Rh6DG086300

B3 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0000235
Physical Location & Seq
Reverse (-)
10084324 .. 10086694
2371 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG086300.1

Sequence Viewer

Length: 735 bp
ATGGTGAGAAAGAATGGTGTCAAGGAAAAGAGGCCTTCATTCTTCAAGGTTATTCTTCCAGGTTACTCCACTGAACGTCTCATGATTCCAGCGCAGTTCTTGAAGCATCTTAGGAAGGATCTATGTAGCAAGGCAACTTTGAGCTTGAACATGTCTTCACAGTGTTCATGGAATGTGAAAGTGACTAAAACAAAGAAAGATGTGTATTTCAAGGATGGGTGGCAGGAGTTTTTAAACAATAACTCAGTGGATGAAGGCGACTTCCTAGTGTTCACCTATGAAGGGAAAATGCATTTCAGCATAGCGGTTTTCGACAGGAGTTGTTGTGGAAGAGTTGATTTTCCTAATGAAACATTCAAAGAATCCATAGTTGCTATGACTACTAAAAGGCCTTGTGGTAGACCAAGAAAAGATAACTCAGCTGCAGTTAACACAGCTAAGGAATCTGCTATATCTTTCAAATCCAAGTTTCCTCATTACAAAAGTACTATTAAGAAAACTTATTGTGTGTACTCAATTCCCACCACCTTCTACAGGGAGCATCTTTCTGCAGGCAAATATGAATTTGCTTTCCTGAAGATCTCAGGGGGAAACAAATGGTACAAAGTGAAGCTTGTCCCTTCCATTCGAACAGCCTTCATGTCCAAAGGGTGGGCTGCGTTTGCGCGTGATAACCAGATCAAGCATCTGATGAGAGCTTCAAGCTGGAATACTGAAAATCGTGGCTACCGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0007275 GO:0008150 GO:0009553 GO:0009566 GO:0009567 GO:0019953 GO:0022414 GO:0032501 GO:0032502 GO:0044703 GO:0048229 GO:0048856 GO:0051704
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3

Protein Analysis

244

Amino Acids

28.2

Weight (kDa)

9.91

Isoelectric Point (pI)

