Lignin degradation and detoxification of lignin-derived products
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 418 |
| AccB1I | GGYRCC | 1 cut(s) | 431 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1550 |
| AccII | CGCG | 1 cut(s) | 1737 |
| AccIII | TCCGGA | 2 cut(s) | 184, 1054 |
| AciI | CCGC | 1 cut(s) | 1239 |
| AclI | AACGTT | 1 cut(s) | 1395 |
| AclWI | GGATC | 3 cut(s) | 621, 1301, 1508 |
| AcoI | YGGCCR | 3 cut(s) | 601, 850, 1560 |
| AcsI | RAATTY | 4 cut(s) | 104, 308, 1117, 1719 |
| AcuI | CTGAAG | 2 cut(s) | 906, 915 |
| AfaI | GTAC | 3 cut(s) | 518, 985, 1127 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 455, 610, 1550 |
| AgeI | ACCGGT | 1 cut(s) | 560 |
| AgsI | TTSAA | 6 cut(s) | 703, 938, 1251, 1354, 1385, 1616 |
| AjnI | CCWGG | 3 cut(s) | 178, 607, 1585 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 955, 987, 1541, 1573 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 451, 483 |
| AluBI | AGCT | 1 cut(s) | 1443 |
| AluI | AGCT | 1 cut(s) | 1443 |
| Alw26I | GTCTC | 4 cut(s) | 201, 539, 1478, 1670 |
| AlwI | GGATC | 3 cut(s) | 621, 1301, 1508 |
| Ama87I | CYCGRG | 3 cut(s) | 335, 1198, 1673 |
| Aor13HI | TCCGGA | 2 cut(s) | 184, 1054 |
| AoxI | GGCC | 5 cut(s) | 507, 601, 751, 850, 1560 |
| ApeKI | GCWGC | 2 cut(s) | 866, 1643 |
| ApoI | RAATTY | 4 cut(s) | 104, 308, 1117, 1719 |
| AsiGI | ACCGGT | 1 cut(s) | 560 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 125 |
| AspS9I | GGNCC | 2 cut(s) | 182, 302 |
| AsuC2I | CCSGG | 5 cut(s) | 336, 337, 807, 1199, 1200 |
| AsuII | TTCGAA | 1 cut(s) | 831 |
| AvaI | CYCGRG | 3 cut(s) | 335, 1198, 1673 |
| AvaII | GGWCC | 2 cut(s) | 182, 302 |
| BaeGI | GKGCMC | 1 cut(s) | 1670 |
| BalI | TGGCCA | 3 cut(s) | 603, 852, 1562 |
| BanI | GGYRCC | 1 cut(s) | 431 |
| BbsI | GAAGAC | 1 cut(s) | 706 |
| BbvI | GCAGC | 2 cut(s) | 853, 1630 |
| BccI | CCATC | 5 cut(s) | 293, 752, 1548, 1571, 1698 |
| BceAI | ACGGC | 3 cut(s) | 564, 633, 1450 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 142, 176 |
| BciT130I | CCWGG | 3 cut(s) | 180, 609, 1587 |
| BcnI | CCSGG | 5 cut(s) | 336, 337, 807, 1199, 1200 |
| BcoDI | GTCTC | 4 cut(s) | 201, 539, 1478, 1670 |
| BfaI | CTAG | 4 cut(s) | 132, 1046, 1400, 1680 |
| BfmI | CTRYAG | 2 cut(s) | 1225, 1438 |
| BisI | GCNGC | 2 cut(s) | 867, 1644 |
| BlsI | GCNGC | 2 cut(s) | 868, 1645 |
| Bme1390I | CCNGG | 8 cut(s) | 180, 336, 337, 609, 807, 1199, 1200, 1587 |
| Bme18I | GGWCC | 2 cut(s) | 182, 302 |
| BmeT110I | CYCGRG | 3 cut(s) | 335, 1198, 1673 |
| BmgT120I | GGNCC | 2 cut(s) | 182, 302 |
| BmiI | GGNNCC | 3 cut(s) | 433, 460, 763 |
| BmrFI | CCNGG | 8 cut(s) | 180, 336, 337, 609, 807, 1199, 1200, 1587 |
