Lignin degradation and detoxification of lignin-derived products
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 4 cut(s) | 506, 1106, 1287, 1362 |
| Acc36I | ACCTGC | 1 cut(s) | 582 |
| AccI | GTMKAC | 1 cut(s) | 1693 |
| AciI | CCGC | 3 cut(s) | 67, 930, 1274 |
| AclWI | GGATC | 5 cut(s) | 887, 900, 1468, 1487, 1511 |
| AcoI | YGGCCR | 1 cut(s) | 853 |
| AcsI | RAATTY | 3 cut(s) | 39, 346, 1025 |
| AcuI | CTGAAG | 1 cut(s) | 396 |
| AdeI | CACNNNGTG | 1 cut(s) | 324 |
| AfaI | GTAC | 4 cut(s) | 314, 521, 1001, 1672 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 458, 1230, 1553 |
| AgsI | TTSAA | 6 cut(s) | 86, 147, 941, 1173, 1357, 1376 |
| AhlI | ACTAGT | 1 cut(s) | 541 |
| AjnI | CCWGG | 7 cut(s) | 181, 337, 515, 610, 850, 1141, 1588 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 454, 486 |
| AluBI | AGCT | 5 cut(s) | 869, 1047, 1191, 1446, 1703 |
| AluI | AGCT | 5 cut(s) | 869, 1047, 1191, 1446, 1703 |
| Alw26I | GTCTC | 3 cut(s) | 491, 609, 1673 |
| AlwI | GGATC | 5 cut(s) | 887, 900, 1468, 1487, 1511 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 731 |
| AoxI | GGCC | 2 cut(s) | 754, 853 |
| ApeKI | GCWGC | 3 cut(s) | 869, 1013, 1016 |
| ApoI | RAATTY | 3 cut(s) | 39, 346, 1025 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 128 |
| AspS9I | GGNCC | 2 cut(s) | 185, 305 |
| AsuC2I | CCSGG | 1 cut(s) | 1036 |
| AsuHPI | GGTGA | 5 cut(s) | 64, 124, 736, 1021, 1357 |
| AvaII | GGWCC | 2 cut(s) | 185, 305 |
| BalI | TGGCCA | 1 cut(s) | 855 |
| BamHI | GGATCC | 1 cut(s) | 892 |
| BanII | GRGCYC | 1 cut(s) | 1412 |
| BbvI | GCAGC | 3 cut(s) | 856, 1025, 1028 |
| BccI | CCATC | 3 cut(s) | 296, 557, 814 |
| BceAI | ACGGC | 1 cut(s) | 682 |
| BciT130I | CCWGG | 7 cut(s) | 183, 339, 517, 612, 852, 1143, 1590 |
| BcnI | CCSGG | 1 cut(s) | 1036 |
| BcoDI | GTCTC | 3 cut(s) | 491, 609, 1673 |
| BcuI | ACTAGT | 1 cut(s) | 541 |
| BfaI | CTAG | 4 cut(s) | 80, 542, 758, 873 |
| BfmI | CTRYAG | 2 cut(s) | 1068, 1228 |
| BfuAI | ACCTGC | 1 cut(s) | 582 |
| BisI | GCNGC | 3 cut(s) | 870, 1014, 1017 |
| BlsI | GCNGC | 3 cut(s) | 871, 1015, 1018 |
| Bme1390I | CCNGG | 8 cut(s) | 183, 339, 517, 612, 852, 1036, 1143, 1590 |
| Bme18I | GGWCC | 2 cut(s) | 185, 305 |
| BmgT120I | GGNCC | 2 cut(s) | 185, 305 |
| BmiI | GGNNCC | 3 cut(s) | 463, 766, 894 |
| BmrFI | CCNGG | 8 cut(s) | 183, 339, 517, 612, 852, 1036, 1143, 1590 |
| BmsI | GCATC | 4 cut(s) | 165, 521, 577, 977 |
| BoxI | GACNNNNGTC | 1 cut(s) | 621 |
| BpmI | CTGGAG | 1 cut(s) | 616 |
| BpuEI | CTTGAG | 2 cut(s) | 868, 1648 |
| BpuMI | CCSGG | 1 cut(s) | 1036 |
| BsaAI | YACGTR | 1 cut(s) | 1453 |
| BsaBI | GATNNNNATC | 1 cut(s) | 443 |
