Lignin degradation and detoxification of lignin-derived products
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 1602 |
| Acc36I | ACCTGC | 2 cut(s) | 616, 1602 |
| AccB1I | GGYRCC | 1 cut(s) | 590 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 420, 1715 |
| AccBSI | CCGCTC | 1 cut(s) | 223 |
| AccI | GTMKAC | 1 cut(s) | 179 |
| AccII | CGCG | 1 cut(s) | 127 |
| AccIII | TCCGGA | 1 cut(s) | 1138 |
| AciI | CCGC | 2 cut(s) | 221, 1278 |
| AclWI | GGATC | 3 cut(s) | 676, 1472, 1673 |
| AcoI | YGGCCR | 1 cut(s) | 766 |
| AcsI | RAATTY | 3 cut(s) | 502, 685, 1003 |
| AcuI | CTGAAG | 2 cut(s) | 1104, 1578 |
| AfaI | GTAC | 3 cut(s) | 157, 1265, 1297 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 420, 614, 910, 1307, 1715, 1862 |
| AflII | CTTAAG | 1 cut(s) | 103 |
| AflIII | ACRYGT | 1 cut(s) | 987 |
| AgeI | ACCGGT | 1 cut(s) | 1401 |
| AgsI | TTSAA | 6 cut(s) | 64, 691, 1097, 1180, 1496, 1550 |
| AhlI | ACTAGT | 1 cut(s) | 161 |
| AjiI | CACGTC | 1 cut(s) | 988 |
| AjnI | CCWGG | 3 cut(s) | 337, 970, 1750 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 610, 642 |
| Alw21I | GWGCWC | 1 cut(s) | 358 |
| Alw26I | GTCTC | 2 cut(s) | 1777, 1835 |
| AlwI | GGATC | 3 cut(s) | 676, 1472, 1673 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 190 |
| Ama87I | CYCGRG | 1 cut(s) | 240 |
| Aor13HI | TCCGGA | 1 cut(s) | 1138 |
| AoxI | GGCC | 2 cut(s) | 460, 766 |
| ApeKI | GCWGC | 5 cut(s) | 129, 200, 719, 746, 1337 |
| ApoI | RAATTY | 3 cut(s) | 502, 685, 1003 |
| AseI | ATTAAT | 1 cut(s) | 762 |
| AsiGI | ACCGGT | 1 cut(s) | 1401 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 1146, 1640 |
| AspLEI | GCGC | 2 cut(s) | 127, 129 |
| AspS9I | GGNCC | 4 cut(s) | 19, 341, 461, 1310 |
| AsuC2I | CCSGG | 1 cut(s) | 495 |
| AsuHPI | GGTGA | 3 cut(s) | 403, 483, 1831 |
| AvaI | CYCGRG | 1 cut(s) | 240 |
| AvaII | GGWCC | 3 cut(s) | 19, 341, 1310 |
| BaeGI | GKGCMC | 1 cut(s) | 1835 |
| BanI | GGYRCC | 1 cut(s) | 590 |
| BanII | GRGCYC | 1 cut(s) | 358 |
| Bbv12I | GWGCWC | 1 cut(s) | 358 |
| BbvI | GCAGC | 5 cut(s) | 141, 187, 706, 758, 1324 |
| BccI | CCATC | 5 cut(s) | 452, 950, 1534, 1558, 1713 |
| BceAI | ACGGC | 1 cut(s) | 940 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 159, 193 |
| BciT130I | CCWGG | 3 cut(s) | 339, 972, 1752 |
| BciVI | GTATCC | 1 cut(s) | 361 |
| BcnI | CCSGG | 1 cut(s) | 495 |
| BcoDI | GTCTC | 2 cut(s) | 1777, 1835 |
| BcuI | ACTAGT | 1 cut(s) | 161 |
| BfaI | CTAG | 5 cut(s) | 162, 291, 573, 1475, 1517 |
| BfmI | CTRYAG | 4 cut(s) | 69, 97, 1053, 1186 |
