RLG00000014500

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0000235
Physical Location & Seq
Reverse (-)
54019669 .. 54021652
1984 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014500

Sequence Viewer

Length: 804 bp
ATGGCGAGAACAACATTCAACAATGGTGGTATGGAGGAAAAAAAGGGCCCTACGTTCTTCAAGATCGTAAAAACCGGTTTCAACACTGAGGATTTGAGAATCCCACCCAAATTCCGGAGACACCTGTTGAATGAGCTCTCCGGCAGGGCAACTTTGAATTTAATGGATTCTTCAAATAGTTCATGGACTGTTGATGTGAGCCAAACAGAAGGGGAGATATATTTCAAGAGTGGATGGCAGAAGTTTGTAAGAGATAACTCCTTAGGTAACTTTGAATTCTTAGTATTTTGGTATGAGAGCGATATGCAATTTAGCATTGAGATCTTTGACAGAAATGCGAGTAAGAGGATCACGTTTCCAGATGTCAGAACACACAGACGAGGTGAAAATTCAGCTGCAGGTATACACACTCAAAGTTGTCAATCCAACAAACTCAAAGAGCTTAAAGAAGAGGAGGAAGATTACCAAGATAACAAAGAAGAAGAAGCTGCCACATTGCACAATTTGGAAGGCCCTGCGTTTACAAGCATTGTTGGACAAAACTTTTATAATTTGGCAGTTCCTACAGACTTTTCTATGAACTACTTTCCTCTGGGATACTACACCATTGTCCTGAAAAATTCAGAGGCAAGAGAATGGGAAGTAAGCGTTGTGCCTAATGGGAAGGATGCTTCCTGTAATACGGTTAAACTTTCTGCAGGATGGGCTGCTTTTAGACGTGACAACCAAATCAACTCCGGAGATACTTGCACATTTGAGCTTGTGGAAAGCAAAACAATGTTGGTTCACATTGATCCTAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0007275 GO:0008150 GO:0009553 GO:0009566 GO:0009567 GO:0019953 GO:0022414 GO:0032501 GO:0032502 GO:0044703 GO:0048229 GO:0048856 GO:0051704
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

268

Amino Acids

30.55

Weight (kDa)

5.4

Isoelectric Point (pI)

