Rroxscaffold_7G00206570

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000235
Physical Location & Seq
Reverse (-)
56367148 .. 56369594
2447 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00206570.1

Sequence Viewer

Length: 765 bp
ATGGAGGGAGCATCCAAGAAGCCTACCTTCTTCAAGATAATCAAAACTGGGTTTAACACTGATGATCTGAGAATCCCCCCAAAGTTCCGGAGGCACATGTTAAATGAACTTTCTGAAACGGCAACTTTGGAGTTGAATGGTTCTTCAAAATGTTCATGGACTGTTCAAGTGAGTCAAATAGAAAGCGATATATATTTCAAGAATGGATGGCAGAAGTTTATAAAAGATAACTCCTTGGGCGATTTTGAGTTTTTAGTATTTAGGTATGAGAGACCGATGCATTTTACTATAGATATCTTTGACAAGACTGCATGCATGAGAAACAATCTTCTGGAAAAATCCACTAGGGCTGACAACACTAAGAGGCCTCGGGGTAGACCTAGAAACTGCAATCGTTCAGCTGCTGGTGAAAACATTCAAAGTTGCCAGTCCTTTGAACTGAAAGAGATCAAAGAAGAGGAAGCTGCCACATTGCCCAGTTCTGGGTGCCGTTCTTTTACAAGTATTATGGGGAAACACTGTTATAATGTGGCAGTTCCAGTGGACTTCAGTAGGGAGCATCTTCCTCAGGGCTACTACAACATTGTGCTGAAAAATTCGAAGGGACAAAAATGGAAAGTGAACGTCGTCTGTTCTGGTAATACAGTTAAACTTTCTGCAGGGTGGGTGGTGTTTAGACGTGCTAATCAAATTAACTGCGGAGATATTTGCACATTTGATCTTGTGGGAAGAAGGACCATGGTGGTTCACGTTATTCCAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0007275 GO:0008150 GO:0009553 GO:0009566 GO:0009567 GO:0019953 GO:0022414 GO:0032501 GO:0032502 GO:0044703 GO:0048229 GO:0048856 GO:0051704
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

254

Amino Acids

29.07

Weight (kDa)

9.42

Isoelectric Point (pI)

