Rorug07G0303000

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0000320
Physical Location & Seq
Reverse (-)
30137272 .. 30141464
4193 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0303000.1

Sequence Viewer

Length: 1503 bp
ATGGCAGAAGAAGAACTCAGCAACTCTTTAGAGTTTACTCCAACATGGGTAGTAGCAGGTGTTTGCTTTGTCATTGTTCTGTTATCTCTTTTTGCCGAGCGTGGTCTCCATAAACTTGGAAAGTATTTACTGCATGAGAAACAAGATGCACTATATGAAGCCTTACAAAAACTAAAAGAAGAATTGATGCTTTTAGGTTTCATTTCCCTTCTGCTAACTGTAAGTCAACGTTCCATAAGCCGCATATGCATCCCTGCCCATGTCGCAAATCATATGCTACCATGTAAGAGGGAAACTGCTGAGCACAAAACCCCTGAACATTACTTTCTTAATCACTCTACAAACAATGGTCGACGCCTTCTGTCTTCAGAAACAACTTCCAATCACTGTCAGCTCAAGGGGAAGGTTCCATTGTTATCATTGGAAGGATTACATCATCTACACATCTTCATCTTTGTATTAGCAGTTGTTCATGTGATCTTCTGTGTCTCAACCATGGTTCTTGGAGGGGCAAGAATACGACAATGGAAGCGCTGGGAGGATTCCATCCAGAAGGATCGAAGAAGTGGTCAAAGTACTGCTCACGTTCGTCATCACCATCATGAGTTCTTGAAAGAACGTGCAGATGGATATTGGAGAAGAGCAGCTATTATAGGTTGGATGATAGCATTCTTCAAACAATTTTATGGCTCTGTCACTAAGTCAGACTACATTGCACTGCGGCAAGGGTTTATCAAGGAGCACTGCCCTCGAAATCCTGATTTTGATTTTCACAAATACATGATGCGGACACTTGAAATTGATTTTAGAAAGATTGTTGGGATCACCTGGTACCTGTGGCTCTTTGTTGTGCTGTTTTTGTTGCTGAATGTGGACGGATGGAACACATACTTTTGGTTAGCATTTTTACCGCTGATTCTTCTGCTTCTAGTGGGGGCCAAATTGGAACACATAATCAGCCGTTTGGCTCAGGATGTTAAACAAACAGGAGTACATGCTAATGAATCAGCTATAAGAGTGAGGCCTTCAGATGAACACTTCTGGTTTAACAATCCTAGAATTGTTCTCAATTTGATCCACTTCATTCTGTTTCAAAATTCATTCGAGATTGCTTTCTTCTTCTGGATTTGGAGCACCTATGGAATTCACTCATGCATCATGGAAAAGTTGGGCTATGTCATCGCAAGACTTTTTATGGGTGTGATAGTTCAAGTGCTTTGCAGTTACAGCACCCTACCATTGTATGTTATCGTCACCCAGATGGGTAGCATGTTCAAGAAGGGAATGTTCGATGACACGGTACATGGTGCCCTCGATATATGGGCTGGAGACGCAATTAGTAGACGTGACGATAGCAAGCAATCCTCCTCACATAGTATGCACAAAATGATCAACAATGAAGCCTCCACAACTGTCGATGTTGCAGAACAAGAAATGGTTGTCATGGAAAGAACTATAAATGTGCTCCCTTGTCAAGTCCAGCCACCTTCTTCATCCTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00053 ko01100 map00053 map01100
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

500

Amino Acids

57.79

Weight (kDa)

8.61

Isoelectric Point (pI)

38.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mlo PF03094 8 - 468 3.3e-181 Mlo family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000320)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g12640 FvH4_2g18980 FvH4_2g18990 FvH4_2g40270 FvH4_2g40270 FvH4_4g22740 FvH4_4g22740 FvH4_5g35760 FvH4_5g35760 FvH4_5g35760 FvH4_5g35760 FvH4_5g35760 FvH4_5g35760 FvH4_5g35760 FvH4_5g35760 FvH4_5g36460 FvH4_5g36460 FvH4_6g39950 FvH4_6g39970 FvH4_6g39983 FvH4_6g39990
