B3 domain-containing protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 646 |
| AccB1I | GGYRCC | 1 cut(s) | 1976 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1735 |
| AciI | CCGC | 2 cut(s) | 147, 1304 |
| AclI | AACGTT | 1 cut(s) | 1189 |
| AclWI | GGATC | 4 cut(s) | 447, 697, 1746, 1818 |
| AcoI | YGGCCR | 1 cut(s) | 1711 |
| AcsI | RAATTY | 8 cut(s) | 335, 499, 748, 777, 1140, 1345, 1511, 2064 |
| AcuI | CTGAAG | 2 cut(s) | 1581, 1811 |
| AcyI | GRCGYC | 1 cut(s) | 1847 |
| AfaI | GTAC | 3 cut(s) | 1637, 1806, 1892 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 221, 222, 1735 |
| AflIII | ACRYGT | 1 cut(s) | 2201 |
| AhlI | ACTAGT | 1 cut(s) | 506 |
| AluBI | AGCT | 9 cut(s) | 93, 110, 378, 494, 859, 1390, 1559, 1565, 1735 |
| AluI | AGCT | 9 cut(s) | 93, 110, 378, 494, 859, 1390, 1559, 1565, 1735 |
| Alw21I | GWGCWC | 3 cut(s) | 840, 1532, 2036 |
| Alw26I | GTCTC | 1 cut(s) | 1891 |
| Alw44I | GTGCAC | 1 cut(s) | 1528 |
| AlwI | GGATC | 4 cut(s) | 447, 697, 1746, 1818 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1735 |
| AoxI | GGCC | 2 cut(s) | 1104, 1711 |
| ApaLI | GTGCAC | 1 cut(s) | 1528 |
| ApeKI | GCWGC | 3 cut(s) | 90, 1720, 1732 |
| ApoI | RAATTY | 8 cut(s) | 335, 499, 748, 777, 1140, 1345, 1511, 2064 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 642, 674, 1981, 2013 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 63 |
| AspA2I | CCTAGG | 1 cut(s) | 1669 |
| AspS9I | GGNCC | 3 cut(s) | 539, 1907, 2126 |
| AsuC2I | CCSGG | 1 cut(s) | 1844 |
| AsuHPI | GGTGA | 4 cut(s) | 190, 781, 989, 1286 |
| AvaII | GGWCC | 3 cut(s) | 539, 1907, 2126 |
| AvrII | CCTAGG | 1 cut(s) | 1669 |
| BaeGI | GKGCMC | 2 cut(s) | 1532, 1981 |
| BalI | TGGCCA | 1 cut(s) | 1713 |
| BanI | GGYRCC | 1 cut(s) | 1976 |
| BanII | GRGCYC | 1 cut(s) | 228 |
| BarI | GAAGNNNNNNTAC | 4 cut(s) | 610, 642, 1357, 1389 |
| BauI | CACGAG | 1 cut(s) | 1075 |
| BbsI | GAAGAC | 2 cut(s) | 114, 652 |
| Bbv12I | GWGCWC | 3 cut(s) | 840, 1532, 2036 |
| BbvI | GCAGC | 3 cut(s) | 102, 1732, 1744 |
| BccI | CCATC | 9 cut(s) | 182, 412, 424, 427, 556, 855, 903, 1219, 1924 |
| BciVI | GTATCC | 1 cut(s) | 1212 |
| BcnI | CCSGG | 1 cut(s) | 1844 |
| BcoDI | GTCTC | 1 cut(s) | 1891 |
| BcuI | ACTAGT | 1 cut(s) | 506 |
| BfaI | CTAG | 7 cut(s) | 323, 368, 507, 1118, 1611, 1670, 2174 |
