FvH4_2g07230

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000235
Physical Location & Seq
Forward (+)
5990431 .. 5993351
2921 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g07230.t1

Sequence Viewer

Length: 831 bp
ATGTCTATGCAATTCGATGATTTTGGAGGGGCGAGAAGATTAGTGATGGGTGTGGAGGGGAATAGATGGCCAAATGGAAAGCCCCAAAATGTAACAGCTCCTAGCCTCTTGAGTAGATTTGTGCCTAGCATATATTTGTTTTGGTCAAGAGTAAAAGCAAGGCACAGACTAGGACTGAGGAATGTATTGCTTGCCTCATCTTGCCCAGTGCCCACACACATTGTTTCAGCAATACAACACAACAGCAACCATCCAGAATTGCACAGTGACCAGCAATTGTTGAGGCCTCACAGAAGCCCCCATTTTGGCTCTTCATCCAGATACAACTTCATTGCCAGAAATATCTCACTCACTGACACAATCCATCAGCAAAAGGAAATTGCAGTGCTCCACTCTCCACTGCAACACATCTTGAATGAACTCTCTATAAGGGCAACTTTGACTCTAAGCAGTTCTGCAGATAAATTATGGAATGTCAGAGTCCATAAAACAGAAAGGAATATTTATCTCAAGGAAGGCTGGCAAGAGTTTCTAAGAGATAACGCCATAGGTGATTCAGAGTTCTTGGTGTTTGGATATGATGGAAACATGCATTTCAGCATAGATATTTATGAGAGGACTGGGTGTAAGAGAACTGTAGCTCCCGCTATTCAAGGGCATCAACGTTCCACCTTCACCAACAATACTAAAAGACTTTGTGGTAGACCAAGAAAGAGCGGCACTGCAGCTGAACACACTGCAAATGGTGAATCCAGTAACAATACTAAAAGACATCGTGGTAGACAAAGAGTGAGCGATAATGCAGCTGAACACGTACACTGCAAATGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0007275 GO:0008150 GO:0009553 GO:0009566 GO:0009567 GO:0019953 GO:0022414 GO:0032501 GO:0032502 GO:0044703 GO:0048229 GO:0048856 GO:0051704
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

277

Amino Acids

31.41

Weight (kDa)

10.2

Isoelectric Point (pI)