rosa_chinensis RchiOBHm_Chr2g0154481 RchiOBHm_Chr2g0154511 RchiOBHm_Chr4g0428961 RchiOBHm_Chr6g0252431 RchiOBHm_Chr6g0273301 RchiOBHm_Chr6g0273631 RchiOBHm_Chr6g0273651 RchiOBHm_Chr6g0284751 RchiOBHm_Chr6g0284761 RchiOBHm_Chr6g0300491 RchiOBHm_Chr7g0237421 RchiOBHm_Chr7g0238581
rosa_laevigata RLG00000000906 RLG00000001006 RLG00000007110 RLG00000011333 RLG00000012721 RLG00000013605 RLG00000013626 RLG00000020780 RLG00000020782 RLG00000020783 RLG00000020786
rosa_multiflora Rmu_co8321753.1_g000001 Rmu_sc0000239.1_g000020 Rmu_sc0000686.1_g000001 Rmu_sc0000686.1_g000003 Rmu_sc0000686.1_g000005 Rmu_sc0001476.1_g000011 Rmu_sc0002231.1_g000002 Rmu_sc0002231.1_g000016 Rmu_sc0002690.1_g000003 Rmu_sc0002717.1_g000015 Rmu_sc0002923.1_g000026 Rmu_sc0003808.1_g000016 Rmu_sc0006475.1_g000008 Rmu_sc0006475.1_g000012 Rmu_sc0006475.1_g000018 Rmu_sc0014815.1_g000004 Rmu_sc0015313.1_g000012
rosa_roxburghii Rroxscaffold_2G00094270 Rroxscaffold_2G00094290 Rroxscaffold_3G00222950 Rroxscaffold_3G00224250 Rroxscaffold_5G00370630 Rroxscaffold_7G00167740 Rroxscaffold_7G00184410 Rroxscaffold_7G00195190 Rroxscaffold_7G00195460
rosa_rugosa Rorug02G0444600 Rorug04G0231100 Rorug04G0231200 Rorug06G0074000 Rorug06G0076800 Rorug06G0166400 Rorug06G0166500 Rorug06G0166600 Rorug06G0296400 Rorug07G0303000
rosa_samantha Rh2CG494600 Rh2CG494700 Rh2DG530800 Rh2DG530900 Rh2DG531000 Rh4AG287500 Rh4AG403800 Rh4BG293400 Rh4BG415100 Rh4DG290500 Rh6AG191000 Rh6AG191300 Rh6BG192500 Rh6BG194800 Rh6BG195200 Rh6BG279500 Rh6BG279600 Rh6DG183900 Rh6DG186600 Rh6DG274300 Rh6DG274500 Rh6DG409500 Rh7AG460600 Rh7AG468200 Rh7BG430200
rosa_wichuraiana Rw2G041820 Rw2G041830 Rw2G041840 Rw4G024930 Rw6G016470 Rw6G016720 Rw6G016730 Rw6G024020 Rw6G024030 Rw6G035790 Rw7G038130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 47
Acc36I ACCTGC 1 cut(s) 47
Acc65I GGTACC 1 cut(s) 831
AccB1I GGYRCC 2 cut(s) 831, 1307
AccI GTMKAC 2 cut(s) 352, 1342
AciI CCGC 4 cut(s) 241, 721, 787, 911
AclI AACGTT 1 cut(s) 229
AclWI GGATC 3 cut(s) 564, 830, 1069
AcsI RAATTY 2 cut(s) 1096, 1143
AcuI CTGAAG 2 cut(s) 351, 1011