| BmsI | GCATC | 4 cut(s) | 103, 574, 880, 1741 |
| BpiI | GAAGAC | 1 cut(s) | 706 |
| Bpu14I | TTCGAA | 1 cut(s) | 831 |
| BpuEI | CTTGAG | 2 cut(s) | 1506, 1645 |
| BpuMI | CCSGG | 5 cut(s) | 336, 337, 807, 1199, 1200 |
| BsaAI | YACGTR | 1 cut(s) | 1450 |
| BsaJI | CCNNGG | 5 cut(s) | 235, 290, 335, 608, 1198 |
| BsaWI | WCCGGW | 3 cut(s) | 184, 560, 1054 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 7, 37, 198, 228 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 455, 610, 1550 |
| Bse118I | RCCGGY | 1 cut(s) | 560 |
| Bse1I | ACTGG | 2 cut(s) | 266, 630 |
| BseAI | TCCGGA | 2 cut(s) | 184, 1054 |
| BseBI | CCWGG | 3 cut(s) | 180, 609, 1587 |
| BseDI | CCNNGG | 5 cut(s) | 235, 290, 335, 608, 1198 |
| BseGI | GGATG | 5 cut(s) | 826, 1148, 1417, 1559, 1690 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 455, 610, 1550 |
| BseNI | ACTGG | 2 cut(s) | 266, 630 |
| BseRI | GAGGAG | 1 cut(s) | 1509 |
| BseSI | GKGCMC | 1 cut(s) | 1670 |
| BseXI | GCAGC | 2 cut(s) | 853, 1630 |
| Bsh1236I | CGCG | 1 cut(s) | 1737 |
| BshFI | GGCC | 5 cut(s) | 509, 603, 753, 852, 1562 |
| BshNI | GGYRCC | 1 cut(s) | 431 |
| BshTI | ACCGGT | 1 cut(s) | 560 |
| BsiHKCI | CYCGRG | 3 cut(s) | 335, 1198, 1673 |
| BsiSI | CCGG | 6 cut(s) | 185, 336, 561, 807, 1055, 1199 |
| BslFI | GGGAC | 1 cut(s) | 352 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 455, 610, 1550 |
| BsmAI | GTCTC | 4 cut(s) | 201, 539, 1478, 1670 |
| BsmBI | CGTCTC | 1 cut(s) | 539 |
| BsmFI | GGGAC | 1 cut(s) | 352 |
| BsmI | GAATGC | 3 cut(s) | 697, 709, 1683 |
| BsnI | GGCC | 5 cut(s) | 509, 603, 753, 852, 1562 |
| BsoBI | CYCGRG | 3 cut(s) | 335, 1198, 1673 |
| Bsp119I | TTCGAA | 1 cut(s) | 831 |
| Bsp1286I | GDGCHC | 1 cut(s) | 1670 |
| Bsp13I | TCCGGA | 2 cut(s) | 184, 1054 |
| Bsp143I | GATC | 5 cut(s) | 495, 613, 930, 1306, 1513 |
| Bsp19I | CCATGG | 1 cut(s) | 290 |
| BspACI | CCGC | 1 cut(s) | 1239 |
| BspANI | GGCC | 5 cut(s) | 509, 603, 753, 852, 1562 |
| BspEI | TCCGGA | 2 cut(s) | 184, 1054 |
| BspFNI | CGCG | 1 cut(s) | 1737 |
| BspLI | GGNNCC | 3 cut(s) | 433, 460, 763 |
| BspPI | GGATC | 3 cut(s) | 621, 1301, 1508 |
| BspT104I | TTCGAA | 1 cut(s) | 831 |
| BspT107I | GGYRCC | 1 cut(s) | 431 |
| BsrFI | RCCGGY | 1 cut(s) | 560 |
| BsrI | ACTGG | 2 cut(s) | 266, 630 |
| BssAI | RCCGGY | 1 cut(s) | 560 |
| BssECI | CCNNGG | 5 cut(s) | 235, 290, 335, 608, 1198 |
| BssMI | GATC | 5 cut(s) | 495, 613, 930, 1306, 1513 |
| BssT1I | CCWWGG | 2 cut(s) | 235, 290 |
| Bst2UI | CCWGG | 3 cut(s) | 180, 609, 1587 |
| Bst4CI | ACNGT | 4 cut(s) | 214, 431, 745, 1130 |
| Bst6I | CTCTTC | 2 cut(s) | 71, 583 |
| BstBAI | YACGTR | 1 cut(s) | 1450 |
