FvH4_2g07300

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000235
Physical Location & Seq
Forward (+)
6030866 .. 6034242
3377 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g07300.t9

Sequence Viewer

Length: 876 bp
ATGGTGAGACCCAAGAATGGTATCAAGGAAAAAAGGCCTTCATTTTTCAAGGTTATTATTCCAGGCTACTCCACTCGATATTTGAGGATCCCACCACCGTTCTTGGAGCTTATAAGTAATGACCTATCTGGTGAAGCAACATTGAACTCCAACACTTGTTCAGAGTGGTCCTGGAATGTTGGAGTAACTAAAACAATACGAAGTGTGTATTTCAAGGATGGTTGGCAGGAGTTCCTAAAAGATAACTCTGTGGAAGATGGCGACTTTGTGGTATTCGAGTATGAAGGAAACATGAATTTCAATGTACAGATTTTTGATAACAGTGGTTGTGAACGAGACTGTTTTCCTAACATCGGAACACCCAAAATATCCTCTTTTGCTGAGAAGAAAAGGCATCCTAGTACACCGAGGAAATCAGATATTTGTTGTTCTAAGTGTCACCTCCAGCTGAACTCTAGAAAAGACAGTTCAGATAACATAGCTAAGCAAAGGCTTCCTGGTAGGCCTAGAAAATCCCATATCGGTTGTTCTAAGCGGCACCCTTGCCCATCGAACTTTAGAAAAGTCAGTGCAGAATCTTTCAAGTCCAACTATCCTCATGTCACATTAGCCATTAAAAAGACTTATTGTGTGTACTCATTCCCCACATCCTTCTATAAGGAGCATCTCTCTCCAGGCAATTATGAAGTTGTATTCCTGAAAATTGCAGAAGGGAAAAAATGGTACAAAGTGAAGCTTGTAAGATCGATCGCAACAGCCTTCATGTCGAAAGGGTGGTCTAAGTTTGCACGTGCTAATCAAATCAAGGTCGGAAATGTTTGTGCTTTTGAAGTTGTGGAAGAAAACAAATTGGTGGTTCACATAATCCAGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0007275 GO:0008150 GO:0009553 GO:0009566 GO:0009567 GO:0019953 GO:0022414 GO:0032501 GO:0032502 GO:0044703 GO:0048229 GO:0048856 GO:0051704
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

292

Amino Acids

33.32

Weight (kDa)

9.49

Isoelectric Point (pI)

