Lignin degradation and detoxification of lignin-derived products
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 1473 |
| Acc36I | ACCTGC | 1 cut(s) | 1473 |
| AccB1I | GGYRCC | 1 cut(s) | 452 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 282, 1586 |
| AccBSI | CCGCTC | 1 cut(s) | 85 |
| AccI | GTMKAC | 1 cut(s) | 41 |
| AccII | CGCG | 1 cut(s) | 1790 |
| AciI | CCGC | 4 cut(s) | 83, 1140, 1307, 1790 |
| AclI | AACGTT | 1 cut(s) | 1568 |
| AclWI | GGATC | 3 cut(s) | 538, 1343, 1544 |
| AcoI | YGGCCR | 1 cut(s) | 628 |
| AcsI | RAATTY | 3 cut(s) | 364, 547, 865 |
| AcuI | CTGAAG | 1 cut(s) | 966 |
| AfaI | GTAC | 4 cut(s) | 33, 403, 1127, 1159 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 282, 476, 772, 1169, 1586, 1732 |
| AflIII | ACRYGT | 1 cut(s) | 849 |
| AgsI | TTSAA | 6 cut(s) | 553, 959, 1042, 1367, 1421, 1540 |
| AhlI | ACTAGT | 1 cut(s) | 23 |
| AjiI | CACGTC | 1 cut(s) | 850 |
| AjnI | CCWGG | 3 cut(s) | 199, 832, 1621 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 472, 504 |
| Alw21I | GWGCWC | 1 cut(s) | 220 |
| Alw26I | GTCTC | 2 cut(s) | 1648, 1706 |
| AlwI | GGATC | 3 cut(s) | 538, 1343, 1544 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 52, 932 |
| Ama87I | CYCGRG | 1 cut(s) | 102 |
| AoxI | GGCC | 2 cut(s) | 322, 628 |
| ApeKI | GCWGC | 3 cut(s) | 62, 608, 1199 |
| ApoI | RAATTY | 3 cut(s) | 364, 547, 865 |
| AseI | ATTAAT | 2 cut(s) | 624, 1800 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 1008, 1511 |
| AspS9I | GGNCC | 4 cut(s) | 203, 323, 577, 1172 |
| AsuC2I | CCSGG | 1 cut(s) | 357 |
| AsuHPI | GGTGA | 2 cut(s) | 265, 345 |
| AvaI | CYCGRG | 1 cut(s) | 102 |
| AvaII | GGWCC | 3 cut(s) | 203, 577, 1172 |
| BaeGI | GKGCMC | 1 cut(s) | 1706 |
| BanI | GGYRCC | 1 cut(s) | 452 |
| BanII | GRGCYC | 1 cut(s) | 220 |
| Bbv12I | GWGCWC | 1 cut(s) | 220 |
| BbvI | GCAGC | 3 cut(s) | 49, 620, 1186 |
| BccI | CCATC | 5 cut(s) | 314, 812, 1405, 1429, 1584 |
| BceAI | ACGGC | 1 cut(s) | 802 |
| BciT130I | CCWGG | 3 cut(s) | 201, 834, 1623 |
| BciVI | GTATCC | 2 cut(s) | 223, 1092 |
| BcnI | CCSGG | 1 cut(s) | 357 |
| BcoDI | GTCTC | 2 cut(s) | 1648, 1706 |
| BcuI | ACTAGT | 1 cut(s) | 23 |
| BfaI | CTAG | 4 cut(s) | 24, 153, 435, 1388 |
| BfmI | CTRYAG | 2 cut(s) | 915, 1048 |
| BfuAI | ACCTGC | 1 cut(s) | 1473 |
| BfuI | GTATCC | 2 cut(s) | 223, 1092 |
| BisI | GCNGC | 3 cut(s) | 63, 609, 1200 |
| BlpI | GCTNAGC | 1 cut(s) | 1066 |
| BlsI | GCNGC | 3 cut(s) | 64, 610, 1201 |
| BmcAI | AGTACT | 1 cut(s) | 33 |
| Bme1390I | CCNGG | 4 cut(s) | 201, 357, 834, 1623 |
| Bme18I | GGWCC | 3 cut(s) | 203, 577, 1172 |
| BmeT110I | CYCGRG | 1 cut(s) | 102 |
