Rh2CG494600

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0000320
Physical Location & Seq
Reverse (-)
66387235 .. 66389801
2567 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG494600.1

Sequence Viewer

Length: 1812 bp
ATGAAATCCCTGAAGAGAACTACTGGTCCAGTGATGTCGACAGAGTTTCCAGTGTTCGCTGTACTTATTATCATCCTACAGATCTTCATCTCTCTGGCCCGAGCCGAAGTGCATTACTACGATTTTATAGTTAAGGAGGTAAACTTCACAAGATTGTGCGAGTCAAAGCTCATGTTAGTAGTAAACGAGAGTTTTCCGGGTCCAGAATTACGAGCTCGCAAAGGGGATACAATTTATGTTAATGTTTACAATCAAGGATTTTATGGTTTCACCATTCACTGGCATGGAGTAAAGCAACCAAGAAATCCATGGTTTGATGGCCCGGAATATGTGACACAATGCCCAATTTCACCGGGAACAAATTTTACCTATCAAGTTCAATTGACCACAGAAGAAGGAACTGTATGGTGGCATGCTCATAGTGACTGGACGAGAGCTAGCGTTCACGGTACCATTGTCGTCTTGCCTGCTCTTGGAACAACATTTCCATTTCCTGAGCCCGATGAAGAGGAGAGCATTGTGTTTGGATCTTGGTATTTGGGAAATTTGAAACAAACGATTGACGAGTGTATGGTGCCAGAAAATGCTACCAACTTACCCGAAGCAGATGCTTACACAATAAATGGCCAACCTGGGGATTTTGCTCTATGCTCCAAGAATTCAACATATCGTCTTAAGGTGGACTATGGCAAGACGTATCTTCTTCGGATACTGAACGCCAACATTGATGCAGAGATGTACTTTGCTATTGCTGAGCACAATCTCACTATGGTTGGCATGGACGGTTCCTATACCAAACCCATCAACACAAGCTACATAATGATAAGCCCTGGACAAACAATGGATGTATTGCTCAACACAAATTCGACTCTGGGCAGCTATTACATGGTCGCAAGAGAATACGTGAGTGCTACAGTTAACTCAGTATCTAGCTATCTGGATGATGCTGTTGCAATCCTTGAATATAATGGCAACTATACCACTTCGGAAGCTCCGATATATCCTGAAAACCTTCCCATGTCCTTATCCCAAGAACCAGCATACGACTTCCTCAGGAAAATTAGAAGCTTAGCCACCCCAGAATATCCTATAAATGTCCCAAAAGAGAGTGACGTTACTAAGAGAATGTATATAACAGCTTCCTCCAACGCTCTTTACTGTACTCCAGATTTAGGTCCCACATGCGTCAACATATCTTGGGCTGCAAGCGTCAATAATATTAGTTGGGTTAATCCATCAGAATCTATCTTGCAAGCCTACTACAACAACATAAGCGAACCAGTTTATGAAACAGACTTCCCAGATGAGCCGCCTGTTTATTTTAACTTCACTGATGATTCATCAGCAATAGATGTAGGCTTAACAGTCCAAGGGACAAAGGTGACGATGCTGGAATACAATGAAACAGTTGAGATGGTGTTTCAAGGAACTGATGTGGAGGGAGCGGCCGTGAATCATCCAATGCACGTGCATGGACTTAGCTTTTATGTGCTTGGTTTTGGTTTTGGGAATTACTTCGCCGAGAGGGACTCAAAAACCTTCAATTTGATTGATCCTCCTTATGTAGCAACGTTCATAACTCCAAAAAATGGATGGGTAGCTCTCAGATTCGTAGCGAATAATCCAGGTGTATGGTTTTGGCATTGTCATATGGAGAGACACTTGACATGGGGTATGGAGTCTGCTTTTATAGTGAAGAACGGGGGCACACCTGAGACTACTATGCTCCCTCCTCCGGCTACAATGCCTTCCTGTGACGTTCCATTGAAGTCTTCAGCCATCCTAGAGAAGCAACTAGAACGGACTAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00053 ko01100 map00053 map01100
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

603

Amino Acids

67.73

Weight (kDa)

4.81

Isoelectric Point (pI)

43.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_3 PF07732 44 - 156 1.1e-37 Multicopper oxidase
