Lignin degradation and detoxification of lignin-derived products
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 1545, 1580 |
| AccB1I | GGYRCC | 1 cut(s) | 452 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 282 |
| AccBSI | CCGCTC | 1 cut(s) | 85 |
| AccI | GTMKAC | 1 cut(s) | 41 |
| AciI | CCGC | 2 cut(s) | 83, 1146 |
| AclI | AACGTT | 1 cut(s) | 1574 |
| AclWI | GGATC | 2 cut(s) | 538, 1550 |
| AcoI | YGGCCR | 1 cut(s) | 628 |
| AcsI | RAATTY | 4 cut(s) | 364, 547, 865, 1014 |
| AfaI | GTAC | 4 cut(s) | 19, 33, 1133, 1165 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 282, 476, 772, 1175, 1266, 1592 |
| AflIII | ACRYGT | 1 cut(s) | 849 |
| AgsI | TTSAA | 4 cut(s) | 553, 965, 1048, 1427 |
| AhlI | ACTAGT | 1 cut(s) | 23 |
| AjiI | CACGTC | 1 cut(s) | 850 |
| AjnI | CCWGG | 3 cut(s) | 199, 832, 1627 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 472, 504 |
| Alw21I | GWGCWC | 1 cut(s) | 220 |
| Alw26I | GTCTC | 3 cut(s) | 1524, 1654, 1712 |
| AlwI | GGATC | 2 cut(s) | 538, 1550 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 52, 932 |
| Ama87I | CYCGRG | 1 cut(s) | 102 |
| AoxI | GGCC | 2 cut(s) | 322, 628 |
| ApeKI | GCWGC | 3 cut(s) | 62, 608, 1205 |
| ApoI | RAATTY | 4 cut(s) | 364, 547, 865, 1014 |
| AseI | ATTAAT | 1 cut(s) | 624 |
| AspS9I | GGNCC | 5 cut(s) | 203, 323, 577, 1178, 1733 |
| AsuC2I | CCSGG | 1 cut(s) | 357 |
| AsuHPI | GGTGA | 2 cut(s) | 265, 345 |
| AvaI | CYCGRG | 1 cut(s) | 102 |
| AvaII | GGWCC | 4 cut(s) | 203, 577, 1178, 1733 |
| BaeGI | GKGCMC | 1 cut(s) | 1712 |
| BanI | GGYRCC | 1 cut(s) | 452 |
| BanII | GRGCYC | 1 cut(s) | 220 |
| Bbv12I | GWGCWC | 1 cut(s) | 220 |
| BbvI | GCAGC | 3 cut(s) | 49, 620, 1192 |
| BccI | CCATC | 6 cut(s) | 314, 812, 1411, 1435, 1590, 1613 |
| BceAI | ACGGC | 1 cut(s) | 802 |
| BciT130I | CCWGG | 3 cut(s) | 201, 834, 1629 |
| BciVI | GTATCC | 2 cut(s) | 223, 1098 |
| BcnI | CCSGG | 1 cut(s) | 357 |
| BcoDI | GTCTC | 3 cut(s) | 1524, 1654, 1712 |
| BcuI | ACTAGT | 1 cut(s) | 23 |
| BfaI | CTAG | 5 cut(s) | 24, 153, 435, 1352, 1394 |
| BfmI | CTRYAG | 2 cut(s) | 915, 1264 |
| BfuI | GTATCC | 2 cut(s) | 223, 1098 |
| BisI | GCNGC | 3 cut(s) | 63, 609, 1206 |
| BlpI | GCTNAGC | 1 cut(s) | 1072 |
| BlsI | GCNGC | 3 cut(s) | 64, 610, 1207 |
| BmcAI | AGTACT | 2 cut(s) | 19, 33 |
| Bme1390I | CCNGG | 4 cut(s) | 201, 357, 834, 1629 |
| Bme18I | GGWCC | 4 cut(s) | 203, 577, 1178, 1733 |
| BmeT110I | CYCGRG | 1 cut(s) | 102 |
| BmgBI | CACGTC | 1 cut(s) | 850 |
| BmgT120I | GGNCC | 5 cut(s) | 203, 323, 577, 1178, 1733 |
| BmiI | GGNNCC | 5 cut(s) | 454, 579, 790, 1180, 1450 |
