RLG00000014545

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0000235
Physical Location & Seq
Forward (+)
54559076 .. 54560305
1230 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014545

Sequence Viewer

Length: 741 bp
ATGGCGAGGAGAATAGCCAAGAGTGGTGGTACAAGGAAGAGACCTTCTTTCTTCAAGGTCATTCTTCCCGGTTACAACACTGAACAGTTGAGGATTCCACCGCATTTCCTAGAGCATGGTAGAAATGACCAACCTGAAAGGGCAACTTTGACATGGAACATGTCTTCAGAGCGTTCGTGGACTGTCAAAGTGTGTAGAACAAAAGAAGGTGTGTATTTCAAGGATGGGTGGCGGGATTTTTTAAATGATAACTCAGTGAAGGAAGGTGACTTCCTAGTGTTCACCTATGAAGGGGAGATGCATTTCATCATACGTGATTACGAAAATAATTGTTGCAAGAGAGGCGATTTTCCTACTAATAATGAACCACACGAAGAATCCGCCCCTGCTAAGAGTACTAAAAGGAATCCTGGTAGTCCAAAGAAAGATTATTCAGCTGAATCTTTCCGGTCCAAGTTTCCTCATTACACAAGATCTATCAAGAAAAAATATTGTGTGTACTCAATTCCCACAGCCTTCTATAGGAAGCATCTTTCTGCAGGCAACTATGAAGTTGCTTTCCTGAAGATTTCAGGGGGAACCAAATGGTACAAAGTGAAGCTTGTCCCTTCCCTTCAAACAGCCTTCATGTCTAAAGGGTGGTCTGTGTTTGCGCATGATAACCAAATCGTGGTTGGAGATGTTTGCTCTTTCGAGCTCATGAAAGAGAACAAAATGGTGGTTCACATTCTCCGGAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0007275 GO:0008150 GO:0009553 GO:0009566 GO:0009567 GO:0019953 GO:0022414 GO:0032501 GO:0032502 GO:0044703 GO:0048229 GO:0048856 GO:0051704
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

247

Amino Acids

28.6

Weight (kDa)

9.64

Isoelectric Point (pI)

