Rroxscaffold_2G00094290

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000320
Physical Location & Seq
Forward (+)
15595122 .. 15598609
3488 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00094290.1

Sequence Viewer

Length: 1809 bp
ATGAAATCCCTGAAGAGAACTACAGCTGGTCCAGTGATGTCGACACAGTTTCCAGTGTTCGCTGTCCTTATTATCATCCTACAGCTCTTCATCTCTCTGGCTCGAGCCGAAGTGCATTACTACGATTTTATAGTTAAGGAGGTAAACTTCACAAGACTGTGTGAGTCAAAGCTCATGTTAGTAGTAAACGAGAGTTTTCCGGGTCCAGAATTACGAGCTCGCAAAGGAGATACAATTTATGTTAATGTTTACAATCAAGGATTTTATGGTTTCACCATTCACTGGCATGGAGTAAAGCAACCAAGAAATCCATGGTTTGATGGCCCGGAATATGTGACACAATGCCCAATTTCACCGGGAACAAATTTTACCTATCAAGTTCAATTGACCACAGAAGAAGGAACTGTATGGTGGCATGCTCATAGTGACTGGACGAGAGCTAGCGTTCACGGTACCATTGTCGTCTTGCCTGCTCTTGGAACAACATTTCCATTTCCTGACCCCGATGAAGAGGAGAGCATTGTGTTTGGATCTTGGTATTTGGGAAATTTGAAACAAACGATTGACGAGTGTATGGTGCCAGAAAATGCTACCAACTTACCTGAAGCAGATGCTTACACAATAAATGGCCAACCTGGGGATTTTGCTCTATGCTCCAAGAATTCAACATATCGTCTTAAGGTGGACTATGGCAAGACGTATCTTCTTCGGATACTGAACGCAAACATTGATGCAGAGATGTACTTTGCAATCGCTGAGCACAATCTCACTATGGTTGGCATGGACGGTTCCTATACCAAACCCATCAACACAAGCTACATAATGATAAGCCCTGGACAAACAATGGATGTGTTGCTCAACACAAATTCGACTCTGGGGAGCTATTACATGGTCGCAAGAGAATACGTTAGTGCTACAGTTAACTCAGTATCTAGCTATCTGGATGATGCTGTTGCAATCCTTGAATATAATGGCAACTATACCACTTCGGAAGCTCCGATATATCCTGAAAACCTTCCCATGTCCTTATCCCAAGAACCAGCATACGACTTCCTCAGGAAAATTAGAAGCTTAGCCACCCCAGAATATCCTATAAATGTCCCAAAAGAGAGTGACGTTACTAAGAGAATGTATATAACAGCTTCCTCCAACGCTCTTTACTGCACTCCAGATTTAGGTCCCACATGCGTCAACATATCTTGGGCTGCAAGCGTCAATAATATTAGTTGGGTTAATCCATCAGAATCTATCTTGCAAGCCTACTACAACAACATAAGCGAACCGGTTTATGAAACAGACTTCCCAGATGAGCCGCCTGTTTATTTTAACTTCACTGATGATTCATCAGCAATAGATGTAGGCTTAACAATCCAAGGGACAAAGGTGACGATGCTGGAATACAATGAAACAGTTGAGATGGTGTTTCAAGGAACTGATGTGGAGGGAGCCGCCGTGAATCATCCAATGCACGTGCATGGACTTAGCTTTTATGTGCTTGGTTTTGGTTTTGGGAATTACTTCGCCGAGAGGGATTCAAAAACCTTCAATTTGATTGATCCTCCTTATGTAGCAACGTTCATAACTCCAAAAAATGGATGGGTAGCTCTCAGATTCGTAGCGAATAATCCAGGTGTATGGTTTTGGCATTGTCATATGGAGAGACACTTGACGTGGGGTATGGAGTCTGCTTTTATAGTGAAGAACGGGGGCACACCTGAGACTACTATGCTCCCTCCTCCGGCTACAATGCCTTCCTGTGAAGTTCCATTGAAGTCTTCGGCCCCTAGAGAAGCAACTAGAACGGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00053 ko01100 map00053 map01100
