RchiOBHm_Chr6g0273631

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000320
Physical Location & Seq
Reverse (-)
34539662 .. 34556190
16529 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ24546

Sequence Viewer

Length: 1794 bp
ATGAAGCACCAAAAGACATGCCCATTGTCAACTACTGATCAGCTTCTGGTGTTTCTGTTTCTTATAGTGCAGTTCCTCTCCCTGCTGGTTCAAGCCGAAGTCCATTACTATGATTTTGTTGTAAGGGAGAAAAATTACACGAGGCTGTGTGAGACAAAGAGCATTTTAGTTGTGAATGATAGTTTCCCAGGACCGGAGATACGTGTTCACAAAGGTGATACAGTTTATGTCAACGTGCATAACCAAGGAGATTATGCATTCACCATTCACTGGCATGGGATAAAGCAACCACGAAATCCATGGTCAGATGGTCCATCTTACATCACACAGTGTCCAATCCCACCTGGAACAAACTTCACCTATGAAGTATTATTGTCCATAGAAGAAGGAACTGTATGGTGGCATGCACATAGTGATTGGACTAGAGCTACTGTTCATGGTGCTTTTGTTATCTTGCCTGCTATTGGAACCACATTCCCATTTCCGCAACCTGATGAAGACGAAGTTCTTGTAATTGCATCTTGGTACACAGGAGATATAAAAGAACTTATTGATGAGTCTATACAAAATGGTACCGACTTACCCCATTCAGATGCTTACACTTTAAATGGGCAGCCAGGAGATTTTTGTGCATGCTCCAATGGAACAGCGTACCGTCGTGTAGTGGATTACGGCAAGACATATCTTCTTCGTATAGTCAACGGAAACATGAATGCAGAACACTTCTTTGCCGTTGCAGAGCATAGTCTCACTGTGGTTGGACTAGATGGAGCCTACATAAAACCCATAAACACTGCCTACATAGTCATAAGTCCTGGACAAACGATGGATGTCTTGCTAGTAGCAAACCAGTCTCTTGGCCAATATTATATGGCTATTAGACAATACTCAAGTGAGAACATTGCAGTCACTAGATTTGACCATGCAAATGTTACTGCAATCCTTGAATACAGAGGCGACTATACATATGAGACATTTCCTGTATTTCCATCTACTCTTCCTATGTACTTGGACAAAGCAGCAGCACTCAACTTCACATACCAACTTAGAAGCTTGGCTACTCCAGAGTACCCTGTAAATGTTCCACTCGACATCACTACTAGAATGTATATTACGGCGTCGATGAATGTACTTCCTTGTGACCATGCAGGCTGTGAAATAACTGAGAATATCGCTTCAAGCCTAAATAATGTTAGTTGGGTCGACACAAAACCAACTACAAATGTCTTGGAAGCCTACTACAGGAACATAAGCGGGGCTTATGAATCAAATTTCCCAGACCAGCCGCCTTTGTTTTATAATTTTACAGCAGATTCTATCGCCGACTATTATGATTACACAGCACAAGGGACAAAGGTGAAAGTGTTGAACTATAATGAATCGGTTGAAATTGTGTTCCAAGGGACTAATGTGATGGACGGTTCTGTCAATCATCCAATGCATTTGCATGGATATAGCTTTTATGTGGTTGGATATGGTTTTGGAAATTTTGACAATGAGACTGATCCCAAGGGGTATAATTTGGTAGATCCTCCTAAAGTAACTACCTTTGGAGTTCCCAAAAGAGGATGGTTAGCAATCAGATTCAAAGCAACTAATCCTGGAGTGTGGTTTTGGCATTGTCATTTCGAGAGACACTTAACTTGGGGTATGGACACTGCTTTTATAGTGAAAAATGGGGGCACTCCGGAGACTAGCATTCGTGAGCCACCAACTTACATGCCCCCATGTATTATTCCATTGAATTCTCGTGTCCACAGCTATGGTGAATTTATTGAAGAGAAAATAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00053 ko01100 map00053 map01100
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