| BfmI | CTRYAG | 3 cut(s) | 653, 1603, 1701 |
| BfuAI | ACCTGC | 1 cut(s) | 646 |
| BfuI | GTATCC | 1 cut(s) | 1212 |
| BisI | GCNGC | 3 cut(s) | 91, 1721, 1733 |
| BlnI | CCTAGG | 1 cut(s) | 1669 |
| BlsI | GCNGC | 3 cut(s) | 92, 1722, 1734 |
| Bme1390I | CCNGG | 1 cut(s) | 1844 |
| Bme18I | GGWCC | 3 cut(s) | 539, 1907, 2126 |
| BmgT120I | GGNCC | 3 cut(s) | 539, 1907, 2126 |
| BmiI | GGNNCC | 4 cut(s) | 961, 1978, 2128, 2183 |
| BmrFI | CCNGG | 1 cut(s) | 1844 |
| BmsI | GCATC | 4 cut(s) | 1732, 2008, 2032, 2068 |
| BpiI | GAAGAC | 2 cut(s) | 114, 652 |
| BpmI | CTGGAG | 2 cut(s) | 1527, 1712 |
| BpuEI | CTTGAG | 1 cut(s) | 1551 |
| BpuMI | CCSGG | 1 cut(s) | 1844 |
| BsaAI | YACGTR | 1 cut(s) | 953 |
| BsaHI | GRCGYC | 1 cut(s) | 1847 |
| BsaJI | CCNNGG | 4 cut(s) | 1669, 2052, 2069, 2130 |
| BsaWI | WCCGGW | 1 cut(s) | 536 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1742, 1772 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 221, 222, 1735 |
| Bse1I | ACTGG | 4 cut(s) | 420, 1263, 1438, 1812 |
| Bse3DI | GCAATG | 1 cut(s) | 1218 |
| BseDI | CCNNGG | 4 cut(s) | 1669, 2052, 2069, 2130 |
| BseGI | GGATG | 3 cut(s) | 416, 438, 548 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 221, 222, 1735 |
| BseMI | GCAATG | 1 cut(s) | 1218 |
| BseMII | CTCAG | 1 cut(s) | 143 |
| BseNI | ACTGG | 4 cut(s) | 420, 1263, 1438, 1812 |
| BseRI | GAGGAG | 2 cut(s) | 117, 2233 |
| BseSI | GKGCMC | 2 cut(s) | 1532, 1981 |
| BseXI | GCAGC | 3 cut(s) | 102, 1732, 1744 |
| BseYI | CCCAGC | 1 cut(s) | 1453 |
| BsgI | GTGCAG | 1 cut(s) | 486 |
| BshFI | GGCC | 2 cut(s) | 1106, 1713 |
| BshNI | GGYRCC | 1 cut(s) | 1976 |
| BsiHKAI | GWGCWC | 3 cut(s) | 840, 1532, 2036 |
| BsiSI | CCGG | 3 cut(s) | 537, 1761, 1844 |
| BslFI | GGGAC | 2 cut(s) | 1471, 2112 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 221, 222, 1735 |
| BsmAI | GTCTC | 1 cut(s) | 1891 |
| BsmFI | GGGAC | 2 cut(s) | 1471, 2112 |
| BsmI | GAATGC | 1 cut(s) | 11 |
| BsnI | GGCC | 2 cut(s) | 1106, 1713 |
| Bsp1286I | GDGCHC | 5 cut(s) | 228, 840, 1532, 1981, 2036 |
| Bsp143I | GATC | 7 cut(s) | 325, 355, 439, 689, 1404, 1738, 1810 |
| Bsp19I | CCATGG | 1 cut(s) | 2069 |
| BspACI | CCGC | 2 cut(s) | 147, 1304 |
| BspANI | GGCC | 2 cut(s) | 1106, 1713 |
| BspCNI | CTCAG | 1 cut(s) | 142 |
