Rw4G022670

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000235
Physical Location & Seq
Forward (+)
48113557 .. 48115422
1866 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G022670.1

Sequence Viewer

Length: 828 bp
ATGGCGAGAAGAGCTTGCACATCTCTTCCATTTCATGATGGTAGTGTTGAGGAGGAGAAGGGGCCTACCTTCTTCAAGATCATAAGGCCTGGATTCAACACTGAGCATTTGAGAATCCCAGCTGCATTTCGGAAGCATATATTACATGAATCCATTAAGAGGGCAACCTTGAAATTGAAGAATTCATCAGATACTTCCTGGACTGTTAAAGTAATTATAACAGGAAGGGATATATATTTGAAGGATGGCTGGCAGGAGTTTTTAAGAGATAACTCCCTAGGTGATTCCGAGTTCTTAGTGTTTAGGTATGACAAAAATATGCATTTCATCATTAATATTTTTGACAAAAATTGTGTTGAGAGAGTGAATATCACTGATCTCAGAACACATAAAGCACCCACCTTTCCAGACAATACTAAAGGGCCTTTGGCAAGACCACCTACAGCTGCAGGTGATCATCGATATGACTTGGCTGTGGAAGCTGCTCATGTGCTTAAATCCAAAGGCGAGCACCTTCCAAGTTATGCAGCTGCTGCAGCTTTCAAATCCAAATTTCCTTACTTTGCAAAAGTTCTTGCAGGATCGAAGATGGTGGCAATTCCCACACATTTCTATAGGGAGATATTTTCTTCAAGCAAATATGATGAACTTTTCCTGGTGACTGCAAAGGGAAAATTCACAGTGACGCTTCTCCCATGCACTGATACAGTGATGTTGTCTGGAGGGTGGTCTTCCTTTAGAAATGACAACCAACTCCAAGAAGATGATATTTGCATCTTTGAGCTTGTGAAAGAAAACACCATGGTGGTTCACATTTTCCGAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0007275 GO:0008150 GO:0009553 GO:0009566 GO:0009567 GO:0019953 GO:0022414 GO:0032501 GO:0032502 GO:0044703 GO:0048229 GO:0048856 GO:0051704
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

275

Amino Acids

31.34

Weight (kDa)

9.05

Isoelectric Point (pI)