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

602

Amino Acids

67.52

Weight (kDa)

4.84

Isoelectric Point (pI)

41.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_3 PF07732 45 - 157 1.1e-37 Multicopper oxidase
Cu-oxidase PF00394 173 - 326 9.3e-33 Multicopper oxidase
Cu-oxidase_2 PF07731 434 - 568 4.6e-35 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000320)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g12640 FvH4_2g18980 FvH4_2g18990 FvH4_2g40270 FvH4_2g40270 FvH4_4g22740 FvH4_4g22740 FvH4_5g35760 FvH4_5g35760 FvH4_5g35760 FvH4_5g35760 FvH4_5g35760 FvH4_5g35760 FvH4_5g35760 FvH4_5g35760 FvH4_5g36460 FvH4_5g36460 FvH4_6g39950 FvH4_6g39970 FvH4_6g39983 FvH4_6g39990
rosa_chinensis RchiOBHm_Chr2g0154481 RchiOBHm_Chr2g0154511 RchiOBHm_Chr4g0428961 RchiOBHm_Chr6g0252431 RchiOBHm_Chr6g0273301 RchiOBHm_Chr6g0273631 RchiOBHm_Chr6g0273651 RchiOBHm_Chr6g0284751 RchiOBHm_Chr6g0284761 RchiOBHm_Chr6g0300491 RchiOBHm_Chr7g0237421 RchiOBHm_Chr7g0238581
rosa_laevigata RLG00000000906 RLG00000001006 RLG00000007110 RLG00000011333 RLG00000012721 RLG00000013605 RLG00000013626 RLG00000020780 RLG00000020782 RLG00000020783 RLG00000020786
rosa_multiflora Rmu_co8321753.1_g000001 Rmu_sc0000239.1_g000020 Rmu_sc0000686.1_g000001 Rmu_sc0000686.1_g000003 Rmu_sc0000686.1_g000005 Rmu_sc0001476.1_g000011 Rmu_sc0002231.1_g000002 Rmu_sc0002231.1_g000016 Rmu_sc0002690.1_g000003 Rmu_sc0002717.1_g000015 Rmu_sc0002923.1_g000026 Rmu_sc0003808.1_g000016 Rmu_sc0006475.1_g000008 Rmu_sc0006475.1_g000012 Rmu_sc0006475.1_g000018 Rmu_sc0014815.1_g000004 Rmu_sc0015313.1_g000012
rosa_roxburghii Rroxscaffold_2G00094270 Rroxscaffold_2G00094290 Rroxscaffold_3G00222950 Rroxscaffold_3G00224250 Rroxscaffold_5G00370630 Rroxscaffold_7G00167740 Rroxscaffold_7G00184410 Rroxscaffold_7G00195190 Rroxscaffold_7G00195460
rosa_rugosa Rorug02G0444600 Rorug04G0231100 Rorug04G0231200 Rorug06G0074000 Rorug06G0076800 Rorug06G0166400 Rorug06G0166500 Rorug06G0166600 Rorug06G0296400 Rorug07G0303000