| BmgBI | CACGTC | 1 cut(s) | 850 |
| BmgT120I | GGNCC | 4 cut(s) | 203, 323, 577, 1172 |
| BmiI | GGNNCC | 5 cut(s) | 454, 579, 790, 1174, 1444 |
| BmrFI | CCNGG | 4 cut(s) | 201, 357, 834, 1623 |
| BmsI | GCATC | 2 cut(s) | 931, 938 |
| BoxI | GACNNNNGTC | 2 cut(s) | 570, 1376 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1511, 1543 |
| BpmI | CTGGAG | 1 cut(s) | 1146 |
| Bpu10I | CCTNAGC | 1 cut(s) | 498 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1066 |
| BpuMI | CCSGG | 1 cut(s) | 357 |
| Bsa29I | ATCGAT | 2 cut(s) | 729, 1796 |
| BsaJI | CCNNGG | 3 cut(s) | 311, 832, 1232 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1431, 1461 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 282, 476, 772, 1169, 1586, 1732 |
| Bse118I | RCCGGY | 2 cut(s) | 630, 1276 |
| Bse1I | ACTGG | 4 cut(s) | 29, 287, 434, 1352 |
| Bse3DI | GCAATG | 1 cut(s) | 750 |
| BseBI | CCWGG | 3 cut(s) | 201, 834, 1623 |
| BseCI | ATCGAT | 2 cut(s) | 729, 1796 |
| BseDI | CCNNGG | 3 cut(s) | 311, 832, 1232 |
| BseGI | GGATG | 3 cut(s) | 75, 1453, 1595 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 282, 476, 772, 1169, 1586, 1732 |
| BseMI | GCAATG | 1 cut(s) | 750 |
| BseMII | CTCAG | 3 cut(s) | 489, 1615, 1701 |
| BseNI | ACTGG | 4 cut(s) | 29, 287, 434, 1352 |
| BseRI | GAGGAG | 1 cut(s) | 527 |
| BseSI | GKGCMC | 1 cut(s) | 1706 |
| BseXI | GCAGC | 3 cut(s) | 49, 620, 1186 |
| Bsh1236I | CGCG | 1 cut(s) | 1790 |
| BshFI | GGCC | 2 cut(s) | 324, 630 |
| BshNI | GGYRCC | 1 cut(s) | 452 |
| BshVI | ATCGAT | 2 cut(s) | 729, 1796 |
| BsiHKAI | GWGCWC | 1 cut(s) | 220 |
| BsiHKCI | CYCGRG | 1 cut(s) | 102 |
| BsiSI | CCGG | 4 cut(s) | 356, 631, 1277, 1733 |
| BslFI | GGGAC | 4 cut(s) | 1079, 1158, 1384, 1538 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 282, 476, 772, 1169, 1586, 1732 |
| BsmAI | GTCTC | 2 cut(s) | 1648, 1706 |
| BsmFI | GGGAC | 4 cut(s) | 1079, 1158, 1384, 1538 |
| BsmI | GAATGC | 1 cut(s) | 1037 |
| BsnI | GGCC | 2 cut(s) | 324, 630 |
| BsoBI | CYCGRG | 1 cut(s) | 102 |
| Bsp1286I | GDGCHC | 2 cut(s) | 220, 1706 |
| Bsp1407I | TGTACA | 1 cut(s) | 1125 |
| Bsp143I | GATC | 4 cut(s) | 516, 530, 1348, 1549 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1066 |
| Bsp19I | CCATGG | 1 cut(s) | 311 |
| BspACI | CCGC | 4 cut(s) | 83, 1140, 1307, 1790 |
| BspANI | GGCC | 2 cut(s) | 324, 630 |
| BspCNI | CTCAG | 3 cut(s) | 490, 1614, 1702 |
| BspDI | ATCGAT | 2 cut(s) | 729, 1796 |
| BspFNI | CGCG | 1 cut(s) | 1790 |
| BspLI | GGNNCC | 5 cut(s) | 454, 579, 790, 1174, 1444 |
| BspMI | ACCTGC | 1 cut(s) | 1473 |
| BspPI | GGATC | 3 cut(s) | 538, 1343, 1544 |