| BsaI | GGTCTC | 1 cut(s) | 491 |
| BsaJI | CCNNGG | 6 cut(s) | 238, 293, 1333, 1387, 1588, 1589 |
| BsaWI | WCCGGW | 1 cut(s) | 484 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 201, 231 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 458, 1230, 1553 |
| Bse1I | ACTGG | 3 cut(s) | 269, 633, 910 |
| Bse8I | GATNNNNATC | 1 cut(s) | 443 |
| BseBI | CCWGG | 7 cut(s) | 183, 339, 517, 612, 852, 1143, 1590 |
| BseDI | CCNNGG | 6 cut(s) | 238, 293, 1333, 1387, 1588, 1589 |
| BseGI | GGATG | 7 cut(s) | 493, 512, 565, 829, 1138, 1420, 1495 |
| BseJI | GATNNNNATC | 1 cut(s) | 443 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 458, 1230, 1553 |
| BseMII | CTCAG | 1 cut(s) | 1668 |
| BseNI | ACTGG | 3 cut(s) | 269, 633, 910 |
| BseRI | GAGGAG | 1 cut(s) | 821 |
| BseXI | GCAGC | 3 cut(s) | 856, 1025, 1028 |
| BseYI | CCCAGC | 1 cut(s) | 1699 |
| BshFI | GGCC | 2 cut(s) | 756, 855 |
| BsiSI | CCGG | 2 cut(s) | 485, 1036 |
| BslFI | GGGAC | 3 cut(s) | 1061, 1351, 1405 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 458, 1230, 1553 |
| BsmAI | GTCTC | 3 cut(s) | 491, 609, 1673 |
| BsmFI | GGGAC | 3 cut(s) | 1061, 1351, 1405 |
| BsnI | GGCC | 2 cut(s) | 756, 855 |
| Bso31I | GGTCTC | 1 cut(s) | 491 |
| Bsp1286I | GDGCHC | 1 cut(s) | 1412 |
| Bsp143I | GATC | 5 cut(s) | 892, 1123, 1460, 1492, 1516 |
| BspACI | CCGC | 3 cut(s) | 67, 930, 1274 |
| BspANI | GGCC | 2 cut(s) | 756, 855 |
| BspCNI | CTCAG | 1 cut(s) | 1669 |
| BspLI | GGNNCC | 3 cut(s) | 463, 766, 894 |
| BspMI | ACCTGC | 1 cut(s) | 582 |
| BspPI | GGATC | 5 cut(s) | 887, 900, 1468, 1487, 1511 |
| BspTNI | GGTCTC | 1 cut(s) | 491 |
| BsrI | ACTGG | 3 cut(s) | 269, 633, 910 |
| BssECI | CCNNGG | 6 cut(s) | 238, 293, 1333, 1387, 1588, 1589 |
| BssMI | GATC | 5 cut(s) | 892, 1123, 1460, 1492, 1516 |
| BssT1I | CCWWGG | 3 cut(s) | 238, 1333, 1387 |
| Bst2UI | CCWGG | 7 cut(s) | 183, 339, 517, 612, 852, 1143, 1590 |
| Bst4CI | ACNGT | 7 cut(s) | 217, 427, 748, 1111, 1330, 1610, 1751 |
| Bst6I | CTCTTC | 2 cut(s) | 996, 1174 |
| BstBAI | YACGTR | 1 cut(s) | 1453 |
| BstC8I | GCNNGC | 1 cut(s) | 399 |
| BstDEI | CTNAG | 2 cut(s) | 1217, 1677 |
| BstDSI | CCRYGG | 1 cut(s) | 293 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1228 |
| BstF5I | GGATG | 7 cut(s) | 493, 512, 565, 829, 1138, 1420, 1495 |
| BstKTI | GATC | 5 cut(s) | 895, 1126, 1463, 1495, 1519 |
| BstMAI | GTCTC | 3 cut(s) | 491, 609, 1673 |
| BstMBI | GATC | 5 cut(s) | 892, 1123, 1460, 1492, 1516 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 815 |
| BstNI | CCWGG | 7 cut(s) | 183, 339, 517, 612, 852, 1143, 1590 |
| BstNSI | RCATGY | 2 cut(s) | 401, 1711 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 621 |