rosa_samantha Rh2CG494600 Rh2CG494700 Rh2DG530800 Rh2DG530900 Rh2DG531000 Rh4AG287500 Rh4AG403800 Rh4BG293400 Rh4BG415100 Rh4DG290500 Rh6AG191000 Rh6AG191300 Rh6BG192500 Rh6BG194800 Rh6BG195200 Rh6BG279500 Rh6BG279600 Rh6DG183900 Rh6DG186600 Rh6DG274300 Rh6DG274500 Rh6DG409500 Rh7AG460600 Rh7AG468200 Rh7BG430200
rosa_wichuraiana Rw2G041820 Rw2G041830 Rw2G041840 Rw4G024930 Rw6G016470 Rw6G016720 Rw6G016730 Rw6G024020 Rw6G024030 Rw6G035790 Rw7G038130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 452
AccB1I GGYRCC 2 cut(s) 452, 577
AccB7I CCANNNNNTGG 2 cut(s) 282, 1592
AccI GTMKAC 1 cut(s) 41
AciI CCGC 2 cut(s) 1313, 1449
AclI AACGTT 1 cut(s) 1574
AclWI GGATC 2 cut(s) 538, 1550
AcoI YGGCCR 1 cut(s) 628
AcsI RAATTY 4 cut(s) 364, 547, 661, 865
AcuI CTGAAG 2 cut(s) 32, 624
AcvI CACGTG 1 cut(s) 1471
AfaI GTAC 2 cut(s) 454, 743
AfiI CCNNNNNNNGG 6 cut(s) 282, 476, 637, 1175, 1592, 1738
AflII CTTAAG 1 cut(s) 677
AgeI ACCGGT 1 cut(s) 1282
AgsI TTSAA 8 cut(s) 383, 553, 666, 965, 1427, 1536, 1546, 1771
AjiI CACGTC 1 cut(s) 1671
AjnI CCWGG 3 cut(s) 634, 832, 1627
AloI GAACNNNNNNTCC 2 cut(s) 472, 504
Alw21I GWGCWC 2 cut(s) 220, 762
Alw26I GTCTC 2 cut(s) 1654, 1712
AlwI GGATC 2 cut(s) 538, 1550
Ama87I CYCGRG 1 cut(s) 102
AoxI GGCC 3 cut(s) 322, 628, 1779
ApeKI GCWGC 1 cut(s) 1205
ApoI RAATTY 4 cut(s) 364, 547, 661, 865
AsiGI ACCGGT 1 cut(s) 1282
Asp700I GAANNNNTTC 2 cut(s) 1014, 1517
Asp718I GGTACC 1 cut(s) 452
AspS9I GGNCC 5 cut(s) 29, 203, 323, 1178, 1780
AsuC2I CCSGG 3 cut(s) 201, 326, 357
AsuHPI GGTGA 3 cut(s) 265, 345, 1396
AsuNHI GCTAGC 1 cut(s) 440
AvaI CYCGRG 1 cut(s) 102
AvaII GGWCC 3 cut(s) 29, 203, 1178
AxyI CCTNAGG 1 cut(s) 1055
BaeGI GKGCMC 1 cut(s) 1712
BaeI ACNNNNGTAYC 2 cut(s) 444, 477
BalI TGGCCA 1 cut(s) 630
BanI GGYRCC 2 cut(s) 452, 577
BanII GRGCYC 1 cut(s) 220
BbrPI CACGTG 1 cut(s) 1471
BbsI GAAGAC 1 cut(s) 1767
Bbv12I GWGCWC 2 cut(s) 220, 762
BbvI GCAGC 1 cut(s) 1192
BccI CCATC 5 cut(s) 314, 812, 1246, 1411, 1590
BceAI ACGGC 1 cut(s) 1436
BciT130I CCWGG 3 cut(s) 636, 834, 1629
BciVI GTATCC 1 cut(s) 705
BcnI CCSGG 3 cut(s) 201, 326, 357
BcoDI GTCTC 2 cut(s) 1654, 1712
BfaI CTAG 4 cut(s) 441, 933, 1785, 1797
