Rorug04G0205300

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0000235
Physical Location & Seq
Forward (+)
35789704 .. 35794795
5092 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0205300.1

Sequence Viewer

Length: 3744 bp
ATGACAGAATTTGAAGCAGCTTCCTTCTCCTCTTCTTCTTCCAACCATCCATGGAAATACCATGTCTTTCTGAGTTTTAGAGGAGAGGATACACGCAACAACTTCACAGGCCATTTGTGCAGCGCGTTGCGTCAGAAGGGATTCAACACCTTCATGGATGATGAGCTCACAAGAGGAGAAGAAATATCAACAGCGCTTCTCCAAGCAATTGAACAGTCGAGGATTTCAATCGTTGTATTCTCTGAAAACTATGCATCCTCCAAGTGGTGCTTGGATGAACTCGACAAGATCCTCGAGTGCAAAGAATCAAAACAGCAACTGGTTCGACCAGTCTTTTACAAAGTGGATCCCTCGGATATACGAAACCATAGAGGAAGTTTTGGTGAGGCATTGGCTAATCTGGCACACCGATTCCAACATAACGTGGAAAAGGTTCAGAACTGGAAGGCGGCTCTTTCAAAAGCAGCAAATTTGTCTGGATGGCCTCTTTCAAAAGAGCAGTCTGAATCTAAACTTATTCATGACATCGTGGAAGAGATCTCAGAGCAAGTTATAAAGCATACGTATATGGATGTGGCAAAGTATCCGGTTGGGATAGAGACTCGTGTGCAAGATATAAATAAGTTGTTAGGTGTTCAGGGTAAAGATGTCCAAATGATAGGGATATGGGGGACTGGTGGAATAGGTAAGACAACTATTGCCAAAGCTGTCTATAATTCAAATGCCCACAAGTTTGAAGGTAGATGTTTCCTGGAAAGTGTGAGAGAAAATTCAATGTGCTATGGAGGCTTAGTCAGACAACAAGAGACCCTTCTTTTTGAGATTCTAGGGAGAAAAAAATTGAAGGTGACCAATGCTGCTATAGGAATCACTACCATAAAGGAAAGGTTGCGCTATAAAAGTGTTCTTCTAGTTCTTGATGATGTGAATAACCTGAATCAGTTAAACAACTTGGCTGGGGGAATTGATTGGTTTGGTAAGGGTAGTCGAATTCTCATAACCACAAGGGATAAAAGATTGCTGGTTGCTCATGACATTGACTTAATGTACAATGTCAAAGAATTAGATTATCATGAAGCTGTTGAGCTCTTCAGTTGGAATGCCTTTAAAAGAAAAGGACCTTTGGAGGTTTATGTGGAACTCACAGAACGTGCTATAGGCTATGCTCAAGGCCTTCCACTAGCTTTGAGAGTTCTAGGTTCTTATCTATATGGTAGAAACCTAGATACATGGAAAGCTACATTAGATGGTTTCAAAAGCCCTGAAATTCAAGAAATACTCAAAATTAGTTATGATGCATTGGTTGATTCGGTAAAGGAAGTGTTCCTAGATATCGCATGTTTCTTTAAAGGTGGAGATAGGAAATACGTGATAGAAATGCTAGAAGGTTGTGGCCGCAACTCTGACAATGGTATTAATGTGCTCATTGAAATGGCCCTCATCAATATTGATGAACACGATTGTATCCGTATGCATGACTTGCTAGAAGAAATGGGTAAAGACATAGTTCACCAAGAGTCACCCAATAATCCTGGAAAACGTAGCAGGCTGTGGTTTCATGATGATGTTTATAACGTTCTGACAGAAAATACAGGAACAACTAAGATTACAGGCATCAAGGTGGAGCTGCCCGAAGAATCAGATGAGATATGCTTAAGTATTACCACTTTCTCAAAGATGCGAAATCTTAGACTGTTTATACACCGAGCTGGATGCTTTTCTGGAGTTCTTGATTATCTGCCCAATAGCTTGAGGGTTGTAGATTGGCCTAACTGTCCCTTCCAATCTTTGCCATCTAATTTTAATCCAAGGAATCTTGCTGTACTCAAGATGCCTAGGAGTCGCATAACAGGACTGGTGGAGGGATTCAAGAATCTTACAAACCTGACATCCATAAATTTAAGTTTTTGTCAACATCTAACAAAAGTCTCCGACTTGTCCGAAATCCCAAACTTACAGATGTTGGATCTATCTTATAGTAGAAGCTTAGATGAAGTACATCCTTCTGTAGGATTTCTTGATAAACTTTCGACATTATATCTTGATGGATGCTCTAGCCTTATATCTTTTCCAACAAGAGTTAGATGGAGATCCATGACACATATTCGGCTTGATTACTGCACAAGGCTCGAGAATTTCGTAGAAATTGTGGAAAAGATGGATCGTCTATGGAGATTGAGTCTAAGTGGGACTGGCATAAAACAACTGCCTTCGTGGATTGGATATCTCACTTCCTTGAGAAAATTGAACATGTCTGGAACTCCCATTGAAGAATTGCCTTCATCTATTGGAAATCTCATTGAGCTTGAACAGTTAAATTTATATGAATGTGTAAACCTTAGAAATGTGCCGCAGAGTATTTACGGGTTGCAACATCTCTCGAATCTTAATCTCTGTGGGTGCCGAAATCTTGTTGTATTTCCAACAAAGGTGAGCTTTAAAGTTCCAGCAGTTGCAGAATCACTTCCTCTGGTGTTTCCAGCTAATTCAAACTTCTCACATAGCAACTGTGGTTCATTACAACTGTTTCCCCAGCTAATAAACCTATCATTTGAAGGATGCAATTTACTGTCAGTCTATGATTTCCTGGCTAGTCTTGATTGCTTGTTCACCTTAAAGAGACTCAATCTATCACGAAGCAATTTTGTTAGTCTTCCTATATGTATTAGCAAATTTCTCTGGTTGTGTCATCTTAATTTGCATGGTTGCAAGAGTCTTCGAGAAATCTTAGAACTTCCACCGTATATAGAAGTGATAGACATTGGCGGTTGTGTGTCACTGGAAAAGTTTCCAACATTGTCCAGGATATTGGAATTCAATGACACTCAATTCCTCAATTCGATAGACTTGTCTAATTGCCATAGGTTACTGGTTGATAATATTGGCTTCGACGTTGTGTCAAAGATGGCAAATGCATTAGTACTGAATCAGGTATATAAGAAATCTGAGTTTGAGGTCATACTTCCAGGAAGTGAAGTTCCAAAGTGGTTTGATCCGCGTAAGGTCGAAGTGTGTGTGACTATGCGTATTGACACTCAGGATGATATATGTACACTTATGATTGAAATCCCTAAGACTTTGAAATGGGAGAAAACAGGGCTTGTTGTTTGTGCTGTTTTTGAAGCTACGCAAGATTATTCTGGTGACTGCAGTTTTGGTCTCCACATTACAATCAATGAAGTAGAGATGGATGACTGCACTACATTAGGGTGTTACTTTGAAGCAACAGAAACAGAGCCATCAACTCATGTGTGGCTGAAATATATTCCAATGAATGTTGGCATGAAGGAAGAGGTAGATGCAAAGAGTGATCAACGGACACCTTATTCGTGTCAACTTAGCTTTTTCTATATAGGCGGTCAAGGGTTTCTCTTCAGAAGCTGTGGGGCCCACCTGGCTAGTGTGCCCTATGATGATGACGATGACGACGACCATGATTATGACGAGGACGAGGATGAGGACGAGGAGGAGGACGATGGTGATGAAGATGATGTAAGTAGTGAAGACTCAGCAGAAAAGAGAGATAGGAAGAGCACTGGTAGTATTTCTGAGTTGGGCTTGAGTTGTGATTTTGACAAAGCAGAAGCTGCCACTGTATCTACACCATCATCAGTATTATCAGGAGGCATTAGTACCAAGCGAGGTGTTGAACACTTTGATCATGATGGGGAACCTGGTCCAACAAAAAGATTTAAACAGATCACCCATGGAGTTGAACCTAATACCAGTGAAAGCGCTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0007275 GO:0008150 GO:0009553 GO:0009566 GO:0009567 GO:0019953 GO:0022414 GO:0032501 GO:0032502 GO:0044703 GO:0048229 GO:0048856 GO:0051704
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

1247

Amino Acids

140.8

Weight (kDa)

5.2

Isoelectric Point (pI)

44.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 19 - 184 3.4e-56 TIR domain
TIR_2 PF13676 22 - 117 9.4e-17 TIR domain
NB-ARC PF00931 202 - 371 7.6e-17 NB-ARC domain
WHD_ROQ1 PF23282 439 - 507 4.8e-18 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 714 - 799 1.2e-11 Leucine-rich repeat region
LRR_13 PF23286 748 - 945 1.8e-09 Disease resistance protein RPS4B-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000235)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g22700 FvH4_2g06920 FvH4_2g06920 FvH4_2g06960 FvH4_2g06960 FvH4_2g06960 FvH4_2g06960 FvH4_2g06970 FvH4_2g06970 FvH4_2g07230 FvH4_2g07231 FvH4_2g07231 FvH4_2g07240 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_4g19990 FvH4_4g19990 FvH4_4g19990 FvH4_4g20070
malus_domestica MD05G1072700.v1.1
prunus_persica Prupe.8G113100_v2.0.a1
pyrus_communis pycom05g06500 pycom10g06950
