RLG00000014501

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0000235
Physical Location & Seq
Reverse (-)
54025840 .. 54027388
1549 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014501

Sequence Viewer

Length: 897 bp
ATGGTGAGACCCAAGAATGGTATCAAAGAAAAGCAGCCTTCATTTTTCAAGGTTATTATTCCAGGCTACTCCACTCAACTTTTGAGGATCCCACCACCATTCTTGGAGTTTATAAGGAATGACCTATCTGATGAAGCAACCTTGAACTCGAACACTTGTTCAGAGAGGTCTTGGAATGTTAGAGTACGTAAAACAAAAGGAGGTGTGTATTTCAAGGATGGGTGGCAGGGGTTCTTAAAAGATAACTCTGTGGAAGATGGTGACTTTTTGGTAGTCGAGTATGAAGGAAACATGCATTTCAGCGTACAGTTTTTTGACAACAGTTGTTGTGAGAGAGACTCTTTTCCTGACATTGAAACACACACAAAATCCACCTTTGCTGAGACTAAGAAAAGGCATCCTGGTAGATCGAGGAAATCAGATATCGGTTGTTCTAAGCGTCCCCTCGCATTGAACTCTAGAAAAGACAATTCAGATAACATAGCTAAGAAAAGACTTCCTGGTAGACCAAGAAAATCCCATATCCGTCGTTATAAGCGCTCAGACTCTGGCGAAGACAATTCAGTCCCAGATGACATAGTTGAGGAATCTGCAAAATCTTTCAAGTCCAACTATCCTCATGTCACAAAAGCCATTAAAGAGACTTTTTGTGTGTACTCATTCCCCACATCCTTCTATAGGGAGCATCTCTCTGGGGGCAACTATGAAGTTGTTTTCCTGAAAATTGCAGGGGGGGAAAGATGGTACCAAGTGAAGCTTGTACGATCCCTAAGAACGGCATACTTATCCAAAGGGTGGTCTGAGTTTGCATGTGCTAACCAAATCAAGGTCGGAAATATCTGCGTATTTGAAGTTGTGGAAGAAAACAAATTGGTGGTTCACGTAGTCCAGCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0007275 GO:0008150 GO:0009553 GO:0009566 GO:0009567 GO:0019953 GO:0022414 GO:0032501 GO:0032502 GO:0044703 GO:0048229 GO:0048856 GO:0051704
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

299

Amino Acids

34.26

Weight (kDa)

9.31

Isoelectric Point (pI)