45.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 24 - 117 9.7e-17 B3 DNA binding domain
B3 PF02362 195 - 275 2.1e-13 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000235)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g22700 FvH4_2g06920 FvH4_2g06920 FvH4_2g06960 FvH4_2g06960 FvH4_2g06960 FvH4_2g06960 FvH4_2g06970 FvH4_2g06970 FvH4_2g07230 FvH4_2g07231 FvH4_2g07231 FvH4_2g07240 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_4g19990 FvH4_4g19990 FvH4_4g19990 FvH4_4g20070
malus_domestica MD05G1072700.v1.1
prunus_persica Prupe.8G113100_v2.0.a1
pyrus_communis pycom05g06500 pycom10g06950
rosa_chinensis RchiOBHm_Chr4g0425711 RchiOBHm_Chr6g0259241 RchiOBHm_Chr6g0259251 RchiOBHm_Chr6g0259321 RchiOBHm_Chr6g0259331 RchiOBHm_Chr6g0259781 RchiOBHm_Chr6g0259791 RchiOBHm_Chr6g0259811 RchiOBHm_Chr6g0259881 RchiOBHm_Chr6g0259931
rosa_laevigata RLG00000007345 RLG00000014500 RLG00000014501 RLG00000014505 RLG00000014506 RLG00000014507 RLG00000014539 RLG00000014540 RLG00000014545 RLG00000014546
rosa_multiflora Rmu_co8137334.1_g000001 Rmu_co8454279.1_g000001 Rmu_co8476373.1_g000001 Rmu_sc0001078.1_g000001 Rmu_sc0003958.1_g000011 Rmu_sc0009049.1_g000001 Rmu_sc0009049.1_g000009 Rmu_sc0010546.1_g000001 Rmu_sc0011974.1_g000002 Rmu_sc0011974.1_g000003 Rmu_sc0033846.1_g000001 Rmu_sc0036999.1_g000001 Rmu_ssc0000052.1_g000015
rosa_roxburghii Rroxscaffold_5G00367690 Rroxscaffold_7G00206530 Rroxscaffold_7G00206540 Rroxscaffold_7G00206560 Rroxscaffold_7G00206570 Rroxscaffold_7G00207020 Rroxscaffold_7G00207030 Rroxscaffold_7G00207150 Rroxscaffold_7G00207160 Rroxscaffold_7G00207170 Rroxscaffold_7G00207270
rosa_rugosa Rorug04G0205300 Rorug05G0586300 Rorug05G0586400 Rorug05G0586500 Rorug05G0586600 Rorug05G0586700 Rorug05G0587300 Rorug05G0587400 Rorug05G0587500 Rorug05G0587600 Rorug05G0590500 Rorug05G0590600 Rorug05G0591200 Rorug05G0591300 Rorug05G0591400 Rorug05G0591500
rosa_samantha Rh4AG262200 Rh4BG268500 Rh4CG279700 Rh4DG264100 Rh6AG103100 Rh6AG103600 Rh6AG107100 Rh6AG107200 Rh6AG107700 Rh6BG095400 Rh6BG095500 Rh6BG095900 Rh6BG096000 Rh6BG096800 Rh6BG102600 Rh6BG102700 Rh6BG102800 Rh6BG103800 Rh6CG092300 Rh6CG092400 Rh6CG092800 Rh6CG092900 Rh6CG096200 Rh6CG096300 Rh6CG096800 Rh6CG097100 Rh6DG086300 Rh6DG086400 Rh6DG086700 Rh6DG086800 Rh6DG090000 Rh6DG090100 Rh6DG090500 Rh6DG090800
rosa_wichuraiana Rw4G022670 Rw6G008900 Rw6G008940 Rw6G009250 Rw6G009280 Rw6G009330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 218
