Lignin degradation and detoxification of lignin-derived products
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1509 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 1415 |
| Acc65I | GGTACC | 1 cut(s) | 569 |
| AccB1I | GGYRCC | 1 cut(s) | 569 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 267 |
| AccBSI | CCGCTC | 1 cut(s) | 1078 |
| AccI | GTMKAC | 1 cut(s) | 1194 |
| AccII | CGCG | 1 cut(s) | 1695 |
| AciI | CCGC | 4 cut(s) | 482, 1076, 1245, 1277 |
| AclWI | GGATC | 2 cut(s) | 1490, 1514 |
| AcoI | YGGCCR | 1 cut(s) | 850 |
| AcsI | RAATTY | 4 cut(s) | 1261, 1477, 1735, 1760 |
| AcuI | CTGAAG | 1 cut(s) | 1548 |
| AdeI | CACNNNGTG | 2 cut(s) | 327, 410 |
| AfaI | GTAC | 6 cut(s) | 524, 571, 644, 998, 1061, 1122 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 190, 267, 461, 1233, 1556 |
| AflIII | ACRYGT | 2 cut(s) | 199, 956 |
| AgsI | TTSAA | 7 cut(s) | 938, 1170, 1360, 1379, 1579, 1735, 1769 |
| AjnI | CCWGG | 5 cut(s) | 184, 340, 613, 805, 1591 |
| AleI | CACNNNNGTG | 1 cut(s) | 210 |
| AluBI | AGCT | 6 cut(s) | 40, 425, 764, 1044, 1449, 1752 |
| AluI | AGCT | 6 cut(s) | 40, 425, 764, 1044, 1449, 1752 |
| Alw26I | GTCTC | 7 cut(s) | 143, 743, 849, 956, 1484, 1618, 1676 |
| AlwI | GGATC | 2 cut(s) | 1490, 1514 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 902, 1307 |
| AoxI | GGCC | 1 cut(s) | 850 |
| ApeKI | GCWGC | 3 cut(s) | 610, 1010, 1013 |
| ApoI | RAATTY | 4 cut(s) | 1261, 1477, 1735, 1760 |
| Asp718I | GGTACC | 1 cut(s) | 569 |
| AspS9I | GGNCC | 3 cut(s) | 85, 188, 308 |
| AsuHPI | GGTGA | 5 cut(s) | 224, 250, 346, 1360, 1769 |
| AvaII | GGWCC | 3 cut(s) | 85, 188, 308 |
| BaeGI | GKGCMC | 1 cut(s) | 1676 |
| BalI | TGGCCA | 1 cut(s) | 852 |
| BanI | GGYRCC | 1 cut(s) | 569 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 1033, 1065 |
| BauI | CACGAG | 2 cut(s) | 136, 1740 |
| BbsI | GAAGAC | 1 cut(s) | 501 |
| BbvI | GCAGC | 3 cut(s) | 622, 1022, 1025 |
| BccI | CCATC | 6 cut(s) | 299, 319, 752, 811, 988, 1554 |
| BceAI | ACGGC | 2 cut(s) | 679, 707 |
| BciT130I | CCWGG | 5 cut(s) | 186, 342, 615, 807, 1593 |
| BcoDI | GTCTC | 7 cut(s) | 143, 743, 849, 956, 1484, 1618, 1676 |
| BfaI | CTAG | 5 cut(s) | 420, 755, 830, 1092, 1686 |
| BfmI | CTRYAG | 2 cut(s) | 894, 1231 |
| BisI | GCNGC | 4 cut(s) | 611, 1011, 1014, 1277 |
| BlsI | GCNGC | 4 cut(s) | 612, 1012, 1015, 1278 |
| Bme1390I | CCNGG | 5 cut(s) | 186, 342, 615, 807, 1593 |
| Bme18I | GGWCC | 3 cut(s) | 85, 188, 308 |
| BmgT120I | GGNCC | 3 cut(s) | 85, 188, 308 |
| BmiI | GGNNCC | 2 cut(s) | 466, 571 |
| BmrFI | CCNGG | 5 cut(s) | 186, 342, 615, 807, 1593 |
| BmsI | GCATC | 2 cut(s) | 524, 580 |
| BpiI | GAAGAC | 1 cut(s) | 501 |
| BpmI | CTGGAG | 3 cut(s) | 1038, 1614, 1662 |