rosa_chinensis RchiOBHm_Chr4g0425711 RchiOBHm_Chr6g0259241 RchiOBHm_Chr6g0259251 RchiOBHm_Chr6g0259321 RchiOBHm_Chr6g0259331 RchiOBHm_Chr6g0259781 RchiOBHm_Chr6g0259791 RchiOBHm_Chr6g0259811 RchiOBHm_Chr6g0259881 RchiOBHm_Chr6g0259931
rosa_laevigata RLG00000007345 RLG00000014500 RLG00000014501 RLG00000014505 RLG00000014506 RLG00000014507 RLG00000014539 RLG00000014540 RLG00000014545 RLG00000014546
rosa_multiflora Rmu_co8137334.1_g000001 Rmu_co8454279.1_g000001 Rmu_co8476373.1_g000001 Rmu_sc0001078.1_g000001 Rmu_sc0003958.1_g000011 Rmu_sc0009049.1_g000001 Rmu_sc0009049.1_g000009 Rmu_sc0010546.1_g000001 Rmu_sc0011974.1_g000002 Rmu_sc0011974.1_g000003 Rmu_sc0033846.1_g000001 Rmu_sc0036999.1_g000001 Rmu_ssc0000052.1_g000015
rosa_roxburghii Rroxscaffold_5G00367690 Rroxscaffold_7G00206530 Rroxscaffold_7G00206540 Rroxscaffold_7G00206560 Rroxscaffold_7G00206570 Rroxscaffold_7G00207020 Rroxscaffold_7G00207030 Rroxscaffold_7G00207150 Rroxscaffold_7G00207160 Rroxscaffold_7G00207170 Rroxscaffold_7G00207270
rosa_rugosa Rorug04G0205300 Rorug05G0586300 Rorug05G0586400 Rorug05G0586500 Rorug05G0586600 Rorug05G0586700 Rorug05G0587300 Rorug05G0587400 Rorug05G0587500 Rorug05G0587600 Rorug05G0590500 Rorug05G0590600 Rorug05G0591200 Rorug05G0591300 Rorug05G0591400 Rorug05G0591500
rosa_samantha Rh4AG262200 Rh4BG268500 Rh4CG279700 Rh4DG264100 Rh6AG103100 Rh6AG103600 Rh6AG107100 Rh6AG107200 Rh6AG107700 Rh6BG095400 Rh6BG095500 Rh6BG095900 Rh6BG096000 Rh6BG096800 Rh6BG102600 Rh6BG102700 Rh6BG102800 Rh6BG103800 Rh6CG092300 Rh6CG092400 Rh6CG092800 Rh6CG092900 Rh6CG096200 Rh6CG096300 Rh6CG096800 Rh6CG097100 Rh6DG086300 Rh6DG086400 Rh6DG086700 Rh6DG086800 Rh6DG090000 Rh6DG090100 Rh6DG090500 Rh6DG090800
rosa_wichuraiana Rw4G022670 Rw6G008900 Rw6G008940 Rw6G009250 Rw6G009280 Rw6G009330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 554, 1572
AccB1I GGYRCC 1 cut(s) 2400
AccII CGCG 2 cut(s) 125, 2998
AciI CCGC 6 cut(s) 449, 1396, 2351, 2766, 2996, 3357
AclI AACGTT 1 cut(s) 1575
AclWI GGATC 7 cut(s) 283, 341, 354, 1974, 2085, 2169, 2987
AcoI YGGCCR 1 cut(s) 1393
AcuI CTGAAG 2 cut(s) 1075, 3358
AfaI GTAC 6 cut(s) 1049, 1824, 1998, 2922, 3052, 3634
AfeI AGCGCT 2 cut(s) 195, 3736
AfiI CCNNNNNNNGG 2 cut(s) 264, 2009
AflII CTTAAG 1 cut(s) 1654
AflIII ACRYGT 1 cut(s) 2250
AhdI GACNNNNNGTC 1 cut(s) 2896
AjnI CCWGG 7 cut(s) 750, 1531, 2586, 2801, 2965, 3393, 3673
AloI GAACNNNNNNTCC 2 cut(s) 2961, 2993
Alw21I GWGCWC 4 cut(s) 168, 1089, 1425, 3536
Alw26I GTCTC 5 cut(s) 593, 800, 1933, 2614, 3164
AlwI GGATC 7 cut(s) 283, 341, 354, 1974, 2085, 2169, 2987
AlwNI CAGNNNCTG 3 cut(s) 319, 3381, 3587
Ama87I CYCGRG 2 cut(s) 293, 2129
Aor51HI AGCGCT 2 cut(s) 195, 3736
