mitochondrial chaperone
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 1022 |
| Acc16I | TGCGCA | 1 cut(s) | 371 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 178 |
| AccBSI | CCGCTC | 1 cut(s) | 361 |
| AccII | CGCG | 3 cut(s) | 853, 977, 1230 |
| AccIII | TCCGGA | 1 cut(s) | 1396 |
| AclWI | GGATC | 4 cut(s) | 204, 217, 1121, 1134 |
| AcoI | YGGCCR | 1 cut(s) | 933 |
| AcsI | RAATTY | 3 cut(s) | 67, 99, 1198 |
| AcuI | CTGAAG | 1 cut(s) | 686 |
| AcyI | GRCGYC | 2 cut(s) | 533, 1019 |
| AfaI | GTAC | 6 cut(s) | 96, 257, 293, 545, 572, 599 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 178, 360, 670, 905, 999, 1403 |
| AgsI | TTSAA | 8 cut(s) | 51, 343, 451, 511, 946, 1319, 1348, 1546 |
| AjnI | CCWGG | 1 cut(s) | 1173 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 165, 197 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 491, 523 |
| Alw21I | GWGCWC | 3 cut(s) | 124, 410, 756 |
| Alw26I | GTCTC | 2 cut(s) | 764, 1010 |
| AlwI | GGATC | 4 cut(s) | 204, 217, 1121, 1134 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1504 |
| Ama87I | CYCGRG | 1 cut(s) | 898 |
| Aor13HI | TCCGGA | 1 cut(s) | 1396 |
| AoxI | GGCC | 3 cut(s) | 678, 892, 933 |
| ApeKI | GCWGC | 6 cut(s) | 372, 556, 1006, 1192, 1280, 1586 |
| ApoI | RAATTY | 3 cut(s) | 67, 99, 1198 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 710, 742 |
| AspLEI | GCGC | 3 cut(s) | 372, 435, 853 |
| AspS9I | GGNCC | 3 cut(s) | 631, 644, 893 |
| AsuC2I | CCSGG | 6 cut(s) | 355, 538, 899, 900, 1189, 1477 |
| AsuHPI | GGTGA | 3 cut(s) | 343, 592, 1611 |
| AsuII | TTCGAA | 1 cut(s) | 274 |
| AvaI | CYCGRG | 1 cut(s) | 898 |
| AvaII | GGWCC | 2 cut(s) | 631, 644 |
| BalI | TGGCCA | 1 cut(s) | 935 |
| BamHI | GGATCC | 2 cut(s) | 209, 1126 |
| BanII | GRGCYC | 2 cut(s) | 170, 410 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 863, 895 |
| BauI | CACGAG | 1 cut(s) | 488 |
| Bbv12I | GWGCWC | 3 cut(s) | 124, 410, 756 |
| BbvCI | CCTCAGC | 3 cut(s) | 837, 998, 1551 |
| BbvI | GCAGC | 6 cut(s) | 384, 568, 993, 1179, 1267, 1573 |
| BccI | CCATC | 6 cut(s) | 398, 814, 1135, 1297, 1588, 1630 |
| BciT130I | CCWGG | 1 cut(s) | 1175 |
| BciVI | GTATCC | 2 cut(s) | 330, 1603 |
| BcnI | CCSGG | 6 cut(s) | 355, 538, 899, 900, 1189, 1477 |
| BcoDI | GTCTC | 2 cut(s) | 764, 1010 |
| BfaI | CTAG | 2 cut(s) | 113, 1359 |
| BfmI | CTRYAG | 2 cut(s) | 1496, 1587 |
| BfoI | RGCGCY | 1 cut(s) | 436 |