Cu-oxidase PF00394 172 - 325 9.3e-33 Multicopper oxidase
Cu-oxidase_2 PF07731 433 - 567 4.9e-35 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000320)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g12640 FvH4_2g18980 FvH4_2g18990 FvH4_2g40270 FvH4_2g40270 FvH4_4g22740 FvH4_4g22740 FvH4_5g35760 FvH4_5g35760 FvH4_5g35760 FvH4_5g35760 FvH4_5g35760 FvH4_5g35760 FvH4_5g35760 FvH4_5g35760 FvH4_5g36460 FvH4_5g36460 FvH4_6g39950 FvH4_6g39970 FvH4_6g39983 FvH4_6g39990
rosa_chinensis RchiOBHm_Chr2g0154481 RchiOBHm_Chr2g0154511 RchiOBHm_Chr4g0428961 RchiOBHm_Chr6g0252431 RchiOBHm_Chr6g0273301 RchiOBHm_Chr6g0273631 RchiOBHm_Chr6g0273651 RchiOBHm_Chr6g0284751 RchiOBHm_Chr6g0284761 RchiOBHm_Chr6g0300491 RchiOBHm_Chr7g0237421 RchiOBHm_Chr7g0238581
rosa_laevigata RLG00000000906 RLG00000001006 RLG00000007110 RLG00000011333 RLG00000012721 RLG00000013605 RLG00000013626 RLG00000020780 RLG00000020782 RLG00000020783 RLG00000020786
rosa_multiflora Rmu_co8321753.1_g000001 Rmu_sc0000239.1_g000020 Rmu_sc0000686.1_g000001 Rmu_sc0000686.1_g000003 Rmu_sc0000686.1_g000005 Rmu_sc0001476.1_g000011 Rmu_sc0002231.1_g000002 Rmu_sc0002231.1_g000016 Rmu_sc0002690.1_g000003 Rmu_sc0002717.1_g000015 Rmu_sc0002923.1_g000026 Rmu_sc0003808.1_g000016 Rmu_sc0006475.1_g000008 Rmu_sc0006475.1_g000012 Rmu_sc0006475.1_g000018 Rmu_sc0014815.1_g000004 Rmu_sc0015313.1_g000012
rosa_roxburghii Rroxscaffold_2G00094270 Rroxscaffold_2G00094290 Rroxscaffold_3G00222950 Rroxscaffold_3G00224250 Rroxscaffold_5G00370630 Rroxscaffold_7G00167740 Rroxscaffold_7G00184410 Rroxscaffold_7G00195190 Rroxscaffold_7G00195460
rosa_rugosa Rorug02G0444600 Rorug04G0231100 Rorug04G0231200 Rorug06G0074000 Rorug06G0076800 Rorug06G0166400 Rorug06G0166500 Rorug06G0166600 Rorug06G0296400 Rorug07G0303000
rosa_samantha Rh2CG494600 Rh2CG494700 Rh2DG530800 Rh2DG530900 Rh2DG531000 Rh4AG287500 Rh4AG403800 Rh4BG293400 Rh4BG415100 Rh4DG290500 Rh6AG191000 Rh6AG191300 Rh6BG192500 Rh6BG194800 Rh6BG195200 Rh6BG279500 Rh6BG279600 Rh6DG183900 Rh6DG186600 Rh6DG274300 Rh6DG274500 Rh6DG409500 Rh7AG460600 Rh7AG468200 Rh7BG430200
rosa_wichuraiana Rw2G041820 Rw2G041830 Rw2G041840 Rw4G024930 Rw6G016470 Rw6G016720 Rw6G016730 Rw6G024020 Rw6G024030 Rw6G035790 Rw7G038130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 449
AccB1I GGYRCC 2 cut(s) 449, 574
AccB7I CCANNNNNTGG 2 cut(s) 279, 1589