AoxI GGCC 7 cut(s) 109, 482, 1171, 1393, 1434, 1766, 3387
ApaI GGGCCC 1 cut(s) 3391
ApeKI GCWGC 6 cut(s) 17, 120, 464, 857, 1627, 3587
ArsI GACNNNNNNTTYG 6 cut(s) 485, 517, 3137, 3169, 3310, 3342
AseI ATTAAT 1 cut(s) 1416
Asp700I GAANNNNTTC 4 cut(s) 140, 432, 2463, 2787
AspA2I CCTAGG 1 cut(s) 1835
AspLEI GCGC 4 cut(s) 125, 196, 894, 3737
AspS9I GGNCC 5 cut(s) 1118, 1435, 3387, 3388, 3677
AsuHPI GGTGA 9 cut(s) 395, 859, 1502, 1512, 2443, 2602, 3155, 3491, 3694
AvaI CYCGRG 2 cut(s) 293, 2129
AvaII GGWCC 2 cut(s) 1118, 3677
AvrII CCTAGG 1 cut(s) 1835
BaeGI GKGCMC 2 cut(s) 3391, 3408
BaeI ACNNNNGTAYC 2 cut(s) 1447, 1480
BamHI GGATCC 1 cut(s) 346
BanI GGYRCC 1 cut(s) 2400
BanII GRGCYC 3 cut(s) 168, 1089, 3391
BauI CACGAG 1 cut(s) 603
BbsI GAAGAC 3 cut(s) 2645, 2708, 3510
Bbv12I GWGCWC 4 cut(s) 168, 1089, 1425, 3536
BbvI GCAGC 6 cut(s) 29, 132, 476, 844, 1614, 3574
BcgI CGANNNNNNTGC 6 cut(s) 305, 339, 1695, 1729, 2110, 2144
BciT130I CCWGG 7 cut(s) 752, 1533, 2588, 2803, 2967, 3395, 3675
BciVI GTATCC 3 cut(s) 82, 594, 1475
BclI TGATCA 2 cut(s) 3310, 3658
BcoDI GTCTC 5 cut(s) 593, 800, 1933, 2614, 3164
BfmI CTRYAG 4 cut(s) 861, 1155, 2007, 3148
BfoI RGCGCY 2 cut(s) 197, 3738
BfrI CTTAAG 1 cut(s) 1654
BfuI GTATCC 3 cut(s) 82, 594, 1475
BglI GCCNNNNNGGC 1 cut(s) 3395
BglII AGATCT 1 cut(s) 537
BisI GCNGC 9 cut(s) 18, 121, 450, 465, 858, 1396, 1628, 2351, 3588
BlnI CCTAGG 1 cut(s) 1835
BlsI GCNGC 9 cut(s) 19, 122, 451, 466, 859, 1397, 1629, 2352, 3589
BmcAI AGTACT 1 cut(s) 2922
Bme1390I CCNGG 7 cut(s) 752, 1533, 2588, 2803, 2967, 3395, 3675
Bme18I GGWCC 2 cut(s) 1118, 3677
BmeRI GACNNNNNGTC 1 cut(s) 2896
BmeT110I CYCGRG 2 cut(s) 293, 2129
BmgT120I GGNCC 5 cut(s) 1118, 1435, 3387, 3388, 3677
BmiI GGNNCC 5 cut(s) 348, 2402, 3388, 3389, 3672
BmrFI CCNGG 7 cut(s) 752, 1533, 2588, 2803, 2967, 3395, 3675
BmsI GCATC 9 cut(s) 263, 1285, 1623, 1668, 1703, 1821, 2039, 2549, 3289
BpiI GAAGAC 3 cut(s) 2645, 2708, 3510
BpmI CTGGAG 1 cut(s) 1743
BpuEI CTTGAG 5 cut(s) 1152, 1771, 1811, 2257, 3580
BsaAI YACGTR 2 cut(s) 564, 1369
BsaBI GATNNNNATC 3 cut(s) 227, 2089, 3065
BsaI GGTCTC 2 cut(s) 800, 3164
BsaJI CCNNGG 5 cut(s) 50, 351, 1808, 1835, 3706
BsaWI WCCGGW 1 cut(s) 586
BsaXI ACNNNNNCTCC 4 cut(s) 777, 807, 2164, 2194
Bsc4I CCNNNNNNNGG 2 cut(s) 264, 2009
Bse8I GATNNNNATC 3 cut(s) 227, 2089, 3065
BseBI CCWGG 7 cut(s) 752, 1533, 2588, 2803, 2967, 3395, 3675
BseDI CCNNGG 5 cut(s) 50, 351, 1808, 1835, 3706
BseJI GATNNNNATC 3 cut(s) 227, 2089, 3065
BseLI CCNNNNNNNGG 2 cut(s) 264, 2009
BseMII CTCAG 6 cut(s) 62, 555, 2937, 3050, 3522, 3540
BseRI GAGGAG 5 cut(s) 19, 96, 189, 3479, 3482
BseSI GKGCMC 2 cut(s) 3391, 3408