42.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 10 - 102 5.2e-15 B3 DNA binding domain
B3 PF02362 176 - 252 8.7e-13 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000235)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g22700 FvH4_2g06920 FvH4_2g06920 FvH4_2g06960 FvH4_2g06960 FvH4_2g06960 FvH4_2g06960 FvH4_2g06970 FvH4_2g06970 FvH4_2g07230 FvH4_2g07231 FvH4_2g07231 FvH4_2g07240 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_4g19990 FvH4_4g19990 FvH4_4g19990 FvH4_4g20070
malus_domestica MD05G1072700.v1.1
prunus_persica Prupe.8G113100_v2.0.a1
pyrus_communis pycom05g06500 pycom10g06950
rosa_chinensis RchiOBHm_Chr4g0425711 RchiOBHm_Chr6g0259241 RchiOBHm_Chr6g0259251 RchiOBHm_Chr6g0259321 RchiOBHm_Chr6g0259331 RchiOBHm_Chr6g0259781 RchiOBHm_Chr6g0259791 RchiOBHm_Chr6g0259811 RchiOBHm_Chr6g0259881 RchiOBHm_Chr6g0259931
rosa_laevigata RLG00000007345 RLG00000014500 RLG00000014501 RLG00000014505 RLG00000014506 RLG00000014507 RLG00000014539 RLG00000014540 RLG00000014545 RLG00000014546
rosa_multiflora Rmu_co8137334.1_g000001 Rmu_co8454279.1_g000001 Rmu_co8476373.1_g000001 Rmu_sc0001078.1_g000001 Rmu_sc0003958.1_g000011 Rmu_sc0009049.1_g000001 Rmu_sc0009049.1_g000009 Rmu_sc0010546.1_g000001 Rmu_sc0011974.1_g000002 Rmu_sc0011974.1_g000003 Rmu_sc0033846.1_g000001 Rmu_sc0036999.1_g000001 Rmu_ssc0000052.1_g000015
rosa_roxburghii Rroxscaffold_5G00367690 Rroxscaffold_7G00206530 Rroxscaffold_7G00206540 Rroxscaffold_7G00206560 Rroxscaffold_7G00206570 Rroxscaffold_7G00207020 Rroxscaffold_7G00207030 Rroxscaffold_7G00207150 Rroxscaffold_7G00207160 Rroxscaffold_7G00207170 Rroxscaffold_7G00207270
rosa_rugosa Rorug04G0205300 Rorug05G0586300 Rorug05G0586400 Rorug05G0586500 Rorug05G0586600 Rorug05G0586700 Rorug05G0587300 Rorug05G0587400 Rorug05G0587500 Rorug05G0587600 Rorug05G0590500 Rorug05G0590600 Rorug05G0591200 Rorug05G0591300 Rorug05G0591400 Rorug05G0591500
rosa_samantha Rh4AG262200 Rh4BG268500 Rh4CG279700 Rh4DG264100 Rh6AG103100 Rh6AG103600 Rh6AG107100 Rh6AG107200 Rh6AG107700 Rh6BG095400 Rh6BG095500 Rh6BG095900 Rh6BG096000 Rh6BG096800 Rh6BG102600 Rh6BG102700 Rh6BG102800 Rh6BG103800 Rh6CG092300 Rh6CG092400 Rh6CG092800 Rh6CG092900 Rh6CG096200 Rh6CG096300 Rh6CG096800 Rh6CG097100 Rh6DG086300 Rh6DG086400 Rh6DG086700 Rh6DG086800 Rh6DG090000 Rh6DG090100 Rh6DG090500 Rh6DG090800
