Rh6DG409500

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0000320
Physical Location & Seq
Forward (+)
59586531 .. 59590089
3559 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG409500.1

Sequence Viewer

Length: 1779 bp
ATGTCGCCACGGATTCTAGTCTTTGCTATTCTTATGCTGCCCTTCTTCGTCTCTCTGGCTCTAGCCGAAGTGCATTACTATGATTTTGTAGTAAAGGAGGTAAACTTCACCAGATTATGTGAAAATAAGCTCATGTTGGTTGTTAACGAAAGTTATCCAGGTCCAGCAATCCGAGCTCGCAAAGGGGATACAGTATACGTTAATGTTTACAATGAAGGATTTTATGGCTTCACTATTCATTGGCATGGAGTAAAGCAGCCAAGAAACCCGTGGTTTGATGGCCCTGAGTATGTGACACAGTGTCCAATTGCACCCGGTACAAATTTCACTTATCAAGTGCAATTGACTAAGGAAGAAGGAACCGTATGGTGGCATGCTCATAGTGACTGGACAAGAGCCAGTGTTCACGGTGCTATTGTAGTTTTGCCTGCTCTTGGTACCACGTTTCCATTTCCTGAGCCGGATGAAGAGGAGATCATTGTTTTCGGATCTTGGTACCTGGGAAATTTGAGAGAACGAGTTGAGGAATCTTTGCAGCCTGGAAGTACCGAGACTCTACCACAGTCAGATGCTTACACCATCAATGGCCAACCCGGCGATTTTCTTCCATGCTCCATAAATACAACATGGCGTCGTAGGGTGGACTACGGCAAGACTTATCTTCTTCGCCTGGTGAATGCTAACATCGATGCAGAGTTTTTCTTTGCAATTGCTGAGCACAACCTCACTGTGGTTGGACTGGACGGAGCCTACACAAAACCCATCAATACCAGATACATAGTGGTAACTCCTGGACAAACAATGGATGTATTGCTGAATGCAAATTCTACTCCGGGGCTCTATTACATGGCTGGTAGACAATACCTTAGTACTACATCTGACACCGGCTTCGACCATATGAATGCTACTGCAATCCTTCAATATAATGGCAACTATACCACAACCGACGCTCCTTTATTTCCCGAGCACCTTCCCATGTACATGTCCCAAGCAGAAGCGTTCAATTTCACCAATAAGATTAGAAGCTTAGCCACACCAGAATATCCCGTTAATGTCCCGAAGGAGAGTGAAGTTACTACACGAATGTTCATAACAGCTTCCATGAACTCTCTTTACTGTGCTCCAGATTTAGGCCCCACCTGCTTAAACATAAGCTTTGCTACAAGCGTCAATAACATCAGCTGGGTCAATCCAACTACAGACTCTATCTTGCAAGCCTACTACAAGAACATAAGCAATCCGATTTACGAAACAGATTTTCCAGACGAGCCGCCTACTTATTATAACTTCACAGATGGAGGGACGACGCTTTCTACAGTGTTGACGATCCAAGGTACAAAGGTTAAGGTGCTAGACTATAATGAAACTGTTGAGATTGTGTTCCAAGGGACTAATGTGATGGGAGGCGCCGTGAATCATCCAATGCATTTGCATGGACATAGTTTCTACGTGCTAGGATTTGGTTTCGGATCCTTTGACCCGTTGAAGGACCCTAAAGGCTATAATTTGGTCGATCCTCCTTATGTGGCTACATTCGTTACTCCAAAAAATGGATGGCTAACAATAAGATTTGTAGCTGATAATCCTGGCGTGTGGTTTTGGCATTGTCATGTAGAGAGACATATGACATGGGGTATGGAGGCCGCTTTTATAATCAAGGACGGAGGCACAGCTGAGACTAGTATGCTACCCCCACCAGCTCAAATGCCTTCCTGTGATGTTCGATTGGATTCTGCTATCAAAAATTACGAGGCCTTGATTGAGAAGCAAATTGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00053 ko01100 map00053 map01100
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

592

Amino Acids

66.56

Weight (kDa)

5.08

Isoelectric Point (pI)