BseXI GCAGC 6 cut(s) 29, 132, 476, 844, 1614, 3574
BseYI CCCAGC 2 cut(s) 956, 2532
BsgI GTGCAG 3 cut(s) 139, 2104, 3181
Bsh1236I CGCG 2 cut(s) 125, 2998
BshFI GGCC 7 cut(s) 111, 484, 1173, 1395, 1436, 1768, 3389
BshNI GGYRCC 1 cut(s) 2400
BsiHKAI GWGCWC 4 cut(s) 168, 1089, 1425, 3536
BsiHKCI CYCGRG 2 cut(s) 293, 2129
BsiSI CCGG 1 cut(s) 587
BslFI GGGAC 3 cut(s) 685, 1761, 2203
BslI CCNNNNNNNGG 2 cut(s) 264, 2009
BsmAI GTCTC 5 cut(s) 593, 800, 1933, 2614, 3164
BsmFI GGGAC 3 cut(s) 685, 1761, 2203
BsmI GAATGC 1 cut(s) 1105
BsnI GGCC 7 cut(s) 111, 484, 1173, 1395, 1436, 1768, 3389
Bso31I GGTCTC 2 cut(s) 800, 3164
BsoBI CYCGRG 2 cut(s) 293, 2129
Bsp120I GGGCCC 1 cut(s) 3387
Bsp1286I GDGCHC 6 cut(s) 168, 1089, 1425, 3391, 3408, 3536
Bsp1407I TGTACA 2 cut(s) 1047, 3050
Bsp19I CCATGG 2 cut(s) 50, 3706
BspACI CCGC 6 cut(s) 449, 1396, 2351, 2766, 2996, 3357
BspANI GGCC 7 cut(s) 111, 484, 1173, 1395, 1436, 1768, 3389
BspCNI CTCAG 6 cut(s) 63, 554, 2938, 3049, 3521, 3541
BspFNI CGCG 2 cut(s) 125, 2998
BspHI TCATGA 5 cut(s) 520, 1030, 1072, 1558, 3661
BspLI GGNNCC 5 cut(s) 348, 2402, 3388, 3389, 3672
BspMAI CTGCAG 1 cut(s) 3152
BspPI GGATC 7 cut(s) 283, 341, 354, 1974, 2085, 2169, 2987
BspQI GCTCTTC 2 cut(s) 1094, 3524
BspT107I GGYRCC 1 cut(s) 2400
BspTI CTTAAG 1 cut(s) 1654
BspTNI GGTCTC 2 cut(s) 800, 3164
BsrGI TGTACA 2 cut(s) 1047, 3050
BssECI CCNNGG 5 cut(s) 50, 351, 1808, 1835, 3706
BssSI CACGAG 1 cut(s) 603
BssT1I CCWWGG 4 cut(s) 50, 1808, 1835, 3706
Bst2BI CACGAG 1 cut(s) 603
Bst2UI CCWGG 7 cut(s) 752, 1533, 2588, 2803, 2967, 3395, 3675
Bst4CI ACNGT 9 cut(s) 216, 1695, 1775, 2313, 2510, 2526, 2571, 2742, 3595
Bst6I CTCTTC 6 cut(s) 37, 528, 1094, 3285, 3377, 3524
BstAFI CTTAAG 1 cut(s) 1654
BstAPI GCANNNNNTGC 2 cut(s) 1480, 3587
BstAUI TGTACA 2 cut(s) 1047, 3050
BstBAI YACGTR 2 cut(s) 564, 1369
BstC8I GCNNGC 1 cut(s) 1547
BstDSI CCRYGG 2 cut(s) 50, 3706
BstEII GGTNACC 1 cut(s) 847
BstENI CCTNNNNNAGG 1 cut(s) 2007
BstFNI CGCG 2 cut(s) 125, 2998
BstH2I RGCGCY 2 cut(s) 197, 3738
BstHHI GCGC 4 cut(s) 125, 196, 894, 3737
BstMAI GTCTC 5 cut(s) 593, 800, 1933, 2614, 3164
BstMWI GCNNNNNNNGC 6 cut(s) 117, 401, 786, 1480, 3395, 3587
BstNI CCWGG 7 cut(s) 752, 1533, 2588, 2803, 2967, 3395, 3675
BstNSI RCATGY 2 cut(s) 1341, 2254
BstPI GGTNACC 1 cut(s) 847
BstSCI CCNGG 7 cut(s) 750, 1531, 2586, 2801, 2965, 3393, 3673
BstSFI CTRYAG 4 cut(s) 861, 1155, 2007, 3148
BstSLI GKGCMC 2 cut(s) 3391, 3408
BstSNI TACGTA 1 cut(s) 564
BstUI CGCG 2 cut(s) 125, 2998
BstV1I GCAGC 6 cut(s) 29, 132, 476, 844, 1614, 3574
BstV2I GAAGAC 3 cut(s) 2645, 2708, 3510
BstX2I RGATCY 5 cut(s) 288, 346, 537, 1966, 2090