| BstBI | TTCGAA | 1 cut(s) | 831 |
| BstC8I | GCNNGC | 6 cut(s) | 155, 396, 450, 534, 605, 1218 |
| BstDSI | CCRYGG | 1 cut(s) | 290 |
| BstF5I | GGATG | 5 cut(s) | 826, 1148, 1417, 1559, 1690 |
| BstFNI | CGCG | 1 cut(s) | 1737 |
| BstKTI | GATC | 5 cut(s) | 498, 616, 933, 1309, 1516 |
| BstMAI | GTCTC | 4 cut(s) | 201, 539, 1478, 1670 |
| BstMBI | GATC | 5 cut(s) | 495, 613, 930, 1306, 1513 |
| BstNI | CCWGG | 3 cut(s) | 180, 609, 1587 |
| BstNSI | RCATGY | 2 cut(s) | 157, 398 |
| BstSCI | CCNGG | 8 cut(s) | 178, 334, 335, 607, 805, 1197, 1198, 1585 |
| BstSFI | CTRYAG | 2 cut(s) | 1225, 1438 |
| BstSLI | GKGCMC | 1 cut(s) | 1670 |
| BstUI | CGCG | 1 cut(s) | 1737 |
| BstV1I | GCAGC | 2 cut(s) | 853, 1630 |
| BstV2I | GAAGAC | 1 cut(s) | 706 |
| BstX2I | RGATCY | 1 cut(s) | 613 |
| BstYI | RGATCY | 1 cut(s) | 613 |
| BsuRI | GGCC | 5 cut(s) | 509, 603, 753, 852, 1562 |
| BtgI | CCRYGG | 1 cut(s) | 290 |
| BtsCI | GGATG | 5 cut(s) | 826, 1148, 1417, 1559, 1690 |
| BtsI | GCAGTG | 1 cut(s) | 1113 |
| BtsIMutI | CAGTG | 3 cut(s) | 259, 637, 1113 |
| Cac8I | GCNNGC | 6 cut(s) | 155, 396, 450, 534, 605, 1218 |
| Cfr10I | RCCGGY | 1 cut(s) | 560 |
| Cfr13I | GGNCC | 2 cut(s) | 182, 302 |
| Cfr9I | CCCGGG | 2 cut(s) | 335, 1198 |
| CseI | GACGC | 3 cut(s) | 408, 1649, 1726 |
| Csp6I | GTAC | 3 cut(s) | 517, 984, 1126 |
| CspAI | ACCGGT | 1 cut(s) | 560 |
| CviQI | GTAC | 3 cut(s) | 517, 984, 1126 |
| DpnI | GATC | 5 cut(s) | 497, 615, 932, 1308, 1515 |
| DpnII | GATC | 5 cut(s) | 495, 613, 930, 1306, 1513 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 418 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 418 |
| EaeI | YGGCCR | 3 cut(s) | 601, 850, 1560 |
| Eam1104I | CTCTTC | 2 cut(s) | 71, 583 |
| EarI | CTCTTC | 2 cut(s) | 71, 583 |
| Eco130I | CCWWGG | 2 cut(s) | 235, 290 |
| Eco147I | AGGCCT | 1 cut(s) | 753 |
| Eco32I | GATATC | 1 cut(s) | 6 |
| Eco47I | GGWCC | 2 cut(s) | 182, 302 |
| Eco57I | CTGAAG | 2 cut(s) | 906, 915 |
| Eco88I | CYCGRG | 3 cut(s) | 335, 1198, 1673 |
| EcoRI | GAATTC | 1 cut(s) | 1117 |
| EcoRII | CCWGG | 3 cut(s) | 178, 607, 1585 |
| EcoRV | GATATC | 1 cut(s) | 6 |
| EcoT14I | CCWWGG | 2 cut(s) | 235, 290 |
| EcoT22I | ATGCAT | 1 cut(s) | 1428 |
| ErhI | CCWWGG | 2 cut(s) | 235, 290 |
| Esp3I | CGTCTC | 1 cut(s) | 539 |
| FaqI | GGGAC | 1 cut(s) | 352 |
| Fnu4HI | GCNGC | 2 cut(s) | 867, 1644 |
| FokI | GGATG | 5 cut(s) | 833, 1155, 1404, 1566, 1677 |
| Fsp4HI | GCNGC | 2 cut(s) | 867, 1644 |
| FspBI | CTAG | 4 cut(s) | 132, 1046, 1400, 1680 |
| GluI | GCNGC | 2 cut(s) | 867, 1644 |
| HaeIII | GGCC | 5 cut(s) | 509, 603, 753, 852, 1562 |