AccBSI CCGCTC 1 cut(s) 1445
AccI GTMKAC 1 cut(s) 38
AciI CCGC 2 cut(s) 1310, 1445
AclI AACGTT 1 cut(s) 1571
AclWI GGATC 2 cut(s) 535, 1547
AcoI YGGCCR 2 cut(s) 625, 1446
AcsI RAATTY 4 cut(s) 361, 544, 658, 862
AcuI CTGAAG 2 cut(s) 32, 1758
AcvI CACGTG 1 cut(s) 1468
AfaI GTAC 4 cut(s) 63, 451, 740, 1162
AfiI CCNNNNNNNGG 6 cut(s) 279, 473, 634, 1172, 1589, 1735
AflII CTTAAG 1 cut(s) 674
AgsI TTSAA 7 cut(s) 380, 550, 663, 962, 1424, 1543, 1768
AjnI CCWGG 3 cut(s) 631, 829, 1624
AloI GAACNNNNNNTCC 4 cut(s) 10, 42, 469, 501
Alw21I GWGCWC 2 cut(s) 217, 759
Alw26I GTCTC 2 cut(s) 1651, 1709
AlwI GGATC 2 cut(s) 535, 1547
Ama87I CYCGRG 1 cut(s) 99
AoxI GGCC 4 cut(s) 96, 319, 625, 1446
ApeKI GCWGC 2 cut(s) 876, 1202
ApoI RAATTY 4 cut(s) 361, 544, 658, 862
Asp700I GAANNNNTTC 2 cut(s) 1011, 1514
Asp718I GGTACC 1 cut(s) 449
AspS9I GGNCC 5 cut(s) 26, 97, 200, 320, 1175
AsuC2I CCSGG 3 cut(s) 198, 323, 354
AsuHPI GGTGA 3 cut(s) 262, 342, 1393
AsuNHI GCTAGC 1 cut(s) 437
AvaI CYCGRG 1 cut(s) 99
AvaII GGWCC 3 cut(s) 26, 200, 1175
AxyI CCTNAGG 1 cut(s) 1052
BaeGI GKGCMC 1 cut(s) 1709
BaeI ACNNNNGTAYC 2 cut(s) 441, 474
BalI TGGCCA 1 cut(s) 627
BanI GGYRCC 2 cut(s) 449, 574
BanII GRGCYC 2 cut(s) 217, 501
BbrPI CACGTG 1 cut(s) 1468
BbsI GAAGAC 1 cut(s) 1764
Bbv12I GWGCWC 2 cut(s) 217, 759
BbvI GCAGC 2 cut(s) 888, 1189
BccI CCATC 6 cut(s) 311, 809, 1243, 1408, 1587, 1787
BceAI ACGGC 1 cut(s) 1433
BcgI CGANNNNNNTGC 2 cut(s) 590, 624
BciT130I CCWGG 3 cut(s) 633, 831, 1626
BciVI GTATCC 2 cut(s) 220, 702
BcnI CCSGG 3 cut(s) 198, 323, 354
BcoDI GTCTC 2 cut(s) 1651, 1709
BfaI CTAG 4 cut(s) 438, 930, 1784, 1796
BfmI CTRYAG 2 cut(s) 77, 912
BfrI CTTAAG 1 cut(s) 674
BfuI GTATCC 2 cut(s) 220, 702
BglII AGATCT 1 cut(s) 81
BisI GCNGC 4 cut(s) 877, 1203, 1310, 1446
BlpI GCTNAGC 2 cut(s) 753, 1069
BlsI GCNGC 4 cut(s) 878, 1204, 1311, 1447
Bme1390I CCNGG 6 cut(s) 198, 323, 354, 633, 831, 1626
Bme18I GGWCC 3 cut(s) 26, 200, 1175
BmeT110I CYCGRG 1 cut(s) 99
BmgT120I GGNCC 5 cut(s) 26, 97, 200, 320, 1175
BmiI GGNNCC 5 cut(s) 201, 451, 576, 787, 1177
BmrFI CCNGG 6 cut(s) 198, 323, 354, 633, 831, 1626
BmsI GCATC 4 cut(s) 598, 718, 934, 1377
BmtI GCTAGC 1 cut(s) 441
BpiI GAAGAC 1 cut(s) 1764
BplI GAGNNNNNCTC 2 cut(s) 1514, 1546
BpmI CTGGAG 1 cut(s) 1149
Bpu10I CCTNAGC 1 cut(s) 495
Bpu1102I GCTNAGC 2 cut(s) 753, 1069
BpuMI CCSGG 3 cut(s) 198, 323, 354
BsaAI YACGTR 2 cut(s) 904, 1468