AcyI GRCGYC 1 cut(s) 355
AfaI GTAC 4 cut(s) 577, 833, 993, 1302
AfeI AGCGCT 1 cut(s) 533
AgsI TTSAA 6 cut(s) 613, 676, 797, 1094, 1211, 1276
AjiI CACGTC 1 cut(s) 1346
AjnI CCWGG 1 cut(s) 827
AluBI AGCT 3 cut(s) 394, 647, 1010
AluI AGCT 3 cut(s) 394, 647, 1010
Alw21I GWGCWC 4 cut(s) 306, 744, 1136, 1467
Alw26I GTCTC 3 cut(s) 110, 493, 1323
AlwI GGATC 3 cut(s) 564, 830, 1069
Aor51HI AGCGCT 1 cut(s) 533
AoxI GGCC 2 cut(s) 936, 1022
ApeKI GCWGC 1 cut(s) 644
ApoI RAATTY 2 cut(s) 1096, 1143
ArsI GACNNNNNNTTYG 2 cut(s) 553, 585
Asp718I GGTACC 1 cut(s) 831
AspLEI GCGC 1 cut(s) 534
AspS9I GGNCC 1 cut(s) 936
AsuHPI GGTGA 3 cut(s) 587, 817, 1246
BaeGI GKGCMC 1 cut(s) 1312
BanI GGYRCC 2 cut(s) 831, 1307
BbsI GAAGAC 1 cut(s) 357
Bbv12I GWGCWC 4 cut(s) 306, 744, 1136, 1467
BbvI GCAGC 1 cut(s) 656
BccI CCATC 5 cut(s) 554, 606, 620, 873, 1255
BceAI ACGGC 1 cut(s) 945
BcgI CGANNNNNNTGC 2 cut(s) 731, 765
BciT130I CCWGG 1 cut(s) 829
BclI TGATCA 1 cut(s) 1389
BcoDI GTCTC 3 cut(s) 110, 493, 1323
BfaI CTAG 2 cut(s) 929, 1056
BfoI RGCGCY 1 cut(s) 535
BfuAI ACCTGC 1 cut(s) 47
BisI GCNGC 3 cut(s) 241, 645, 722
BlpI GCTNAGC 1 cut(s) 300
BlsI GCNGC 3 cut(s) 242, 646, 723
BmcAI AGTACT 1 cut(s) 577
Bme1390I CCNGG 1 cut(s) 829
BmgBI CACGTC 1 cut(s) 1346
BmgT120I GGNCC 1 cut(s) 936
BmiI GGNNCC 4 cut(s) 408, 833, 937, 1309
BmrFI CCNGG 1 cut(s) 829
BmsI GCATC 5 cut(s) 136, 177, 258, 774, 1164
BpiI GAAGAC 1 cut(s) 357
BpmI CTGGAG 1 cut(s) 1347
Bpu10I CCTNAGC 1 cut(s) 969
Bpu1102I GCTNAGC 1 cut(s) 300
BpuEI CTTGAG 1 cut(s) 380
BsaHI GRCGYC 1 cut(s) 355
BsaI GGTCTC 1 cut(s) 110
BsaJI CCNNGG 1 cut(s) 495
Bse3DI GCAATG 1 cut(s) 711
BseBI CCWGG 1 cut(s) 829
BseDI CCNNGG 1 cut(s) 495
BseGI GGATG 6 cut(s) 249, 546, 666, 884, 979, 1493
BseMI GCAATG 1 cut(s) 711
BseMII CTCAG 3 cut(s) 31, 291, 983
BseRI GAGGAG 1 cut(s) 1357
BseSI GKGCMC 1 cut(s) 1312
BseXI GCAGC 1 cut(s) 656
BseYI CCCAGC 1 cut(s) 534
BsgI GTGCAG 1 cut(s) 642
BshFI GGCC 2 cut(s) 938, 1024
BshNI GGYRCC 2 cut(s) 831, 1307
BsiHKAI GWGCWC 4 cut(s) 306, 744, 1136, 1467
BsmAI GTCTC 3 cut(s) 110, 493, 1323
BsmBI CGTCTC 1 cut(s) 1323
BsmI GAATGC 1 cut(s) 668
BsnI GGCC 2 cut(s) 938, 1024
Bso31I GGTCTC 1 cut(s) 110
Bsp1286I GDGCHC 5 cut(s) 306, 744, 1136, 1312, 1467
Bsp143I GATC 5 cut(s) 477, 556, 822, 1074, 1389