| BfrI | CTTAAG | 1 cut(s) | 103 |
| BfuAI | ACCTGC | 2 cut(s) | 616, 1602 |
| BfuI | GTATCC | 1 cut(s) | 361 |
| BglII | AGATCT | 1 cut(s) | 50 |
| BisI | GCNGC | 5 cut(s) | 130, 201, 720, 747, 1338 |
| BlpI | GCTNAGC | 1 cut(s) | 1204 |
| BlsI | GCNGC | 5 cut(s) | 131, 202, 721, 748, 1339 |
| BmcAI | AGTACT | 1 cut(s) | 157 |
| Bme1390I | CCNGG | 4 cut(s) | 339, 495, 972, 1752 |
| Bme18I | GGWCC | 3 cut(s) | 19, 341, 1310 |
| BmeT110I | CYCGRG | 1 cut(s) | 240 |
| BmgBI | CACGTC | 1 cut(s) | 988 |
| BmgT120I | GGNCC | 4 cut(s) | 19, 341, 461, 1310 |
| BmiI | GGNNCC | 4 cut(s) | 592, 928, 1312, 1573 |
| BmrFI | CCNGG | 4 cut(s) | 339, 495, 972, 1752 |
| BmsI | GCATC | 3 cut(s) | 1069, 1076, 1503 |
| BoxI | GACNNNNGTC | 2 cut(s) | 708, 1505 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1640, 1672 |
| BpmI | CTGGAG | 1 cut(s) | 1284 |
| Bpu10I | CCTNAGC | 1 cut(s) | 636 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1204 |
| BpuMI | CCSGG | 1 cut(s) | 495 |
| Bsa29I | ATCGAT | 1 cut(s) | 867 |
| BsaAI | YACGTR | 1 cut(s) | 1690 |
| BsaJI | CCNNGG | 3 cut(s) | 449, 970, 1370 |
| BsaWI | WCCGGW | 2 cut(s) | 1138, 1401 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1560, 1590 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 420, 614, 910, 1307, 1715, 1862 |
| Bse118I | RCCGGY | 2 cut(s) | 768, 1401 |
| Bse1I | ACTGG | 3 cut(s) | 425, 572, 1481 |
| Bse3DI | GCAATG | 1 cut(s) | 888 |
| BseAI | TCCGGA | 1 cut(s) | 1138 |
| BseBI | CCWGG | 3 cut(s) | 339, 972, 1752 |
| BseCI | ATCGAT | 1 cut(s) | 867 |
| BseDI | CCNNGG | 3 cut(s) | 449, 970, 1370 |
| BseGI | GGATG | 3 cut(s) | 213, 1582, 1724 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 420, 614, 910, 1307, 1715, 1862 |
| BseMI | GCAATG | 1 cut(s) | 888 |
| BseMII | CTCAG | 3 cut(s) | 45, 627, 1830 |
| BseNI | ACTGG | 3 cut(s) | 425, 572, 1481 |
| BsePI | GCGCGC | 1 cut(s) | 125 |
| BseRI | GAGGAG | 1 cut(s) | 665 |
| BseSI | GKGCMC | 1 cut(s) | 1835 |
| BseXI | GCAGC | 5 cut(s) | 141, 187, 706, 758, 1324 |
| BseYI | CCCAGC | 1 cut(s) | 1359 |
| Bsh1236I | CGCG | 1 cut(s) | 127 |
| Bsh1285I | CGRYCG | 1 cut(s) | 1431 |
| BshFI | GGCC | 2 cut(s) | 462, 768 |
| BshNI | GGYRCC | 1 cut(s) | 590 |
| BshTI | ACCGGT | 1 cut(s) | 1401 |
| BshVI | ATCGAT | 1 cut(s) | 867 |
| BsiEI | CGRYCG | 1 cut(s) | 1431 |
| BsiHKAI | GWGCWC | 1 cut(s) | 358 |
| BsiHKCI | CYCGRG | 1 cut(s) | 240 |
| BsiSI | CCGG | 5 cut(s) | 494, 769, 1139, 1402, 1863 |
| BslFI | GGGAC | 4 cut(s) | 1217, 1296, 1513, 1667 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 420, 614, 910, 1307, 1715, 1862 |