BsaBI GATNNNNATC 1 cut(s) 86
BsaJI CCNNGG 4 cut(s) 308, 632, 829, 1369
BsaXI ACNNNNNCTCC 2 cut(s) 1434, 1464
Bsc4I CCNNNNNNNGG 6 cut(s) 279, 473, 634, 1172, 1589, 1735
Bse1I ACTGG 6 cut(s) 28, 29, 50, 284, 431, 1280
Bse21I CCTNAGG 1 cut(s) 1052
Bse8I GATNNNNATC 1 cut(s) 86
BseBI CCWGG 3 cut(s) 633, 831, 1626
BseDI CCNNGG 4 cut(s) 308, 632, 829, 1369
BseGI GGATG 6 cut(s) 72, 850, 946, 1456, 1598, 1779
BseJI GATNNNNATC 1 cut(s) 86
BseLI CCNNNNNNNGG 6 cut(s) 279, 473, 634, 1172, 1589, 1735
BseMII CTCAG 6 cut(s) 486, 744, 936, 1066, 1618, 1704
BseNI ACTGG 6 cut(s) 28, 29, 50, 284, 431, 1280
BseRI GAGGAG 2 cut(s) 524, 1722
BseSI GKGCMC 1 cut(s) 1709
BseX3I CGGCCG 1 cut(s) 1446
BseXI GCAGC 2 cut(s) 888, 1189
Bsh1285I CGRYCG 1 cut(s) 1449
BshFI GGCC 4 cut(s) 98, 321, 627, 1448
BshNI GGYRCC 2 cut(s) 449, 574
BsiEI CGRYCG 1 cut(s) 1449
BsiHKAI GWGCWC 2 cut(s) 217, 759
BsiHKCI CYCGRG 1 cut(s) 99
BsiSI CCGG 4 cut(s) 197, 323, 353, 1736
BslFI GGGAC 4 cut(s) 1082, 1161, 1387, 1541
BslI CCNNNNNNNGG 6 cut(s) 279, 473, 634, 1172, 1589, 1735
BsmAI GTCTC 2 cut(s) 1651, 1709
BsmFI GGGAC 4 cut(s) 1082, 1161, 1387, 1541
BsnI GGCC 4 cut(s) 98, 321, 627, 1448
BsoBI CYCGRG 1 cut(s) 99
Bsp1286I GDGCHC 4 cut(s) 217, 501, 759, 1709
Bsp143I GATC 3 cut(s) 81, 527, 1552
Bsp1720I GCTNAGC 2 cut(s) 753, 1069
Bsp19I CCATGG 1 cut(s) 308
BspACI CCGC 2 cut(s) 1310, 1445
BspANI GGCC 4 cut(s) 98, 321, 627, 1448
BspCNI CTCAG 6 cut(s) 487, 745, 935, 1065, 1617, 1705
BspLI GGNNCC 5 cut(s) 201, 451, 576, 787, 1177
BspOI GCTAGC 1 cut(s) 441
BspPI GGATC 2 cut(s) 535, 1547
BspT107I GGYRCC 2 cut(s) 449, 574
BspTI CTTAAG 1 cut(s) 674
BsrBI CCGCTC 1 cut(s) 1445
BsrI ACTGG 6 cut(s) 28, 29, 50, 284, 431, 1280
BssECI CCNNGG 4 cut(s) 308, 632, 829, 1369
BssMI GATC 3 cut(s) 81, 527, 1552
BssT1I CCWWGG 2 cut(s) 308, 1369
Bst2UI CCWGG 3 cut(s) 633, 831, 1626
Bst4CI ACNGT 7 cut(s) 403, 449, 785, 916, 1160, 1366, 1408
Bst6I CTCTTC 2 cut(s) 8, 501
BstAFI CTTAAG 1 cut(s) 674
BstBAI YACGTR 2 cut(s) 904, 1468
BstC8I GCNNGC 6 cut(s) 217, 414, 439, 468, 1207, 1254
BstDEI CTNAG 9 cut(s) 495, 753, 922, 1052, 1069, 1119, 1478, 1604, 1713
BstDSI CCRYGG 1 cut(s) 308
BstF5I GGATG 6 cut(s) 72, 850, 946, 1456, 1598, 1779
BstKTI GATC 3 cut(s) 84, 530, 1555
BstMAI GTCTC 2 cut(s) 1651, 1709
BstMBI GATC 3 cut(s) 81, 527, 1552
BstMCI CGRYCG 1 cut(s) 1449
BstNI CCWGG 3 cut(s) 633, 831, 1626
