B3 domain-containing protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccBSI | CCGCTC | 3 cut(s) | 432, 510, 588 |
| AccI | GTMKAC | 8 cut(s) | 418, 496, 574, 641, 1549, 1574, 1594, 1729 |
| AccIII | TCCGGA | 1 cut(s) | 1122 |
| AciI | CCGC | 4 cut(s) | 432, 510, 588, 660 |
| AclWI | GGATC | 1 cut(s) | 1170 |
| AcsI | RAATTY | 5 cut(s) | 746, 940, 1780, 1787, 1969 |
| AcuI | CTGAAG | 1 cut(s) | 1923 |
| AcvI | CACGTG | 2 cut(s) | 413, 1752 |
| AcyI | GRCGYC | 1 cut(s) | 1585 |
| AdeI | CACNNNGTG | 1 cut(s) | 1135 |
| AfaI | GTAC | 9 cut(s) | 478, 1408, 1531, 1621, 1666, 1711, 1797, 1904, 1991 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 1185, 1401, 1922, 2020, 2071 |
| AflIII | ACRYGT | 1 cut(s) | 1751 |
| AjnI | CCWGG | 3 cut(s) | 753, 1028, 1078 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 340, 372 |
| AluBI | AGCT | 9 cut(s) | 356, 443, 521, 599, 886, 1059, 1457, 1465, 2002 |
| AluI | AGCT | 9 cut(s) | 356, 443, 521, 599, 886, 1059, 1457, 1465, 2002 |
| Alw21I | GWGCWC | 2 cut(s) | 888, 1445 |
| Alw26I | GTCTC | 2 cut(s) | 765, 1420 |
| AlwI | GGATC | 1 cut(s) | 1170 |
| Ama87I | CYCGRG | 1 cut(s) | 1762 |
| Aor13HI | TCCGGA | 1 cut(s) | 1122 |
| AoxI | GGCC | 4 cut(s) | 1415, 1628, 1673, 1718 |
| ApeKI | GCWGC | 3 cut(s) | 440, 518, 596 |
| ApoI | RAATTY | 5 cut(s) | 746, 940, 1780, 1787, 1969 |
| AseI | ATTAAT | 1 cut(s) | 678 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1539 |
| AspLEI | GCGC | 1 cut(s) | 1520 |
| AspS9I | GGNCC | 1 cut(s) | 47 |
| AsuC2I | CCSGG | 1 cut(s) | 75 |
| AvaI | CYCGRG | 1 cut(s) | 1762 |
| AvaII | GGWCC | 1 cut(s) | 47 |
| AxyI | CCTNAGG | 1 cut(s) | 262 |
| BanII | GRGCYC | 2 cut(s) | 199, 888 |
| BauI | CACGAG | 3 cut(s) | 489, 567, 1722 |
| BbrPI | CACGTG | 2 cut(s) | 413, 1752 |
| BbsI | GAAGAC | 1 cut(s) | 2017 |
| Bbv12I | GWGCWC | 2 cut(s) | 888, 1445 |
| BbvI | GCAGC | 3 cut(s) | 452, 530, 608 |
| BccI | CCATC | 6 cut(s) | 290, 771, 822, 898, 1232, 1236 |
| BciT130I | CCWGG | 3 cut(s) | 755, 1030, 1080 |
| BclI | TGATCA | 1 cut(s) | 918 |
| BcnI | CCSGG | 1 cut(s) | 75 |
| BcoDI | GTCTC | 2 cut(s) | 765, 1420 |
| BfaI | CTAG | 5 cut(s) | 798, 822, 1056, 1113, 1401 |
| BfmI | CTRYAG | 4 cut(s) | 171, 715, 1084, 1506 |
| BisI | GCNGC | 3 cut(s) | 441, 519, 597 |
| BlsI | GCNGC | 3 cut(s) | 442, 520, 598 |
| BmcAI | AGTACT | 5 cut(s) | 478, 1408, 1531, 1621, 1711 |