| BspQI | GCTCTTC | 1 cut(s) | 92 |
| BspT107I | GGYRCC | 1 cut(s) | 452 |
| BsrBI | CCGCTC | 1 cut(s) | 85 |
| BsrDI | GCAATG | 1 cut(s) | 750 |
| BsrFI | RCCGGY | 2 cut(s) | 630, 1276 |
| BsrGI | TGTACA | 1 cut(s) | 1125 |
| BsrI | ACTGG | 4 cut(s) | 29, 287, 434, 1352 |
| BssAI | RCCGGY | 2 cut(s) | 630, 1276 |
| BssECI | CCNNGG | 3 cut(s) | 311, 832, 1232 |
| BssMI | GATC | 4 cut(s) | 516, 530, 1348, 1549 |
| BssT1I | CCWWGG | 1 cut(s) | 311 |
| Bst2UI | CCWGG | 3 cut(s) | 201, 834, 1623 |
| Bst4CI | ACNGT | 7 cut(s) | 48, 452, 772, 919, 1157, 1363, 1405 |
| Bst6I | CTCTTC | 3 cut(s) | 92, 504, 892 |
| BstAUI | TGTACA | 1 cut(s) | 1125 |
| BstC8I | GCNNGC | 7 cut(s) | 417, 442, 471, 632, 1204, 1251, 1274 |
| BstDEI | CTNAG | 6 cut(s) | 498, 908, 1066, 1601, 1710, 1781 |
| BstDSI | CCRYGG | 1 cut(s) | 311 |
| BstF5I | GGATG | 3 cut(s) | 75, 1453, 1595 |
| BstFNI | CGCG | 1 cut(s) | 1790 |
| BstKTI | GATC | 4 cut(s) | 519, 533, 1351, 1552 |
| BstMAI | GTCTC | 2 cut(s) | 1648, 1706 |
| BstMBI | GATC | 4 cut(s) | 516, 530, 1348, 1549 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 215, 786 |
| BstNI | CCWGG | 3 cut(s) | 201, 834, 1623 |
| BstNSI | RCATGY | 2 cut(s) | 419, 1182 |
| BstPAI | GACNNNNGTC | 2 cut(s) | 570, 1376 |
| BstSCI | CCNGG | 4 cut(s) | 199, 355, 832, 1621 |
| BstSFI | CTRYAG | 2 cut(s) | 915, 1048 |
| BstSLI | GKGCMC | 1 cut(s) | 1706 |
| BstUI | CGCG | 1 cut(s) | 1790 |
| BstV1I | GCAGC | 3 cut(s) | 49, 620, 1186 |
| BstX2I | RGATCY | 1 cut(s) | 530 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1629 |
| BstYI | RGATCY | 1 cut(s) | 530 |
| Bsu15I | ATCGAT | 2 cut(s) | 729, 1796 |
| BsuI | GTATCC | 2 cut(s) | 223, 1092 |
| BsuRI | GGCC | 2 cut(s) | 324, 630 |
| BsuTUI | ATCGAT | 2 cut(s) | 729, 1796 |
| BtgI | CCRYGG | 1 cut(s) | 311 |
| BtgZI | GCGATG | 1 cut(s) | 1352 |
| BtrI | CACGTC | 1 cut(s) | 850 |
| BtsCI | GGATG | 3 cut(s) | 75, 1453, 1595 |
| BtsIMutI | CAGTG | 4 cut(s) | 280, 768, 1326, 1359 |
| BveI | ACCTGC | 1 cut(s) | 1473 |
| Cac8I | GCNNGC | 7 cut(s) | 417, 442, 471, 632, 1204, 1251, 1274 |
| CaiI | CAGNNNCTG | 2 cut(s) | 52, 932 |
| Cfr10I | RCCGGY | 2 cut(s) | 630, 1276 |
| Cfr13I | GGNCC | 4 cut(s) | 203, 323, 577, 1172 |
| ClaI | ATCGAT | 2 cut(s) | 729, 1796 |
| CseI | GACGC | 2 cut(s) | 1171, 1195 |
| Csp6I | GTAC | 4 cut(s) | 32, 402, 1126, 1158 |
| CviQI | GTAC | 4 cut(s) | 32, 402, 1126, 1158 |
| DdeI | CTNAG | 6 cut(s) | 498, 908, 1066, 1601, 1710, 1781 |
| DpnI | GATC | 4 cut(s) | 518, 532, 1350, 1551 |
| DpnII | GATC | 4 cut(s) | 516, 530, 1348, 1549 |
| EaeI | YGGCCR | 1 cut(s) | 628 |
| Eam1104I | CTCTTC | 3 cut(s) | 92, 504, 892 |