BstNSI RCATGY 2 cut(s) 416, 1185
BstSCI CCNGG 6 cut(s) 196, 321, 352, 631, 829, 1624
BstSFI CTRYAG 2 cut(s) 77, 912
BstSLI GKGCMC 1 cut(s) 1709
BstV1I GCAGC 2 cut(s) 888, 1189
BstV2I GAAGAC 1 cut(s) 1764
BstX2I RGATCY 2 cut(s) 81, 527
BstXI CCANNNNNNTGG 1 cut(s) 1632
BstYI RGATCY 2 cut(s) 81, 527
BstZI CGGCCG 1 cut(s) 1446
Bsu36I CCTNAGG 1 cut(s) 1052
BsuI GTATCC 2 cut(s) 220, 702
BsuRI GGCC 4 cut(s) 98, 321, 627, 1448
BtgI CCRYGG 1 cut(s) 308
BtsCI GGATG 6 cut(s) 72, 850, 946, 1456, 1598, 1779
BtsIMutI CAGTG 4 cut(s) 36, 57, 277, 1329
Cac8I GCNNGC 6 cut(s) 217, 414, 439, 468, 1207, 1254
Cfr13I GGNCC 5 cut(s) 26, 97, 200, 320, 1175
CseI GACGC 2 cut(s) 1174, 1198
Csp6I GTAC 4 cut(s) 62, 450, 739, 1161
CviQI GTAC 4 cut(s) 62, 450, 739, 1161
DdeI CTNAG 9 cut(s) 495, 753, 922, 1052, 1069, 1119, 1478, 1604, 1713
DpnI GATC 3 cut(s) 83, 529, 1554
DpnII GATC 3 cut(s) 81, 527, 1552
EaeI YGGCCR 2 cut(s) 625, 1446
EagI CGGCCG 1 cut(s) 1446
Eam1104I CTCTTC 2 cut(s) 8, 501
EarI CTCTTC 2 cut(s) 8, 501
Ecl136II GAGCTC 1 cut(s) 215
EclXI CGGCCG 1 cut(s) 1446
Eco130I CCWWGG 2 cut(s) 308, 1369
Eco24I GRGCYC 2 cut(s) 217, 501
Eco47I GGWCC 3 cut(s) 26, 200, 1175
Eco52I CGGCCG 1 cut(s) 1446
Eco53kI GAGCTC 1 cut(s) 215
Eco57I CTGAAG 2 cut(s) 32, 1758
Eco72I CACGTG 1 cut(s) 1468
Eco81I CCTNAGG 1 cut(s) 1052
Eco88I CYCGRG 1 cut(s) 99
EcoICRI GAGCTC 1 cut(s) 215
EcoO109I RGGNCCY 1 cut(s) 1175
EcoRI GAATTC 1 cut(s) 658
EcoRII CCWGG 3 cut(s) 631, 829, 1624
EcoT14I CCWWGG 2 cut(s) 308, 1369
EcoT38I GRGCYC 2 cut(s) 217, 501
ErhI CCWWGG 2 cut(s) 308, 1369
FaqI GGGAC 4 cut(s) 1082, 1161, 1387, 1541
FauNDI CATATG 1 cut(s) 1650
FblI GTMKAC 1 cut(s) 38
Fnu4HI GCNGC 4 cut(s) 877, 1203, 1310, 1446
FokI GGATG 6 cut(s) 59, 857, 953, 1443, 1605, 1766
FriOI GRGCYC 2 cut(s) 217, 501
Fsp4HI GCNGC 4 cut(s) 877, 1203, 1310, 1446
FspBI CTAG 4 cut(s) 438, 930, 1784, 1796
GluI GCNGC 4 cut(s) 877, 1203, 1310, 1446
GsuI CTGGAG 1 cut(s) 1149
HaeIII GGCC 4 cut(s) 98, 321, 627, 1448
HapII CCGG 4 cut(s) 197, 323, 353, 1736
HgaI GACGC 2 cut(s) 1174, 1198
HincII GTYRAC 3 cut(s) 39, 919, 1189
HindII GTYRAC 3 cut(s) 39, 919, 1189
HindIII AAGCTT 1 cut(s) 1066
HinfI GANTC 8 cut(s) 161, 868, 1241, 1337, 1453, 1529, 1608, 1679
HpaI GTTAAC 1 cut(s) 919
HpaII CCGG 4 cut(s) 197, 323, 353, 1736
HphI GGTGA 3 cut(s) 262, 342, 1393
Hpy166II GTNNAC 8 cut(s) 39, 142, 184, 247, 445, 682, 919, 1189
