FvH4_2g40270

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000320
Physical Location & Seq
Reverse (-)
28723626 .. 28726774
3149 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g40270.t1

Sequence Viewer

Length: 1851 bp
ATGCATTACTTCACCAATCGAAGAGGTCGACTAACAACAATTATGAAATCCCTAAACAGAAGTAGTTCAGTAATGTCGCCAAGGATTCTAGTATTTGCAAGTCTTATCCTGCCCTTCTTTGTCTCTATGGCTCTAGCCGAAGTGCATTACTATGATTTTATAGTAAAGGAGGTAAACTTCACCAGATTATGTGAAAACAAGCTCATGTTGGTTGTAAACGAAAGTTATCCAGGTCCACCAATCCGAGCTCGCAAAGGGGATACCATATATGTTAATGTTTATAATGAAGGATTTTATGGCTTTACCATTCATTGGCATGGAGTCAAGCAACCAAGAAACCCTTGGTTTGATGGCCCTGAATATGTAACACAGTGTCCAATTGCACCCGGTACAAATTTTACCTATCAAGTTCAGTTGACCGAGGAAGAAGGAACTGTATGGTGGCATGCTCATAGTGACTGGACAAGAGCCAGTGTTCACGGTGCTATTGTAGTTTTGCCTGCTCTTGGAACCATGTTTCCATTTCCAGAGCCAGATGAAGAGGAGATCATTGTTTTCGGATCTTGGTACCTGGGAAATTTGAGAGAACGAGTTGAGGAGTCTTTGCAGCCTGGAAGTACCGAGACTCTACCCCAGTCAGATGCTTACACCATCAATGGCCAACCCGGCGATTTTCTTCCATGCTCCATAAATGCAACATGGCGTCGTAAGGTGGATCACGGCAAGACGTATCTTCTTCGCCTGGTGAATGCTAACATCGATGCAGAGTTTTTCTTTGCAATTGCTGAGCACAACCTAACTGTGGTCGGACTGGACGGAGCCTACACAAAACCGATCAACACCAGATATATAGTTGTCACTCCGGGACAAACAATGGATGTGTTGCTCTATGCAAATTCTACTCCGGGGCTCTATTACATGGCTGGTAGACAATACCTGAGTACCACATCCGACACCGGTTTCGACCATATGAATGCCACTGCAATCCTTCAATATAATGGCAACTATACCACAACCGACGCTCCTTTATTTCCCGAGCACCTTCCCATGTACATGTCCCAAGCAGAAGCCTTCAATTTCACCAATAAGATTAGAAGCTTAGCCTCACCCGAACATCCCGTTAACGTCCCAAAGGAGAGTGAAATTACTACAAGAATGTTCATAACAGCTTCCATGAACTCTCTTTACTGTGCTCCAGATTTAGGTCCTACATGCGTAAACATAAGCTTCGCTACAAGCGTCAATAACATCAGCTGGGTCAATCCAACTACAGACTCTATCTTGCAAGCCTACTACAGGAACATAAGCAACCCGATTTATGAAACAGATTTCCCGGACCAGCCGCCTACTTATTTTAATTTCACAGATGGAGGGACGACGATGTCTACTGTGCTGACAGTTCAAGGGACAAAGGTTAAGGTGCTAGACTACAATGAAACCGTTGAGATTGTGTTCCAAGGGACTAATGTGATGGGAGGTGCCGTGAATCATCCAATGCATTTGCATGGGCATAGTTTCTATGTGCTCGGATTTGGTTTCGGACCCTTTGATCCAGTGAAGGACCCTAAAGGCTATAATTTGGTCGATCCTCCTTATGTTGCTACATTCGTCACTCCCAAAAATGGATGGCTAACTATCAGATTTGTAGCTGATAATCCTGGGGTGTGGTTCTGGCATTGTCATGTAGATAGACATATGACATGGGGTATGGAGGCGGCGTTTATAATCAAGGACGGGGGCACTGCTGAGACTAGTATGCTTCCACCACCAGCTCAAATGCCTTCCTGTGATGTTCCATTGGATTCTGCTATCAGAAATTACGAGGCCTTGATTGAAAAGCAAATGGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00053 ko01100 map00053 map01100