45.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 17 - 102 1.6e-18 B3 DNA binding domain
B3 PF02362 162 - 246 2.4e-12 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000235)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g22700 FvH4_2g06920 FvH4_2g06920 FvH4_2g06960 FvH4_2g06960 FvH4_2g06960 FvH4_2g06960 FvH4_2g06970 FvH4_2g06970 FvH4_2g07230 FvH4_2g07231 FvH4_2g07231 FvH4_2g07240 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_4g19990 FvH4_4g19990 FvH4_4g19990 FvH4_4g20070
malus_domestica MD05G1072700.v1.1
prunus_persica Prupe.8G113100_v2.0.a1
pyrus_communis pycom05g06500 pycom10g06950
rosa_chinensis RchiOBHm_Chr4g0425711 RchiOBHm_Chr6g0259241 RchiOBHm_Chr6g0259251 RchiOBHm_Chr6g0259321 RchiOBHm_Chr6g0259331 RchiOBHm_Chr6g0259781 RchiOBHm_Chr6g0259791 RchiOBHm_Chr6g0259811 RchiOBHm_Chr6g0259881 RchiOBHm_Chr6g0259931
rosa_laevigata RLG00000007345 RLG00000014500 RLG00000014501 RLG00000014505 RLG00000014506 RLG00000014507 RLG00000014539 RLG00000014540 RLG00000014545 RLG00000014546
rosa_multiflora Rmu_co8137334.1_g000001 Rmu_co8454279.1_g000001 Rmu_co8476373.1_g000001 Rmu_sc0001078.1_g000001 Rmu_sc0003958.1_g000011 Rmu_sc0009049.1_g000001 Rmu_sc0009049.1_g000009 Rmu_sc0010546.1_g000001 Rmu_sc0011974.1_g000002 Rmu_sc0011974.1_g000003 Rmu_sc0033846.1_g000001 Rmu_sc0036999.1_g000001 Rmu_ssc0000052.1_g000015
rosa_roxburghii Rroxscaffold_5G00367690 Rroxscaffold_7G00206530 Rroxscaffold_7G00206540 Rroxscaffold_7G00206560 Rroxscaffold_7G00206570 Rroxscaffold_7G00207020 Rroxscaffold_7G00207030 Rroxscaffold_7G00207150 Rroxscaffold_7G00207160 Rroxscaffold_7G00207170 Rroxscaffold_7G00207270
rosa_rugosa Rorug04G0205300 Rorug05G0586300 Rorug05G0586400 Rorug05G0586500 Rorug05G0586600 Rorug05G0586700 Rorug05G0587300 Rorug05G0587400 Rorug05G0587500 Rorug05G0587600 Rorug05G0590500 Rorug05G0590600 Rorug05G0591200 Rorug05G0591300 Rorug05G0591400 Rorug05G0591500
rosa_samantha Rh4AG262200 Rh4BG268500 Rh4CG279700 Rh4DG264100 Rh6AG103100 Rh6AG103600 Rh6AG107100 Rh6AG107200 Rh6AG107700 Rh6BG095400 Rh6BG095500 Rh6BG095900 Rh6BG096000 Rh6BG096800 Rh6BG102600 Rh6BG102700 Rh6BG102800 Rh6BG103800 Rh6CG092300 Rh6CG092400 Rh6CG092800 Rh6CG092900 Rh6CG096200 Rh6CG096300 Rh6CG096800 Rh6CG097100 Rh6DG086300 Rh6DG086400 Rh6DG086700 Rh6DG086800 Rh6DG090000 Rh6DG090100 Rh6DG090500 Rh6DG090800