| BpuEI | CTTGAG | 1 cut(s) | 865 |
| BsaAI | YACGTR | 1 cut(s) | 200 |
| BsaJI | CCNNGG | 6 cut(s) | 184, 241, 296, 806, 1390, 1500 |
| BsaWI | WCCGGW | 1 cut(s) | 190 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 190, 267, 461, 1233, 1556 |
| Bse1I | ACTGG | 3 cut(s) | 272, 841, 907 |
| BseBI | CCWGG | 5 cut(s) | 186, 342, 615, 807, 1593 |
| BseDI | CCNNGG | 6 cut(s) | 184, 241, 296, 806, 1390, 1500 |
| BseGI | GGATG | 3 cut(s) | 826, 1423, 1565 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 190, 267, 461, 1233, 1556 |
| BseMII | CTCAG | 2 cut(s) | 27, 1146 |
| BseNI | ACTGG | 3 cut(s) | 272, 841, 907 |
| BseSI | GKGCMC | 1 cut(s) | 1676 |
| BseXI | GCAGC | 3 cut(s) | 622, 1022, 1025 |
| BsgI | GTGCAG | 1 cut(s) | 86 |
| Bsh1236I | CGCG | 1 cut(s) | 1695 |
| BshFI | GGCC | 1 cut(s) | 852 |
| BshNI | GGYRCC | 1 cut(s) | 569 |
| BsiSI | CCGG | 1 cut(s) | 191 |
| BslFI | GGGAC | 3 cut(s) | 55, 1354, 1408 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 190, 267, 461, 1233, 1556 |
| BsmAI | GTCTC | 7 cut(s) | 143, 743, 849, 956, 1484, 1618, 1676 |
| BsmFI | GGGAC | 3 cut(s) | 55, 1354, 1408 |
| BsmI | GAATGC | 2 cut(s) | 709, 1689 |
| BsnI | GGCC | 1 cut(s) | 852 |
| Bsp1286I | GDGCHC | 1 cut(s) | 1676 |
| Bsp143I | GATC | 2 cut(s) | 1495, 1519 |
| Bsp19I | CCATGG | 1 cut(s) | 296 |
| Bsp68I | TCGCGA | 1 cut(s) | 1695 |
| BspACI | CCGC | 4 cut(s) | 482, 1076, 1245, 1277 |
| BspANI | GGCC | 1 cut(s) | 852 |
| BspCNI | CTCAG | 2 cut(s) | 28, 1147 |
| BspFNI | CGCG | 1 cut(s) | 1695 |
| BspLI | GGNNCC | 2 cut(s) | 466, 571 |
| BspMAI | CTGCAG | 1 cut(s) | 898 |
| BspPI | GGATC | 2 cut(s) | 1490, 1514 |
| BspT107I | GGYRCC | 1 cut(s) | 569 |
| BsrBI | CCGCTC | 1 cut(s) | 1078 |
| BsrI | ACTGG | 3 cut(s) | 272, 841, 907 |
| BssECI | CCNNGG | 6 cut(s) | 184, 241, 296, 806, 1390, 1500 |
| BssMI | GATC | 2 cut(s) | 1495, 1519 |
| BssSI | CACGAG | 2 cut(s) | 136, 1740 |
| BssT1I | CCWWGG | 4 cut(s) | 241, 296, 1390, 1500 |
| Bst2BI | CACGAG | 2 cut(s) | 136, 1740 |
| Bst2UI | CCWGG | 5 cut(s) | 186, 342, 615, 807, 1593 |
| Bst4CI | ACNGT | 9 cut(s) | 54, 220, 327, 391, 430, 647, 745, 1108, 1412 |
| Bst6I | CTCTTC | 2 cut(s) | 993, 1764 |
| BstBAI | YACGTR | 1 cut(s) | 200 |
| BstC8I | GCNNGC | 5 cut(s) | 402, 456, 631, 1141, 1697 |
| BstDEI | CTNAG | 3 cut(s) | 36, 1037, 1155 |
| BstDSI | CCRYGG | 1 cut(s) | 296 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1231 |
| BstF5I | GGATG | 3 cut(s) | 826, 1423, 1565 |
| BstFNI | CGCG | 1 cut(s) | 1695 |
| BstKTI | GATC | 2 cut(s) | 1498, 1522 |
| BstMAI | GTCTC | 7 cut(s) | 143, 743, 849, 956, 1484, 1618, 1676 |
| BstMBI | GATC | 2 cut(s) | 1495, 1519 |
| BstNI | CCWGG | 5 cut(s) | 186, 342, 615, 807, 1593 |
