RchiOBHm_Chr6g0300491

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000320
Physical Location & Seq
Forward (+)
61321922 .. 61327962
6041 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ26986

Sequence Viewer

Length: 1776 bp
ATGTCGCCACGGATTCTAGTCTTTGCTGTTCTTATCCTGCCCTTTTTCTCTCTGGCTCTAGCCGAAGTGCATTACTATGATTTTGTAGTAAAGGAGGTAAACTTCAGCAGATTATGTGAAAATAAGCTCATGTTGGTTGTAAACGAAAGTTATCCAGGTCCAGCAATCCAAGCTCGCAAAGGGGATACAGTATACGTTAATGTTTACAATGAAGGGTTTTATGGCTTTACTATTCATTGGCATGGAGTAAAGCAGCCAAGAAACCCGTGGTTTGATGGCCCTGAGTATGTGACACAGTGTCCAATTGCACCCGGTACAAATTTCACTTATCAAGTGCAATTGACTAAGGAAGAAGGAACCGTATGGTGGCATGCTCATAGTGACTGGACAAGAGCCAGTGTTCACGGTGCTATTGTAGTTTTGCCTGCTCTTGGAACCACGTTTCCATTTCCTGAGCCGGATGAAGAGGAGATCATTGTTTTCGGATCTTGGTACCTGGGAAATTTGAGAGAACGAGTTGAGGAATCTTTGCAGCCTGGAAGCACCGAGACTCTACCCCAGTCAGATGCTTACACCATCAATGGCCAACCCGGCGATTTTCTTCCATGCTCCATAAATACAACATGGCGTCGTAGGGTGGACTACGGCAAGACTTATCTTCTTCGCCTGGTGAATGCTAACATCGATGCAGAGTTTTTCTTTGCAATTGCTGAGCACAACCTCACTGTGGTCGGACTGGACGGAGCCTACACAAAACCCATCAATACCAGATACATAGTGGTAACTCCTGGACAAACAATGGATGTATTGCTGAATGCAAATTCTACTCCGGGGCTCTATTACATGGCTGGTAGACAATACCTCAGTACTACATCCGACACCGGCTTCGACCATATGAATGCTACTGCAATCCTTCAATATAATGGCAACTATACCACAACCGACGCTCCTTTATTTCCCGAGCACCTTCCCATGTACATGTCCCAAGCAGAAGCGTTCAATTTCACCAATAAGATTAGAAGCTTAGCCACACCAGAATATCCCGTTAATGTCCCGAAGGAGAGTGAAGTTACTACACGAATGTTCATAACAGCTTCCATGAACTCTCTTTACTGTGCTCCAGATTTAGGCCCCACCTGCTTAAACATAAGCTTTGCTACAAGCGTCAATAACATCAGCTGGGTCAATCCAACTACAGACTCTATCTTGCAAGCCTACTACAAGAACATAAGCAATCCGATTTACGAAACAGATTTTCCAGACGAGCCGCCTACTTATTATAACTTCACAGATGGAGGGACGACGCTTTCTACAGTGTTGACAGTCCAAGCTACAAAGGTTAAGGTGCTAGAGTATAATGAAACTGTTGAGATTGTGTTCCAAGGGACTAATGTGATGGGAGGCGCCGTGAATCATCCAATGCATTTGCATGGACATAGTTTCTACGTGCTAGGATTTGGTTTCGGATCCTTTGACCCGTTGAAGGACCCTAAAGGCTATAATTTGGTCGATCCTCCTTATGTGGCTACATTCGTTACTCCAAAAAATGGATGGCTAACAATAAGATTTGTAGCTGATAATCCTGGCGTGTGGTTTTGGCATTGTCATGTAGAGAGACATATGACATGGGGTATGGAGGCCGCTTTTATAATCAAGGACGGAGGCACAGCTGAGACTAGTATGCTACCCCCACCAGCTCAAATGCCTTCCTGTGATGTTCCATTGGATTCTGCTATCAAAAATTACGAGGCCTTGATTGAGAAGCAAATTGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00053 ko01100 map00053 map01100
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