44.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 19 - 112 8e-16 B3 DNA binding domain
B3 PF02362 185 - 264 1e-11 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000235)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g22700 FvH4_2g06920 FvH4_2g06920 FvH4_2g06960 FvH4_2g06960 FvH4_2g06960 FvH4_2g06960 FvH4_2g06970 FvH4_2g06970 FvH4_2g07230 FvH4_2g07231 FvH4_2g07231 FvH4_2g07240 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_4g19990 FvH4_4g19990 FvH4_4g19990 FvH4_4g20070
malus_domestica MD05G1072700.v1.1
prunus_persica Prupe.8G113100_v2.0.a1
pyrus_communis pycom05g06500 pycom10g06950
rosa_chinensis RchiOBHm_Chr4g0425711 RchiOBHm_Chr6g0259241 RchiOBHm_Chr6g0259251 RchiOBHm_Chr6g0259321 RchiOBHm_Chr6g0259331 RchiOBHm_Chr6g0259781 RchiOBHm_Chr6g0259791 RchiOBHm_Chr6g0259811 RchiOBHm_Chr6g0259881 RchiOBHm_Chr6g0259931
rosa_laevigata RLG00000007345 RLG00000014500 RLG00000014501 RLG00000014505 RLG00000014506 RLG00000014507 RLG00000014539 RLG00000014540 RLG00000014545 RLG00000014546
rosa_multiflora Rmu_co8137334.1_g000001 Rmu_co8454279.1_g000001 Rmu_co8476373.1_g000001 Rmu_sc0001078.1_g000001 Rmu_sc0003958.1_g000011 Rmu_sc0009049.1_g000001 Rmu_sc0009049.1_g000009 Rmu_sc0010546.1_g000001 Rmu_sc0011974.1_g000002 Rmu_sc0011974.1_g000003 Rmu_sc0033846.1_g000001 Rmu_sc0036999.1_g000001 Rmu_ssc0000052.1_g000015
rosa_roxburghii Rroxscaffold_5G00367690 Rroxscaffold_7G00206530 Rroxscaffold_7G00206540 Rroxscaffold_7G00206560 Rroxscaffold_7G00206570 Rroxscaffold_7G00207020 Rroxscaffold_7G00207030 Rroxscaffold_7G00207150 Rroxscaffold_7G00207160 Rroxscaffold_7G00207170 Rroxscaffold_7G00207270
rosa_rugosa Rorug04G0205300 Rorug05G0586300 Rorug05G0586400 Rorug05G0586500 Rorug05G0586600 Rorug05G0586700 Rorug05G0587300 Rorug05G0587400 Rorug05G0587500 Rorug05G0587600 Rorug05G0590500 Rorug05G0590600 Rorug05G0591200 Rorug05G0591300 Rorug05G0591400 Rorug05G0591500
rosa_samantha Rh4AG262200 Rh4BG268500 Rh4CG279700 Rh4DG264100 Rh6AG103100 Rh6AG103600 Rh6AG107100 Rh6AG107200 Rh6AG107700 Rh6BG095400 Rh6BG095500 Rh6BG095900 Rh6BG096000 Rh6BG096800 Rh6BG102600 Rh6BG102700 Rh6BG102800 Rh6BG103800 Rh6CG092300 Rh6CG092400 Rh6CG092800 Rh6CG092900 Rh6CG096200 Rh6CG096300 Rh6CG096800 Rh6CG097100 Rh6DG086300 Rh6DG086400 Rh6DG086700 Rh6DG086800 Rh6DG090000 Rh6DG090100 Rh6DG090500 Rh6DG090800
rosa_wichuraiana Rw4G022670 Rw6G008900 Rw6G008940 Rw6G009250 Rw6G009280 Rw6G009330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 549
Acc36I ACCTGC 1 cut(s) 389
AccI GTMKAC 1 cut(s) 403
AccIII TCCGGA 2 cut(s) 114, 737
AclWI GGATC 2 cut(s) 356, 788
AcsI RAATTY 5 cut(s) 110, 157, 275, 388, 619
AfiI CCNNNNNNNGG 1 cut(s) 114
AgeI ACCGGT 1 cut(s) 74
AgsI TTSAA 8 cut(s) 19, 61, 82, 130, 157, 174, 226, 275
AjiI CACGTC 1 cut(s) 719
AluBI AGCT 5 cut(s) 136, 395, 442, 488, 760
AluI AGCT 5 cut(s) 136, 395, 442, 488, 760
Alw21I GWGCWC 1 cut(s) 138
Alw26I GTCTC 1 cut(s) 112
AlwI GGATC 2 cut(s) 356, 788
Aor13HI TCCGGA 2 cut(s) 114, 737
AoxI GGCC 2 cut(s) 46, 511