| BsmAI | GTCTC | 2 cut(s) | 1777, 1835 |
| BsmFI | GGGAC | 4 cut(s) | 1217, 1296, 1513, 1667 |
| BsmI | GAATGC | 1 cut(s) | 1175 |
| BsnI | GGCC | 2 cut(s) | 462, 768 |
| BsoBI | CYCGRG | 1 cut(s) | 240 |
| Bsp1286I | GDGCHC | 2 cut(s) | 358, 1835 |
| Bsp13I | TCCGGA | 1 cut(s) | 1138 |
| Bsp1407I | TGTACA | 1 cut(s) | 1263 |
| Bsp143I | GATC | 6 cut(s) | 50, 654, 668, 1428, 1477, 1678 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1204 |
| Bsp19I | CCATGG | 1 cut(s) | 449 |
| BspACI | CCGC | 2 cut(s) | 221, 1278 |
| BspANI | GGCC | 2 cut(s) | 462, 768 |
| BspCNI | CTCAG | 3 cut(s) | 46, 628, 1831 |
| BspDI | ATCGAT | 1 cut(s) | 867 |
| BspEI | TCCGGA | 1 cut(s) | 1138 |
| BspFNI | CGCG | 1 cut(s) | 127 |
| BspLI | GGNNCC | 4 cut(s) | 592, 928, 1312, 1573 |
| BspMI | ACCTGC | 2 cut(s) | 616, 1602 |
| BspPI | GGATC | 3 cut(s) | 676, 1472, 1673 |
| BspQI | GCTCTTC | 1 cut(s) | 230 |
| BspT107I | GGYRCC | 1 cut(s) | 590 |
| BspTI | CTTAAG | 1 cut(s) | 103 |
| BsrBI | CCGCTC | 1 cut(s) | 223 |
| BsrDI | GCAATG | 1 cut(s) | 888 |
| BsrFI | RCCGGY | 2 cut(s) | 768, 1401 |
| BsrGI | TGTACA | 1 cut(s) | 1263 |
| BsrI | ACTGG | 3 cut(s) | 425, 572, 1481 |
| BssAI | RCCGGY | 2 cut(s) | 768, 1401 |
| BssECI | CCNNGG | 3 cut(s) | 449, 970, 1370 |
| BssHII | GCGCGC | 1 cut(s) | 125 |
| BssMI | GATC | 6 cut(s) | 50, 654, 668, 1428, 1477, 1678 |
| BssT1I | CCWWGG | 1 cut(s) | 449 |
| Bst2UI | CCWGG | 3 cut(s) | 339, 972, 1752 |
| Bst4CI | ACNGT | 7 cut(s) | 186, 590, 910, 1057, 1295, 1492, 1534 |
| Bst6I | CTCTTC | 4 cut(s) | 146, 230, 642, 1030 |
| BstAFI | CTTAAG | 1 cut(s) | 103 |
| BstAUI | TGTACA | 1 cut(s) | 1263 |
| BstBAI | YACGTR | 1 cut(s) | 1690 |
| BstC8I | GCNNGC | 7 cut(s) | 127, 358, 555, 580, 770, 1342, 1389 |
| BstDEI | CTNAG | 5 cut(s) | 54, 636, 1046, 1204, 1839 |
| BstDSI | CCRYGG | 1 cut(s) | 449 |
| BstF5I | GGATG | 3 cut(s) | 213, 1582, 1724 |
| BstFNI | CGCG | 1 cut(s) | 127 |
| BstHHI | GCGC | 2 cut(s) | 127, 129 |
| BstKTI | GATC | 6 cut(s) | 53, 657, 671, 1431, 1480, 1681 |
| BstMAI | GTCTC | 2 cut(s) | 1777, 1835 |
| BstMBI | GATC | 6 cut(s) | 50, 654, 668, 1428, 1477, 1678 |
| BstMCI | CGRYCG | 1 cut(s) | 1431 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 108, 353, 924 |
| BstNI | CCWGG | 3 cut(s) | 339, 972, 1752 |
| BstNSI | RCATGY | 2 cut(s) | 557, 1320 |
| BstPAI | GACNNNNGTC | 2 cut(s) | 708, 1505 |
| BstSCI | CCNGG | 4 cut(s) | 337, 493, 970, 1750 |
| BstSFI | CTRYAG | 4 cut(s) | 69, 97, 1053, 1186 |
| BstSLI | GKGCMC | 1 cut(s) | 1835 |