AarI CACCTGC 1 cut(s) 440
Acc36I ACCTGC 1 cut(s) 440
AclWI GGATC 1 cut(s) 589
AcsI RAATTY 3 cut(s) 181, 551, 674
AfiI CCNNNNNNNGG 1 cut(s) 159
AgsI TTSAA 7 cut(s) 76, 97, 172, 178, 241, 544, 633
AjnI CCWGG 3 cut(s) 88, 197, 654
AleI CACNNNNGTG 1 cut(s) 803
AluBI AGCT 7 cut(s) 14, 122, 446, 482, 530, 539, 784
AluI AGCT 7 cut(s) 14, 122, 446, 482, 530, 539, 784
Alw21I GWGCWC 1 cut(s) 513
AlwI GGATC 1 cut(s) 589
AlwNI CAGNNNCTG 1 cut(s) 533
AoxI GGCC 3 cut(s) 62, 86, 422
ApeKI GCWGC 7 cut(s) 122, 446, 482, 527, 530, 533, 536
ApoI RAATTY 3 cut(s) 181, 551, 674
AseI ATTAAT 1 cut(s) 333
AspA2I CCTAGG 1 cut(s) 277
AspS9I GGNCC 2 cut(s) 62, 422
AsuHPI GGTGA 3 cut(s) 293, 464, 670
AvrII CCTAGG 1 cut(s) 277
BbsI GAAGAC 1 cut(s) 723
Bbv12I GWGCWC 1 cut(s) 513
BbvI GCAGC 7 cut(s) 109, 433, 469, 517, 520, 539, 548
BccI CCATC 3 cut(s) 32, 239, 583
BciT130I CCWGG 3 cut(s) 90, 199, 656
BclI TGATCA 1 cut(s) 454
BfaI CTAG 1 cut(s) 278
BfmI CTRYAG 4 cut(s) 441, 447, 534, 613
BfuAI ACCTGC 1 cut(s) 440
BisI GCNGC 7 cut(s) 123, 447, 483, 528, 531, 534, 537
BlnI CCTAGG 1 cut(s) 277
BlsI GCNGC 7 cut(s) 124, 448, 484, 529, 532, 535, 538
Bme1390I CCNGG 3 cut(s) 90, 199, 656
BmgT120I GGNCC 2 cut(s) 62, 422
BmiI GGNNCC 1 cut(s) 63
BmrFI CCNGG 3 cut(s) 90, 199, 656
BmsI GCATC 1 cut(s) 783
BpiI GAAGAC 1 cut(s) 723
BpmI CTGGAG 1 cut(s) 741
Bsa29I ATCGAT 1 cut(s) 460
BsaJI CCNNGG 2 cut(s) 277, 801
Bsc4I CCNNNNNNNGG 1 cut(s) 159
BseBI CCWGG 3 cut(s) 90, 199, 656
BseCI ATCGAT 1 cut(s) 460
BseDI CCNNGG 2 cut(s) 277, 801
BseGI GGATG 1 cut(s) 250
BseLI CCNNNNNNNGG 1 cut(s) 159
BseMII CTCAG 2 cut(s) 93, 394
BseRI GAGGAG 2 cut(s) 65, 68
BseXI GCAGC 7 cut(s) 109, 433, 469, 517, 520, 539, 548
BseYI CCCAGC 1 cut(s) 118
BshFI GGCC 3 cut(s) 64, 88, 424
BshVI ATCGAT 1 cut(s) 460
BsiHKAI GWGCWC 1 cut(s) 513
BslI CCNNNNNNNGG 1 cut(s) 159
BsnI GGCC 3 cut(s) 64, 88, 424
Bsp1286I GDGCHC 1 cut(s) 513
Bsp143I GATC 4 cut(s) 78, 376, 454, 581
Bsp19I CCATGG 1 cut(s) 801
BspANI GGCC 3 cut(s) 64, 88, 424
BspCNI CTCAG 2 cut(s) 94, 393
BspDI ATCGAT 1 cut(s) 460
BspHI TCATGA 1 cut(s) 34
BspLI GGNNCC 1 cut(s) 63
BspMAI CTGCAG 2 cut(s) 451, 538
BspMI ACCTGC 1 cut(s) 440
BspPI GGATC 1 cut(s) 589
BspQI GCTCTTC 1 cut(s) 4
BssECI CCNNGG 2 cut(s) 277, 801
BssMI GATC 4 cut(s) 78, 376, 454, 581
BssT1I CCWWGG 2 cut(s) 277, 801
Bst2UI CCWGG 3 cut(s) 90, 199, 656
Bst4CI ACNGT 3 cut(s) 205, 682, 709
Bst6I CTCTTC 2 cut(s) 4, 30
BstAPI GCANNNNNTGC 1 cut(s) 533
BstC8I GCNNGC 3 cut(s) 16, 251, 509