| Bme1390I | CCNGG | 4 cut(s) | 75, 755, 1030, 1080 |
| Bme18I | GGWCC | 1 cut(s) | 47 |
| BmeT110I | CYCGRG | 1 cut(s) | 1762 |
| BmgT120I | GGNCC | 1 cut(s) | 47 |
| BmiI | GGNNCC | 1 cut(s) | 48 |
| BmrFI | CCNGG | 4 cut(s) | 75, 755, 1030, 1080 |
| BmsI | GCATC | 6 cut(s) | 382, 427, 505, 583, 733, 1252 |
| BpiI | GAAGAC | 1 cut(s) | 2017 |
| BpmI | CTGGAG | 1 cut(s) | 872 |
| BpuEI | CTTGAG | 2 cut(s) | 209, 978 |
| BpuMI | CCSGG | 1 cut(s) | 75 |
| BsaAI | YACGTR | 2 cut(s) | 413, 1752 |
| BsaBI | GATNNNNATC | 1 cut(s) | 219 |
| BsaHI | GRCGYC | 1 cut(s) | 1585 |
| BsaI | GGTCTC | 1 cut(s) | 1420 |
| BsaWI | WCCGGW | 1 cut(s) | 1122 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 340, 370, 1209, 1239 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 1185, 1401, 1922, 2020, 2071 |
| Bse1I | ACTGG | 2 cut(s) | 468, 624 |
| Bse21I | CCTNAGG | 1 cut(s) | 262 |
| Bse8I | GATNNNNATC | 1 cut(s) | 219 |
| BseAI | TCCGGA | 1 cut(s) | 1122 |
| BseBI | CCWGG | 3 cut(s) | 755, 1030, 1080 |
| BseGI | GGATG | 3 cut(s) | 750, 833, 1247 |
| BseJI | GATNNNNATC | 1 cut(s) | 219 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 1185, 1401, 1922, 2020, 2071 |
| BseMII | CTCAG | 8 cut(s) | 86, 152, 1083, 1247, 1557, 1650, 1790, 1965 |
| BseNI | ACTGG | 2 cut(s) | 468, 624 |
| BseRI | GAGGAG | 4 cut(s) | 50, 1058, 1233, 1583 |
| BseXI | GCAGC | 3 cut(s) | 452, 530, 608 |
| BshFI | GGCC | 4 cut(s) | 1417, 1630, 1675, 1720 |
| BsiHKAI | GWGCWC | 2 cut(s) | 888, 1445 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1762 |
| BsiSI | CCGG | 2 cut(s) | 75, 1123 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 1185, 1401, 1922, 2020, 2071 |
| BsmAI | GTCTC | 2 cut(s) | 765, 1420 |
| BsmI | GAATGC | 1 cut(s) | 110 |
| BsnI | GGCC | 4 cut(s) | 1417, 1630, 1675, 1720 |
| Bso31I | GGTCTC | 1 cut(s) | 1420 |
| BsoBI | CYCGRG | 1 cut(s) | 1762 |
| Bsp1286I | GDGCHC | 3 cut(s) | 199, 888, 1445 |
| Bsp13I | TCCGGA | 1 cut(s) | 1122 |
| Bsp1407I | TGTACA | 1 cut(s) | 1902 |
| Bsp143I | GATC | 3 cut(s) | 88, 918, 1175 |
| BspACI | CCGC | 4 cut(s) | 432, 510, 588, 660 |
| BspANI | GGCC | 4 cut(s) | 1417, 1630, 1675, 1720 |
| BspCNI | CTCAG | 8 cut(s) | 87, 153, 1084, 1248, 1558, 1651, 1791, 1966 |
| BspEI | TCCGGA | 1 cut(s) | 1122 |
| BspHI | TCATGA | 1 cut(s) | 2028 |
| BspLI | GGNNCC | 1 cut(s) | 48 |