BfmI CTRYAG 3 cut(s) 21, 80, 915
BfrI CTTAAG 1 cut(s) 677
BfuI GTATCC 1 cut(s) 705
BisI GCNGC 3 cut(s) 1206, 1313, 1449
BlpI GCTNAGC 2 cut(s) 756, 1072
BlsI GCNGC 3 cut(s) 1207, 1314, 1450
Bme1390I CCNGG 6 cut(s) 201, 326, 357, 636, 834, 1629
Bme18I GGWCC 3 cut(s) 29, 203, 1178
BmeT110I CYCGRG 1 cut(s) 102
BmgBI CACGTC 1 cut(s) 1671
BmgT120I GGNCC 5 cut(s) 29, 203, 323, 1178, 1780
BmiI GGNNCC 7 cut(s) 204, 454, 579, 790, 1180, 1447, 1782
BmrFI CCNGG 6 cut(s) 201, 326, 357, 636, 834, 1629
BmsI GCATC 4 cut(s) 601, 721, 937, 1380
BmtI GCTAGC 1 cut(s) 444
BpiI GAAGAC 1 cut(s) 1767
BpmI CTGGAG 1 cut(s) 1152
Bpu1102I GCTNAGC 2 cut(s) 756, 1072
BpuMI CCSGG 3 cut(s) 201, 326, 357
BsaAI YACGTR 1 cut(s) 1471
BsaJI CCNNGG 4 cut(s) 311, 635, 832, 1372
BsaWI WCCGGW 1 cut(s) 1282
BsaXI ACNNNNNCTCC 2 cut(s) 1437, 1467
Bsc4I CCNNNNNNNGG 6 cut(s) 282, 476, 637, 1175, 1592, 1738
Bse118I RCCGGY 1 cut(s) 1282
Bse1I ACTGG 4 cut(s) 32, 53, 287, 434
Bse21I CCTNAGG 1 cut(s) 1055
BseBI CCWGG 3 cut(s) 636, 834, 1629
BseDI CCNNGG 4 cut(s) 311, 635, 832, 1372
BseGI GGATG 5 cut(s) 75, 853, 949, 1459, 1601
BseLI CCNNNNNNNGG 6 cut(s) 282, 476, 637, 1175, 1592, 1738
BseMII CTCAG 5 cut(s) 747, 939, 1069, 1621, 1707
BseNI ACTGG 4 cut(s) 32, 53, 287, 434
BseRI GAGGAG 2 cut(s) 527, 1725
BseSI GKGCMC 1 cut(s) 1712
BseXI GCAGC 1 cut(s) 1192
BsgI GTGCAG 1 cut(s) 1147
BshFI GGCC 3 cut(s) 324, 630, 1781
BshNI GGYRCC 2 cut(s) 452, 577
BshTI ACCGGT 1 cut(s) 1282
BsiHKAI GWGCWC 2 cut(s) 220, 762
BsiHKCI CYCGRG 1 cut(s) 102
BsiSI CCGG 5 cut(s) 200, 326, 356, 1283, 1739
BslFI GGGAC 3 cut(s) 1085, 1164, 1390
BslI CCNNNNNNNGG 6 cut(s) 282, 476, 637, 1175, 1592, 1738
BsmAI GTCTC 2 cut(s) 1654, 1712
BsmFI GGGAC 3 cut(s) 1085, 1164, 1390
BsnI GGCC 3 cut(s) 324, 630, 1781
BsoBI CYCGRG 1 cut(s) 102
Bsp1286I GDGCHC 3 cut(s) 220, 762, 1712
Bsp143I GATC 2 cut(s) 530, 1555
Bsp1720I GCTNAGC 2 cut(s) 756, 1072
Bsp19I CCATGG 1 cut(s) 311
BspACI CCGC 2 cut(s) 1313, 1449
BspANI GGCC 3 cut(s) 324, 630, 1781
BspCNI CTCAG 5 cut(s) 748, 938, 1068, 1620, 1708
BspLI GGNNCC 7 cut(s) 204, 454, 579, 790, 1180, 1447, 1782
