Belongs to the AAA ATPase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 459 |
| AciI | CCGC | 1 cut(s) | 786 |
| AclWI | GGATC | 1 cut(s) | 830 |
| AcsI | RAATTY | 4 cut(s) | 183, 359, 931, 1172 |
| AfaI | GTAC | 2 cut(s) | 170, 719 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 47, 153, 331, 587, 974 |
| AgsI | TTSAA | 8 cut(s) | 137, 907, 931, 965, 1057, 1070, 1177, 1268 |
| AjnI | CCWGG | 2 cut(s) | 389, 772 |
| AluBI | AGCT | 8 cut(s) | 249, 376, 463, 565, 612, 998, 1073, 1235 |
| AluI | AGCT | 8 cut(s) | 249, 376, 463, 565, 612, 998, 1073, 1235 |
| Alw21I | GWGCWC | 2 cut(s) | 403, 614 |
| Alw26I | GTCTC | 3 cut(s) | 321, 445, 974 |
| Alw44I | GTGCAC | 1 cut(s) | 399 |
| AlwI | GGATC | 1 cut(s) | 830 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 779 |
| AoxI | GGCC | 2 cut(s) | 38, 767 |
| ApaLI | GTGCAC | 1 cut(s) | 399 |
| ApoI | RAATTY | 4 cut(s) | 183, 359, 931, 1172 |
| AspA2I | CCTAGG | 1 cut(s) | 404 |
| AspLEI | GCGC | 1 cut(s) | 222 |
| AspS9I | GGNCC | 1 cut(s) | 767 |
| AsuHPI | GGTGA | 4 cut(s) | 92, 361, 620, 803 |
| AvrII | CCTAGG | 1 cut(s) | 404 |
| BaeGI | GKGCMC | 1 cut(s) | 403 |
| BanII | GRGCYC | 3 cut(s) | 53, 62, 614 |
| Bbv12I | GWGCWC | 2 cut(s) | 403, 614 |
| BbvCI | CCTCAGC | 2 cut(s) | 254, 1110 |
| BccI | CCATC | 3 cut(s) | 248, 856, 1052 |
| BceAI | ACGGC | 1 cut(s) | 299 |
| BciT130I | CCWGG | 2 cut(s) | 391, 774 |
| BciVI | GTATCC | 1 cut(s) | 584 |
| BclI | TGATCA | 1 cut(s) | 625 |
| BcoDI | GTCTC | 3 cut(s) | 321, 445, 974 |
| BfaI | CTAG | 3 cut(s) | 320, 405, 1076 |
| BfmI | CTRYAG | 2 cut(s) | 270, 615 |
| BfuI | GTATCC | 1 cut(s) | 584 |
| BlnI | CCTAGG | 1 cut(s) | 404 |
| BmcAI | AGTACT | 1 cut(s) | 719 |
| Bme1390I | CCNGG | 2 cut(s) | 391, 774 |
| BmgT120I | GGNCC | 1 cut(s) | 767 |
| BmiI | GGNNCC | 2 cut(s) | 128, 660 |
| BmrFI | CCNGG | 2 cut(s) | 391, 774 |
| BmrI | ACTGGG | 2 cut(s) | 56, 65 |
| BmsI | GCATC | 3 cut(s) | 1008, 1030, 1132 |
| BmuI | ACTGGG | 2 cut(s) | 56, 65 |
| BpmI | CTGGAG | 1 cut(s) | 978 |
| Bpu10I | CCTNAGC | 2 cut(s) | 254, 1110 |
| BsaI | GGTCTC | 1 cut(s) | 974 |
| BsaJI | CCNNGG | 3 cut(s) | 364, 404, 740 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 47, 153, 331, 587, 974 |
| Bse118I | RCCGGY | 1 cut(s) | 203 |
| Bse1I | ACTGG | 2 cut(s) | 51, 60 |
| Bse3DI | GCAATG | 1 cut(s) | 660 |
| BseBI | CCWGG | 2 cut(s) | 391, 774 |
| BseDI | CCNNGG | 3 cut(s) | 364, 404, 740 |
| BseGI | GGATG | 7 cut(s) | 18, 177, 240, 340, 867, 1045, 1147 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 47, 153, 331, 587, 974 |
| BseMI | GCAATG | 1 cut(s) | 660 |
| BseMII | CTCAG | 3 cut(s) | 75, 268, 1101 |
| BseNI | ACTGG | 2 cut(s) | 51, 60 |
| BseRI | GAGGAG | 2 cut(s) | 1106, 1208 |
| BseSI | GKGCMC | 1 cut(s) | 403 |
