Lignin degradation and detoxification of lignin-derived products
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1190 |
| AccB1I | GGYRCC | 1 cut(s) | 512 |
| AccI | GTMKAC | 1 cut(s) | 952 |
| AccII | CGCG | 1 cut(s) | 496 |
| AclWI | GGATC | 1 cut(s) | 702 |
| AcoI | YGGCCR | 1 cut(s) | 682 |
| AcsI | RAATTY | 3 cut(s) | 49, 986, 1204 |
| AcyI | GRCGYC | 1 cut(s) | 513 |
| AdeI | CACNNNGTG | 1 cut(s) | 402 |
| AfaI | GTAC | 4 cut(s) | 59, 174, 599, 1079 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 265, 536, 691 |
| AgsI | TTSAA | 3 cut(s) | 54, 164, 613 |
| AhlI | ACTAGT | 1 cut(s) | 1367 |
| AjnI | CCWGG | 5 cut(s) | 259, 415, 593, 688, 886 |
| AluBI | AGCT | 5 cut(s) | 1125, 1196, 1239, 1281, 1316 |
| AluI | AGCT | 5 cut(s) | 1125, 1196, 1239, 1281, 1316 |
| Alw21I | GWGCWC | 1 cut(s) | 1301 |
| Alw26I | GTCTC | 2 cut(s) | 335, 710 |
| AlwI | GGATC | 1 cut(s) | 702 |
| Ama87I | CYCGRG | 1 cut(s) | 1291 |
| AoxI | GGCC | 2 cut(s) | 263, 682 |
| ApeKI | GCWGC | 2 cut(s) | 1091, 1239 |
| ApoI | RAATTY | 3 cut(s) | 49, 986, 1204 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 206 |
| AspLEI | GCGC | 2 cut(s) | 515, 936 |
| AspS9I | GGNCC | 2 cut(s) | 263, 383 |
| AsuC2I | CCSGG | 1 cut(s) | 214 |
| AsuHPI | GGTGA | 2 cut(s) | 202, 1297 |
| AvaI | CYCGRG | 1 cut(s) | 1291 |
| AvaII | GGWCC | 1 cut(s) | 383 |
| BalI | TGGCCA | 1 cut(s) | 684 |
| BanI | GGYRCC | 1 cut(s) | 512 |
| Bbv12I | GWGCWC | 1 cut(s) | 1301 |
| BbvI | GCAGC | 2 cut(s) | 1103, 1226 |
| BccI | CCATC | 2 cut(s) | 100, 374 |
| BciT130I | CCWGG | 5 cut(s) | 261, 417, 595, 690, 888 |
| BciVI | GTATCC | 1 cut(s) | 283 |
| BcnI | CCSGG | 1 cut(s) | 214 |
| BcoDI | GTCTC | 2 cut(s) | 335, 710 |
| BcuI | ACTAGT | 1 cut(s) | 1367 |
| BfaI | CTAG | 5 cut(s) | 116, 836, 963, 1368, 1378 |
| BfmI | CTRYAG | 2 cut(s) | 137, 1321 |
| BfoI | RGCGCY | 2 cut(s) | 516, 937 |
| BfuI | GTATCC | 1 cut(s) | 283 |
| BglII | AGATCT | 1 cut(s) | 1303 |
| BisI | GCNGC | 2 cut(s) | 1092, 1240 |
| BlpI | GCTNAGC | 1 cut(s) | 1126 |
| BlsI | GCNGC | 2 cut(s) | 1093, 1241 |
| BmcAI | AGTACT | 1 cut(s) | 59 |
| Bme1390I | CCNGG | 6 cut(s) | 214, 261, 417, 595, 690, 888 |
| Bme18I | GGWCC | 1 cut(s) | 383 |
| BmeT110I | CYCGRG | 1 cut(s) | 1291 |
| BmgT120I | GGNCC | 2 cut(s) | 263, 383 |
| BmiI | GGNNCC | 3 cut(s) | 514, 844, 1231 |
| BmrFI | CCNGG | 6 cut(s) | 214, 261, 417, 595, 690, 888 |
| BmsI | GCATC | 3 cut(s) | 655, 775, 1103 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1126 |
| BpuEI | CTTGAG | 1 cut(s) | 1255 |