27.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B3 PF02362 14 - 106 1.7e-16 B3 DNA binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000235)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g22700 FvH4_2g06920 FvH4_2g06920 FvH4_2g06960 FvH4_2g06960 FvH4_2g06960 FvH4_2g06960 FvH4_2g06970 FvH4_2g06970 FvH4_2g07230 FvH4_2g07231 FvH4_2g07231 FvH4_2g07240 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07290 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_2g07300 FvH4_4g19990 FvH4_4g19990 FvH4_4g19990 FvH4_4g20070
malus_domestica MD05G1072700.v1.1
prunus_persica Prupe.8G113100_v2.0.a1
pyrus_communis pycom05g06500 pycom10g06950
rosa_chinensis RchiOBHm_Chr4g0425711 RchiOBHm_Chr6g0259241 RchiOBHm_Chr6g0259251 RchiOBHm_Chr6g0259321 RchiOBHm_Chr6g0259331 RchiOBHm_Chr6g0259781 RchiOBHm_Chr6g0259791 RchiOBHm_Chr6g0259811 RchiOBHm_Chr6g0259881 RchiOBHm_Chr6g0259931
rosa_laevigata RLG00000007345 RLG00000014500 RLG00000014501 RLG00000014505 RLG00000014506 RLG00000014507 RLG00000014539 RLG00000014540 RLG00000014545 RLG00000014546
rosa_multiflora Rmu_co8137334.1_g000001 Rmu_co8454279.1_g000001 Rmu_co8476373.1_g000001 Rmu_sc0001078.1_g000001 Rmu_sc0003958.1_g000011 Rmu_sc0009049.1_g000001 Rmu_sc0009049.1_g000009 Rmu_sc0010546.1_g000001 Rmu_sc0011974.1_g000002 Rmu_sc0011974.1_g000003 Rmu_sc0033846.1_g000001 Rmu_sc0036999.1_g000001 Rmu_ssc0000052.1_g000015
rosa_roxburghii Rroxscaffold_5G00367690 Rroxscaffold_7G00206530 Rroxscaffold_7G00206540 Rroxscaffold_7G00206560 Rroxscaffold_7G00206570 Rroxscaffold_7G00207020 Rroxscaffold_7G00207030 Rroxscaffold_7G00207150 Rroxscaffold_7G00207160 Rroxscaffold_7G00207170 Rroxscaffold_7G00207270
rosa_rugosa Rorug04G0205300 Rorug05G0586300 Rorug05G0586400 Rorug05G0586500 Rorug05G0586600 Rorug05G0586700 Rorug05G0587300 Rorug05G0587400 Rorug05G0587500 Rorug05G0587600 Rorug05G0590500 Rorug05G0590600 Rorug05G0591200 Rorug05G0591300 Rorug05G0591400 Rorug05G0591500
rosa_samantha Rh4AG262200 Rh4BG268500 Rh4CG279700 Rh4DG264100 Rh6AG103100 Rh6AG103600 Rh6AG107100 Rh6AG107200 Rh6AG107700 Rh6BG095400 Rh6BG095500 Rh6BG095900 Rh6BG096000 Rh6BG096800 Rh6BG102600 Rh6BG102700 Rh6BG102800 Rh6BG103800 Rh6CG092300 Rh6CG092400 Rh6CG092800 Rh6CG092900 Rh6CG096200 Rh6CG096300 Rh6CG096800 Rh6CG097100 Rh6DG086300 Rh6DG086400 Rh6DG086700 Rh6DG086800 Rh6DG090000 Rh6DG090100 Rh6DG090500 Rh6DG090800
rosa_wichuraiana Rw4G022670 Rw6G008900 Rw6G008940 Rw6G009250 Rw6G009280 Rw6G009330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 651
AccI GTMKAC 1 cut(s) 400
AccII CGCG 1 cut(s) 667
AciI CCGC 2 cut(s) 305, 730
AclWI GGATC 1 cut(s) 126
AcsI RAATTY 1 cut(s) 563
AcuI CTGAAG 1 cut(s) 596
AfaI GTAC 3 cut(s) 487, 512, 602
AfiI CCNNNNNNNGG 3 cut(s) 282, 534, 651
AflIII ACRYGT 1 cut(s) 150
AgsI TTSAA 7 cut(s) 46, 103, 148, 211, 358, 460, 702
AjnI CCWGG 1 cut(s) 58
AluBI AGCT 6 cut(s) 144, 422, 437, 613, 698, 705
AluI AGCT 6 cut(s) 144, 422, 437, 613, 698, 705
Alw26I GTCTC 1 cut(s) 83
AlwI GGATC 1 cut(s) 126
AoxI GGCC 2 cut(s) 32, 389
ApeKI GCWGC 2 cut(s) 422, 656
ApoI RAATTY 1 cut(s) 563