BspOI GCTAGC 1 cut(s) 444
BspPI GGATC 2 cut(s) 538, 1550
BspQI GCTCTTC 1 cut(s) 92
BspT107I GGYRCC 2 cut(s) 452, 577
BspTI CTTAAG 1 cut(s) 677
BsrFI RCCGGY 1 cut(s) 1282
BsrI ACTGG 4 cut(s) 32, 53, 287, 434
BssAI RCCGGY 1 cut(s) 1282
BssECI CCNNGG 4 cut(s) 311, 635, 832, 1372
BssMI GATC 2 cut(s) 530, 1555
BssT1I CCWWGG 2 cut(s) 311, 1372
Bst2UI CCWGG 3 cut(s) 636, 834, 1629
Bst4CI ACNGT 7 cut(s) 48, 159, 406, 452, 788, 919, 1411
Bst6I CTCTTC 3 cut(s) 8, 92, 504
BstAFI CTTAAG 1 cut(s) 677
BstBAI YACGTR 1 cut(s) 1471
BstC8I GCNNGC 6 cut(s) 220, 417, 442, 471, 1210, 1257
BstDEI CTNAG 8 cut(s) 756, 925, 1055, 1072, 1122, 1481, 1607, 1716
BstDSI CCRYGG 1 cut(s) 311
BstF5I GGATG 5 cut(s) 75, 853, 949, 1459, 1601
BstKTI GATC 2 cut(s) 533, 1558
BstMAI GTCTC 2 cut(s) 1654, 1712
BstMBI GATC 2 cut(s) 530, 1555
BstNI CCWGG 3 cut(s) 636, 834, 1629
BstNSI RCATGY 2 cut(s) 419, 1188
BstSCI CCNGG 6 cut(s) 199, 324, 355, 634, 832, 1627
BstSFI CTRYAG 3 cut(s) 21, 80, 915
BstSLI GKGCMC 1 cut(s) 1712
BstV1I GCAGC 1 cut(s) 1192
BstV2I GAAGAC 1 cut(s) 1767
BstX2I RGATCY 1 cut(s) 530
BstXI CCANNNNNNTGG 1 cut(s) 1635
BstYI RGATCY 1 cut(s) 530
Bsu36I CCTNAGG 1 cut(s) 1055
BsuI GTATCC 1 cut(s) 705
BsuRI GGCC 3 cut(s) 324, 630, 1781
BtgI CCRYGG 1 cut(s) 311
BtrI CACGTC 1 cut(s) 1671
BtsCI GGATG 5 cut(s) 75, 853, 949, 1459, 1601
BtsIMutI CAGTG 4 cut(s) 39, 60, 280, 1332
Cac8I GCNNGC 6 cut(s) 220, 417, 442, 471, 1210, 1257
Cfr10I RCCGGY 1 cut(s) 1282
Cfr13I GGNCC 5 cut(s) 29, 203, 323, 1178, 1780
CseI GACGC 2 cut(s) 1177, 1201
Csp6I GTAC 2 cut(s) 453, 742
CspAI ACCGGT 1 cut(s) 1282
CviAII CATG 9 cut(s) 175, 287, 312, 416, 781, 889, 1021, 1185, 1475
CviQI GTAC 2 cut(s) 453, 742
DdeI CTNAG 8 cut(s) 756, 925, 1055, 1072, 1122, 1481, 1607, 1716
DpnI GATC 2 cut(s) 532, 1557
DpnII GATC 2 cut(s) 530, 1555
EaeI YGGCCR 1 cut(s) 628
Eam1104I CTCTTC 3 cut(s) 8, 92, 504
EarI CTCTTC 3 cut(s) 8, 92, 504
Ecl136II GAGCTC 1 cut(s) 218
Eco130I CCWWGG 2 cut(s) 311, 1372
Eco24I GRGCYC 1 cut(s) 220
Eco47I GGWCC 3 cut(s) 29, 203, 1178
Eco53kI GAGCTC 1 cut(s) 218
Eco57I CTGAAG 2 cut(s) 32, 624