| BfuI | GTATCC | 2 cut(s) | 330, 1603 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 441, 932 |
| BisI | GCNGC | 9 cut(s) | 362, 373, 557, 927, 1007, 1193, 1228, 1281, 1587 |
| BlpI | GCTNAGC | 1 cut(s) | 552 |
| BlsI | GCNGC | 9 cut(s) | 363, 374, 558, 928, 1008, 1194, 1229, 1282, 1588 |
| BmcAI | AGTACT | 2 cut(s) | 257, 293 |
| Bme1390I | CCNGG | 7 cut(s) | 355, 538, 899, 900, 1175, 1189, 1477 |
| Bme18I | GGWCC | 2 cut(s) | 631, 644 |
| BmeT110I | CYCGRG | 1 cut(s) | 898 |
| BmgT120I | GGNCC | 3 cut(s) | 631, 644, 893 |
| BmiI | GGNNCC | 7 cut(s) | 183, 211, 632, 646, 737, 895, 1128 |
| BmrFI | CCNGG | 7 cut(s) | 355, 538, 899, 900, 1175, 1189, 1477 |
| BmrI | ACTGGG | 1 cut(s) | 915 |
| BmsI | GCATC | 4 cut(s) | 13, 357, 1267, 1449 |
| BmuI | ACTGGG | 1 cut(s) | 915 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 368, 400, 1224, 1256 |
| BpmI | CTGGAG | 3 cut(s) | 754, 1419, 1502 |
| Bpu10I | CCTNAGC | 5 cut(s) | 404, 653, 837, 998, 1551 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 552 |
| Bpu14I | TTCGAA | 1 cut(s) | 274 |
| BpuEI | CTTGAG | 3 cut(s) | 422, 986, 1355 |
| BpuMI | CCSGG | 6 cut(s) | 355, 538, 899, 900, 1189, 1477 |
| Bsa29I | ATCGAT | 1 cut(s) | 1053 |
| BsaAI | YACGTR | 1 cut(s) | 1264 |
| BsaHI | GRCGYC | 2 cut(s) | 533, 1019 |
| BsaJI | CCNNGG | 9 cut(s) | 205, 354, 897, 898, 899, 930, 975, 1228, 1476 |
| BsaWI | WCCGGW | 2 cut(s) | 577, 1396 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 392, 422, 507, 537 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 178, 360, 670, 905, 999, 1403 |
| Bse118I | RCCGGY | 1 cut(s) | 696 |
| Bse1I | ACTGG | 3 cut(s) | 460, 737, 910 |
| Bse3DI | GCAATG | 1 cut(s) | 1409 |
| BseAI | TCCGGA | 1 cut(s) | 1396 |
| BseBI | CCWGG | 1 cut(s) | 1175 |
| BseCI | ATCGAT | 1 cut(s) | 1053 |
| BseDI | CCNNGG | 9 cut(s) | 205, 354, 897, 898, 899, 930, 975, 1228, 1476 |
| BseGI | GGATG | 4 cut(s) | 4, 505, 1308, 1486 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 178, 360, 670, 905, 999, 1403 |
| BseMI | GCAATG | 1 cut(s) | 1409 |
| BseMII | CTCAG | 5 cut(s) | 395, 464, 828, 989, 1542 |
| BseNI | ACTGG | 3 cut(s) | 460, 737, 910 |
| BseRI | GAGGAG | 7 cut(s) | 74, 607, 854, 1015, 1121, 1124, 1583 |
| BseXI | GCAGC | 6 cut(s) | 384, 568, 993, 1179, 1267, 1573 |
| Bsh1236I | CGCG | 3 cut(s) | 853, 977, 1230 |
| Bsh1285I | CGRYCG | 1 cut(s) | 219 |
| BshFI | GGCC | 3 cut(s) | 680, 894, 935 |