41.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_3 PF07732 31 - 143 2.2e-38 Multicopper oxidase
Cu-oxidase PF00394 158 - 311 5e-33 Multicopper oxidase
Cu-oxidase_2 PF07731 419 - 554 7.1e-40 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000320)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g12640 FvH4_2g18980 FvH4_2g18990 FvH4_2g40270 FvH4_2g40270 FvH4_4g22740 FvH4_4g22740 FvH4_5g35760 FvH4_5g35760 FvH4_5g35760 FvH4_5g35760 FvH4_5g35760 FvH4_5g35760 FvH4_5g35760 FvH4_5g35760 FvH4_5g36460 FvH4_5g36460 FvH4_6g39950 FvH4_6g39970 FvH4_6g39983 FvH4_6g39990
rosa_chinensis RchiOBHm_Chr2g0154481 RchiOBHm_Chr2g0154511 RchiOBHm_Chr4g0428961 RchiOBHm_Chr6g0252431 RchiOBHm_Chr6g0273301 RchiOBHm_Chr6g0273631 RchiOBHm_Chr6g0273651 RchiOBHm_Chr6g0284751 RchiOBHm_Chr6g0284761 RchiOBHm_Chr6g0300491 RchiOBHm_Chr7g0237421 RchiOBHm_Chr7g0238581
rosa_laevigata RLG00000000906 RLG00000001006 RLG00000007110 RLG00000011333 RLG00000012721 RLG00000013605 RLG00000013626 RLG00000020780 RLG00000020782 RLG00000020783 RLG00000020786
rosa_multiflora Rmu_co8321753.1_g000001 Rmu_sc0000239.1_g000020 Rmu_sc0000686.1_g000001 Rmu_sc0000686.1_g000003 Rmu_sc0000686.1_g000005 Rmu_sc0001476.1_g000011 Rmu_sc0002231.1_g000002 Rmu_sc0002231.1_g000016 Rmu_sc0002690.1_g000003 Rmu_sc0002717.1_g000015 Rmu_sc0002923.1_g000026 Rmu_sc0003808.1_g000016 Rmu_sc0006475.1_g000008 Rmu_sc0006475.1_g000012 Rmu_sc0006475.1_g000018 Rmu_sc0014815.1_g000004 Rmu_sc0015313.1_g000012
rosa_roxburghii Rroxscaffold_2G00094270 Rroxscaffold_2G00094290 Rroxscaffold_3G00222950 Rroxscaffold_3G00224250 Rroxscaffold_5G00370630 Rroxscaffold_7G00167740 Rroxscaffold_7G00184410 Rroxscaffold_7G00195190 Rroxscaffold_7G00195460
rosa_rugosa Rorug02G0444600 Rorug04G0231100 Rorug04G0231200 Rorug06G0074000 Rorug06G0076800 Rorug06G0166400 Rorug06G0166500 Rorug06G0166600 Rorug06G0296400 Rorug07G0303000
rosa_samantha Rh2CG494600 Rh2CG494700 Rh2DG530800 Rh2DG530900 Rh2DG531000 Rh4AG287500 Rh4AG403800 Rh4BG293400 Rh4BG415100 Rh4DG290500 Rh6AG191000 Rh6AG191300 Rh6BG192500 Rh6BG194800 Rh6BG195200 Rh6BG279500 Rh6BG279600 Rh6DG183900 Rh6DG186600 Rh6DG274300 Rh6DG274500 Rh6DG409500 Rh7AG460600 Rh7AG468200 Rh7BG430200