ApaI GGGCCC 1 cut(s) 50
ApeKI GCWGC 3 cut(s) 395, 488, 707
ApoI RAATTY 5 cut(s) 110, 157, 275, 388, 619
AsiGI ACCGGT 1 cut(s) 74
AspS9I GGNCC 3 cut(s) 46, 47, 512
AsuHPI GGTGA 1 cut(s) 395
AxyI CCTNAGG 1 cut(s) 262
BaeGI GKGCMC 1 cut(s) 50
BanII GRGCYC 2 cut(s) 50, 138
Bbv12I GWGCWC 1 cut(s) 138
BbvI GCAGC 3 cut(s) 382, 475, 694
BccI CCATC 2 cut(s) 228, 696
BciVI GTATCC 1 cut(s) 590
BcoDI GTCTC 1 cut(s) 112
BfmI CTRYAG 3 cut(s) 396, 564, 696
BfuAI ACCTGC 1 cut(s) 389
BfuI GTATCC 1 cut(s) 590
BglII AGATCT 1 cut(s) 321
BisI GCNGC 3 cut(s) 396, 489, 708
BlsI GCNGC 3 cut(s) 397, 490, 709
BmgBI CACGTC 1 cut(s) 719
BmgT120I GGNCC 3 cut(s) 46, 47, 512
BmiI GGNNCC 1 cut(s) 48
BmsI GCATC 1 cut(s) 658
BsaWI WCCGGW 3 cut(s) 74, 114, 737
Bsc4I CCNNNNNNNGG 1 cut(s) 114
Bse118I RCCGGY 1 cut(s) 74
Bse21I CCTNAGG 1 cut(s) 262
Bse3DI GCAATG 1 cut(s) 494
BseAI TCCGGA 2 cut(s) 114, 737
BseGI GGATG 3 cut(s) 239, 673, 707
BseLI CCNNNNNNNGG 1 cut(s) 114
BseMI GCAATG 1 cut(s) 494
BseMII CTCAG 1 cut(s) 78
BseRI GAGGAG 1 cut(s) 467
BseSI GKGCMC 1 cut(s) 50
BseXI GCAGC 3 cut(s) 382, 475, 694
BshFI GGCC 2 cut(s) 48, 513
BshTI ACCGGT 1 cut(s) 74
BsiHKAI GWGCWC 1 cut(s) 138
BsiSI CCGG 4 cut(s) 75, 115, 141, 738
BslI CCNNNNNNNGG 1 cut(s) 114
BsmAI GTCTC 1 cut(s) 112
BsnI GGCC 2 cut(s) 48, 513
Bsp120I GGGCCC 1 cut(s) 46
Bsp1286I GDGCHC 2 cut(s) 50, 138
Bsp13I TCCGGA 2 cut(s) 114, 737
Bsp143I GATC 4 cut(s) 63, 321, 348, 793
BspANI GGCC 2 cut(s) 48, 513
BspCNI CTCAG 1 cut(s) 79
BspEI TCCGGA 2 cut(s) 114, 737
BspLI GGNNCC 1 cut(s) 48
BspMAI CTGCAG 2 cut(s) 400, 700
BspMI ACCTGC 1 cut(s) 389
BspPI GGATC 2 cut(s) 356, 788
BsrDI GCAATG 1 cut(s) 494
BsrFI RCCGGY 1 cut(s) 74
BssAI RCCGGY 1 cut(s) 74
BssMI GATC 4 cut(s) 63, 321, 348, 793
BssNAI GTATAC 1 cut(s) 404
Bst1107I GTATAC 1 cut(s) 404
Bst4CI ACNGT 2 cut(s) 190, 685
Bst6I CTCTTC 1 cut(s) 444
BstDEI CTNAG 4 cut(s) 87, 262, 280, 798
BstF5I GGATG 3 cut(s) 239, 673, 707
BstKTI GATC 4 cut(s) 66, 324, 351, 796
BstMAI GTCTC 1 cut(s) 112
BstMBI GATC 4 cut(s) 63, 321, 348, 793
BstMWI GCNNNNNNNGC 1 cut(s) 704
BstSFI CTRYAG 3 cut(s) 396, 564, 696
BstSLI GKGCMC 1 cut(s) 50
BstV1I GCAGC 3 cut(s) 382, 475, 694
BstX2I RGATCY 1 cut(s) 321
BstYI RGATCY 1 cut(s) 321
BstZ17I GTATAC 1 cut(s) 404
Bsu36I CCTNAGG 1 cut(s) 262
BsuI GTATCC 1 cut(s) 590
BsuRI GGCC 2 cut(s) 48, 513
BtrI CACGTC 1 cut(s) 719
BtsCI GGATG 3 cut(s) 239, 673, 707
BtsIMutI CAGTG 1 cut(s) 84
BveI ACCTGC 1 cut(s) 389
Cfr10I RCCGGY 1 cut(s) 74
Cfr13I GGNCC 3 cut(s) 46, 47, 512
CspAI ACCGGT 1 cut(s) 74
CspCI CAANNNNNGTGG 2 cut(s) 7, 42
CviAII CATG 1 cut(s) 183
CviJI RGCY 9 cut(s) 48, 136, 201, 395, 442, 488, 513, 707, 760
CviKI_1 RGCY 9 cut(s) 48, 136, 201, 395, 442, 488, 513, 707, 760
DdeI CTNAG 4 cut(s) 87, 262, 280, 798
DpnI GATC 4 cut(s) 65, 323, 350, 795
DpnII GATC 4 cut(s) 63, 321, 348, 793
Eam1104I CTCTTC 1 cut(s) 444
EarI CTCTTC 1 cut(s) 444
Ecl136II GAGCTC 1 cut(s) 136
Eco24I GRGCYC 2 cut(s) 50, 138
Eco53kI GAGCTC 1 cut(s) 136
Eco81I CCTNAGG 1 cut(s) 262