| BsgI | GTGCAG | 1 cut(s) | 825 |
| BshFI | GGCC | 2 cut(s) | 40, 769 |
| BsiHKAI | GWGCWC | 2 cut(s) | 403, 614 |
| BsiSI | CCGG | 1 cut(s) | 204 |
| BslFI | GGGAC | 1 cut(s) | 500 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 47, 153, 331, 587, 974 |
| BsmAI | GTCTC | 3 cut(s) | 321, 445, 974 |
| BsmBI | CGTCTC | 1 cut(s) | 445 |
| BsmFI | GGGAC | 1 cut(s) | 500 |
| BsmI | GAATGC | 1 cut(s) | 902 |
| BsnI | GGCC | 2 cut(s) | 40, 769 |
| Bso31I | GGTCTC | 1 cut(s) | 974 |
| Bsp1286I | GDGCHC | 4 cut(s) | 53, 62, 403, 614 |
| Bsp143I | GATC | 2 cut(s) | 625, 835 |
| BspACI | CCGC | 1 cut(s) | 786 |
| BspANI | GGCC | 2 cut(s) | 40, 769 |
| BspCNI | CTCAG | 3 cut(s) | 74, 267, 1102 |
| BspLI | GGNNCC | 2 cut(s) | 128, 660 |
| BspPI | GGATC | 1 cut(s) | 830 |
| BspTNI | GGTCTC | 1 cut(s) | 974 |
| BsrDI | GCAATG | 1 cut(s) | 660 |
| BsrFI | RCCGGY | 1 cut(s) | 203 |
| BsrI | ACTGG | 2 cut(s) | 51, 60 |
| BssAI | RCCGGY | 1 cut(s) | 203 |
| BssECI | CCNNGG | 3 cut(s) | 364, 404, 740 |
| BssMI | GATC | 2 cut(s) | 625, 835 |
| BssT1I | CCWWGG | 3 cut(s) | 364, 404, 740 |
| Bst2UI | CCWGG | 2 cut(s) | 391, 774 |
| Bst6I | CTCTTC | 3 cut(s) | 339, 443, 1206 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 783 |
| BstC8I | GCNNGC | 5 cut(s) | 69, 205, 784, 915, 1123 |
| BstDEI | CTNAG | 3 cut(s) | 61, 254, 1110 |
| BstF5I | GGATG | 7 cut(s) | 18, 177, 240, 340, 867, 1045, 1147 |
| BstHHI | GCGC | 1 cut(s) | 222 |
| BstKTI | GATC | 2 cut(s) | 628, 838 |
| BstMAI | GTCTC | 3 cut(s) | 321, 445, 974 |
| BstMBI | GATC | 2 cut(s) | 625, 835 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 57, 255, 562, 783, 1004 |
| BstNI | CCWGG | 2 cut(s) | 391, 774 |
| BstSCI | CCNGG | 2 cut(s) | 389, 772 |
| BstSFI | CTRYAG | 2 cut(s) | 270, 615 |
| BstSLI | GKGCMC | 1 cut(s) | 403 |
| BsuI | GTATCC | 1 cut(s) | 584 |
| BsuRI | GGCC | 2 cut(s) | 40, 769 |
| BtsCI | GGATG | 7 cut(s) | 18, 177, 240, 340, 867, 1045, 1147 |
| BtsI | GCAGTG | 3 cut(s) | 229, 549, 777 |
| BtsIMutI | CAGTG | 6 cut(s) | 53, 69, 229, 549, 777, 842 |
| Cac8I | GCNNGC | 5 cut(s) | 69, 205, 784, 915, 1123 |
| CaiI | CAGNNNCTG | 1 cut(s) | 779 |
| CfoI | GCGC | 1 cut(s) | 222 |
| Cfr10I | RCCGGY | 1 cut(s) | 203 |
| Cfr13I | GGNCC | 1 cut(s) | 767 |
| Csp6I | GTAC | 2 cut(s) | 169, 718 |
| CviAII | CATG | 6 cut(s) | 94, 331, 515, 694, 944, 988 |
| CviQI | GTAC | 2 cut(s) | 169, 718 |
| DdeI | CTNAG | 3 cut(s) | 61, 254, 1110 |
| DpnI | GATC | 2 cut(s) | 627, 837 |
| DpnII | GATC | 2 cut(s) | 625, 835 |
| Eam1104I | CTCTTC | 3 cut(s) | 339, 443, 1206 |
| EarI | CTCTTC | 3 cut(s) | 339, 443, 1206 |
| Ecl136II | GAGCTC | 1 cut(s) | 612 |
| Eco130I | CCWWGG | 3 cut(s) | 364, 404, 740 |
| Eco24I | GRGCYC | 3 cut(s) | 53, 62, 614 |