| BstSCI | CCNGG | 8 cut(s) | 181, 337, 515, 610, 850, 1034, 1141, 1588 |
| BstSFI | CTRYAG | 2 cut(s) | 1068, 1228 |
| BstV1I | GCAGC | 3 cut(s) | 856, 1025, 1028 |
| BstX2I | RGATCY | 2 cut(s) | 892, 1460 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 851, 1003 |
| BstYI | RGATCY | 2 cut(s) | 892, 1460 |
| BsuRI | GGCC | 2 cut(s) | 756, 855 |
| BtgI | CCRYGG | 1 cut(s) | 293 |
| BtgZI | GCGATG | 1 cut(s) | 833 |
| BtsCI | GGATG | 7 cut(s) | 493, 512, 565, 829, 1138, 1420, 1495 |
| BtsI | GCAGTG | 2 cut(s) | 329, 1644 |
| BtsIMutI | CAGTG | 9 cut(s) | 262, 329, 372, 423, 640, 811, 1326, 1606, 1644 |
| BveI | ACCTGC | 1 cut(s) | 582 |
| Cac8I | GCNNGC | 1 cut(s) | 399 |
| CaiI | CAGNNNCTG | 1 cut(s) | 731 |
| Cfr13I | GGNCC | 2 cut(s) | 185, 305 |
| Csp6I | GTAC | 4 cut(s) | 313, 520, 1000, 1671 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1648, 1683 |
| CviQI | GTAC | 4 cut(s) | 313, 520, 1000, 1671 |
| DdeI | CTNAG | 2 cut(s) | 1217, 1677 |
| DpnI | GATC | 5 cut(s) | 894, 1125, 1462, 1494, 1518 |
| DpnII | GATC | 5 cut(s) | 892, 1123, 1460, 1492, 1516 |
| DraIII | CACNNNGTG | 1 cut(s) | 324 |
| EaeI | YGGCCR | 1 cut(s) | 853 |
| Eam1104I | CTCTTC | 2 cut(s) | 996, 1174 |
| EarI | CTCTTC | 2 cut(s) | 996, 1174 |
| Eco130I | CCWWGG | 3 cut(s) | 238, 1333, 1387 |
| Eco24I | GRGCYC | 1 cut(s) | 1412 |
| Eco31I | GGTCTC | 1 cut(s) | 491 |
| Eco32I | GATATC | 1 cut(s) | 532 |
| Eco47I | GGWCC | 2 cut(s) | 185, 305 |
| Eco57I | CTGAAG | 1 cut(s) | 396 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1228 |
| EcoRII | CCWGG | 7 cut(s) | 181, 337, 515, 610, 850, 1141, 1588 |
| EcoRV | GATATC | 1 cut(s) | 532 |
| EcoT14I | CCWWGG | 3 cut(s) | 238, 1333, 1387 |
| EcoT22I | ATGCAT | 2 cut(s) | 1431, 1437 |
| EcoT38I | GRGCYC | 1 cut(s) | 1412 |
| ErhI | CCWWGG | 3 cut(s) | 238, 1333, 1387 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 143, 175 |
| FaqI | GGGAC | 3 cut(s) | 1061, 1351, 1405 |
| FauNDI | CATATG | 1 cut(s) | 1431 |
| FblI | GTMKAC | 1 cut(s) | 1693 |
| Fnu4HI | GCNGC | 3 cut(s) | 870, 1014, 1017 |
| FokI | GGATG | 7 cut(s) | 499, 500, 572, 836, 1125, 1407, 1502 |
| FriOI | GRGCYC | 1 cut(s) | 1412 |
| Fsp4HI | GCNGC | 3 cut(s) | 870, 1014, 1017 |
| FspBI | CTAG | 4 cut(s) | 80, 542, 758, 873 |
| GluI | GCNGC | 3 cut(s) | 870, 1014, 1017 |
| GsaI | CCCAGC | 1 cut(s) | 1703 |
| GsuI | CTGGAG | 1 cut(s) | 616 |
| HaeIII | GGCC | 2 cut(s) | 756, 855 |
| HapII | CCGG | 2 cut(s) | 485, 1036 |
| HincII | GTYRAC | 3 cut(s) | 661, 694, 1694 |
| HindII | GTYRAC | 3 cut(s) | 661, 694, 1694 |
| HindIII | AAGCTT | 1 cut(s) | 1045 |
| HinfI | GANTC | 2 cut(s) | 1367, 1733 |