| BstNSI | RCATGY | 5 cut(s) | 21, 404, 633, 960, 1714 |
| BstSCI | CCNGG | 5 cut(s) | 184, 340, 613, 805, 1591 |
| BstSFI | CTRYAG | 2 cut(s) | 894, 1231 |
| BstSLI | GKGCMC | 1 cut(s) | 1676 |
| BstUI | CGCG | 1 cut(s) | 1695 |
| BstV1I | GCAGC | 3 cut(s) | 622, 1022, 1025 |
| BstV2I | GAAGAC | 1 cut(s) | 501 |
| BstX2I | RGATCY | 1 cut(s) | 1519 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 848, 1754 |
| BstYI | RGATCY | 1 cut(s) | 1519 |
| BsuRI | GGCC | 1 cut(s) | 852 |
| BtgI | CCRYGG | 1 cut(s) | 296 |
| BtsCI | GGATG | 3 cut(s) | 826, 1423, 1565 |
| BtsI | GCAGTG | 3 cut(s) | 783, 891, 1647 |
| BtsIMutI | CAGTG | 7 cut(s) | 265, 332, 741, 783, 891, 900, 1647 |
| BtuMI | TCGCGA | 1 cut(s) | 1695 |
| Cac8I | GCNNGC | 5 cut(s) | 402, 456, 631, 1141, 1697 |
| CaiI | CAGNNNCTG | 2 cut(s) | 902, 1307 |
| Cfr13I | GGNCC | 3 cut(s) | 85, 188, 308 |
| Csp6I | GTAC | 6 cut(s) | 523, 570, 643, 997, 1060, 1121 |
| CviQI | GTAC | 6 cut(s) | 523, 570, 643, 997, 1060, 1121 |
| DdeI | CTNAG | 3 cut(s) | 36, 1037, 1155 |
| DpnI | GATC | 2 cut(s) | 1497, 1521 |
| DpnII | GATC | 2 cut(s) | 1495, 1519 |
| DraI | TTTAAA | 1 cut(s) | 603 |
| DraIII | CACNNNGTG | 2 cut(s) | 327, 410 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 1415 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 1415 |
| EaeI | YGGCCR | 1 cut(s) | 850 |
| Eam1104I | CTCTTC | 2 cut(s) | 993, 1764 |
| EarI | CTCTTC | 2 cut(s) | 993, 1764 |
| Eco130I | CCWWGG | 4 cut(s) | 241, 296, 1390, 1500 |
| Eco47I | GGWCC | 3 cut(s) | 85, 188, 308 |
| Eco57I | CTGAAG | 1 cut(s) | 1548 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1231 |
| EcoRI | GAATTC | 1 cut(s) | 1735 |
| EcoRII | CCWGG | 5 cut(s) | 184, 340, 613, 805, 1591 |
| EcoT14I | CCWWGG | 4 cut(s) | 241, 296, 1390, 1500 |
| EcoT22I | ATGCAT | 2 cut(s) | 1434, 1440 |
| ErhI | CCWWGG | 4 cut(s) | 241, 296, 1390, 1500 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1234, 1266 |
| FaqI | GGGAC | 3 cut(s) | 55, 1354, 1408 |
| FauI | CCCGC | 1 cut(s) | 1238 |
| FauNDI | CATATG | 1 cut(s) | 1434 |
| FblI | GTMKAC | 1 cut(s) | 1194 |
| Fnu4HI | GCNGC | 4 cut(s) | 611, 1011, 1014, 1277 |
| FokI | GGATG | 3 cut(s) | 833, 1410, 1572 |
| Fsp4HI | GCNGC | 4 cut(s) | 611, 1011, 1014, 1277 |
| FspBI | CTAG | 5 cut(s) | 420, 755, 830, 1092, 1686 |
| GluI | GCNGC | 4 cut(s) | 611, 1011, 1014, 1277 |
| GsuI | CTGGAG | 3 cut(s) | 1038, 1614, 1662 |
| HaeIII | GGCC | 1 cut(s) | 852 |
| HapII | CCGG | 1 cut(s) | 191 |
| HincII | GTYRAC | 4 cut(s) | 30, 229, 691, 1195 |
| HindII | GTYRAC | 4 cut(s) | 30, 229, 691, 1195 |
| HindIII | AAGCTT | 1 cut(s) | 1042 |
| HinfI | GANTC | 4 cut(s) | 1256, 1304, 1370, 1575 |
| HpaII | CCGG | 1 cut(s) | 191 |
| HphI | GGTGA | 5 cut(s) | 224, 250, 346, 1360, 1769 |