| Eco31I | GGTCTC | 1 cut(s) | 974 |
| Eco53kI | GAGCTC | 1 cut(s) | 612 |
| EcoICRI | GAGCTC | 1 cut(s) | 612 |
| EcoRI | GAATTC | 1 cut(s) | 359 |
| EcoRII | CCWGG | 2 cut(s) | 389, 772 |
| EcoT14I | CCWWGG | 3 cut(s) | 364, 404, 740 |
| EcoT38I | GRGCYC | 3 cut(s) | 53, 62, 614 |
| ErhI | CCWWGG | 3 cut(s) | 364, 404, 740 |
| Esp3I | CGTCTC | 1 cut(s) | 445 |
| FaeI | CATG | 6 cut(s) | 97, 334, 518, 697, 947, 991 |
| FaqI | GGGAC | 1 cut(s) | 500 |
| FatI | CATG | 6 cut(s) | 93, 330, 514, 693, 943, 987 |
| FauI | CCCGC | 1 cut(s) | 779 |
| FbaI | TGATCA | 1 cut(s) | 625 |
| FokI | GGATG | 7 cut(s) | 25, 164, 227, 347, 874, 1052, 1154 |
| FriOI | GRGCYC | 3 cut(s) | 53, 62, 614 |
| FspBI | CTAG | 3 cut(s) | 320, 405, 1076 |
| GlaI | GCGC | 1 cut(s) | 221 |
| GsuI | CTGGAG | 1 cut(s) | 978 |
| HaeIII | GGCC | 2 cut(s) | 40, 769 |
| HapII | CCGG | 1 cut(s) | 204 |
| HhaI | GCGC | 1 cut(s) | 222 |
| Hin1II | CATG | 6 cut(s) | 97, 334, 518, 697, 947, 991 |
| Hin6I | GCGC | 1 cut(s) | 220 |
| HinP1I | GCGC | 1 cut(s) | 220 |
| HindIII | AAGCTT | 1 cut(s) | 563 |
| HinfI | GANTC | 5 cut(s) | 547, 798, 938, 991, 1196 |
| HpaII | CCGG | 1 cut(s) | 204 |
| HphI | GGTGA | 4 cut(s) | 92, 361, 620, 803 |
| Hpy166II | GTNNAC | 5 cut(s) | 169, 234, 401, 810, 1153 |
| Hpy188I | TCNGA | 4 cut(s) | 126, 442, 956, 1226 |
| Hpy188III | TCNNGA | 5 cut(s) | 7, 509, 588, 733, 1076 |
| Hpy8I | GTNNAC | 5 cut(s) | 169, 234, 401, 810, 1153 |
| HpyAV | CCTTC | 6 cut(s) | 521, 526, 706, 852, 1099, 1123 |
| HpyCH4IV | ACGT | 1 cut(s) | 454 |
| HpyCH4V | TGCA | 8 cut(s) | 214, 401, 635, 725, 842, 917, 1085, 1145 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 57, 255, 562, 783, 1004 |
| HpyF3I | CTNAG | 3 cut(s) | 61, 254, 1110 |
| HpySE526I | ACGT | 1 cut(s) | 454 |
| Hsp92II | CATG | 6 cut(s) | 97, 334, 518, 697, 947, 991 |
| HspAI | GCGC | 1 cut(s) | 220 |
| KroI | GCCGGC | 1 cut(s) | 203 |
| KroNI | GCCGGC | 1 cut(s) | 205 |
| Ksp22I | TGATCA | 1 cut(s) | 625 |
| Kzo9I | GATC | 2 cut(s) | 625, 835 |
| LmnI | GCTCC | 3 cut(s) | 126, 609, 658 |
| LweI | GCATC | 3 cut(s) | 1008, 1030, 1132 |
| MaeI | CTAG | 3 cut(s) | 320, 405, 1076 |
| MaeII | ACGT | 1 cut(s) | 454 |
| MaeIII | GTNAC | 1 cut(s) | 349 |
| MalI | GATC | 2 cut(s) | 627, 837 |
| MboI | GATC | 2 cut(s) | 625, 835 |
| MboII | GAAGA | 7 cut(s) | 356, 405, 460, 749, 1069, 1127, 1223 |
| MhlI | GDGCHC | 4 cut(s) | 53, 62, 403, 614 |
| MluCI | AATT | 8 cut(s) | 183, 359, 817, 878, 931, 947, 1061, 1172 |
| MlyI | GAGTC | 3 cut(s) | 556, 985, 1205 |
| MroNI | GCCGGC | 1 cut(s) | 203 |
| MseI | TTAA | 4 cut(s) | 149, 372, 573, 1137 |
| MslI | CAYNNNNRTG | 1 cut(s) | 653 |
| MspA1I | CMGCKG | 1 cut(s) | 998 |
| MspI | CCGG | 1 cut(s) | 204 |
| MspR9I | CCNGG | 2 cut(s) | 391, 774 |