rosa_wichuraiana Rw4G022670 Rw6G008900 Rw6G008940 Rw6G009250 Rw6G009280 Rw6G009330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 654
AccB7I CCANNNNNTGG 1 cut(s) 670
AccIII TCCGGA 1 cut(s) 732
AciI CCGC 3 cut(s) 101, 232, 381
AcuI CTGAAG 2 cut(s) 150, 584
AfaI GTAC 4 cut(s) 31, 397, 500, 590
AfiI CCNNNNNNNGG 3 cut(s) 291, 522, 670
AflIII ACRYGT 1 cut(s) 159
AgsI TTSAA 3 cut(s) 55, 220, 617
AjnI CCWGG 1 cut(s) 409
AluBI AGCT 3 cut(s) 437, 601, 697
AluI AGCT 3 cut(s) 437, 601, 697
Alw21I GWGCWC 1 cut(s) 699
Alw26I GTCTC 1 cut(s) 34
Aor13HI TCCGGA 1 cut(s) 732
AspLEI GCGC 1 cut(s) 655
AspS9I GGNCC 1 cut(s) 450
AsuC2I CCSGG 1 cut(s) 69
AsuHPI GGTGA 2 cut(s) 274, 278
AvaII GGWCC 1 cut(s) 450
BanII GRGCYC 1 cut(s) 699
BbsI GAAGAC 1 cut(s) 156
Bbv12I GWGCWC 1 cut(s) 699
BccI CCATC 1 cut(s) 218
BciT130I CCWGG 1 cut(s) 411
BcnI CCSGG 1 cut(s) 69
BcoDI GTCTC 1 cut(s) 34
BfaI CTAG 2 cut(s) 110, 275
BfmI CTRYAG 2 cut(s) 520, 537
BglII AGATCT 1 cut(s) 473
BmcAI AGTACT 1 cut(s) 397
Bme1390I CCNGG 2 cut(s) 69, 411
Bme18I GGWCC 1 cut(s) 450
BmgT120I GGNCC 1 cut(s) 450
BmiI GGNNCC 1 cut(s) 580
BmrFI CCNGG 2 cut(s) 69, 411
BmsI GCATC 2 cut(s) 288, 538
BpiI GAAGAC 1 cut(s) 156
BpuMI CCSGG 1 cut(s) 69
BsaAI YACGTR 1 cut(s) 314
BsaI GGTCTC 1 cut(s) 34
BsaWI WCCGGW 2 cut(s) 447, 732
Bsc4I CCNNNNNNNGG 3 cut(s) 291, 522, 670
BseAI TCCGGA 1 cut(s) 732
BseBI CCWGG 1 cut(s) 411
BseGI GGATG 1 cut(s) 229
BseLI CCNNNNNNNGG 3 cut(s) 291, 522, 670
BseMII CTCAG 1 cut(s) 267
BseRI GAGGAG 1 cut(s) 22
BsiHKAI GWGCWC 1 cut(s) 699
BsiSI CCGG 3 cut(s) 69, 448, 733
BslFI GGGAC 1 cut(s) 590
BslI CCNNNNNNNGG 3 cut(s) 291, 522, 670
BsmAI GTCTC 1 cut(s) 34
BsmFI GGGAC 1 cut(s) 590
Bso31I GGTCTC 1 cut(s) 34
Bsp1286I GDGCHC 1 cut(s) 699
Bsp13I TCCGGA 1 cut(s) 732
Bsp143I GATC 1 cut(s) 473
BspACI CCGC 3 cut(s) 101, 232, 381
BspCNI CTCAG 1 cut(s) 266
BspEI TCCGGA 1 cut(s) 732
BspHI TCATGA 1 cut(s) 699
BspLI GGNNCC 1 cut(s) 580
BspMAI CTGCAG 1 cut(s) 541
BspTNI GGTCTC 1 cut(s) 34
BssMI GATC 1 cut(s) 473
Bst2UI CCWGG 1 cut(s) 411
Bst4CI ACNGT 2 cut(s) 87, 184
Bst6I CTCTTC 1 cut(s) 32
BstBAI YACGTR 1 cut(s) 314
BstC8I GCNNGC 1 cut(s) 541
BstDEI CTNAG 2 cut(s) 253, 390
BstENI CCTNNNNNAGG 1 cut(s) 520
BstF5I GGATG 1 cut(s) 229
BstHHI GCGC 1 cut(s) 655
BstKTI GATC 1 cut(s) 476
BstMAI GTCTC 1 cut(s) 34
BstMBI GATC 1 cut(s) 473