597

Amino Acids

67.49

Weight (kDa)

5.2

Isoelectric Point (pI)

32.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_3 PF07732 41 - 153 1.9e-40 Multicopper oxidase
Cu-oxidase PF00394 167 - 319 2.4e-34 Multicopper oxidase
Cu-oxidase_2 PF07731 429 - 559 4.6e-40 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000320)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g12640 FvH4_2g18980 FvH4_2g18990 FvH4_2g40270 FvH4_2g40270 FvH4_4g22740 FvH4_4g22740 FvH4_5g35760 FvH4_5g35760 FvH4_5g35760 FvH4_5g35760 FvH4_5g35760 FvH4_5g35760 FvH4_5g35760 FvH4_5g35760 FvH4_5g36460 FvH4_5g36460 FvH4_6g39950 FvH4_6g39970 FvH4_6g39983 FvH4_6g39990
rosa_chinensis RchiOBHm_Chr2g0154481 RchiOBHm_Chr2g0154511 RchiOBHm_Chr4g0428961 RchiOBHm_Chr6g0252431 RchiOBHm_Chr6g0273301 RchiOBHm_Chr6g0273631 RchiOBHm_Chr6g0273651 RchiOBHm_Chr6g0284751 RchiOBHm_Chr6g0284761 RchiOBHm_Chr6g0300491 RchiOBHm_Chr7g0237421 RchiOBHm_Chr7g0238581
rosa_laevigata RLG00000000906 RLG00000001006 RLG00000007110 RLG00000011333 RLG00000012721 RLG00000013605 RLG00000013626 RLG00000020780 RLG00000020782 RLG00000020783 RLG00000020786
rosa_multiflora Rmu_co8321753.1_g000001 Rmu_sc0000239.1_g000020 Rmu_sc0000686.1_g000001 Rmu_sc0000686.1_g000003 Rmu_sc0000686.1_g000005 Rmu_sc0001476.1_g000011 Rmu_sc0002231.1_g000002 Rmu_sc0002231.1_g000016 Rmu_sc0002690.1_g000003 Rmu_sc0002717.1_g000015 Rmu_sc0002923.1_g000026 Rmu_sc0003808.1_g000016 Rmu_sc0006475.1_g000008 Rmu_sc0006475.1_g000012 Rmu_sc0006475.1_g000018 Rmu_sc0014815.1_g000004 Rmu_sc0015313.1_g000012
rosa_roxburghii Rroxscaffold_2G00094270 Rroxscaffold_2G00094290 Rroxscaffold_3G00222950 Rroxscaffold_3G00224250 Rroxscaffold_5G00370630 Rroxscaffold_7G00167740 Rroxscaffold_7G00184410 Rroxscaffold_7G00195190 Rroxscaffold_7G00195460
rosa_rugosa Rorug02G0444600 Rorug04G0231100 Rorug04G0231200 Rorug06G0074000 Rorug06G0076800 Rorug06G0166400 Rorug06G0166500 Rorug06G0166600 Rorug06G0296400 Rorug07G0303000
rosa_samantha Rh2CG494600 Rh2CG494700 Rh2DG530800 Rh2DG530900 Rh2DG531000 Rh4AG287500 Rh4AG403800 Rh4BG293400 Rh4BG415100 Rh4DG290500 Rh6AG191000 Rh6AG191300 Rh6BG192500 Rh6BG194800 Rh6BG195200 Rh6BG279500 Rh6BG279600 Rh6DG183900 Rh6DG186600 Rh6DG274300 Rh6DG274500 Rh6DG409500 Rh7AG460600 Rh7AG468200 Rh7BG430200