591

Amino Acids

66.35

Weight (kDa)

5.0

Isoelectric Point (pI)

42.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_3 PF07732 30 - 142 3e-38 Multicopper oxidase
Cu-oxidase PF00394 157 - 310 5e-33 Multicopper oxidase
Cu-oxidase_2 PF07731 418 - 553 6.6e-40 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000320)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g12640 FvH4_2g18980 FvH4_2g18990 FvH4_2g40270 FvH4_2g40270 FvH4_4g22740 FvH4_4g22740 FvH4_5g35760 FvH4_5g35760 FvH4_5g35760 FvH4_5g35760 FvH4_5g35760 FvH4_5g35760 FvH4_5g35760 FvH4_5g35760 FvH4_5g36460 FvH4_5g36460 FvH4_6g39950 FvH4_6g39970 FvH4_6g39983 FvH4_6g39990
rosa_chinensis RchiOBHm_Chr2g0154481 RchiOBHm_Chr2g0154511 RchiOBHm_Chr4g0428961 RchiOBHm_Chr6g0252431 RchiOBHm_Chr6g0273301 RchiOBHm_Chr6g0273631 RchiOBHm_Chr6g0273651 RchiOBHm_Chr6g0284751 RchiOBHm_Chr6g0284761 RchiOBHm_Chr6g0300491 RchiOBHm_Chr7g0237421 RchiOBHm_Chr7g0238581
rosa_laevigata RLG00000000906 RLG00000001006 RLG00000007110 RLG00000011333 RLG00000012721 RLG00000013605 RLG00000013626 RLG00000020780 RLG00000020782 RLG00000020783 RLG00000020786
rosa_multiflora Rmu_co8321753.1_g000001 Rmu_sc0000239.1_g000020 Rmu_sc0000686.1_g000001 Rmu_sc0000686.1_g000003 Rmu_sc0000686.1_g000005 Rmu_sc0001476.1_g000011 Rmu_sc0002231.1_g000002 Rmu_sc0002231.1_g000016 Rmu_sc0002690.1_g000003 Rmu_sc0002717.1_g000015 Rmu_sc0002923.1_g000026 Rmu_sc0003808.1_g000016 Rmu_sc0006475.1_g000008 Rmu_sc0006475.1_g000012 Rmu_sc0006475.1_g000018 Rmu_sc0014815.1_g000004 Rmu_sc0015313.1_g000012
rosa_roxburghii Rroxscaffold_2G00094270 Rroxscaffold_2G00094290 Rroxscaffold_3G00222950 Rroxscaffold_3G00224250 Rroxscaffold_5G00370630 Rroxscaffold_7G00167740 Rroxscaffold_7G00184410 Rroxscaffold_7G00195190 Rroxscaffold_7G00195460
rosa_rugosa Rorug02G0444600 Rorug04G0231100 Rorug04G0231200 Rorug06G0074000 Rorug06G0076800 Rorug06G0166400 Rorug06G0166500 Rorug06G0166600 Rorug06G0296400 Rorug07G0303000
rosa_samantha Rh2CG494600 Rh2CG494700 Rh2DG530800 Rh2DG530900 Rh2DG531000 Rh4AG287500 Rh4AG403800 Rh4BG293400 Rh4BG415100 Rh4DG290500 Rh6AG191000 Rh6AG191300 Rh6BG192500 Rh6BG194800 Rh6BG195200 Rh6BG279500 Rh6BG279600 Rh6DG183900 Rh6DG186600 Rh6DG274300 Rh6DG274500 Rh6DG409500 Rh7AG460600 Rh7AG468200 Rh7BG430200