| BstSNI | TACGTA | 1 cut(s) | 1690 |
| BstUI | CGCG | 1 cut(s) | 127 |
| BstV1I | GCAGC | 5 cut(s) | 141, 187, 706, 758, 1324 |
| BstX2I | RGATCY | 3 cut(s) | 50, 668, 1477 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 16, 1758 |
| BstYI | RGATCY | 3 cut(s) | 50, 668, 1477 |
| Bsu15I | ATCGAT | 1 cut(s) | 867 |
| BsuI | GTATCC | 1 cut(s) | 361 |
| BsuRI | GGCC | 2 cut(s) | 462, 768 |
| BsuTUI | ATCGAT | 1 cut(s) | 867 |
| BtgI | CCRYGG | 1 cut(s) | 449 |
| BtgZI | GCGATG | 1 cut(s) | 1481 |
| BtrI | CACGTC | 1 cut(s) | 988 |
| BtsCI | GGATG | 3 cut(s) | 213, 1582, 1724 |
| BtsIMutI | CAGTG | 6 cut(s) | 137, 170, 418, 906, 1455, 1488 |
| BveI | ACCTGC | 2 cut(s) | 616, 1602 |
| Cac8I | GCNNGC | 7 cut(s) | 127, 358, 555, 580, 770, 1342, 1389 |
| CaiI | CAGNNNCTG | 1 cut(s) | 190 |
| CfoI | GCGC | 2 cut(s) | 127, 129 |
| Cfr10I | RCCGGY | 2 cut(s) | 768, 1401 |
| Cfr13I | GGNCC | 4 cut(s) | 19, 341, 461, 1310 |
| ClaI | ATCGAT | 1 cut(s) | 867 |
| CseI | GACGC | 3 cut(s) | 725, 1309, 1333 |
| Csp6I | GTAC | 3 cut(s) | 156, 1264, 1296 |
| CspAI | ACCGGT | 1 cut(s) | 1401 |
| CviQI | GTAC | 3 cut(s) | 156, 1264, 1296 |
| DdeI | CTNAG | 5 cut(s) | 54, 636, 1046, 1204, 1839 |
| DpnI | GATC | 6 cut(s) | 52, 656, 670, 1430, 1479, 1680 |
| DpnII | GATC | 6 cut(s) | 50, 654, 668, 1428, 1477, 1678 |
| EaeI | YGGCCR | 1 cut(s) | 766 |
| Eam1104I | CTCTTC | 4 cut(s) | 146, 230, 642, 1030 |
| EarI | CTCTTC | 4 cut(s) | 146, 230, 642, 1030 |
| Ecl136II | GAGCTC | 1 cut(s) | 356 |
| Eco105I | TACGTA | 1 cut(s) | 1690 |
| Eco130I | CCWWGG | 1 cut(s) | 449 |
| Eco24I | GRGCYC | 1 cut(s) | 358 |
| Eco47I | GGWCC | 3 cut(s) | 19, 341, 1310 |
| Eco53kI | GAGCTC | 1 cut(s) | 356 |
| Eco57I | CTGAAG | 2 cut(s) | 1104, 1578 |
| Eco88I | CYCGRG | 1 cut(s) | 240 |
| EcoICRI | GAGCTC | 1 cut(s) | 356 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1310 |
| EcoRII | CCWGG | 3 cut(s) | 337, 970, 1750 |
| EcoT14I | CCWWGG | 1 cut(s) | 449 |
| EcoT38I | GRGCYC | 1 cut(s) | 358 |
| ErhI | CCWWGG | 1 cut(s) | 449 |
| FaqI | GGGAC | 4 cut(s) | 1217, 1296, 1513, 1667 |
| FblI | GTMKAC | 1 cut(s) | 179 |
| Fnu4HI | GCNGC | 5 cut(s) | 130, 201, 720, 747, 1338 |
| FokI | GGATG | 3 cut(s) | 200, 1569, 1731 |
| FriOI | GRGCYC | 1 cut(s) | 358 |
| Fsp4HI | GCNGC | 5 cut(s) | 130, 201, 720, 747, 1338 |
| FspBI | CTAG | 5 cut(s) | 162, 291, 573, 1475, 1517 |
| GlaI | GCGC | 2 cut(s) | 126, 128 |
| GluI | GCNGC | 5 cut(s) | 130, 201, 720, 747, 1338 |
| GsaI | CCCAGC | 1 cut(s) | 1363 |
| GsuI | CTGGAG | 1 cut(s) | 1284 |
| HaeIII | GGCC | 2 cut(s) | 462, 768 |