rosa_wichuraiana Rw2G041820 Rw2G041830 Rw2G041840 Rw4G024930 Rw6G016470 Rw6G016720 Rw6G016730 Rw6G024020 Rw6G024030 Rw6G035790 Rw7G038130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1299
AasI GACNNNNNNGTC 1 cut(s) 1424
Acc65I GGTACC 1 cut(s) 572
AccB1I GGYRCC 1 cut(s) 572
AccB7I CCANNNNNTGG 1 cut(s) 270
AccI GTMKAC 1 cut(s) 1203
AccIII TCCGGA 1 cut(s) 1687
AciI CCGC 3 cut(s) 485, 1254, 1286
AclWI GGATC 2 cut(s) 1499, 1523
AcoI YGGCCR 1 cut(s) 859
AcsI RAATTY 4 cut(s) 1270, 1486, 1744, 1769
AcyI GRCGYC 1 cut(s) 1118
AdeI CACNNNGTG 2 cut(s) 330, 413
AfaI GTAC 6 cut(s) 527, 574, 653, 1007, 1070, 1131
AfiI CCNNNNNNNGG 5 cut(s) 193, 270, 464, 1242, 1565
AflIII ACRYGT 1 cut(s) 202
AgsI TTSAA 8 cut(s) 92, 947, 1179, 1369, 1388, 1588, 1744, 1778
AjnI CCWGG 5 cut(s) 187, 343, 616, 814, 1600
AleI CACNNNNGTG 1 cut(s) 213
AluBI AGCT 5 cut(s) 43, 428, 1053, 1458, 1761
AluI AGCT 5 cut(s) 43, 428, 1053, 1458, 1761
Alw26I GTCTC 7 cut(s) 146, 752, 858, 965, 1493, 1627, 1685
AlwI GGATC 2 cut(s) 1499, 1523
AlwNI CAGNNNCTG 1 cut(s) 46
Aor13HI TCCGGA 1 cut(s) 1687
AoxI GGCC 1 cut(s) 859
ApeKI GCWGC 3 cut(s) 613, 1019, 1022
ApoI RAATTY 4 cut(s) 1270, 1486, 1744, 1769
Asp718I GGTACC 1 cut(s) 572
AspS9I GGNCC 2 cut(s) 191, 311
AsuHPI GGTGA 5 cut(s) 227, 253, 349, 1369, 1778
AvaII GGWCC 2 cut(s) 191, 311
BaeGI GKGCMC 1 cut(s) 1685
BalI TGGCCA 1 cut(s) 861
BanI GGYRCC 1 cut(s) 572
BarI GAAGNNNNNNTAC 2 cut(s) 1042, 1074
BauI CACGAG 2 cut(s) 139, 1749
BbsI GAAGAC 1 cut(s) 504
BbvI GCAGC 3 cut(s) 625, 1031, 1034
BccI CCATC 7 cut(s) 302, 322, 761, 820, 997, 1408, 1563
BceAI ACGGC 3 cut(s) 688, 716, 1131
BciT130I CCWGG 5 cut(s) 189, 345, 618, 816, 1602
BclI TGATCA 1 cut(s) 37
BcoDI GTCTC 7 cut(s) 146, 752, 858, 965, 1493, 1627, 1685
BfaI CTAG 6 cut(s) 423, 764, 839, 912, 1101, 1695
BfmI CTRYAG 1 cut(s) 1240
BisI GCNGC 4 cut(s) 614, 1020, 1023, 1286
BlsI GCNGC 4 cut(s) 615, 1021, 1024, 1287
Bme1390I CCNGG 5 cut(s) 189, 345, 618, 816, 1602
Bme18I GGWCC 2 cut(s) 191, 311
BmgT120I GGNCC 2 cut(s) 191, 311
BmiI GGNNCC 3 cut(s) 469, 574, 772