45.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 15 - 107 4.1e-16 B3 DNA binding domain
B3 PF02362 220 - 296 6.5e-12 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000235)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g22700 FvH4_2g06920 FvH4_2g06920 FvH4_2g06960 FvH4_2g06960 FvH4_2g06960 FvH4_2g06960 FvH4_2g06970 FvH4_2g06970 FvH4_2g07230 FvH4_2g07231 FvH4_2g07231 FvH4_2g07240 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_4g19990 FvH4_4g19990 FvH4_4g19990 FvH4_4g20070
malus_domestica MD05G1072700.v1.1
prunus_persica Prupe.8G113100_v2.0.a1
pyrus_communis pycom05g06500 pycom10g06950
rosa_chinensis RchiOBHm_Chr4g0425711 RchiOBHm_Chr6g0259241 RchiOBHm_Chr6g0259251 RchiOBHm_Chr6g0259321 RchiOBHm_Chr6g0259331 RchiOBHm_Chr6g0259781 RchiOBHm_Chr6g0259791 RchiOBHm_Chr6g0259811 RchiOBHm_Chr6g0259881 RchiOBHm_Chr6g0259931
rosa_laevigata RLG00000007345 RLG00000014500 RLG00000014501 RLG00000014505 RLG00000014506 RLG00000014507 RLG00000014539 RLG00000014540 RLG00000014545 RLG00000014546
rosa_multiflora Rmu_co8137334.1_g000001 Rmu_co8454279.1_g000001 Rmu_co8476373.1_g000001 Rmu_sc0001078.1_g000001 Rmu_sc0003958.1_g000011 Rmu_sc0009049.1_g000001 Rmu_sc0009049.1_g000009 Rmu_sc0010546.1_g000001 Rmu_sc0011974.1_g000002 Rmu_sc0011974.1_g000003 Rmu_sc0033846.1_g000001 Rmu_sc0036999.1_g000001 Rmu_ssc0000052.1_g000015
rosa_roxburghii Rroxscaffold_5G00367690 Rroxscaffold_7G00206530 Rroxscaffold_7G00206540 Rroxscaffold_7G00206560 Rroxscaffold_7G00206570 Rroxscaffold_7G00207020 Rroxscaffold_7G00207030 Rroxscaffold_7G00207150 Rroxscaffold_7G00207160 Rroxscaffold_7G00207170 Rroxscaffold_7G00207270
rosa_rugosa Rorug04G0205300 Rorug05G0586300 Rorug05G0586400 Rorug05G0586500 Rorug05G0586600 Rorug05G0586700 Rorug05G0587300 Rorug05G0587400 Rorug05G0587500 Rorug05G0587600 Rorug05G0590500 Rorug05G0590600 Rorug05G0591200 Rorug05G0591300 Rorug05G0591400 Rorug05G0591500
rosa_samantha Rh4AG262200 Rh4BG268500 Rh4CG279700 Rh4DG264100 Rh6AG103100 Rh6AG103600 Rh6AG107100 Rh6AG107200 Rh6AG107700 Rh6BG095400 Rh6BG095500 Rh6BG095900 Rh6BG096000 Rh6BG096800 Rh6BG102600 Rh6BG102700 Rh6BG102800 Rh6BG103800 Rh6CG092300 Rh6CG092400 Rh6CG092800 Rh6CG092900 Rh6CG096200 Rh6CG096300 Rh6CG096800 Rh6CG097100 Rh6DG086300 Rh6DG086400 Rh6DG086700 Rh6DG086800 Rh6DG090000 Rh6DG090100 Rh6DG090500 Rh6DG090800
rosa_wichuraiana Rw4G022670 Rw6G008900 Rw6G008940 Rw6G009250 Rw6G009280 Rw6G009330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 113, 534
AasI GACNNNNNNGTC 1 cut(s) 563
Acc65I GGTACC 1 cut(s) 744
AccB1I GGYRCC 1 cut(s) 744
AccB7I CCANNNNNTGG 1 cut(s) 795
AccI GTMKAC 1 cut(s) 505
AclWI GGATC 3 cut(s) 82, 95, 759
AfaI GTAC 5 cut(s) 186, 306, 656, 746, 762
AfeI AGCGCT 1 cut(s) 539
AfiI CCNNNNNNNGG 5 cut(s) 17, 678, 775, 795, 826
AgsI TTSAA 7 cut(s) 49, 145, 214, 356, 454, 604, 851
AjnI CCWGG 3 cut(s) 61, 400, 499
AluBI AGCT 2 cut(s) 485, 757
AluI AGCT 2 cut(s) 485, 757
Alw26I GTCTC 3 cut(s) 330, 377, 635
AlwI GGATC 3 cut(s) 82, 95, 759