rosa_wichuraiana Rw2G041820 Rw2G041830 Rw2G041840 Rw4G024930 Rw6G016470 Rw6G016720 Rw6G016730 Rw6G024020 Rw6G024030 Rw6G035790 Rw7G038130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1281, 1649
AarI CACCTGC 1 cut(s) 1145
Acc36I ACCTGC 1 cut(s) 1145
Acc65I GGTACC 1 cut(s) 492
AccB1I GGYRCC 2 cut(s) 492, 1403
AccB7I CCANNNNNTGG 1 cut(s) 1547
AccI GTMKAC 2 cut(s) 192, 853
AciI CCGC 2 cut(s) 1268, 1641
AclWI GGATC 4 cut(s) 493, 1461, 1474, 1505
AcoI YGGCCR 1 cut(s) 583
AcsI RAATTY 3 cut(s) 319, 502, 820
AcuI CTGAAG 1 cut(s) 88
AcyI GRCGYC 2 cut(s) 628, 1404
AfaI GTAC 4 cut(s) 316, 494, 868, 977
AfiI CCNNNNNNNGG 6 cut(s) 366, 431, 727, 1127, 1483, 1547
AflIII ACRYGT 1 cut(s) 978
AgsI TTSAA 3 cut(s) 917, 1000, 1483
AhdI GACNNNNNGTC 1 cut(s) 297
AhlI ACTAGT 1 cut(s) 1676
AjnI CCWGG 6 cut(s) 154, 495, 535, 666, 787, 1582
AloI GAACNNNNNNTCC 2 cut(s) 427, 459
Alw21I GWGCWC 3 cut(s) 717, 966, 1120
Alw26I GTCTC 3 cut(s) 542, 1609, 1667
AlwI GGATC 4 cut(s) 493, 1461, 1474, 1505
Ama87I CYCGRG 1 cut(s) 959
AoxI GGCC 5 cut(s) 277, 583, 1129, 1638, 1749
ApeKI GCWGC 2 cut(s) 253, 532
ApoI RAATTY 3 cut(s) 319, 502, 820
ArsI GACNNNNNNTTYG 2 cut(s) 869, 901
Asp718I GGTACC 1 cut(s) 492
AspLEI GCGC 1 cut(s) 1406
AspS9I GGNCC 4 cut(s) 158, 278, 1130, 1486
AsuC2I CCSGG 3 cut(s) 312, 591, 831
AsuHPI GGTGA 2 cut(s) 682, 997
AvaI CYCGRG 1 cut(s) 959
AvaII GGWCC 2 cut(s) 158, 1486
BalI TGGCCA 1 cut(s) 585
BamHI GGATCC 1 cut(s) 1466
BanI GGYRCC 2 cut(s) 492, 1403
BanII GRGCYC 1 cut(s) 837
Bbv12I GWGCWC 3 cut(s) 717, 966, 1120
BbvI GCAGC 2 cut(s) 265, 544
BccI CCATC 6 cut(s) 269, 584, 767, 1286, 1390, 1545
BceAI ACGGC 2 cut(s) 661, 1391
BciT130I CCWGG 6 cut(s) 156, 497, 537, 668, 789, 1584
BciVI GTATCC 1 cut(s) 178
BcnI CCSGG 3 cut(s) 312, 591, 831
BcoDI GTCTC 3 cut(s) 542, 1609, 1667
BcuI ACTAGT 1 cut(s) 1676
BfaI CTAG 5 cut(s) 17, 59, 1349, 1451, 1677
BfmI CTRYAG 2 cut(s) 1194, 1311
BfoI RGCGCY 1 cut(s) 1407
BfuAI ACCTGC 1 cut(s) 1145
BfuI GTATCC 1 cut(s) 178
BglI GCCNNNNNGGC 1 cut(s) 591