| BspMAI | CTGCAG | 1 cut(s) | 175 |
| BspPI | GGATC | 1 cut(s) | 1170 |
| BspTNI | GGTCTC | 1 cut(s) | 1420 |
| BsrBI | CCGCTC | 3 cut(s) | 432, 510, 588 |
| BsrGI | TGTACA | 1 cut(s) | 1902 |
| BsrI | ACTGG | 2 cut(s) | 468, 624 |
| BssMI | GATC | 3 cut(s) | 88, 918, 1175 |
| BssNAI | GTATAC | 2 cut(s) | 642, 1575 |
| BssNI | GRCGYC | 1 cut(s) | 1585 |
| BssSI | CACGAG | 3 cut(s) | 489, 567, 1722 |
| Bst1107I | GTATAC | 2 cut(s) | 642, 1575 |
| Bst2BI | CACGAG | 3 cut(s) | 489, 567, 1722 |
| Bst2UI | CCWGG | 3 cut(s) | 755, 1030, 1080 |
| Bst4CI | ACNGT | 4 cut(s) | 476, 1848, 1917, 2023 |
| Bst6I | CTCTTC | 2 cut(s) | 1476, 1809 |
| BstACI | GRCGYC | 1 cut(s) | 1585 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 2085 |
| BstAUI | TGTACA | 1 cut(s) | 1902 |
| BstBAI | YACGTR | 2 cut(s) | 413, 1752 |
| BstC8I | GCNNGC | 2 cut(s) | 236, 1267 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1399 |
| BstF5I | GGATG | 3 cut(s) | 750, 833, 1247 |
| BstHHI | GCGC | 1 cut(s) | 1520 |
| BstKTI | GATC | 3 cut(s) | 91, 921, 1178 |
| BstMAI | GTCTC | 2 cut(s) | 765, 1420 |
| BstMBI | GATC | 3 cut(s) | 88, 918, 1175 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 2085 |
| BstNI | CCWGG | 3 cut(s) | 755, 1030, 1080 |
| BstNSI | RCATGY | 2 cut(s) | 1878, 1909 |
| BstSCI | CCNGG | 4 cut(s) | 73, 753, 1028, 1078 |
| BstSFI | CTRYAG | 4 cut(s) | 171, 715, 1084, 1506 |
| BstV1I | GCAGC | 3 cut(s) | 452, 530, 608 |
| BstV2I | GAAGAC | 1 cut(s) | 2017 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1838 |
| BstZ17I | GTATAC | 2 cut(s) | 642, 1575 |
| Bsu36I | CCTNAGG | 1 cut(s) | 262 |
| BsuRI | GGCC | 4 cut(s) | 1417, 1630, 1675, 1720 |
| BtsCI | GGATG | 3 cut(s) | 750, 833, 1247 |
| BtsIMutI | CAGTG | 1 cut(s) | 1089 |
| Cac8I | GCNNGC | 2 cut(s) | 236, 1267 |
| CciI | TCATGA | 1 cut(s) | 2028 |
| CfoI | GCGC | 1 cut(s) | 1520 |
| Cfr13I | GGNCC | 1 cut(s) | 47 |
| CseI | GACGC | 1 cut(s) | 1574 |
| Csp6I | GTAC | 9 cut(s) | 477, 1407, 1530, 1620, 1665, 1710, 1796, 1903, 1990 |
| CspCI | CAANNNNNGTGG | 4 cut(s) | 7, 42, 989, 1024 |
| CviAII | CATG | 6 cut(s) | 183, 916, 1191, 1875, 1906, 2029 |
| CviQI | GTAC | 9 cut(s) | 477, 1407, 1530, 1620, 1665, 1710, 1796, 1903, 1990 |
| DpnI | GATC | 3 cut(s) | 90, 920, 1177 |
| DpnII | GATC | 3 cut(s) | 88, 918, 1175 |
| DraIII | CACNNNGTG | 1 cut(s) | 1135 |
| Eam1104I | CTCTTC | 2 cut(s) | 1476, 1809 |