| EarI | CTCTTC | 3 cut(s) | 92, 504, 892 |
| EciI | GGCGGA | 1 cut(s) | 1129 |
| Ecl136II | GAGCTC | 1 cut(s) | 218 |
| Eco130I | CCWWGG | 1 cut(s) | 311 |
| Eco24I | GRGCYC | 1 cut(s) | 220 |
| Eco32I | GATATC | 1 cut(s) | 1111 |
| Eco47I | GGWCC | 3 cut(s) | 203, 577, 1172 |
| Eco53kI | GAGCTC | 1 cut(s) | 218 |
| Eco57I | CTGAAG | 1 cut(s) | 966 |
| Eco88I | CYCGRG | 1 cut(s) | 102 |
| EcoICRI | GAGCTC | 1 cut(s) | 218 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1172 |
| EcoRII | CCWGG | 3 cut(s) | 199, 832, 1621 |
| EcoRV | GATATC | 1 cut(s) | 1111 |
| EcoT14I | CCWWGG | 1 cut(s) | 311 |
| EcoT38I | GRGCYC | 1 cut(s) | 220 |
| ErhI | CCWWGG | 1 cut(s) | 311 |
| FaqI | GGGAC | 4 cut(s) | 1079, 1158, 1384, 1538 |
| FauNDI | CATATG | 1 cut(s) | 1647 |
| FblI | GTMKAC | 1 cut(s) | 41 |
| Fnu4HI | GCNGC | 3 cut(s) | 63, 609, 1200 |
| FokI | GGATG | 3 cut(s) | 62, 1440, 1602 |
| FriOI | GRGCYC | 1 cut(s) | 220 |
| Fsp4HI | GCNGC | 3 cut(s) | 63, 609, 1200 |
| FspBI | CTAG | 4 cut(s) | 24, 153, 435, 1388 |
| GluI | GCNGC | 3 cut(s) | 63, 609, 1200 |
| GsuI | CTGGAG | 1 cut(s) | 1146 |
| HaeIII | GGCC | 2 cut(s) | 324, 630 |
| HapII | CCGG | 4 cut(s) | 356, 631, 1277, 1733 |
| HgaI | GACGC | 2 cut(s) | 1171, 1195 |
| HincII | GTYRAC | 4 cut(s) | 42, 387, 565, 1359 |
| HindII | GTYRAC | 4 cut(s) | 42, 387, 565, 1359 |
| HindIII | AAGCTT | 1 cut(s) | 1063 |
| HpaI | GTTAAC | 1 cut(s) | 1359 |
| HpaII | CCGG | 4 cut(s) | 356, 631, 1277, 1733 |
| HphI | GGTGA | 2 cut(s) | 265, 345 |
| Hpy188I | TCNGA | 8 cut(s) | 530, 711, 896, 934, 985, 1061, 1233, 1604 |
| Hpy188III | TCNNGA | 5 cut(s) | 497, 575, 1001, 1013, 1163 |
| Hpy99I | CGWCG | 1 cut(s) | 678 |
| HpyAV | CCTTC | 3 cut(s) | 392, 1546, 1755 |
| HpyCH4III | ACNGT | 7 cut(s) | 48, 452, 772, 919, 1157, 1363, 1405 |
| HpyCH4IV | ACGT | 3 cut(s) | 668, 849, 1568 |
| HpyCH4V | TGCA | 6 cut(s) | 584, 950, 1202, 1249, 1462, 1468 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 215, 786 |
| HpyF3I | CTNAG | 6 cut(s) | 498, 908, 1066, 1601, 1710, 1781 |
| HpySE526I | ACGT | 3 cut(s) | 668, 849, 1568 |
| KroI | GCCGGC | 1 cut(s) | 630 |
| KroNI | GCCGGC | 1 cut(s) | 632 |
| KspAI | GTTAAC | 1 cut(s) | 1359 |
| Kzo9I | GATC | 4 cut(s) | 516, 530, 1348, 1549 |
| LguI | GCTCTTC | 1 cut(s) | 92 |
| LmnI | GCTCC | 5 cut(s) | 659, 794, 879, 994, 1728 |
| Lsp1109I | GCAGC | 3 cut(s) | 49, 620, 1186 |
| LweI | GCATC | 2 cut(s) | 931, 938 |
| MaeI | CTAG | 4 cut(s) | 24, 153, 435, 1388 |
| MaeII | ACGT | 3 cut(s) | 668, 849, 1568 |
| MaeIII | GTNAC | 4 cut(s) | 334, 425, 614, 1784 |
| MalI | GATC | 4 cut(s) | 518, 532, 1350, 1551 |
| MbiI | CCGCTC | 1 cut(s) | 85 |
