | 2 cut(s) | 927, 1342 |
| Alw26I | GTCTC | 1 cut(s) | 643 |
| AlwI | GGATC | 3 cut(s) | 581, 594, 918 |
| Ama87I | CYCGRG | 1 cut(s) | 104 |
| Aor13HI | TCCGGA | 2 cut(s) | 226, 1426 |
| AoxI | GGCC | 5 cut(s) | 3, 631, 1054, 1140, 1270 |
| ApeKI | GCWGC | 4 cut(s) | 238, 326, 1299, 1406 |
| ApoI | RAATTY | 4 cut(s) | 246, 287, 853, 1154 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 38, 701 |
| AspLEI | GCGC | 2 cut(s) | 276, 1111 |
| AspS9I | GGNCC | 2 cut(s) | 578, 1141 |
| AsuC2I | CCSGG | 1 cut(s) | 862 |
| AsuHPI | GGTGA | 4 cut(s) | 347, 898, 1024, 1256 |
| AvaI | CYCGRG | 1 cut(s) | 104 |
| AvaII | GGWCC | 1 cut(s) | 578 |
| AxyI | CCTNAGG | 1 cut(s) | 415 |
| BalI | TGGCCA | 3 cut(s) | 5, 1056, 1272 |
| BamHI | GGATCC | 1 cut(s) | 586 |
| BanI | GGYRCC | 1 cut(s) | 543 |
| BanII | GRGCYC | 3 cut(s) | 105, 927, 1342 |
| Bbv12I | GWGCWC | 2 cut(s) | 927, 1342 |
| BbvI | GCAGC | 4 cut(s) | 225, 313, 1286, 1418 |
| BccI | CCATC | 6 cut(s) | 77, 332, 1046, 1196, 1237, 1243 |
| BceAI | ACGGC | 1 cut(s) | 618 |
| BciT130I | CCWGG | 3 cut(s) | 468, 1213, 1274 |
| BclI | TGATCA | 1 cut(s) | 1180 |
| BcnI | CCSGG | 1 cut(s) | 862 |
| BcoDI | GTCTC | 1 cut(s) | 643 |
| BfaI | CTAG | 3 cut(s) | 260, 972, 1335 |
| BfmI | CTRYAG | 2 cut(s) | 327, 1300 |
| BfuAI | ACCTGC | 3 cut(s) | 190, 320, 1293 |
| BisI | GCNGC | 4 cut(s) | 239, 327, 1300, 1407 |
| BlsI | GCNGC | 4 cut(s) | 240, 328, 1301, 1408 |
| Bme1390I | CCNGG | 4 cut(s) | 468, 862, 1213, 1274 |
| Bme18I | GGWCC | 1 cut(s) | 578 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 177 |
| BmeT110I | CYCGRG | 1 cut(s) | 104 |
| BmgT120I | GGNCC | 2 cut(s) | 578, 1141 |
| BmiI | GGNNCC | 5 cut(s) | 225, 517, 545, 588, 1209 |
| BmrFI | CCNGG | 4 cut(s) | 468, 862, 1213, 1274 |
| BmsI | GCATC | 3 cut(s) | 709, 769, 951 |
| Bpu10I | CCTNAGC | 2 cut(s) | 1137, 1341 |
| BpuEI | CTTGAG | 2 cut(s) | 254, 623 |
| BpuMI | CCSGG | 1 cut(s) | 862 |
| BsaAI | YACGTR | 2 cut(s) | 1010, 1188 |
| BsaJI | CCNNGG | 6 cut(s) | 519, 882, 904, 995, 1211, 1267 |
| BsaWI | WCCGGW | 2 cut(s) | 226, 1426 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 18, 575, 1063, 1273 |
| Bse1I | ACTGG | 3 cut(s) | 113, 200, 427 |
| Bse21I | CCTNAGG | 1 cut(s) | 415 |
| Bse3DI | GCAATG | 2 cut(s) | 283, 591 |
| BseAI | TCCGGA | 2 cut(s) | 226, 1426 |
| BseBI | CCWGG | 3 cut(s) | 468, 1213, 1274 |
| BseDI | CCNNGG | 6 cut(s) | 519, 882, 904, 995, 1211, 1267 |