| HapII | CCGG | 5 cut(s) | 494, 769, 1139, 1402, 1863 |
| HgaI | GACGC | 3 cut(s) | 725, 1309, 1333 |
| HhaI | GCGC | 2 cut(s) | 127, 129 |
| Hin6I | GCGC | 2 cut(s) | 125, 127 |
| HinP1I | GCGC | 2 cut(s) | 125, 127 |
| HincII | GTYRAC | 5 cut(s) | 180, 283, 525, 703, 1488 |
| HindII | GTYRAC | 5 cut(s) | 180, 283, 525, 703, 1488 |
| HindIII | AAGCTT | 1 cut(s) | 1201 |
| HinfI | GANTC | 8 cut(s) | 302, 1147, 1154, 1376, 1579, 1655, 1734, 1805 |
| HpaI | GTTAAC | 1 cut(s) | 1488 |
| HpaII | CCGG | 5 cut(s) | 494, 769, 1139, 1402, 1863 |
| HphI | GGTGA | 3 cut(s) | 403, 483, 1831 |
| Hpy188I | TCNGA | 9 cut(s) | 55, 668, 849, 1034, 1072, 1123, 1199, 1371, 1733 |
| Hpy188III | TCNNGA | 6 cut(s) | 635, 713, 1139, 1151, 1301, 1510 |
| Hpy99I | CGWCG | 1 cut(s) | 816 |
| HpyAV | CCTTC | 1 cut(s) | 530 |
| HpyCH4III | ACNGT | 7 cut(s) | 186, 590, 910, 1057, 1295, 1492, 1534 |
| HpyCH4IV | ACGT | 3 cut(s) | 806, 987, 1689 |
| HpyCH4V | TGCA | 8 cut(s) | 89, 722, 1067, 1088, 1340, 1387, 1591, 1597 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 108, 353, 924 |
| HpyF3I | CTNAG | 5 cut(s) | 54, 636, 1046, 1204, 1839 |
| HpySE526I | ACGT | 3 cut(s) | 806, 987, 1689 |
| HspAI | GCGC | 2 cut(s) | 125, 127 |
| Kpn2I | TCCGGA | 1 cut(s) | 1138 |
| KroI | GCCGGC | 1 cut(s) | 768 |
| KroNI | GCCGGC | 1 cut(s) | 770 |
| KspAI | GTTAAC | 1 cut(s) | 1488 |
| Kzo9I | GATC | 6 cut(s) | 50, 654, 668, 1428, 1477, 1678 |
| LguI | GCTCTTC | 1 cut(s) | 230 |
| LmnI | GCTCC | 5 cut(s) | 797, 932, 1017, 1132, 1857 |
| Lsp1109I | GCAGC | 5 cut(s) | 141, 187, 706, 758, 1324 |
| LweI | GCATC | 3 cut(s) | 1069, 1076, 1503 |
| MaeI | CTAG | 5 cut(s) | 162, 291, 573, 1475, 1517 |
| MaeII | ACGT | 3 cut(s) | 806, 987, 1689 |
| MaeIII | GTNAC | 6 cut(s) | 472, 563, 752, 1690, 1702, 1772 |
| MalI | GATC | 6 cut(s) | 52, 656, 670, 1430, 1479, 1680 |
| MbiI | CCGCTC | 1 cut(s) | 223 |
| MboI | GATC | 6 cut(s) | 50, 654, 668, 1428, 1477, 1678 |
| MboII | GAAGA | 8 cut(s) | 163, 217, 545, 659, 833, 836, 1047, 1834 |
| MflI | RGATCY | 3 cut(s) | 50, 668, 1477 |
| MhlI | GDGCHC | 2 cut(s) | 358, 1835 |
| MluCI | AATT | 9 cut(s) | 59, 372, 502, 685, 759, 1003, 1636, 1669, 1674 |
| MlyI | GAGTC | 2 cut(s) | 1649, 1814 |
| MmeI | TCCRAC | 1 cut(s) | 1012 |
| MnlI | CCTC | 9 cut(s) | 49, 271, 643, 914, 1365, 1562, 1644, 1692, 1870 |
| MroI | TCCGGA | 1 cut(s) | 1138 |
| MroNI | GCCGGC | 1 cut(s) | 768 |
| MroXI | GAANNNNTTC | 2 cut(s) | 1146, 1640 |
| MseI | TTAA | 8 cut(s) | 104, 273, 381, 762, 1449, 1487, 1668, 1673 |
| MslI | CAYNNNNRTG | 2 cut(s) | 423, 1596 |