BstMWI GCNNNNNNNGC 1 cut(s) 342
BstNI CCWGG 1 cut(s) 411
BstNSI RCATGY 1 cut(s) 163
BstSCI CCNGG 2 cut(s) 67, 409
BstSFI CTRYAG 2 cut(s) 520, 537
BstV2I GAAGAC 1 cut(s) 156
BstX2I RGATCY 1 cut(s) 473
BstYI RGATCY 1 cut(s) 473
BtsCI GGATG 1 cut(s) 229
BtsIMutI CAGTG 2 cut(s) 78, 261
Cac8I GCNNGC 1 cut(s) 541
CciI TCATGA 1 cut(s) 699
CfoI GCGC 1 cut(s) 655
Cfr13I GGNCC 1 cut(s) 450
Csp6I GTAC 4 cut(s) 30, 396, 499, 589
CspCI CAANNNNNGTGG 2 cut(s) 7, 42
CviAII CATG 6 cut(s) 116, 153, 160, 628, 656, 700
CviJI RGCY 6 cut(s) 17, 437, 515, 601, 623, 697
CviKI_1 RGCY 6 cut(s) 17, 437, 515, 601, 623, 697
CviQI GTAC 4 cut(s) 30, 396, 499, 589
DdeI CTNAG 2 cut(s) 253, 390
DpnI GATC 1 cut(s) 475
DpnII GATC 1 cut(s) 473
DraI TTTAAA 1 cut(s) 243
Eam1104I CTCTTC 1 cut(s) 32
EarI CTCTTC 1 cut(s) 32
EciI GGCGGA 1 cut(s) 370
Ecl136II GAGCTC 1 cut(s) 697
Eco24I GRGCYC 1 cut(s) 699
Eco31I GGTCTC 1 cut(s) 34
Eco47I GGWCC 1 cut(s) 450
Eco53kI GAGCTC 1 cut(s) 697
Eco57I CTGAAG 2 cut(s) 150, 584
EcoICRI GAGCTC 1 cut(s) 697
EcoNI CCTNNNNNAGG 1 cut(s) 520
EcoRII CCWGG 1 cut(s) 409
EcoT22I ATGCAT 1 cut(s) 303
EcoT38I GRGCYC 1 cut(s) 699
FaeI CATG 6 cut(s) 119, 156, 163, 631, 659, 703
FalI AAGNNNNNCTT 4 cut(s) 130, 162, 585, 617
FaqI GGGAC 1 cut(s) 590
FatI CATG 6 cut(s) 115, 152, 159, 627, 655, 699
FauI CCCGC 1 cut(s) 225
FokI GGATG 1 cut(s) 236
FriOI GRGCYC 1 cut(s) 699
FspBI CTAG 2 cut(s) 110, 275
FspI TGCGCA 1 cut(s) 654
GlaI GCGC 1 cut(s) 654
HapII CCGG 3 cut(s) 69, 448, 733
HhaI GCGC 1 cut(s) 655
Hin1II CATG 6 cut(s) 119, 156, 163, 631, 659, 703
Hin6I GCGC 1 cut(s) 653
HinP1I GCGC 1 cut(s) 653
HindIII AAGCTT 1 cut(s) 599
HinfI GANTC 4 cut(s) 94, 377, 406, 440
HpaII CCGG 3 cut(s) 69, 448, 733
HphI GGTGA 2 cut(s) 274, 278
Hpy166II GTNNAC 4 cut(s) 180, 282, 499, 724
Hpy188I TCNGA 1 cut(s) 169
Hpy188III TCNNGA 4 cut(s) 481, 562, 700, 733
Hpy8I GTNNAC 4 cut(s) 180, 282, 499, 724
HpyAV CCTTC 9 cut(s) 54, 200, 253, 257, 284, 526, 618, 623, 634
HpyCH4III ACNGT 2 cut(s) 87, 184
HpyCH4IV ACGT 1 cut(s) 313
HpyCH4V TGCA 3 cut(s) 301, 336, 539
HpyF10VI GCNNNNNNNGC 1 cut(s) 342
HpyF3I CTNAG 2 cut(s) 253, 390
HpySE526I ACGT 1 cut(s) 313
Hsp92II CATG 6 cut(s) 119, 156, 163, 631, 659, 703
HspAI GCGC 1 cut(s) 653
Kpn2I TCCGGA 1 cut(s) 732
Kzo9I GATC 1 cut(s) 473
LpnPI CCDG 9 cut(s) 82, 147, 396, 399, 423, 461, 525, 558, 575