BmrFI CCNGG 5 cut(s) 189, 345, 618, 816, 1602
BmsI GCATC 2 cut(s) 527, 583
BpiI GAAGAC 1 cut(s) 504
BpmI CTGGAG 2 cut(s) 1047, 1623
BpuEI CTTGAG 1 cut(s) 874
BsaAI YACGTR 1 cut(s) 203
BsaHI GRCGYC 1 cut(s) 1118
BsaJI CCNNGG 5 cut(s) 187, 244, 299, 1399, 1509
BsaWI WCCGGW 2 cut(s) 193, 1687
Bsc4I CCNNNNNNNGG 5 cut(s) 193, 270, 464, 1242, 1565
Bse1I ACTGG 2 cut(s) 275, 850
Bse3DI GCAATG 1 cut(s) 900
BseAI TCCGGA 1 cut(s) 1687
BseBI CCWGG 5 cut(s) 189, 345, 618, 816, 1602
BseDI CCNNGG 5 cut(s) 187, 244, 299, 1399, 1509
BseGI GGATG 3 cut(s) 835, 1432, 1574
BseLI CCNNNNNNNGG 5 cut(s) 193, 270, 464, 1242, 1565
BseMI GCAATG 1 cut(s) 900
BseMII CTCAG 1 cut(s) 1155
BseNI ACTGG 2 cut(s) 275, 850
BseSI GKGCMC 1 cut(s) 1685
BseXI GCAGC 3 cut(s) 625, 1031, 1034
BsgI GTGCAG 1 cut(s) 89
BshFI GGCC 1 cut(s) 861
BshNI GGYRCC 1 cut(s) 572
BsiSI CCGG 2 cut(s) 194, 1688
BslFI GGGAC 2 cut(s) 1363, 1417
BslI CCNNNNNNNGG 5 cut(s) 193, 270, 464, 1242, 1565
BsmAI GTCTC 7 cut(s) 146, 752, 858, 965, 1493, 1627, 1685
BsmFI GGGAC 2 cut(s) 1363, 1417
BsmI GAATGC 3 cut(s) 257, 718, 1698
BsnI GGCC 1 cut(s) 861
Bsp1286I GDGCHC 1 cut(s) 1685
Bsp13I TCCGGA 1 cut(s) 1687
Bsp143I GATC 3 cut(s) 37, 1504, 1528
Bsp19I CCATGG 1 cut(s) 299
BspACI CCGC 3 cut(s) 485, 1254, 1286
BspANI GGCC 1 cut(s) 861
BspCNI CTCAG 1 cut(s) 1156
BspEI TCCGGA 1 cut(s) 1687
BspLI GGNNCC 3 cut(s) 469, 574, 772
BspPI GGATC 2 cut(s) 1499, 1523
BspT107I GGYRCC 1 cut(s) 572
BsrDI GCAATG 1 cut(s) 900
BsrI ACTGG 2 cut(s) 275, 850
BssECI CCNNGG 5 cut(s) 187, 244, 299, 1399, 1509
BssMI GATC 3 cut(s) 37, 1504, 1528
BssNI GRCGYC 1 cut(s) 1118
BssSI CACGAG 2 cut(s) 139, 1749
BssT1I CCWWGG 4 cut(s) 244, 299, 1399, 1509
Bst2BI CACGAG 2 cut(s) 139, 1749
Bst2UI CCWGG 5 cut(s) 189, 345, 618, 816, 1602
Bst4CI ACNGT 7 cut(s) 223, 330, 394, 433, 656, 754, 1421
Bst6I CTCTTC 2 cut(s) 1002, 1773
BstACI GRCGYC 1 cut(s) 1118
BstBAI YACGTR 1 cut(s) 203
BstC8I GCNNGC 4 cut(s) 405, 459, 634, 1150
BstDEI CTNAG 2 cut(s) 1046, 1164
BstDSI CCRYGG 1 cut(s) 299
BstENI CCTNNNNNAGG 1 cut(s) 1240
BstF5I GGATG 3 cut(s) 835, 1432, 1574
BstKTI GATC 3 cut(s) 40, 1507, 1531
BstMAI GTCTC 7 cut(s) 146, 752, 858, 965, 1493, 1627, 1685
