MD05G1072700.v1.1

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000235
Physical Location & Seq
Reverse (-)
15378776 .. 15381129
2354 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1072700.v1.1.491

Sequence Viewer

Length: 615 bp
ATGGCAAGAATGCCCAAGGAGGAGAAGAAGCCTACCTTCTTTCAGATTATTTGCAAAGGTTTCAACACTGAAAAATTGACTGTCCCACGAAAGTTCTTGACACATATACTGAAGGAGCTGTCCGAGAGAGCAAGTTTGAAACTGAAAGGTTCTTCAGATTACTCATGGACTGTCAATGTGACTAAAACACCAAAAGGTGCGGTGCGTTTCAATGGTGGCTGGCAGGAGTTCTTGAAGGATAACTCCTTGGGTCACGGCGACTTTTTGGTGTTCACTTATGATGGAAAGATGCAATTCAGCATAGATATATTCGACAACACTGCATGTCACAGAATCGATGACAAGATCAACCCCAATTCAAACAGCTCTTCTGGTGACGCTTCGGTTCAAAACCTTTCAAGTTCCGAATCCAGTGAGTTTGATGAGAGTATAGAAGAGGAAGAAGATGACAGTTGCAAGGACTCGGAAGCTACCTGTGAAGACAACATATCTGAGGATTCAGAAGCTACACGGACTGAATCTTTCAAATCTAACTTTCCTCATTACAATGACACATATTCGTCCCTTCAGCCTTCTCCAGGAAGCATTTCCCCCAAGGGAAAAGTTTTGAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0007275 GO:0008150 GO:0009553 GO:0009566 GO:0009567 GO:0019953 GO:0022414 GO:0032501 GO:0032502 GO:0044703 GO:0048229 GO:0048856 GO:0051704
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

205

Amino Acids

22.91

Weight (kDa)

5.02

Isoelectric Point (pI)