| BspLI | GGNNCC | 4 cut(s) | 961, 1978, 2128, 2183 |
| BspMAI | CTGCAG | 1 cut(s) | 657 |
| BspMI | ACCTGC | 1 cut(s) | 646 |
| BspPI | GGATC | 4 cut(s) | 447, 697, 1746, 1818 |
| BspT107I | GGYRCC | 1 cut(s) | 1976 |
| BsrDI | GCAATG | 1 cut(s) | 1218 |
| BsrI | ACTGG | 4 cut(s) | 420, 1263, 1438, 1812 |
| BssECI | CCNNGG | 4 cut(s) | 1669, 2052, 2069, 2130 |
| BssMI | GATC | 7 cut(s) | 325, 355, 439, 689, 1404, 1738, 1810 |
| BssNI | GRCGYC | 1 cut(s) | 1847 |
| BssSI | CACGAG | 1 cut(s) | 1075 |
| BssT1I | CCWWGG | 4 cut(s) | 1669, 2052, 2069, 2130 |
| Bst2BI | CACGAG | 1 cut(s) | 1075 |
| Bst4CI | ACNGT | 3 cut(s) | 1292, 1607, 1635 |
| Bst6I | CTCTTC | 3 cut(s) | 46, 723, 1467 |
| BstACI | GRCGYC | 1 cut(s) | 1847 |
| BstBAI | YACGTR | 1 cut(s) | 953 |
| BstC8I | GCNNGC | 3 cut(s) | 73, 1620, 2212 |
| BstDEI | CTNAG | 7 cut(s) | 106, 129, 1007, 1016, 1358, 1685, 1742 |
| BstDSI | CCRYGG | 1 cut(s) | 2069 |
| BstEII | GGTNACC | 1 cut(s) | 196 |
| BstF5I | GGATG | 3 cut(s) | 416, 438, 548 |
| BstKTI | GATC | 7 cut(s) | 328, 358, 442, 692, 1407, 1741, 1813 |
| BstMAI | GTCTC | 1 cut(s) | 1891 |
| BstMBI | GATC | 7 cut(s) | 325, 355, 439, 689, 1404, 1738, 1810 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 1387, 1729 |
| BstNSI | RCATGY | 2 cut(s) | 1489, 1622 |
| BstPI | GGTNACC | 1 cut(s) | 196 |
| BstSCI | CCNGG | 1 cut(s) | 1842 |
| BstSFI | CTRYAG | 3 cut(s) | 653, 1603, 1701 |
| BstSLI | GKGCMC | 2 cut(s) | 1532, 1981 |
| BstSNI | TACGTA | 1 cut(s) | 953 |
| BstV1I | GCAGC | 3 cut(s) | 102, 1732, 1744 |
| BstV2I | GAAGAC | 2 cut(s) | 114, 652 |
| BstX2I | RGATCY | 3 cut(s) | 439, 1738, 1810 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 830, 1505 |
| BstYI | RGATCY | 3 cut(s) | 439, 1738, 1810 |
| BsuI | GTATCC | 1 cut(s) | 1212 |
| BsuRI | GGCC | 2 cut(s) | 1106, 1713 |
| BtgI | CCRYGG | 1 cut(s) | 2069 |
| BtsCI | GGATG | 3 cut(s) | 416, 438, 548 |
| BtsI | GCAGTG | 1 cut(s) | 771 |
| BtsIMutI | CAGTG | 3 cut(s) | 771, 1297, 1431 |
| BveI | ACCTGC | 1 cut(s) | 646 |
| Cac8I | GCNNGC | 3 cut(s) | 73, 1620, 2212 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1735 |
| Cfr13I | GGNCC | 3 cut(s) | 539, 1907, 2126 |
| CseI | GACGC | 1 cut(s) | 1836 |
| Csp6I | GTAC | 3 cut(s) | 1636, 1805, 1891 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1083, 1118 |