EcoICRI GAGCTC 1 cut(s) 136
EcoO109I RGGNCCY 3 cut(s) 46, 47, 512
EcoRI GAATTC 1 cut(s) 275
EcoT38I GRGCYC 2 cut(s) 50, 138
FaeI CATG 1 cut(s) 186
FaiI YATR 8 cut(s) 32, 184, 220, 294, 305, 404, 549, 578
FatI CATG 1 cut(s) 182
FblI GTMKAC 1 cut(s) 403
Fnu4HI GCNGC 3 cut(s) 396, 489, 708
FokI GGATG 3 cut(s) 246, 680, 714
FriOI GRGCYC 2 cut(s) 50, 138
Fsp4HI GCNGC 3 cut(s) 396, 489, 708
GluI GCNGC 3 cut(s) 396, 489, 708
HaeIII GGCC 2 cut(s) 48, 513
HapII CCGG 4 cut(s) 75, 115, 141, 738
Hin1II CATG 1 cut(s) 186
HinfI GANTC 2 cut(s) 99, 167
HpaII CCGG 4 cut(s) 75, 115, 141, 738
HphI GGTGA 1 cut(s) 395
Hpy166II GTNNAC 3 cut(s) 404, 522, 787
Hpy188I TCNGA 2 cut(s) 368, 625
Hpy188III TCNNGA 6 cut(s) 61, 115, 226, 359, 613, 738
Hpy8I GTNNAC 3 cut(s) 404, 522, 787
HpyAV CCTTC 3 cut(s) 203, 503, 658
HpyCH4III ACNGT 2 cut(s) 190, 685
HpyCH4IV ACGT 3 cut(s) 53, 353, 718
HpyCH4V TGCA 5 cut(s) 307, 398, 499, 698, 750
HpyF10VI GCNNNNNNNGC 1 cut(s) 704
HpyF3I CTNAG 4 cut(s) 87, 262, 280, 798
HpySE526I ACGT 3 cut(s) 53, 353, 718
Hsp92II CATG 1 cut(s) 186
Kpn2I TCCGGA 2 cut(s) 114, 737
Kzo9I GATC 4 cut(s) 63, 321, 348, 793
Lsp1109I GCAGC 3 cut(s) 382, 475, 694
LweI GCATC 1 cut(s) 658
MaeII ACGT 3 cut(s) 53, 353, 718
MaeIII GTNAC 2 cut(s) 266, 719
MalI GATC 4 cut(s) 65, 323, 350, 795
MboI GATC 4 cut(s) 63, 321, 348, 793
MboII GAAGA 6 cut(s) 49, 162, 461, 470, 491, 494
MflI RGATCY 1 cut(s) 321
MhlI GDGCHC 2 cut(s) 50, 138
MluCI AATT 8 cut(s) 110, 157, 275, 308, 388, 502, 550, 619
MmeI TCCRAC 2 cut(s) 450, 514
MnlI CCTC 8 cut(s) 28, 82, 339, 374, 445, 448, 600, 619
MroI TCCGGA 2 cut(s) 114, 737
MseI TTAA 3 cut(s) 161, 444, 687
MspA1I CMGCKG 1 cut(s) 395
MspI CCGG 4 cut(s) 75, 115, 141, 738
MwoI GCNNNNNNNGC 1 cut(s) 704
NdeII GATC 4 cut(s) 63, 321, 348, 793
NlaIII CATG 1 cut(s) 186
NlaIV GGNNCC 1 cut(s) 48
NmuCI GTSAC 1 cut(s) 719
PfeI GAWTC 2 cut(s) 99, 167
PinAI ACCGGT 1 cut(s) 74
PkrI GCNGC 3 cut(s) 397, 490, 709
PsiI TTATAA 1 cut(s) 549
Psp124BI GAGCTC 1 cut(s) 138
PspN4I GGNNCC 1 cut(s) 48
PspOMI GGGCCC 1 cut(s) 46
PspPI GGNCC 3 cut(s) 46, 47, 512
PstI CTGCAG 2 cut(s) 400, 700
PsuI RGATCY 1 cut(s) 321
PvuII CAGCTG 1 cut(s) 395
SacI GAGCTC 1 cut(s) 138
SaqAI TTAA 3 cut(s) 161, 444, 687
SatI GCNGC 3 cut(s) 396, 489, 708
Sau3AI GATC 4 cut(s) 63, 321, 348, 793
Sau96I GGNCC 3 cut(s) 46, 47, 512
SduI GDGCHC 2 cut(s) 50, 138
SfaNI GCATC 1 cut(s) 658
SfcI CTRYAG 3 cut(s) 396, 564, 696
Sse9I AATT 8 cut(s) 110, 157, 275, 308, 388, 502, 550, 619
SstI GAGCTC 1 cut(s) 138
TaaI ACNGT 2 cut(s) 190, 685
TaiI ACGT 3 cut(s) 56, 356, 721
TasI AATT 8 cut(s) 110, 157, 275, 308, 388, 502, 550, 619
TfiI GAWTC 2 cut(s) 99, 167
Tru1I TTAA 3 cut(s) 161, 444, 687
Tru9I TTAA 3 cut(s) 161, 444, 687
TscAI CASTG 1 cut(s) 91
TseFI GTSAC 1 cut(s) 719
TseI GCWGC 3 cut(s) 395, 488, 707
Tsp45I GTSAC 1 cut(s) 719
TspDTI ATGAA 2 cut(s) 171, 593
TspRI CASTG 1 cut(s) 91
XapI RAATTY 5 cut(s) 110, 157, 275, 388, 619
XmiI GTMKAC 1 cut(s) 403
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.