| BmrFI | CCNGG | 4 cut(s) | 201, 357, 834, 1629 |
| BmrI | ACTGGG | 1 cut(s) | 1014 |
| BmsI | GCATC | 2 cut(s) | 937, 1030 |
| BmuI | ACTGGG | 1 cut(s) | 1014 |
| BoxI | GACNNNNGTC | 2 cut(s) | 570, 1382 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1517, 1549 |
| BpmI | CTGGAG | 2 cut(s) | 1152, 1722 |
| Bpu10I | CCTNAGC | 1 cut(s) | 498 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1072 |
| BpuMI | CCSGG | 1 cut(s) | 357 |
| Bsa29I | ATCGAT | 1 cut(s) | 729 |
| BsaAI | YACGTR | 1 cut(s) | 1567 |
| BsaJI | CCNNGG | 3 cut(s) | 311, 832, 1372 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 282, 476, 772, 1175, 1266, 1592 |
| Bse118I | RCCGGY | 2 cut(s) | 630, 1282 |
| Bse1I | ACTGG | 4 cut(s) | 29, 287, 434, 1020 |
| Bse3DI | GCAATG | 1 cut(s) | 750 |
| BseBI | CCWGG | 3 cut(s) | 201, 834, 1629 |
| BseCI | ATCGAT | 1 cut(s) | 729 |
| BseDI | CCNNGG | 3 cut(s) | 311, 832, 1372 |
| BseGI | GGATG | 3 cut(s) | 75, 1459, 1601 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 282, 476, 772, 1175, 1266, 1592 |
| BseMI | GCAATG | 1 cut(s) | 750 |
| BseMII | CTCAG | 2 cut(s) | 489, 1707 |
| BseNI | ACTGG | 4 cut(s) | 29, 287, 434, 1020 |
| BseRI | GAGGAG | 1 cut(s) | 527 |
| BseSI | GKGCMC | 1 cut(s) | 1712 |
| BseXI | GCAGC | 3 cut(s) | 49, 620, 1192 |
| BshFI | GGCC | 2 cut(s) | 324, 630 |
| BshNI | GGYRCC | 1 cut(s) | 452 |
| BshVI | ATCGAT | 1 cut(s) | 729 |
| BsiHKAI | GWGCWC | 1 cut(s) | 220 |
| BsiHKCI | CYCGRG | 1 cut(s) | 102 |
| BsiSI | CCGG | 3 cut(s) | 356, 631, 1283 |
| BslFI | GGGAC | 3 cut(s) | 1085, 1164, 1390 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 282, 476, 772, 1175, 1266, 1592 |
| BsmAI | GTCTC | 3 cut(s) | 1524, 1654, 1712 |
| BsmFI | GGGAC | 3 cut(s) | 1085, 1164, 1390 |
| BsmI | GAATGC | 1 cut(s) | 1043 |
| BsnI | GGCC | 2 cut(s) | 324, 630 |
| BsoBI | CYCGRG | 1 cut(s) | 102 |
| Bsp1286I | GDGCHC | 2 cut(s) | 220, 1712 |
| Bsp1407I | TGTACA | 1 cut(s) | 1131 |
| Bsp143I | GATC | 4 cut(s) | 516, 530, 1354, 1555 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1072 |
| Bsp19I | CCATGG | 1 cut(s) | 311 |
| BspACI | CCGC | 2 cut(s) | 83, 1146 |
| BspANI | GGCC | 2 cut(s) | 324, 630 |
| BspCNI | CTCAG | 2 cut(s) | 490, 1708 |
| BspDI | ATCGAT | 1 cut(s) | 729 |
| BspLI | GGNNCC | 5 cut(s) | 454, 579, 790, 1180, 1450 |
| BspPI | GGATC | 2 cut(s) | 538, 1550 |
| BspQI | GCTCTTC | 1 cut(s) | 92 |
| BspT107I | GGYRCC | 1 cut(s) | 452 |
| BsrBI | CCGCTC | 1 cut(s) | 85 |
| BsrDI | GCAATG | 1 cut(s) | 750 |
| BsrFI | RCCGGY | 2 cut(s) | 630, 1282 |
| BsrGI | TGTACA | 1 cut(s) | 1131 |
| BsrI | ACTGG | 4 cut(s) | 29, 287, 434, 1020 |