35.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 15 - 107 4e-17 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000235)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g22700 FvH4_2g06920 FvH4_2g06920 FvH4_2g06960 FvH4_2g06960 FvH4_2g06960 FvH4_2g06960 FvH4_2g06970 FvH4_2g06970 FvH4_2g07230 FvH4_2g07231 FvH4_2g07231 FvH4_2g07240 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_4g19990 FvH4_4g19990 FvH4_4g19990 FvH4_4g20070
malus_domestica MD05G1072700.v1.1
prunus_persica Prupe.8G113100_v2.0.a1
pyrus_communis pycom05g06500 pycom10g06950
rosa_chinensis RchiOBHm_Chr4g0425711 RchiOBHm_Chr6g0259241 RchiOBHm_Chr6g0259251 RchiOBHm_Chr6g0259321 RchiOBHm_Chr6g0259331 RchiOBHm_Chr6g0259781 RchiOBHm_Chr6g0259791 RchiOBHm_Chr6g0259811 RchiOBHm_Chr6g0259881 RchiOBHm_Chr6g0259931
rosa_laevigata RLG00000007345 RLG00000014500 RLG00000014501 RLG00000014505 RLG00000014506 RLG00000014507 RLG00000014539 RLG00000014540 RLG00000014545 RLG00000014546
rosa_multiflora Rmu_co8137334.1_g000001 Rmu_co8454279.1_g000001 Rmu_co8476373.1_g000001 Rmu_sc0001078.1_g000001 Rmu_sc0003958.1_g000011 Rmu_sc0009049.1_g000001 Rmu_sc0009049.1_g000009 Rmu_sc0010546.1_g000001 Rmu_sc0011974.1_g000002 Rmu_sc0011974.1_g000003 Rmu_sc0033846.1_g000001 Rmu_sc0036999.1_g000001 Rmu_ssc0000052.1_g000015
rosa_roxburghii Rroxscaffold_5G00367690 Rroxscaffold_7G00206530 Rroxscaffold_7G00206540 Rroxscaffold_7G00206560 Rroxscaffold_7G00206570 Rroxscaffold_7G00207020 Rroxscaffold_7G00207030 Rroxscaffold_7G00207150 Rroxscaffold_7G00207160 Rroxscaffold_7G00207170 Rroxscaffold_7G00207270
rosa_rugosa Rorug04G0205300 Rorug05G0586300 Rorug05G0586400 Rorug05G0586500 Rorug05G0586600 Rorug05G0586700 Rorug05G0587300 Rorug05G0587400 Rorug05G0587500 Rorug05G0587600 Rorug05G0590500 Rorug05G0590600 Rorug05G0591200 Rorug05G0591300 Rorug05G0591400 Rorug05G0591500
rosa_samantha Rh4AG262200 Rh4BG268500 Rh4CG279700 Rh4DG264100 Rh6AG103100 Rh6AG103600 Rh6AG107100 Rh6AG107200 Rh6AG107700 Rh6BG095400 Rh6BG095500 Rh6BG095900 Rh6BG096000 Rh6BG096800 Rh6BG102600 Rh6BG102700 Rh6BG102800 Rh6BG103800 Rh6CG092300 Rh6CG092400 Rh6CG092800 Rh6CG092900 Rh6CG096200 Rh6CG096300 Rh6CG096800 Rh6CG097100 Rh6DG086300 Rh6DG086400 Rh6DG086700 Rh6DG086800 Rh6DG090000 Rh6DG090100 Rh6DG090500 Rh6DG090800
rosa_wichuraiana Rw4G022670 Rw6G008900 Rw6G008940 Rw6G009250 Rw6G009280 Rw6G009330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 113
AccB1I GGYRCC 1 cut(s) 537
AciI CCGC 1 cut(s) 535
AclWI GGATC 2 cut(s) 82, 95
AcsI RAATTY 1 cut(s) 295
AcvI CACGTG 1 cut(s) 791
AfaI GTAC 4 cut(s) 306, 403, 635, 725
AfiI CCNNNNNNNGG 2 cut(s) 17, 353
AgsI TTSAA 6 cut(s) 49, 145, 214, 301, 583, 830
AjnI CCWGG 5 cut(s) 61, 170, 496, 673, 867
AluBI AGCT 4 cut(s) 109, 448, 482, 736
AluI AGCT 4 cut(s) 109, 448, 482, 736
Alw26I GTCTC 1 cut(s) 330
AlwI GGATC 2 cut(s) 82, 95
AoxI GGCC 2 cut(s) 35, 503
ApoI RAATTY 1 cut(s) 295
ArsI GACNNNNNNTTYG 2 cut(s) 761, 793