| BpuMI | CCSGG | 1 cut(s) | 214 |
| BsaHI | GRCGYC | 1 cut(s) | 513 |
| BsaI | GGTCTC | 1 cut(s) | 335 |
| BsaJI | CCNNGG | 7 cut(s) | 259, 260, 371, 416, 689, 886, 1292 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 265, 536, 691 |
| Bse1I | ACTGG | 2 cut(s) | 347, 494 |
| BseBI | CCWGG | 5 cut(s) | 261, 417, 595, 690, 888 |
| BseDI | CCNNGG | 7 cut(s) | 259, 260, 371, 416, 689, 886, 1292 |
| BseGI | GGATG | 3 cut(s) | 590, 873, 907 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 265, 536, 691 |
| BseMII | CTCAG | 1 cut(s) | 378 |
| BseNI | ACTGG | 2 cut(s) | 347, 494 |
| BseRI | GAGGAG | 2 cut(s) | 1041, 1309 |
| BseXI | GCAGC | 2 cut(s) | 1103, 1226 |
| BseYI | CCCAGC | 1 cut(s) | 1281 |
| Bsh1236I | CGCG | 1 cut(s) | 496 |
| BshFI | GGCC | 2 cut(s) | 265, 684 |
| BshNI | GGYRCC | 1 cut(s) | 512 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1301 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1291 |
| BsiSI | CCGG | 2 cut(s) | 213, 266 |
| BslFI | GGGAC | 2 cut(s) | 433, 1139 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 265, 536, 691 |
| BsmAI | GTCTC | 2 cut(s) | 335, 710 |
| BsmFI | GGGAC | 2 cut(s) | 433, 1139 |
| BsnI | GGCC | 2 cut(s) | 265, 684 |
| Bso31I | GGTCTC | 1 cut(s) | 335 |
| BsoBI | CYCGRG | 1 cut(s) | 1291 |
| Bsp1286I | GDGCHC | 1 cut(s) | 1301 |
| Bsp143I | GATC | 4 cut(s) | 150, 694, 919, 1303 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1126 |
| Bsp19I | CCATGG | 1 cut(s) | 371 |
| BspANI | GGCC | 2 cut(s) | 265, 684 |
| BspCNI | CTCAG | 1 cut(s) | 379 |
| BspFNI | CGCG | 1 cut(s) | 496 |
| BspLI | GGNNCC | 3 cut(s) | 514, 844, 1231 |
| BspMAI | CTGCAG | 1 cut(s) | 141 |
| BspPI | GGATC | 1 cut(s) | 702 |
| BspT107I | GGYRCC | 1 cut(s) | 512 |
| BspTNI | GGTCTC | 1 cut(s) | 335 |
| BsrI | ACTGG | 2 cut(s) | 347, 494 |
| BssECI | CCNNGG | 7 cut(s) | 259, 260, 371, 416, 689, 886, 1292 |
| BssMI | GATC | 4 cut(s) | 150, 694, 919, 1303 |
| BssNI | GRCGYC | 1 cut(s) | 513 |
| BssT1I | CCWWGG | 1 cut(s) | 371 |
| Bst2UI | CCWGG | 5 cut(s) | 261, 417, 595, 690, 888 |
| Bst4CI | ACNGT | 7 cut(s) | 22, 295, 402, 466, 535, 826, 1217 |
| BstACI | GRCGYC | 1 cut(s) | 513 |
| BstC8I | GCNNGC | 5 cut(s) | 477, 498, 502, 686, 1314 |
| BstDEI | CTNAG | 3 cut(s) | 387, 1081, 1126 |
| BstDSI | CCRYGG | 1 cut(s) | 371 |
| BstF5I | GGATG | 3 cut(s) | 590, 873, 907 |
| BstFNI | CGCG | 1 cut(s) | 496 |
| BstH2I | RGCGCY | 2 cut(s) | 516, 937 |
| BstHHI | GCGC | 2 cut(s) | 515, 936 |
| BstKTI | GATC | 4 cut(s) | 153, 697, 922, 1306 |