| BseGI | GGATG | 4 cut(s) | 388, 564, 724, 760 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 18, 575, 1063, 1273 |
| BseMI | GCAATG | 2 cut(s) | 283, 591 |
| BseMII | CTCAG | 5 cut(s) | 305, 863, 1151, 1355, 1377 |
| BseNI | ACTGG | 3 cut(s) | 113, 200, 427 |
| BseRI | GAGGAG | 2 cut(s) | 176, 917 |
| BseXI | GCAGC | 4 cut(s) | 225, 313, 1286, 1418 |
| BseYI | CCCAGC | 1 cut(s) | 450 |
| BsgI | GTGCAG | 2 cut(s) | 224, 1425 |
| BshFI | GGCC | 5 cut(s) | 5, 633, 1056, 1142, 1272 |
| BshNI | GGYRCC | 1 cut(s) | 543 |
| BsiHKAI | GWGCWC | 2 cut(s) | 927, 1342 |
| BsiHKCI | CYCGRG | 1 cut(s) | 104 |
| BsiSI | CCGG | 3 cut(s) | 227, 862, 1427 |
| BslFI | GGGAC | 1 cut(s) | 1138 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 18, 575, 1063, 1273 |
| BsmAI | GTCTC | 1 cut(s) | 643 |
| BsmFI | GGGAC | 1 cut(s) | 1138 |
| BsmI | GAATGC | 1 cut(s) | 1028 |
| BsnI | GGCC | 5 cut(s) | 5, 633, 1056, 1142, 1272 |
| BsoBI | CYCGRG | 1 cut(s) | 104 |
| Bsp1286I | GDGCHC | 3 cut(s) | 105, 927, 1342 |
| Bsp13I | TCCGGA | 2 cut(s) | 226, 1426 |
| Bsp1407I | TGTACA | 1 cut(s) | 818 |
| Bsp143I | GATC | 5 cut(s) | 373, 475, 586, 910, 1180 |
| Bsp19I | CCATGG | 1 cut(s) | 904 |
| BspACI | CCGC | 2 cut(s) | 392, 751 |
| BspANI | GGCC | 5 cut(s) | 5, 633, 1056, 1142, 1272 |
| BspCNI | CTCAG | 5 cut(s) | 306, 862, 1150, 1354, 1376 |
| BspEI | TCCGGA | 2 cut(s) | 226, 1426 |
| BspHI | TCATGA | 1 cut(s) | 552 |
| BspLI | GGNNCC | 5 cut(s) | 225, 517, 545, 588, 1209 |
| BspMAI | CTGCAG | 2 cut(s) | 331, 1304 |
| BspMI | ACCTGC | 3 cut(s) | 190, 320, 1293 |
| BspPI | GGATC | 3 cut(s) | 581, 594, 918 |
| BspQI | GCTCTTC | 1 cut(s) | 915 |
| BspT107I | GGYRCC | 1 cut(s) | 543 |
| BsrBI | CCGCTC | 1 cut(s) | 751 |
| BsrDI | GCAATG | 2 cut(s) | 283, 591 |
| BsrGI | TGTACA | 1 cut(s) | 818 |
| BsrI | ACTGG | 3 cut(s) | 113, 200, 427 |
| BssECI | CCNNGG | 6 cut(s) | 519, 882, 904, 995, 1211, 1267 |
| BssMI | GATC | 5 cut(s) | 373, 475, 586, 910, 1180 |
| BssT1I | CCWWGG | 5 cut(s) | 519, 882, 904, 995, 1267 |
| Bst2UI | CCWGG | 3 cut(s) | 468, 1213, 1274 |
| Bst4CI | ACNGT | 3 cut(s) | 179, 409, 1246 |
| Bst6I | CTCTTC | 4 cut(s) | 29, 213, 915, 1341 |
| BstAUI | TGTACA | 1 cut(s) | 818 |
| BstBAI | YACGTR | 2 cut(s) | 1010, 1188 |
| BstC8I | GCNNGC | 5 cut(s) | 349, 353, 542, 624, 1107 |
| BstDEI | CTNAG | 7 cut(s) | 314, 415, 849, 1137, 1341, 1363, 1451 |