Hpy188I TCNGA 5 cut(s) 708, 988, 996, 1240, 1607
Hpy188III TCNNGA 6 cut(s) 203, 494, 938, 1004, 1054, 1166
Hpy8I GTNNAC 8 cut(s) 39, 142, 184, 247, 445, 682, 919, 1189
HpyAV CCTTC 4 cut(s) 389, 1022, 1549, 1758
HpyCH4III ACNGT 7 cut(s) 403, 449, 785, 916, 1160, 1366, 1408
HpyCH4IV ACGT 6 cut(s) 695, 903, 1113, 1467, 1571, 1758
HpyCH4V TGCA 7 cut(s) 112, 731, 953, 1205, 1252, 1465, 1471
HpyF3I CTNAG 9 cut(s) 495, 753, 922, 1052, 1069, 1119, 1478, 1604, 1713
HpySE526I ACGT 6 cut(s) 695, 903, 1113, 1467, 1571, 1758
KpnI GGTACC 1 cut(s) 453
KspAI GTTAAC 1 cut(s) 919
Kzo9I GATC 3 cut(s) 81, 527, 1552
LmnI GCTCC 4 cut(s) 656, 997, 1442, 1731
Lsp1109I GCAGC 2 cut(s) 888, 1189
LweI GCATC 4 cut(s) 598, 718, 934, 1377
MaeI CTAG 4 cut(s) 438, 930, 1784, 1796
MaeII ACGT 6 cut(s) 695, 903, 1113, 1467, 1571, 1758
MaeIII GTNAC 6 cut(s) 331, 422, 1109, 1114, 1381, 1754
MalI GATC 3 cut(s) 83, 529, 1554
MbiI CCGCTC 1 cut(s) 1445
MboI GATC 3 cut(s) 81, 527, 1552
MboII GAAGA 8 cut(s) 25, 76, 404, 518, 692, 695, 1708, 1764
MfeI CAATTG 1 cut(s) 380
MflI RGATCY 2 cut(s) 81, 527
MhlI GDGCHC 4 cut(s) 217, 501, 759, 1709
MlsI TGGCCA 1 cut(s) 627
MluNI TGGCCA 1 cut(s) 627
MlyI GAGTC 4 cut(s) 170, 862, 1523, 1688
MmeI TCCRAC 1 cut(s) 1170
MnlI CCTC 9 cut(s) 130, 502, 1061, 1153, 1432, 1518, 1566, 1740, 1743
Mox20I TGGCCA 1 cut(s) 627
MroXI GAANNNNTTC 2 cut(s) 1011, 1514
MscI TGGCCA 1 cut(s) 627
MseI TTAA 8 cut(s) 132, 240, 675, 918, 1230, 1323, 1361, 1810
MslI CAYNNNNRTG 2 cut(s) 282, 1470
Msp20I TGGCCA 1 cut(s) 627
MspCI CTTAAG 1 cut(s) 674
MspI CCGG 4 cut(s) 197, 323, 353, 1736
MspR9I CCNGG 6 cut(s) 198, 323, 354, 633, 831, 1626
MunI CAATTG 1 cut(s) 380
MvaI CCWGG 3 cut(s) 633, 831, 1626
NciI CCSGG 3 cut(s) 198, 323, 354
NcoI CCATGG 1 cut(s) 308
NdeI CATATG 1 cut(s) 1650
NdeII GATC 3 cut(s) 81, 527, 1552
NheI GCTAGC 1 cut(s) 437
NlaIV GGNNCC 5 cut(s) 201, 451, 576, 787, 1177
NmeAIII GCCGAG 1 cut(s) 1546
NmuCI GTSAC 5 cut(s) 331, 422, 1109, 1381, 1754
NspI RCATGY 2 cut(s) 416, 1185
PaeI GCATGC 1 cut(s) 416
PcsI WCGNNNNNNNCGW 1 cut(s) 992
PdmI GAANNNNTTC 2 cut(s) 1011, 1514
PfeI GAWTC 4 cut(s) 1241, 1337, 1453, 1608
PflMI CCANNNNNTGG 2 cut(s) 279, 1589
PkrI GCNGC 4 cut(s) 878, 1204, 1311, 1447
PleI GAGTC 4 cut(s) 169, 862, 1523, 1687
PmaCI CACGTG 1 cut(s) 1468
PmlI CACGTG 1 cut(s) 1468
PpsI GAGTC 4 cut(s) 169, 862, 1523, 1687