| BssAI | RCCGGY | 2 cut(s) | 630, 1282 |
| BssECI | CCNNGG | 3 cut(s) | 311, 832, 1372 |
| BssMI | GATC | 4 cut(s) | 516, 530, 1354, 1555 |
| BssT1I | CCWWGG | 2 cut(s) | 311, 1372 |
| Bst2UI | CCWGG | 3 cut(s) | 201, 834, 1629 |
| Bst4CI | ACNGT | 7 cut(s) | 48, 452, 772, 919, 928, 1369, 1411 |
| Bst6I | CTCTTC | 4 cut(s) | 8, 92, 504, 892 |
| BstAUI | TGTACA | 1 cut(s) | 1131 |
| BstBAI | YACGTR | 1 cut(s) | 1567 |
| BstC8I | GCNNGC | 7 cut(s) | 220, 417, 442, 471, 632, 1210, 1280 |
| BstDEI | CTNAG | 4 cut(s) | 498, 1072, 1716, 1787 |
| BstDSI | CCRYGG | 1 cut(s) | 311 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1264 |
| BstF5I | GGATG | 3 cut(s) | 75, 1459, 1601 |
| BstKTI | GATC | 4 cut(s) | 519, 533, 1357, 1558 |
| BstMAI | GTCTC | 3 cut(s) | 1524, 1654, 1712 |
| BstMBI | GATC | 4 cut(s) | 516, 530, 1354, 1555 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 215, 786, 947 |
| BstNI | CCWGG | 3 cut(s) | 201, 834, 1629 |
| BstNSI | RCATGY | 1 cut(s) | 419 |
| BstPAI | GACNNNNGTC | 2 cut(s) | 570, 1382 |
| BstSCI | CCNGG | 4 cut(s) | 199, 355, 832, 1627 |
| BstSFI | CTRYAG | 2 cut(s) | 915, 1264 |
| BstSLI | GKGCMC | 1 cut(s) | 1712 |
| BstSNI | TACGTA | 1 cut(s) | 1567 |
| BstV1I | GCAGC | 3 cut(s) | 49, 620, 1192 |
| BstX2I | RGATCY | 1 cut(s) | 530 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1635 |
| BstYI | RGATCY | 1 cut(s) | 530 |
| Bsu15I | ATCGAT | 1 cut(s) | 729 |
| BsuI | GTATCC | 2 cut(s) | 223, 1098 |
| BsuRI | GGCC | 2 cut(s) | 324, 630 |
| BsuTUI | ATCGAT | 1 cut(s) | 729 |
| BtgI | CCRYGG | 1 cut(s) | 311 |
| BtgZI | GCGATG | 1 cut(s) | 1358 |
| BtrI | CACGTC | 1 cut(s) | 850 |
| BtsCI | GGATG | 3 cut(s) | 75, 1459, 1601 |
| BtsIMutI | CAGTG | 3 cut(s) | 280, 768, 1332 |
| Cac8I | GCNNGC | 7 cut(s) | 220, 417, 442, 471, 632, 1210, 1280 |
| CaiI | CAGNNNCTG | 2 cut(s) | 52, 932 |
| Cfr10I | RCCGGY | 2 cut(s) | 630, 1282 |
| Cfr13I | GGNCC | 5 cut(s) | 203, 323, 577, 1178, 1733 |
| ClaI | ATCGAT | 1 cut(s) | 729 |
| CseI | GACGC | 1 cut(s) | 1201 |
| Csp6I | GTAC | 4 cut(s) | 18, 32, 1132, 1164 |
| CviQI | GTAC | 4 cut(s) | 18, 32, 1132, 1164 |
| DdeI | CTNAG | 4 cut(s) | 498, 1072, 1716, 1787 |
| DpnI | GATC | 4 cut(s) | 518, 532, 1356, 1557 |
| DpnII | GATC | 4 cut(s) | 516, 530, 1354, 1555 |
| EaeI | YGGCCR | 1 cut(s) | 628 |
| Eam1104I | CTCTTC | 4 cut(s) | 8, 92, 504, 892 |
| EarI | CTCTTC | 4 cut(s) | 8, 92, 504, 892 |
| EciI | GGCGGA | 1 cut(s) | 1135 |
| Ecl136II | GAGCTC | 1 cut(s) | 218 |
| Eco105I | TACGTA | 1 cut(s) | 1567 |
| Eco130I | CCWWGG | 2 cut(s) | 311, 1372 |
| Eco24I | GRGCYC | 1 cut(s) | 220 |