BstDEI CTNAG 3 cut(s) 102, 295, 380
BstDSI CCRYGG 1 cut(s) 801
BstF5I GGATG 1 cut(s) 250
BstKTI GATC 4 cut(s) 81, 379, 457, 584
BstMBI GATC 4 cut(s) 78, 376, 454, 581
BstMWI GCNNNNNNNGC 4 cut(s) 11, 479, 533, 536
BstNI CCWGG 3 cut(s) 90, 199, 656
BstSCI CCNGG 3 cut(s) 88, 197, 654
BstSFI CTRYAG 4 cut(s) 441, 447, 534, 613
BstV1I GCAGC 7 cut(s) 109, 433, 469, 517, 520, 539, 548
BstV2I GAAGAC 1 cut(s) 723
Bsu15I ATCGAT 1 cut(s) 460
BsuRI GGCC 3 cut(s) 64, 88, 424
BsuTUI ATCGAT 1 cut(s) 460
BtgI CCRYGG 1 cut(s) 801
BtsCI GGATG 1 cut(s) 250
BtsIMutI CAGTG 5 cut(s) 99, 372, 687, 699, 714
BveI ACCTGC 1 cut(s) 440
Cac8I GCNNGC 3 cut(s) 16, 251, 509
CaiI CAGNNNCTG 1 cut(s) 533
CciI TCATGA 1 cut(s) 34
Cfr13I GGNCC 2 cut(s) 62, 422
ClaI ATCGAT 1 cut(s) 460
CseI GACGC 1 cut(s) 694
CviAII CATG 5 cut(s) 35, 146, 488, 696, 802
DdeI CTNAG 3 cut(s) 102, 295, 380
DpnI GATC 4 cut(s) 80, 378, 456, 583
DpnII GATC 4 cut(s) 78, 376, 454, 581
Eam1104I CTCTTC 2 cut(s) 4, 30
EarI CTCTTC 2 cut(s) 4, 30
Eco130I CCWWGG 2 cut(s) 277, 801
Eco147I AGGCCT 1 cut(s) 88
EcoO109I RGGNCCY 2 cut(s) 62, 422
EcoRI GAATTC 1 cut(s) 181
EcoRII CCWGG 3 cut(s) 88, 197, 654
EcoT14I CCWWGG 2 cut(s) 277, 801
EcoT22I ATGCAT 1 cut(s) 324
ErhI CCWWGG 2 cut(s) 277, 801
FaeI CATG 5 cut(s) 38, 149, 491, 699, 805
FatI CATG 5 cut(s) 34, 145, 487, 695, 801
FbaI TGATCA 1 cut(s) 454
Fnu4HI GCNGC 7 cut(s) 123, 447, 483, 528, 531, 534, 537
FokI GGATG 1 cut(s) 257
Fsp4HI GCNGC 7 cut(s) 123, 447, 483, 528, 531, 534, 537
FspBI CTAG 1 cut(s) 278
GluI GCNGC 7 cut(s) 123, 447, 483, 528, 531, 534, 537
GsaI CCCAGC 1 cut(s) 122
GsuI CTGGAG 1 cut(s) 741
HaeIII GGCC 3 cut(s) 64, 88, 424
HgaI GACGC 1 cut(s) 694
Hin1II CATG 5 cut(s) 38, 149, 491, 699, 805
HinfI GANTC 4 cut(s) 93, 114, 149, 284
HphI GGTGA 3 cut(s) 293, 464, 670
Hpy166II GTNNAC 1 cut(s) 811
Hpy188I TCNGA 5 cut(s) 132, 190, 289, 383, 821
Hpy188III TCNNGA 4 cut(s) 35, 76, 407, 720
Hpy8I GTNNAC 1 cut(s) 811
HpyAV CCTTC 5 cut(s) 52, 79, 219, 235, 524
HpyCH4III ACNGT 3 cut(s) 205, 682, 709
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 479, 533, 536
HpyF3I CTNAG 3 cut(s) 102, 295, 380
Hsp92II CATG 5 cut(s) 38, 149, 491, 699, 805
Ksp22I TGATCA 1 cut(s) 454
Kzo9I GATC 4 cut(s) 78, 376, 454, 581
LguI GCTCTTC 1 cut(s) 4
Lsp1109I GCAGC 7 cut(s) 109, 433, 469, 517, 520, 539, 548
LweI GCATC 1 cut(s) 783
MaeI CTAG 1 cut(s) 278
MaeIII GTNAC 2 cut(s) 658, 682
MalI GATC 4 cut(s) 80, 378, 456, 583
MboI GATC 4 cut(s) 78, 376, 454, 581