BisI GCNGC 4 cut(s) 254, 533, 1268, 1641
BlpI GCTNAGC 2 cut(s) 711, 1024
BlsI GCNGC 4 cut(s) 255, 534, 1269, 1642
BmcAI AGTACT 1 cut(s) 868
Bme1390I CCNGG 9 cut(s) 156, 312, 497, 537, 591, 668, 789, 831, 1584
Bme18I GGWCC 2 cut(s) 158, 1486
BmeRI GACNNNNNGTC 1 cut(s) 297
BmeT110I CYCGRG 1 cut(s) 959
BmgT120I GGNCC 4 cut(s) 158, 278, 1130, 1486
BmiI GGNNCC 8 cut(s) 358, 436, 494, 745, 1132, 1405, 1468, 1488
BmrFI CCNGG 9 cut(s) 156, 312, 497, 537, 591, 668, 789, 831, 1584
BmrI ACTGGG 1 cut(s) 553
BmsI GCATC 2 cut(s) 556, 676
BmuI ACTGGG 1 cut(s) 553
BpmI CTGGAG 1 cut(s) 1104
Bpu10I CCTNAGC 1 cut(s) 453
Bpu1102I GCTNAGC 2 cut(s) 711, 1024
BpuMI CCSGG 3 cut(s) 312, 591, 831
Bsa29I ATCGAT 1 cut(s) 684
BsaAI YACGTR 1 cut(s) 1447
BsaHI GRCGYC 2 cut(s) 628, 1404
BsaJI CCNNGG 5 cut(s) 8, 266, 496, 830, 1381
BsaXI ACNNNNNCTCC 4 cut(s) 931, 961, 1392, 1422
Bsc4I CCNNNNNNNGG 6 cut(s) 366, 431, 727, 1127, 1483, 1547
Bse118I RCCGGY 1 cut(s) 881
Bse1I ACTGG 4 cut(s) 389, 396, 559, 741
BseBI CCWGG 6 cut(s) 156, 497, 537, 668, 789, 1584
BseCI ATCGAT 1 cut(s) 684
BseDI CCNNGG 5 cut(s) 8, 266, 496, 830, 1381
BseGI GGATG 5 cut(s) 466, 808, 872, 1414, 1556
BseLI CCNNNNNNNGG 6 cut(s) 366, 431, 727, 1127, 1483, 1547
BseMII CTCAG 5 cut(s) 273, 444, 702, 877, 1662
BseNI ACTGG 4 cut(s) 389, 396, 559, 741
BseRI GAGGAG 1 cut(s) 482
BseXI GCAGC 2 cut(s) 265, 544
BseYI CCCAGC 1 cut(s) 1179
BshFI GGCC 5 cut(s) 279, 585, 1131, 1640, 1751
BshNI GGYRCC 2 cut(s) 492, 1403
BshVI ATCGAT 1 cut(s) 684
BsiHKAI GWGCWC 3 cut(s) 717, 966, 1120
BsiHKCI CYCGRG 1 cut(s) 959
BsiSI CCGG 5 cut(s) 312, 458, 591, 830, 882
BslFI GGGAC 4 cut(s) 967, 1037, 1312, 1399
BslI CCNNNNNNNGG 6 cut(s) 366, 431, 727, 1127, 1483, 1547
BsmAI GTCTC 3 cut(s) 542, 1609, 1667
BsmFI GGGAC 4 cut(s) 967, 1037, 1312, 1399
BsmI GAATGC 3 cut(s) 679, 820, 904
BsnI GGCC 5 cut(s) 279, 585, 1131, 1640, 1751
BsoBI CYCGRG 1 cut(s) 959
Bsp1286I GDGCHC 4 cut(s) 717, 837, 966, 1120
Bsp1407I TGTACA 1 cut(s) 975
Bsp143I GATC 4 cut(s) 471, 485, 1466, 1510
Bsp1720I GCTNAGC 2 cut(s) 711, 1024
BspACI CCGC 2 cut(s) 1268, 1641
BspANI GGCC 5 cut(s) 279, 585, 1131, 1640, 1751
BspCNI CTCAG 5 cut(s) 274, 445, 703, 876, 1663