rosa_wichuraiana Rw2G041820 Rw2G041830 Rw2G041840 Rw4G024930 Rw6G016470 Rw6G016720 Rw6G016730 Rw6G024020 Rw6G024030 Rw6G035790 Rw7G038130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1284, 1652
AarI CACCTGC 1 cut(s) 1148
Acc36I ACCTGC 1 cut(s) 1148
Acc65I GGTACC 2 cut(s) 437, 495
AccB1I GGYRCC 3 cut(s) 437, 495, 1406
AccB7I CCANNNNNTGG 1 cut(s) 1550
AccI GTMKAC 2 cut(s) 195, 856
AciI CCGC 2 cut(s) 1271, 1644
AclWI GGATC 5 cut(s) 496, 1321, 1464, 1477, 1508
AcoI YGGCCR 1 cut(s) 586
AcsI RAATTY 3 cut(s) 322, 505, 823
AcyI GRCGYC 2 cut(s) 631, 1407
AfaI GTAC 7 cut(s) 319, 439, 497, 547, 871, 980, 1336
AfiI CCNNNNNNNGG 6 cut(s) 369, 434, 730, 1130, 1486, 1550
AflIII ACRYGT 1 cut(s) 981
AgsI TTSAA 3 cut(s) 920, 1003, 1486
AhdI GACNNNNNGTC 1 cut(s) 300
AhlI ACTAGT 1 cut(s) 1679
AjnI CCWGG 6 cut(s) 157, 498, 538, 669, 790, 1585
AluBI AGCT 9 cut(s) 130, 176, 1026, 1097, 1155, 1182, 1577, 1673, 1700
AluI AGCT 9 cut(s) 130, 176, 1026, 1097, 1155, 1182, 1577, 1673, 1700
Alw21I GWGCWC 4 cut(s) 178, 720, 969, 1123
Alw26I GTCTC 4 cut(s) 55, 545, 1612, 1670
AlwI GGATC 5 cut(s) 496, 1321, 1464, 1477, 1508
Ama87I CYCGRG 1 cut(s) 962
AoxI GGCC 5 cut(s) 280, 586, 1132, 1641, 1752
ApeKI GCWGC 3 cut(s) 37, 256, 535
ApoI RAATTY 3 cut(s) 322, 505, 823
ArsI GACNNNNNNTTYG 2 cut(s) 872, 904
Asp718I GGTACC 2 cut(s) 437, 495
AspLEI GCGC 1 cut(s) 1409
AspS9I GGNCC 4 cut(s) 161, 281, 1133, 1489
AsuC2I CCSGG 3 cut(s) 315, 594, 834
AsuHPI GGTGA 3 cut(s) 100, 685, 1000
AvaI CYCGRG 1 cut(s) 962
AvaII GGWCC 2 cut(s) 161, 1489
BaeI ACNNNNGTAYC 2 cut(s) 1326, 1359
BalI TGGCCA 1 cut(s) 588
BamHI GGATCC 1 cut(s) 1469
BanI GGYRCC 3 cut(s) 437, 495, 1406
BanII GRGCYC 2 cut(s) 178, 840
Bbv12I GWGCWC 4 cut(s) 178, 720, 969, 1123
BbvI GCAGC 3 cut(s) 24, 268, 547
BccI CCATC 6 cut(s) 272, 587, 770, 1289, 1393, 1548
BceAI ACGGC 2 cut(s) 664, 1394
BciT130I CCWGG 6 cut(s) 159, 500, 540, 671, 792, 1587
BciVI GTATCC 1 cut(s) 181
BcnI CCSGG 3 cut(s) 315, 594, 834
BcoDI GTCTC 4 cut(s) 55, 545, 1612, 1670
BcuI ACTAGT 1 cut(s) 1679
BfaI CTAG 5 cut(s) 17, 62, 1352, 1454, 1680
BfmI CTRYAG 2 cut(s) 1197, 1314
BfoI RGCGCY 1 cut(s) 1410
BfuAI ACCTGC 1 cut(s) 1148
BfuI GTATCC 1 cut(s) 181
BglI GCCNNNNNGGC 1 cut(s) 594
BisI GCNGC 5 cut(s) 38, 257, 536, 1271, 1644
BlpI GCTNAGC 2 cut(s) 714, 1027