| BstDSI | CCRYGG | 1 cut(s) | 904 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1271 |
| BstF5I | GGATG | 4 cut(s) | 388, 564, 724, 760 |
| BstHHI | GCGC | 2 cut(s) | 276, 1111 |
| BstKTI | GATC | 5 cut(s) | 376, 478, 589, 913, 1183 |
| BstMAI | GTCTC | 1 cut(s) | 643 |
| BstMBI | GATC | 5 cut(s) | 373, 475, 586, 910, 1180 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 757, 878 |
| BstNI | CCWGG | 3 cut(s) | 468, 1213, 1274 |
| BstNSI | RCATGY | 2 cut(s) | 485, 626 |
| BstSCI | CCNGG | 4 cut(s) | 466, 860, 1211, 1272 |
| BstSFI | CTRYAG | 2 cut(s) | 327, 1300 |
| BstSNI | TACGTA | 1 cut(s) | 1188 |
| BstV1I | GCAGC | 4 cut(s) | 225, 313, 1286, 1418 |
| BstX2I | RGATCY | 1 cut(s) | 586 |
| BstYI | RGATCY | 1 cut(s) | 586 |
| Bsu36I | CCTNAGG | 1 cut(s) | 415 |
| BsuRI | GGCC | 5 cut(s) | 5, 633, 1056, 1142, 1272 |
| BtgI | CCRYGG | 1 cut(s) | 904 |
| BtsCI | GGATG | 4 cut(s) | 388, 564, 724, 760 |
| BtsIMutI | CAGTG | 1 cut(s) | 1242 |
| BveI | ACCTGC | 3 cut(s) | 190, 320, 1293 |
| Cac8I | GCNNGC | 5 cut(s) | 349, 353, 542, 624, 1107 |
| CciI | TCATGA | 1 cut(s) | 552 |
| CfoI | GCGC | 2 cut(s) | 276, 1111 |
| Cfr13I | GGNCC | 2 cut(s) | 578, 1141 |
| CseI | GACGC | 1 cut(s) | 491 |
| Csp6I | GTAC | 5 cut(s) | 59, 175, 410, 819, 1189 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 124, 159 |
| CviAII | CATG | 8 cut(s) | 482, 553, 623, 661, 905, 1091, 1121, 1192 |
| CviQI | GTAC | 5 cut(s) | 59, 175, 410, 819, 1189 |
| DdeI | CTNAG | 7 cut(s) | 314, 415, 849, 1137, 1341, 1363, 1451 |
| DpnI | GATC | 5 cut(s) | 375, 477, 588, 912, 1182 |
| DpnII | GATC | 5 cut(s) | 373, 475, 586, 910, 1180 |
| DraI | TTTAAA | 1 cut(s) | 775 |
| DriI | GACNNNNNGTC | 1 cut(s) | 177 |
| EaeI | YGGCCR | 4 cut(s) | 3, 631, 1054, 1270 |
| Eam1104I | CTCTTC | 4 cut(s) | 29, 213, 915, 1341 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 177 |
| EarI | CTCTTC | 4 cut(s) | 29, 213, 915, 1341 |
| EciI | GGCGGA | 1 cut(s) | 381 |
| Ecl136II | GAGCTC | 2 cut(s) | 925, 1340 |
| Eco105I | TACGTA | 1 cut(s) | 1188 |
| Eco130I | CCWWGG | 5 cut(s) | 519, 882, 904, 995, 1267 |
| Eco24I | GRGCYC | 3 cut(s) | 105, 927, 1342 |
| Eco47I | GGWCC | 1 cut(s) | 578 |
| Eco53kI | GAGCTC | 2 cut(s) | 925, 1340 |
| Eco57I | CTGAAG | 2 cut(s) | 688, 744 |
| Eco81I | CCTNAGG | 1 cut(s) | 415 |
| Eco88I | CYCGRG | 1 cut(s) | 104 |
| EcoICRI | GAGCTC | 2 cut(s) | 925, 1340 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1271 |