rosa_wichuraiana Rw4G022670 Rw6G008900 Rw6G008940 Rw6G009250 Rw6G009280 Rw6G009330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 221, 525
AccB1I GGYRCC 1 cut(s) 486
AccI GTMKAC 1 cut(s) 376
AccIII TCCGGA 1 cut(s) 87
AciI CCGC 1 cut(s) 699
AcsI RAATTY 1 cut(s) 595
AcuI CTGAAG 1 cut(s) 532
AfiI CCNNNNNNNGG 2 cut(s) 482, 483
AflIII ACRYGT 1 cut(s) 96
AgsI TTSAA 7 cut(s) 34, 136, 147, 167, 199, 419, 437
AjiI CACGTC 1 cut(s) 680
AluBI AGCT 2 cut(s) 401, 464
AluI AGCT 2 cut(s) 401, 464
Alw26I GTCTC 1 cut(s) 265
AlwNI CAGNNNCTG 1 cut(s) 404
Ama87I CYCGRG 1 cut(s) 369
Aor13HI TCCGGA 1 cut(s) 87
AoxI GGCC 1 cut(s) 365
ApeKI GCWGC 2 cut(s) 401, 464
ApoI RAATTY 1 cut(s) 595
Asp700I GAANNNNTTC 1 cut(s) 414
AspS9I GGNCC 1 cut(s) 735
AsuHPI GGTGA 1 cut(s) 419
AsuII TTCGAA 1 cut(s) 599
AvaI CYCGRG 1 cut(s) 369
AvaII GGWCC 1 cut(s) 735
AxyI CCTNAGG 1 cut(s) 567
BanI GGYRCC 1 cut(s) 486
BbvI GCAGC 2 cut(s) 388, 451
BccI CCATC 1 cut(s) 201
BceAI ACGGC 2 cut(s) 135, 474
BcoDI GTCTC 1 cut(s) 265
BfaI CTAG 2 cut(s) 345, 381
BfmI CTRYAG 2 cut(s) 288, 657
BisI GCNGC 2 cut(s) 402, 465
BlsI GCNGC 2 cut(s) 403, 466
Bme18I GGWCC 1 cut(s) 735
BmeT110I CYCGRG 1 cut(s) 369
BmgBI CACGTC 1 cut(s) 680
BmgT120I GGNCC 1 cut(s) 735
BmiI GGNNCC 1 cut(s) 488
BmrI ACTGGG 2 cut(s) 57, 471
BmsI GCATC 3 cut(s) 20, 267, 568
BmuI ACTGGG 2 cut(s) 57, 471
Bpu14I TTCGAA 1 cut(s) 599
BsaI GGTCTC 1 cut(s) 265
BsaJI CCNNGG 3 cut(s) 234, 368, 738
BsaWI WCCGGW 1 cut(s) 87
Bsc4I CCNNNNNNNGG 2 cut(s) 482, 483
Bse1I ACTGG 4 cut(s) 52, 427, 477, 539
Bse21I CCTNAGG 1 cut(s) 567
Bse3DI GCAATG 1 cut(s) 470
BseAI TCCGGA 1 cut(s) 87
BseDI CCNNGG 3 cut(s) 234, 368, 738
BseGI GGATG 2 cut(s) 11, 212
BseLI CCNNNNNNNGG 2 cut(s) 482, 483
BseMI GCAATG 1 cut(s) 470
BseMII CTCAG 2 cut(s) 59, 581
BseNI ACTGG 4 cut(s) 52, 427, 477, 539
BseXI GCAGC 2 cut(s) 388, 451
BshFI GGCC 1 cut(s) 367
BshNI GGYRCC 1 cut(s) 486
BsiHKCI CYCGRG 1 cut(s) 369
BsiSI CCGG 1 cut(s) 88
BslFI GGGAC 1 cut(s) 618
BslI CCNNNNNNNGG 2 cut(s) 482, 483
BsmAI GTCTC 1 cut(s) 265
BsmFI GGGAC 1 cut(s) 618
BsnI GGCC 1 cut(s) 367
Bso31I GGTCTC 1 cut(s) 265
BsoBI CYCGRG 1 cut(s) 369
Bsp119I TTCGAA 1 cut(s) 599
Bsp13I TCCGGA 1 cut(s) 87
Bsp143I GATC 3 cut(s) 64, 447, 718
Bsp19I CCATGG 1 cut(s) 738
BspACI CCGC 1 cut(s) 699
BspANI GGCC 1 cut(s) 367
BspCNI CTCAG 2 cut(s) 60, 580
BspEI TCCGGA 1 cut(s) 87
BspLI GGNNCC 1 cut(s) 488
BspMAI CTGCAG 1 cut(s) 661
BspT104I TTCGAA 1 cut(s) 599
BspT107I GGYRCC 1 cut(s) 486