| MboI | GATC | 4 cut(s) | 516, 530, 1348, 1549 |
| MboII | GAAGA | 8 cut(s) | 25, 79, 407, 521, 695, 698, 909, 1705 |
| MflI | RGATCY | 1 cut(s) | 530 |
| MhlI | GDGCHC | 2 cut(s) | 220, 1706 |
| MluCI | AATT | 7 cut(s) | 234, 364, 547, 621, 865, 1507, 1540 |
| MlyI | GAGTC | 2 cut(s) | 1520, 1685 |
| MmeI | TCCRAC | 1 cut(s) | 874 |
| MnlI | CCTC | 8 cut(s) | 133, 505, 776, 1227, 1433, 1515, 1563, 1740 |
| MroNI | GCCGGC | 1 cut(s) | 630 |
| MroXI | GAANNNNTTC | 2 cut(s) | 1008, 1511 |
| MseI | TTAA | 6 cut(s) | 135, 243, 624, 1320, 1358, 1800 |
| MslI | CAYNNNNRTG | 2 cut(s) | 285, 1467 |
| MspA1I | CMGCKG | 1 cut(s) | 611 |
| MspI | CCGG | 4 cut(s) | 356, 631, 1277, 1733 |
| MspR9I | CCNGG | 4 cut(s) | 201, 357, 834, 1623 |
| Mva1269I | GAATGC | 1 cut(s) | 1037 |
| MvaI | CCWGG | 3 cut(s) | 201, 834, 1623 |
| MvnI | CGCG | 1 cut(s) | 1790 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 215, 786 |
| NaeI | GCCGGC | 1 cut(s) | 632 |
| NciI | CCSGG | 1 cut(s) | 357 |
| NcoI | CCATGG | 1 cut(s) | 311 |
| NdeI | CATATG | 1 cut(s) | 1647 |
| NdeII | GATC | 4 cut(s) | 516, 530, 1348, 1549 |
| NgoMIV | GCCGGC | 1 cut(s) | 630 |
| NlaIV | GGNNCC | 5 cut(s) | 454, 579, 790, 1174, 1444 |
| NmeAIII | GCCGAG | 1 cut(s) | 1543 |
| NmuCI | GTSAC | 3 cut(s) | 334, 425, 1784 |
| NspI | RCATGY | 2 cut(s) | 419, 1182 |
| PaeI | GCATGC | 1 cut(s) | 419 |
| PaeR7I | CTCGAG | 1 cut(s) | 102 |
| PaqCI | CACCTGC | 1 cut(s) | 1473 |
| PciSI | GCTCTTC | 1 cut(s) | 92 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 726 |
| PctI | GAATGC | 1 cut(s) | 1037 |
| PdiI | GCCGGC | 1 cut(s) | 632 |
| PdmI | GAANNNNTTC | 2 cut(s) | 1008, 1511 |
| PfeI | GAWTC | 8 cut(s) | 164, 1009, 1016, 1238, 1450, 1605, 1766, 1793 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 282, 1586 |
| PkrI | GCNGC | 3 cut(s) | 64, 610, 1201 |
| PleI | GAGTC | 2 cut(s) | 1520, 1684 |
| PpsI | GAGTC | 2 cut(s) | 1520, 1684 |
| PpuMI | RGGWCCY | 1 cut(s) | 1172 |
| PshAI | GACNNNNGTC | 2 cut(s) | 570, 1376 |
| PshBI | ATTAAT | 2 cut(s) | 624, 1800 |
| Psp124BI | GAGCTC | 1 cut(s) | 220 |
| Psp1406I | AACGTT | 1 cut(s) | 1568 |
| Psp5II | RGGWCCY | 1 cut(s) | 1172 |
| Psp6I | CCWGG | 3 cut(s) | 199, 832, 1621 |
| PspGI | CCWGG | 3 cut(s) | 199, 832, 1621 |
| PspN4I | GGNNCC | 5 cut(s) | 454, 579, 790, 1174, 1444 |
| PspPI | GGNCC | 4 cut(s) | 203, 323, 577, 1172 |
| PspPPI | RGGWCCY | 1 cut(s) | 1172 |
| PspXI | VCTCGAGB | 1 cut(s) | 102 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 352, 384 |
| PstNI | CAGNNNCTG | 2 cut(s) | 52, 932 |
| PsuI | RGATCY | 1 cut(s) | 530 |
| PvuII | CAGCTG | 1 cut(s) | 611 |