BlsI GCNGC 5 cut(s) 39, 258, 537, 1272, 1645
BmcAI AGTACT 1 cut(s) 871
Bme1390I CCNGG 9 cut(s) 159, 315, 500, 540, 594, 671, 792, 834, 1587
Bme18I GGWCC 2 cut(s) 161, 1489
BmeRI GACNNNNNGTC 1 cut(s) 300
BmeT110I CYCGRG 1 cut(s) 962
BmgT120I GGNCC 4 cut(s) 161, 281, 1133, 1489
BmiI GGNNCC 8 cut(s) 361, 439, 497, 748, 1135, 1408, 1471, 1491
BmrFI CCNGG 9 cut(s) 159, 315, 500, 540, 594, 671, 792, 834, 1587
BmsI GCATC 2 cut(s) 559, 679
BpmI CTGGAG 1 cut(s) 1107
Bpu10I CCTNAGC 1 cut(s) 456
Bpu1102I GCTNAGC 2 cut(s) 714, 1027
BpuMI CCSGG 3 cut(s) 315, 594, 834
Bsa29I ATCGAT 1 cut(s) 687
BsaAI YACGTR 1 cut(s) 1450
BsaHI GRCGYC 2 cut(s) 631, 1407
BsaJI CCNNGG 6 cut(s) 8, 269, 499, 833, 1330, 1384
BsaXI ACNNNNNCTCC 4 cut(s) 934, 964, 1395, 1425
Bsc4I CCNNNNNNNGG 6 cut(s) 369, 434, 730, 1130, 1486, 1550
Bse118I RCCGGY 1 cut(s) 884
Bse1I ACTGG 3 cut(s) 392, 399, 744
BseBI CCWGG 6 cut(s) 159, 500, 540, 671, 792, 1587
BseCI ATCGAT 1 cut(s) 687
BseDI CCNNGG 6 cut(s) 8, 269, 499, 833, 1330, 1384
BseGI GGATG 4 cut(s) 469, 811, 1417, 1559
BseLI CCNNNNNNNGG 6 cut(s) 369, 434, 730, 1130, 1486, 1550
BseMII CTCAG 4 cut(s) 276, 447, 705, 1665
BseNI ACTGG 3 cut(s) 392, 399, 744
BseRI GAGGAG 1 cut(s) 485
BseXI GCAGC 3 cut(s) 24, 268, 547
BseYI CCCAGC 1 cut(s) 1182
BshFI GGCC 5 cut(s) 282, 588, 1134, 1643, 1754
BshNI GGYRCC 3 cut(s) 437, 495, 1406
BshVI ATCGAT 1 cut(s) 687
BsiHKAI GWGCWC 4 cut(s) 178, 720, 969, 1123
BsiHKCI CYCGRG 1 cut(s) 962
BsiSI CCGG 5 cut(s) 315, 461, 594, 833, 885
BslFI GGGAC 4 cut(s) 970, 1040, 1315, 1402
BslI CCNNNNNNNGG 6 cut(s) 369, 434, 730, 1130, 1486, 1550
BsmAI GTCTC 4 cut(s) 55, 545, 1612, 1670
BsmBI CGTCTC 1 cut(s) 55
BsmFI GGGAC 4 cut(s) 970, 1040, 1315, 1402
BsmI GAATGC 3 cut(s) 682, 823, 907
BsnI GGCC 5 cut(s) 282, 588, 1134, 1643, 1754
BsoBI CYCGRG 1 cut(s) 962
Bsp1286I GDGCHC 5 cut(s) 178, 720, 840, 969, 1123
Bsp1407I TGTACA 1 cut(s) 978
Bsp143I GATC 5 cut(s) 474, 488, 1326, 1469, 1513
Bsp1720I GCTNAGC 2 cut(s) 714, 1027
BspACI CCGC 2 cut(s) 1271, 1644
BspANI GGCC 5 cut(s) 282, 588, 1134, 1643, 1754
BspCNI CTCAG 4 cut(s) 277, 448, 706, 1666
BspDI ATCGAT 1 cut(s) 687
BspLI GGNNCC 8 cut(s) 361, 439, 497, 748, 1135, 1408, 1471, 1491
BspMI ACCTGC 1 cut(s) 1148