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

617

Amino Acids

69.37

Weight (kDa)

5.38

Isoelectric Point (pI)

45.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_3 PF07732 55 - 167 6.9e-38 Multicopper oxidase
Cu-oxidase PF00394 182 - 335 2e-33 Multicopper oxidase
Cu-oxidase_2 PF07731 443 - 578 9.7e-41 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000320)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g12640 FvH4_2g18980 FvH4_2g18990 FvH4_2g40270 FvH4_2g40270 FvH4_4g22740 FvH4_4g22740 FvH4_5g35760 FvH4_5g35760 FvH4_5g35760 FvH4_5g35760 FvH4_5g35760 FvH4_5g35760 FvH4_5g35760 FvH4_5g35760 FvH4_5g36460 FvH4_5g36460 FvH4_6g39950 FvH4_6g39970 FvH4_6g39983 FvH4_6g39990
rosa_chinensis RchiOBHm_Chr2g0154481 RchiOBHm_Chr2g0154511 RchiOBHm_Chr4g0428961 RchiOBHm_Chr6g0252431 RchiOBHm_Chr6g0273301 RchiOBHm_Chr6g0273631 RchiOBHm_Chr6g0273651 RchiOBHm_Chr6g0284751 RchiOBHm_Chr6g0284761 RchiOBHm_Chr6g0300491 RchiOBHm_Chr7g0237421 RchiOBHm_Chr7g0238581
rosa_laevigata RLG00000000906 RLG00000001006 RLG00000007110 RLG00000011333 RLG00000012721 RLG00000013605 RLG00000013626 RLG00000020780 RLG00000020782 RLG00000020783 RLG00000020786
rosa_multiflora Rmu_co8321753.1_g000001 Rmu_sc0000239.1_g000020 Rmu_sc0000686.1_g000001 Rmu_sc0000686.1_g000003 Rmu_sc0000686.1_g000005 Rmu_sc0001476.1_g000011 Rmu_sc0002231.1_g000002 Rmu_sc0002231.1_g000016 Rmu_sc0002690.1_g000003 Rmu_sc0002717.1_g000015 Rmu_sc0002923.1_g000026 Rmu_sc0003808.1_g000016 Rmu_sc0006475.1_g000008 Rmu_sc0006475.1_g000012 Rmu_sc0006475.1_g000018 Rmu_sc0014815.1_g000004 Rmu_sc0015313.1_g000012
rosa_roxburghii Rroxscaffold_2G00094270 Rroxscaffold_2G00094290 Rroxscaffold_3G00222950 Rroxscaffold_3G00224250 Rroxscaffold_5G00370630 Rroxscaffold_7G00167740 Rroxscaffold_7G00184410 Rroxscaffold_7G00195190 Rroxscaffold_7G00195460
rosa_rugosa Rorug02G0444600 Rorug04G0231100 Rorug04G0231200 Rorug06G0074000 Rorug06G0076800 Rorug06G0166400 Rorug06G0166500 Rorug06G0166600 Rorug06G0296400 Rorug07G0303000