58.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 143 - 205 4.6e-11 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000235)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g22700 FvH4_2g06920 FvH4_2g06920 FvH4_2g06960 FvH4_2g06960 FvH4_2g06960 FvH4_2g06960 FvH4_2g06970 FvH4_2g06970 FvH4_2g07230 FvH4_2g07231 FvH4_2g07231 FvH4_2g07240 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_4g19990 FvH4_4g19990 FvH4_4g19990 FvH4_4g20070
malus_domestica MD05G1072700.v1.1
prunus_persica Prupe.8G113100_v2.0.a1
pyrus_communis pycom05g06500 pycom10g06950
rosa_chinensis RchiOBHm_Chr4g0425711 RchiOBHm_Chr6g0259241 RchiOBHm_Chr6g0259251 RchiOBHm_Chr6g0259321 RchiOBHm_Chr6g0259331 RchiOBHm_Chr6g0259781 RchiOBHm_Chr6g0259791 RchiOBHm_Chr6g0259811 RchiOBHm_Chr6g0259881 RchiOBHm_Chr6g0259931
rosa_laevigata RLG00000007345 RLG00000014500 RLG00000014501 RLG00000014505 RLG00000014506 RLG00000014507 RLG00000014539 RLG00000014540 RLG00000014545 RLG00000014546
rosa_multiflora Rmu_co8137334.1_g000001 Rmu_co8454279.1_g000001 Rmu_co8476373.1_g000001 Rmu_sc0001078.1_g000001 Rmu_sc0003958.1_g000011 Rmu_sc0009049.1_g000001 Rmu_sc0009049.1_g000009 Rmu_sc0010546.1_g000001 Rmu_sc0011974.1_g000002 Rmu_sc0011974.1_g000003 Rmu_sc0033846.1_g000001 Rmu_sc0036999.1_g000001 Rmu_ssc0000052.1_g000015
rosa_roxburghii Rroxscaffold_5G00367690 Rroxscaffold_7G00206530 Rroxscaffold_7G00206540 Rroxscaffold_7G00206560 Rroxscaffold_7G00206570 Rroxscaffold_7G00207020 Rroxscaffold_7G00207030 Rroxscaffold_7G00207150 Rroxscaffold_7G00207160 Rroxscaffold_7G00207170 Rroxscaffold_7G00207270
rosa_rugosa Rorug04G0205300 Rorug05G0586300 Rorug05G0586400 Rorug05G0586500 Rorug05G0586600 Rorug05G0586700 Rorug05G0587300 Rorug05G0587400 Rorug05G0587500 Rorug05G0587600 Rorug05G0590500 Rorug05G0590600 Rorug05G0591200 Rorug05G0591300 Rorug05G0591400 Rorug05G0591500
rosa_samantha Rh4AG262200 Rh4BG268500 Rh4CG279700 Rh4DG264100 Rh6AG103100 Rh6AG103600 Rh6AG107100 Rh6AG107200 Rh6AG107700 Rh6BG095400 Rh6BG095500 Rh6BG095900 Rh6BG096000 Rh6BG096800 Rh6BG102600 Rh6BG102700 Rh6BG102800 Rh6BG103800 Rh6CG092300 Rh6CG092400 Rh6CG092800 Rh6CG092900 Rh6CG096200 Rh6CG096300 Rh6CG096800 Rh6CG097100 Rh6DG086300 Rh6DG086400 Rh6DG086700 Rh6DG086800 Rh6DG090000 Rh6DG090100 Rh6DG090500 Rh6DG090800
rosa_wichuraiana Rw4G022670 Rw6G008900 Rw6G008940 Rw6G009250 Rw6G009280 Rw6G009330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 717
AccI GTMKAC 2 cut(s) 703, 781
AciI CCGC 2 cut(s) 645, 717
AclI AACGTT 1 cut(s) 664
AcoI YGGCCR 1 cut(s) 68
AfaI GTAC 1 cut(s) 816
AfiI CCNNNNNNNGG 1 cut(s) 305
AflIII ACRYGT 1 cut(s) 811