| Mva1269I | GAATGC | 1 cut(s) | 902 |
| MvaI | CCWGG | 2 cut(s) | 391, 774 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 57, 255, 562, 783, 1004 |
| NaeI | GCCGGC | 1 cut(s) | 205 |
| NdeII | GATC | 2 cut(s) | 625, 835 |
| NgoMIV | GCCGGC | 1 cut(s) | 203 |
| NlaIII | CATG | 6 cut(s) | 97, 334, 518, 697, 947, 991 |
| NlaIV | GGNNCC | 2 cut(s) | 128, 660 |
| NmuCI | GTSAC | 1 cut(s) | 349 |
| PctI | GAATGC | 1 cut(s) | 902 |
| PdiI | GCCGGC | 1 cut(s) | 205 |
| PfeI | GAWTC | 2 cut(s) | 798, 938 |
| PfoI | TCCNGGA | 1 cut(s) | 389 |
| PleI | GAGTC | 3 cut(s) | 555, 985, 1204 |
| PpsI | GAGTC | 3 cut(s) | 555, 985, 1204 |
| PsiI | TTATAA | 1 cut(s) | 459 |
| Psp124BI | GAGCTC | 1 cut(s) | 614 |
| Psp6I | CCWGG | 2 cut(s) | 389, 772 |
| PspGI | CCWGG | 2 cut(s) | 389, 772 |
| PspN4I | GGNNCC | 2 cut(s) | 128, 660 |
| PspPI | GGNCC | 1 cut(s) | 767 |
| PstNI | CAGNNNCTG | 1 cut(s) | 779 |
| PvuII | CAGCTG | 1 cut(s) | 998 |
| RsaI | GTAC | 2 cut(s) | 170, 719 |
| RsaNI | GTAC | 2 cut(s) | 169, 718 |
| RseI | CAYNNNNRTG | 1 cut(s) | 653 |
| SacI | GAGCTC | 1 cut(s) | 614 |
| SaqAI | TTAA | 4 cut(s) | 149, 372, 573, 1137 |
| Sau3AI | GATC | 2 cut(s) | 625, 835 |
| Sau96I | GGNCC | 1 cut(s) | 767 |
| ScaI | AGTACT | 1 cut(s) | 719 |
| SchI | GAGTC | 3 cut(s) | 556, 985, 1205 |
| ScrFI | CCNGG | 2 cut(s) | 391, 774 |
| SduI | GDGCHC | 4 cut(s) | 53, 62, 403, 614 |
| SfaNI | GCATC | 3 cut(s) | 1008, 1030, 1132 |
| SfcI | CTRYAG | 2 cut(s) | 270, 615 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 653 |
| SmlI | CTYRAG | 1 cut(s) | 5 |
| SmoI | CTYRAG | 1 cut(s) | 5 |
| Sse9I | AATT | 8 cut(s) | 183, 359, 817, 878, 931, 947, 1061, 1172 |
| SsiI | CCGC | 1 cut(s) | 786 |
| SspMI | CTAG | 3 cut(s) | 320, 405, 1076 |
| SstI | GAGCTC | 1 cut(s) | 614 |
| StyD4I | CCNGG | 2 cut(s) | 389, 772 |
| StyI | CCWWGG | 3 cut(s) | 364, 404, 740 |
| TaiI | ACGT | 1 cut(s) | 457 |
| TaqII | GACCGA | 1 cut(s) | 426 |
| TasI | AATT | 8 cut(s) | 183, 359, 817, 878, 931, 947, 1061, 1172 |
| TatI | WGTACW | 1 cut(s) | 717 |
| TfiI | GAWTC | 2 cut(s) | 798, 938 |
| Tru1I | TTAA | 4 cut(s) | 149, 372, 573, 1137 |
| Tru9I | TTAA | 4 cut(s) | 149, 372, 573, 1137 |
| TscAI | CASTG | 6 cut(s) | 60, 69, 229, 549, 784, 849 |
| TseFI | GTSAC | 1 cut(s) | 349 |
| Tsp45I | GTSAC | 1 cut(s) | 349 |
| TspDTI | ATGAA | 7 cut(s) | 210, 357, 372, 405, 912, 960, 1224 |
| TspRI | CASTG | 6 cut(s) | 60, 69, 229, 549, 784, 849 |
| VneI | GTGCAC | 1 cut(s) | 399 |
| XapI | RAATTY | 4 cut(s) | 183, 359, 931, 1172 |
| XbaI | TCTAGA | 1 cut(s) | 1075 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 820 |
| XmaJI | CCTAGG | 1 cut(s) | 404 |
| XspI | CTAG | 3 cut(s) | 320, 405, 1076 |
| ZrmI | AGTACT | 1 cut(s) | 719 |