BspPI GGATC 5 cut(s) 496, 1321, 1464, 1477, 1508
BspT107I GGYRCC 3 cut(s) 437, 495, 1406
BsrFI RCCGGY 1 cut(s) 884
BsrGI TGTACA 1 cut(s) 978
BsrI ACTGG 3 cut(s) 392, 399, 744
BssAI RCCGGY 1 cut(s) 884
BssECI CCNNGG 6 cut(s) 8, 269, 499, 833, 1330, 1384
BssMI GATC 5 cut(s) 474, 488, 1326, 1469, 1513
BssNAI GTATAC 1 cut(s) 196
BssNI GRCGYC 2 cut(s) 631, 1407
BssT1I CCWWGG 2 cut(s) 1330, 1384
Bst1107I GTATAC 1 cut(s) 196
Bst2UI CCWGG 6 cut(s) 159, 500, 540, 671, 792, 1587
Bst4CI ACNGT 9 cut(s) 193, 300, 364, 410, 564, 730, 1118, 1318, 1369
Bst6I CTCTTC 1 cut(s) 462
BstACI GRCGYC 2 cut(s) 631, 1407
BstAUI TGTACA 1 cut(s) 978
BstBAI YACGTR 1 cut(s) 1450
BstC8I GCNNGC 4 cut(s) 178, 375, 429, 1215
BstDEI CTNAG 7 cut(s) 285, 348, 456, 714, 866, 1027, 1674
BstDSI CCRYGG 2 cut(s) 8, 269
BstF5I GGATG 4 cut(s) 469, 811, 1417, 1559
BstH2I RGCGCY 1 cut(s) 1410
BstHHI GCGC 1 cut(s) 1409
BstKTI GATC 5 cut(s) 477, 491, 1329, 1472, 1516
BstMAI GTCTC 4 cut(s) 55, 545, 1612, 1670
BstMBI GATC 5 cut(s) 474, 488, 1326, 1469, 1513
BstMWI GCNNNNNNNGC 3 cut(s) 173, 594, 1140
BstNI CCWGG 6 cut(s) 159, 500, 540, 671, 792, 1587
BstNSI RCATGY 2 cut(s) 377, 985
BstSCI CCNGG 9 cut(s) 157, 313, 498, 538, 592, 669, 790, 832, 1585
BstSFI CTRYAG 2 cut(s) 1197, 1314
BstV1I GCAGC 3 cut(s) 24, 268, 547
BstX2I RGATCY 2 cut(s) 488, 1469
BstYI RGATCY 2 cut(s) 488, 1469
BstZ17I GTATAC 1 cut(s) 196
Bsu15I ATCGAT 1 cut(s) 687
BsuI GTATCC 1 cut(s) 181
BsuRI GGCC 5 cut(s) 282, 588, 1134, 1643, 1754
BsuTUI ATCGAT 1 cut(s) 687
BtgI CCRYGG 2 cut(s) 8, 269
BtsCI GGATG 4 cut(s) 469, 811, 1417, 1559
BtsIMutI CAGTG 4 cut(s) 305, 406, 726, 1323
BveI ACCTGC 1 cut(s) 1148
Cac8I GCNNGC 4 cut(s) 178, 375, 429, 1215
CfoI GCGC 1 cut(s) 1409
Cfr10I RCCGGY 1 cut(s) 884
Cfr13I GGNCC 4 cut(s) 161, 281, 1133, 1489
ClaI ATCGAT 1 cut(s) 687
CseI GACGC 4 cut(s) 620, 956, 1156, 1315
Csp6I GTAC 7 cut(s) 318, 438, 496, 546, 870, 979, 1335
CviQI GTAC 7 cut(s) 318, 438, 496, 546, 870, 979, 1335
DdeI CTNAG 7 cut(s) 285, 348, 456, 714, 866, 1027, 1674
DinI GGCGCC 1 cut(s) 1408
DpnI GATC 5 cut(s) 476, 490, 1328, 1471, 1515
DpnII GATC 5 cut(s) 474, 488, 1326, 1469, 1513
DriI GACNNNNNGTC 1 cut(s) 300
EaeI YGGCCR 1 cut(s) 586
Eam1104I CTCTTC 1 cut(s) 462