| BshVI | ATCGAT | 1 cut(s) | 1053 |
| BsiEI | CGRYCG | 1 cut(s) | 219 |
| BsiHKAI | GWGCWC | 3 cut(s) | 124, 410, 756 |
| BsiHKCI | CYCGRG | 1 cut(s) | 898 |
| BsiSI | CCGG | 9 cut(s) | 185, 354, 537, 578, 697, 899, 1188, 1397, 1476 |
| BsiWI | CGTACG | 1 cut(s) | 570 |
| BslFI | GGGAC | 2 cut(s) | 644, 916 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 178, 360, 670, 905, 999, 1403 |
| BsmAI | GTCTC | 2 cut(s) | 764, 1010 |
| BsmBI | CGTCTC | 1 cut(s) | 1010 |
| BsmFI | GGGAC | 2 cut(s) | 644, 916 |
| BsnI | GGCC | 3 cut(s) | 680, 894, 935 |
| BsoBI | CYCGRG | 1 cut(s) | 898 |
| Bsp119I | TTCGAA | 1 cut(s) | 274 |
| Bsp1286I | GDGCHC | 4 cut(s) | 124, 170, 410, 756 |
| Bsp13I | TCCGGA | 1 cut(s) | 1396 |
| Bsp1407I | TGTACA | 1 cut(s) | 94 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 552 |
| Bsp19I | CCATGG | 1 cut(s) | 930 |
| BspANI | GGCC | 3 cut(s) | 680, 894, 935 |
| BspCNI | CTCAG | 5 cut(s) | 396, 465, 829, 990, 1543 |
| BspDI | ATCGAT | 1 cut(s) | 1053 |
| BspEI | TCCGGA | 1 cut(s) | 1396 |
| BspFNI | CGCG | 3 cut(s) | 853, 977, 1230 |
| BspLI | GGNNCC | 7 cut(s) | 183, 211, 632, 646, 737, 895, 1128 |
| BspMAI | CTGCAG | 2 cut(s) | 1500, 1591 |
| BspPI | GGATC | 4 cut(s) | 204, 217, 1121, 1134 |
| BspT104I | TTCGAA | 1 cut(s) | 274 |
| BsrBI | CCGCTC | 1 cut(s) | 361 |
| BsrDI | GCAATG | 1 cut(s) | 1409 |
| BsrFI | RCCGGY | 1 cut(s) | 696 |
| BsrGI | TGTACA | 1 cut(s) | 94 |
| BsrI | ACTGG | 3 cut(s) | 460, 737, 910 |
| BssAI | RCCGGY | 1 cut(s) | 696 |
| BssECI | CCNNGG | 9 cut(s) | 205, 354, 897, 898, 899, 930, 975, 1228, 1476 |
| BssNI | GRCGYC | 2 cut(s) | 533, 1019 |
| BssSI | CACGAG | 1 cut(s) | 488 |
| BssT1I | CCWWGG | 1 cut(s) | 930 |
| Bst2BI | CACGAG | 1 cut(s) | 488 |
| Bst2UI | CCWGG | 1 cut(s) | 1175 |
| Bst4CI | ACNGT | 1 cut(s) | 1331 |
| Bst6I | CTCTTC | 1 cut(s) | 866 |
| BstACI | GRCGYC | 2 cut(s) | 533, 1019 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1459 |
| BstAUI | TGTACA | 1 cut(s) | 94 |
| BstBAI | YACGTR | 1 cut(s) | 1264 |
| BstBI | TTCGAA | 1 cut(s) | 274 |
| BstC8I | GCNNGC | 5 cut(s) | 426, 864, 1225, 1356, 1496 |
| BstDEI | CTNAG | 8 cut(s) | 404, 473, 552, 653, 837, 998, 1464, 1551 |
| BstDSI | CCRYGG | 3 cut(s) | 930, 975, 1228 |
| BstEII | GGTNACC | 1 cut(s) | 878 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 668 |
| BstF5I | GGATG | 4 cut(s) | 4, 505, 1308, 1486 |