Bsp1720I GCTNAGC 1 cut(s) 300
Bsp19I CCATGG 1 cut(s) 495
BspACI CCGC 4 cut(s) 241, 721, 787, 911
BspANI GGCC 2 cut(s) 938, 1024
BspCNI CTCAG 3 cut(s) 30, 292, 982
BspHI TCATGA 1 cut(s) 601
BspLI GGNNCC 4 cut(s) 408, 833, 937, 1309
BspMI ACCTGC 1 cut(s) 47
BspPI GGATC 3 cut(s) 564, 830, 1069
BspQI GCTCTTC 1 cut(s) 634
BspT107I GGYRCC 2 cut(s) 831, 1307
BspTNI GGTCTC 1 cut(s) 110
BsrDI GCAATG 1 cut(s) 711
BssECI CCNNGG 1 cut(s) 495
BssMI GATC 5 cut(s) 477, 556, 822, 1074, 1389
BssNI GRCGYC 1 cut(s) 355
BssT1I CCWWGG 1 cut(s) 495
Bst2UI CCWGG 1 cut(s) 829
Bst4CI ACNGT 4 cut(s) 220, 389, 1300, 1414
Bst6I CTCTTC 1 cut(s) 634
BstACI GRCGYC 1 cut(s) 355
BstC8I GCNNGC 1 cut(s) 1358
BstDEI CTNAG 5 cut(s) 17, 300, 699, 969, 1500
BstDSI CCRYGG 1 cut(s) 495
BstF5I GGATG 6 cut(s) 249, 546, 666, 884, 979, 1493
BstH2I RGCGCY 1 cut(s) 535
BstHHI GCGC 1 cut(s) 534
BstKTI GATC 5 cut(s) 480, 559, 825, 1077, 1392
BstMAI GTCTC 3 cut(s) 110, 493, 1323
BstMBI GATC 5 cut(s) 477, 556, 822, 1074, 1389
BstMWI GCNNNNNNNGC 4 cut(s) 246, 263, 1227, 1331
BstNI CCWGG 1 cut(s) 829
BstNSI RCATGY 2 cut(s) 998, 1273
BstSCI CCNGG 1 cut(s) 827
BstSLI GKGCMC 1 cut(s) 1312
BstV1I GCAGC 1 cut(s) 656
BstV2I GAAGAC 1 cut(s) 357
BstXI CCANNNNNNTGG 1 cut(s) 116
BsuRI GGCC 2 cut(s) 938, 1024
BtgI CCRYGG 1 cut(s) 495
BtgZI GCGATG 1 cut(s) 1165
BtrI CACGTC 1 cut(s) 1346
BtsCI GGATG 6 cut(s) 249, 546, 666, 884, 979, 1493
BtsI GCAGTG 2 cut(s) 716, 742
BtsIMutI CAGTG 3 cut(s) 385, 716, 742
BveI ACCTGC 1 cut(s) 47
Cac8I GCNNGC 1 cut(s) 1358
CciI TCATGA 1 cut(s) 601
CfoI GCGC 1 cut(s) 534
Cfr13I GGNCC 1 cut(s) 936
CseI GACGC 2 cut(s) 363, 1340
CsiI ACCWGGT 1 cut(s) 827
Csp6I GTAC 4 cut(s) 576, 832, 992, 1301
CviQI GTAC 4 cut(s) 576, 832, 992, 1301
DdeI CTNAG 5 cut(s) 17, 300, 699, 969, 1500
DpnI GATC 5 cut(s) 479, 558, 824, 1076, 1391
DpnII GATC 5 cut(s) 477, 556, 822, 1074, 1389
Eam1104I CTCTTC 1 cut(s) 634
EarI CTCTTC 1 cut(s) 634
Eco130I CCWWGG 1 cut(s) 495
Eco147I AGGCCT 1 cut(s) 1024
Eco31I GGTCTC 1 cut(s) 110
Eco47III AGCGCT 1 cut(s) 533
Eco57I CTGAAG 2 cut(s) 351, 1011
EcoRI GAATTC 1 cut(s) 1143
EcoRII CCWGG 1 cut(s) 827
EcoT14I CCWWGG 1 cut(s) 495
EcoT22I ATGCAT 2 cut(s) 251, 1157
ErhI CCWWGG 1 cut(s) 495
Esp3I CGTCTC 1 cut(s) 1323