| Eco47I | GGWCC | 4 cut(s) | 203, 577, 1178, 1733 |
| Eco53kI | GAGCTC | 1 cut(s) | 218 |
| Eco88I | CYCGRG | 1 cut(s) | 102 |
| EcoICRI | GAGCTC | 1 cut(s) | 218 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1264 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1178 |
| EcoRII | CCWGG | 3 cut(s) | 199, 832, 1627 |
| EcoT14I | CCWWGG | 2 cut(s) | 311, 1372 |
| EcoT22I | ATGCAT | 1 cut(s) | 1045 |
| EcoT38I | GRGCYC | 1 cut(s) | 220 |
| ErhI | CCWWGG | 2 cut(s) | 311, 1372 |
| FaqI | GGGAC | 3 cut(s) | 1085, 1164, 1390 |
| FblI | GTMKAC | 1 cut(s) | 41 |
| Fnu4HI | GCNGC | 3 cut(s) | 63, 609, 1206 |
| FokI | GGATG | 3 cut(s) | 62, 1446, 1608 |
| FriOI | GRGCYC | 1 cut(s) | 220 |
| Fsp4HI | GCNGC | 3 cut(s) | 63, 609, 1206 |
| FspBI | CTAG | 5 cut(s) | 24, 153, 435, 1352, 1394 |
| GluI | GCNGC | 3 cut(s) | 63, 609, 1206 |
| GsuI | CTGGAG | 2 cut(s) | 1152, 1722 |
| HaeIII | GGCC | 2 cut(s) | 324, 630 |
| HapII | CCGG | 3 cut(s) | 356, 631, 1283 |
| HgaI | GACGC | 1 cut(s) | 1201 |
| HincII | GTYRAC | 6 cut(s) | 42, 387, 565, 922, 1365, 1602 |
| HindII | GTYRAC | 6 cut(s) | 42, 387, 565, 922, 1365, 1602 |
| HindIII | AAGCTT | 1 cut(s) | 1069 |
| HinfI | GANTC | 8 cut(s) | 164, 935, 1244, 1456, 1532, 1682, 1772, 1810 |
| HpaI | GTTAAC | 1 cut(s) | 1602 |
| HpaII | CCGG | 3 cut(s) | 356, 631, 1283 |
| HphI | GGTGA | 2 cut(s) | 265, 345 |
| Hpy188I | TCNGA | 7 cut(s) | 530, 711, 896, 934, 991, 1239, 1610 |
| Hpy188III | TCNNGA | 4 cut(s) | 497, 575, 1169, 1304 |
| Hpy99I | CGWCG | 1 cut(s) | 678 |
| HpyAV | CCTTC | 2 cut(s) | 392, 1761 |
| HpyCH4III | ACNGT | 7 cut(s) | 48, 452, 772, 919, 928, 1369, 1411 |
| HpyCH4IV | ACGT | 4 cut(s) | 668, 849, 1566, 1574 |
| HpyCH4V | TGCA | 6 cut(s) | 584, 956, 1043, 1208, 1468, 1474 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 215, 786, 947 |
| HpyF3I | CTNAG | 4 cut(s) | 498, 1072, 1716, 1787 |
| HpySE526I | ACGT | 4 cut(s) | 668, 849, 1566, 1574 |
| KroI | GCCGGC | 1 cut(s) | 630 |
| KroNI | GCCGGC | 1 cut(s) | 632 |
| KspAI | GTTAAC | 1 cut(s) | 1602 |
| Kzo9I | GATC | 4 cut(s) | 516, 530, 1354, 1555 |
| LguI | GCTCTTC | 1 cut(s) | 92 |
| LmnI | GCTCC | 4 cut(s) | 659, 794, 879, 1000 |
| Lsp1109I | GCAGC | 3 cut(s) | 49, 620, 1192 |
| LweI | GCATC | 2 cut(s) | 937, 1030 |
| MaeI | CTAG | 5 cut(s) | 24, 153, 435, 1352, 1394 |
| MaeII | ACGT | 4 cut(s) | 668, 849, 1566, 1574 |
| MaeIII | GTNAC | 7 cut(s) | 334, 425, 614, 974, 1567, 1649, 1790 |
| MalI | GATC | 4 cut(s) | 518, 532, 1356, 1557 |
| MbiI | CCGCTC | 1 cut(s) | 85 |
| MboI | GATC | 4 cut(s) | 516, 530, 1354, 1555 |
| MboII | GAAGA | 8 cut(s) | 25, 79, 407, 521, 695, 698, 909, 1711 |