Ppu21I YACGTR 2 cut(s) 904, 1468
PpuMI RGGWCCY 1 cut(s) 1175
Psp124BI GAGCTC 1 cut(s) 217
Psp1406I AACGTT 1 cut(s) 1571
Psp5II RGGWCCY 1 cut(s) 1175
Psp6I CCWGG 3 cut(s) 631, 829, 1624
PspCI CACGTG 1 cut(s) 1468
PspGI CCWGG 3 cut(s) 631, 829, 1624
PspN4I GGNNCC 5 cut(s) 201, 451, 576, 787, 1177
PspPI GGNCC 5 cut(s) 26, 97, 200, 320, 1175
PspPPI RGGWCCY 1 cut(s) 1175
PsrI GAACNNNNNNTAC 2 cut(s) 349, 381
PsuI RGATCY 2 cut(s) 81, 527
RsaI GTAC 4 cut(s) 63, 451, 740, 1162
RsaNI GTAC 4 cut(s) 62, 450, 739, 1161
RseI CAYNNNNRTG 2 cut(s) 282, 1470
SacI GAGCTC 1 cut(s) 217
SalI GTCGAC 1 cut(s) 37
SaqAI TTAA 8 cut(s) 132, 240, 675, 918, 1230, 1323, 1361, 1810
SatI GCNGC 4 cut(s) 877, 1203, 1310, 1446
Sau3AI GATC 3 cut(s) 81, 527, 1552
Sau96I GGNCC 5 cut(s) 26, 97, 200, 320, 1175
SchI GAGTC 4 cut(s) 170, 862, 1523, 1688
ScrFI CCNGG 6 cut(s) 198, 323, 354, 633, 831, 1626
SduI GDGCHC 4 cut(s) 217, 501, 759, 1709
SfaNI GCATC 4 cut(s) 598, 718, 934, 1377
SfcI CTRYAG 2 cut(s) 77, 912
SinI GGWCC 3 cut(s) 26, 200, 1175
SmiMI CAYNNNNRTG 2 cut(s) 282, 1470
SmlI CTYRAG 1 cut(s) 674
SmoI CTYRAG 1 cut(s) 674
SphI GCATGC 1 cut(s) 416
SsiI CCGC 2 cut(s) 1310, 1445
SspI AATATT 1 cut(s) 1219
SspMI CTAG 4 cut(s) 438, 930, 1784, 1796
SstI GAGCTC 1 cut(s) 217
StyD4I CCNGG 6 cut(s) 196, 321, 352, 631, 829, 1624
StyI CCWWGG 2 cut(s) 308, 1369
TaaI ACNGT 7 cut(s) 403, 449, 785, 916, 1160, 1366, 1408
TaiI ACGT 6 cut(s) 698, 906, 1116, 1470, 1574, 1761
TaqI TCGA 2 cut(s) 38, 866
TatI WGTACW 3 cut(s) 61, 738, 1160
TauI GCSGC 2 cut(s) 1312, 1448
TfiI GAWTC 4 cut(s) 1241, 1337, 1453, 1608
Tru1I TTAA 8 cut(s) 132, 240, 675, 918, 1230, 1323, 1361, 1810
Tru9I TTAA 8 cut(s) 132, 240, 675, 918, 1230, 1323, 1361, 1810
TscAI CASTG 4 cut(s) 36, 57, 284, 1336
TseFI GTSAC 5 cut(s) 331, 422, 1109, 1381, 1754
TseI GCWGC 2 cut(s) 876, 1202
Tsp45I GTSAC 5 cut(s) 331, 422, 1109, 1381, 1754
TspDTI ATGAA 7 cut(s) 17, 76, 519, 1302, 1329, 1416, 1564
TspRI CASTG 4 cut(s) 36, 57, 284, 1336
Van91I CCANNNNNTGG 2 cut(s) 279, 1589
Vha464I CTTAAG 1 cut(s) 674
VpaK11BI GGWCC 3 cut(s) 26, 200, 1175
XapI RAATTY 4 cut(s) 361, 544, 658, 862
XceI RCATGY 2 cut(s) 416, 1185
XcmI CCANNNNNNNNNTGG 2 cut(s) 306, 1590
XmiI GTMKAC 1 cut(s) 38
XmnI GAANNNNTTC 2 cut(s) 1011, 1514
XspI CTAG 4 cut(s) 438, 930, 1784, 1796
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.