rosa_samantha Rh2CG494600 Rh2CG494700 Rh2DG530800 Rh2DG530900 Rh2DG531000 Rh4AG287500 Rh4AG403800 Rh4BG293400 Rh4BG415100 Rh4DG290500 Rh6AG191000 Rh6AG191300 Rh6BG192500 Rh6BG194800 Rh6BG195200 Rh6BG279500 Rh6BG279600 Rh6DG183900 Rh6DG186600 Rh6DG274300 Rh6DG274500 Rh6DG409500 Rh7AG460600 Rh7AG468200 Rh7BG430200
rosa_wichuraiana Rw2G041820 Rw2G041830 Rw2G041840 Rw4G024930 Rw6G016470 Rw6G016720 Rw6G016730 Rw6G024020 Rw6G024030 Rw6G035790 Rw7G038130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 282, 1724
AasI GACNNNNNNGTC 1 cut(s) 1381
Acc65I GGTACC 1 cut(s) 567
AccB1I GGYRCC 2 cut(s) 567, 1478
AccB7I CCANNNNNTGG 1 cut(s) 312
AccI GTMKAC 3 cut(s) 28, 928, 1385
AciI CCGC 2 cut(s) 1343, 1715
AclWI GGATC 4 cut(s) 568, 723, 1544, 1580
AcoI YGGCCR 1 cut(s) 658
AcsI RAATTY 3 cut(s) 394, 577, 895
AcyI GRCGYC 1 cut(s) 703
AfaI GTAC 5 cut(s) 391, 569, 619, 943, 1052
AfiI CCNNNNNNNGG 6 cut(s) 312, 506, 802, 1202, 1296, 1622
AflIII ACRYGT 1 cut(s) 1053
AgeI ACCGGT 1 cut(s) 956
AgsI TTSAA 4 cut(s) 992, 1075, 1403, 1835
AhlI ACTAGT 1 cut(s) 1751
AjnI CCWGG 5 cut(s) 229, 570, 610, 741, 1657
AloI GAACNNNNNNTCC 2 cut(s) 502, 534
AluBI AGCT 8 cut(s) 202, 248, 1098, 1169, 1227, 1254, 1649, 1772
AluI AGCT 8 cut(s) 202, 248, 1098, 1169, 1227, 1254, 1649, 1772
Alw21I GWGCWC 5 cut(s) 250, 792, 1041, 1195, 1527
Alw26I GTCTC 3 cut(s) 127, 617, 1742
AlwI GGATC 4 cut(s) 568, 723, 1544, 1580
Ama87I CYCGRG 1 cut(s) 1034
AoxI GGCC 3 cut(s) 352, 658, 1824
ApeKI GCWGC 1 cut(s) 607
ApoI RAATTY 3 cut(s) 394, 577, 895
ArsI GACNNNNNNTTYG 2 cut(s) 944, 976
AsiGI ACCGGT 1 cut(s) 956
Asp700I GAANNNNTTC 1 cut(s) 64
Asp718I GGTACC 1 cut(s) 567
AspS9I GGNCC 6 cut(s) 233, 353, 1205, 1336, 1541, 1561
AsuC2I CCSGG 5 cut(s) 387, 666, 864, 906, 1334
AsuHPI GGTGA 5 cut(s) 4, 172, 757, 1072, 1098
AvaI CYCGRG 1 cut(s) 1034
AvaII GGWCC 5 cut(s) 233, 1205, 1336, 1541, 1561
BaeGI GKGCMC 1 cut(s) 1742
BaeI ACNNNNGTAYC 2 cut(s) 925, 958
BalI TGGCCA 1 cut(s) 660
BanI GGYRCC 2 cut(s) 567, 1478
BanII GRGCYC 2 cut(s) 250, 912
Bbv12I GWGCWC 5 cut(s) 250, 792, 1041, 1195, 1527
BbvI GCAGC 1 cut(s) 619
BccI CCATC 5 cut(s) 344, 659, 1361, 1465, 1620
BceAI ACGGC 2 cut(s) 736, 1466
BciT130I CCWGG 5 cut(s) 231, 572, 612, 743, 1659
