Rroxscaffold_5G00367690

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000235
Physical Location & Seq
Forward (+)
49509359 .. 49511813
2455 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00367690.1

Sequence Viewer

Length: 825 bp
ATGGCGAGAAGAGCATGCACATCTCTTCCATTTCATGATGGTAGTGTTGAGGAGGAGAAGGGGCCTACCTTCTTCAAGATCATAAGGCCTGGATTCAACACTGACCATTTGAGAATCCCACCTGCATTTCGGAAGCATATATTACATGAATCCACTAAGAGGGCAACCTTGAAATTGAAGAATTCATCAGATACTTCCTGGACTGTTAAAATAAGTATAACAGGAAGGGATATATATTTGAAGGATGGCTGGCAGGAGTTTTTAAGAGATAACTCCCTAGGTGATTCCGAGTTCTTAGTGTTTAGGTATGACAAAAATATGCATTTCATCATTAATATTTTTGACAAGAATTGTGTTGAGAGAGTGAATATCACCGACCTCAGAACACATAAAGCATCCACCTTTCCAGACAATACTAAACCTTTGGCAAGACCACCTACAGCTGCAGGTGATCGTCCATATGACTCGGCTGCGGAAGCTGCTCATGTGCTTAAATCCAAAGGTGAGCACCTTCCAAGTTATGCAGCTGCCGCAGCTTTCAAATCCAAATTTCCTTACTTTGCCAAAGTTCTTGCAGGATCGAAGATGGTGGCAATTCCCACACACTTCTATAGGGAGATATTTTCGTCAAGCAAATTTGATAAACTTTTCCTGGTGACTTCAGTGGGAAAATTCACAGTGACGCTTCTCCCATGCACTGATACAGTGATGTTGTCTGGAGGGTGGTCTTCCTTTAGAAATGACAACCAACTCCAAGAAGATGATATTTGCATCTTTGAGCTTGTGAAAGAAAACACCATGCTAGTTCACATTTTCCGAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0007275 GO:0008150 GO:0009553 GO:0009566 GO:0009567 GO:0019953 GO:0022414 GO:0032501 GO:0032502 GO:0044703 GO:0048229 GO:0048856 GO:0051704
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

274

Amino Acids

31.15

Weight (kDa)

9.17

Isoelectric Point (pI)