59.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 21 - 106 3.4e-16 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000235)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g22700 FvH4_2g06920 FvH4_2g06920 FvH4_2g06960 FvH4_2g06960 FvH4_2g06960 FvH4_2g06960 FvH4_2g06970 FvH4_2g06970 FvH4_2g07230 FvH4_2g07231 FvH4_2g07231 FvH4_2g07240 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_4g19990 FvH4_4g19990 FvH4_4g19990 FvH4_4g20070
malus_domestica MD05G1072700.v1.1
prunus_persica Prupe.8G113100_v2.0.a1
pyrus_communis pycom05g06500 pycom10g06950
rosa_chinensis RchiOBHm_Chr4g0425711 RchiOBHm_Chr6g0259241 RchiOBHm_Chr6g0259251 RchiOBHm_Chr6g0259321 RchiOBHm_Chr6g0259331 RchiOBHm_Chr6g0259781 RchiOBHm_Chr6g0259791 RchiOBHm_Chr6g0259811 RchiOBHm_Chr6g0259881 RchiOBHm_Chr6g0259931
rosa_laevigata RLG00000007345 RLG00000014500 RLG00000014501 RLG00000014505 RLG00000014506 RLG00000014507 RLG00000014539 RLG00000014540 RLG00000014545 RLG00000014546
rosa_multiflora Rmu_co8137334.1_g000001 Rmu_co8454279.1_g000001 Rmu_co8476373.1_g000001 Rmu_sc0001078.1_g000001 Rmu_sc0003958.1_g000011 Rmu_sc0009049.1_g000001 Rmu_sc0009049.1_g000009 Rmu_sc0010546.1_g000001 Rmu_sc0011974.1_g000002 Rmu_sc0011974.1_g000003 Rmu_sc0033846.1_g000001 Rmu_sc0036999.1_g000001 Rmu_ssc0000052.1_g000015
rosa_roxburghii Rroxscaffold_5G00367690 Rroxscaffold_7G00206530 Rroxscaffold_7G00206540 Rroxscaffold_7G00206560 Rroxscaffold_7G00206570 Rroxscaffold_7G00207020 Rroxscaffold_7G00207030 Rroxscaffold_7G00207150 Rroxscaffold_7G00207160 Rroxscaffold_7G00207170 Rroxscaffold_7G00207270
rosa_rugosa Rorug04G0205300 Rorug05G0586300 Rorug05G0586400 Rorug05G0586500 Rorug05G0586600 Rorug05G0586700 Rorug05G0587300 Rorug05G0587400 Rorug05G0587500 Rorug05G0587600 Rorug05G0590500 Rorug05G0590600 Rorug05G0591200 Rorug05G0591300 Rorug05G0591400 Rorug05G0591500
rosa_samantha Rh4AG262200 Rh4BG268500 Rh4CG279700 Rh4DG264100 Rh6AG103100 Rh6AG103600 Rh6AG107100 Rh6AG107200 Rh6AG107700 Rh6BG095400 Rh6BG095500 Rh6BG095900 Rh6BG096000 Rh6BG096800 Rh6BG102600 Rh6BG102700 Rh6BG102800 Rh6BG103800 Rh6CG092300 Rh6CG092400 Rh6CG092800 Rh6CG092900 Rh6CG096200 Rh6CG096300 Rh6CG096800 Rh6CG097100 Rh6DG086300 Rh6DG086400 Rh6DG086700 Rh6DG086800 Rh6DG090000 Rh6DG090100 Rh6DG090500 Rh6DG090800
rosa_wichuraiana Rw4G022670 Rw6G008900 Rw6G008940 Rw6G009250 Rw6G009280 Rw6G009330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 200
AcuI CTGAAG 3 cut(s) 131, 138, 551
AfiI CCNNNNNNNGG 1 cut(s) 578
AgsI TTSAA 8 cut(s) 64, 139, 211, 235, 360, 389, 399, 526
AjnI CCWGG 1 cut(s) 577
AluBI AGCT 4 cut(s) 118, 366, 470, 506
AluI AGCT 4 cut(s) 118, 366, 470, 506
Asp700I GAANNNNTTC 1 cut(s) 586
AsuHPI GGTGA 1 cut(s) 386
BbsI GAAGAC 1 cut(s) 486
BccI CCATC 1 cut(s) 275
BceAI ACGGC 1 cut(s) 271
BcgI CGANNNNNNTGC 2 cut(s) 302, 336
BciT130I CCWGG 1 cut(s) 579
Bme1390I CCNGG 1 cut(s) 579
BmrFI CCNGG 1 cut(s) 579
BmsI GCATC 1 cut(s) 279
BpiI GAAGAC 1 cut(s) 486
BpmI CTGGAG 1 cut(s) 561
Bsa29I ATCGAT 1 cut(s) 336
BsaJI CCNNGG 3 cut(s) 15, 246, 594
Bsc4I CCNNNNNNNGG 1 cut(s) 578
Bse1I ACTGG 1 cut(s) 411
BseBI CCWGG 1 cut(s) 579
BseCI ATCGAT 1 cut(s) 336
BseDI CCNNGG 3 cut(s) 15, 246, 594
BseLI CCNNNNNNNGG 1 cut(s) 578
BseMII CTCAG 1 cut(s) 483
BseNI ACTGG 1 cut(s) 411
BseRI GAGGAG 1 cut(s) 35
BshVI ATCGAT 1 cut(s) 336
BslFI GGGAC 2 cut(s) 68, 547
BslI CCNNNNNNNGG 1 cut(s) 578
BsmFI GGGAC 2 cut(s) 68, 547
BsmI GAATGC 1 cut(s) 15
Bsp143I GATC 1 cut(s) 345