Eam1105I GACNNNNNGTC 1 cut(s) 300
EarI CTCTTC 1 cut(s) 462
Ecl136II GAGCTC 1 cut(s) 176
Eco130I CCWWGG 2 cut(s) 1330, 1384
Eco147I AGGCCT 1 cut(s) 1754
Eco24I GRGCYC 2 cut(s) 178, 840
Eco47I GGWCC 2 cut(s) 161, 1489
Eco53kI GAGCTC 1 cut(s) 176
Eco88I CYCGRG 1 cut(s) 962
EcoICRI GAGCTC 1 cut(s) 176
EcoO109I RGGNCCY 2 cut(s) 1133, 1489
EcoRII CCWGG 6 cut(s) 157, 498, 538, 669, 790, 1585
EcoT14I CCWWGG 2 cut(s) 1330, 1384
EcoT22I ATGCAT 1 cut(s) 1428
EcoT38I GRGCYC 2 cut(s) 178, 840
EgeI GGCGCC 1 cut(s) 1408
EheI GGCGCC 1 cut(s) 1408
ErhI CCWWGG 2 cut(s) 1330, 1384
Esp3I CGTCTC 1 cut(s) 55
FaqI GGGAC 4 cut(s) 970, 1040, 1315, 1402
FauNDI CATATG 2 cut(s) 897, 1623
FblI GTMKAC 2 cut(s) 195, 856
Fnu4HI GCNGC 5 cut(s) 38, 257, 536, 1271, 1644
FokI GGATG 4 cut(s) 476, 818, 1404, 1566
FriOI GRGCYC 2 cut(s) 178, 840
Fsp4HI GCNGC 5 cut(s) 38, 257, 536, 1271, 1644
FspBI CTAG 5 cut(s) 17, 62, 1352, 1454, 1680
GlaI GCGC 1 cut(s) 1408
GluI GCNGC 5 cut(s) 38, 257, 536, 1271, 1644
GsaI CCCAGC 1 cut(s) 1186
GsuI CTGGAG 1 cut(s) 1107
HaeII RGCGCY 1 cut(s) 1410
HaeIII GGCC 5 cut(s) 282, 588, 1134, 1643, 1754
HapII CCGG 5 cut(s) 315, 461, 594, 833, 885
HgaI GACGC 4 cut(s) 620, 956, 1156, 1315
HhaI GCGC 1 cut(s) 1409
Hin1I GRCGYC 2 cut(s) 631, 1407
Hin6I GCGC 1 cut(s) 1407
HinP1I GCGC 1 cut(s) 1407
HincII GTYRAC 2 cut(s) 145, 1323
HindII GTYRAC 2 cut(s) 145, 1323
HindIII AAGCTT 2 cut(s) 1024, 1153
HinfI GANTC 6 cut(s) 13, 527, 553, 1202, 1414, 1730
HpaI GTTAAC 1 cut(s) 145
HpaII CCGG 5 cut(s) 315, 461, 594, 833, 885
HphI GGTGA 3 cut(s) 100, 685, 1000
Hpy166II GTNNAC 8 cut(s) 103, 145, 196, 208, 406, 643, 857, 1323
Hpy188I TCNGA 6 cut(s) 173, 488, 568, 880, 1242, 1469
Hpy188III TCNNGA 5 cut(s) 455, 962, 1057, 1124, 1262
Hpy8I GTNNAC 8 cut(s) 103, 145, 196, 208, 406, 643, 857, 1323
Hpy99I CGWCG 3 cut(s) 636, 950, 1309
HpyAV CCTTC 8 cut(s) 52, 209, 350, 926, 980, 1054, 1480, 1719
HpyCH4III ACNGT 9 cut(s) 193, 300, 364, 410, 564, 730, 1118, 1318, 1369
HpyCH4IV ACGT 3 cut(s) 198, 443, 1449
HpyF10VI GCNNNNNNNGC 3 cut(s) 173, 594, 1140
HpyF3I CTNAG 7 cut(s) 285, 348, 456, 714, 866, 1027, 1674
HpySE526I ACGT 3 cut(s) 198, 443, 1449
Hsp92I GRCGYC 2 cut(s) 631, 1407
HspAI GCGC 1 cut(s) 1407
KasI GGCGCC 1 cut(s) 1406