| EarI | CTCTTC | 2 cut(s) | 1476, 1809 |
| Ecl136II | GAGCTC | 1 cut(s) | 886 |
| Eco147I | AGGCCT | 4 cut(s) | 1417, 1630, 1675, 1720 |
| Eco24I | GRGCYC | 2 cut(s) | 199, 888 |
| Eco31I | GGTCTC | 1 cut(s) | 1420 |
| Eco32I | GATATC | 1 cut(s) | 1026 |
| Eco47I | GGWCC | 1 cut(s) | 47 |
| Eco53kI | GAGCTC | 1 cut(s) | 886 |
| Eco57I | CTGAAG | 1 cut(s) | 1923 |
| Eco72I | CACGTG | 2 cut(s) | 413, 1752 |
| Eco81I | CCTNAGG | 1 cut(s) | 262 |
| Eco88I | CYCGRG | 1 cut(s) | 1762 |
| EcoICRI | GAGCTC | 1 cut(s) | 886 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1399 |
| EcoO109I | RGGNCCY | 1 cut(s) | 47 |
| EcoRII | CCWGG | 3 cut(s) | 753, 1028, 1078 |
| EcoRV | GATATC | 1 cut(s) | 1026 |
| EcoT22I | ATGCAT | 1 cut(s) | 309 |
| EcoT38I | GRGCYC | 2 cut(s) | 199, 888 |
| FaeI | CATG | 6 cut(s) | 186, 919, 1194, 1878, 1909, 2032 |
| FalI | AAGNNNNNCTT | 8 cut(s) | 110, 142, 136, 168, 1043, 1075, 1986, 2018 |
| FatI | CATG | 6 cut(s) | 182, 915, 1190, 1874, 1905, 2028 |
| FauI | CCCGC | 1 cut(s) | 667 |
| FbaI | TGATCA | 1 cut(s) | 918 |
| FblI | GTMKAC | 8 cut(s) | 418, 496, 574, 641, 1549, 1574, 1594, 1729 |
| Fnu4HI | GCNGC | 3 cut(s) | 441, 519, 597 |
| FokI | GGATG | 3 cut(s) | 737, 840, 1254 |
| FriOI | GRGCYC | 2 cut(s) | 199, 888 |
| Fsp4HI | GCNGC | 3 cut(s) | 441, 519, 597 |
| FspBI | CTAG | 5 cut(s) | 798, 822, 1056, 1113, 1401 |
| GlaI | GCGC | 1 cut(s) | 1519 |
| GluI | GCNGC | 3 cut(s) | 441, 519, 597 |
| GsuI | CTGGAG | 1 cut(s) | 872 |
| HaeIII | GGCC | 4 cut(s) | 1417, 1630, 1675, 1720 |
| HapII | CCGG | 2 cut(s) | 75, 1123 |
| HgaI | GACGC | 1 cut(s) | 1574 |
| HhaI | GCGC | 1 cut(s) | 1520 |
| Hin1I | GRCGYC | 1 cut(s) | 1585 |
| Hin1II | CATG | 6 cut(s) | 186, 919, 1194, 1878, 1909, 2032 |
| Hin6I | GCGC | 1 cut(s) | 1518 |
| HinP1I | GCGC | 1 cut(s) | 1518 |
| HindIII | AAGCTT | 1 cut(s) | 2000 |
| HpaII | CCGG | 2 cut(s) | 75, 1123 |
| Hpy188I | TCNGA | 8 cut(s) | 96, 162, 194, 274, 671, 1257, 1786, 1975 |
| HpyAV | CCTTC | 7 cut(s) | 37, 64, 204, 224, 363, 986, 1189 |
| HpyCH4III | ACNGT | 4 cut(s) | 476, 1848, 1917, 2023 |
| HpyCH4IV | ACGT | 2 cut(s) | 412, 1751 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 2085 |
| HpySE526I | ACGT | 2 cut(s) | 412, 1751 |
| Hsp92I | GRCGYC | 1 cut(s) | 1585 |
| Hsp92II | CATG | 6 cut(s) | 186, 919, 1194, 1878, 1909, 2032 |
| HspAI | GCGC | 1 cut(s) | 1518 |