BspDI ATCGAT 1 cut(s) 684
BspLI GGNNCC 8 cut(s) 358, 436, 494, 745, 1132, 1405, 1468, 1488
BspMI ACCTGC 1 cut(s) 1145
BspPI GGATC 4 cut(s) 493, 1461, 1474, 1505
BspT107I GGYRCC 2 cut(s) 492, 1403
BsrFI RCCGGY 1 cut(s) 881
BsrGI TGTACA 1 cut(s) 975
BsrI ACTGG 4 cut(s) 389, 396, 559, 741
BssAI RCCGGY 1 cut(s) 881
BssECI CCNNGG 5 cut(s) 8, 266, 496, 830, 1381
BssMI GATC 4 cut(s) 471, 485, 1466, 1510
BssNAI GTATAC 1 cut(s) 193
BssNI GRCGYC 2 cut(s) 628, 1404
BssT1I CCWWGG 1 cut(s) 1381
Bst1107I GTATAC 1 cut(s) 193
Bst2UI CCWGG 6 cut(s) 156, 497, 537, 668, 789, 1584
Bst4CI ACNGT 9 cut(s) 190, 297, 361, 407, 727, 1115, 1315, 1324, 1366
Bst6I CTCTTC 1 cut(s) 459
BstACI GRCGYC 2 cut(s) 628, 1404
BstAUI TGTACA 1 cut(s) 975
BstBAI YACGTR 1 cut(s) 1447
BstC8I GCNNGC 4 cut(s) 175, 372, 426, 1212
BstDEI CTNAG 7 cut(s) 282, 345, 453, 711, 863, 1024, 1671
BstDSI CCRYGG 2 cut(s) 8, 266
BstF5I GGATG 5 cut(s) 466, 808, 872, 1414, 1556
BstH2I RGCGCY 1 cut(s) 1407
BstHHI GCGC 1 cut(s) 1406
BstKTI GATC 4 cut(s) 474, 488, 1469, 1513
BstMAI GTCTC 3 cut(s) 542, 1609, 1667
BstMBI GATC 4 cut(s) 471, 485, 1466, 1510
BstMWI GCNNNNNNNGC 3 cut(s) 170, 591, 1137
BstNI CCWGG 6 cut(s) 156, 497, 537, 668, 789, 1584
BstNSI RCATGY 2 cut(s) 374, 982
BstSCI CCNGG 9 cut(s) 154, 310, 495, 535, 589, 666, 787, 829, 1582
BstSFI CTRYAG 2 cut(s) 1194, 1311
BstV1I GCAGC 2 cut(s) 265, 544
BstX2I RGATCY 2 cut(s) 485, 1466
BstYI RGATCY 2 cut(s) 485, 1466
BstZ17I GTATAC 1 cut(s) 193
Bsu15I ATCGAT 1 cut(s) 684
BsuI GTATCC 1 cut(s) 178
BsuRI GGCC 5 cut(s) 279, 585, 1131, 1640, 1751
BsuTUI ATCGAT 1 cut(s) 684
BtgI CCRYGG 2 cut(s) 8, 266
BtsCI GGATG 5 cut(s) 466, 808, 872, 1414, 1556
BtsIMutI CAGTG 4 cut(s) 302, 403, 723, 1320
BveI ACCTGC 1 cut(s) 1145
Cac8I GCNNGC 4 cut(s) 175, 372, 426, 1212
CfoI GCGC 1 cut(s) 1406
Cfr10I RCCGGY 1 cut(s) 881
Cfr13I GGNCC 4 cut(s) 158, 278, 1130, 1486
ClaI ATCGAT 1 cut(s) 684
CseI GACGC 4 cut(s) 617, 953, 1153, 1312
Csp6I GTAC 4 cut(s) 315, 493, 867, 976
CviQI GTAC 4 cut(s) 315, 493, 867, 976
DdeI CTNAG 7 cut(s) 282, 345, 453, 711, 863, 1024, 1671
DinI GGCGCC 1 cut(s) 1405
DpnI GATC 4 cut(s) 473, 487, 1468, 1512