KpnI GGTACC 2 cut(s) 441, 499
KspAI GTTAAC 1 cut(s) 145
Kzo9I GATC 5 cut(s) 474, 488, 1326, 1469, 1513
LmnI GCTCC 4 cut(s) 617, 746, 955, 1126
Lsp1109I GCAGC 3 cut(s) 24, 268, 547
LweI GCATC 2 cut(s) 559, 679
MaeI CTAG 5 cut(s) 17, 62, 1352, 1454, 1680
MaeII ACGT 3 cut(s) 198, 443, 1449
MaeIII GTNAC 5 cut(s) 292, 383, 784, 1072, 1537
MalI GATC 5 cut(s) 476, 490, 1328, 1471, 1515
MboI GATC 5 cut(s) 474, 488, 1326, 1469, 1513
MboII GAAGA 6 cut(s) 37, 365, 479, 596, 653, 656
MfeI CAATTG 3 cut(s) 306, 341, 708
MflI RGATCY 2 cut(s) 488, 1469
MhlI GDGCHC 5 cut(s) 178, 720, 840, 969, 1123
MlsI TGGCCA 1 cut(s) 588
MluNI TGGCCA 1 cut(s) 588
Mly113I GGCGCC 1 cut(s) 1407
MlyI GAGTC 2 cut(s) 547, 1196
MmeI TCCRAC 2 cut(s) 715, 1217
Mox20I TGGCCA 1 cut(s) 588
Mph1103I ATGCAT 1 cut(s) 1428
MscI TGGCCA 1 cut(s) 588
MseI TTAA 5 cut(s) 144, 201, 1050, 1145, 1344
MslI CAYNNNNRTG 6 cut(s) 78, 243, 900, 980, 1431, 1608
Msp20I TGGCCA 1 cut(s) 588
MspA1I CMGCKG 2 cut(s) 1182, 1673
MspI CCGG 5 cut(s) 315, 461, 594, 833, 885
MspR9I CCNGG 9 cut(s) 159, 315, 500, 540, 594, 671, 792, 834, 1587
MunI CAATTG 3 cut(s) 306, 341, 708
Mva1269I GAATGC 3 cut(s) 682, 823, 907
MvaI CCWGG 6 cut(s) 159, 500, 540, 671, 792, 1587
MwoI GCNNNNNNNGC 3 cut(s) 173, 594, 1140
NarI GGCGCC 1 cut(s) 1407
NciI CCSGG 3 cut(s) 315, 594, 834
NdeI CATATG 2 cut(s) 897, 1623
NdeII GATC 5 cut(s) 474, 488, 1326, 1469, 1513
NlaIV GGNNCC 8 cut(s) 361, 439, 497, 748, 1135, 1408, 1471, 1491
NmuCI GTSAC 2 cut(s) 292, 383
NsiI ATGCAT 1 cut(s) 1428
NspI RCATGY 2 cut(s) 377, 985
PaeI GCATGC 1 cut(s) 377
PaqCI CACCTGC 1 cut(s) 1148
PceI AGGCCT 1 cut(s) 1754
PciI ACATGT 1 cut(s) 981
PctI GAATGC 3 cut(s) 682, 823, 907
PfeI GAWTC 4 cut(s) 13, 527, 1414, 1730
PflMI CCANNNNNTGG 1 cut(s) 1550
PfoI TCCNGGA 1 cut(s) 790
PkrI GCNGC 5 cut(s) 39, 258, 537, 1272, 1645
PleI GAGTC 2 cut(s) 547, 1196
PluTI GGCGCC 1 cut(s) 1410
PpsI GAGTC 2 cut(s) 547, 1196
Ppu21I YACGTR 1 cut(s) 1450
PpuMI RGGWCCY 1 cut(s) 1489
PscI ACATGT 1 cut(s) 981
PsiI TTATAA 2 cut(s) 1284, 1652
Psp124BI GAGCTC 1 cut(s) 178
Psp5II RGGWCCY 1 cut(s) 1489
Psp6I CCWGG 6 cut(s) 157, 498, 538, 669, 790, 1585
PspFI CCCAGC 1 cut(s) 1182
PspGI CCWGG 6 cut(s) 157, 498, 538, 669, 790, 1585