BstXI CCANNNNNNTGG 1 cut(s) 2809
BstYI RGATCY 5 cut(s) 288, 346, 537, 1966, 2090
BsuI GTATCC 3 cut(s) 82, 594, 1475
BsuRI GGCC 7 cut(s) 111, 484, 1173, 1395, 1436, 1768, 3389
BtgI CCRYGG 2 cut(s) 50, 3706
BtsIMutI CAGTG 4 cut(s) 2777, 3534, 3591, 3733
Cac8I GCNNGC 1 cut(s) 1547
CaiI CAGNNNCTG 3 cut(s) 319, 3381, 3587
CciI TCATGA 5 cut(s) 520, 1030, 1072, 1558, 3661
CfoI GCGC 4 cut(s) 125, 196, 894, 3737
Cfr13I GGNCC 5 cut(s) 1118, 1435, 3387, 3388, 3677
CseI GACGC 1 cut(s) 119
CsiI ACCWGGT 1 cut(s) 3673
Csp6I GTAC 6 cut(s) 1048, 1823, 1997, 2921, 3051, 3633
CspCI CAANNNNNGTGG 2 cut(s) 1167, 1202
CviQI GTAC 6 cut(s) 1048, 1823, 1997, 2921, 3051, 3633
DraI TTTAAA 4 cut(s) 1108, 1348, 2440, 3694
DriI GACNNNNNGTC 1 cut(s) 2896
EaeI YGGCCR 1 cut(s) 1393
Eam1104I CTCTTC 6 cut(s) 37, 528, 1094, 3285, 3377, 3524
Eam1105I GACNNNNNGTC 1 cut(s) 2896
EarI CTCTTC 6 cut(s) 37, 528, 1094, 3285, 3377, 3524
Ecl136II GAGCTC 2 cut(s) 166, 1087
Eco105I TACGTA 1 cut(s) 564
Eco130I CCWWGG 4 cut(s) 50, 1808, 1835, 3706
Eco147I AGGCCT 1 cut(s) 1173
Eco24I GRGCYC 3 cut(s) 168, 1089, 3391
Eco31I GGTCTC 2 cut(s) 800, 3164
Eco32I GATATC 2 cut(s) 1333, 2225
Eco47I GGWCC 2 cut(s) 1118, 3677
Eco47III AGCGCT 2 cut(s) 195, 3736
Eco53kI GAGCTC 2 cut(s) 166, 1087
Eco57I CTGAAG 2 cut(s) 1075, 3358
Eco88I CYCGRG 2 cut(s) 293, 2129
Eco91I GGTNACC 1 cut(s) 847
EcoICRI GAGCTC 2 cut(s) 166, 1087
EcoNI CCTNNNNNAGG 1 cut(s) 2007
EcoO109I RGGNCCY 2 cut(s) 1118, 3387
EcoO65I GGTNACC 1 cut(s) 847
EcoRI GAATTC 2 cut(s) 990, 2813
EcoRII CCWGG 7 cut(s) 750, 1531, 2586, 2801, 2965, 3393, 3673
EcoRV GATATC 2 cut(s) 1333, 2225
EcoT14I CCWWGG 4 cut(s) 50, 1808, 1835, 3706
EcoT22I ATGCAT 4 cut(s) 256, 1300, 1476, 2917
EcoT38I GRGCYC 3 cut(s) 168, 1089, 3391
ErhI CCWWGG 4 cut(s) 50, 1808, 1835, 3706
FalI AAGNNNNNCTT 6 cut(s) 254, 286, 795, 827, 2420, 2452
FaqI GGGAC 3 cut(s) 685, 1761, 2203
FbaI TGATCA 2 cut(s) 3310, 3658
Fnu4HI GCNGC 9 cut(s) 18, 121, 450, 465, 858, 1396, 1628, 2351, 3588
FriOI GRGCYC 3 cut(s) 168, 1089, 3391
Fsp4HI GCNGC 9 cut(s) 18, 121, 450, 465, 858, 1396, 1628, 2351, 3588
GlaI GCGC 4 cut(s) 124, 195, 893, 3736
GluI GCNGC 9 cut(s) 18, 121, 450, 465, 858, 1396, 1628, 2351, 3588
GsaI CCCAGC 2 cut(s) 960, 2536
GsuI CTGGAG 1 cut(s) 1743
HaeII RGCGCY 2 cut(s) 197, 3738
HaeIII GGCC 7 cut(s) 111, 484, 1173, 1395, 1436, 1768, 3389
HapII CCGG 1 cut(s) 587
HgaI GACGC 1 cut(s) 119
HhaI GCGC 4 cut(s) 125, 196, 894, 3737
Hin6I GCGC 4 cut(s) 123, 194, 892, 3735
HinP1I GCGC 4 cut(s) 123, 194, 892, 3735
HincII GTYRAC 2 cut(s) 1913, 3335
HindII GTYRAC 2 cut(s) 1913, 3335
HindIII AAGCTT 1 cut(s) 1984
HpaII CCGG 1 cut(s) 587