| Hpy166II | GTNNAC | 7 cut(s) | 30, 205, 229, 525, 691, 1195, 1747 |
| Hpy188I | TCNGA | 3 cut(s) | 304, 589, 1574 |
| Hpy188III | TCNNGA | 4 cut(s) | 1055, 1526, 1679, 1694 |
| Hpy8I | GTNNAC | 7 cut(s) | 30, 205, 229, 525, 691, 1195, 1747 |
| Hpy99I | CGWCG | 1 cut(s) | 651 |
| HpyAV | CCTTC | 2 cut(s) | 89, 377 |
| HpyCH4III | ACNGT | 9 cut(s) | 54, 220, 327, 391, 430, 647, 745, 1108, 1412 |
| HpyCH4IV | ACGT | 2 cut(s) | 199, 231 |
| HpyF3I | CTNAG | 3 cut(s) | 36, 1037, 1155 |
| HpySE526I | ACGT | 2 cut(s) | 199, 231 |
| KpnI | GGTACC | 1 cut(s) | 573 |
| Kzo9I | GATC | 2 cut(s) | 1495, 1519 |
| LmnI | GCTCC | 2 cut(s) | 638, 761 |
| Lsp1109I | GCAGC | 3 cut(s) | 622, 1022, 1025 |
| LweI | GCATC | 2 cut(s) | 524, 580 |
| MaeI | CTAG | 5 cut(s) | 420, 755, 830, 1092, 1686 |
| MaeII | ACGT | 2 cut(s) | 199, 231 |
| MaeIII | GTNAC | 5 cut(s) | 48, 898, 922, 1130, 1531 |
| MalI | GATC | 2 cut(s) | 1497, 1521 |
| MbiI | CCGCTC | 1 cut(s) | 1078 |
| MboI | GATC | 2 cut(s) | 1495, 1519 |
| MboII | GAAGA | 6 cut(s) | 392, 506, 668, 671, 980, 1781 |
| MflI | RGATCY | 1 cut(s) | 1519 |
| MhlI | GDGCHC | 1 cut(s) | 1676 |
| MlsI | TGGCCA | 1 cut(s) | 852 |
| MluCI | AATT | 8 cut(s) | 130, 510, 1261, 1380, 1477, 1510, 1735, 1760 |
| MluNI | TGGCCA | 1 cut(s) | 852 |
| MmeI | TCCRAC | 3 cut(s) | 730, 1386, 1441 |
| MnlI | CCTC | 6 cut(s) | 83, 132, 547, 938, 1533, 1550 |
| Mox20I | TGGCCA | 1 cut(s) | 852 |
| Mph1103I | ATGCAT | 2 cut(s) | 1434, 1440 |
| MscI | TGGCCA | 1 cut(s) | 852 |
| MseI | TTAA | 3 cut(s) | 602, 771, 1631 |
| MslI | CAYNNNNRTG | 7 cut(s) | 105, 210, 270, 588, 918, 1437, 1752 |
| Msp20I | TGGCCA | 1 cut(s) | 852 |
| MspI | CCGG | 1 cut(s) | 191 |
| MspR9I | CCNGG | 5 cut(s) | 186, 342, 615, 807, 1593 |
| Mva1269I | GAATGC | 2 cut(s) | 709, 1689 |
| MvaI | CCWGG | 5 cut(s) | 186, 342, 615, 807, 1593 |
| MvnI | CGCG | 1 cut(s) | 1695 |
| NcoI | CCATGG | 1 cut(s) | 296 |
| NdeI | CATATG | 1 cut(s) | 1434 |
| NdeII | GATC | 2 cut(s) | 1495, 1519 |
| NlaIV | GGNNCC | 2 cut(s) | 466, 571 |
| NmuCI | GTSAC | 2 cut(s) | 898, 1130 |
| NruI | TCGCGA | 1 cut(s) | 1695 |
| NsiI | ATGCAT | 2 cut(s) | 1434, 1440 |
| NspI | RCATGY | 5 cut(s) | 21, 404, 633, 960, 1714 |
| OliI | CACNNNNGTG | 1 cut(s) | 210 |
| PaeI | GCATGC | 2 cut(s) | 404, 633 |
| PciI | ACATGT | 1 cut(s) | 956 |
| PctI | GAATGC | 2 cut(s) | 709, 1689 |
| PfeI | GAWTC | 4 cut(s) | 1256, 1304, 1370, 1575 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 267 |
| PfoI | TCCNGGA | 1 cut(s) | 1591 |
| PkrI | GCNGC | 4 cut(s) | 612, 1012, 1015, 1278 |
| Ppu21I | YACGTR | 1 cut(s) | 200 |
| PscI | ACATGT | 1 cut(s) | 956 |
| PsiI | TTATAA | 1 cut(s) | 1509 |
| Psp6I | CCWGG | 5 cut(s) | 184, 340, 613, 805, 1591 |