| HapII | CCGG | 6 cut(s) | 185, 336, 561, 807, 1055, 1199 |
| HgaI | GACGC | 3 cut(s) | 408, 1649, 1726 |
| HinfI | GANTC | 5 cut(s) | 1051, 1148, 1364, 1414, 1540 |
| HpaII | CCGG | 6 cut(s) | 185, 336, 561, 807, 1055, 1199 |
| Hpy166II | GTNNAC | 5 cut(s) | 166, 427, 1026, 1133, 1323 |
| Hpy188I | TCNGA | 9 cut(s) | 298, 370, 583, 873, 981, 1077, 1153, 1457, 1568 |
| Hpy188III | TCNNGA | 9 cut(s) | 9, 185, 305, 1055, 1140, 1310, 1351, 1400, 1673 |
| Hpy8I | GTNNAC | 5 cut(s) | 166, 427, 1026, 1133, 1323 |
| HpyAV | CCTTC | 4 cut(s) | 371, 981, 998, 1332 |
| HpyCH4III | ACNGT | 4 cut(s) | 214, 431, 745, 1130 |
| HpyCH4IV | ACGT | 2 cut(s) | 1395, 1449 |
| HpySE526I | ACGT | 2 cut(s) | 1395, 1449 |
| Kpn2I | TCCGGA | 2 cut(s) | 184, 1054 |
| Kzo9I | GATC | 5 cut(s) | 495, 613, 930, 1306, 1513 |
| LmnI | GCTCC | 3 cut(s) | 28, 761, 1013 |
| Lsp1109I | GCAGC | 2 cut(s) | 853, 1630 |
| LweI | GCATC | 4 cut(s) | 103, 574, 880, 1741 |
| MaeI | CTAG | 4 cut(s) | 132, 1046, 1400, 1680 |
| MaeII | ACGT | 2 cut(s) | 1395, 1449 |
| MaeIII | GTNAC | 2 cut(s) | 1297, 1525 |
| MalI | GATC | 5 cut(s) | 497, 615, 932, 1308, 1515 |
| MboI | GATC | 5 cut(s) | 495, 613, 930, 1306, 1513 |
| MflI | RGATCY | 1 cut(s) | 613 |
| MhlI | GDGCHC | 1 cut(s) | 1670 |
| MlsI | TGGCCA | 3 cut(s) | 603, 852, 1562 |
| MluNI | TGGCCA | 3 cut(s) | 603, 852, 1562 |
| MlyI | GAGTC | 2 cut(s) | 1045, 1423 |
| MmeI | TCCRAC | 3 cut(s) | 436, 986, 1435 |
| Mox20I | TGGCCA | 3 cut(s) | 603, 852, 1562 |
| Mph1103I | ATGCAT | 1 cut(s) | 1428 |
| MroI | TCCGGA | 2 cut(s) | 184, 1054 |
| MroXI | GAANNNNTTC | 1 cut(s) | 125 |
| MscI | TGGCCA | 3 cut(s) | 603, 852, 1562 |
| MseI | TTAA | 4 cut(s) | 1019, 1038, 1503, 1727 |
| MslI | CAYNNNNRTG | 3 cut(s) | 99, 264, 918 |
| Msp20I | TGGCCA | 3 cut(s) | 603, 852, 1562 |
| MspI | CCGG | 6 cut(s) | 185, 336, 561, 807, 1055, 1199 |
| MspR9I | CCNGG | 8 cut(s) | 180, 336, 337, 609, 807, 1199, 1200, 1587 |
| Mva1269I | GAATGC | 3 cut(s) | 697, 709, 1683 |
| MvaI | CCWGG | 3 cut(s) | 180, 609, 1587 |
| MvnI | CGCG | 1 cut(s) | 1737 |
| NciI | CCSGG | 5 cut(s) | 336, 337, 807, 1199, 1200 |
| NcoI | CCATGG | 1 cut(s) | 290 |
| NdeII | GATC | 5 cut(s) | 495, 613, 930, 1306, 1513 |
| NlaIV | GGNNCC | 3 cut(s) | 433, 460, 763 |
| NmeAIII | GCCGAG | 1 cut(s) | 306 |
| NsiI | ATGCAT | 1 cut(s) | 1428 |
| NspI | RCATGY | 2 cut(s) | 157, 398 |
| NspV | TTCGAA | 1 cut(s) | 831 |
| PaeI | GCATGC | 2 cut(s) | 157, 398 |
| PceI | AGGCCT | 1 cut(s) | 753 |
| PctI | GAATGC | 3 cut(s) | 697, 709, 1683 |
| PdmI | GAANNNNTTC | 1 cut(s) | 125 |
| PfeI | GAWTC | 3 cut(s) | 1148, 1364, 1540 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1550 |