45.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 24 - 117 1.1e-17 B3 DNA binding domain
B3 PF02362 194 - 274 1.5e-12 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000235)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g22700 FvH4_2g06920 FvH4_2g06920 FvH4_2g06960 FvH4_2g06960 FvH4_2g06960 FvH4_2g06960 FvH4_2g06970 FvH4_2g06970 FvH4_2g07230 FvH4_2g07231 FvH4_2g07231 FvH4_2g07240 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_4g19990 FvH4_4g19990 FvH4_4g19990 FvH4_4g20070
malus_domestica MD05G1072700.v1.1
prunus_persica Prupe.8G113100_v2.0.a1
pyrus_communis pycom05g06500 pycom10g06950
rosa_chinensis RchiOBHm_Chr4g0425711 RchiOBHm_Chr6g0259241 RchiOBHm_Chr6g0259251 RchiOBHm_Chr6g0259321 RchiOBHm_Chr6g0259331 RchiOBHm_Chr6g0259781 RchiOBHm_Chr6g0259791 RchiOBHm_Chr6g0259811 RchiOBHm_Chr6g0259881 RchiOBHm_Chr6g0259931
rosa_laevigata RLG00000007345 RLG00000014500 RLG00000014501 RLG00000014505 RLG00000014506 RLG00000014507 RLG00000014539 RLG00000014540 RLG00000014545 RLG00000014546
rosa_multiflora Rmu_co8137334.1_g000001 Rmu_co8454279.1_g000001 Rmu_co8476373.1_g000001 Rmu_sc0001078.1_g000001 Rmu_sc0003958.1_g000011 Rmu_sc0009049.1_g000001 Rmu_sc0009049.1_g000009 Rmu_sc0010546.1_g000001 Rmu_sc0011974.1_g000002 Rmu_sc0011974.1_g000003 Rmu_sc0033846.1_g000001 Rmu_sc0036999.1_g000001 Rmu_ssc0000052.1_g000015
rosa_roxburghii Rroxscaffold_5G00367690 Rroxscaffold_7G00206530 Rroxscaffold_7G00206540 Rroxscaffold_7G00206560 Rroxscaffold_7G00206570 Rroxscaffold_7G00207020 Rroxscaffold_7G00207030 Rroxscaffold_7G00207150 Rroxscaffold_7G00207160 Rroxscaffold_7G00207170 Rroxscaffold_7G00207270
rosa_rugosa Rorug04G0205300 Rorug05G0586300 Rorug05G0586400 Rorug05G0586500 Rorug05G0586600 Rorug05G0586700 Rorug05G0587300 Rorug05G0587400 Rorug05G0587500 Rorug05G0587600 Rorug05G0590500 Rorug05G0590600 Rorug05G0591200 Rorug05G0591300 Rorug05G0591400 Rorug05G0591500
rosa_samantha Rh4AG262200 Rh4BG268500 Rh4CG279700 Rh4DG264100 Rh6AG103100 Rh6AG103600 Rh6AG107100 Rh6AG107200 Rh6AG107700 Rh6BG095400 Rh6BG095500 Rh6BG095900 Rh6BG096000 Rh6BG096800 Rh6BG102600 Rh6BG102700 Rh6BG102800 Rh6BG103800 Rh6CG092300 Rh6CG092400 Rh6CG092800 Rh6CG092900 Rh6CG096200 Rh6CG096300 Rh6CG096800 Rh6CG097100 Rh6DG086300 Rh6DG086400 Rh6DG086700 Rh6DG086800 Rh6DG090000 Rh6DG090100 Rh6DG090500 Rh6DG090800
rosa_wichuraiana Rw4G022670 Rw6G008900 Rw6G008940 Rw6G009250 Rw6G009280 Rw6G009330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 130, 437