| MspA1I | CMGCKG | 1 cut(s) | 749 |
| MspCI | CTTAAG | 1 cut(s) | 103 |
| MspI | CCGG | 5 cut(s) | 494, 769, 1139, 1402, 1863 |
| MspR9I | CCNGG | 4 cut(s) | 339, 495, 972, 1752 |
| Mva1269I | GAATGC | 1 cut(s) | 1175 |
| MvaI | CCWGG | 3 cut(s) | 339, 972, 1752 |
| MvnI | CGCG | 1 cut(s) | 127 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 108, 353, 924 |
| NaeI | GCCGGC | 1 cut(s) | 770 |
| NciI | CCSGG | 1 cut(s) | 495 |
| NcoI | CCATGG | 1 cut(s) | 449 |
| NdeII | GATC | 6 cut(s) | 50, 654, 668, 1428, 1477, 1678 |
| NgoMIV | GCCGGC | 1 cut(s) | 768 |
| NlaIV | GGNNCC | 4 cut(s) | 592, 928, 1312, 1573 |
| NmeAIII | GCCGAG | 1 cut(s) | 1672 |
| NmuCI | GTSAC | 3 cut(s) | 472, 563, 1772 |
| NspI | RCATGY | 2 cut(s) | 557, 1320 |
| PaeI | GCATGC | 1 cut(s) | 557 |
| PaeR7I | CTCGAG | 1 cut(s) | 240 |
| PaqCI | CACCTGC | 1 cut(s) | 1602 |
| PauI | GCGCGC | 1 cut(s) | 125 |
| PciSI | GCTCTTC | 1 cut(s) | 230 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 864 |
| PctI | GAATGC | 1 cut(s) | 1175 |
| PdiI | GCCGGC | 1 cut(s) | 770 |
| PdmI | GAANNNNTTC | 2 cut(s) | 1146, 1640 |
| PfeI | GAWTC | 6 cut(s) | 302, 1147, 1154, 1376, 1579, 1734 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 420, 1715 |
| PinAI | ACCGGT | 1 cut(s) | 1401 |
| PkrI | GCNGC | 5 cut(s) | 131, 202, 721, 748, 1339 |
| Ple19I | CGATCG | 1 cut(s) | 1431 |
| PleI | GAGTC | 2 cut(s) | 1649, 1813 |
| PpsI | GAGTC | 2 cut(s) | 1649, 1813 |
| Ppu21I | YACGTR | 1 cut(s) | 1690 |
| PpuMI | RGGWCCY | 1 cut(s) | 1310 |
| PshAI | GACNNNNGTC | 2 cut(s) | 708, 1505 |
| PshBI | ATTAAT | 1 cut(s) | 762 |
| Psp124BI | GAGCTC | 1 cut(s) | 358 |
| Psp5II | RGGWCCY | 1 cut(s) | 1310 |
| Psp6I | CCWGG | 3 cut(s) | 337, 970, 1750 |
| PspFI | CCCAGC | 1 cut(s) | 1359 |
| PspGI | CCWGG | 3 cut(s) | 337, 970, 1750 |
| PspN4I | GGNNCC | 4 cut(s) | 592, 928, 1312, 1573 |
| PspPI | GGNCC | 4 cut(s) | 19, 341, 461, 1310 |
| PspPPI | RGGWCCY | 1 cut(s) | 1310 |
| PspXI | VCTCGAGB | 1 cut(s) | 240 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 490, 522 |
| PstNI | CAGNNNCTG | 1 cut(s) | 190 |
| PsuI | RGATCY | 3 cut(s) | 50, 668, 1477 |
| PteI | GCGCGC | 1 cut(s) | 125 |
| PvuI | CGATCG | 1 cut(s) | 1431 |
| PvuII | CAGCTG | 1 cut(s) | 749 |
| RsaI | GTAC | 3 cut(s) | 157, 1265, 1297 |
| RsaNI | GTAC | 3 cut(s) | 156, 1264, 1296 |
| RseI | CAYNNNNRTG | 2 cut(s) | 423, 1596 |
| SacI | GAGCTC | 1 cut(s) | 358 |
| SalI | GTCGAC | 1 cut(s) | 178 |
| SapI | GCTCTTC | 1 cut(s) | 230 |
| SaqAI | TTAA | 8 cut(s) | 104, 273, 381, 762, 1449, 1487, 1668, 1673 |