BspTNI GGTCTC 1 cut(s) 265
BsrDI GCAATG 1 cut(s) 470
BsrI ACTGG 4 cut(s) 52, 427, 477, 539
BssECI CCNNGG 3 cut(s) 234, 368, 738
BssMI GATC 3 cut(s) 64, 447, 718
BssT1I CCWWGG 2 cut(s) 234, 738
Bst4CI ACNGT 3 cut(s) 163, 521, 646
Bst6I CTCTTC 1 cut(s) 450
BstBI TTCGAA 1 cut(s) 599
BstC8I GCNNGC 1 cut(s) 313
BstDEI CTNAG 3 cut(s) 68, 360, 567
BstDSI CCRYGG 1 cut(s) 738
BstF5I GGATG 2 cut(s) 11, 212
BstKTI GATC 3 cut(s) 67, 450, 721
BstMAI GTCTC 1 cut(s) 265
BstMBI GATC 3 cut(s) 64, 447, 718
BstNSI RCATGY 2 cut(s) 100, 315
BstSFI CTRYAG 2 cut(s) 288, 657
BstV1I GCAGC 2 cut(s) 388, 451
Bsu36I CCTNAGG 1 cut(s) 567
BsuRI GGCC 1 cut(s) 367
BtgI CCRYGG 1 cut(s) 738
BtrI CACGTC 1 cut(s) 680
BtsCI GGATG 2 cut(s) 11, 212
BtsIMutI CAGTG 3 cut(s) 57, 517, 546
Cac8I GCNNGC 1 cut(s) 313
CaiI CAGNNNCTG 1 cut(s) 404
Cfr13I GGNCC 1 cut(s) 735
CviAII CATG 5 cut(s) 97, 156, 312, 316, 739
CviJI RGCY 6 cut(s) 22, 350, 367, 401, 464, 573
CviKI_1 RGCY 6 cut(s) 22, 350, 367, 401, 464, 573
DdeI CTNAG 3 cut(s) 68, 360, 567
DpnI GATC 3 cut(s) 66, 449, 720
DpnII GATC 3 cut(s) 64, 447, 718
Eam1104I CTCTTC 1 cut(s) 450
EarI CTCTTC 1 cut(s) 450
Eco130I CCWWGG 2 cut(s) 234, 738
Eco147I AGGCCT 1 cut(s) 367
Eco31I GGTCTC 1 cut(s) 265
Eco32I GATATC 1 cut(s) 295
Eco47I GGWCC 1 cut(s) 735
Eco57I CTGAAG 1 cut(s) 532
Eco81I CCTNAGG 1 cut(s) 567
Eco88I CYCGRG 1 cut(s) 369
EcoRV GATATC 1 cut(s) 295
EcoT14I CCWWGG 2 cut(s) 234, 738
EcoT22I ATGCAT 2 cut(s) 282, 317
ErhI CCWWGG 2 cut(s) 234, 738
FaeI CATG 5 cut(s) 100, 159, 315, 319, 742
FalI AAGNNNNNCTT 2 cut(s) 11, 43
FaqI GGGAC 1 cut(s) 618
FatI CATG 5 cut(s) 96, 155, 311, 315, 738
FblI GTMKAC 1 cut(s) 376
Fnu4HI GCNGC 2 cut(s) 402, 465
FokI GGATG 1 cut(s) 219
Fsp4HI GCNGC 2 cut(s) 402, 465
FspBI CTAG 2 cut(s) 345, 381
GluI GCNGC 2 cut(s) 402, 465
HaeIII GGCC 1 cut(s) 367
HapII CCGG 1 cut(s) 88
Hin1II CATG 5 cut(s) 100, 159, 315, 319, 742
HinfI GANTC 2 cut(s) 72, 172
HpaII CCGG 1 cut(s) 88
HphI GGTGA 1 cut(s) 419
Hpy166II GTNNAC 4 cut(s) 377, 544, 622, 748
Hpy188I TCNGA 2 cut(s) 69, 115
Hpy188III TCNNGA 4 cut(s) 34, 88, 199, 332
Hpy8I GTNNAC 4 cut(s) 377, 544, 622, 748
Hpy99I CGWCG 1 cut(s) 629
HpyAV CCTTC 3 cut(s) 37, 595, 726
HpyCH4III ACNGT 3 cut(s) 163, 521, 646
HpyCH4IV ACGT 3 cut(s) 624, 679, 750
HpyCH4V TGCA 6 cut(s) 280, 311, 315, 390, 659, 711
HpyF3I CTNAG 3 cut(s) 68, 360, 567
HpySE526I ACGT 3 cut(s) 624, 679, 750