| CviQI | GTAC | 3 cut(s) | 1636, 1805, 1891 |
| DdeI | CTNAG | 7 cut(s) | 106, 129, 1007, 1016, 1358, 1685, 1742 |
| DpnI | GATC | 7 cut(s) | 327, 357, 441, 691, 1406, 1740, 1812 |
| DpnII | GATC | 7 cut(s) | 325, 355, 439, 689, 1404, 1738, 1810 |
| EaeI | YGGCCR | 1 cut(s) | 1711 |
| Eam1104I | CTCTTC | 3 cut(s) | 46, 723, 1467 |
| EarI | CTCTTC | 3 cut(s) | 46, 723, 1467 |
| EciI | GGCGGA | 1 cut(s) | 1319 |
| Eco105I | TACGTA | 1 cut(s) | 953 |
| Eco130I | CCWWGG | 4 cut(s) | 1669, 2052, 2069, 2130 |
| Eco24I | GRGCYC | 1 cut(s) | 228 |
| Eco32I | GATATC | 2 cut(s) | 706, 982 |
| Eco47I | GGWCC | 3 cut(s) | 539, 1907, 2126 |
| Eco57I | CTGAAG | 2 cut(s) | 1581, 1811 |
| Eco91I | GGTNACC | 1 cut(s) | 196 |
| EcoO65I | GGTNACC | 1 cut(s) | 196 |
| EcoRI | GAATTC | 3 cut(s) | 335, 748, 1345 |
| EcoRV | GATATC | 2 cut(s) | 706, 982 |
| EcoT14I | CCWWGG | 4 cut(s) | 1669, 2052, 2069, 2130 |
| EcoT22I | ATGCAT | 2 cut(s) | 13, 2161 |
| EcoT38I | GRGCYC | 1 cut(s) | 228 |
| ErhI | CCWWGG | 4 cut(s) | 1669, 2052, 2069, 2130 |
| FaqI | GGGAC | 2 cut(s) | 1471, 2112 |
| Fnu4HI | GCNGC | 3 cut(s) | 91, 1721, 1733 |
| FokI | GGATG | 3 cut(s) | 403, 445, 535 |
| FriOI | GRGCYC | 1 cut(s) | 228 |
| Fsp4HI | GCNGC | 3 cut(s) | 91, 1721, 1733 |
| FspBI | CTAG | 7 cut(s) | 323, 368, 507, 1118, 1611, 1670, 2174 |
| GluI | GCNGC | 3 cut(s) | 91, 1721, 1733 |
| GsaI | CCCAGC | 1 cut(s) | 1457 |
| GsuI | CTGGAG | 2 cut(s) | 1527, 1712 |
| HaeIII | GGCC | 2 cut(s) | 1106, 1713 |
| HapII | CCGG | 3 cut(s) | 537, 1761, 1844 |
| HgaI | GACGC | 1 cut(s) | 1836 |
| Hin1I | GRCGYC | 1 cut(s) | 1847 |
| HindIII | AAGCTT | 1 cut(s) | 492 |
| HinfI | GANTC | 6 cut(s) | 292, 1428, 1459, 1878, 1963, 2118 |
| HpaII | CCGG | 3 cut(s) | 537, 1761, 1844 |
| HphI | GGTGA | 4 cut(s) | 190, 781, 989, 1286 |
| Hpy166II | GTNNAC | 3 cut(s) | 458, 1022, 1530 |
| Hpy188I | TCNGA | 6 cut(s) | 432, 747, 924, 974, 1003, 2001 |
| Hpy8I | GTNNAC | 3 cut(s) | 458, 1022, 1530 |
| HpyAV | CCTTC | 8 cut(s) | 161, 395, 576, 610, 896, 1174, 1383, 2139 |
| HpyCH4III | ACNGT | 3 cut(s) | 1292, 1607, 1635 |
| HpyCH4IV | ACGT | 3 cut(s) | 952, 1189, 2201 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 1387, 1729 |
| HpyF3I | CTNAG | 7 cut(s) | 106, 129, 1007, 1016, 1358, 1685, 1742 |