DpnII GATC 4 cut(s) 471, 485, 1466, 1510
DriI GACNNNNNGTC 1 cut(s) 297
EaeI YGGCCR 1 cut(s) 583
Eam1104I CTCTTC 1 cut(s) 459
Eam1105I GACNNNNNGTC 1 cut(s) 297
EarI CTCTTC 1 cut(s) 459
Eco130I CCWWGG 1 cut(s) 1381
Eco147I AGGCCT 1 cut(s) 1751
Eco24I GRGCYC 1 cut(s) 837
Eco47I GGWCC 2 cut(s) 158, 1486
Eco57I CTGAAG 1 cut(s) 88
Eco88I CYCGRG 1 cut(s) 959
EcoO109I RGGNCCY 2 cut(s) 1130, 1486
EcoRII CCWGG 6 cut(s) 154, 495, 535, 666, 787, 1582
EcoT14I CCWWGG 1 cut(s) 1381
EcoT22I ATGCAT 1 cut(s) 1425
EcoT38I GRGCYC 1 cut(s) 837
EgeI GGCGCC 1 cut(s) 1405
EheI GGCGCC 1 cut(s) 1405
ErhI CCWWGG 1 cut(s) 1381
FaqI GGGAC 4 cut(s) 967, 1037, 1312, 1399
FauNDI CATATG 2 cut(s) 894, 1620
FblI GTMKAC 2 cut(s) 192, 853
Fnu4HI GCNGC 4 cut(s) 254, 533, 1268, 1641
FokI GGATG 5 cut(s) 473, 815, 859, 1401, 1563
FriOI GRGCYC 1 cut(s) 837
Fsp4HI GCNGC 4 cut(s) 254, 533, 1268, 1641
FspBI CTAG 5 cut(s) 17, 59, 1349, 1451, 1677
GlaI GCGC 1 cut(s) 1405
GluI GCNGC 4 cut(s) 254, 533, 1268, 1641
GsaI CCCAGC 1 cut(s) 1183
GsuI CTGGAG 1 cut(s) 1104
HaeII RGCGCY 1 cut(s) 1407
HaeIII GGCC 5 cut(s) 279, 585, 1131, 1640, 1751
HapII CCGG 5 cut(s) 312, 458, 591, 830, 882
HgaI GACGC 4 cut(s) 617, 953, 1153, 1312
HhaI GCGC 1 cut(s) 1406
Hin1I GRCGYC 2 cut(s) 628, 1404
Hin6I GCGC 1 cut(s) 1404
HinP1I GCGC 1 cut(s) 1404
HincII GTYRAC 1 cut(s) 1320
HindII GTYRAC 1 cut(s) 1320
HindIII AAGCTT 2 cut(s) 1021, 1150
HinfI GANTC 6 cut(s) 13, 524, 550, 1199, 1411, 1727
HpaII CCGG 5 cut(s) 312, 458, 591, 830, 882
HphI GGTGA 2 cut(s) 682, 997
Hpy166II GTNNAC 8 cut(s) 100, 142, 193, 205, 403, 640, 854, 1320
Hpy188I TCNGA 6 cut(s) 485, 565, 734, 877, 1239, 1466
Hpy188III TCNNGA 5 cut(s) 452, 959, 1054, 1121, 1259
Hpy8I GTNNAC 8 cut(s) 100, 142, 193, 205, 403, 640, 854, 1320
Hpy99I CGWCG 3 cut(s) 633, 947, 1306
HpyAV CCTTC 7 cut(s) 206, 347, 923, 977, 1051, 1477, 1716
HpyCH4III ACNGT 9 cut(s) 190, 297, 361, 407, 727, 1115, 1315, 1324, 1366
HpyCH4IV ACGT 3 cut(s) 195, 440, 1446
HpyF10VI GCNNNNNNNGC 3 cut(s) 170, 591, 1137
HpyF3I CTNAG 7 cut(s) 282, 345, 453, 711, 863, 1024, 1671
HpySE526I ACGT 3 cut(s) 195, 440, 1446
Hsp92I GRCGYC 2 cut(s) 628, 1404