| BstMAI | GTCTC | 2 cut(s) | 335, 710 |
| BstMBI | GATC | 4 cut(s) | 150, 694, 919, 1303 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 510, 1236 |
| BstNI | CCWGG | 5 cut(s) | 261, 417, 595, 690, 888 |
| BstNSI | RCATGY | 2 cut(s) | 238, 479 |
| BstSCI | CCNGG | 6 cut(s) | 212, 259, 415, 593, 688, 886 |
| BstSFI | CTRYAG | 2 cut(s) | 137, 1321 |
| BstUI | CGCG | 1 cut(s) | 496 |
| BstV1I | GCAGC | 2 cut(s) | 1103, 1226 |
| BstX2I | RGATCY | 2 cut(s) | 694, 1303 |
| BstYI | RGATCY | 2 cut(s) | 694, 1303 |
| BsuI | GTATCC | 1 cut(s) | 283 |
| BsuRI | GGCC | 2 cut(s) | 265, 684 |
| BtgI | CCRYGG | 1 cut(s) | 371 |
| BtsCI | GGATG | 3 cut(s) | 590, 873, 907 |
| BtsIMutI | CAGTG | 3 cut(s) | 340, 407, 822 |
| Cac8I | GCNNGC | 5 cut(s) | 477, 498, 502, 686, 1314 |
| CfoI | GCGC | 2 cut(s) | 515, 936 |
| Cfr13I | GGNCC | 2 cut(s) | 263, 383 |
| CseI | GACGC | 1 cut(s) | 502 |
| Csp6I | GTAC | 4 cut(s) | 58, 173, 598, 1078 |
| CviQI | GTAC | 4 cut(s) | 58, 173, 598, 1078 |
| DdeI | CTNAG | 3 cut(s) | 387, 1081, 1126 |
| DinI | GGCGCC | 1 cut(s) | 514 |
| DpnI | GATC | 4 cut(s) | 152, 696, 921, 1305 |
| DpnII | GATC | 4 cut(s) | 150, 694, 919, 1303 |
| DraIII | CACNNNGTG | 1 cut(s) | 402 |
| EaeI | YGGCCR | 1 cut(s) | 682 |
| Eco130I | CCWWGG | 1 cut(s) | 371 |
| Eco31I | GGTCTC | 1 cut(s) | 335 |
| Eco47I | GGWCC | 1 cut(s) | 383 |
| Eco88I | CYCGRG | 1 cut(s) | 1291 |
| EcoRI | GAATTC | 1 cut(s) | 1204 |
| EcoRII | CCWGG | 5 cut(s) | 259, 415, 593, 688, 886 |
| EcoT14I | CCWWGG | 1 cut(s) | 371 |
| EcoT22I | ATGCAT | 2 cut(s) | 670, 790 |
| EgeI | GGCGCC | 1 cut(s) | 514 |
| EheI | GGCGCC | 1 cut(s) | 514 |
| ErhI | CCWWGG | 1 cut(s) | 371 |
| FaqI | GGGAC | 2 cut(s) | 433, 1139 |
| FblI | GTMKAC | 1 cut(s) | 952 |
| Fnu4HI | GCNGC | 2 cut(s) | 1092, 1240 |
| FokI | GGATG | 3 cut(s) | 577, 860, 914 |
| Fsp4HI | GCNGC | 2 cut(s) | 1092, 1240 |
| FspBI | CTAG | 5 cut(s) | 116, 836, 963, 1368, 1378 |
| GlaI | GCGC | 2 cut(s) | 514, 935 |
| GluI | GCNGC | 2 cut(s) | 1092, 1240 |
| GsaI | CCCAGC | 1 cut(s) | 1285 |
| HaeII | RGCGCY | 2 cut(s) | 516, 937 |
| HaeIII | GGCC | 2 cut(s) | 265, 684 |
| HapII | CCGG | 2 cut(s) | 213, 266 |
| HgaI | GACGC | 1 cut(s) | 502 |
| HhaI | GCGC | 2 cut(s) | 515, 936 |
| Hin1I | GRCGYC | 1 cut(s) | 513 |
| Hin6I | GCGC | 2 cut(s) | 513, 934 |
| HinP1I | GCGC | 2 cut(s) | 513, 934 |
| HindIII | AAGCTT | 2 cut(s) | 1123, 1314 |
| HinfI | GANTC | 1 cut(s) | 1223 |
| HpaII | CCGG | 2 cut(s) | 213, 266 |
| HphI | GGTGA | 2 cut(s) | 202, 1297 |
| Hpy166II | GTNNAC | 3 cut(s) | 953, 1288, 1373 |