Eco72I CACGTG 1 cut(s) 1471
Eco81I CCTNAGG 1 cut(s) 1055
Eco88I CYCGRG 1 cut(s) 102
EcoICRI GAGCTC 1 cut(s) 218
EcoO109I RGGNCCY 1 cut(s) 1178
EcoRI GAATTC 1 cut(s) 661
EcoRII CCWGG 3 cut(s) 634, 832, 1627
EcoT14I CCWWGG 2 cut(s) 311, 1372
EcoT38I GRGCYC 1 cut(s) 220
ErhI CCWWGG 2 cut(s) 311, 1372
FaeI CATG 9 cut(s) 178, 290, 315, 419, 784, 892, 1024, 1188, 1478
FaqI GGGAC 3 cut(s) 1085, 1164, 1390
FatI CATG 9 cut(s) 174, 286, 311, 415, 780, 888, 1020, 1184, 1474
FauNDI CATATG 1 cut(s) 1653
FblI GTMKAC 1 cut(s) 41
Fnu4HI GCNGC 3 cut(s) 1206, 1313, 1449
FokI GGATG 5 cut(s) 62, 860, 956, 1446, 1608
FriOI GRGCYC 1 cut(s) 220
Fsp4HI GCNGC 3 cut(s) 1206, 1313, 1449
FspBI CTAG 4 cut(s) 441, 933, 1785, 1797
GluI GCNGC 3 cut(s) 1206, 1313, 1449
GsuI CTGGAG 1 cut(s) 1152
HaeIII GGCC 3 cut(s) 324, 630, 1781
HapII CCGG 5 cut(s) 200, 326, 356, 1283, 1739
HgaI GACGC 2 cut(s) 1177, 1201
Hin1II CATG 9 cut(s) 178, 290, 315, 419, 784, 892, 1024, 1188, 1478
HincII GTYRAC 3 cut(s) 42, 922, 1192
HindII GTYRAC 3 cut(s) 42, 922, 1192
HindIII AAGCTT 1 cut(s) 1069
HinfI GANTC 8 cut(s) 164, 871, 1244, 1340, 1456, 1532, 1611, 1682
HpaI GTTAAC 1 cut(s) 922
HpaII CCGG 5 cut(s) 200, 326, 356, 1283, 1739
HphI GGTGA 3 cut(s) 265, 345, 1396
Hpy166II GTNNAC 8 cut(s) 42, 145, 187, 250, 448, 685, 922, 1192
Hpy188I TCNGA 5 cut(s) 711, 991, 999, 1243, 1610
Hpy188III TCNNGA 6 cut(s) 206, 497, 941, 1007, 1057, 1169
Hpy8I GTNNAC 8 cut(s) 42, 145, 187, 250, 448, 685, 922, 1192
HpyAV CCTTC 4 cut(s) 392, 1025, 1552, 1761
HpyCH4III ACNGT 7 cut(s) 48, 159, 406, 452, 788, 919, 1411
HpyCH4IV ACGT 6 cut(s) 698, 906, 1116, 1470, 1574, 1670
HpyCH4V TGCA 9 cut(s) 115, 734, 749, 956, 1164, 1208, 1255, 1468, 1474
HpyF3I CTNAG 8 cut(s) 756, 925, 1055, 1072, 1122, 1481, 1607, 1716
HpySE526I ACGT 6 cut(s) 698, 906, 1116, 1470, 1574, 1670
Hsp92II CATG 9 cut(s) 178, 290, 315, 419, 784, 892, 1024, 1188, 1478
KpnI GGTACC 1 cut(s) 456
KspAI GTTAAC 1 cut(s) 922
Kzo9I GATC 2 cut(s) 530, 1555
LguI GCTCTTC 1 cut(s) 92
LmnI GCTCC 5 cut(s) 659, 879, 1000, 1445, 1734
Lsp1109I GCAGC 1 cut(s) 1192
LweI GCATC 4 cut(s) 601, 721, 937, 1380