AgsI TTSAA 2 cut(s) 415, 653
AloI GAACNNNNNNTCC 2 cut(s) 625, 657
AluBI AGCT 4 cut(s) 98, 641, 728, 806
AluI AGCT 4 cut(s) 98, 641, 728, 806
Alw21I GWGCWC 1 cut(s) 390
AoxI GGCC 2 cut(s) 68, 284
ApeKI GCWGC 2 cut(s) 725, 803
AsuHPI GGTGA 3 cut(s) 563, 667, 758
BaeGI GKGCMC 1 cut(s) 213
BalI TGGCCA 1 cut(s) 70
Bbv12I GWGCWC 1 cut(s) 390
BbvI GCAGC 2 cut(s) 737, 815
BccI CCATC 5 cut(s) 40, 60, 258, 372, 575
BfaI CTAG 3 cut(s) 102, 126, 170
BfmI CTRYAG 3 cut(s) 456, 636, 723
BisI GCNGC 3 cut(s) 718, 726, 804
BlsI GCNGC 3 cut(s) 719, 727, 805
BmrI ACTGGG 2 cut(s) 200, 630
BmsI GCATC 1 cut(s) 667
BmuI ACTGGG 2 cut(s) 200, 630
BpuEI CTTGAG 2 cut(s) 130, 494
BsaAI YACGTR 1 cut(s) 814
BsaXI ACNNNNNCTCC 2 cut(s) 625, 655
Bsc4I CCNNNNNNNGG 1 cut(s) 305
Bse1I ACTGG 3 cut(s) 206, 625, 753
Bse3DI GCAATG 1 cut(s) 330
BseGI GGATG 2 cut(s) 250, 314
BseLI CCNNNNNNNGG 1 cut(s) 305
BseMI GCAATG 1 cut(s) 330
BseMII CTCAG 1 cut(s) 167
BseNI ACTGG 3 cut(s) 206, 625, 753
BseSI GKGCMC 1 cut(s) 213
BseXI GCAGC 2 cut(s) 737, 815
BshFI GGCC 2 cut(s) 70, 286
BsiHKAI GWGCWC 1 cut(s) 390
BslI CCNNNNNNNGG 1 cut(s) 305
BsnI GGCC 2 cut(s) 70, 286
Bsp1286I GDGCHC 2 cut(s) 213, 390
BspACI CCGC 2 cut(s) 645, 717
BspANI GGCC 2 cut(s) 70, 286
BspCNI CTCAG 1 cut(s) 168
BspMAI CTGCAG 2 cut(s) 460, 727
BspQI GCTCTTC 1 cut(s) 316
BsrBI CCGCTC 1 cut(s) 717
BsrDI GCAATG 1 cut(s) 330
BsrI ACTGG 3 cut(s) 206, 625, 753
Bst4CI ACNGT 2 cut(s) 266, 637
Bst6I CTCTTC 1 cut(s) 316
BstBAI YACGTR 1 cut(s) 814
BstC8I GCNNGC 2 cut(s) 192, 521
BstDEI CTNAG 3 cut(s) 176, 446, 533
BstF5I GGATG 2 cut(s) 250, 314
BstNSI RCATGY 1 cut(s) 592
BstSFI CTRYAG 3 cut(s) 456, 636, 723
BstSLI GKGCMC 1 cut(s) 213
BstV1I GCAGC 2 cut(s) 737, 815
BsuRI GGCC 2 cut(s) 70, 286
BtsCI GGATG 2 cut(s) 250, 314
BtsI GCAGTG 5 cut(s) 390, 398, 720, 735, 817
BtsIMutI CAGTG 8 cut(s) 213, 271, 351, 390, 398, 720, 735, 817
Cac8I GCNNGC 2 cut(s) 192, 521
Csp6I GTAC 1 cut(s) 815
CspCI CAANNNNNGTGG 2 cut(s) 202, 237
CviAII CATG 1 cut(s) 589
CviQI GTAC 1 cut(s) 815
DdeI CTNAG 3 cut(s) 176, 446, 533
EaeI YGGCCR 1 cut(s) 68
Eam1104I CTCTTC 1 cut(s) 316
EarI CTCTTC 1 cut(s) 316
Eco147I AGGCCT 1 cut(s) 286
EcoT22I ATGCAT 1 cut(s) 594
FaeI CATG 1 cut(s) 592
FalI AAGNNNNNCTT 2 cut(s) 421, 453
FatI CATG 1 cut(s) 588
FauI CCCGC 1 cut(s) 652
FblI GTMKAC 2 cut(s) 703, 781
Fnu4HI GCNGC 3 cut(s) 718, 726, 804
FokI GGATG 2 cut(s) 237, 301
Fsp4HI GCNGC 3 cut(s) 718, 726, 804
FspBI CTAG 3 cut(s) 102, 126, 170
GluI GCNGC 3 cut(s) 718, 726, 804
HaeIII GGCC 2 cut(s) 70, 286
Hin1II CATG 1 cut(s) 592
HinfI GANTC 4 cut(s) 442, 480, 554, 749