| Hpy188I | TCNGA | 3 cut(s) | 379, 664, 985 |
| Hpy188III | TCNNGA | 2 cut(s) | 145, 386 |
| Hpy8I | GTNNAC | 3 cut(s) | 953, 1288, 1373 |
| HpyAV | CCTTC | 2 cut(s) | 452, 1079 |
| HpyCH4III | ACNGT | 7 cut(s) | 22, 295, 402, 466, 535, 826, 1217 |
| HpyCH4IV | ACGT | 2 cut(s) | 81, 752 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 510, 1236 |
| HpyF3I | CTNAG | 3 cut(s) | 387, 1081, 1126 |
| HpySE526I | ACGT | 2 cut(s) | 81, 752 |
| Hsp92I | GRCGYC | 1 cut(s) | 513 |
| HspAI | GCGC | 2 cut(s) | 513, 934 |
| KasI | GGCGCC | 1 cut(s) | 512 |
| Kzo9I | GATC | 4 cut(s) | 150, 694, 919, 1303 |
| LmnI | GCTCC | 4 cut(s) | 713, 842, 1235, 1296 |
| Lsp1109I | GCAGC | 2 cut(s) | 1103, 1226 |
| LweI | GCATC | 3 cut(s) | 655, 775, 1103 |
| MaeI | CTAG | 5 cut(s) | 116, 836, 963, 1368, 1378 |
| MaeII | ACGT | 2 cut(s) | 81, 752 |
| MaeIII | GTNAC | 2 cut(s) | 485, 1003 |
| MalI | GATC | 4 cut(s) | 152, 696, 921, 1305 |
| MboI | GATC | 4 cut(s) | 150, 694, 919, 1303 |
| MboII | GAAGA | 8 cut(s) | 115, 467, 581, 650, 749, 752, 790, 1232 |
| MflI | RGATCY | 2 cut(s) | 694, 1303 |
| MhlI | GDGCHC | 1 cut(s) | 1301 |
| MlsI | TGGCCA | 1 cut(s) | 684 |
| MluCI | AATT | 6 cut(s) | 49, 359, 424, 986, 1116, 1204 |
| MluNI | TGGCCA | 1 cut(s) | 684 |
| Mly113I | GGCGCC | 1 cut(s) | 513 |
| MmeI | TCCRAC | 5 cut(s) | 436, 517, 735, 811, 1067 |
| MnlI | CCTC | 8 cut(s) | 567, 568, 622, 830, 887, 1062, 1141, 1287 |
| Mox20I | TGGCCA | 1 cut(s) | 684 |
| Mph1103I | ATGCAT | 2 cut(s) | 670, 790 |
| MroXI | GAANNNNTTC | 1 cut(s) | 206 |
| MscI | TGGCCA | 1 cut(s) | 684 |
| MseI | TTAA | 3 cut(s) | 362, 1100, 1119 |
| MslI | CAYNNNNRTG | 4 cut(s) | 180, 345, 663, 999 |
| Msp20I | TGGCCA | 1 cut(s) | 684 |
| MspI | CCGG | 2 cut(s) | 213, 266 |
| MspR9I | CCNGG | 6 cut(s) | 214, 261, 417, 595, 690, 888 |
| MvaI | CCWGG | 5 cut(s) | 261, 417, 595, 690, 888 |
| MvnI | CGCG | 1 cut(s) | 496 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 510, 1236 |
| NarI | GGCGCC | 1 cut(s) | 513 |
| NciI | CCSGG | 1 cut(s) | 214 |
| NcoI | CCATGG | 1 cut(s) | 371 |
| NdeII | GATC | 4 cut(s) | 150, 694, 919, 1303 |
| NlaIV | GGNNCC | 3 cut(s) | 514, 844, 1231 |
| NmuCI | GTSAC | 1 cut(s) | 485 |
| NsiI | ATGCAT | 2 cut(s) | 670, 790 |
| NspI | RCATGY | 2 cut(s) | 238, 479 |
| PaeI | GCATGC | 1 cut(s) | 479 |
| PasI | CCCWGGG | 1 cut(s) | 260 |
| PdmI | GAANNNNTTC | 1 cut(s) | 206 |
| PfeI | GAWTC | 1 cut(s) | 1223 |
| PflFI | GACNNNGTC | 1 cut(s) | 995 |
| PkrI | GCNGC | 2 cut(s) | 1093, 1241 |