MboII GAAGA 8 cut(s) 17, 21, 64, 190, 598, 621, 723, 773
MhlI GDGCHC 1 cut(s) 513
MluCI AATT 7 cut(s) 173, 181, 213, 349, 551, 597, 674
MnlI CCTC 4 cut(s) 43, 46, 153, 716
Mph1103I ATGCAT 1 cut(s) 324
MseI TTAA 5 cut(s) 156, 207, 263, 333, 495
MslI CAYNNNNRTG 2 cut(s) 462, 803
MspA1I CMGCKG 3 cut(s) 122, 446, 530
MspR9I CCNGG 3 cut(s) 90, 199, 656
MvaI CCWGG 3 cut(s) 90, 199, 656
MwoI GCNNNNNNNGC 4 cut(s) 11, 479, 533, 536
NcoI CCATGG 1 cut(s) 801
NdeII GATC 4 cut(s) 78, 376, 454, 581
NlaIII CATG 5 cut(s) 38, 149, 491, 699, 805
NlaIV GGNNCC 1 cut(s) 63
NmuCI GTSAC 2 cut(s) 658, 682
NsiI ATGCAT 1 cut(s) 324
OliI CACNNNNGTG 1 cut(s) 803
PagI TCATGA 1 cut(s) 34
PaqCI CACCTGC 1 cut(s) 440
PceI AGGCCT 1 cut(s) 88
PciSI GCTCTTC 1 cut(s) 4
PfeI GAWTC 4 cut(s) 93, 114, 149, 284
PfoI TCCNGGA 1 cut(s) 197
PkrI GCNGC 7 cut(s) 124, 448, 484, 529, 532, 535, 538
PshBI ATTAAT 1 cut(s) 333
PsiI TTATAA 1 cut(s) 218
Psp6I CCWGG 3 cut(s) 88, 197, 654
PspFI CCCAGC 1 cut(s) 118
PspGI CCWGG 3 cut(s) 88, 197, 654
PspN4I GGNNCC 1 cut(s) 63
PspPI GGNCC 2 cut(s) 62, 422
PstI CTGCAG 2 cut(s) 451, 538
PstNI CAGNNNCTG 1 cut(s) 533
PvuII CAGCTG 3 cut(s) 122, 446, 530
RseI CAYNNNNRTG 2 cut(s) 462, 803
SapI GCTCTTC 1 cut(s) 4
SaqAI TTAA 5 cut(s) 156, 207, 263, 333, 495
SatI GCNGC 7 cut(s) 123, 447, 483, 528, 531, 534, 537
Sau3AI GATC 4 cut(s) 78, 376, 454, 581
Sau96I GGNCC 2 cut(s) 62, 422
ScrFI CCNGG 3 cut(s) 90, 199, 656
SduI GDGCHC 1 cut(s) 513
SfaNI GCATC 1 cut(s) 783
SfcI CTRYAG 4 cut(s) 441, 447, 534, 613
SmiMI CAYNNNNRTG 2 cut(s) 462, 803
Sse9I AATT 7 cut(s) 173, 181, 213, 349, 551, 597, 674
SseBI AGGCCT 1 cut(s) 88
SspI AATATT 1 cut(s) 337
SspMI CTAG 1 cut(s) 278
StuI AGGCCT 1 cut(s) 88
StyD4I CCNGG 3 cut(s) 88, 197, 654
StyI CCWWGG 2 cut(s) 277, 801
TaaI ACNGT 3 cut(s) 205, 682, 709
TaqI TCGA 2 cut(s) 460, 584
TasI AATT 7 cut(s) 173, 181, 213, 349, 551, 597, 674
TfiI GAWTC 4 cut(s) 93, 114, 149, 284
Tru1I TTAA 5 cut(s) 156, 207, 263, 333, 495
Tru9I TTAA 5 cut(s) 156, 207, 263, 333, 495
TscAI CASTG 5 cut(s) 106, 379, 687, 706, 714
TseFI GTSAC 2 cut(s) 658, 682
TseI GCWGC 7 cut(s) 122, 446, 482, 527, 530, 533, 536
Tsp45I GTSAC 2 cut(s) 658, 682
TspDTI ATGAA 5 cut(s) 23, 162, 174, 316, 660
TspRI CASTG 5 cut(s) 106, 379, 687, 706, 714
VspI ATTAAT 1 cut(s) 333
XapI RAATTY 3 cut(s) 181, 551, 674
XmaJI CCTAGG 1 cut(s) 277
XspI CTAG 1 cut(s) 278
Zsp2I ATGCAT 1 cut(s) 324
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.