| MflI | RGATCY | 1 cut(s) | 530 |
| MhlI | GDGCHC | 2 cut(s) | 220, 1712 |
| MluCI | AATT | 8 cut(s) | 234, 364, 547, 621, 865, 1014, 1546, 1799 |
| MlyI | GAGTC | 3 cut(s) | 929, 1526, 1691 |
| MmeI | TCCRAC | 1 cut(s) | 874 |
| MnlI | CCTC | 6 cut(s) | 133, 505, 776, 1192, 1569, 1746 |
| Mph1103I | ATGCAT | 1 cut(s) | 1045 |
| MroNI | GCCGGC | 1 cut(s) | 630 |
| MseI | TTAA | 6 cut(s) | 135, 243, 624, 1326, 1601, 1806 |
| MslI | CAYNNNNRTG | 2 cut(s) | 285, 1473 |
| MspA1I | CMGCKG | 1 cut(s) | 611 |
| MspI | CCGG | 3 cut(s) | 356, 631, 1283 |
| MspR9I | CCNGG | 4 cut(s) | 201, 357, 834, 1629 |
| Mva1269I | GAATGC | 1 cut(s) | 1043 |
| MvaI | CCWGG | 3 cut(s) | 201, 834, 1629 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 215, 786, 947 |
| NaeI | GCCGGC | 1 cut(s) | 632 |
| NciI | CCSGG | 1 cut(s) | 357 |
| NcoI | CCATGG | 1 cut(s) | 311 |
| NdeII | GATC | 4 cut(s) | 516, 530, 1354, 1555 |
| NgoMIV | GCCGGC | 1 cut(s) | 630 |
| NlaIV | GGNNCC | 5 cut(s) | 454, 579, 790, 1180, 1450 |
| NmuCI | GTSAC | 4 cut(s) | 334, 425, 1649, 1790 |
| NsiI | ATGCAT | 1 cut(s) | 1045 |
| NspI | RCATGY | 1 cut(s) | 419 |
| PaeI | GCATGC | 1 cut(s) | 419 |
| PaeR7I | CTCGAG | 1 cut(s) | 102 |
| PciSI | GCTCTTC | 1 cut(s) | 92 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 726 |
| PctI | GAATGC | 1 cut(s) | 1043 |
| PdiI | GCCGGC | 1 cut(s) | 632 |
| PfeI | GAWTC | 5 cut(s) | 164, 1244, 1456, 1772, 1810 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 282 |
| PkrI | GCNGC | 3 cut(s) | 64, 610, 1207 |
| PleI | GAGTC | 3 cut(s) | 929, 1526, 1690 |
| PpsI | GAGTC | 3 cut(s) | 929, 1526, 1690 |
| Ppu21I | YACGTR | 1 cut(s) | 1567 |
| PpuMI | RGGWCCY | 1 cut(s) | 1178 |
| PshAI | GACNNNNGTC | 2 cut(s) | 570, 1382 |
| PshBI | ATTAAT | 1 cut(s) | 624 |
| PsiI | TTATAA | 2 cut(s) | 1545, 1580 |
| Psp124BI | GAGCTC | 1 cut(s) | 220 |
| Psp1406I | AACGTT | 1 cut(s) | 1574 |
| Psp5II | RGGWCCY | 1 cut(s) | 1178 |
| Psp6I | CCWGG | 3 cut(s) | 199, 832, 1627 |
| PspGI | CCWGG | 3 cut(s) | 199, 832, 1627 |
| PspN4I | GGNNCC | 5 cut(s) | 454, 579, 790, 1180, 1450 |
| PspPI | GGNCC | 5 cut(s) | 203, 323, 577, 1178, 1733 |
| PspPPI | RGGWCCY | 1 cut(s) | 1178 |
| PspXI | VCTCGAGB | 1 cut(s) | 102 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 352, 384 |
| PstNI | CAGNNNCTG | 2 cut(s) | 52, 932 |
| PsuI | RGATCY | 1 cut(s) | 530 |
| PvuII | CAGCTG | 1 cut(s) | 611 |
| RsaI | GTAC | 4 cut(s) | 19, 33, 1133, 1165 |
| RsaNI | GTAC | 4 cut(s) | 18, 32, 1132, 1164 |
| RseI | CAYNNNNRTG | 2 cut(s) | 285, 1473 |
| SacI | GAGCTC | 1 cut(s) | 220 |
| SalI | GTCGAC | 1 cut(s) | 40 |