BciVI GTATCC 1 cut(s) 253
BcnI CCSGG 5 cut(s) 387, 666, 864, 906, 1334
BcoDI GTCTC 3 cut(s) 127, 617, 1742
BcuI ACTAGT 1 cut(s) 1751
BfaI CTAG 4 cut(s) 89, 134, 1424, 1752
BfmI CTRYAG 2 cut(s) 1269, 1294
BfuI GTATCC 1 cut(s) 253
BglI GCCNNNNNGGC 1 cut(s) 666
BisI GCNGC 3 cut(s) 608, 1343, 1716
BlpI GCTNAGC 2 cut(s) 786, 1099
BlsI GCNGC 3 cut(s) 609, 1344, 1717
Bme18I GGWCC 5 cut(s) 233, 1205, 1336, 1541, 1561
BmeT110I CYCGRG 1 cut(s) 1034
BmgT120I GGNCC 6 cut(s) 233, 353, 1205, 1336, 1541, 1561
BmiI GGNNCC 6 cut(s) 511, 569, 820, 1480, 1543, 1563
BmrI ACTGGG 1 cut(s) 628
BmsI GCATC 2 cut(s) 631, 751
BmuI ACTGGG 1 cut(s) 628
BpmI CTGGAG 1 cut(s) 1179
Bpu1102I GCTNAGC 2 cut(s) 786, 1099
BpuMI CCSGG 5 cut(s) 387, 666, 864, 906, 1334
Bsa29I ATCGAT 1 cut(s) 759
BsaHI GRCGYC 1 cut(s) 703
BsaJI CCNNGG 7 cut(s) 80, 341, 420, 571, 905, 1456, 1658
BsaWI WCCGGW 1 cut(s) 956
BsaXI ACNNNNNCTCC 6 cut(s) 1006, 1036, 1467, 1497, 1703, 1733
Bsc4I CCNNNNNNNGG 6 cut(s) 312, 506, 802, 1202, 1296, 1622
Bse118I RCCGGY 1 cut(s) 956
Bse1I ACTGG 5 cut(s) 464, 471, 634, 816, 1553
BseBI CCWGG 5 cut(s) 231, 572, 612, 743, 1659
BseCI ATCGAT 1 cut(s) 759
BseDI CCNNGG 7 cut(s) 80, 341, 420, 571, 905, 1456, 1658
BseGI GGATG 5 cut(s) 883, 947, 1114, 1489, 1631
BseLI CCNNNNNNNGG 6 cut(s) 312, 506, 802, 1202, 1296, 1622
BseMII CTCAG 3 cut(s) 777, 929, 1737
BseNI ACTGG 5 cut(s) 464, 471, 634, 816, 1553
BseRI GAGGAG 2 cut(s) 557, 611
BseSI GKGCMC 1 cut(s) 1742
BseXI GCAGC 1 cut(s) 619
BseYI CCCAGC 1 cut(s) 1254
BshFI GGCC 3 cut(s) 354, 660, 1826
BshNI GGYRCC 2 cut(s) 567, 1478
BshTI ACCGGT 1 cut(s) 956
BshVI ATCGAT 1 cut(s) 759
BsiHKAI GWGCWC 5 cut(s) 250, 792, 1041, 1195, 1527
BsiHKCI CYCGRG 1 cut(s) 1034
BsiSI CCGG 6 cut(s) 387, 666, 863, 905, 957, 1334
BslFI GGGAC 6 cut(s) 879, 1042, 1112, 1387, 1420, 1474
BslI CCNNNNNNNGG 6 cut(s) 312, 506, 802, 1202, 1296, 1622
BsmAI GTCTC 3 cut(s) 127, 617, 1742
BsmFI GGGAC 6 cut(s) 879, 1042, 1112, 1387, 1420, 1474
BsmI GAATGC 2 cut(s) 754, 979
BsnI GGCC 3 cut(s) 354, 660, 1826
BsoBI CYCGRG 1 cut(s) 1034
Bsp1286I GDGCHC 7 cut(s) 250, 792, 912, 1041, 1195, 1527, 1742