FauNDI CATATG 2 cut(s) 245, 273
FbaI TGATCA 1 cut(s) 1389
FblI GTMKAC 2 cut(s) 352, 1342
Fnu4HI GCNGC 3 cut(s) 241, 645, 722
FokI GGATG 6 cut(s) 236, 533, 673, 891, 986, 1480
Fsp4HI GCNGC 3 cut(s) 241, 645, 722
FspBI CTAG 2 cut(s) 929, 1056
GlaI GCGC 1 cut(s) 533
GluI GCNGC 3 cut(s) 241, 645, 722
GsaI CCCAGC 1 cut(s) 538
GsuI CTGGAG 1 cut(s) 1347
HaeII RGCGCY 1 cut(s) 535
HaeIII GGCC 2 cut(s) 938, 1024
HgaI GACGC 2 cut(s) 363, 1340
HhaI GCGC 1 cut(s) 534
Hin1I GRCGYC 1 cut(s) 355
Hin6I GCGC 1 cut(s) 532
HinP1I GCGC 1 cut(s) 532
HincII GTYRAC 2 cut(s) 227, 353
HindII GTYRAC 2 cut(s) 227, 353
HinfI GANTC 3 cut(s) 542, 916, 1004
HphI GGTGA 3 cut(s) 587, 817, 1246
Hpy166II GTNNAC 5 cut(s) 36, 227, 353, 874, 1343
Hpy188I TCNGA 3 cut(s) 370, 706, 1030
Hpy188III TCNNGA 8 cut(s) 550, 602, 610, 758, 971, 1105, 1123, 1276
Hpy8I GTNNAC 5 cut(s) 36, 227, 353, 874, 1343
Hpy99I CGWCG 1 cut(s) 357
HpyAV CCTTC 8 cut(s) 218, 368, 397, 419, 547, 1035, 1273, 1497
HpyCH4III ACNGT 4 cut(s) 220, 389, 1300, 1414
HpyCH4IV ACGT 4 cut(s) 229, 585, 619, 1345
HpyCH4V TGCA 9 cut(s) 133, 149, 249, 623, 716, 1155, 1221, 1381, 1424
HpyF10VI GCNNNNNNNGC 4 cut(s) 246, 263, 1227, 1331
HpyF3I CTNAG 5 cut(s) 17, 300, 699, 969, 1500
HpySE526I ACGT 4 cut(s) 229, 585, 619, 1345
Hsp92I GRCGYC 1 cut(s) 355
HspAI GCGC 1 cut(s) 532
KpnI GGTACC 1 cut(s) 835
Ksp22I TGATCA 1 cut(s) 1389
Kzo9I GATC 5 cut(s) 477, 556, 822, 1074, 1389
LguI GCTCTTC 1 cut(s) 634
LmnI GCTCC 3 cut(s) 739, 1131, 1470
Lsp1109I GCAGC 1 cut(s) 656
LweI GCATC 5 cut(s) 136, 177, 258, 774, 1164
MabI ACCWGGT 1 cut(s) 827
MaeI CTAG 2 cut(s) 929, 1056
MaeII ACGT 4 cut(s) 229, 585, 619, 1345
MaeIII GTNAC 4 cut(s) 694, 1223, 1252, 1346
MalI GATC 5 cut(s) 479, 558, 824, 1076, 1391
MboI GATC 5 cut(s) 477, 556, 822, 1074, 1389
MhlI GDGCHC 5 cut(s) 306, 744, 1136, 1312, 1467
MluCI AATT 9 cut(s) 182, 680, 798, 941, 1059, 1069, 1096, 1143, 1335
MmeI TCCRAC 2 cut(s) 65, 638
MnlI CCTC 9 cut(s) 282, 500, 532, 759, 1014, 1322, 1375, 1378, 1414
Mph1103I ATGCAT 2 cut(s) 251, 1157
MseI TTAA 3 cut(s) 330, 978, 1047
MslI CAYNNNNRTG 3 cut(s) 600, 999, 1259
MspA1I CMGCKG 1 cut(s) 913
MspR9I CCNGG 1 cut(s) 829
Mva1269I GAATGC 1 cut(s) 668
MvaI CCWGG 1 cut(s) 829
MwoI GCNNNNNNNGC 4 cut(s) 246, 263, 1227, 1331