| PspGI | CCWGG | 5 cut(s) | 184, 340, 613, 805, 1591 |
| PspN4I | GGNNCC | 2 cut(s) | 466, 571 |
| PspPI | GGNCC | 3 cut(s) | 85, 188, 308 |
| PstI | CTGCAG | 1 cut(s) | 898 |
| PstNI | CAGNNNCTG | 2 cut(s) | 902, 1307 |
| PsuI | RGATCY | 1 cut(s) | 1519 |
| RruI | TCGCGA | 1 cut(s) | 1695 |
| RsaI | GTAC | 6 cut(s) | 524, 571, 644, 998, 1061, 1122 |
| RsaNI | GTAC | 6 cut(s) | 523, 570, 643, 997, 1060, 1121 |
| RseI | CAYNNNNRTG | 7 cut(s) | 105, 210, 270, 588, 918, 1437, 1752 |
| SalI | GTCGAC | 1 cut(s) | 1193 |
| SaqAI | TTAA | 3 cut(s) | 602, 771, 1631 |
| SatI | GCNGC | 4 cut(s) | 611, 1011, 1014, 1277 |
| Sau3AI | GATC | 2 cut(s) | 1495, 1519 |
| Sau96I | GGNCC | 3 cut(s) | 85, 188, 308 |
| ScrFI | CCNGG | 5 cut(s) | 186, 342, 615, 807, 1593 |
| SduI | GDGCHC | 1 cut(s) | 1676 |
| SfaNI | GCATC | 2 cut(s) | 524, 580 |
| SfcI | CTRYAG | 2 cut(s) | 894, 1231 |
| SinI | GGWCC | 3 cut(s) | 85, 188, 308 |
| SmiMI | CAYNNNNRTG | 7 cut(s) | 105, 210, 270, 588, 918, 1437, 1752 |
| SmlI | CTYRAG | 1 cut(s) | 880 |
| SmoI | CTYRAG | 1 cut(s) | 880 |
| SphI | GCATGC | 2 cut(s) | 404, 633 |
| Sse9I | AATT | 8 cut(s) | 130, 510, 1261, 1380, 1477, 1510, 1735, 1760 |
| SsiI | CCGC | 4 cut(s) | 482, 1076, 1245, 1277 |
| SspI | AATATT | 2 cut(s) | 857, 1319 |
| SspMI | CTAG | 5 cut(s) | 420, 755, 830, 1092, 1686 |
| StyD4I | CCNGG | 5 cut(s) | 184, 340, 613, 805, 1591 |
| StyI | CCWWGG | 4 cut(s) | 241, 296, 1390, 1500 |
| TaaI | ACNGT | 9 cut(s) | 54, 220, 327, 391, 430, 647, 745, 1108, 1412 |
| TaiI | ACGT | 2 cut(s) | 202, 234 |
| TaqI | TCGA | 3 cut(s) | 1080, 1112, 1194 |
| TasI | AATT | 8 cut(s) | 130, 510, 1261, 1380, 1477, 1510, 1735, 1760 |
| TatI | WGTACW | 2 cut(s) | 996, 1120 |
| TauI | GCSGC | 1 cut(s) | 1279 |
| TfiI | GAWTC | 4 cut(s) | 1256, 1304, 1370, 1575 |
| Tru1I | TTAA | 3 cut(s) | 602, 771, 1631 |
| Tru9I | TTAA | 3 cut(s) | 602, 771, 1631 |
| TscAI | CASTG | 7 cut(s) | 272, 332, 748, 790, 898, 907, 1654 |
| TseFI | GTSAC | 2 cut(s) | 898, 1130 |
| TseI | GCWGC | 3 cut(s) | 610, 1010, 1013 |
| Tsp45I | GTSAC | 2 cut(s) | 898, 1130 |
| TspDTI | ATGAA | 8 cut(s) | 17, 375, 422, 507, 716, 1130, 1269, 1383 |
| TspGWI | ACGGA | 1 cut(s) | 708 |
| TspRI | CASTG | 7 cut(s) | 272, 332, 748, 790, 898, 907, 1654 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 267 |
| VpaK11BI | GGWCC | 3 cut(s) | 85, 188, 308 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1231 |
| XapI | RAATTY | 4 cut(s) | 1261, 1477, 1735, 1760 |
| XceI | RCATGY | 5 cut(s) | 21, 404, 633, 960, 1714 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 294 |
| XmiI | GTMKAC | 1 cut(s) | 1194 |
| XspI | CTAG | 5 cut(s) | 420, 755, 830, 1092, 1686 |
| Zsp2I | ATGCAT | 2 cut(s) | 1434, 1440 |