Lignin degradation and detoxification of lignin-derived products
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 1440 |
| Acc36I | ACCTGC | 1 cut(s) | 1440 |
| AccB1I | GGYRCC | 1 cut(s) | 419 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 249, 1553 |
| AccI | GTMKAC | 1 cut(s) | 5 |
| AccII | CGCG | 1 cut(s) | 1757 |
| AciI | CCGC | 3 cut(s) | 1107, 1274, 1757 |
| AclI | AACGTT | 1 cut(s) | 1535 |
| AclWI | GGATC | 3 cut(s) | 505, 1310, 1511 |
| AcoI | YGGCCR | 1 cut(s) | 595 |
| AcsI | RAATTY | 3 cut(s) | 331, 514, 832 |
| AcuI | CTGAAG | 1 cut(s) | 933 |
| AfaI | GTAC | 2 cut(s) | 1094, 1126 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 249, 443, 739, 1136, 1553, 1699 |
| AflIII | ACRYGT | 1 cut(s) | 816 |
| AgsI | TTSAA | 6 cut(s) | 520, 926, 1009, 1334, 1388, 1507 |
| AjiI | CACGTC | 1 cut(s) | 817 |
| AjnI | CCWGG | 3 cut(s) | 166, 799, 1588 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 439, 471 |
| Alw21I | GWGCWC | 1 cut(s) | 187 |
| Alw26I | GTCTC | 2 cut(s) | 1615, 1673 |
| AlwI | GGATC | 3 cut(s) | 505, 1310, 1511 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 899 |
| Ama87I | CYCGRG | 1 cut(s) | 69 |
| AoxI | GGCC | 2 cut(s) | 289, 595 |
| ApeKI | GCWGC | 2 cut(s) | 575, 1166 |
| ApoI | RAATTY | 3 cut(s) | 331, 514, 832 |
| AseI | ATTAAT | 2 cut(s) | 591, 1767 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 975, 1478 |
| AspS9I | GGNCC | 4 cut(s) | 170, 290, 544, 1139 |
| AsuC2I | CCSGG | 1 cut(s) | 324 |
| AsuHPI | GGTGA | 2 cut(s) | 232, 312 |
| AvaI | CYCGRG | 1 cut(s) | 69 |
| AvaII | GGWCC | 3 cut(s) | 170, 544, 1139 |
| BaeGI | GKGCMC | 1 cut(s) | 1673 |
| BanI | GGYRCC | 1 cut(s) | 419 |
| BanII | GRGCYC | 1 cut(s) | 187 |
| Bbv12I | GWGCWC | 1 cut(s) | 187 |
| BbvI | GCAGC | 2 cut(s) | 587, 1153 |
| BccI | CCATC | 5 cut(s) | 281, 779, 1372, 1396, 1551 |
| BceAI | ACGGC | 1 cut(s) | 769 |
| BciT130I | CCWGG | 3 cut(s) | 168, 801, 1590 |
| BciVI | GTATCC | 1 cut(s) | 190 |
| BcnI | CCSGG | 1 cut(s) | 324 |
| BcoDI | GTCTC | 2 cut(s) | 1615, 1673 |
| BfaI | CTAG | 4 cut(s) | 120, 402, 1313, 1355 |
| BfmI | CTRYAG | 3 cut(s) | 47, 882, 1015 |
| BfuAI | ACCTGC | 1 cut(s) | 1440 |
| BfuI | GTATCC | 1 cut(s) | 190 |
| BisI | GCNGC | 2 cut(s) | 576, 1167 |
| BlpI | GCTNAGC | 1 cut(s) | 1033 |
| BlsI | GCNGC | 2 cut(s) | 577, 1168 |
| Bme1390I | CCNGG | 4 cut(s) | 168, 324, 801, 1590 |
| Bme18I | GGWCC | 3 cut(s) | 170, 544, 1139 |
| BmeT110I | CYCGRG | 1 cut(s) | 69 |
| BmgBI | CACGTC | 1 cut(s) | 817 |
| BmgT120I | GGNCC | 4 cut(s) | 170, 290, 544, 1139 |
| BmiI | GGNNCC | 5 cut(s) | 421, 546, 757, 1141, 1411 |
| BmrFI | CCNGG | 4 cut(s) | 168, 324, 801, 1590 |