| BstFNI | CGCG | 3 cut(s) | 853, 977, 1230 |
| BstH2I | RGCGCY | 1 cut(s) | 436 |
| BstHHI | GCGC | 3 cut(s) | 372, 435, 853 |
| BstMAI | GTCTC | 2 cut(s) | 764, 1010 |
| BstMCI | CGRYCG | 1 cut(s) | 219 |
| BstNI | CCWGG | 1 cut(s) | 1175 |
| BstNSI | RCATGY | 1 cut(s) | 284 |
| BstPI | GGTNACC | 1 cut(s) | 878 |
| BstSCI | CCNGG | 7 cut(s) | 353, 536, 897, 898, 1173, 1187, 1475 |
| BstSFI | CTRYAG | 2 cut(s) | 1496, 1587 |
| BstUI | CGCG | 3 cut(s) | 853, 977, 1230 |
| BstV1I | GCAGC | 6 cut(s) | 384, 568, 993, 1179, 1267, 1573 |
| BstX2I | RGATCY | 2 cut(s) | 209, 1126 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 641 |
| BstYI | RGATCY | 2 cut(s) | 209, 1126 |
| Bsu15I | ATCGAT | 1 cut(s) | 1053 |
| BsuI | GTATCC | 2 cut(s) | 330, 1603 |
| BsuRI | GGCC | 3 cut(s) | 680, 894, 935 |
| BsuTUI | ATCGAT | 1 cut(s) | 1053 |
| BtgI | CCRYGG | 3 cut(s) | 930, 975, 1228 |
| BtsCI | GGATG | 4 cut(s) | 4, 505, 1308, 1486 |
| BtsIMutI | CAGTG | 1 cut(s) | 730 |
| Cac8I | GCNNGC | 5 cut(s) | 426, 864, 1225, 1356, 1496 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1504 |
| CfoI | GCGC | 3 cut(s) | 372, 435, 853 |
| Cfr10I | RCCGGY | 1 cut(s) | 696 |
| Cfr13I | GGNCC | 3 cut(s) | 631, 644, 893 |
| Cfr42I | CCGCGG | 2 cut(s) | 978, 1231 |
| Cfr9I | CCCGGG | 1 cut(s) | 898 |
| ClaI | ATCGAT | 1 cut(s) | 1053 |
| CseI | GACGC | 1 cut(s) | 522 |
| Csp6I | GTAC | 6 cut(s) | 95, 256, 292, 544, 571, 598 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1245, 1280 |
| CviAII | CATG | 7 cut(s) | 178, 281, 478, 659, 740, 919, 931 |
| CviQI | GTAC | 6 cut(s) | 95, 256, 292, 544, 571, 598 |
| DdeI | CTNAG | 8 cut(s) | 404, 473, 552, 653, 837, 998, 1464, 1551 |
| DraI | TTTAAA | 1 cut(s) | 66 |
| EaeI | YGGCCR | 1 cut(s) | 933 |
| Eam1104I | CTCTTC | 1 cut(s) | 866 |
| EarI | CTCTTC | 1 cut(s) | 866 |
| EciI | GGCGGA | 2 cut(s) | 376, 803 |
| Ecl136II | GAGCTC | 1 cut(s) | 408 |
| Eco130I | CCWWGG | 1 cut(s) | 930 |
| Eco24I | GRGCYC | 2 cut(s) | 170, 410 |
| Eco47I | GGWCC | 2 cut(s) | 631, 644 |
| Eco53kI | GAGCTC | 1 cut(s) | 408 |
| Eco57I | CTGAAG | 1 cut(s) | 686 |
| Eco88I | CYCGRG | 1 cut(s) | 898 |
| Eco91I | GGTNACC | 1 cut(s) | 878 |
| EcoICRI | GAGCTC | 1 cut(s) | 408 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 668 |
| EcoO65I | GGTNACC | 1 cut(s) | 878 |
| EcoRII | CCWGG | 1 cut(s) | 1173 |
| EcoT14I | CCWWGG | 1 cut(s) | 930 |
| EcoT22I | ATGCAT | 1 cut(s) | 6 |