HspAI GCGC 1 cut(s) 1404
KasI GGCGCC 1 cut(s) 1403
KpnI GGTACC 1 cut(s) 496
Kzo9I GATC 4 cut(s) 471, 485, 1466, 1510
LmnI GCTCC 4 cut(s) 614, 743, 952, 1123
Lsp1109I GCAGC 2 cut(s) 265, 544
LweI GCATC 2 cut(s) 556, 676
MaeI CTAG 5 cut(s) 17, 59, 1349, 1451, 1677
MaeII ACGT 3 cut(s) 195, 440, 1446
MaeIII GTNAC 5 cut(s) 289, 380, 781, 1069, 1534
MalI GATC 4 cut(s) 473, 487, 1468, 1512
MboI GATC 4 cut(s) 471, 485, 1466, 1510
MboII GAAGA 5 cut(s) 362, 476, 593, 650, 653
MfeI CAATTG 3 cut(s) 303, 338, 705
MflI RGATCY 2 cut(s) 485, 1466
MhlI GDGCHC 4 cut(s) 717, 837, 966, 1120
MlsI TGGCCA 1 cut(s) 585
MluNI TGGCCA 1 cut(s) 585
Mly113I GGCGCC 1 cut(s) 1404
MlyI GAGTC 2 cut(s) 544, 1193
MmeI TCCRAC 3 cut(s) 712, 900, 1214
Mox20I TGGCCA 1 cut(s) 585
Mph1103I ATGCAT 1 cut(s) 1425
MscI TGGCCA 1 cut(s) 585
MseI TTAA 4 cut(s) 198, 1047, 1142, 1341
MslI CAYNNNNRTG 6 cut(s) 75, 240, 897, 977, 1428, 1605
Msp20I TGGCCA 1 cut(s) 585
MspA1I CMGCKG 2 cut(s) 1179, 1670
MspI CCGG 5 cut(s) 312, 458, 591, 830, 882
MspR9I CCNGG 9 cut(s) 156, 312, 497, 537, 591, 668, 789, 831, 1584
MunI CAATTG 3 cut(s) 303, 338, 705
Mva1269I GAATGC 3 cut(s) 679, 820, 904
MvaI CCWGG 6 cut(s) 156, 497, 537, 668, 789, 1584
MwoI GCNNNNNNNGC 3 cut(s) 170, 591, 1137
NarI GGCGCC 1 cut(s) 1404
NciI CCSGG 3 cut(s) 312, 591, 831
NdeI CATATG 2 cut(s) 894, 1620
NdeII GATC 4 cut(s) 471, 485, 1466, 1510
NlaIV GGNNCC 8 cut(s) 358, 436, 494, 745, 1132, 1405, 1468, 1488
NmuCI GTSAC 2 cut(s) 289, 380
NsiI ATGCAT 1 cut(s) 1425
NspI RCATGY 2 cut(s) 374, 982
PaeI GCATGC 1 cut(s) 374
PaqCI CACCTGC 1 cut(s) 1145
PceI AGGCCT 1 cut(s) 1751
PciI ACATGT 1 cut(s) 978
PctI GAATGC 3 cut(s) 679, 820, 904
PfeI GAWTC 4 cut(s) 13, 524, 1411, 1727
PflMI CCANNNNNTGG 1 cut(s) 1547
PfoI TCCNGGA 1 cut(s) 787
PkrI GCNGC 4 cut(s) 255, 534, 1269, 1642
PleI GAGTC 2 cut(s) 544, 1193
PluTI GGCGCC 1 cut(s) 1407
PpsI GAGTC 2 cut(s) 544, 1193
Ppu21I YACGTR 1 cut(s) 1447
PpuMI RGGWCCY 1 cut(s) 1486
PscI ACATGT 1 cut(s) 978
PsiI TTATAA 2 cut(s) 1281, 1649
Psp5II RGGWCCY 1 cut(s) 1486
Psp6I CCWGG 6 cut(s) 154, 495, 535, 666, 787, 1582
PspFI CCCAGC 1 cut(s) 1179
PspGI CCWGG 6 cut(s) 154, 495, 535, 666, 787, 1582