| BmsI | GCATC | 2 cut(s) | 898, 905 |
| BoxI | GACNNNNGTC | 2 cut(s) | 537, 1343 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1478, 1510 |
| BpmI | CTGGAG | 1 cut(s) | 1113 |
| Bpu10I | CCTNAGC | 1 cut(s) | 465 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1033 |
| BpuMI | CCSGG | 1 cut(s) | 324 |
| Bsa29I | ATCGAT | 1 cut(s) | 696 |
| BsaJI | CCNNGG | 3 cut(s) | 278, 799, 1199 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1398, 1428 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 249, 443, 739, 1136, 1553, 1699 |
| Bse118I | RCCGGY | 2 cut(s) | 597, 1243 |
| Bse1I | ACTGG | 3 cut(s) | 254, 401, 1319 |
| Bse3DI | GCAATG | 1 cut(s) | 717 |
| BseBI | CCWGG | 3 cut(s) | 168, 801, 1590 |
| BseCI | ATCGAT | 1 cut(s) | 696 |
| BseDI | CCNNGG | 3 cut(s) | 278, 799, 1199 |
| BseGI | GGATG | 3 cut(s) | 42, 1420, 1562 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 249, 443, 739, 1136, 1553, 1699 |
| BseMI | GCAATG | 1 cut(s) | 717 |
| BseMII | CTCAG | 3 cut(s) | 456, 1582, 1668 |
| BseNI | ACTGG | 3 cut(s) | 254, 401, 1319 |
| BseRI | GAGGAG | 1 cut(s) | 494 |
| BseSI | GKGCMC | 1 cut(s) | 1673 |
| BseXI | GCAGC | 2 cut(s) | 587, 1153 |
| Bsh1236I | CGCG | 1 cut(s) | 1757 |
| BshFI | GGCC | 2 cut(s) | 291, 597 |
| BshNI | GGYRCC | 1 cut(s) | 419 |
| BshVI | ATCGAT | 1 cut(s) | 696 |
| BsiHKAI | GWGCWC | 1 cut(s) | 187 |
| BsiHKCI | CYCGRG | 1 cut(s) | 69 |
| BsiSI | CCGG | 4 cut(s) | 323, 598, 1244, 1700 |
| BslFI | GGGAC | 4 cut(s) | 1046, 1125, 1351, 1505 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 249, 443, 739, 1136, 1553, 1699 |
| BsmAI | GTCTC | 2 cut(s) | 1615, 1673 |
| BsmFI | GGGAC | 4 cut(s) | 1046, 1125, 1351, 1505 |
| BsmI | GAATGC | 1 cut(s) | 1004 |
| BsnI | GGCC | 2 cut(s) | 291, 597 |
| BsoBI | CYCGRG | 1 cut(s) | 69 |
| Bsp1286I | GDGCHC | 2 cut(s) | 187, 1673 |
| Bsp1407I | TGTACA | 1 cut(s) | 1092 |
| Bsp143I | GATC | 4 cut(s) | 483, 497, 1315, 1516 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1033 |
| Bsp19I | CCATGG | 1 cut(s) | 278 |
| BspACI | CCGC | 3 cut(s) | 1107, 1274, 1757 |
| BspANI | GGCC | 2 cut(s) | 291, 597 |
| BspCNI | CTCAG | 3 cut(s) | 457, 1581, 1669 |
| BspDI | ATCGAT | 1 cut(s) | 696 |
| BspFNI | CGCG | 1 cut(s) | 1757 |
| BspLI | GGNNCC | 5 cut(s) | 421, 546, 757, 1141, 1411 |
| BspMI | ACCTGC | 1 cut(s) | 1440 |
| BspPI | GGATC | 3 cut(s) | 505, 1310, 1511 |
| BspQI | GCTCTTC | 1 cut(s) | 59 |
| BspT107I | GGYRCC | 1 cut(s) | 419 |
| BsrDI | GCAATG | 1 cut(s) | 717 |
| BsrFI | RCCGGY | 2 cut(s) | 597, 1243 |
| BsrGI | TGTACA | 1 cut(s) | 1092 |
| BsrI | ACTGG | 3 cut(s) | 254, 401, 1319 |
| BssAI | RCCGGY | 2 cut(s) | 597, 1243 |