AspLEI GCGC 2 cut(s) 94, 667
AsuHPI GGTGA 2 cut(s) 16, 265
AsuII TTCGAA 1 cut(s) 628
BbsI GAAGAC 1 cut(s) 147
BbvI GCAGC 2 cut(s) 409, 643
BccI CCATC 1 cut(s) 209
BciT130I CCWGG 1 cut(s) 60
BcoDI GTCTC 1 cut(s) 83
BfaI CTAG 2 cut(s) 266, 733
BfmI CTRYAG 3 cut(s) 423, 532, 549
BglII AGATCT 1 cut(s) 579
BisI GCNGC 2 cut(s) 423, 657
BlsI GCNGC 2 cut(s) 424, 658
BmcAI AGTACT 1 cut(s) 487
Bme1390I CCNGG 1 cut(s) 60
BmrFI CCNGG 1 cut(s) 60
BmsI GCATC 3 cut(s) 115, 550, 694
BpiI GAAGAC 1 cut(s) 147
Bpu10I CCTNAGC 1 cut(s) 438
Bpu14I TTCGAA 1 cut(s) 628
BsaXI ACNNNNNCTCC 2 cut(s) 310, 340
Bsc4I CCNNNNNNNGG 3 cut(s) 282, 534, 651
BseBI CCWGG 1 cut(s) 60
BseGI GGATG 2 cut(s) 220, 256
BseLI CCNNNNNNNGG 3 cut(s) 282, 534, 651
BseMII CTCAG 3 cut(s) 258, 432, 597
BseXI GCAGC 2 cut(s) 409, 643
Bsh1236I CGCG 1 cut(s) 667
BshFI GGCC 2 cut(s) 34, 391
BslFI GGGAC 1 cut(s) 602
BslI CCNNNNNNNGG 3 cut(s) 282, 534, 651
BsmAI GTCTC 1 cut(s) 83
BsmBI CGTCTC 1 cut(s) 83
BsmFI GGGAC 1 cut(s) 602
BsnI GGCC 2 cut(s) 34, 391
Bsp119I TTCGAA 1 cut(s) 628
Bsp143I GATC 3 cut(s) 118, 579, 678
BspACI CCGC 2 cut(s) 305, 730
BspANI GGCC 2 cut(s) 34, 391
BspCNI CTCAG 3 cut(s) 257, 431, 596
BspFNI CGCG 1 cut(s) 667
BspHI TCATGA 1 cut(s) 81
BspMAI CTGCAG 2 cut(s) 427, 553
BspPI GGATC 1 cut(s) 126
BspT104I TTCGAA 1 cut(s) 628
BssMI GATC 3 cut(s) 118, 579, 678
Bst2UI CCWGG 1 cut(s) 60
Bst4CI ACNGT 1 cut(s) 162
Bst6I CTCTTC 1 cut(s) 325
BstBI TTCGAA 1 cut(s) 628
BstC8I GCNNGC 1 cut(s) 553
BstDEI CTNAG 5 cut(s) 110, 244, 418, 438, 583
BstENI CCTNNNNNAGG 1 cut(s) 532
BstF5I GGATG 2 cut(s) 220, 256
BstFNI CGCG 1 cut(s) 667
BstHHI GCGC 2 cut(s) 94, 667
BstKTI GATC 3 cut(s) 121, 582, 681
BstMAI GTCTC 1 cut(s) 83
BstMBI GATC 3 cut(s) 118, 579, 678
BstMWI GCNNNNNNNGC 1 cut(s) 662
BstNI CCWGG 1 cut(s) 60
BstNSI RCATGY 1 cut(s) 154
BstSCI CCNGG 1 cut(s) 58
BstSFI CTRYAG 3 cut(s) 423, 532, 549
BstUI CGCG 1 cut(s) 667
BstV1I GCAGC 2 cut(s) 409, 643
BstV2I GAAGAC 1 cut(s) 147
BstX2I RGATCY 2 cut(s) 118, 579
BstYI RGATCY 2 cut(s) 118, 579
BsuRI GGCC 2 cut(s) 34, 391
BtsCI GGATG 2 cut(s) 220, 256
BtsIMutI CAGTG 3 cut(s) 69, 167, 252
Cac8I GCNNGC 1 cut(s) 553
CciI TCATGA 1 cut(s) 81
CfoI GCGC 2 cut(s) 94, 667
Csp6I GTAC 3 cut(s) 486, 511, 601
CviAII CATG 4 cut(s) 82, 151, 168, 640
CviQI GTAC 3 cut(s) 486, 511, 601
DdeI CTNAG 5 cut(s) 110, 244, 418, 438, 583
DpnI GATC 3 cut(s) 120, 581, 680
DpnII GATC 3 cut(s) 118, 579, 678
DraI TTTAAA 1 cut(s) 234
Eam1104I CTCTTC 1 cut(s) 325
EarI CTCTTC 1 cut(s) 325
Eco147I AGGCCT 2 cut(s) 34, 391
Eco57I CTGAAG 1 cut(s) 596
EcoNI CCTNNNNNAGG 1 cut(s) 532
EcoRII CCWGG 1 cut(s) 58
EcoT22I ATGCAT 1 cut(s) 294
Esp3I CGTCTC 1 cut(s) 83
FaeI CATG 4 cut(s) 85, 154, 171, 643
FalI AAGNNNNNCTT 2 cut(s) 597, 629
FaqI GGGAC 1 cut(s) 602
FatI CATG 4 cut(s) 81, 150, 167, 639
FblI GTMKAC 1 cut(s) 400
Fnu4HI GCNGC 2 cut(s) 423, 657
FokI GGATG 2 cut(s) 227, 263
Fsp4HI GCNGC 2 cut(s) 423, 657
FspBI CTAG 2 cut(s) 266, 733
GlaI GCGC 2 cut(s) 93, 666
GluI GCNGC 2 cut(s) 423, 657
HaeIII GGCC 2 cut(s) 34, 391
HhaI GCGC 2 cut(s) 94, 667