| HpaII | CCGG | 2 cut(s) | 485, 1036 |
| HphI | GGTGA | 5 cut(s) | 64, 124, 736, 1021, 1357 |
| Hpy166II | GTNNAC | 9 cut(s) | 72, 169, 220, 661, 694, 1136, 1249, 1326, 1694 |
| Hpy188I | TCNGA | 4 cut(s) | 301, 586, 1571, 1678 |
| Hpy188III | TCNNGA | 5 cut(s) | 188, 308, 896, 1265, 1660 |
| Hpy8I | GTNNAC | 9 cut(s) | 72, 169, 220, 661, 694, 1136, 1249, 1326, 1694 |
| Hpy99I | CGWCG | 1 cut(s) | 1695 |
| HpyAV | CCTTC | 5 cut(s) | 80, 374, 984, 1287, 1399 |
| HpyCH4III | ACNGT | 7 cut(s) | 217, 427, 748, 1111, 1330, 1610, 1751 |
| HpyCH4IV | ACGT | 2 cut(s) | 222, 1452 |
| HpyCH4V | TGCA | 6 cut(s) | 97, 512, 725, 1013, 1429, 1435 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 815 |
| HpyF3I | CTNAG | 2 cut(s) | 1217, 1677 |
| HpySE526I | ACGT | 2 cut(s) | 222, 1452 |
| Kzo9I | GATC | 5 cut(s) | 892, 1123, 1460, 1492, 1516 |
| LmnI | GCTCC | 2 cut(s) | 635, 764 |
| Lsp1109I | GCAGC | 3 cut(s) | 856, 1025, 1028 |
| LweI | GCATC | 4 cut(s) | 165, 521, 577, 977 |
| MaeI | CTAG | 4 cut(s) | 80, 542, 758, 873 |
| MaeII | ACGT | 2 cut(s) | 222, 1452 |
| MaeIII | GTNAC | 5 cut(s) | 901, 925, 1087, 1528, 1751 |
| MalI | GATC | 5 cut(s) | 894, 1125, 1462, 1494, 1518 |
| MboI | GATC | 5 cut(s) | 892, 1123, 1460, 1492, 1516 |
| MflI | RGATCY | 2 cut(s) | 892, 1460 |
| MhlI | GDGCHC | 1 cut(s) | 1412 |
| MlsI | TGGCCA | 1 cut(s) | 855 |
| MluNI | TGGCCA | 1 cut(s) | 855 |
| MlyI | GAGTC | 1 cut(s) | 1376 |
| MmeI | TCCRAC | 2 cut(s) | 1007, 1438 |
| Mox20I | TGGCCA | 1 cut(s) | 855 |
| Mph1103I | ATGCAT | 2 cut(s) | 1431, 1437 |
| MroXI | GAANNNNTTC | 1 cut(s) | 128 |
| MscI | TGGCCA | 1 cut(s) | 855 |
| MseI | TTAA | 6 cut(s) | 225, 1041, 1082, 1197, 1416, 1506 |
| MslI | CAYNNNNRTG | 5 cut(s) | 102, 267, 585, 921, 1434 |
| Msp20I | TGGCCA | 1 cut(s) | 855 |
| MspI | CCGG | 2 cut(s) | 485, 1036 |
| MspR9I | CCNGG | 8 cut(s) | 183, 339, 517, 612, 852, 1036, 1143, 1590 |
| MvaI | CCWGG | 7 cut(s) | 183, 339, 517, 612, 852, 1143, 1590 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 815 |
| NciI | CCSGG | 1 cut(s) | 1036 |
| NdeI | CATATG | 1 cut(s) | 1431 |
| NdeII | GATC | 5 cut(s) | 892, 1123, 1460, 1492, 1516 |
| NlaIV | GGNNCC | 3 cut(s) | 463, 766, 894 |
| NmuCI | GTSAC | 1 cut(s) | 901 |
| NsiI | ATGCAT | 2 cut(s) | 1431, 1437 |
| NspI | RCATGY | 2 cut(s) | 401, 1711 |
| PaeI | GCATGC | 1 cut(s) | 401 |
| PasI | CCCWGGG | 1 cut(s) | 1589 |
| PdmI | GAANNNNTTC | 1 cut(s) | 128 |
| PfeI | GAWTC | 1 cut(s) | 1733 |
| PfoI | TCCNGGA | 2 cut(s) | 337, 1034 |
| PkrI | GCNGC | 3 cut(s) | 871, 1015, 1018 |
| PleI | GAGTC | 1 cut(s) | 1375 |
| PpsI | GAGTC | 1 cut(s) | 1375 |
| Ppu21I | YACGTR | 1 cut(s) | 1453 |