| EcoT38I | GRGCYC | 2 cut(s) | 170, 410 |
| ErhI | CCWWGG | 1 cut(s) | 930 |
| Esp3I | CGTCTC | 1 cut(s) | 1010 |
| FaeI | CATG | 7 cut(s) | 181, 284, 481, 662, 743, 922, 934 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 431, 463 |
| FaqI | GGGAC | 2 cut(s) | 644, 916 |
| FatI | CATG | 7 cut(s) | 177, 280, 477, 658, 739, 918, 930 |
| FauI | CCCGC | 2 cut(s) | 215, 855 |
| Fnu4HI | GCNGC | 9 cut(s) | 362, 373, 557, 927, 1007, 1193, 1228, 1281, 1587 |
| FokI | GGATG | 3 cut(s) | 512, 1315, 1493 |
| FriOI | GRGCYC | 2 cut(s) | 170, 410 |
| Fsp4HI | GCNGC | 9 cut(s) | 362, 373, 557, 927, 1007, 1193, 1228, 1281, 1587 |
| FspBI | CTAG | 2 cut(s) | 113, 1359 |
| FspI | TGCGCA | 1 cut(s) | 371 |
| GlaI | GCGC | 3 cut(s) | 371, 434, 852 |
| GluI | GCNGC | 9 cut(s) | 362, 373, 557, 927, 1007, 1193, 1228, 1281, 1587 |
| GsuI | CTGGAG | 3 cut(s) | 754, 1419, 1502 |
| HaeII | RGCGCY | 1 cut(s) | 436 |
| HaeIII | GGCC | 3 cut(s) | 680, 894, 935 |
| HapII | CCGG | 9 cut(s) | 185, 354, 537, 578, 697, 899, 1188, 1397, 1476 |
| HgaI | GACGC | 1 cut(s) | 522 |
| HhaI | GCGC | 3 cut(s) | 372, 435, 853 |
| Hin1I | GRCGYC | 2 cut(s) | 533, 1019 |
| Hin1II | CATG | 7 cut(s) | 181, 284, 481, 662, 743, 922, 934 |
| Hin6I | GCGC | 3 cut(s) | 370, 433, 851 |
| HinP1I | GCGC | 3 cut(s) | 370, 433, 851 |
| HindIII | AAGCTT | 2 cut(s) | 707, 1271 |
| HinfI | GANTC | 7 cut(s) | 516, 772, 1340, 1379, 1432, 1501, 1616 |
| HpaII | CCGG | 9 cut(s) | 185, 354, 537, 578, 697, 899, 1188, 1397, 1476 |
| HphI | GGTGA | 3 cut(s) | 343, 592, 1611 |
| Hpy166II | GTNNAC | 3 cut(s) | 351, 583, 1251 |
| Hpy188I | TCNGA | 6 cut(s) | 105, 209, 333, 474, 819, 1131 |
| Hpy188III | TCNNGA | 6 cut(s) | 451, 488, 664, 1397, 1519, 1607 |
| Hpy8I | GTNNAC | 3 cut(s) | 351, 583, 1251 |
| HpyCH4III | ACNGT | 1 cut(s) | 1331 |
| HpyCH4IV | ACGT | 2 cut(s) | 1019, 1263 |
| HpyCH4V | TGCA | 7 cut(s) | 4, 157, 761, 1283, 1448, 1498, 1589 |
| HpyF3I | CTNAG | 8 cut(s) | 404, 473, 552, 653, 837, 998, 1464, 1551 |
| HpySE526I | ACGT | 2 cut(s) | 1019, 1263 |
| Hsp92I | GRCGYC | 2 cut(s) | 533, 1019 |
| Hsp92II | CATG | 7 cut(s) | 181, 284, 481, 662, 743, 922, 934 |
| HspAI | GCGC | 3 cut(s) | 370, 433, 851 |
| Kpn2I | TCCGGA | 1 cut(s) | 1396 |
| KspI | CCGCGG | 2 cut(s) | 978, 1231 |
| LmnI | GCTCC | 5 cut(s) | 358, 413, 580, 735, 1406 |
| Lsp1109I | GCAGC | 6 cut(s) | 384, 568, 993, 1179, 1267, 1573 |