AlwNI CAGNNNCTG 1 cut(s) 548
Aor51HI AGCGCT 1 cut(s) 539
ApeKI GCWGC 1 cut(s) 34
Asp718I GGTACC 1 cut(s) 744
AspLEI GCGC 1 cut(s) 540
AsuHPI GGTGA 2 cut(s) 16, 272
BamHI GGATCC 1 cut(s) 87
BanI GGYRCC 1 cut(s) 744
BbsI GAAGAC 1 cut(s) 561
BbvI GCAGC 1 cut(s) 46
BccI CCATC 3 cut(s) 212, 251, 735
BceAI ACGGC 1 cut(s) 792
BciT130I CCWGG 3 cut(s) 63, 402, 501
BcoDI GTCTC 3 cut(s) 330, 377, 635
BfaI CTAG 1 cut(s) 459
BfmI CTRYAG 1 cut(s) 676
BfoI RGCGCY 1 cut(s) 541
BisI GCNGC 1 cut(s) 35
BlsI GCNGC 1 cut(s) 36
Bme1390I CCNGG 3 cut(s) 63, 402, 501
BmiI GGNNCC 2 cut(s) 89, 746
BmrFI CCNGG 3 cut(s) 63, 402, 501
BmsI GCATC 2 cut(s) 406, 694
BpiI GAAGAC 1 cut(s) 561
BplI GAGNNNNNCTC 4 cut(s) 323, 355, 674, 706
BsaAI YACGTR 2 cut(s) 188, 883
Bsc4I CCNNNNNNNGG 5 cut(s) 17, 678, 775, 795, 826
BseBI CCWGG 3 cut(s) 63, 402, 501
BseGI GGATG 3 cut(s) 223, 397, 668
BseLI CCNNNNNNNGG 5 cut(s) 17, 678, 775, 795, 826
BseMII CTCAG 3 cut(s) 372, 555, 792
BseXI GCAGC 1 cut(s) 46
BshNI GGYRCC 1 cut(s) 744
BslFI GGGAC 2 cut(s) 426, 551
BslI CCNNNNNNNGG 5 cut(s) 17, 678, 775, 795, 826
BsmAI GTCTC 3 cut(s) 330, 377, 635
BsmFI GGGAC 2 cut(s) 426, 551
Bsp143I GATC 3 cut(s) 87, 407, 764
BspCNI CTCAG 3 cut(s) 373, 554, 793
BspLI GGNNCC 2 cut(s) 89, 746
BspPI GGATC 3 cut(s) 82, 95, 759
BspT107I GGYRCC 1 cut(s) 744
BssMI GATC 3 cut(s) 87, 407, 764
Bst2UI CCWGG 3 cut(s) 63, 402, 501
Bst4CI ACNGT 2 cut(s) 309, 323
BstBAI YACGTR 2 cut(s) 188, 883
BstDEI CTNAG 7 cut(s) 381, 387, 435, 486, 541, 770, 801
BstENI CCTNNNNNAGG 1 cut(s) 676
BstF5I GGATG 3 cut(s) 223, 397, 668
BstH2I RGCGCY 1 cut(s) 541
BstHHI GCGC 1 cut(s) 540
BstKTI GATC 3 cut(s) 90, 410, 767
BstMAI GTCTC 3 cut(s) 330, 377, 635
BstMBI GATC 3 cut(s) 87, 407, 764
BstNI CCWGG 3 cut(s) 63, 402, 501
BstNSI RCATGY 2 cut(s) 295, 813
BstSCI CCNGG 3 cut(s) 61, 400, 499
BstSFI CTRYAG 1 cut(s) 676
BstSNI TACGTA 1 cut(s) 188
BstV1I GCAGC 1 cut(s) 46
BstV2I GAAGAC 1 cut(s) 561
BstX2I RGATCY 1 cut(s) 87
BstYI RGATCY 1 cut(s) 87
BtsCI GGATG 3 cut(s) 223, 397, 668
CaiI CAGNNNCTG 1 cut(s) 548
CfoI GCGC 1 cut(s) 540
CseI GACGC 1 cut(s) 428
Csp6I GTAC 5 cut(s) 185, 305, 655, 745, 761
CspCI CAANNNNNGTGG 2 cut(s) 84, 119
CviAII CATG 3 cut(s) 292, 620, 810
CviJI RGCY 5 cut(s) 37, 66, 485, 632, 757
CviKI_1 RGCY 5 cut(s) 37, 66, 485, 632, 757
CviQI GTAC 5 cut(s) 185, 305, 655, 745, 761
DdeI CTNAG 7 cut(s) 381, 387, 435, 486, 541, 770, 801
DpnI GATC 3 cut(s) 89, 409, 766
DpnII GATC 3 cut(s) 87, 407, 764
DrdI GACNNNNNNGTC 1 cut(s) 563
DseDI GACNNNNNNGTC 1 cut(s) 563
Eco105I TACGTA 1 cut(s) 188
Eco32I GATATC 1 cut(s) 424
Eco47III AGCGCT 1 cut(s) 539
EcoNI CCTNNNNNAGG 1 cut(s) 676
EcoRII CCWGG 3 cut(s) 61, 400, 499
EcoRV GATATC 1 cut(s) 424
EcoT22I ATGCAT 1 cut(s) 297
FaeI CATG 3 cut(s) 295, 623, 813
FalI AAGNNNNNCTT 2 cut(s) 741, 773
FaqI GGGAC 2 cut(s) 426, 551
FatI CATG 3 cut(s) 291, 619, 809
FblI GTMKAC 1 cut(s) 505
Fnu4HI GCNGC 1 cut(s) 35
FokI GGATG 3 cut(s) 230, 384, 655
Fsp4HI GCNGC 1 cut(s) 35
FspBI CTAG 1 cut(s) 459