Acc36I ACCTGC 2 cut(s) 130, 437
AciI CCGC 2 cut(s) 473, 531
AclWI GGATC 1 cut(s) 586
AcsI RAATTY 4 cut(s) 181, 548, 635, 671
AcuI CTGAAG 1 cut(s) 645
AfiI CCNNNNNNNGG 1 cut(s) 159
AgsI TTSAA 6 cut(s) 76, 97, 172, 178, 241, 541
AjnI CCWGG 3 cut(s) 88, 197, 651
AluBI AGCT 5 cut(s) 443, 479, 527, 536, 781
AluI AGCT 5 cut(s) 443, 479, 527, 536, 781
Alw21I GWGCWC 1 cut(s) 510
AlwI GGATC 1 cut(s) 586
AoxI GGCC 2 cut(s) 62, 86
ApeKI GCWGC 6 cut(s) 443, 470, 479, 524, 527, 533
ApoI RAATTY 4 cut(s) 181, 548, 635, 671
AseI ATTAAT 1 cut(s) 333
AspA2I CCTAGG 1 cut(s) 277
AspS9I GGNCC 1 cut(s) 62
AsuHPI GGTGA 5 cut(s) 293, 364, 461, 515, 667
AvrII CCTAGG 1 cut(s) 277
BbsI GAAGAC 1 cut(s) 720
Bbv12I GWGCWC 1 cut(s) 510
BbvI GCAGC 6 cut(s) 430, 457, 466, 514, 536, 545
BccI CCATC 3 cut(s) 32, 239, 580
BciT130I CCWGG 3 cut(s) 90, 199, 653
BfaI CTAG 2 cut(s) 278, 803
BfmI CTRYAG 3 cut(s) 438, 444, 610
BfuAI ACCTGC 2 cut(s) 130, 437
BisI GCNGC 7 cut(s) 444, 471, 480, 525, 528, 531, 534
BlnI CCTAGG 1 cut(s) 277
BlsI GCNGC 7 cut(s) 445, 472, 481, 526, 529, 532, 535
Bme1390I CCNGG 3 cut(s) 90, 199, 653
BmgT120I GGNCC 1 cut(s) 62
BmiI GGNNCC 1 cut(s) 63
BmrFI CCNGG 3 cut(s) 90, 199, 653
BmsI GCATC 2 cut(s) 404, 780
BpiI GAAGAC 1 cut(s) 720
BpmI CTGGAG 1 cut(s) 738
BsaJI CCNNGG 1 cut(s) 277
Bsc4I CCNNNNNNNGG 1 cut(s) 159
BseBI CCWGG 3 cut(s) 90, 199, 653
BseDI CCNNGG 1 cut(s) 277
BseGI GGATG 2 cut(s) 250, 395
BseLI CCNNNNNNNGG 1 cut(s) 159
BseMII CTCAG 1 cut(s) 394
BseRI GAGGAG 2 cut(s) 65, 68
BseXI GCAGC 6 cut(s) 430, 457, 466, 514, 536, 545
BshFI GGCC 2 cut(s) 64, 88
BsiHKAI GWGCWC 1 cut(s) 510
BslI CCNNNNNNNGG 1 cut(s) 159
BsnI GGCC 2 cut(s) 64, 88
Bsp1286I GDGCHC 1 cut(s) 510
Bsp143I GATC 3 cut(s) 78, 451, 578
BspACI CCGC 2 cut(s) 473, 531
BspANI GGCC 2 cut(s) 64, 88
BspCNI CTCAG 1 cut(s) 393
BspHI TCATGA 1 cut(s) 34
BspLI GGNNCC 1 cut(s) 63
BspMAI CTGCAG 1 cut(s) 448
BspMI ACCTGC 2 cut(s) 130, 437
BspPI GGATC 1 cut(s) 586
BspQI GCTCTTC 1 cut(s) 4
BssECI CCNNGG 1 cut(s) 277
BssMI GATC 3 cut(s) 78, 451, 578
BssT1I CCWWGG 1 cut(s) 277
Bst2UI CCWGG 3 cut(s) 90, 199, 653
Bst4CI ACNGT 3 cut(s) 205, 679, 706
Bst6I CTCTTC 2 cut(s) 4, 30
BstC8I GCNNGC 2 cut(s) 16, 251
BstDEI CTNAG 3 cut(s) 156, 295, 380
BstF5I GGATG 2 cut(s) 250, 395
BstKTI GATC 3 cut(s) 81, 454, 581
BstMBI GATC 3 cut(s) 78, 451, 578