PspN4I GGNNCC 8 cut(s) 358, 436, 494, 745, 1132, 1405, 1468, 1488
PspPI GGNCC 4 cut(s) 158, 278, 1130, 1486
PspPPI RGGWCCY 1 cut(s) 1486
PsrI GAACNNNNNNTAC 2 cut(s) 1094, 1126
PsuI RGATCY 2 cut(s) 485, 1466
PvuII CAGCTG 2 cut(s) 1179, 1670
RsaI GTAC 4 cut(s) 316, 494, 868, 977
RsaNI GTAC 4 cut(s) 315, 493, 867, 976
RseI CAYNNNNRTG 6 cut(s) 75, 240, 897, 977, 1428, 1605
SaqAI TTAA 4 cut(s) 198, 1047, 1142, 1341
SatI GCNGC 4 cut(s) 254, 533, 1268, 1641
Sau3AI GATC 4 cut(s) 471, 485, 1466, 1510
Sau96I GGNCC 4 cut(s) 158, 278, 1130, 1486
ScaI AGTACT 1 cut(s) 868
SchI GAGTC 2 cut(s) 544, 1193
ScrFI CCNGG 9 cut(s) 156, 312, 497, 537, 591, 668, 789, 831, 1584
SduI GDGCHC 4 cut(s) 717, 837, 966, 1120
SfaNI GCATC 2 cut(s) 556, 676
SfcI CTRYAG 2 cut(s) 1194, 1311
SfoI GGCGCC 1 cut(s) 1405
SinI GGWCC 2 cut(s) 158, 1486
SmiMI CAYNNNNRTG 6 cut(s) 75, 240, 897, 977, 1428, 1605
SpeI ACTAGT 1 cut(s) 1676
SphI GCATGC 1 cut(s) 374
SseBI AGGCCT 1 cut(s) 1751
SsiI CCGC 2 cut(s) 1268, 1641
SspDI GGCGCC 1 cut(s) 1403
SspMI CTAG 5 cut(s) 17, 59, 1349, 1451, 1677
StuI AGGCCT 1 cut(s) 1751
StyD4I CCNGG 9 cut(s) 154, 310, 495, 535, 589, 666, 787, 829, 1582
StyI CCWWGG 1 cut(s) 1381
TaaI ACNGT 9 cut(s) 190, 297, 361, 407, 727, 1115, 1315, 1324, 1366
TaiI ACGT 3 cut(s) 198, 443, 1449
TaqI TCGA 3 cut(s) 684, 888, 1509
TatI WGTACW 2 cut(s) 866, 975
TauI GCSGC 2 cut(s) 1270, 1643
TfiI GAWTC 4 cut(s) 13, 524, 1411, 1727
Tru1I TTAA 4 cut(s) 198, 1047, 1142, 1341
Tru9I TTAA 4 cut(s) 198, 1047, 1142, 1341
TscAI CASTG 4 cut(s) 302, 403, 730, 1320
TseFI GTSAC 2 cut(s) 289, 380
TseI GCWGC 2 cut(s) 253, 532
Tsp45I GTSAC 2 cut(s) 289, 380
TspDTI ATGAA 7 cut(s) 224, 225, 477, 911, 1075, 1115, 1374
TspGWI ACGGA 3 cut(s) 25, 756, 1674
TspRI CASTG 4 cut(s) 302, 403, 730, 1320
Van91I CCANNNNNTGG 1 cut(s) 1547
VpaK11BI GGWCC 2 cut(s) 158, 1486
XapI RAATTY 3 cut(s) 319, 502, 820
XceI RCATGY 2 cut(s) 374, 982
XcmI CCANNNNNNNNNTGG 3 cut(s) 264, 775, 1548
XmiI GTMKAC 2 cut(s) 192, 853
XspI CTAG 5 cut(s) 17, 59, 1349, 1451, 1677
ZrmI AGTACT 1 cut(s) 868
Zsp2I ATGCAT 1 cut(s) 1425
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.