| RsaI | GTAC | 4 cut(s) | 33, 403, 1127, 1159 |
| RsaNI | GTAC | 4 cut(s) | 32, 402, 1126, 1158 |
| RseI | CAYNNNNRTG | 2 cut(s) | 285, 1467 |
| SacI | GAGCTC | 1 cut(s) | 220 |
| SalI | GTCGAC | 1 cut(s) | 40 |
| SapI | GCTCTTC | 1 cut(s) | 92 |
| SaqAI | TTAA | 6 cut(s) | 135, 243, 624, 1320, 1358, 1800 |
| SatI | GCNGC | 3 cut(s) | 63, 609, 1200 |
| Sau3AI | GATC | 4 cut(s) | 516, 530, 1348, 1549 |
| Sau96I | GGNCC | 4 cut(s) | 203, 323, 577, 1172 |
| ScaI | AGTACT | 1 cut(s) | 33 |
| SchI | GAGTC | 2 cut(s) | 1520, 1685 |
| ScrFI | CCNGG | 4 cut(s) | 201, 357, 834, 1623 |
| SduI | GDGCHC | 2 cut(s) | 220, 1706 |
| SfaNI | GCATC | 2 cut(s) | 931, 938 |
| SfcI | CTRYAG | 2 cut(s) | 915, 1048 |
| Sfr274I | CTCGAG | 1 cut(s) | 102 |
| SinI | GGWCC | 3 cut(s) | 203, 577, 1172 |
| SlaI | CTCGAG | 1 cut(s) | 102 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 285, 1467 |
| SmlI | CTYRAG | 1 cut(s) | 102 |
| SmoI | CTYRAG | 1 cut(s) | 102 |
| SpeI | ACTAGT | 1 cut(s) | 23 |
| SphI | GCATGC | 1 cut(s) | 419 |
| Sse9I | AATT | 7 cut(s) | 234, 364, 547, 621, 865, 1507, 1540 |
| SsiI | CCGC | 4 cut(s) | 83, 1140, 1307, 1790 |
| SspMI | CTAG | 4 cut(s) | 24, 153, 435, 1388 |
| SstI | GAGCTC | 1 cut(s) | 220 |
| StyD4I | CCNGG | 4 cut(s) | 199, 355, 832, 1621 |
| StyI | CCWWGG | 1 cut(s) | 311 |
| TaaI | ACNGT | 7 cut(s) | 48, 452, 772, 919, 1157, 1363, 1405 |
| TaiI | ACGT | 3 cut(s) | 671, 852, 1571 |
| TaqI | TCGA | 6 cut(s) | 41, 103, 729, 1347, 1764, 1796 |
| TasI | AATT | 7 cut(s) | 234, 364, 547, 621, 865, 1507, 1540 |
| TatI | WGTACW | 3 cut(s) | 31, 1125, 1157 |
| TfiI | GAWTC | 8 cut(s) | 164, 1009, 1016, 1238, 1450, 1605, 1766, 1793 |
| Tru1I | TTAA | 6 cut(s) | 135, 243, 624, 1320, 1358, 1800 |
| Tru9I | TTAA | 6 cut(s) | 135, 243, 624, 1320, 1358, 1800 |
| TscAI | CASTG | 4 cut(s) | 287, 775, 1333, 1359 |
| TseFI | GTSAC | 3 cut(s) | 334, 425, 1784 |
| TseI | GCWGC | 3 cut(s) | 62, 608, 1199 |
| Tsp45I | GTSAC | 3 cut(s) | 334, 425, 1784 |
| TspDTI | ATGAA | 9 cut(s) | 17, 26, 79, 104, 522, 1299, 1317, 1413, 1561 |
| TspGWI | ACGGA | 1 cut(s) | 1435 |
| TspRI | CASTG | 4 cut(s) | 287, 775, 1333, 1359 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 282, 1586 |
| VpaK11BI | GGWCC | 3 cut(s) | 203, 577, 1172 |
| VspI | ATTAAT | 2 cut(s) | 624, 1800 |
| XapI | RAATTY | 3 cut(s) | 364, 547, 865 |
| XceI | RCATGY | 2 cut(s) | 419, 1182 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 309, 1587 |
| XhoI | CTCGAG | 1 cut(s) | 102 |
| XmiI | GTMKAC | 1 cut(s) | 41 |
| XmnI | GAANNNNTTC | 2 cut(s) | 1008, 1511 |
| XspI | CTAG | 4 cut(s) | 24, 153, 435, 1388 |
| ZrmI | AGTACT | 1 cut(s) | 33 |