| LweI | GCATC | 4 cut(s) | 13, 357, 1267, 1449 |
| MaeI | CTAG | 2 cut(s) | 113, 1359 |
| MaeII | ACGT | 2 cut(s) | 1019, 1263 |
| MaeIII | GTNAC | 4 cut(s) | 726, 878, 1177, 1331 |
| MbiI | CCGCTC | 1 cut(s) | 361 |
| MboII | GAAGA | 6 cut(s) | 141, 883, 1150, 1568, 1604, 1607 |
| MflI | RGATCY | 2 cut(s) | 209, 1126 |
| MhlI | GDGCHC | 4 cut(s) | 124, 170, 410, 756 |
| MlsI | TGGCCA | 1 cut(s) | 935 |
| MluCI | AATT | 7 cut(s) | 51, 67, 90, 99, 1198, 1319, 1401 |
| MluNI | TGGCCA | 1 cut(s) | 935 |
| MlyI | GAGTC | 4 cut(s) | 525, 766, 1426, 1510 |
| MmeI | TCCRAC | 1 cut(s) | 128 |
| Mox20I | TGGCCA | 1 cut(s) | 935 |
| Mph1103I | ATGCAT | 1 cut(s) | 6 |
| MroI | TCCGGA | 1 cut(s) | 1396 |
| MscI | TGGCCA | 1 cut(s) | 935 |
| MseI | TTAA | 7 cut(s) | 54, 65, 72, 77, 227, 981, 1425 |
| Msp20I | TGGCCA | 1 cut(s) | 935 |
| MspA1I | CMGCKG | 3 cut(s) | 977, 1230, 1439 |
| MspI | CCGG | 9 cut(s) | 185, 354, 537, 578, 697, 899, 1188, 1397, 1476 |
| MspR9I | CCNGG | 7 cut(s) | 355, 538, 899, 900, 1175, 1189, 1477 |
| MvaI | CCWGG | 1 cut(s) | 1175 |
| MvnI | CGCG | 3 cut(s) | 853, 977, 1230 |
| NciI | CCSGG | 6 cut(s) | 355, 538, 899, 900, 1189, 1477 |
| NcoI | CCATGG | 1 cut(s) | 930 |
| NlaIII | CATG | 7 cut(s) | 181, 284, 481, 662, 743, 922, 934 |
| NlaIV | GGNNCC | 7 cut(s) | 183, 211, 632, 646, 737, 895, 1128 |
| NmuCI | GTSAC | 1 cut(s) | 726 |
| NsbI | TGCGCA | 1 cut(s) | 371 |
| NsiI | ATGCAT | 1 cut(s) | 6 |
| NspI | RCATGY | 1 cut(s) | 284 |
| NspV | TTCGAA | 1 cut(s) | 274 |
| PfeI | GAWTC | 3 cut(s) | 1340, 1379, 1616 |
| Pfl23II | CGTACG | 1 cut(s) | 570 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 178 |
| PfoI | TCCNGGA | 1 cut(s) | 536 |
| PkrI | GCNGC | 9 cut(s) | 363, 374, 558, 928, 1008, 1194, 1229, 1282, 1588 |
| Ple19I | CGATCG | 1 cut(s) | 219 |
| PleI | GAGTC | 4 cut(s) | 524, 766, 1426, 1509 |
| PpsI | GAGTC | 4 cut(s) | 524, 766, 1426, 1509 |
| Ppu21I | YACGTR | 1 cut(s) | 1264 |
| Psp124BI | GAGCTC | 1 cut(s) | 410 |
| Psp6I | CCWGG | 1 cut(s) | 1173 |
| PspEI | GGTNACC | 1 cut(s) | 878 |
| PspGI | CCWGG | 1 cut(s) | 1173 |
| PspLI | CGTACG | 1 cut(s) | 570 |
| PspN4I | GGNNCC | 7 cut(s) | 183, 211, 632, 646, 737, 895, 1128 |
| PspPI | GGNCC | 3 cut(s) | 631, 644, 893 |
| PstI | CTGCAG | 2 cut(s) | 1500, 1591 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1504 |
| PsuI | RGATCY | 2 cut(s) | 209, 1126 |
| PvuI | CGATCG | 1 cut(s) | 219 |