Bsp1407I TGTACA 1 cut(s) 1050
Bsp143I GATC 6 cut(s) 546, 560, 715, 834, 1549, 1585
Bsp1720I GCTNAGC 2 cut(s) 786, 1099
BspACI CCGC 2 cut(s) 1343, 1715
BspANI GGCC 3 cut(s) 354, 660, 1826
BspCNI CTCAG 3 cut(s) 778, 930, 1738
BspDI ATCGAT 1 cut(s) 759
BspLI GGNNCC 6 cut(s) 511, 569, 820, 1480, 1543, 1563
BspPI GGATC 4 cut(s) 568, 723, 1544, 1580
BspT107I GGYRCC 2 cut(s) 567, 1478
BsrFI RCCGGY 1 cut(s) 956
BsrGI TGTACA 1 cut(s) 1050
BsrI ACTGG 5 cut(s) 464, 471, 634, 816, 1553
BssAI RCCGGY 1 cut(s) 956
BssECI CCNNGG 7 cut(s) 80, 341, 420, 571, 905, 1456, 1658
BssMI GATC 6 cut(s) 546, 560, 715, 834, 1549, 1585
BssNI GRCGYC 1 cut(s) 703
BssT1I CCWWGG 3 cut(s) 80, 341, 1456
Bst2UI CCWGG 5 cut(s) 231, 572, 612, 743, 1659
Bst4CI ACNGT 8 cut(s) 372, 436, 482, 802, 1190, 1390, 1399, 1441
Bst6I CTCTTC 2 cut(s) 16, 534
BstACI GRCGYC 1 cut(s) 703
BstAUI TGTACA 1 cut(s) 1050
BstC8I GCNNGC 4 cut(s) 250, 447, 501, 1287
BstDEI CTNAG 4 cut(s) 786, 938, 1099, 1746
BstENI CCTNNNNNAGG 1 cut(s) 1294
BstF5I GGATG 5 cut(s) 883, 947, 1114, 1489, 1631
BstKTI GATC 6 cut(s) 549, 563, 718, 837, 1552, 1588
BstMAI GTCTC 3 cut(s) 127, 617, 1742
BstMBI GATC 6 cut(s) 546, 560, 715, 834, 1549, 1585
BstMWI GCNNNNNNNGC 1 cut(s) 666
BstNI CCWGG 5 cut(s) 231, 572, 612, 743, 1659
BstNSI RCATGY 3 cut(s) 449, 1057, 1215
BstSFI CTRYAG 2 cut(s) 1269, 1294
BstSLI GKGCMC 1 cut(s) 1742
BstV1I GCAGC 1 cut(s) 619
BstX2I RGATCY 1 cut(s) 560
BstYI RGATCY 1 cut(s) 560
Bsu15I ATCGAT 1 cut(s) 759
BsuI GTATCC 1 cut(s) 253
BsuRI GGCC 3 cut(s) 354, 660, 1826
BsuTUI ATCGAT 1 cut(s) 759
BtsCI GGATG 5 cut(s) 883, 947, 1114, 1489, 1631
BtsI GCAGTG 2 cut(s) 978, 1740
BtsIMutI CAGTG 5 cut(s) 377, 478, 978, 1560, 1740
Cac8I GCNNGC 4 cut(s) 250, 447, 501, 1287
Cfr10I RCCGGY 1 cut(s) 956
Cfr13I GGNCC 6 cut(s) 233, 353, 1205, 1336, 1541, 1561
ClaI ATCGAT 1 cut(s) 759
CseI GACGC 3 cut(s) 692, 1028, 1228
Csp6I GTAC 5 cut(s) 390, 568, 618, 942, 1051
CspAI ACCGGT 1 cut(s) 956
CviQI GTAC 5 cut(s) 390, 568, 618, 942, 1051
DdeI CTNAG 4 cut(s) 786, 938, 1099, 1746
DpnI GATC 6 cut(s) 548, 562, 717, 836, 1551, 1587
DpnII GATC 6 cut(s) 546, 560, 715, 834, 1549, 1585