BstMBI GATC 3 cut(s) 37, 1504, 1528
BstNI CCWGG 5 cut(s) 189, 345, 618, 816, 1602
BstNSI RCATGY 4 cut(s) 21, 407, 636, 1723
BstSCI CCNGG 5 cut(s) 187, 343, 616, 814, 1600
BstSFI CTRYAG 1 cut(s) 1240
BstSLI GKGCMC 1 cut(s) 1685
BstV1I GCAGC 3 cut(s) 625, 1031, 1034
BstV2I GAAGAC 1 cut(s) 504
BstX2I RGATCY 1 cut(s) 1528
BstXI CCANNNNNNTGG 2 cut(s) 857, 1763
BstYI RGATCY 1 cut(s) 1528
BsuRI GGCC 1 cut(s) 861
BtgI CCRYGG 1 cut(s) 299
BtsCI GGATG 3 cut(s) 835, 1432, 1574
BtsI GCAGTG 2 cut(s) 792, 1656
BtsIMutI CAGTG 5 cut(s) 268, 335, 750, 792, 1656
Cac8I GCNNGC 4 cut(s) 405, 459, 634, 1150
CaiI CAGNNNCTG 1 cut(s) 46
Cfr13I GGNCC 2 cut(s) 191, 311
CseI GACGC 1 cut(s) 1107
Csp6I GTAC 6 cut(s) 526, 573, 652, 1006, 1069, 1130
CviQI GTAC 6 cut(s) 526, 573, 652, 1006, 1069, 1130
DdeI CTNAG 2 cut(s) 1046, 1164
DpnI GATC 3 cut(s) 39, 1506, 1530
DpnII GATC 3 cut(s) 37, 1504, 1528
DraI TTTAAA 1 cut(s) 606
DraIII CACNNNGTG 2 cut(s) 330, 413
DrdI GACNNNNNNGTC 1 cut(s) 1424
DseDI GACNNNNNNGTC 1 cut(s) 1424
EaeI YGGCCR 1 cut(s) 859
Eam1104I CTCTTC 2 cut(s) 1002, 1773
EarI CTCTTC 2 cut(s) 1002, 1773
Eco130I CCWWGG 4 cut(s) 244, 299, 1399, 1509
Eco47I GGWCC 2 cut(s) 191, 311
EcoNI CCTNNNNNAGG 1 cut(s) 1240
EcoRI GAATTC 1 cut(s) 1744
EcoRII CCWGG 5 cut(s) 187, 343, 616, 814, 1600
EcoT14I CCWWGG 4 cut(s) 244, 299, 1399, 1509
EcoT22I ATGCAT 2 cut(s) 259, 1443
ErhI CCWWGG 4 cut(s) 244, 299, 1399, 1509
FalI AAGNNNNNCTT 2 cut(s) 1243, 1275
FaqI GGGAC 2 cut(s) 1363, 1417
FauI CCCGC 1 cut(s) 1247
FauNDI CATATG 1 cut(s) 967
FbaI TGATCA 1 cut(s) 37
FblI GTMKAC 1 cut(s) 1203
Fnu4HI GCNGC 4 cut(s) 614, 1020, 1023, 1286
FokI GGATG 3 cut(s) 842, 1419, 1581
Fsp4HI GCNGC 4 cut(s) 614, 1020, 1023, 1286
FspBI CTAG 6 cut(s) 423, 764, 839, 912, 1101, 1695
GluI GCNGC 4 cut(s) 614, 1020, 1023, 1286
GsuI CTGGAG 2 cut(s) 1047, 1623
HaeIII GGCC 1 cut(s) 861
HapII CCGG 2 cut(s) 194, 1688
HgaI GACGC 1 cut(s) 1107
Hin1I GRCGYC 1 cut(s) 1118
HincII GTYRAC 4 cut(s) 30, 232, 700, 1204
HindII GTYRAC 4 cut(s) 30, 232, 700, 1204
HindIII AAGCTT 1 cut(s) 1051
HinfI GANTC 5 cut(s) 557, 1265, 1313, 1379, 1584
HpaII CCGG 2 cut(s) 194, 1688