PspN4I GGNNCC 8 cut(s) 361, 439, 497, 748, 1135, 1408, 1471, 1491
PspPI GGNCC 4 cut(s) 161, 281, 1133, 1489
PspPPI RGGWCCY 1 cut(s) 1489
PsrI GAACNNNNNNTAC 2 cut(s) 1097, 1129
PsuI RGATCY 2 cut(s) 488, 1469
PvuII CAGCTG 2 cut(s) 1182, 1673
RsaI GTAC 7 cut(s) 319, 439, 497, 547, 871, 980, 1336
RsaNI GTAC 7 cut(s) 318, 438, 496, 546, 870, 979, 1335
RseI CAYNNNNRTG 6 cut(s) 78, 243, 900, 980, 1431, 1608
SacI GAGCTC 1 cut(s) 178
SaqAI TTAA 5 cut(s) 144, 201, 1050, 1145, 1344
SatI GCNGC 5 cut(s) 38, 257, 536, 1271, 1644
Sau3AI GATC 5 cut(s) 474, 488, 1326, 1469, 1513
Sau96I GGNCC 4 cut(s) 161, 281, 1133, 1489
ScaI AGTACT 1 cut(s) 871
SchI GAGTC 2 cut(s) 547, 1196
ScrFI CCNGG 9 cut(s) 159, 315, 500, 540, 594, 671, 792, 834, 1587
SduI GDGCHC 5 cut(s) 178, 720, 840, 969, 1123
SfaNI GCATC 2 cut(s) 559, 679
SfcI CTRYAG 2 cut(s) 1197, 1314
SfoI GGCGCC 1 cut(s) 1408
SinI GGWCC 2 cut(s) 161, 1489
SmiMI CAYNNNNRTG 6 cut(s) 78, 243, 900, 980, 1431, 1608
SpeI ACTAGT 1 cut(s) 1679
SphI GCATGC 1 cut(s) 377
SseBI AGGCCT 1 cut(s) 1754
SsiI CCGC 2 cut(s) 1271, 1644
SspDI GGCGCC 1 cut(s) 1406
SspMI CTAG 5 cut(s) 17, 62, 1352, 1454, 1680
SstI GAGCTC 1 cut(s) 178
StuI AGGCCT 1 cut(s) 1754
StyD4I CCNGG 9 cut(s) 157, 313, 498, 538, 592, 669, 790, 832, 1585
StyI CCWWGG 2 cut(s) 1330, 1384
TaaI ACNGT 9 cut(s) 193, 300, 364, 410, 564, 730, 1118, 1318, 1369
TaiI ACGT 3 cut(s) 201, 446, 1452
TaqI TCGA 4 cut(s) 687, 891, 1512, 1723
TatI WGTACW 2 cut(s) 869, 978
TauI GCSGC 2 cut(s) 1273, 1646
TfiI GAWTC 4 cut(s) 13, 527, 1414, 1730
Tru1I TTAA 5 cut(s) 144, 201, 1050, 1145, 1344
Tru9I TTAA 5 cut(s) 144, 201, 1050, 1145, 1344
TscAI CASTG 4 cut(s) 305, 406, 733, 1323
TseFI GTSAC 2 cut(s) 292, 383
TseI GCWGC 3 cut(s) 37, 256, 535
Tsp45I GTSAC 2 cut(s) 292, 383
TspDTI ATGAA 7 cut(s) 227, 228, 480, 914, 1078, 1118, 1377
TspGWI ACGGA 3 cut(s) 25, 759, 1677
TspRI CASTG 4 cut(s) 305, 406, 733, 1323
Van91I CCANNNNNTGG 1 cut(s) 1550
VpaK11BI GGWCC 2 cut(s) 161, 1489
XapI RAATTY 3 cut(s) 322, 505, 823
XceI RCATGY 2 cut(s) 377, 985
XcmI CCANNNNNNNNNTGG 3 cut(s) 267, 778, 1551
XmiI GTMKAC 2 cut(s) 195, 856
XspI CTAG 5 cut(s) 17, 62, 1352, 1454, 1680
ZrmI AGTACT 1 cut(s) 871
Zsp2I ATGCAT 1 cut(s) 1428
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.