DrdI GACNNNNNNGTC 1 cut(s) 1381
DseDI GACNNNNNNGTC 1 cut(s) 1381
EaeI YGGCCR 1 cut(s) 658
Eam1104I CTCTTC 2 cut(s) 16, 534
EarI CTCTTC 2 cut(s) 16, 534
Ecl136II GAGCTC 1 cut(s) 248
Eco130I CCWWGG 3 cut(s) 80, 341, 1456
Eco147I AGGCCT 1 cut(s) 1826
Eco24I GRGCYC 2 cut(s) 250, 912
Eco47I GGWCC 5 cut(s) 233, 1205, 1336, 1541, 1561
Eco53kI GAGCTC 1 cut(s) 248
Eco88I CYCGRG 1 cut(s) 1034
EcoICRI GAGCTC 1 cut(s) 248
EcoNI CCTNNNNNAGG 1 cut(s) 1294
EcoO109I RGGNCCY 2 cut(s) 1205, 1561
EcoRII CCWGG 5 cut(s) 229, 570, 610, 741, 1657
EcoT14I CCWWGG 3 cut(s) 80, 341, 1456
EcoT22I ATGCAT 2 cut(s) 6, 1500
EcoT38I GRGCYC 2 cut(s) 250, 912
ErhI CCWWGG 3 cut(s) 80, 341, 1456
FalI AAGNNNNNCTT 2 cut(s) 325, 357
FaqI GGGAC 6 cut(s) 879, 1042, 1112, 1387, 1420, 1474
FauNDI CATATG 2 cut(s) 969, 1695
FblI GTMKAC 3 cut(s) 28, 928, 1385
Fnu4HI GCNGC 3 cut(s) 608, 1343, 1716
FokI GGATG 5 cut(s) 890, 934, 1101, 1476, 1638
FriOI GRGCYC 2 cut(s) 250, 912
Fsp4HI GCNGC 3 cut(s) 608, 1343, 1716
FspBI CTAG 4 cut(s) 89, 134, 1424, 1752
GluI GCNGC 3 cut(s) 608, 1343, 1716
GsaI CCCAGC 1 cut(s) 1258
GsuI CTGGAG 1 cut(s) 1179
HaeIII GGCC 3 cut(s) 354, 660, 1826
HapII CCGG 6 cut(s) 387, 666, 863, 905, 957, 1334
HgaI GACGC 3 cut(s) 692, 1028, 1228
Hin1I GRCGYC 1 cut(s) 703
HincII GTYRAC 3 cut(s) 29, 417, 1123
HindII GTYRAC 3 cut(s) 29, 417, 1123
HindIII AAGCTT 2 cut(s) 1096, 1225
HinfI GANTC 7 cut(s) 85, 321, 599, 625, 1274, 1486, 1802
HpaI GTTAAC 1 cut(s) 1123
HpaII CCGG 6 cut(s) 387, 666, 863, 905, 957, 1334
HphI GGTGA 5 cut(s) 4, 172, 757, 1072, 1098
Hpy188I TCNGA 9 cut(s) 245, 560, 640, 809, 952, 1529, 1541, 1640, 1814
Hpy188III TCNNGA 3 cut(s) 527, 1034, 1196
Hpy99I CGWCG 3 cut(s) 708, 1022, 1381
HpyAV CCTTC 8 cut(s) 124, 281, 422, 998, 1052, 1081, 1552, 1791
HpyCH4III ACNGT 8 cut(s) 372, 436, 482, 802, 1190, 1390, 1399, 1441
HpyCH4IV ACGT 2 cut(s) 728, 1125
HpyF10VI GCNNNNNNNGC 1 cut(s) 666
HpyF3I CTNAG 4 cut(s) 786, 938, 1099, 1746
HpySE526I ACGT 2 cut(s) 728, 1125
Hsp92I GRCGYC 1 cut(s) 703
KpnI GGTACC 1 cut(s) 571
KspAI GTTAAC 1 cut(s) 1123
Kzo9I GATC 6 cut(s) 546, 560, 715, 834, 1549, 1585