GlaI GCGC 1 cut(s) 539
GluI GCNGC 1 cut(s) 35
HaeII RGCGCY 1 cut(s) 541
HgaI GACGC 1 cut(s) 428
HhaI GCGC 1 cut(s) 540
Hin1II CATG 3 cut(s) 295, 623, 813
Hin6I GCGC 1 cut(s) 538
HinP1I GCGC 1 cut(s) 538
HindIII AAGCTT 1 cut(s) 755
HinfI GANTC 3 cut(s) 338, 545, 587
HphI GGTGA 2 cut(s) 16, 272
Hpy166II GTNNAC 3 cut(s) 506, 655, 880
Hpy188I TCNGA 7 cut(s) 130, 163, 421, 475, 544, 802, 833
Hpy188III TCNNGA 3 cut(s) 347, 459, 718
Hpy8I GTNNAC 3 cut(s) 506, 655, 880
Hpy99I CGWCG 1 cut(s) 531
HpyAV CCTTC 3 cut(s) 48, 278, 682
HpyCH4III ACNGT 2 cut(s) 309, 323
HpyCH4IV ACGT 2 cut(s) 187, 882
HpyCH4V TGCA 4 cut(s) 295, 593, 728, 809
HpyF3I CTNAG 7 cut(s) 381, 387, 435, 486, 541, 770, 801
HpySE526I ACGT 2 cut(s) 187, 882
Hsp92II CATG 3 cut(s) 295, 623, 813
HspAI GCGC 1 cut(s) 538
KpnI GGTACC 1 cut(s) 748
Kzo9I GATC 3 cut(s) 87, 407, 764
LmnI GCTCC 1 cut(s) 682
Lsp1109I GCAGC 1 cut(s) 46
LweI GCATC 2 cut(s) 406, 694
MaeI CTAG 1 cut(s) 459
MaeII ACGT 2 cut(s) 187, 882
MaeIII GTNAC 2 cut(s) 260, 622
MalI GATC 3 cut(s) 89, 409, 766
MboI GATC 3 cut(s) 87, 407, 764
MboII GAAGA 3 cut(s) 266, 566, 872
MflI RGATCY 1 cut(s) 87
MluCI AATT 4 cut(s) 469, 559, 723, 869
MlyI GAGTC 2 cut(s) 332, 539
MmeI TCCRAC 2 cut(s) 633, 811
MnlI CCTC 7 cut(s) 78, 159, 194, 405, 455, 577, 627
Mph1103I ATGCAT 1 cut(s) 297
MseI TTAA 2 cut(s) 236, 636
MspR9I CCNGG 3 cut(s) 63, 402, 501
MvaI CCWGG 3 cut(s) 63, 402, 501
NdeII GATC 3 cut(s) 87, 407, 764
NlaIII CATG 3 cut(s) 295, 623, 813
NlaIV GGNNCC 2 cut(s) 89, 746
NmuCI GTSAC 2 cut(s) 260, 622
NsiI ATGCAT 1 cut(s) 297
NspI RCATGY 2 cut(s) 295, 813
PfeI GAWTC 1 cut(s) 587
PflMI CCANNNNNTGG 1 cut(s) 795
PkrI GCNGC 1 cut(s) 36
PleI GAGTC 2 cut(s) 332, 539
PpsI GAGTC 2 cut(s) 332, 539
Ppu21I YACGTR 2 cut(s) 188, 883
PsiI TTATAA 2 cut(s) 113, 534
Psp6I CCWGG 3 cut(s) 61, 400, 499
PspGI CCWGG 3 cut(s) 61, 400, 499
PspN4I GGNNCC 2 cut(s) 89, 746
PstNI CAGNNNCTG 1 cut(s) 548
PsuI RGATCY 1 cut(s) 87
RsaI GTAC 5 cut(s) 186, 306, 656, 746, 762
RsaNI GTAC 5 cut(s) 185, 305, 655, 745, 761
SaqAI TTAA 2 cut(s) 236, 636
SatI GCNGC 1 cut(s) 35
Sau3AI GATC 3 cut(s) 87, 407, 764
SchI GAGTC 2 cut(s) 332, 539
ScrFI CCNGG 3 cut(s) 63, 402, 501
SfaNI GCATC 2 cut(s) 406, 694
SfcI CTRYAG 1 cut(s) 676
SnaBI TACGTA 1 cut(s) 188
Sse9I AATT 4 cut(s) 469, 559, 723, 869
SspMI CTAG 1 cut(s) 459
StyD4I CCNGG 3 cut(s) 61, 400, 499
TaaI ACNGT 2 cut(s) 309, 323
TaiI ACGT 2 cut(s) 190, 885
TaqI TCGA 3 cut(s) 149, 276, 410
TasI AATT 4 cut(s) 469, 559, 723, 869
TatI WGTACW 1 cut(s) 654
TfiI GAWTC 1 cut(s) 587
Tru1I TTAA 2 cut(s) 236, 636
Tru9I TTAA 2 cut(s) 236, 636
TseFI GTSAC 2 cut(s) 260, 622
TseI GCWGC 1 cut(s) 34
Tsp45I GTSAC 2 cut(s) 260, 622
TspDTI ATGAA 4 cut(s) 30, 147, 297, 720
TspGWI ACGGA 1 cut(s) 515
Van91I CCANNNNNTGG 1 cut(s) 795
XagI CCTNNNNNAGG 1 cut(s) 676
XbaI TCTAGA 1 cut(s) 458
XceI RCATGY 2 cut(s) 295, 813
XmiI GTMKAC 1 cut(s) 505
XspI CTAG 1 cut(s) 459
Zsp2I ATGCAT 1 cut(s) 297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.