| Kpn2I | TCCGGA | 1 cut(s) | 1122 |
| Ksp22I | TGATCA | 1 cut(s) | 918 |
| Kzo9I | GATC | 3 cut(s) | 88, 918, 1175 |
| LmnI | GCTCC | 3 cut(s) | 37, 361, 891 |
| Lsp1109I | GCAGC | 3 cut(s) | 452, 530, 608 |
| LweI | GCATC | 6 cut(s) | 382, 427, 505, 583, 733, 1252 |
| MaeI | CTAG | 5 cut(s) | 798, 822, 1056, 1113, 1401 |
| MaeII | ACGT | 2 cut(s) | 412, 1751 |
| MaeIII | GTNAC | 6 cut(s) | 470, 848, 1274, 1681, 1747, 2009 |
| MalI | GATC | 3 cut(s) | 90, 920, 1177 |
| MbiI | CCGCTC | 3 cut(s) | 432, 510, 588 |
| MboI | GATC | 3 cut(s) | 88, 918, 1175 |
| MfeI | CAATTG | 1 cut(s) | 2137 |
| MhlI | GDGCHC | 3 cut(s) | 199, 888, 1445 |
| MlyI | GAGTC | 2 cut(s) | 151, 1754 |
| Mph1103I | ATGCAT | 1 cut(s) | 309 |
| MroI | TCCGGA | 1 cut(s) | 1122 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1539 |
| MseI | TTAA | 3 cut(s) | 678, 1163, 1476 |
| MspA1I | CMGCKG | 4 cut(s) | 443, 521, 599, 1457 |
| MspI | CCGG | 2 cut(s) | 75, 1123 |
| MspR9I | CCNGG | 4 cut(s) | 75, 755, 1030, 1080 |
| MunI | CAATTG | 1 cut(s) | 2137 |
| Mva1269I | GAATGC | 1 cut(s) | 110 |
| MvaI | CCWGG | 3 cut(s) | 755, 1030, 1080 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 2085 |
| NciI | CCSGG | 1 cut(s) | 75 |
| NdeII | GATC | 3 cut(s) | 88, 918, 1175 |
| NlaIII | CATG | 6 cut(s) | 186, 919, 1194, 1878, 1909, 2032 |
| NlaIV | GGNNCC | 1 cut(s) | 48 |
| NmuCI | GTSAC | 2 cut(s) | 1274, 1747 |
| NsiI | ATGCAT | 1 cut(s) | 309 |
| NspI | RCATGY | 2 cut(s) | 1878, 1909 |
| PagI | TCATGA | 1 cut(s) | 2028 |
| PceI | AGGCCT | 4 cut(s) | 1417, 1630, 1675, 1720 |
| PctI | GAATGC | 1 cut(s) | 110 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1539 |
| PfoI | TCCNGGA | 2 cut(s) | 753, 1028 |
| PkrI | GCNGC | 3 cut(s) | 442, 520, 598 |
| PleI | GAGTC | 2 cut(s) | 151, 1754 |
| PmaCI | CACGTG | 2 cut(s) | 413, 1752 |
| PmlI | CACGTG | 2 cut(s) | 413, 1752 |
| PpsI | GAGTC | 2 cut(s) | 151, 1754 |
| Ppu21I | YACGTR | 2 cut(s) | 413, 1752 |
| PpuMI | RGGWCCY | 1 cut(s) | 47 |
| PshBI | ATTAAT | 1 cut(s) | 678 |
| Psp124BI | GAGCTC | 1 cut(s) | 888 |
| Psp5II | RGGWCCY | 1 cut(s) | 47 |
| Psp6I | CCWGG | 3 cut(s) | 753, 1028, 1078 |
| PspCI | CACGTG | 2 cut(s) | 413, 1752 |
| PspGI | CCWGG | 3 cut(s) | 753, 1028, 1078 |
| PspN4I | GGNNCC | 1 cut(s) | 48 |
| PspPI | GGNCC | 1 cut(s) | 47 |
| PspPPI | RGGWCCY | 1 cut(s) | 47 |