NcoI CCATGG 1 cut(s) 495
NdeI CATATG 2 cut(s) 245, 273
NdeII GATC 5 cut(s) 477, 556, 822, 1074, 1389
NlaIV GGNNCC 4 cut(s) 408, 833, 937, 1309
NmeAIII GCCGAG 1 cut(s) 121
NmuCI GTSAC 3 cut(s) 694, 1252, 1346
NsiI ATGCAT 2 cut(s) 251, 1157
NspI RCATGY 2 cut(s) 998, 1273
PagI TCATGA 1 cut(s) 601
PaqCI CACCTGC 1 cut(s) 47
PceI AGGCCT 1 cut(s) 1024
PciSI GCTCTTC 1 cut(s) 634
PctI GAATGC 1 cut(s) 668
PfeI GAWTC 3 cut(s) 542, 916, 1004
PkrI GCNGC 3 cut(s) 242, 646, 723
Psp1406I AACGTT 1 cut(s) 229
Psp6I CCWGG 1 cut(s) 827
PspFI CCCAGC 1 cut(s) 534
PspGI CCWGG 1 cut(s) 827
PspN4I GGNNCC 4 cut(s) 408, 833, 937, 1309
PspPI GGNCC 1 cut(s) 936
RsaI GTAC 4 cut(s) 577, 833, 993, 1302
RsaNI GTAC 4 cut(s) 576, 832, 992, 1301
RseI CAYNNNNRTG 3 cut(s) 600, 999, 1259
SalI GTCGAC 1 cut(s) 351
SapI GCTCTTC 1 cut(s) 634
SaqAI TTAA 3 cut(s) 330, 978, 1047
SatI GCNGC 3 cut(s) 241, 645, 722
Sau3AI GATC 5 cut(s) 477, 556, 822, 1074, 1389
Sau96I GGNCC 1 cut(s) 936
ScaI AGTACT 1 cut(s) 577
ScrFI CCNGG 1 cut(s) 829
SduI GDGCHC 5 cut(s) 306, 744, 1136, 1312, 1467
SexAI ACCWGGT 1 cut(s) 827
SfaNI GCATC 5 cut(s) 136, 177, 258, 774, 1164
SmiMI CAYNNNNRTG 3 cut(s) 600, 999, 1259
SmlI CTYRAG 1 cut(s) 395
SmoI CTYRAG 1 cut(s) 395
Sse9I AATT 9 cut(s) 182, 680, 798, 941, 1059, 1069, 1096, 1143, 1335
SseBI AGGCCT 1 cut(s) 1024
SsiI CCGC 4 cut(s) 241, 721, 787, 911
SspMI CTAG 2 cut(s) 929, 1056
StuI AGGCCT 1 cut(s) 1024
StyD4I CCNGG 1 cut(s) 827
StyI CCWWGG 1 cut(s) 495
TaaI ACNGT 4 cut(s) 220, 389, 1300, 1414
TaiI ACGT 4 cut(s) 232, 588, 622, 1348
TaqI TCGA 7 cut(s) 352, 559, 751, 1104, 1290, 1314, 1416
TasI AATT 9 cut(s) 182, 680, 798, 941, 1059, 1069, 1096, 1143, 1335
TatI WGTACW 2 cut(s) 575, 991
TauI GCSGC 2 cut(s) 243, 724
TfiI GAWTC 3 cut(s) 542, 916, 1004
Tru1I TTAA 3 cut(s) 330, 978, 1047
Tru9I TTAA 3 cut(s) 330, 978, 1047
TscAI CASTG 3 cut(s) 392, 723, 749
TseFI GTSAC 3 cut(s) 694, 1252, 1346
TseI GCWGC 1 cut(s) 644
Tsp45I GTSAC 3 cut(s) 694, 1252, 1346
TspGWI ACGGA 1 cut(s) 891
TspRI CASTG 3 cut(s) 392, 723, 749
XapI RAATTY 2 cut(s) 1096, 1143
XceI RCATGY 2 cut(s) 998, 1273
XmiI GTMKAC 2 cut(s) 352, 1342
XspI CTAG 2 cut(s) 929, 1056
ZrmI AGTACT 1 cut(s) 577
Zsp2I ATGCAT 2 cut(s) 251, 1157
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.