| BssECI | CCNNGG | 3 cut(s) | 278, 799, 1199 |
| BssMI | GATC | 4 cut(s) | 483, 497, 1315, 1516 |
| BssT1I | CCWWGG | 1 cut(s) | 278 |
| Bst2UI | CCWGG | 3 cut(s) | 168, 801, 1590 |
| Bst4CI | ACNGT | 7 cut(s) | 12, 419, 739, 886, 1124, 1330, 1372 |
| Bst6I | CTCTTC | 3 cut(s) | 59, 471, 859 |
| BstAUI | TGTACA | 1 cut(s) | 1092 |
| BstC8I | GCNNGC | 8 cut(s) | 187, 384, 409, 438, 599, 1171, 1218, 1241 |
| BstDEI | CTNAG | 6 cut(s) | 465, 875, 1033, 1568, 1677, 1748 |
| BstDSI | CCRYGG | 1 cut(s) | 278 |
| BstF5I | GGATG | 3 cut(s) | 42, 1420, 1562 |
| BstFNI | CGCG | 1 cut(s) | 1757 |
| BstKTI | GATC | 4 cut(s) | 486, 500, 1318, 1519 |
| BstMAI | GTCTC | 2 cut(s) | 1615, 1673 |
| BstMBI | GATC | 4 cut(s) | 483, 497, 1315, 1516 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 182, 753 |
| BstNI | CCWGG | 3 cut(s) | 168, 801, 1590 |
| BstNSI | RCATGY | 2 cut(s) | 386, 1149 |
| BstPAI | GACNNNNGTC | 2 cut(s) | 537, 1343 |
| BstSCI | CCNGG | 4 cut(s) | 166, 322, 799, 1588 |
| BstSFI | CTRYAG | 3 cut(s) | 47, 882, 1015 |
| BstSLI | GKGCMC | 1 cut(s) | 1673 |
| BstUI | CGCG | 1 cut(s) | 1757 |
| BstV1I | GCAGC | 2 cut(s) | 587, 1153 |
| BstX2I | RGATCY | 2 cut(s) | 497, 1315 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1596 |
| BstYI | RGATCY | 2 cut(s) | 497, 1315 |
| Bsu15I | ATCGAT | 1 cut(s) | 696 |
| BsuI | GTATCC | 1 cut(s) | 190 |
| BsuRI | GGCC | 2 cut(s) | 291, 597 |
| BsuTUI | ATCGAT | 1 cut(s) | 696 |
| BtgI | CCRYGG | 1 cut(s) | 278 |
| BtgZI | GCGATG | 1 cut(s) | 1319 |
| BtrI | CACGTC | 1 cut(s) | 817 |
| BtsCI | GGATG | 3 cut(s) | 42, 1420, 1562 |
| BtsIMutI | CAGTG | 4 cut(s) | 247, 735, 1293, 1326 |
| BveI | ACCTGC | 1 cut(s) | 1440 |
| Cac8I | GCNNGC | 8 cut(s) | 187, 384, 409, 438, 599, 1171, 1218, 1241 |
| CaiI | CAGNNNCTG | 1 cut(s) | 899 |
| Cfr10I | RCCGGY | 2 cut(s) | 597, 1243 |
| Cfr13I | GGNCC | 4 cut(s) | 170, 290, 544, 1139 |
| ClaI | ATCGAT | 1 cut(s) | 696 |
| CseI | GACGC | 2 cut(s) | 1138, 1162 |
| Csp6I | GTAC | 2 cut(s) | 1093, 1125 |
| CviAII | CATG | 9 cut(s) | 142, 254, 279, 383, 618, 856, 1146, 1436, 1632 |
| CviQI | GTAC | 2 cut(s) | 1093, 1125 |
| DdeI | CTNAG | 6 cut(s) | 465, 875, 1033, 1568, 1677, 1748 |
| DpnI | GATC | 4 cut(s) | 485, 499, 1317, 1518 |
| DpnII | GATC | 4 cut(s) | 483, 497, 1315, 1516 |
| EaeI | YGGCCR | 1 cut(s) | 595 |
| Eam1104I | CTCTTC | 3 cut(s) | 59, 471, 859 |
| EarI | CTCTTC | 3 cut(s) | 59, 471, 859 |
| EciI | GGCGGA | 1 cut(s) | 1096 |
| Ecl136II | GAGCTC | 1 cut(s) | 185 |
| Eco130I | CCWWGG | 1 cut(s) | 278 |
| Eco24I | GRGCYC | 1 cut(s) | 187 |
| Eco32I | GATATC | 1 cut(s) | 1078 |
| Eco47I | GGWCC | 3 cut(s) | 170, 544, 1139 |