| PstI | CTGCAG | 1 cut(s) | 175 |
| PvuII | CAGCTG | 4 cut(s) | 443, 521, 599, 1457 |
| RsaI | GTAC | 9 cut(s) | 478, 1408, 1531, 1621, 1666, 1711, 1797, 1904, 1991 |
| RsaNI | GTAC | 9 cut(s) | 477, 1407, 1530, 1620, 1665, 1710, 1796, 1903, 1990 |
| SacI | GAGCTC | 1 cut(s) | 888 |
| SaqAI | TTAA | 3 cut(s) | 678, 1163, 1476 |
| SatI | GCNGC | 3 cut(s) | 441, 519, 597 |
| Sau3AI | GATC | 3 cut(s) | 88, 918, 1175 |
| Sau96I | GGNCC | 1 cut(s) | 47 |
| ScaI | AGTACT | 5 cut(s) | 478, 1408, 1531, 1621, 1711 |
| SchI | GAGTC | 2 cut(s) | 151, 1754 |
| ScrFI | CCNGG | 4 cut(s) | 75, 755, 1030, 1080 |
| SduI | GDGCHC | 3 cut(s) | 199, 888, 1445 |
| SfaNI | GCATC | 6 cut(s) | 382, 427, 505, 583, 733, 1252 |
| SfcI | CTRYAG | 4 cut(s) | 171, 715, 1084, 1506 |
| SinI | GGWCC | 1 cut(s) | 47 |
| SmlI | CTYRAG | 2 cut(s) | 224, 993 |
| SmoI | CTYRAG | 2 cut(s) | 224, 993 |
| SseBI | AGGCCT | 4 cut(s) | 1417, 1630, 1675, 1720 |
| SsiI | CCGC | 4 cut(s) | 432, 510, 588, 660 |
| SspMI | CTAG | 5 cut(s) | 798, 822, 1056, 1113, 1401 |
| SstI | GAGCTC | 1 cut(s) | 888 |
| StuI | AGGCCT | 4 cut(s) | 1417, 1630, 1675, 1720 |
| StyD4I | CCNGG | 4 cut(s) | 73, 753, 1028, 1078 |
| TaaI | ACNGT | 4 cut(s) | 476, 1848, 1917, 2023 |
| TaiI | ACGT | 2 cut(s) | 415, 1754 |
| TaqI | TCGA | 1 cut(s) | 1439 |
| TatI | WGTACW | 7 cut(s) | 476, 1406, 1529, 1619, 1709, 1795, 1902 |
| Tru1I | TTAA | 3 cut(s) | 678, 1163, 1476 |
| Tru9I | TTAA | 3 cut(s) | 678, 1163, 1476 |
| TscAI | CASTG | 1 cut(s) | 1096 |
| TseFI | GTSAC | 2 cut(s) | 1274, 1747 |
| TseI | GCWGC | 3 cut(s) | 440, 518, 596 |
| Tsp45I | GTSAC | 2 cut(s) | 1274, 1747 |
| TspDTI | ATGAA | 6 cut(s) | 147, 171, 739, 1285, 1965, 2017 |
| TspGWI | ACGGA | 1 cut(s) | 867 |
| TspRI | CASTG | 1 cut(s) | 1096 |
| VpaK11BI | GGWCC | 1 cut(s) | 47 |
| VspI | ATTAAT | 1 cut(s) | 678 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1399 |
| XapI | RAATTY | 5 cut(s) | 746, 940, 1780, 1787, 1969 |
| XbaI | TCTAGA | 1 cut(s) | 821 |
| XceI | RCATGY | 2 cut(s) | 1878, 1909 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1188 |
| XmiI | GTMKAC | 8 cut(s) | 418, 496, 574, 641, 1549, 1574, 1594, 1729 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1539 |
| XspI | CTAG | 5 cut(s) | 798, 822, 1056, 1113, 1401 |
| ZrmI | AGTACT | 5 cut(s) | 478, 1408, 1531, 1621, 1711 |
| Zsp2I | ATGCAT | 1 cut(s) | 309 |