MaeI CTAG 4 cut(s) 441, 933, 1785, 1797
MaeII ACGT 6 cut(s) 698, 906, 1116, 1470, 1574, 1670
MaeIII GTNAC 5 cut(s) 334, 425, 1112, 1117, 1384
MalI GATC 2 cut(s) 532, 1557
MboI GATC 2 cut(s) 530, 1555
MboII GAAGA 8 cut(s) 25, 79, 407, 521, 695, 698, 1711, 1767
MfeI CAATTG 1 cut(s) 383
MflI RGATCY 1 cut(s) 530
MhlI GDGCHC 3 cut(s) 220, 762, 1712
MlsI TGGCCA 1 cut(s) 630
MluNI TGGCCA 1 cut(s) 630
MlyI GAGTC 3 cut(s) 173, 865, 1691
MmeI TCCRAC 1 cut(s) 1173
MnlI CCTC 9 cut(s) 133, 505, 1064, 1156, 1435, 1521, 1569, 1743, 1746
Mox20I TGGCCA 1 cut(s) 630
MroXI GAANNNNTTC 2 cut(s) 1014, 1517
MscI TGGCCA 1 cut(s) 630
MseI TTAA 7 cut(s) 135, 243, 678, 921, 1233, 1326, 1364
MslI CAYNNNNRTG 2 cut(s) 285, 1473
Msp20I TGGCCA 1 cut(s) 630
MspA1I CMGCKG 1 cut(s) 26
MspCI CTTAAG 1 cut(s) 677
MspI CCGG 5 cut(s) 200, 326, 356, 1283, 1739
MspR9I CCNGG 6 cut(s) 201, 326, 357, 636, 834, 1629
MunI CAATTG 1 cut(s) 383
MvaI CCWGG 3 cut(s) 636, 834, 1629
NciI CCSGG 3 cut(s) 201, 326, 357
NcoI CCATGG 1 cut(s) 311
NdeI CATATG 1 cut(s) 1653
NdeII GATC 2 cut(s) 530, 1555
NheI GCTAGC 1 cut(s) 440
NlaIII CATG 9 cut(s) 178, 290, 315, 419, 784, 892, 1024, 1188, 1478
NlaIV GGNNCC 7 cut(s) 204, 454, 579, 790, 1180, 1447, 1782
NmeAIII GCCGAG 1 cut(s) 1549
NmuCI GTSAC 4 cut(s) 334, 425, 1112, 1384
NspI RCATGY 2 cut(s) 419, 1188
PaeI GCATGC 1 cut(s) 419
PaeR7I CTCGAG 1 cut(s) 102
PciSI GCTCTTC 1 cut(s) 92
PcsI WCGNNNNNNNCGW 1 cut(s) 995
PdmI GAANNNNTTC 2 cut(s) 1014, 1517
PfeI GAWTC 5 cut(s) 1244, 1340, 1456, 1532, 1611
PflMI CCANNNNNTGG 2 cut(s) 282, 1592
PinAI ACCGGT 1 cut(s) 1282
PkrI GCNGC 3 cut(s) 1207, 1314, 1450
PleI GAGTC 3 cut(s) 172, 865, 1690
PmaCI CACGTG 1 cut(s) 1471
PmlI CACGTG 1 cut(s) 1471
PpsI GAGTC 3 cut(s) 172, 865, 1690
Ppu21I YACGTR 1 cut(s) 1471
PpuMI RGGWCCY 1 cut(s) 1178
Psp124BI GAGCTC 1 cut(s) 220
Psp1406I AACGTT 1 cut(s) 1574
Psp5II RGGWCCY 1 cut(s) 1178
Psp6I CCWGG 3 cut(s) 634, 832, 1627
PspCI CACGTG 1 cut(s) 1471
PspGI CCWGG 3 cut(s) 634, 832, 1627
PspN4I GGNNCC 7 cut(s) 204, 454, 579, 790, 1180, 1447, 1782
PspPI GGNCC 5 cut(s) 29, 203, 323, 1178, 1780