| Eco53kI | GAGCTC | 1 cut(s) | 185 |
| Eco57I | CTGAAG | 1 cut(s) | 933 |
| Eco88I | CYCGRG | 1 cut(s) | 69 |
| EcoICRI | GAGCTC | 1 cut(s) | 185 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1139 |
| EcoRII | CCWGG | 3 cut(s) | 166, 799, 1588 |
| EcoRV | GATATC | 1 cut(s) | 1078 |
| EcoT14I | CCWWGG | 1 cut(s) | 278 |
| EcoT38I | GRGCYC | 1 cut(s) | 187 |
| ErhI | CCWWGG | 1 cut(s) | 278 |
| FaeI | CATG | 9 cut(s) | 145, 257, 282, 386, 621, 859, 1149, 1439, 1635 |
| FaqI | GGGAC | 4 cut(s) | 1046, 1125, 1351, 1505 |
| FatI | CATG | 9 cut(s) | 141, 253, 278, 382, 617, 855, 1145, 1435, 1631 |
| FauNDI | CATATG | 1 cut(s) | 1614 |
| FblI | GTMKAC | 1 cut(s) | 5 |
| Fnu4HI | GCNGC | 2 cut(s) | 576, 1167 |
| FokI | GGATG | 3 cut(s) | 29, 1407, 1569 |
| FriOI | GRGCYC | 1 cut(s) | 187 |
| Fsp4HI | GCNGC | 2 cut(s) | 576, 1167 |
| FspBI | CTAG | 4 cut(s) | 120, 402, 1313, 1355 |
| GluI | GCNGC | 2 cut(s) | 576, 1167 |
| GsuI | CTGGAG | 1 cut(s) | 1113 |
| HaeIII | GGCC | 2 cut(s) | 291, 597 |
| HapII | CCGG | 4 cut(s) | 323, 598, 1244, 1700 |
| HgaI | GACGC | 2 cut(s) | 1138, 1162 |
| Hin1II | CATG | 9 cut(s) | 145, 257, 282, 386, 621, 859, 1149, 1439, 1635 |
| HincII | GTYRAC | 4 cut(s) | 6, 354, 532, 1326 |
| HindII | GTYRAC | 4 cut(s) | 6, 354, 532, 1326 |
| HindIII | AAGCTT | 1 cut(s) | 1030 |
| HinfI | GANTC | 9 cut(s) | 131, 976, 983, 1205, 1417, 1493, 1572, 1643, 1733 |
| HpaI | GTTAAC | 1 cut(s) | 1326 |
| HpaII | CCGG | 4 cut(s) | 323, 598, 1244, 1700 |
| HphI | GGTGA | 2 cut(s) | 232, 312 |
| Hpy188I | TCNGA | 8 cut(s) | 497, 678, 863, 901, 952, 1028, 1200, 1571 |
| Hpy188III | TCNNGA | 4 cut(s) | 464, 542, 980, 1130 |
| Hpy99I | CGWCG | 1 cut(s) | 645 |
| HpyAV | CCTTC | 3 cut(s) | 359, 1513, 1722 |
| HpyCH4III | ACNGT | 7 cut(s) | 12, 419, 739, 886, 1124, 1330, 1372 |
| HpyCH4IV | ACGT | 3 cut(s) | 635, 816, 1535 |
| HpyCH4V | TGCA | 7 cut(s) | 82, 551, 917, 1169, 1216, 1429, 1435 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 182, 753 |
| HpyF3I | CTNAG | 6 cut(s) | 465, 875, 1033, 1568, 1677, 1748 |
| HpySE526I | ACGT | 3 cut(s) | 635, 816, 1535 |
| Hsp92II | CATG | 9 cut(s) | 145, 257, 282, 386, 621, 859, 1149, 1439, 1635 |
| KroI | GCCGGC | 1 cut(s) | 597 |
| KroNI | GCCGGC | 1 cut(s) | 599 |
| KspAI | GTTAAC | 1 cut(s) | 1326 |
| Kzo9I | GATC | 4 cut(s) | 483, 497, 1315, 1516 |
| LguI | GCTCTTC | 1 cut(s) | 59 |
| LmnI | GCTCC | 5 cut(s) | 626, 761, 846, 961, 1695 |
| Lsp1109I | GCAGC | 2 cut(s) | 587, 1153 |
| LweI | GCATC | 2 cut(s) | 898, 905 |
| MaeI | CTAG | 4 cut(s) | 120, 402, 1313, 1355 |
| MaeII | ACGT | 3 cut(s) | 635, 816, 1535 |
| MaeIII | GTNAC | 4 cut(s) | 301, 392, 581, 1751 |