HphI GGTGA 3 cut(s) 563, 667, 758
Hpy166II GTNNAC 3 cut(s) 704, 782, 817
Hpy188I TCNGA 2 cut(s) 479, 559
Hpy188III TCNNGA 5 cut(s) 109, 147, 254, 318, 412
Hpy8I GTNNAC 3 cut(s) 704, 782, 817
HpyAV CCTTC 2 cut(s) 509, 682
HpyCH4III ACNGT 2 cut(s) 266, 637
HpyCH4IV ACGT 2 cut(s) 664, 813
HpyF3I CTNAG 3 cut(s) 176, 446, 533
HpySE526I ACGT 2 cut(s) 664, 813
Hsp92II CATG 1 cut(s) 592
LguI GCTCTTC 1 cut(s) 316
LmnI GCTCC 3 cut(s) 103, 393, 646
LpnPI CCDG 8 cut(s) 219, 267, 284, 331, 349, 505, 606, 766
Lsp1109I GCAGC 2 cut(s) 737, 815
LweI GCATC 1 cut(s) 667
MaeI CTAG 3 cut(s) 102, 126, 170
MaeII ACGT 2 cut(s) 664, 813
MaeIII GTNAC 3 cut(s) 91, 266, 755
MbiI CCGCTC 1 cut(s) 717
MboII GAAGA 2 cut(s) 48, 303
MfeI CAATTG 1 cut(s) 275
MhlI GDGCHC 2 cut(s) 213, 390
MlsI TGGCCA 1 cut(s) 70
MluCI AATT 5 cut(s) 11, 257, 275, 378, 464
MluNI TGGCCA 1 cut(s) 70
MlyI GAGTC 2 cut(s) 436, 489
MnlI CCTC 8 cut(s) 20, 49, 116, 171, 205, 276, 297, 609
Mox20I TGGCCA 1 cut(s) 70
Mph1103I ATGCAT 1 cut(s) 594
MscI TGGCCA 1 cut(s) 70
Msp20I TGGCCA 1 cut(s) 70
MspA1I CMGCKG 2 cut(s) 728, 806
MunI CAATTG 1 cut(s) 275
NlaIII CATG 1 cut(s) 592
NmuCI GTSAC 1 cut(s) 266
NsiI ATGCAT 1 cut(s) 594
NspI RCATGY 1 cut(s) 592
PceI AGGCCT 1 cut(s) 286
PciSI GCTCTTC 1 cut(s) 316
PfeI GAWTC 2 cut(s) 554, 749
PkrI GCNGC 3 cut(s) 719, 727, 805
PleI GAGTC 2 cut(s) 436, 488
PpsI GAGTC 2 cut(s) 436, 488
Ppu21I YACGTR 1 cut(s) 814
Psp1406I AACGTT 1 cut(s) 664
PstI CTGCAG 2 cut(s) 460, 727
PvuII CAGCTG 2 cut(s) 728, 806
RsaI GTAC 1 cut(s) 816
RsaNI GTAC 1 cut(s) 815
SapI GCTCTTC 1 cut(s) 316
SatI GCNGC 3 cut(s) 718, 726, 804
SchI GAGTC 2 cut(s) 436, 489
SduI GDGCHC 2 cut(s) 213, 390
SetI ASST 8 cut(s) 100, 553, 643, 667, 674, 730, 808, 816
SfaNI GCATC 1 cut(s) 667
SfcI CTRYAG 3 cut(s) 456, 636, 723
SmlI CTYRAG 2 cut(s) 109, 509
SmoI CTYRAG 2 cut(s) 109, 509
Sse9I AATT 5 cut(s) 11, 257, 275, 378, 464
SseBI AGGCCT 1 cut(s) 286
SsiI CCGC 2 cut(s) 645, 717
SspI AATATT 1 cut(s) 502
SspMI CTAG 3 cut(s) 102, 126, 170
StuI AGGCCT 1 cut(s) 286
TaaI ACNGT 2 cut(s) 266, 637
TaiI ACGT 2 cut(s) 667, 816
TaqI TCGA 1 cut(s) 15
TasI AATT 5 cut(s) 11, 257, 275, 378, 464
TauI GCSGC 1 cut(s) 720
TfiI GAWTC 2 cut(s) 554, 749
TscAI CASTG 8 cut(s) 213, 271, 358, 390, 405, 727, 742, 824
TseFI GTSAC 1 cut(s) 266
TseI GCWGC 2 cut(s) 725, 803
Tsp45I GTSAC 1 cut(s) 266
TspDTI ATGAA 3 cut(s) 303, 319, 432
TspRI CASTG 8 cut(s) 213, 271, 358, 390, 405, 727, 742, 824
XceI RCATGY 1 cut(s) 592
XmiI GTMKAC 2 cut(s) 703, 781
XspI CTAG 3 cut(s) 102, 126, 170
Zsp2I ATGCAT 1 cut(s) 594
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.