| HpySE526I | ACGT | 3 cut(s) | 952, 1189, 2201 |
| Hsp92I | GRCGYC | 1 cut(s) | 1847 |
| Kzo9I | GATC | 7 cut(s) | 325, 355, 439, 689, 1404, 1738, 1810 |
| LmnI | GCTCC | 4 cut(s) | 761, 843, 959, 2056 |
| Lsp1109I | GCAGC | 3 cut(s) | 102, 1732, 1744 |
| LweI | GCATC | 4 cut(s) | 1732, 2008, 2032, 2068 |
| MaeI | CTAG | 7 cut(s) | 323, 368, 507, 1118, 1611, 1670, 2174 |
| MaeII | ACGT | 3 cut(s) | 952, 1189, 2201 |
| MaeIII | GTNAC | 9 cut(s) | 196, 210, 399, 521, 579, 671, 1352, 1398, 1673 |
| MalI | GATC | 7 cut(s) | 327, 357, 441, 691, 1406, 1740, 1812 |
| MboI | GATC | 7 cut(s) | 325, 355, 439, 689, 1404, 1738, 1810 |
| MfeI | CAATTG | 3 cut(s) | 160, 824, 1678 |
| MflI | RGATCY | 3 cut(s) | 439, 1738, 1810 |
| MhlI | GDGCHC | 5 cut(s) | 228, 840, 1532, 1981, 2036 |
| MlsI | TGGCCA | 1 cut(s) | 1713 |
| MluNI | TGGCCA | 1 cut(s) | 1713 |
| MlyI | GAGTC | 2 cut(s) | 1453, 2127 |
| MmeI | TCCRAC | 3 cut(s) | 471, 511, 807 |
| Mox20I | TGGCCA | 1 cut(s) | 1713 |
| Mph1103I | ATGCAT | 2 cut(s) | 13, 2161 |
| MroXI | GAANNNNTTC | 1 cut(s) | 63 |
| MscI | TGGCCA | 1 cut(s) | 1713 |
| MseI | TTAA | 4 cut(s) | 711, 1311, 1481, 1863 |
| Msp20I | TGGCCA | 1 cut(s) | 1713 |
| MspA1I | CMGCKG | 2 cut(s) | 149, 1735 |
| MspI | CCGG | 3 cut(s) | 537, 1761, 1844 |
| MspR9I | CCNGG | 1 cut(s) | 1844 |
| MunI | CAATTG | 3 cut(s) | 160, 824, 1678 |
| Mva1269I | GAATGC | 1 cut(s) | 11 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 1387, 1729 |
| NciI | CCSGG | 1 cut(s) | 1844 |
| NcoI | CCATGG | 1 cut(s) | 2069 |
| NdeII | GATC | 7 cut(s) | 325, 355, 439, 689, 1404, 1738, 1810 |
| NlaIV | GGNNCC | 4 cut(s) | 961, 1978, 2128, 2183 |
| NmeAIII | GCCGAG | 1 cut(s) | 1086 |
| NmuCI | GTSAC | 2 cut(s) | 196, 1673 |
| NsiI | ATGCAT | 2 cut(s) | 13, 2161 |
| NspI | RCATGY | 2 cut(s) | 1489, 1622 |
| PaeI | GCATGC | 1 cut(s) | 1622 |
| PctI | GAATGC | 1 cut(s) | 11 |
| PdmI | GAANNNNTTC | 1 cut(s) | 63 |
| PfeI | GAWTC | 4 cut(s) | 292, 1428, 1878, 1963 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1735 |
| PkrI | GCNGC | 3 cut(s) | 92, 1722, 1734 |
| PleI | GAGTC | 2 cut(s) | 1453, 2126 |
| PpsI | GAGTC | 2 cut(s) | 1453, 2126 |
| Ppu21I | YACGTR | 1 cut(s) | 953 |
| Psp1406I | AACGTT | 1 cut(s) | 1189 |
| PspEI | GGTNACC | 1 cut(s) | 196 |
| PspFI | CCCAGC | 1 cut(s) | 1453 |