HphI GGTGA 5 cut(s) 227, 253, 349, 1369, 1778
Hpy166II GTNNAC 7 cut(s) 30, 208, 232, 528, 700, 1204, 1756
Hpy188I TCNGA 3 cut(s) 307, 592, 1583
Hpy188III TCNNGA 4 cut(s) 1064, 1630, 1688, 1703
Hpy8I GTNNAC 7 cut(s) 30, 208, 232, 528, 700, 1204, 1756
Hpy99I CGWCG 2 cut(s) 660, 1123
HpyAV CCTTC 1 cut(s) 380
HpyCH4III ACNGT 7 cut(s) 223, 330, 394, 433, 656, 754, 1421
HpyCH4IV ACGT 2 cut(s) 202, 234
HpyF3I CTNAG 2 cut(s) 1046, 1164
HpySE526I ACGT 2 cut(s) 202, 234
Hsp92I GRCGYC 1 cut(s) 1118
Kpn2I TCCGGA 1 cut(s) 1687
KpnI GGTACC 1 cut(s) 576
Ksp22I TGATCA 1 cut(s) 37
Kzo9I GATC 3 cut(s) 37, 1504, 1528
LmnI GCTCC 2 cut(s) 641, 770
Lsp1109I GCAGC 3 cut(s) 625, 1031, 1034
LweI GCATC 2 cut(s) 527, 583
MaeI CTAG 6 cut(s) 423, 764, 839, 912, 1101, 1695
MaeII ACGT 2 cut(s) 202, 234
MaeIII GTNAC 4 cut(s) 907, 931, 1139, 1540
MalI GATC 3 cut(s) 39, 1506, 1530
MboI GATC 3 cut(s) 37, 1504, 1528
MboII GAAGA 6 cut(s) 395, 509, 677, 680, 989, 1790
MflI RGATCY 1 cut(s) 1528
MhlI GDGCHC 1 cut(s) 1685
MlsI TGGCCA 1 cut(s) 861
MluCI AATT 9 cut(s) 133, 513, 1270, 1300, 1389, 1486, 1519, 1744, 1769
MluNI TGGCCA 1 cut(s) 861
MlyI GAGTC 1 cut(s) 566
MmeI TCCRAC 2 cut(s) 739, 1450
MnlI CCTC 5 cut(s) 86, 135, 947, 1542, 1559
Mox20I TGGCCA 1 cut(s) 861
Mph1103I ATGCAT 2 cut(s) 259, 1443
MroI TCCGGA 1 cut(s) 1687
MscI TGGCCA 1 cut(s) 861
MseI TTAA 2 cut(s) 605, 1640
MslI CAYNNNNRTG 7 cut(s) 108, 213, 273, 591, 927, 1446, 1761
Msp20I TGGCCA 1 cut(s) 861
MspI CCGG 2 cut(s) 194, 1688
MspR9I CCNGG 5 cut(s) 189, 345, 618, 816, 1602
Mva1269I GAATGC 3 cut(s) 257, 718, 1698
MvaI CCWGG 5 cut(s) 189, 345, 618, 816, 1602
NcoI CCATGG 1 cut(s) 299
NdeI CATATG 1 cut(s) 967
NdeII GATC 3 cut(s) 37, 1504, 1528
NlaIV GGNNCC 3 cut(s) 469, 574, 772
NmuCI GTSAC 2 cut(s) 907, 1139
NsiI ATGCAT 2 cut(s) 259, 1443
NspI RCATGY 4 cut(s) 21, 407, 636, 1723
OliI CACNNNNGTG 1 cut(s) 213
PaeI GCATGC 2 cut(s) 407, 636
PctI GAATGC 3 cut(s) 257, 718, 1698
PfeI GAWTC 4 cut(s) 1265, 1313, 1379, 1584
PflMI CCANNNNNTGG 1 cut(s) 270
PfoI TCCNGGA 2 cut(s) 814, 1600
PkrI GCNGC 4 cut(s) 615, 1021, 1024, 1287
PleI GAGTC 1 cut(s) 565
PpsI GAGTC 1 cut(s) 565
Ppu21I YACGTR 1 cut(s) 203
PsiI TTATAA 1 cut(s) 1299