LweI GCATC 2 cut(s) 288, 538
MaeI CTAG 2 cut(s) 110, 275
MaeII ACGT 1 cut(s) 313
MaeIII GTNAC 2 cut(s) 71, 266
MalI GATC 1 cut(s) 475
MboI GATC 1 cut(s) 473
MboII GAAGA 6 cut(s) 43, 49, 56, 156, 386, 577
MflI RGATCY 1 cut(s) 473
MhlI GDGCHC 1 cut(s) 699
MluCI AATT 2 cut(s) 328, 504
MmeI TCCRAC 1 cut(s) 655
MnlI CCTC 3 cut(s) 84, 335, 471
Mph1103I ATGCAT 1 cut(s) 303
MroI TCCGGA 1 cut(s) 732
MseI TTAA 1 cut(s) 242
MspA1I CMGCKG 1 cut(s) 437
MspI CCGG 3 cut(s) 69, 448, 733
MspR9I CCNGG 2 cut(s) 69, 411
MvaI CCWGG 1 cut(s) 411
MwoI GCNNNNNNNGC 1 cut(s) 342
NciI CCSGG 1 cut(s) 69
NdeII GATC 1 cut(s) 473
NlaIII CATG 6 cut(s) 119, 156, 163, 631, 659, 703
NlaIV GGNNCC 1 cut(s) 580
NmuCI GTSAC 1 cut(s) 266
NsbI TGCGCA 1 cut(s) 654
NsiI ATGCAT 1 cut(s) 303
NspI RCATGY 1 cut(s) 163
PagI TCATGA 1 cut(s) 699
PciI ACATGT 1 cut(s) 159
PfeI GAWTC 4 cut(s) 94, 377, 406, 440
PflMI CCANNNNNTGG 1 cut(s) 670
Ppu21I YACGTR 1 cut(s) 314
PscI ACATGT 1 cut(s) 159
Psp124BI GAGCTC 1 cut(s) 699
Psp6I CCWGG 1 cut(s) 409
PspGI CCWGG 1 cut(s) 409
PspN4I GGNNCC 1 cut(s) 580
PspPI GGNCC 1 cut(s) 450
PstI CTGCAG 1 cut(s) 541
PsuI RGATCY 1 cut(s) 473
PvuII CAGCTG 1 cut(s) 437
RsaI GTAC 4 cut(s) 31, 397, 500, 590
RsaNI GTAC 4 cut(s) 30, 396, 499, 589
SacI GAGCTC 1 cut(s) 699
SaqAI TTAA 1 cut(s) 242
Sau3AI GATC 1 cut(s) 473
Sau96I GGNCC 1 cut(s) 450
ScaI AGTACT 1 cut(s) 397
ScrFI CCNGG 2 cut(s) 69, 411
SduI GDGCHC 1 cut(s) 699
SfaNI GCATC 2 cut(s) 288, 538
SfcI CTRYAG 2 cut(s) 520, 537
SinI GGWCC 1 cut(s) 450
Sse9I AATT 2 cut(s) 328, 504
SsiI CCGC 3 cut(s) 101, 232, 381
SspI AATATT 1 cut(s) 491
SspMI CTAG 2 cut(s) 110, 275
SstI GAGCTC 1 cut(s) 699
StyD4I CCNGG 2 cut(s) 67, 409
TaaI ACNGT 2 cut(s) 87, 184
TaiI ACGT 1 cut(s) 316
TaqI TCGA 1 cut(s) 693
TasI AATT 2 cut(s) 328, 504
TatI WGTACW 2 cut(s) 395, 498
TfiI GAWTC 4 cut(s) 94, 377, 406, 440
Tru1I TTAA 1 cut(s) 242
Tru9I TTAA 1 cut(s) 242
TscAI CASTG 2 cut(s) 85, 261
TseFI GTSAC 1 cut(s) 266
Tsp45I GTSAC 1 cut(s) 266
TspDTI ATGAA 6 cut(s) 295, 303, 378, 564, 616, 716
TspRI CASTG 2 cut(s) 85, 261
Van91I CCANNNNNTGG 1 cut(s) 670
VpaK11BI GGWCC 1 cut(s) 450
XagI CCTNNNNNAGG 1 cut(s) 520
XceI RCATGY 1 cut(s) 163
XcmI CCANNNNNNNNNTGG 1 cut(s) 671
XspI CTAG 2 cut(s) 110, 275
ZrmI AGTACT 1 cut(s) 397
Zsp2I ATGCAT 1 cut(s) 303
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.