| SatI | GCNGC | 5 cut(s) | 130, 201, 720, 747, 1338 |
| Sau3AI | GATC | 6 cut(s) | 50, 654, 668, 1428, 1477, 1678 |
| Sau96I | GGNCC | 4 cut(s) | 19, 341, 461, 1310 |
| ScaI | AGTACT | 1 cut(s) | 157 |
| SchI | GAGTC | 2 cut(s) | 1649, 1814 |
| ScrFI | CCNGG | 4 cut(s) | 339, 495, 972, 1752 |
| SduI | GDGCHC | 2 cut(s) | 358, 1835 |
| SfaNI | GCATC | 3 cut(s) | 1069, 1076, 1503 |
| SfcI | CTRYAG | 4 cut(s) | 69, 97, 1053, 1186 |
| Sfr274I | CTCGAG | 1 cut(s) | 240 |
| SinI | GGWCC | 3 cut(s) | 19, 341, 1310 |
| SlaI | CTCGAG | 1 cut(s) | 240 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 423, 1596 |
| SmlI | CTYRAG | 2 cut(s) | 103, 240 |
| SmoI | CTYRAG | 2 cut(s) | 103, 240 |
| SnaBI | TACGTA | 1 cut(s) | 1690 |
| SpeI | ACTAGT | 1 cut(s) | 161 |
| SphI | GCATGC | 1 cut(s) | 557 |
| Sse9I | AATT | 9 cut(s) | 59, 372, 502, 685, 759, 1003, 1636, 1669, 1674 |
| SsiI | CCGC | 2 cut(s) | 221, 1278 |
| SspI | AATATT | 1 cut(s) | 1354 |
| SspMI | CTAG | 5 cut(s) | 162, 291, 573, 1475, 1517 |
| SstI | GAGCTC | 1 cut(s) | 358 |
| StyD4I | CCNGG | 4 cut(s) | 337, 493, 970, 1750 |
| StyI | CCWWGG | 1 cut(s) | 449 |
| TaaI | ACNGT | 7 cut(s) | 186, 590, 910, 1057, 1295, 1492, 1534 |
| TaiI | ACGT | 3 cut(s) | 809, 990, 1692 |
| TaqI | TCGA | 4 cut(s) | 179, 241, 867, 1431 |
| TasI | AATT | 9 cut(s) | 59, 372, 502, 685, 759, 1003, 1636, 1669, 1674 |
| TatI | WGTACW | 3 cut(s) | 155, 1263, 1295 |
| TfiI | GAWTC | 6 cut(s) | 302, 1147, 1154, 1376, 1579, 1734 |
| Tru1I | TTAA | 8 cut(s) | 104, 273, 381, 762, 1449, 1487, 1668, 1673 |
| Tru9I | TTAA | 8 cut(s) | 104, 273, 381, 762, 1449, 1487, 1668, 1673 |
| TscAI | CASTG | 6 cut(s) | 144, 177, 425, 913, 1462, 1488 |
| TseFI | GTSAC | 3 cut(s) | 472, 563, 1772 |
| TseI | GCWGC | 5 cut(s) | 129, 200, 719, 746, 1337 |
| Tsp45I | GTSAC | 3 cut(s) | 472, 563, 1772 |
| TspDTI | ATGAA | 7 cut(s) | 74, 164, 217, 242, 660, 1424, 1542 |
| TspGWI | ACGGA | 1 cut(s) | 1564 |
| TspRI | CASTG | 6 cut(s) | 144, 177, 425, 913, 1462, 1488 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 420, 1715 |
| Vha464I | CTTAAG | 1 cut(s) | 103 |
| VpaK11BI | GGWCC | 3 cut(s) | 19, 341, 1310 |
| VspI | ATTAAT | 1 cut(s) | 762 |
| XapI | RAATTY | 3 cut(s) | 502, 685, 1003 |
| XceI | RCATGY | 2 cut(s) | 557, 1320 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 447, 1716 |
| XhoI | CTCGAG | 1 cut(s) | 240 |
| XmiI | GTMKAC | 1 cut(s) | 179 |
| XmnI | GAANNNNTTC | 2 cut(s) | 1146, 1640 |
| XspI | CTAG | 5 cut(s) | 162, 291, 573, 1475, 1517 |
| ZrmI | AGTACT | 1 cut(s) | 157 |