LmnI GCTCC 4 cut(s) 689, 818, 1027, 1198
Lsp1109I GCAGC 1 cut(s) 619
LweI GCATC 2 cut(s) 631, 751
MaeI CTAG 4 cut(s) 89, 134, 1424, 1752
MaeII ACGT 2 cut(s) 728, 1125
MaeIII GTNAC 4 cut(s) 364, 455, 856, 1609
MalI GATC 6 cut(s) 548, 562, 717, 836, 1551, 1587
MboI GATC 6 cut(s) 546, 560, 715, 834, 1549, 1585
MboII GAAGA 6 cut(s) 33, 437, 551, 668, 725, 728
MfeI CAATTG 2 cut(s) 378, 780
MflI RGATCY 1 cut(s) 560
MhlI GDGCHC 7 cut(s) 250, 792, 912, 1041, 1195, 1527, 1742
MlsI TGGCCA 1 cut(s) 660
MluNI TGGCCA 1 cut(s) 660
MlyI GAGTC 4 cut(s) 330, 608, 619, 1268
MmeI TCCRAC 3 cut(s) 787, 975, 1289
Mox20I TGGCCA 1 cut(s) 660
Mph1103I ATGCAT 2 cut(s) 6, 1500
MroXI GAANNNNTTC 1 cut(s) 64
MscI TGGCCA 1 cut(s) 660
MseI TTAA 4 cut(s) 273, 1122, 1356, 1416
MslI CAYNNNNRTG 6 cut(s) 150, 315, 972, 1052, 1503, 1680
Msp20I TGGCCA 1 cut(s) 660
MspA1I CMGCKG 1 cut(s) 1254
MspI CCGG 6 cut(s) 387, 666, 863, 905, 957, 1334
MunI CAATTG 2 cut(s) 378, 780
Mva1269I GAATGC 2 cut(s) 754, 979
MvaI CCWGG 5 cut(s) 231, 572, 612, 743, 1659
MwoI GCNNNNNNNGC 1 cut(s) 666
NciI CCSGG 5 cut(s) 387, 666, 864, 906, 1334
NdeI CATATG 2 cut(s) 969, 1695
NdeII GATC 6 cut(s) 546, 560, 715, 834, 1549, 1585
NlaIV GGNNCC 6 cut(s) 511, 569, 820, 1480, 1543, 1563
NmuCI GTSAC 3 cut(s) 455, 856, 1609
NsiI ATGCAT 2 cut(s) 6, 1500
NspI RCATGY 3 cut(s) 449, 1057, 1215
PaeI GCATGC 1 cut(s) 449
PceI AGGCCT 1 cut(s) 1826
PciI ACATGT 1 cut(s) 1053
PcsI WCGNNNNNNNCGW 2 cut(s) 25, 1236
PctI GAATGC 2 cut(s) 754, 979
PdmI GAANNNNTTC 1 cut(s) 64
PfeI GAWTC 3 cut(s) 85, 1486, 1802
PflFI GACNNNGTC 1 cut(s) 1381
PflMI CCANNNNNTGG 1 cut(s) 312
PfoI TCCNGGA 2 cut(s) 862, 1332
PinAI ACCGGT 1 cut(s) 956
PkrI GCNGC 3 cut(s) 609, 1344, 1717
PleI GAGTC 4 cut(s) 329, 607, 619, 1268
PpsI GAGTC 4 cut(s) 329, 607, 619, 1268
PpuMI RGGWCCY 2 cut(s) 1205, 1561
PscI ACATGT 1 cut(s) 1053
PsiI TTATAA 2 cut(s) 282, 1724
Psp124BI GAGCTC 1 cut(s) 250
Psp5II RGGWCCY 2 cut(s) 1205, 1561
Psp6I CCWGG 5 cut(s) 229, 570, 610, 741, 1657
PspFI CCCAGC 1 cut(s) 1254
PspGI CCWGG 5 cut(s) 229, 570, 610, 741, 1657
PspN4I GGNNCC 6 cut(s) 511, 569, 820, 1480, 1543, 1563
PspPI GGNCC 6 cut(s) 233, 353, 1205, 1336, 1541, 1561