BspACI CCGC 1 cut(s) 200
BspCNI CTCAG 1 cut(s) 484
BspDI ATCGAT 1 cut(s) 336
BspQI GCTCTTC 1 cut(s) 373
BsrI ACTGG 1 cut(s) 411
BssECI CCNNGG 3 cut(s) 15, 246, 594
BssMI GATC 1 cut(s) 345
BssT1I CCWWGG 3 cut(s) 15, 246, 594
Bst2UI CCWGG 1 cut(s) 579
Bst4CI ACNGT 3 cut(s) 82, 172, 452
Bst6I CTCTTC 2 cut(s) 373, 429
BstC8I GCNNGC 1 cut(s) 221
BstDEI CTNAG 1 cut(s) 492
BstENI CCTNNNNNAGG 1 cut(s) 576
BstKTI GATC 1 cut(s) 348
BstMBI GATC 1 cut(s) 345
BstNI CCWGG 1 cut(s) 579
BstNSI RCATGY 1 cut(s) 327
BstSCI CCNGG 1 cut(s) 577
BstV2I GAAGAC 1 cut(s) 486
Bsu15I ATCGAT 1 cut(s) 336
BsuTUI ATCGAT 1 cut(s) 336
BtsI GCAGTG 1 cut(s) 318
BtsIMutI CAGTG 3 cut(s) 66, 318, 418
Cac8I GCNNGC 1 cut(s) 221
ClaI ATCGAT 1 cut(s) 336
CseI GACGC 1 cut(s) 386
CviAII CATG 2 cut(s) 165, 324
CviJI RGCY 7 cut(s) 31, 118, 219, 366, 470, 506, 571
CviKI_1 RGCY 7 cut(s) 31, 118, 219, 366, 470, 506, 571
DdeI CTNAG 1 cut(s) 492
DpnI GATC 1 cut(s) 347
DpnII GATC 1 cut(s) 345
Eam1104I CTCTTC 2 cut(s) 373, 429
EarI CTCTTC 2 cut(s) 373, 429
Eco130I CCWWGG 3 cut(s) 15, 246, 594
Eco57I CTGAAG 3 cut(s) 131, 138, 551
EcoNI CCTNNNNNAGG 1 cut(s) 576
EcoRII CCWGG 1 cut(s) 577
EcoT14I CCWWGG 3 cut(s) 15, 246, 594
ErhI CCWWGG 3 cut(s) 15, 246, 594
FaeI CATG 2 cut(s) 168, 327
FalI AAGNNNNNCTT 2 cut(s) 20, 52
FaqI GGGAC 2 cut(s) 68, 547
FatI CATG 2 cut(s) 164, 323
GsuI CTGGAG 1 cut(s) 561
HgaI GACGC 1 cut(s) 386
Hin1II CATG 2 cut(s) 168, 327
HinfI GANTC 5 cut(s) 333, 407, 461, 497, 518
HphI GGTGA 1 cut(s) 386
Hpy166II GTNNAC 1 cut(s) 273
Hpy188I TCNGA 7 cut(s) 45, 124, 157, 406, 466, 493, 502
Hpy188III TCNNGA 2 cut(s) 97, 232
Hpy8I GTNNAC 1 cut(s) 273
HpyAV CCTTC 5 cut(s) 46, 106, 229, 575, 582
HpyCH4III ACNGT 3 cut(s) 82, 172, 452
HpyCH4V TGCA 4 cut(s) 54, 292, 323, 456
HpyF3I CTNAG 1 cut(s) 492
Hsp92II CATG 2 cut(s) 168, 327
Kzo9I GATC 1 cut(s) 345
LguI GCTCTTC 1 cut(s) 373
LmnI GCTCC 1 cut(s) 115
LpnPI CCDG 7 cut(s) 205, 209, 357, 424, 487, 564, 591
LweI GCATC 1 cut(s) 279
MaeIII GTNAC 4 cut(s) 178, 251, 326, 374
MalI GATC 1 cut(s) 347
MboI GATC 1 cut(s) 345
MboII GAAGA 7 cut(s) 37, 144, 360, 446, 452, 455, 491
MluCI AATT 3 cut(s) 74, 293, 355
MlyI GAGTC 1 cut(s) 455
MnlI CCTC 5 cut(s) 13, 430, 487, 549, 603
MroXI GAANNNNTTC 1 cut(s) 586
MslI CAYNNNNRTG 1 cut(s) 546
MspR9I CCNGG 1 cut(s) 579
Mva1269I GAATGC 1 cut(s) 15
MvaI CCWGG 1 cut(s) 579
NdeII GATC 1 cut(s) 345
NlaIII CATG 2 cut(s) 168, 327
NmuCI GTSAC 4 cut(s) 178, 251, 326, 374
NspI RCATGY 1 cut(s) 327
PciSI GCTCTTC 1 cut(s) 373
PctI GAATGC 1 cut(s) 15
PdmI GAANNNNTTC 1 cut(s) 586
PfeI GAWTC 4 cut(s) 333, 407, 497, 518
PfoI TCCNGGA 1 cut(s) 577
PleI GAGTC 1 cut(s) 455
PpsI GAGTC 1 cut(s) 455
Psp6I CCWGG 1 cut(s) 577
PspGI CCWGG 1 cut(s) 577
RseI CAYNNNNRTG 1 cut(s) 546
SapI GCTCTTC 1 cut(s) 373
Sau3AI GATC 1 cut(s) 345
SchI GAGTC 1 cut(s) 455
ScrFI CCNGG 1 cut(s) 579
SfaNI GCATC 1 cut(s) 279
SmiMI CAYNNNNRTG 1 cut(s) 546
Sse9I AATT 3 cut(s) 74, 293, 355
SsiI CCGC 1 cut(s) 200
StyD4I CCNGG 1 cut(s) 577
StyI CCWWGG 3 cut(s) 15, 246, 594
TaaI ACNGT 3 cut(s) 82, 172, 452
TaqI TCGA 2 cut(s) 312, 336
TasI AATT 3 cut(s) 74, 293, 355
TfiI GAWTC 4 cut(s) 333, 407, 497, 518
TscAI CASTG 3 cut(s) 73, 325, 418
TseFI GTSAC 4 cut(s) 178, 251, 326, 374
Tsp45I GTSAC 4 cut(s) 178, 251, 326, 374
TspGWI ACGGA 1 cut(s) 526
TspRI CASTG 3 cut(s) 73, 325, 418
XagI CCTNNNNNAGG 1 cut(s) 576
XceI RCATGY 1 cut(s) 327
XmnI GAANNNNTTC 1 cut(s) 586
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.