Psp6I CCWGG 5 cut(s) 187, 343, 616, 814, 1600
PspGI CCWGG 5 cut(s) 187, 343, 616, 814, 1600
PspN4I GGNNCC 3 cut(s) 469, 574, 772
PspPI GGNCC 2 cut(s) 191, 311
PstNI CAGNNNCTG 1 cut(s) 46
PsuI RGATCY 1 cut(s) 1528
RsaI GTAC 6 cut(s) 527, 574, 653, 1007, 1070, 1131
RsaNI GTAC 6 cut(s) 526, 573, 652, 1006, 1069, 1130
RseI CAYNNNNRTG 7 cut(s) 108, 213, 273, 591, 927, 1446, 1761
SalI GTCGAC 1 cut(s) 1202
SaqAI TTAA 2 cut(s) 605, 1640
SatI GCNGC 4 cut(s) 614, 1020, 1023, 1286
Sau3AI GATC 3 cut(s) 37, 1504, 1528
Sau96I GGNCC 2 cut(s) 191, 311
SchI GAGTC 1 cut(s) 566
ScrFI CCNGG 5 cut(s) 189, 345, 618, 816, 1602
SduI GDGCHC 1 cut(s) 1685
SfaNI GCATC 2 cut(s) 527, 583
SfcI CTRYAG 1 cut(s) 1240
SinI GGWCC 2 cut(s) 191, 311
SmiMI CAYNNNNRTG 7 cut(s) 108, 213, 273, 591, 927, 1446, 1761
SmlI CTYRAG 1 cut(s) 889
SmoI CTYRAG 1 cut(s) 889
SphI GCATGC 2 cut(s) 407, 636
Sse9I AATT 9 cut(s) 133, 513, 1270, 1300, 1389, 1486, 1519, 1744, 1769
SsiI CCGC 3 cut(s) 485, 1254, 1286
SspI AATATT 1 cut(s) 866
SspMI CTAG 6 cut(s) 423, 764, 839, 912, 1101, 1695
StyD4I CCNGG 5 cut(s) 187, 343, 616, 814, 1600
StyI CCWWGG 4 cut(s) 244, 299, 1399, 1509
TaaI ACNGT 7 cut(s) 223, 330, 394, 433, 656, 754, 1421
TaiI ACGT 2 cut(s) 205, 237
TaqI TCGA 4 cut(s) 1089, 1121, 1203, 1629
TasI AATT 9 cut(s) 133, 513, 1270, 1300, 1389, 1486, 1519, 1744, 1769
TatI WGTACW 2 cut(s) 1005, 1129
TauI GCSGC 1 cut(s) 1288
TfiI GAWTC 4 cut(s) 1265, 1313, 1379, 1584
Tru1I TTAA 2 cut(s) 605, 1640
Tru9I TTAA 2 cut(s) 605, 1640
TscAI CASTG 5 cut(s) 275, 335, 757, 799, 1663
TseFI GTSAC 2 cut(s) 907, 1139
TseI GCWGC 3 cut(s) 613, 1019, 1022
Tsp45I GTSAC 2 cut(s) 907, 1139
TspDTI ATGAA 8 cut(s) 17, 378, 425, 510, 725, 1139, 1278, 1392
TspGWI ACGGA 1 cut(s) 717
TspRI CASTG 5 cut(s) 275, 335, 757, 799, 1663
Van91I CCANNNNNTGG 1 cut(s) 270
VpaK11BI GGWCC 2 cut(s) 191, 311
XagI CCTNNNNNAGG 1 cut(s) 1240
XapI RAATTY 4 cut(s) 1270, 1486, 1744, 1769
XceI RCATGY 4 cut(s) 21, 407, 636, 1723
XcmI CCANNNNNNNNNTGG 1 cut(s) 297
XmiI GTMKAC 1 cut(s) 1203
XspI CTAG 6 cut(s) 423, 764, 839, 912, 1101, 1695
Zsp2I ATGCAT 2 cut(s) 259, 1443
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.