| PshAI | GACNNNNGTC | 1 cut(s) | 621 |
| PsiI | TTATAA | 4 cut(s) | 506, 1106, 1287, 1362 |
| Psp6I | CCWGG | 7 cut(s) | 181, 337, 515, 610, 850, 1141, 1588 |
| PspFI | CCCAGC | 1 cut(s) | 1699 |
| PspGI | CCWGG | 7 cut(s) | 181, 337, 515, 610, 850, 1141, 1588 |
| PspN4I | GGNNCC | 3 cut(s) | 463, 766, 894 |
| PspPI | GGNCC | 2 cut(s) | 185, 305 |
| PstNI | CAGNNNCTG | 1 cut(s) | 731 |
| PsuI | RGATCY | 2 cut(s) | 892, 1460 |
| RsaI | GTAC | 4 cut(s) | 314, 521, 1001, 1672 |
| RsaNI | GTAC | 4 cut(s) | 313, 520, 1000, 1671 |
| RseI | CAYNNNNRTG | 5 cut(s) | 102, 267, 585, 921, 1434 |
| SalI | GTCGAC | 1 cut(s) | 1692 |
| SaqAI | TTAA | 6 cut(s) | 225, 1041, 1082, 1197, 1416, 1506 |
| SatI | GCNGC | 3 cut(s) | 870, 1014, 1017 |
| Sau3AI | GATC | 5 cut(s) | 892, 1123, 1460, 1492, 1516 |
| Sau96I | GGNCC | 2 cut(s) | 185, 305 |
| SchI | GAGTC | 1 cut(s) | 1376 |
| ScrFI | CCNGG | 8 cut(s) | 183, 339, 517, 612, 852, 1036, 1143, 1590 |
| SduI | GDGCHC | 1 cut(s) | 1412 |
| SfaNI | GCATC | 4 cut(s) | 165, 521, 577, 977 |
| SfcI | CTRYAG | 2 cut(s) | 1068, 1228 |
| SinI | GGWCC | 2 cut(s) | 185, 305 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 102, 267, 585, 921, 1434 |
| SmlI | CTYRAG | 2 cut(s) | 883, 1627 |
| SmoI | CTYRAG | 2 cut(s) | 883, 1627 |
| SpeI | ACTAGT | 1 cut(s) | 541 |
| SphI | GCATGC | 1 cut(s) | 401 |
| SsiI | CCGC | 3 cut(s) | 67, 930, 1274 |
| SspI | AATATT | 2 cut(s) | 1318, 1762 |
| SspMI | CTAG | 4 cut(s) | 80, 542, 758, 873 |
| StyD4I | CCNGG | 8 cut(s) | 181, 337, 515, 610, 850, 1034, 1141, 1588 |
| StyI | CCWWGG | 3 cut(s) | 238, 1333, 1387 |
| TaaI | ACNGT | 7 cut(s) | 217, 427, 748, 1111, 1330, 1610, 1751 |
| TaiI | ACGT | 2 cut(s) | 225, 1455 |
| TaqI | TCGA | 3 cut(s) | 23, 652, 1693 |
| TatI | WGTACW | 2 cut(s) | 312, 999 |
| TfiI | GAWTC | 1 cut(s) | 1733 |
| Tru1I | TTAA | 6 cut(s) | 225, 1041, 1082, 1197, 1416, 1506 |
| Tru9I | TTAA | 6 cut(s) | 225, 1041, 1082, 1197, 1416, 1506 |
| TscAI | CASTG | 9 cut(s) | 269, 329, 379, 430, 640, 811, 1333, 1613, 1651 |
| TseFI | GTSAC | 1 cut(s) | 901 |
| TseI | GCWGC | 3 cut(s) | 869, 1013, 1016 |
| Tsp45I | GTSAC | 1 cut(s) | 901 |
| TspGWI | ACGGA | 3 cut(s) | 282, 777, 1271 |
| TspRI | CASTG | 9 cut(s) | 269, 329, 379, 430, 640, 811, 1333, 1613, 1651 |
| VpaK11BI | GGWCC | 2 cut(s) | 185, 305 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1228 |
| XapI | RAATTY | 3 cut(s) | 39, 346, 1025 |
| XceI | RCATGY | 2 cut(s) | 401, 1711 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 245, 291 |
| XmiI | GTMKAC | 1 cut(s) | 1693 |
| XmnI | GAANNNNTTC | 1 cut(s) | 128 |
| XspI | CTAG | 4 cut(s) | 80, 542, 758, 873 |
| Zsp2I | ATGCAT | 2 cut(s) | 1431, 1437 |