Hsp92II CATG 5 cut(s) 100, 159, 315, 319, 742
Kpn2I TCCGGA 1 cut(s) 87
Kzo9I GATC 3 cut(s) 64, 447, 718
LmnI GCTCC 2 cut(s) 8, 556
Lsp1109I GCAGC 2 cut(s) 388, 451
LweI GCATC 3 cut(s) 20, 267, 568
MaeI CTAG 2 cut(s) 345, 381
MaeII ACGT 3 cut(s) 624, 679, 750
MalI GATC 3 cut(s) 66, 449, 720
MboI GATC 3 cut(s) 64, 447, 718
MboII GAAGA 6 cut(s) 22, 135, 320, 467, 554, 741
MluCI AATT 2 cut(s) 595, 690
MlyI GAGTC 1 cut(s) 181
MnlI CCTC 5 cut(s) 84, 357, 378, 451, 576
Mph1103I ATGCAT 2 cut(s) 282, 317
MroI TCCGGA 1 cut(s) 87
MroXI GAANNNNTTC 1 cut(s) 414
MseI TTAA 4 cut(s) 54, 101, 648, 693
MspA1I CMGCKG 1 cut(s) 401
MspI CCGG 1 cut(s) 88
NcoI CCATGG 1 cut(s) 738
NdeII GATC 3 cut(s) 64, 447, 718
NlaIII CATG 5 cut(s) 100, 159, 315, 319, 742
NlaIV GGNNCC 1 cut(s) 488
NsiI ATGCAT 2 cut(s) 282, 317
NspI RCATGY 2 cut(s) 100, 315
NspV TTCGAA 1 cut(s) 599
PaeI GCATGC 1 cut(s) 315
PceI AGGCCT 1 cut(s) 367
PciI ACATGT 1 cut(s) 96
PdmI GAANNNNTTC 1 cut(s) 414
PfeI GAWTC 1 cut(s) 72
PkrI GCNGC 2 cut(s) 403, 466
PleI GAGTC 1 cut(s) 180
PpsI GAGTC 1 cut(s) 180
PscI ACATGT 1 cut(s) 96
PsiI TTATAA 2 cut(s) 221, 525
PspN4I GGNNCC 1 cut(s) 488
PspPI GGNCC 1 cut(s) 735
PstI CTGCAG 1 cut(s) 661
PstNI CAGNNNCTG 1 cut(s) 404
PvuII CAGCTG 1 cut(s) 401
SaqAI TTAA 4 cut(s) 54, 101, 648, 693
SatI GCNGC 2 cut(s) 402, 465
Sau3AI GATC 3 cut(s) 64, 447, 718
Sau96I GGNCC 1 cut(s) 735
SchI GAGTC 1 cut(s) 181
SetI ASST 8 cut(s) 29, 266, 382, 403, 466, 627, 682, 753
SfaNI GCATC 3 cut(s) 20, 267, 568
SfcI CTRYAG 2 cut(s) 288, 657
SfuI TTCGAA 1 cut(s) 599
SinI GGWCC 1 cut(s) 735
SphI GCATGC 1 cut(s) 315
Sse9I AATT 2 cut(s) 595, 690
SseBI AGGCCT 1 cut(s) 367
SsiI CCGC 1 cut(s) 699
SspMI CTAG 2 cut(s) 345, 381
StuI AGGCCT 1 cut(s) 367
StyI CCWWGG 2 cut(s) 234, 738
TaaI ACNGT 3 cut(s) 163, 521, 646
TaiI ACGT 3 cut(s) 627, 682, 753
TaqI TCGA 1 cut(s) 599
TaqII GACCGA 1 cut(s) 289
TasI AATT 2 cut(s) 595, 690
TfiI GAWTC 1 cut(s) 72
Tru1I TTAA 4 cut(s) 54, 101, 648, 693
Tru9I TTAA 4 cut(s) 54, 101, 648, 693
TscAI CASTG 3 cut(s) 64, 524, 546
TseI GCWGC 2 cut(s) 401, 464
TspDTI ATGAA 2 cut(s) 120, 144
TspRI CASTG 3 cut(s) 64, 524, 546
VpaK11BI GGWCC 1 cut(s) 735
XapI RAATTY 1 cut(s) 595
XceI RCATGY 2 cut(s) 100, 315
XmiI GTMKAC 1 cut(s) 376
XmnI GAANNNNTTC 1 cut(s) 414
XspI CTAG 2 cut(s) 345, 381
Zsp2I ATGCAT 2 cut(s) 282, 317
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.