| MalI | GATC | 4 cut(s) | 485, 499, 1317, 1518 |
| MboI | GATC | 4 cut(s) | 483, 497, 1315, 1516 |
| MboII | GAAGA | 7 cut(s) | 46, 374, 488, 662, 665, 876, 1672 |
| MflI | RGATCY | 2 cut(s) | 497, 1315 |
| MhlI | GDGCHC | 2 cut(s) | 187, 1673 |
| MluCI | AATT | 9 cut(s) | 201, 331, 514, 562, 588, 832, 1474, 1507, 1760 |
| MlyI | GAGTC | 2 cut(s) | 1487, 1652 |
| MmeI | TCCRAC | 1 cut(s) | 841 |
| MnlI | CCTC | 9 cut(s) | 100, 185, 472, 743, 1194, 1400, 1482, 1530, 1707 |
| MroNI | GCCGGC | 1 cut(s) | 597 |
| MroXI | GAANNNNTTC | 2 cut(s) | 975, 1478 |
| MseI | TTAA | 6 cut(s) | 102, 210, 591, 1287, 1325, 1767 |
| MslI | CAYNNNNRTG | 3 cut(s) | 62, 252, 1434 |
| MspA1I | CMGCKG | 1 cut(s) | 578 |
| MspI | CCGG | 4 cut(s) | 323, 598, 1244, 1700 |
| MspR9I | CCNGG | 4 cut(s) | 168, 324, 801, 1590 |
| Mva1269I | GAATGC | 1 cut(s) | 1004 |
| MvaI | CCWGG | 3 cut(s) | 168, 801, 1590 |
| MvnI | CGCG | 1 cut(s) | 1757 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 182, 753 |
| NaeI | GCCGGC | 1 cut(s) | 599 |
| NciI | CCSGG | 1 cut(s) | 324 |
| NcoI | CCATGG | 1 cut(s) | 278 |
| NdeI | CATATG | 1 cut(s) | 1614 |
| NdeII | GATC | 4 cut(s) | 483, 497, 1315, 1516 |
| NgoMIV | GCCGGC | 1 cut(s) | 597 |
| NlaIII | CATG | 9 cut(s) | 145, 257, 282, 386, 621, 859, 1149, 1439, 1635 |
| NlaIV | GGNNCC | 5 cut(s) | 421, 546, 757, 1141, 1411 |
| NmeAIII | GCCGAG | 1 cut(s) | 1510 |
| NmuCI | GTSAC | 3 cut(s) | 301, 392, 1751 |
| NspI | RCATGY | 2 cut(s) | 386, 1149 |
| PaeI | GCATGC | 1 cut(s) | 386 |
| PaeR7I | CTCGAG | 1 cut(s) | 69 |
| PaqCI | CACCTGC | 1 cut(s) | 1440 |
| PciSI | GCTCTTC | 1 cut(s) | 59 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 693 |
| PctI | GAATGC | 1 cut(s) | 1004 |
| PdiI | GCCGGC | 1 cut(s) | 599 |
| PdmI | GAANNNNTTC | 2 cut(s) | 975, 1478 |
| PfeI | GAWTC | 7 cut(s) | 131, 976, 983, 1205, 1417, 1572, 1733 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 249, 1553 |
| PkrI | GCNGC | 2 cut(s) | 577, 1168 |
| PleI | GAGTC | 2 cut(s) | 1487, 1651 |
| PpsI | GAGTC | 2 cut(s) | 1487, 1651 |
| PpuMI | RGGWCCY | 1 cut(s) | 1139 |
| PshAI | GACNNNNGTC | 2 cut(s) | 537, 1343 |
| PshBI | ATTAAT | 2 cut(s) | 591, 1767 |
| Psp124BI | GAGCTC | 1 cut(s) | 187 |
| Psp1406I | AACGTT | 1 cut(s) | 1535 |
| Psp5II | RGGWCCY | 1 cut(s) | 1139 |
| Psp6I | CCWGG | 3 cut(s) | 166, 799, 1588 |
| PspGI | CCWGG | 3 cut(s) | 166, 799, 1588 |
| PspN4I | GGNNCC | 5 cut(s) | 421, 546, 757, 1141, 1411 |
| PspPI | GGNCC | 4 cut(s) | 170, 290, 544, 1139 |
| PspPPI | RGGWCCY | 1 cut(s) | 1139 |
| PspXI | VCTCGAGB | 1 cut(s) | 69 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 319, 351 |