PspPPI RGGWCCY 2 cut(s) 1205, 1561
PsrI GAACNNNNNNTAC 2 cut(s) 1169, 1201
PsuI RGATCY 1 cut(s) 560
PsyI GACNNNGTC 1 cut(s) 1381
PvuII CAGCTG 1 cut(s) 1254
RsaI GTAC 5 cut(s) 391, 569, 619, 943, 1052
RsaNI GTAC 5 cut(s) 390, 568, 618, 942, 1051
RseI CAYNNNNRTG 6 cut(s) 150, 315, 972, 1052, 1503, 1680
SacI GAGCTC 1 cut(s) 250
SalI GTCGAC 1 cut(s) 27
SaqAI TTAA 4 cut(s) 273, 1122, 1356, 1416
SatI GCNGC 3 cut(s) 608, 1343, 1716
Sau3AI GATC 6 cut(s) 546, 560, 715, 834, 1549, 1585
Sau96I GGNCC 6 cut(s) 233, 353, 1205, 1336, 1541, 1561
SchI GAGTC 4 cut(s) 330, 608, 619, 1268
SduI GDGCHC 7 cut(s) 250, 792, 912, 1041, 1195, 1527, 1742
SfaNI GCATC 2 cut(s) 631, 751
SfcI CTRYAG 2 cut(s) 1269, 1294
SinI GGWCC 5 cut(s) 233, 1205, 1336, 1541, 1561
SmiMI CAYNNNNRTG 6 cut(s) 150, 315, 972, 1052, 1503, 1680
SpeI ACTAGT 1 cut(s) 1751
SphI GCATGC 1 cut(s) 449
SseBI AGGCCT 1 cut(s) 1826
SsiI CCGC 2 cut(s) 1343, 1715
SspMI CTAG 4 cut(s) 89, 134, 1424, 1752
SstI GAGCTC 1 cut(s) 250
StuI AGGCCT 1 cut(s) 1826
StyI CCWWGG 3 cut(s) 80, 341, 1456
TaaI ACNGT 8 cut(s) 372, 436, 482, 802, 1190, 1390, 1399, 1441
TaiI ACGT 2 cut(s) 731, 1128
TaqI TCGA 5 cut(s) 19, 28, 759, 963, 1584
TaqII GACCGA 1 cut(s) 434
TatI WGTACW 1 cut(s) 1050
TauI GCSGC 2 cut(s) 1345, 1718
TfiI GAWTC 3 cut(s) 85, 1486, 1802
Tru1I TTAA 4 cut(s) 273, 1122, 1356, 1416
Tru9I TTAA 4 cut(s) 273, 1122, 1356, 1416
TscAI CASTG 5 cut(s) 377, 478, 985, 1560, 1747
TseFI GTSAC 3 cut(s) 455, 856, 1609
TseI GCWGC 1 cut(s) 607
Tsp45I GTSAC 3 cut(s) 455, 856, 1609
TspDTI ATGAA 9 cut(s) 59, 299, 300, 552, 986, 1150, 1190, 1335, 1449
TspGWI ACGGA 1 cut(s) 831
TspRI CASTG 5 cut(s) 377, 478, 985, 1560, 1747
Tth111I GACNNNGTC 1 cut(s) 1381
Van91I CCANNNNNTGG 1 cut(s) 312
VpaK11BI GGWCC 5 cut(s) 233, 1205, 1336, 1541, 1561
XagI CCTNNNNNAGG 1 cut(s) 1294
XapI RAATTY 3 cut(s) 394, 577, 895
XceI RCATGY 3 cut(s) 449, 1057, 1215
XcmI CCANNNNNNNNNTGG 1 cut(s) 339
XmiI GTMKAC 3 cut(s) 28, 928, 1385
XmnI GAANNNNTTC 1 cut(s) 64
XspI CTAG 4 cut(s) 89, 134, 1424, 1752
Zsp2I ATGCAT 2 cut(s) 6, 1500
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.