| SapI | GCTCTTC | 1 cut(s) | 92 |
| SaqAI | TTAA | 6 cut(s) | 135, 243, 624, 1326, 1601, 1806 |
| SatI | GCNGC | 3 cut(s) | 63, 609, 1206 |
| Sau3AI | GATC | 4 cut(s) | 516, 530, 1354, 1555 |
| Sau96I | GGNCC | 5 cut(s) | 203, 323, 577, 1178, 1733 |
| ScaI | AGTACT | 2 cut(s) | 19, 33 |
| SchI | GAGTC | 3 cut(s) | 929, 1526, 1691 |
| ScrFI | CCNGG | 4 cut(s) | 201, 357, 834, 1629 |
| SduI | GDGCHC | 2 cut(s) | 220, 1712 |
| SfaNI | GCATC | 2 cut(s) | 937, 1030 |
| SfcI | CTRYAG | 2 cut(s) | 915, 1264 |
| Sfr274I | CTCGAG | 1 cut(s) | 102 |
| SinI | GGWCC | 4 cut(s) | 203, 577, 1178, 1733 |
| SlaI | CTCGAG | 1 cut(s) | 102 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 285, 1473 |
| SmlI | CTYRAG | 1 cut(s) | 102 |
| SmoI | CTYRAG | 1 cut(s) | 102 |
| SnaBI | TACGTA | 1 cut(s) | 1567 |
| SpeI | ACTAGT | 1 cut(s) | 23 |
| SphI | GCATGC | 1 cut(s) | 419 |
| Sse9I | AATT | 8 cut(s) | 234, 364, 547, 621, 865, 1014, 1546, 1799 |
| SsiI | CCGC | 2 cut(s) | 83, 1146 |
| SspI | AATATT | 1 cut(s) | 1063 |
| SspMI | CTAG | 5 cut(s) | 24, 153, 435, 1352, 1394 |
| SstI | GAGCTC | 1 cut(s) | 220 |
| StyD4I | CCNGG | 4 cut(s) | 199, 355, 832, 1627 |
| StyI | CCWWGG | 2 cut(s) | 311, 1372 |
| TaaI | ACNGT | 7 cut(s) | 48, 452, 772, 919, 928, 1369, 1411 |
| TaiI | ACGT | 4 cut(s) | 671, 852, 1569, 1577 |
| TaqI | TCGA | 4 cut(s) | 41, 103, 729, 1357 |
| TaqII | GACCGA | 1 cut(s) | 1721 |
| TasI | AATT | 8 cut(s) | 234, 364, 547, 621, 865, 1014, 1546, 1799 |
| TatI | WGTACW | 4 cut(s) | 17, 31, 1131, 1163 |
| TfiI | GAWTC | 5 cut(s) | 164, 1244, 1456, 1772, 1810 |
| Tru1I | TTAA | 6 cut(s) | 135, 243, 624, 1326, 1601, 1806 |
| Tru9I | TTAA | 6 cut(s) | 135, 243, 624, 1326, 1601, 1806 |
| TscAI | CASTG | 3 cut(s) | 287, 775, 1339 |
| TseFI | GTSAC | 4 cut(s) | 334, 425, 1649, 1790 |
| TseI | GCWGC | 3 cut(s) | 62, 608, 1205 |
| Tsp45I | GTSAC | 4 cut(s) | 334, 425, 1649, 1790 |
| TspDTI | ATGAA | 7 cut(s) | 17, 26, 79, 104, 522, 1305, 1419 |
| TspGWI | ACGGA | 3 cut(s) | 1071, 1441, 1608 |
| TspRI | CASTG | 3 cut(s) | 287, 775, 1339 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 282 |
| VpaK11BI | GGWCC | 4 cut(s) | 203, 577, 1178, 1733 |
| VspI | ATTAAT | 1 cut(s) | 624 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1264 |
| XapI | RAATTY | 4 cut(s) | 364, 547, 865, 1014 |
| XceI | RCATGY | 1 cut(s) | 419 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 309 |
| XhoI | CTCGAG | 1 cut(s) | 102 |
| XmiI | GTMKAC | 1 cut(s) | 41 |
| XspI | CTAG | 5 cut(s) | 24, 153, 435, 1352, 1394 |
| ZrmI | AGTACT | 2 cut(s) | 19, 33 |
| Zsp2I | ATGCAT | 1 cut(s) | 1045 |