HphI GGTGA 9 cut(s) 395, 859, 1502, 1512, 2443, 2602, 3155, 3491, 3694
Hpy166II GTNNAC 6 cut(s) 1510, 1913, 2335, 2610, 3053, 3335
Hpy8I GTNNAC 6 cut(s) 1510, 1913, 2335, 2610, 3053, 3335
Hpy99I CGWCG 2 cut(s) 2894, 3431
HpyCH4III ACNGT 9 cut(s) 216, 1695, 1775, 2313, 2510, 2526, 2571, 2742, 3595
HpyCH4IV ACGT 7 cut(s) 423, 563, 1150, 1368, 1540, 1575, 2892
HpyF10VI GCNNNNNNNGC 6 cut(s) 117, 401, 786, 1480, 3395, 3587
HpySE526I ACGT 7 cut(s) 423, 563, 1150, 1368, 1540, 1575, 2892
HspAI GCGC 4 cut(s) 123, 194, 892, 3735
Ksp22I TGATCA 2 cut(s) 3310, 3658
LguI GCTCTTC 2 cut(s) 1094, 3524
LmnI GCTCC 1 cut(s) 1624
Lsp1109I GCAGC 6 cut(s) 29, 132, 476, 844, 1614, 3574
LweI GCATC 9 cut(s) 263, 1285, 1623, 1668, 1703, 1821, 2039, 2549, 3289
MabI ACCWGGT 1 cut(s) 3673
MaeII ACGT 7 cut(s) 423, 563, 1150, 1368, 1540, 1575, 2892
MaeIII GTNAC 7 cut(s) 847, 1518, 2775, 2865, 3016, 3143, 3212
MfeI CAATTG 1 cut(s) 207
MflI RGATCY 5 cut(s) 288, 346, 537, 1966, 2090
MhlI GDGCHC 6 cut(s) 168, 1089, 1425, 3391, 3408, 3536
MlyI GAGTC 7 cut(s) 595, 1526, 1849, 2188, 2616, 2722, 3500
MmeI TCCRAC 9 cut(s) 66, 439, 1076, 1944, 1956, 2096, 2447, 2816, 3704
Mph1103I ATGCAT 4 cut(s) 256, 1300, 1476, 2917
MroXI GAANNNNTTC 4 cut(s) 140, 432, 2463, 2787
MslI CAYNNNNRTG 6 cut(s) 152, 1430, 1563, 1619, 3208, 3438
MspCI CTTAAG 1 cut(s) 1654
MspI CCGG 1 cut(s) 587
MspR9I CCNGG 7 cut(s) 752, 1533, 2588, 2803, 2967, 3395, 3675
MunI CAATTG 1 cut(s) 207
Mva1269I GAATGC 1 cut(s) 1105
MvaI CCWGG 7 cut(s) 752, 1533, 2588, 2803, 2967, 3395, 3675
MvnI CGCG 2 cut(s) 125, 2998
MwoI GCNNNNNNNGC 6 cut(s) 117, 401, 786, 1480, 3395, 3587
NcoI CCATGG 2 cut(s) 50, 3706
NlaIV GGNNCC 5 cut(s) 348, 2402, 3388, 3389, 3672
NmuCI GTSAC 5 cut(s) 847, 1518, 2775, 3016, 3143
NsiI ATGCAT 4 cut(s) 256, 1300, 1476, 2917
NspI RCATGY 2 cut(s) 1341, 2254
PaeR7I CTCGAG 2 cut(s) 293, 2129
PagI TCATGA 5 cut(s) 520, 1030, 1072, 1558, 3661
PceI AGGCCT 1 cut(s) 1173
PciI ACATGT 1 cut(s) 2250
PciSI GCTCTTC 2 cut(s) 1094, 3524
PcsI WCGNNNNNNNCGW 2 cut(s) 2136, 3426
PctI GAATGC 1 cut(s) 1105
PdmI GAANNNNTTC 4 cut(s) 140, 432, 2463, 2787
PfoI TCCNGGA 4 cut(s) 750, 1531, 2801, 2965
PkrI GCNGC 9 cut(s) 19, 122, 451, 466, 859, 1397, 1629, 2352, 3589
PleI GAGTC 7 cut(s) 595, 1525, 1848, 2187, 2616, 2721, 3500
PpsI GAGTC 7 cut(s) 595, 1525, 1848, 2187, 2616, 2721, 3500
Ppu21I YACGTR 2 cut(s) 564, 1369
PpuMI RGGWCCY 1 cut(s) 1118
PscI ACATGT 1 cut(s) 2250
PshBI ATTAAT 1 cut(s) 1416
PsiI TTATAA 2 cut(s) 554, 1572
Psp124BI GAGCTC 2 cut(s) 168, 1089
Psp1406I AACGTT 1 cut(s) 1575
Psp5II RGGWCCY 1 cut(s) 1118
Psp6I CCWGG 7 cut(s) 750, 1531, 2586, 2801, 2965, 3393, 3673