| PvuII | CAGCTG | 1 cut(s) | 1439 |
| RsaI | GTAC | 6 cut(s) | 96, 257, 293, 545, 572, 599 |
| RsaNI | GTAC | 6 cut(s) | 95, 256, 292, 544, 571, 598 |
| SacI | GAGCTC | 1 cut(s) | 410 |
| SacII | CCGCGG | 2 cut(s) | 978, 1231 |
| SaqAI | TTAA | 7 cut(s) | 54, 65, 72, 77, 227, 981, 1425 |
| SatI | GCNGC | 9 cut(s) | 362, 373, 557, 927, 1007, 1193, 1228, 1281, 1587 |
| Sau96I | GGNCC | 3 cut(s) | 631, 644, 893 |
| ScaI | AGTACT | 2 cut(s) | 257, 293 |
| SchI | GAGTC | 4 cut(s) | 525, 766, 1426, 1510 |
| ScrFI | CCNGG | 7 cut(s) | 355, 538, 899, 900, 1175, 1189, 1477 |
| SduI | GDGCHC | 4 cut(s) | 124, 170, 410, 756 |
| SfaNI | GCATC | 4 cut(s) | 13, 357, 1267, 1449 |
| SfcI | CTRYAG | 2 cut(s) | 1496, 1587 |
| Sfr303I | CCGCGG | 2 cut(s) | 978, 1231 |
| SfuI | TTCGAA | 1 cut(s) | 274 |
| SgrBI | CCGCGG | 2 cut(s) | 978, 1231 |
| SinI | GGWCC | 2 cut(s) | 631, 644 |
| SmaI | CCCGGG | 1 cut(s) | 900 |
| SmlI | CTYRAG | 3 cut(s) | 437, 965, 1370 |
| SmoI | CTYRAG | 3 cut(s) | 437, 965, 1370 |
| Sse9I | AATT | 7 cut(s) | 51, 67, 90, 99, 1198, 1319, 1401 |
| SspMI | CTAG | 2 cut(s) | 113, 1359 |
| SstI | GAGCTC | 1 cut(s) | 410 |
| StyD4I | CCNGG | 7 cut(s) | 353, 536, 897, 898, 1173, 1187, 1475 |
| StyI | CCWWGG | 1 cut(s) | 930 |
| TaaI | ACNGT | 1 cut(s) | 1331 |
| TaiI | ACGT | 2 cut(s) | 1022, 1266 |
| TaqI | TCGA | 8 cut(s) | 274, 285, 393, 810, 1031, 1053, 1377, 1485 |
| TasI | AATT | 7 cut(s) | 51, 67, 90, 99, 1198, 1319, 1401 |
| TatI | WGTACW | 4 cut(s) | 94, 255, 291, 543 |
| TauI | GCSGC | 3 cut(s) | 364, 929, 1230 |
| TfiI | GAWTC | 3 cut(s) | 1340, 1379, 1616 |
| Tru1I | TTAA | 7 cut(s) | 54, 65, 72, 77, 227, 981, 1425 |
| Tru9I | TTAA | 7 cut(s) | 54, 65, 72, 77, 227, 981, 1425 |
| TscAI | CASTG | 1 cut(s) | 737 |
| TseFI | GTSAC | 1 cut(s) | 726 |
| TseI | GCWGC | 6 cut(s) | 372, 556, 1006, 1192, 1280, 1586 |
| Tsp45I | GTSAC | 1 cut(s) | 726 |
| TspDTI | ATGAA | 2 cut(s) | 809, 1192 |
| TspMI | CCCGGG | 1 cut(s) | 898 |
| TspRI | CASTG | 1 cut(s) | 737 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 178 |
| VpaK11BI | GGWCC | 2 cut(s) | 631, 644 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 668 |
| XapI | RAATTY | 3 cut(s) | 67, 99, 1198 |
| XceI | RCATGY | 1 cut(s) | 284 |
| XmaI | CCCGGG | 1 cut(s) | 898 |
| XspI | CTAG | 2 cut(s) | 113, 1359 |
| ZraI | GACGTC | 1 cut(s) | 1020 |
| ZrmI | AGTACT | 2 cut(s) | 257, 293 |
| Zsp2I | ATGCAT | 1 cut(s) | 6 |