AspS9I GGNCC 1 cut(s) 168
AsuHPI GGTGA 3 cut(s) 16, 143, 431
AvaII GGWCC 1 cut(s) 168
BamHI GGATCC 1 cut(s) 87
BanI GGYRCC 1 cut(s) 537
BbrPI CACGTG 1 cut(s) 791
BccI CCATC 3 cut(s) 212, 251, 556
BciT130I CCWGG 5 cut(s) 63, 172, 498, 675, 869
BcoDI GTCTC 1 cut(s) 330
BfaI CTAG 3 cut(s) 399, 456, 507
BisI GCNGC 1 cut(s) 536
BlpI GCTNAGC 1 cut(s) 483
BlsI GCNGC 1 cut(s) 537
Bme1390I CCNGG 5 cut(s) 63, 172, 498, 675, 869
Bme18I GGWCC 1 cut(s) 168
BmgT120I GGNCC 1 cut(s) 168
BmiI GGNNCC 2 cut(s) 89, 539
BmrFI CCNGG 5 cut(s) 63, 172, 498, 675, 869
BmsI GCATC 2 cut(s) 403, 673
BplI GAGNNNNNCTC 2 cut(s) 653, 685
BpmI CTGGAG 2 cut(s) 428, 657
Bpu1102I GCTNAGC 1 cut(s) 483
Bsa29I ATCGAT 1 cut(s) 746
BsaAI YACGTR 1 cut(s) 791
BsaJI CCNNGG 1 cut(s) 407
Bsc4I CCNNNNNNNGG 2 cut(s) 17, 353
BseBI CCWGG 5 cut(s) 63, 172, 498, 675, 869
BseCI ATCGAT 1 cut(s) 746
BseDI CCNNGG 1 cut(s) 407
BseGI GGATG 3 cut(s) 223, 394, 647
BseLI CCNNNNNNNGG 2 cut(s) 17, 353
BseMII CTCAG 1 cut(s) 372
BsgI GTGCAG 1 cut(s) 591
Bsh1285I CGRYCG 1 cut(s) 750
BshFI GGCC 2 cut(s) 37, 505
BshNI GGYRCC 1 cut(s) 537
BshVI ATCGAT 1 cut(s) 746
BsiEI CGRYCG 1 cut(s) 750
BslI CCNNNNNNNGG 2 cut(s) 17, 353
BsmAI GTCTC 1 cut(s) 330
BsnI GGCC 2 cut(s) 37, 505
Bsp1407I TGTACA 1 cut(s) 304
Bsp143I GATC 3 cut(s) 87, 743, 747
Bsp1720I GCTNAGC 1 cut(s) 483
BspACI CCGC 1 cut(s) 535
BspANI GGCC 2 cut(s) 37, 505
BspCNI CTCAG 1 cut(s) 373
BspDI ATCGAT 1 cut(s) 746
BspLI GGNNCC 2 cut(s) 89, 539
BspPI GGATC 2 cut(s) 82, 95
BspT107I GGYRCC 1 cut(s) 537
BsrGI TGTACA 1 cut(s) 304
BssECI CCNNGG 1 cut(s) 407
BssMI GATC 3 cut(s) 87, 743, 747
Bst2UI CCWGG 5 cut(s) 63, 172, 498, 675, 869
Bst4CI ACNGT 4 cut(s) 99, 323, 341, 467
BstAUI TGTACA 1 cut(s) 304
BstBAI YACGTR 1 cut(s) 791
BstDEI CTNAG 5 cut(s) 381, 432, 483, 531, 780
BstF5I GGATG 3 cut(s) 223, 394, 647
BstKTI GATC 3 cut(s) 90, 746, 750
BstMAI GTCTC 1 cut(s) 330
BstMBI GATC 3 cut(s) 87, 743, 747
BstMCI CGRYCG 1 cut(s) 750
BstNI CCWGG 5 cut(s) 63, 172, 498, 675, 869
BstSCI CCNGG 5 cut(s) 61, 170, 496, 673, 867
BstX2I RGATCY 1 cut(s) 87
BstYI RGATCY 1 cut(s) 87
Bsu15I ATCGAT 1 cut(s) 746
BsuRI GGCC 2 cut(s) 37, 505
BsuTUI ATCGAT 1 cut(s) 746
BtsCI GGATG 3 cut(s) 223, 394, 647
BtsIMutI CAGTG 2 cut(s) 328, 574
Cfr13I GGNCC 1 cut(s) 168
ClaI ATCGAT 1 cut(s) 746
Csp6I GTAC 4 cut(s) 305, 402, 634, 724
CspCI CAANNNNNGTGG 2 cut(s) 84, 119
CviAII CATG 3 cut(s) 292, 599, 763
CviQI GTAC 4 cut(s) 305, 402, 634, 724
DdeI CTNAG 5 cut(s) 381, 432, 483, 531, 780
DpnI GATC 3 cut(s) 89, 745, 749
DpnII GATC 3 cut(s) 87, 743, 747
Eco147I AGGCCT 2 cut(s) 37, 505
Eco47I GGWCC 1 cut(s) 168
Eco72I CACGTG 1 cut(s) 791
EcoRII CCWGG 5 cut(s) 61, 170, 496, 673, 867
FaeI CATG 3 cut(s) 295, 602, 766
FalI AAGNNNNNCTT 2 cut(s) 720, 752
FatI CATG 3 cut(s) 291, 598, 762
Fnu4HI GCNGC 1 cut(s) 536
FokI GGATG 3 cut(s) 230, 381, 634
Fsp4HI GCNGC 1 cut(s) 536
FspBI CTAG 3 cut(s) 399, 456, 507
GluI GCNGC 1 cut(s) 536