| PspN4I | GGNNCC | 4 cut(s) | 961, 1978, 2128, 2183 |
| PspPI | GGNCC | 3 cut(s) | 539, 1907, 2126 |
| PstI | CTGCAG | 1 cut(s) | 657 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1735 |
| PsuI | RGATCY | 3 cut(s) | 439, 1738, 1810 |
| PvuII | CAGCTG | 1 cut(s) | 1735 |
| RsaI | GTAC | 3 cut(s) | 1637, 1806, 1892 |
| RsaNI | GTAC | 3 cut(s) | 1636, 1805, 1891 |
| SaqAI | TTAA | 4 cut(s) | 711, 1311, 1481, 1863 |
| SatI | GCNGC | 3 cut(s) | 91, 1721, 1733 |
| Sau3AI | GATC | 7 cut(s) | 325, 355, 439, 689, 1404, 1738, 1810 |
| Sau96I | GGNCC | 3 cut(s) | 539, 1907, 2126 |
| SchI | GAGTC | 2 cut(s) | 1453, 2127 |
| ScrFI | CCNGG | 1 cut(s) | 1844 |
| SduI | GDGCHC | 5 cut(s) | 228, 840, 1532, 1981, 2036 |
| SfaNI | GCATC | 4 cut(s) | 1732, 2008, 2032, 2068 |
| SfcI | CTRYAG | 3 cut(s) | 653, 1603, 1701 |
| SinI | GGWCC | 3 cut(s) | 539, 1907, 2126 |
| SmlI | CTYRAG | 1 cut(s) | 1566 |
| SmoI | CTYRAG | 1 cut(s) | 1566 |
| SnaBI | TACGTA | 1 cut(s) | 953 |
| SpeI | ACTAGT | 1 cut(s) | 506 |
| SphI | GCATGC | 1 cut(s) | 1622 |
| SsiI | CCGC | 2 cut(s) | 147, 1304 |
| SspMI | CTAG | 7 cut(s) | 323, 368, 507, 1118, 1611, 1670, 2174 |
| StyD4I | CCNGG | 1 cut(s) | 1842 |
| StyI | CCWWGG | 4 cut(s) | 1669, 2052, 2069, 2130 |
| TaaI | ACNGT | 3 cut(s) | 1292, 1607, 1635 |
| TaiI | ACGT | 3 cut(s) | 955, 1192, 2204 |
| TaqI | TCGA | 5 cut(s) | 184, 360, 1026, 1099, 1385 |
| TatI | WGTACW | 1 cut(s) | 1635 |
| TfiI | GAWTC | 4 cut(s) | 292, 1428, 1878, 1963 |
| Tru1I | TTAA | 4 cut(s) | 711, 1311, 1481, 1863 |
| Tru9I | TTAA | 4 cut(s) | 711, 1311, 1481, 1863 |
| TscAI | CASTG | 3 cut(s) | 771, 1297, 1438 |
| TseFI | GTSAC | 2 cut(s) | 196, 1673 |
| TseI | GCWGC | 3 cut(s) | 90, 1720, 1732 |
| Tsp45I | GTSAC | 2 cut(s) | 196, 1673 |
| TspGWI | ACGGA | 1 cut(s) | 653 |
| TspRI | CASTG | 3 cut(s) | 771, 1297, 1438 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1735 |
| VneI | GTGCAC | 1 cut(s) | 1528 |
| VpaK11BI | GGWCC | 3 cut(s) | 539, 1907, 2126 |
| XapI | RAATTY | 8 cut(s) | 335, 499, 748, 777, 1140, 1345, 1511, 2064 |
| XbaI | TCTAGA | 2 cut(s) | 322, 1117 |
| XceI | RCATGY | 2 cut(s) | 1489, 1622 |
| XmaJI | CCTAGG | 1 cut(s) | 1669 |
| XmnI | GAANNNNTTC | 1 cut(s) | 63 |
| XspI | CTAG | 7 cut(s) | 323, 368, 507, 1118, 1611, 1670, 2174 |
| Zsp2I | ATGCAT | 2 cut(s) | 13, 2161 |