| PluTI | GGCGCC | 1 cut(s) | 516 |
| PsiI | TTATAA | 1 cut(s) | 1190 |
| Psp6I | CCWGG | 5 cut(s) | 259, 415, 593, 688, 886 |
| PspFI | CCCAGC | 1 cut(s) | 1281 |
| PspGI | CCWGG | 5 cut(s) | 259, 415, 593, 688, 886 |
| PspN4I | GGNNCC | 3 cut(s) | 514, 844, 1231 |
| PspPI | GGNCC | 2 cut(s) | 263, 383 |
| PstI | CTGCAG | 1 cut(s) | 141 |
| PsuI | RGATCY | 2 cut(s) | 694, 1303 |
| PsyI | GACNNNGTC | 1 cut(s) | 995 |
| RsaI | GTAC | 4 cut(s) | 59, 174, 599, 1079 |
| RsaNI | GTAC | 4 cut(s) | 58, 173, 598, 1078 |
| RseI | CAYNNNNRTG | 4 cut(s) | 180, 345, 663, 999 |
| SaqAI | TTAA | 3 cut(s) | 362, 1100, 1119 |
| SatI | GCNGC | 2 cut(s) | 1092, 1240 |
| Sau3AI | GATC | 4 cut(s) | 150, 694, 919, 1303 |
| Sau96I | GGNCC | 2 cut(s) | 263, 383 |
| ScaI | AGTACT | 1 cut(s) | 59 |
| ScrFI | CCNGG | 6 cut(s) | 214, 261, 417, 595, 690, 888 |
| SduI | GDGCHC | 1 cut(s) | 1301 |
| SfaNI | GCATC | 3 cut(s) | 655, 775, 1103 |
| SfcI | CTRYAG | 2 cut(s) | 137, 1321 |
| SfoI | GGCGCC | 1 cut(s) | 514 |
| SinI | GGWCC | 1 cut(s) | 383 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 180, 345, 663, 999 |
| SmlI | CTYRAG | 1 cut(s) | 1234 |
| SmoI | CTYRAG | 1 cut(s) | 1234 |
| SpeI | ACTAGT | 1 cut(s) | 1367 |
| SphI | GCATGC | 1 cut(s) | 479 |
| Sse9I | AATT | 6 cut(s) | 49, 359, 424, 986, 1116, 1204 |
| SspDI | GGCGCC | 1 cut(s) | 512 |
| SspMI | CTAG | 5 cut(s) | 116, 836, 963, 1368, 1378 |
| StyD4I | CCNGG | 6 cut(s) | 212, 259, 415, 593, 688, 886 |
| StyI | CCWWGG | 1 cut(s) | 371 |
| TaaI | ACNGT | 7 cut(s) | 22, 295, 402, 466, 535, 826, 1217 |
| TaiI | ACGT | 2 cut(s) | 84, 755 |
| TaqI | TCGA | 1 cut(s) | 730 |
| TasI | AATT | 6 cut(s) | 49, 359, 424, 986, 1116, 1204 |
| TatI | WGTACW | 3 cut(s) | 57, 172, 1077 |
| TfiI | GAWTC | 1 cut(s) | 1223 |
| Tru1I | TTAA | 3 cut(s) | 362, 1100, 1119 |
| Tru9I | TTAA | 3 cut(s) | 362, 1100, 1119 |
| TscAI | CASTG | 3 cut(s) | 347, 407, 829 |
| TseFI | GTSAC | 1 cut(s) | 485 |
| TseI | GCWGC | 2 cut(s) | 1091, 1239 |
| Tsp45I | GTSAC | 1 cut(s) | 485 |
| TspDTI | ATGAA | 6 cut(s) | 104, 269, 299, 497, 582, 1217 |
| TspGWI | ACGGA | 2 cut(s) | 467, 855 |
| TspRI | CASTG | 3 cut(s) | 347, 407, 829 |
| Tth111I | GACNNNGTC | 1 cut(s) | 995 |
| VpaK11BI | GGWCC | 1 cut(s) | 383 |
| XapI | RAATTY | 3 cut(s) | 49, 986, 1204 |
| XceI | RCATGY | 2 cut(s) | 238, 479 |
| XmiI | GTMKAC | 1 cut(s) | 952 |
| XmnI | GAANNNNTTC | 1 cut(s) | 206 |
| XspI | CTAG | 5 cut(s) | 116, 836, 963, 1368, 1378 |
| ZrmI | AGTACT | 1 cut(s) | 59 |
| Zsp2I | ATGCAT | 2 cut(s) | 670, 790 |