| EcoRI | GAATTC | 1 cut(s) | 853 |
| EcoRII | CCWGG | 3 cut(s) | 466, 1211, 1272 |
| EcoT14I | CCWWGG | 5 cut(s) | 519, 882, 904, 995, 1267 |
| EcoT22I | ATGCAT | 1 cut(s) | 1030 |
| EcoT38I | GRGCYC | 3 cut(s) | 105, 927, 1342 |
| ErhI | CCWWGG | 5 cut(s) | 519, 882, 904, 995, 1267 |
| FaeI | CATG | 8 cut(s) | 485, 556, 626, 664, 908, 1094, 1124, 1195 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1362, 1394 |
| FaqI | GGGAC | 1 cut(s) | 1138 |
| FatI | CATG | 8 cut(s) | 481, 552, 622, 660, 904, 1090, 1120, 1191 |
| FauNDI | CATATG | 1 cut(s) | 733 |
| FbaI | TGATCA | 1 cut(s) | 1180 |
| FblI | GTMKAC | 2 cut(s) | 797, 1012 |
| Fnu4HI | GCNGC | 4 cut(s) | 239, 327, 1300, 1407 |
| FokI | GGATG | 4 cut(s) | 375, 571, 731, 747 |
| FriOI | GRGCYC | 3 cut(s) | 105, 927, 1342 |
| Fsp4HI | GCNGC | 4 cut(s) | 239, 327, 1300, 1407 |
| FspBI | CTAG | 3 cut(s) | 260, 972, 1335 |
| GlaI | GCGC | 2 cut(s) | 275, 1110 |
| GluI | GCNGC | 4 cut(s) | 239, 327, 1300, 1407 |
| GsaI | CCCAGC | 1 cut(s) | 454 |
| HaeIII | GGCC | 5 cut(s) | 5, 633, 1056, 1142, 1272 |
| HapII | CCGG | 3 cut(s) | 227, 862, 1427 |
| HgaI | GACGC | 1 cut(s) | 491 |
| HhaI | GCGC | 2 cut(s) | 276, 1111 |
| Hin1II | CATG | 8 cut(s) | 485, 556, 626, 664, 908, 1094, 1124, 1195 |
| Hin6I | GCGC | 2 cut(s) | 274, 1109 |
| HinP1I | GCGC | 2 cut(s) | 274, 1109 |
| HincII | GTYRAC | 2 cut(s) | 1279, 1360 |
| HindII | GTYRAC | 2 cut(s) | 1279, 1360 |
| HindIII | AAGCTT | 2 cut(s) | 809, 1421 |
| HpaII | CCGG | 3 cut(s) | 227, 862, 1427 |
| HphI | GGTGA | 4 cut(s) | 347, 898, 1024, 1256 |
| Hpy166II | GTNNAC | 6 cut(s) | 798, 819, 821, 1013, 1279, 1360 |
| Hpy8I | GTNNAC | 6 cut(s) | 798, 819, 821, 1013, 1279, 1360 |
| HpyAV | CCTTC | 5 cut(s) | 557, 849, 941, 1235, 1346 |
| HpyCH4III | ACNGT | 3 cut(s) | 179, 409, 1246 |
| HpyCH4IV | ACGT | 3 cut(s) | 173, 1009, 1187 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 757, 878 |
| HpyF3I | CTNAG | 7 cut(s) | 314, 415, 849, 1137, 1341, 1363, 1451 |
| HpySE526I | ACGT | 3 cut(s) | 173, 1009, 1187 |
| Hsp92II | CATG | 8 cut(s) | 485, 556, 626, 664, 908, 1094, 1124, 1195 |
| HspAI | GCGC | 2 cut(s) | 274, 1109 |
| Kpn2I | TCCGGA | 2 cut(s) | 226, 1426 |
| Ksp22I | TGATCA | 1 cut(s) | 1180 |
| Kzo9I | GATC | 5 cut(s) | 373, 475, 586, 910, 1180 |
| LguI | GCTCTTC | 1 cut(s) | 915 |
| LmnI | GCTCC | 4 cut(s) | 189, 229, 515, 930 |
| Lsp1109I | GCAGC | 4 cut(s) | 225, 313, 1286, 1418 |