| PfoI | TCCNGGA | 1 cut(s) | 805 |
| PinAI | ACCGGT | 1 cut(s) | 560 |
| PkrI | GCNGC | 2 cut(s) | 868, 1645 |
| PleI | GAGTC | 2 cut(s) | 1045, 1422 |
| PpsI | GAGTC | 2 cut(s) | 1045, 1422 |
| Ppu21I | YACGTR | 1 cut(s) | 1450 |
| Psp1406I | AACGTT | 1 cut(s) | 1395 |
| Psp6I | CCWGG | 3 cut(s) | 178, 607, 1585 |
| PspGI | CCWGG | 3 cut(s) | 178, 607, 1585 |
| PspN4I | GGNNCC | 3 cut(s) | 433, 460, 763 |
| PspPI | GGNCC | 2 cut(s) | 182, 302 |
| PsuI | RGATCY | 1 cut(s) | 613 |
| RsaI | GTAC | 3 cut(s) | 518, 985, 1127 |
| RsaNI | GTAC | 3 cut(s) | 517, 984, 1126 |
| RseI | CAYNNNNRTG | 3 cut(s) | 99, 264, 918 |
| SaqAI | TTAA | 4 cut(s) | 1019, 1038, 1503, 1727 |
| SatI | GCNGC | 2 cut(s) | 867, 1644 |
| Sau3AI | GATC | 5 cut(s) | 495, 613, 930, 1306, 1513 |
| Sau96I | GGNCC | 2 cut(s) | 182, 302 |
| SchI | GAGTC | 2 cut(s) | 1045, 1423 |
| ScrFI | CCNGG | 8 cut(s) | 180, 336, 337, 609, 807, 1199, 1200, 1587 |
| SduI | GDGCHC | 1 cut(s) | 1670 |
| SfaNI | GCATC | 4 cut(s) | 103, 574, 880, 1741 |
| SfcI | CTRYAG | 2 cut(s) | 1225, 1438 |
| SfuI | TTCGAA | 1 cut(s) | 831 |
| SinI | GGWCC | 2 cut(s) | 182, 302 |
| SmaI | CCCGGG | 2 cut(s) | 337, 1200 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 99, 264, 918 |
| SmlI | CTYRAG | 2 cut(s) | 1521, 1624 |
| SmoI | CTYRAG | 2 cut(s) | 1521, 1624 |
| SphI | GCATGC | 2 cut(s) | 157, 398 |
| SseBI | AGGCCT | 1 cut(s) | 753 |
| SsiI | CCGC | 1 cut(s) | 1239 |
| SspI | AATATT | 1 cut(s) | 857 |
| SspMI | CTAG | 4 cut(s) | 132, 1046, 1400, 1680 |
| StuI | AGGCCT | 1 cut(s) | 753 |
| StyD4I | CCNGG | 8 cut(s) | 178, 334, 335, 607, 805, 1197, 1198, 1585 |
| StyI | CCWWGG | 2 cut(s) | 235, 290 |
| TaaI | ACNGT | 4 cut(s) | 214, 431, 745, 1130 |
| TaiI | ACGT | 2 cut(s) | 1398, 1452 |
| TaqI | TCGA | 1 cut(s) | 831 |
| TatI | WGTACW | 1 cut(s) | 1125 |
| TfiI | GAWTC | 3 cut(s) | 1148, 1364, 1540 |
| Tru1I | TTAA | 4 cut(s) | 1019, 1038, 1503, 1727 |
| Tru9I | TTAA | 4 cut(s) | 1019, 1038, 1503, 1727 |
| TscAI | CASTG | 3 cut(s) | 266, 637, 1113 |
| TseI | GCWGC | 2 cut(s) | 866, 1643 |
| TspDTI | ATGAA | 7 cut(s) | 71, 117, 218, 369, 501, 1094, 1130 |
| TspMI | CCCGGG | 2 cut(s) | 335, 1198 |
| TspRI | CASTG | 3 cut(s) | 266, 637, 1113 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1550 |
| VpaK11BI | GGWCC | 2 cut(s) | 182, 302 |
| XapI | RAATTY | 4 cut(s) | 104, 308, 1117, 1719 |
| XbaI | TCTAGA | 1 cut(s) | 1399 |
| XceI | RCATGY | 2 cut(s) | 157, 398 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 242, 1551 |
| XmaI | CCCGGG | 2 cut(s) | 335, 1198 |
| XmnI | GAANNNNTTC | 1 cut(s) | 125 |
| XspI | CTAG | 4 cut(s) | 132, 1046, 1400, 1680 |
| Zsp2I | ATGCAT | 1 cut(s) | 1428 |