BstMWI GCNNNNNNNGC 5 cut(s) 11, 476, 479, 530, 533
BstNI CCWGG 3 cut(s) 90, 199, 653
BstNSI RCATGY 1 cut(s) 18
BstSCI CCNGG 3 cut(s) 88, 197, 651
BstSFI CTRYAG 3 cut(s) 438, 444, 610
BstV1I GCAGC 6 cut(s) 430, 457, 466, 514, 536, 545
BstV2I GAAGAC 1 cut(s) 720
BsuRI GGCC 2 cut(s) 64, 88
BtsCI GGATG 2 cut(s) 250, 395
BtsIMutI CAGTG 5 cut(s) 99, 669, 684, 696, 711
BveI ACCTGC 2 cut(s) 130, 437
Cac8I GCNNGC 2 cut(s) 16, 251
CciI TCATGA 1 cut(s) 34
Cfr13I GGNCC 1 cut(s) 62
CseI GACGC 1 cut(s) 691
CviAII CATG 6 cut(s) 15, 35, 146, 485, 693, 799
CviJI RGCY 9 cut(s) 64, 88, 249, 443, 470, 479, 527, 536, 781
CviKI_1 RGCY 9 cut(s) 64, 88, 249, 443, 470, 479, 527, 536, 781
DdeI CTNAG 3 cut(s) 156, 295, 380
DpnI GATC 3 cut(s) 80, 453, 580
DpnII GATC 3 cut(s) 78, 451, 578
Eam1104I CTCTTC 2 cut(s) 4, 30
EarI CTCTTC 2 cut(s) 4, 30
Eco130I CCWWGG 1 cut(s) 277
Eco147I AGGCCT 1 cut(s) 88
Eco57I CTGAAG 1 cut(s) 645
EcoO109I RGGNCCY 1 cut(s) 62
EcoRI GAATTC 1 cut(s) 181
EcoRII CCWGG 3 cut(s) 88, 197, 651
EcoT14I CCWWGG 1 cut(s) 277
EcoT22I ATGCAT 1 cut(s) 324
ErhI CCWWGG 1 cut(s) 277
FaeI CATG 6 cut(s) 18, 38, 149, 488, 696, 802
FatI CATG 6 cut(s) 14, 34, 145, 484, 692, 798
FauNDI CATATG 1 cut(s) 460
Fnu4HI GCNGC 7 cut(s) 444, 471, 480, 525, 528, 531, 534
FokI GGATG 2 cut(s) 257, 382
Fsp4HI GCNGC 7 cut(s) 444, 471, 480, 525, 528, 531, 534
FspBI CTAG 2 cut(s) 278, 803
GluI GCNGC 7 cut(s) 444, 471, 480, 525, 528, 531, 534
GsuI CTGGAG 1 cut(s) 738
HaeIII GGCC 2 cut(s) 64, 88
HgaI GACGC 1 cut(s) 691
Hin1II CATG 6 cut(s) 18, 38, 149, 488, 696, 802
HinfI GANTC 5 cut(s) 93, 114, 149, 284, 464
HphI GGTGA 5 cut(s) 293, 364, 461, 515, 667
Hpy166II GTNNAC 1 cut(s) 808
Hpy188I TCNGA 5 cut(s) 132, 190, 289, 383, 818
Hpy188III TCNNGA 4 cut(s) 35, 76, 407, 717
Hpy8I GTNNAC 1 cut(s) 808
HpyAV CCTTC 5 cut(s) 52, 79, 219, 235, 521
HpyCH4III ACNGT 3 cut(s) 205, 679, 706
HpyCH4V TGCA 8 cut(s) 18, 125, 322, 446, 524, 575, 696, 771
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 476, 479, 530, 533
HpyF3I CTNAG 3 cut(s) 156, 295, 380
Hsp92II CATG 6 cut(s) 18, 38, 149, 488, 696, 802
Kzo9I GATC 3 cut(s) 78, 451, 578
LguI GCTCTTC 1 cut(s) 4
Lsp1109I GCAGC 6 cut(s) 430, 457, 466, 514, 536, 545
LweI GCATC 2 cut(s) 404, 780
MaeI CTAG 2 cut(s) 278, 803
MaeIII GTNAC 2 cut(s) 655, 679
MalI GATC 3 cut(s) 80, 453, 580
MboI GATC 3 cut(s) 78, 451, 578
MboII GAAGA 7 cut(s) 17, 21, 64, 190, 595, 720, 770