| LweI | GCATC | 3 cut(s) | 709, 769, 951 |
| MaeI | CTAG | 3 cut(s) | 260, 972, 1335 |
| MaeII | ACGT | 3 cut(s) | 173, 1009, 1187 |
| MaeIII | GTNAC | 1 cut(s) | 169 |
| MalI | GATC | 5 cut(s) | 375, 477, 588, 912, 1182 |
| MbiI | CCGCTC | 1 cut(s) | 751 |
| MboI | GATC | 5 cut(s) | 373, 475, 586, 910, 1180 |
| MfeI | CAATTG | 1 cut(s) | 1176 |
| MflI | RGATCY | 1 cut(s) | 586 |
| MhlI | GDGCHC | 3 cut(s) | 105, 927, 1342 |
| MlsI | TGGCCA | 3 cut(s) | 5, 1056, 1272 |
| MluNI | TGGCCA | 3 cut(s) | 5, 1056, 1272 |
| MlyI | GAGTC | 3 cut(s) | 500, 1120, 1355 |
| Mox20I | TGGCCA | 3 cut(s) | 5, 1056, 1272 |
| Mph1103I | ATGCAT | 1 cut(s) | 1030 |
| MroI | TCCGGA | 2 cut(s) | 226, 1426 |
| MroXI | GAANNNNTTC | 2 cut(s) | 38, 701 |
| MscI | TGGCCA | 3 cut(s) | 5, 1056, 1272 |
| MseI | TTAA | 5 cut(s) | 774, 789, 990, 1328, 1456 |
| MslI | CAYNNNNRTG | 2 cut(s) | 557, 1247 |
| Msp20I | TGGCCA | 3 cut(s) | 5, 1056, 1272 |
| MspA1I | CMGCKG | 1 cut(s) | 540 |
| MspI | CCGG | 3 cut(s) | 227, 862, 1427 |
| MspR9I | CCNGG | 4 cut(s) | 468, 862, 1213, 1274 |
| MunI | CAATTG | 1 cut(s) | 1176 |
| Mva1269I | GAATGC | 1 cut(s) | 1028 |
| MvaI | CCWGG | 3 cut(s) | 468, 1213, 1274 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 757, 878 |
| NciI | CCSGG | 1 cut(s) | 862 |
| NcoI | CCATGG | 1 cut(s) | 904 |
| NdeI | CATATG | 1 cut(s) | 733 |
| NdeII | GATC | 5 cut(s) | 373, 475, 586, 910, 1180 |
| NlaIII | CATG | 8 cut(s) | 485, 556, 626, 664, 908, 1094, 1124, 1195 |
| NlaIV | GGNNCC | 5 cut(s) | 225, 517, 545, 588, 1209 |
| NmuCI | GTSAC | 1 cut(s) | 169 |
| NsiI | ATGCAT | 1 cut(s) | 1030 |
| NspI | RCATGY | 2 cut(s) | 485, 626 |
| PaeI | GCATGC | 1 cut(s) | 626 |
| PagI | TCATGA | 1 cut(s) | 552 |
| PaqCI | CACCTGC | 1 cut(s) | 320 |
| PciSI | GCTCTTC | 1 cut(s) | 915 |
| PctI | GAATGC | 1 cut(s) | 1028 |
| PdmI | GAANNNNTTC | 2 cut(s) | 38, 701 |
| PfeI | GAWTC | 7 cut(s) | 46, 65, 71, 301, 463, 565, 1149 |
| PfoI | TCCNGGA | 1 cut(s) | 466 |
| PkrI | GCNGC | 4 cut(s) | 240, 328, 1301, 1408 |
| PleI | GAGTC | 3 cut(s) | 499, 1120, 1355 |
| PpsI | GAGTC | 3 cut(s) | 499, 1120, 1355 |
| Ppu21I | YACGTR | 2 cut(s) | 1010, 1188 |
| PsiI | TTATAA | 1 cut(s) | 896 |
| Psp124BI | GAGCTC | 2 cut(s) | 927, 1342 |
| Psp6I | CCWGG | 3 cut(s) | 466, 1211, 1272 |
| PspFI | CCCAGC | 1 cut(s) | 450 |
| PspGI | CCWGG | 3 cut(s) | 466, 1211, 1272 |
| PspN4I | GGNNCC | 5 cut(s) | 225, 517, 545, 588, 1209 |