PspPPI RGGWCCY 1 cut(s) 1178
PspXI VCTCGAGB 1 cut(s) 102
PsrI GAACNNNNNNTAC 2 cut(s) 352, 384
PsuI RGATCY 1 cut(s) 530
PvuII CAGCTG 1 cut(s) 26
RsaI GTAC 2 cut(s) 454, 743
RsaNI GTAC 2 cut(s) 453, 742
RseI CAYNNNNRTG 2 cut(s) 285, 1473
SacI GAGCTC 1 cut(s) 220
SalI GTCGAC 1 cut(s) 40
SapI GCTCTTC 1 cut(s) 92
SaqAI TTAA 7 cut(s) 135, 243, 678, 921, 1233, 1326, 1364
SatI GCNGC 3 cut(s) 1206, 1313, 1449
Sau3AI GATC 2 cut(s) 530, 1555
Sau96I GGNCC 5 cut(s) 29, 203, 323, 1178, 1780
SchI GAGTC 3 cut(s) 173, 865, 1691
ScrFI CCNGG 6 cut(s) 201, 326, 357, 636, 834, 1629
SduI GDGCHC 3 cut(s) 220, 762, 1712
SfaNI GCATC 4 cut(s) 601, 721, 937, 1380
SfcI CTRYAG 3 cut(s) 21, 80, 915
Sfr274I CTCGAG 1 cut(s) 102
SinI GGWCC 3 cut(s) 29, 203, 1178
SlaI CTCGAG 1 cut(s) 102
SmiMI CAYNNNNRTG 2 cut(s) 285, 1473
SmlI CTYRAG 2 cut(s) 102, 677
SmoI CTYRAG 2 cut(s) 102, 677
SphI GCATGC 1 cut(s) 419
SsiI CCGC 2 cut(s) 1313, 1449
SspI AATATT 1 cut(s) 1222
SspMI CTAG 4 cut(s) 441, 933, 1785, 1797
SstI GAGCTC 1 cut(s) 220
StyD4I CCNGG 6 cut(s) 199, 324, 355, 634, 832, 1627
StyI CCWWGG 2 cut(s) 311, 1372
TaaI ACNGT 7 cut(s) 48, 159, 406, 452, 788, 919, 1411
TaiI ACGT 6 cut(s) 701, 909, 1119, 1473, 1577, 1673
TaqI TCGA 3 cut(s) 41, 103, 869
TatI WGTACW 1 cut(s) 741
TauI GCSGC 2 cut(s) 1315, 1451
TfiI GAWTC 5 cut(s) 1244, 1340, 1456, 1532, 1611
Tru1I TTAA 7 cut(s) 135, 243, 678, 921, 1233, 1326, 1364
Tru9I TTAA 7 cut(s) 135, 243, 678, 921, 1233, 1326, 1364
TscAI CASTG 4 cut(s) 39, 60, 287, 1339
TseFI GTSAC 4 cut(s) 334, 425, 1112, 1384
TseI GCWGC 1 cut(s) 1205
Tsp45I GTSAC 4 cut(s) 334, 425, 1112, 1384
TspDTI ATGAA 7 cut(s) 17, 79, 522, 1305, 1332, 1419, 1567
TspRI CASTG 4 cut(s) 39, 60, 287, 1339
Van91I CCANNNNNTGG 2 cut(s) 282, 1592
Vha464I CTTAAG 1 cut(s) 677
VpaK11BI GGWCC 3 cut(s) 29, 203, 1178
XapI RAATTY 4 cut(s) 364, 547, 661, 865
XceI RCATGY 2 cut(s) 419, 1188
XcmI CCANNNNNNNNNTGG 2 cut(s) 309, 1593
XhoI CTCGAG 1 cut(s) 102
XmiI GTMKAC 1 cut(s) 41
XmnI GAANNNNTTC 2 cut(s) 1014, 1517
XspI CTAG 4 cut(s) 441, 933, 1785, 1797
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.