MhlI GDGCHC 1 cut(s) 510
MluCI AATT 7 cut(s) 173, 181, 349, 548, 594, 635, 671
MlyI GAGTC 1 cut(s) 458
MnlI CCTC 5 cut(s) 43, 46, 153, 389, 713
Mph1103I ATGCAT 1 cut(s) 324
MseI TTAA 4 cut(s) 207, 263, 333, 492
MspA1I CMGCKG 2 cut(s) 443, 527
MspR9I CCNGG 3 cut(s) 90, 199, 653
MvaI CCWGG 3 cut(s) 90, 199, 653
MwoI GCNNNNNNNGC 5 cut(s) 11, 476, 479, 530, 533
NdeI CATATG 1 cut(s) 460
NdeII GATC 3 cut(s) 78, 451, 578
NlaIII CATG 6 cut(s) 18, 38, 149, 488, 696, 802
NlaIV GGNNCC 1 cut(s) 63
NmeAIII GCCGAG 1 cut(s) 446
NmuCI GTSAC 2 cut(s) 655, 679
NsiI ATGCAT 1 cut(s) 324
NspI RCATGY 1 cut(s) 18
PaeI GCATGC 1 cut(s) 18
PagI TCATGA 1 cut(s) 34
PaqCI CACCTGC 2 cut(s) 130, 437
PceI AGGCCT 1 cut(s) 88
PciSI GCTCTTC 1 cut(s) 4
PfeI GAWTC 4 cut(s) 93, 114, 149, 284
PfoI TCCNGGA 1 cut(s) 197
PkrI GCNGC 7 cut(s) 445, 472, 481, 526, 529, 532, 535
PleI GAGTC 1 cut(s) 458
PpsI GAGTC 1 cut(s) 458
PshBI ATTAAT 1 cut(s) 333
Psp6I CCWGG 3 cut(s) 88, 197, 651
PspGI CCWGG 3 cut(s) 88, 197, 651
PspN4I GGNNCC 1 cut(s) 63
PspPI GGNCC 1 cut(s) 62
PstI CTGCAG 1 cut(s) 448
PvuII CAGCTG 2 cut(s) 443, 527
SapI GCTCTTC 1 cut(s) 4
SaqAI TTAA 4 cut(s) 207, 263, 333, 492
SatI GCNGC 7 cut(s) 444, 471, 480, 525, 528, 531, 534
Sau3AI GATC 3 cut(s) 78, 451, 578
Sau96I GGNCC 1 cut(s) 62
SchI GAGTC 1 cut(s) 458
ScrFI CCNGG 3 cut(s) 90, 199, 653
SduI GDGCHC 1 cut(s) 510
SfaNI GCATC 2 cut(s) 404, 780
SfcI CTRYAG 3 cut(s) 438, 444, 610
SphI GCATGC 1 cut(s) 18
Sse9I AATT 7 cut(s) 173, 181, 349, 548, 594, 635, 671
SseBI AGGCCT 1 cut(s) 88
SsiI CCGC 2 cut(s) 473, 531
SspI AATATT 1 cut(s) 337
SspMI CTAG 2 cut(s) 278, 803
StuI AGGCCT 1 cut(s) 88
StyD4I CCNGG 3 cut(s) 88, 197, 651
StyI CCWWGG 1 cut(s) 277
TaaI ACNGT 3 cut(s) 205, 679, 706
TaqI TCGA 1 cut(s) 581
TasI AATT 7 cut(s) 173, 181, 349, 548, 594, 635, 671
TauI GCSGC 1 cut(s) 533
TfiI GAWTC 4 cut(s) 93, 114, 149, 284
Tru1I TTAA 4 cut(s) 207, 263, 333, 492
Tru9I TTAA 4 cut(s) 207, 263, 333, 492
TscAI CASTG 5 cut(s) 106, 669, 684, 703, 711
TseFI GTSAC 2 cut(s) 655, 679
TseI GCWGC 6 cut(s) 443, 470, 479, 524, 527, 533
Tsp45I GTSAC 2 cut(s) 655, 679
TspDTI ATGAA 4 cut(s) 23, 162, 174, 316
TspRI CASTG 5 cut(s) 106, 669, 684, 703, 711
VspI ATTAAT 1 cut(s) 333
XapI RAATTY 4 cut(s) 181, 548, 635, 671
XceI RCATGY 1 cut(s) 18
XmaJI CCTAGG 1 cut(s) 277
XspI CTAG 2 cut(s) 278, 803
Zsp2I ATGCAT 1 cut(s) 324
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.