Hin1II CATG 4 cut(s) 85, 154, 171, 643
Hin6I GCGC 2 cut(s) 92, 665
HinP1I GCGC 2 cut(s) 92, 665
HincII GTYRAC 1 cut(s) 430
HindII GTYRAC 1 cut(s) 430
HindIII AAGCTT 1 cut(s) 611
HinfI GANTC 3 cut(s) 85, 362, 443
HpaI GTTAAC 1 cut(s) 430
HphI GGTGA 2 cut(s) 16, 265
Hpy166II GTNNAC 4 cut(s) 273, 401, 430, 511
Hpy188I TCNGA 1 cut(s) 690
Hpy188III TCNNGA 3 cut(s) 82, 100, 574
Hpy8I GTNNAC 4 cut(s) 273, 401, 430, 511
HpyAV CCTTC 7 cut(s) 45, 109, 248, 275, 538, 630, 646
HpyCH4III ACNGT 1 cut(s) 162
HpyCH4IV ACGT 1 cut(s) 76
HpyCH4V TGCA 3 cut(s) 292, 425, 551
HpyF10VI GCNNNNNNNGC 1 cut(s) 662
HpyF3I CTNAG 5 cut(s) 110, 244, 418, 438, 583
HpySE526I ACGT 1 cut(s) 76
Hsp92II CATG 4 cut(s) 85, 154, 171, 643
HspAI GCGC 2 cut(s) 92, 665
KspAI GTTAAC 1 cut(s) 430
Kzo9I GATC 3 cut(s) 118, 579, 678
LmnI GCTCC 1 cut(s) 538
Lsp1109I GCAGC 2 cut(s) 409, 643
LweI GCATC 3 cut(s) 115, 550, 694
MaeI CTAG 2 cut(s) 266, 733
MaeII ACGT 1 cut(s) 76
MaeIII GTNAC 2 cut(s) 62, 181
MalI GATC 3 cut(s) 120, 581, 680
MboI GATC 3 cut(s) 118, 579, 678
MboII GAAGA 5 cut(s) 34, 47, 147, 342, 589
MflI RGATCY 2 cut(s) 118, 579
MluCI AATT 2 cut(s) 516, 563
MnlI CCTC 2 cut(s) 24, 483
Mph1103I ATGCAT 1 cut(s) 294
MseI TTAA 3 cut(s) 233, 429, 492
MspA1I CMGCKG 1 cut(s) 422
MspR9I CCNGG 1 cut(s) 60
MvaI CCWGG 1 cut(s) 60
MvnI CGCG 1 cut(s) 667
MwoI GCNNNNNNNGC 1 cut(s) 662
NdeII GATC 3 cut(s) 118, 579, 678
NlaIII CATG 4 cut(s) 85, 154, 171, 643
NmuCI GTSAC 1 cut(s) 181
NsiI ATGCAT 1 cut(s) 294
NspI RCATGY 1 cut(s) 154
NspV TTCGAA 1 cut(s) 628
PagI TCATGA 1 cut(s) 81
PceI AGGCCT 2 cut(s) 34, 391
PciI ACATGT 1 cut(s) 150
PfeI GAWTC 3 cut(s) 85, 362, 443
PflMI CCANNNNNTGG 1 cut(s) 651
PkrI GCNGC 2 cut(s) 424, 658
PscI ACATGT 1 cut(s) 150
Psp6I CCWGG 1 cut(s) 58
PspGI CCWGG 1 cut(s) 58
PstI CTGCAG 2 cut(s) 427, 553
PsuI RGATCY 2 cut(s) 118, 579
PvuII CAGCTG 1 cut(s) 422
RsaI GTAC 3 cut(s) 487, 512, 602
RsaNI GTAC 3 cut(s) 486, 511, 601
SaqAI TTAA 3 cut(s) 233, 429, 492
SatI GCNGC 2 cut(s) 423, 657
Sau3AI GATC 3 cut(s) 118, 579, 678
ScaI AGTACT 1 cut(s) 487
ScrFI CCNGG 1 cut(s) 60
SfaNI GCATC 3 cut(s) 115, 550, 694
SfcI CTRYAG 3 cut(s) 423, 532, 549
SfuI TTCGAA 1 cut(s) 628
Sse9I AATT 2 cut(s) 516, 563
SseBI AGGCCT 2 cut(s) 34, 391
SsiI CCGC 2 cut(s) 305, 730
SspMI CTAG 2 cut(s) 266, 733
StuI AGGCCT 2 cut(s) 34, 391
StyD4I CCNGG 1 cut(s) 58
TaaI ACNGT 1 cut(s) 162
TaiI ACGT 1 cut(s) 79
TaqI TCGA 2 cut(s) 312, 628
TasI AATT 2 cut(s) 516, 563
TatI WGTACW 2 cut(s) 485, 510
TfiI GAWTC 3 cut(s) 85, 362, 443
Tru1I TTAA 3 cut(s) 233, 429, 492
Tru9I TTAA 3 cut(s) 233, 429, 492
TscAI CASTG 3 cut(s) 76, 167, 252
TseFI GTSAC 1 cut(s) 181
TseI GCWGC 2 cut(s) 422, 656
Tsp45I GTSAC 1 cut(s) 181
TspDTI ATGAA 7 cut(s) 27, 156, 267, 294, 363, 576, 628
TspRI CASTG 3 cut(s) 76, 167, 252
Van91I CCANNNNNTGG 1 cut(s) 651
XagI CCTNNNNNAGG 1 cut(s) 532
XapI RAATTY 1 cut(s) 563
XceI RCATGY 1 cut(s) 154
XmiI GTMKAC 1 cut(s) 400
XspI CTAG 2 cut(s) 266, 733
ZrmI AGTACT 1 cut(s) 487
Zsp2I ATGCAT 1 cut(s) 294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.