PspEI GGTNACC 1 cut(s) 847
PspFI CCCAGC 2 cut(s) 956, 2532
PspGI CCWGG 7 cut(s) 750, 1531, 2586, 2801, 2965, 3393, 3673
PspN4I GGNNCC 5 cut(s) 348, 2402, 3388, 3389, 3672
PspOMI GGGCCC 1 cut(s) 3387
PspPI GGNCC 5 cut(s) 1118, 1435, 3387, 3388, 3677
PspPPI RGGWCCY 1 cut(s) 1118
PspXI VCTCGAGB 1 cut(s) 293
PstI CTGCAG 1 cut(s) 3152
PstNI CAGNNNCTG 3 cut(s) 319, 3381, 3587
PsuI RGATCY 5 cut(s) 288, 346, 537, 1966, 2090
RsaI GTAC 6 cut(s) 1049, 1824, 1998, 2922, 3052, 3634
RsaNI GTAC 6 cut(s) 1048, 1823, 1997, 2921, 3051, 3633
RseI CAYNNNNRTG 6 cut(s) 152, 1430, 1563, 1619, 3208, 3438
SacI GAGCTC 2 cut(s) 168, 1089
SapI GCTCTTC 2 cut(s) 1094, 3524
SatI GCNGC 9 cut(s) 18, 121, 450, 465, 858, 1396, 1628, 2351, 3588
Sau96I GGNCC 5 cut(s) 1118, 1435, 3387, 3388, 3677
ScaI AGTACT 1 cut(s) 2922
SchI GAGTC 7 cut(s) 595, 1526, 1849, 2188, 2616, 2722, 3500
ScrFI CCNGG 7 cut(s) 752, 1533, 2588, 2803, 2967, 3395, 3675
SduI GDGCHC 6 cut(s) 168, 1089, 1425, 3391, 3408, 3536
SexAI ACCWGGT 1 cut(s) 3673
SfaNI GCATC 9 cut(s) 263, 1285, 1623, 1668, 1703, 1821, 2039, 2549, 3289
SfcI CTRYAG 4 cut(s) 861, 1155, 2007, 3148
Sfr274I CTCGAG 2 cut(s) 293, 2129
SinI GGWCC 2 cut(s) 1118, 3677
SlaI CTCGAG 2 cut(s) 293, 2129
SmiMI CAYNNNNRTG 6 cut(s) 152, 1430, 1563, 1619, 3208, 3438
SmlI CTYRAG 8 cut(s) 293, 1167, 1654, 1750, 1826, 2129, 2236, 3559
SmoI CTYRAG 8 cut(s) 293, 1167, 1654, 1750, 1826, 2129, 2236, 3559
SnaBI TACGTA 1 cut(s) 564
SseBI AGGCCT 1 cut(s) 1173
SsiI CCGC 6 cut(s) 449, 1396, 2351, 2766, 2996, 3357
SspI AATATT 2 cut(s) 1447, 2881
SstI GAGCTC 2 cut(s) 168, 1089
StuI AGGCCT 1 cut(s) 1173
StyD4I CCNGG 7 cut(s) 750, 1531, 2586, 2801, 2965, 3393, 3673
StyI CCWWGG 4 cut(s) 50, 1808, 1835, 3706
TaaI ACNGT 9 cut(s) 216, 1695, 1775, 2313, 2510, 2526, 2571, 2742, 3595
TaiI ACGT 7 cut(s) 426, 566, 1153, 1371, 1543, 1578, 2895
TatI WGTACW 5 cut(s) 1047, 1822, 1996, 2920, 3050
TauI GCSGC 3 cut(s) 452, 1398, 2353
TscAI CASTG 4 cut(s) 2784, 3541, 3598, 3733
TseFI GTSAC 5 cut(s) 847, 1518, 2775, 3016, 3143
TseI GCWGC 6 cut(s) 17, 120, 464, 857, 1627, 3587
Tsp45I GTSAC 5 cut(s) 847, 1518, 2775, 3016, 3143
TspGWI ACGGA 2 cut(s) 1457, 3331
TspRI CASTG 4 cut(s) 2784, 3541, 3598, 3733
Vha464I CTTAAG 1 cut(s) 1654
VpaK11BI GGWCC 2 cut(s) 1118, 3677
VspI ATTAAT 1 cut(s) 1416
XagI CCTNNNNNAGG 1 cut(s) 2007
XceI RCATGY 2 cut(s) 1341, 2254
XcmI CCANNNNNNNNNTGG 1 cut(s) 268
XhoI CTCGAG 2 cut(s) 293, 2129
XmaJI CCTAGG 1 cut(s) 1835
XmnI GAANNNNTTC 4 cut(s) 140, 432, 2463, 2787
ZrmI AGTACT 1 cut(s) 2922
Zsp2I ATGCAT 4 cut(s) 256, 1300, 1476, 2917
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.