| PstNI | CAGNNNCTG | 1 cut(s) | 899 |
| PsuI | RGATCY | 2 cut(s) | 497, 1315 |
| PvuII | CAGCTG | 1 cut(s) | 578 |
| RsaI | GTAC | 2 cut(s) | 1094, 1126 |
| RsaNI | GTAC | 2 cut(s) | 1093, 1125 |
| RseI | CAYNNNNRTG | 3 cut(s) | 62, 252, 1434 |
| SacI | GAGCTC | 1 cut(s) | 187 |
| SalI | GTCGAC | 1 cut(s) | 4 |
| SapI | GCTCTTC | 1 cut(s) | 59 |
| SaqAI | TTAA | 6 cut(s) | 102, 210, 591, 1287, 1325, 1767 |
| SatI | GCNGC | 2 cut(s) | 576, 1167 |
| Sau3AI | GATC | 4 cut(s) | 483, 497, 1315, 1516 |
| Sau96I | GGNCC | 4 cut(s) | 170, 290, 544, 1139 |
| SchI | GAGTC | 2 cut(s) | 1487, 1652 |
| ScrFI | CCNGG | 4 cut(s) | 168, 324, 801, 1590 |
| SduI | GDGCHC | 2 cut(s) | 187, 1673 |
| SfaNI | GCATC | 2 cut(s) | 898, 905 |
| SfcI | CTRYAG | 3 cut(s) | 47, 882, 1015 |
| Sfr274I | CTCGAG | 1 cut(s) | 69 |
| SinI | GGWCC | 3 cut(s) | 170, 544, 1139 |
| SlaI | CTCGAG | 1 cut(s) | 69 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 62, 252, 1434 |
| SmlI | CTYRAG | 1 cut(s) | 69 |
| SmoI | CTYRAG | 1 cut(s) | 69 |
| SphI | GCATGC | 1 cut(s) | 386 |
| Sse9I | AATT | 9 cut(s) | 201, 331, 514, 562, 588, 832, 1474, 1507, 1760 |
| SsiI | CCGC | 3 cut(s) | 1107, 1274, 1757 |
| SspI | AATATT | 1 cut(s) | 1183 |
| SspMI | CTAG | 4 cut(s) | 120, 402, 1313, 1355 |
| SstI | GAGCTC | 1 cut(s) | 187 |
| StyD4I | CCNGG | 4 cut(s) | 166, 322, 799, 1588 |
| StyI | CCWWGG | 1 cut(s) | 278 |
| TaaI | ACNGT | 7 cut(s) | 12, 419, 739, 886, 1124, 1330, 1372 |
| TaiI | ACGT | 3 cut(s) | 638, 819, 1538 |
| TaqI | TCGA | 4 cut(s) | 5, 70, 696, 1731 |
| TasI | AATT | 9 cut(s) | 201, 331, 514, 562, 588, 832, 1474, 1507, 1760 |
| TatI | WGTACW | 2 cut(s) | 1092, 1124 |
| TfiI | GAWTC | 7 cut(s) | 131, 976, 983, 1205, 1417, 1572, 1733 |
| Tru1I | TTAA | 6 cut(s) | 102, 210, 591, 1287, 1325, 1767 |
| Tru9I | TTAA | 6 cut(s) | 102, 210, 591, 1287, 1325, 1767 |
| TscAI | CASTG | 4 cut(s) | 254, 742, 1300, 1326 |
| TseFI | GTSAC | 3 cut(s) | 301, 392, 1751 |
| TseI | GCWGC | 2 cut(s) | 575, 1166 |
| Tsp45I | GTSAC | 3 cut(s) | 301, 392, 1751 |
| TspDTI | ATGAA | 6 cut(s) | 46, 489, 1266, 1284, 1380, 1528 |
| TspGWI | ACGGA | 1 cut(s) | 1402 |
| TspRI | CASTG | 4 cut(s) | 254, 742, 1300, 1326 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 249, 1553 |
| VpaK11BI | GGWCC | 3 cut(s) | 170, 544, 1139 |
| VspI | ATTAAT | 2 cut(s) | 591, 1767 |
| XapI | RAATTY | 3 cut(s) | 331, 514, 832 |
| XceI | RCATGY | 2 cut(s) | 386, 1149 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 276, 1554 |
| XhoI | CTCGAG | 1 cut(s) | 69 |
| XmiI | GTMKAC | 1 cut(s) | 5 |
| XmnI | GAANNNNTTC | 2 cut(s) | 975, 1478 |
| XspI | CTAG | 4 cut(s) | 120, 402, 1313, 1355 |