| PspPI | GGNCC | 2 cut(s) | 578, 1141 |
| PstI | CTGCAG | 2 cut(s) | 331, 1304 |
| PsuI | RGATCY | 1 cut(s) | 586 |
| PvuII | CAGCTG | 1 cut(s) | 540 |
| RsaI | GTAC | 5 cut(s) | 60, 176, 411, 820, 1190 |
| RsaNI | GTAC | 5 cut(s) | 59, 175, 410, 819, 1189 |
| RseI | CAYNNNNRTG | 2 cut(s) | 557, 1247 |
| SacI | GAGCTC | 2 cut(s) | 927, 1342 |
| SapI | GCTCTTC | 1 cut(s) | 915 |
| SaqAI | TTAA | 5 cut(s) | 774, 789, 990, 1328, 1456 |
| SatI | GCNGC | 4 cut(s) | 239, 327, 1300, 1407 |
| Sau3AI | GATC | 5 cut(s) | 373, 475, 586, 910, 1180 |
| Sau96I | GGNCC | 2 cut(s) | 578, 1141 |
| SchI | GAGTC | 3 cut(s) | 500, 1120, 1355 |
| ScrFI | CCNGG | 4 cut(s) | 468, 862, 1213, 1274 |
| SduI | GDGCHC | 3 cut(s) | 105, 927, 1342 |
| SfaNI | GCATC | 3 cut(s) | 709, 769, 951 |
| SfcI | CTRYAG | 2 cut(s) | 327, 1300 |
| SinI | GGWCC | 1 cut(s) | 578 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 557, 1247 |
| SmlI | CTYRAG | 2 cut(s) | 269, 602 |
| SmoI | CTYRAG | 2 cut(s) | 269, 602 |
| SnaBI | TACGTA | 1 cut(s) | 1188 |
| SphI | GCATGC | 1 cut(s) | 626 |
| SsiI | CCGC | 2 cut(s) | 392, 751 |
| SspMI | CTAG | 3 cut(s) | 260, 972, 1335 |
| SstI | GAGCTC | 2 cut(s) | 927, 1342 |
| StyD4I | CCNGG | 4 cut(s) | 466, 860, 1211, 1272 |
| StyI | CCWWGG | 5 cut(s) | 519, 882, 904, 995, 1267 |
| TaaI | ACNGT | 3 cut(s) | 179, 409, 1246 |
| TaiI | ACGT | 3 cut(s) | 176, 1012, 1190 |
| TaqI | TCGA | 1 cut(s) | 950 |
| TaqII | GACCGA | 1 cut(s) | 9 |
| TatI | WGTACW | 2 cut(s) | 58, 818 |
| TfiI | GAWTC | 7 cut(s) | 46, 65, 71, 301, 463, 565, 1149 |
| Tru1I | TTAA | 5 cut(s) | 774, 789, 990, 1328, 1456 |
| Tru9I | TTAA | 5 cut(s) | 774, 789, 990, 1328, 1456 |
| TscAI | CASTG | 1 cut(s) | 1249 |
| TseFI | GTSAC | 1 cut(s) | 169 |
| TseI | GCWGC | 4 cut(s) | 238, 326, 1299, 1406 |
| Tsp45I | GTSAC | 1 cut(s) | 169 |
| TspDTI | ATGAA | 4 cut(s) | 542, 575, 731, 1383 |
| TspGWI | ACGGA | 1 cut(s) | 1030 |
| TspRI | CASTG | 1 cut(s) | 1249 |
| VpaK11BI | GGWCC | 1 cut(s) | 578 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1271 |
| XapI | RAATTY | 4 cut(s) | 246, 287, 853, 1154 |
| XbaI | TCTAGA | 1 cut(s) | 259 |
| XceI | RCATGY | 2 cut(s) | 485, 626 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 680 |
| XmiI | GTMKAC | 2 cut(s) | 797, 1012 |
| XmnI | GAANNNNTTC | 2 cut(s) | 38, 701 |
| XspI | CTAG | 3 cut(s) | 260, 972, 1335 |
| Zsp2I | ATGCAT | 1 cut(s) | 1030 |