GsuI CTGGAG 2 cut(s) 428, 657
HaeIII GGCC 2 cut(s) 37, 505
Hin1II CATG 3 cut(s) 295, 602, 766
HindIII AAGCTT 1 cut(s) 734
HinfI GANTC 1 cut(s) 575
HphI GGTGA 3 cut(s) 16, 143, 431
Hpy166II GTNNAC 4 cut(s) 332, 404, 634, 859
Hpy188I TCNGA 5 cut(s) 163, 356, 418, 472, 812
Hpy188III TCNNGA 2 cut(s) 456, 697
Hpy8I GTNNAC 4 cut(s) 332, 404, 634, 859
HpyAV CCTTC 5 cut(s) 48, 278, 661, 704, 769
HpyCH4III ACNGT 4 cut(s) 99, 323, 341, 467
HpyCH4IV ACGT 1 cut(s) 790
HpyCH4V TGCA 3 cut(s) 572, 707, 788
HpyF3I CTNAG 5 cut(s) 381, 432, 483, 531, 780
HpySE526I ACGT 1 cut(s) 790
Hsp92II CATG 3 cut(s) 295, 602, 766
Kzo9I GATC 3 cut(s) 87, 743, 747
LmnI GCTCC 2 cut(s) 106, 661
LweI GCATC 2 cut(s) 403, 673
MaeI CTAG 3 cut(s) 399, 456, 507
MaeII ACGT 1 cut(s) 790
MaeIII GTNAC 3 cut(s) 184, 437, 601
MalI GATC 3 cut(s) 89, 745, 749
MboI GATC 3 cut(s) 87, 743, 747
MboII GAAGA 3 cut(s) 266, 397, 851
MflI RGATCY 1 cut(s) 87
MluCI AATT 4 cut(s) 295, 679, 702, 848
MmeI TCCRAC 4 cut(s) 160, 174, 612, 790
MnlI CCTC 5 cut(s) 78, 382, 402, 452, 606
MseI TTAA 1 cut(s) 615
MspA1I CMGCKG 1 cut(s) 448
MspR9I CCNGG 5 cut(s) 63, 172, 498, 675, 869
MvaI CCWGG 5 cut(s) 63, 172, 498, 675, 869
NdeII GATC 3 cut(s) 87, 743, 747
NlaIII CATG 3 cut(s) 295, 602, 766
NlaIV GGNNCC 2 cut(s) 89, 539
NmuCI GTSAC 2 cut(s) 437, 601
PceI AGGCCT 2 cut(s) 37, 505
PfeI GAWTC 1 cut(s) 575
PfoI TCCNGGA 2 cut(s) 170, 867
PkrI GCNGC 1 cut(s) 537
Ple19I CGATCG 1 cut(s) 750
PmaCI CACGTG 1 cut(s) 791
PmlI CACGTG 1 cut(s) 791
Ppu21I YACGTR 1 cut(s) 791
PsiI TTATAA 1 cut(s) 113
Psp6I CCWGG 5 cut(s) 61, 170, 496, 673, 867
PspCI CACGTG 1 cut(s) 791
PspGI CCWGG 5 cut(s) 61, 170, 496, 673, 867
PspN4I GGNNCC 2 cut(s) 89, 539
PspPI GGNCC 1 cut(s) 168
PsuI RGATCY 1 cut(s) 87
PvuI CGATCG 1 cut(s) 750
PvuII CAGCTG 1 cut(s) 448
RsaI GTAC 4 cut(s) 306, 403, 635, 725
RsaNI GTAC 4 cut(s) 305, 402, 634, 724
SaqAI TTAA 1 cut(s) 615
SatI GCNGC 1 cut(s) 536
Sau3AI GATC 3 cut(s) 87, 743, 747
Sau96I GGNCC 1 cut(s) 168
ScrFI CCNGG 5 cut(s) 63, 172, 498, 675, 869
SetI ASST 9 cut(s) 54, 111, 126, 444, 450, 484, 738, 793, 810
SfaNI GCATC 2 cut(s) 403, 673
SinI GGWCC 1 cut(s) 168
Sse9I AATT 4 cut(s) 295, 679, 702, 848
SseBI AGGCCT 2 cut(s) 37, 505
SsiI CCGC 1 cut(s) 535
SspMI CTAG 3 cut(s) 399, 456, 507
StuI AGGCCT 2 cut(s) 37, 505
StyD4I CCNGG 5 cut(s) 61, 170, 496, 673, 867
TaaI ACNGT 4 cut(s) 99, 323, 341, 467
TaiI ACGT 1 cut(s) 793
TaqI TCGA 5 cut(s) 76, 276, 551, 746, 767
TasI AATT 4 cut(s) 295, 679, 702, 848
TatI WGTACW 3 cut(s) 304, 401, 633
TauI GCSGC 1 cut(s) 538
TfiI GAWTC 1 cut(s) 575
Tru1I TTAA 1 cut(s) 615
Tru9I TTAA 1 cut(s) 615
TscAI CASTG 2 cut(s) 328, 574
TseFI GTSAC 2 cut(s) 437, 601
Tsp45I GTSAC 2 cut(s) 437, 601
TspDTI ATGAA 5 cut(s) 30, 297, 308, 699, 751
TspRI CASTG 2 cut(s) 328, 574
VpaK11BI GGWCC 1 cut(s) 168
XapI RAATTY 1 cut(s) 295
XbaI TCTAGA 1 cut(s) 455
XspI CTAG 3 cut(s) 399, 456, 507
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.