RLG00000020780

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0000320
Physical Location & Seq
Reverse (-)
68655834 .. 68658346
2513 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020780

Sequence Viewer

Length: 1779 bp
ATGTCGACACAGTTTCCGGTGTTCGCTGTTCTTATTATCATCCTACAGCTCTTCATCTCTCTGGCTCGAGCCGAAGTGCATTACTACGATTTTATAGTTAAGGAGGTAAACTTCACAAGACTCTGTGAGTCAAAGCTCATGTTAGTAGTAAACGAGAGTTTTCCGGGTCCAGAATTACGAGCTCGCAAAGGGGATACAATTTATGTTAATGTTTACAACCAAGGATTTTATGGTTTCACCATTCACTGGCATGGAGTAAAGCAACCAAGAAATCCATGGTTTGATGGCCCGGAATTTGTGACACAATGCCCAATTTCACCGGGAACAAATTTTACCTATCAAGTTCAATTGACCACAGAAGAAGGAACTGTATGGTGGCATGCTCACAGTGACTGGACGAGAGCTAGCGTTCACGGTACCATCGTCGTCTTGCCTGCTCTTGGAACAACATTTCCATTTCCTGAGCCCGATGAAGAGGAGAGCATTGTGTTTGGATCTTGGTATTTGGGAAATTTGAAACAAACGATTGACGAGTGTATGGTGCCAGAAAATGCTACCAACTTACCCGAAGCAGATGCTTACACAATAAATGGCCAACCTGGGGATTTTGCTCTATGCTCCAAGAATTCAACATATCGTCTTAAGGTGGACTATGGCAAGACATATCTTCTTCGGATACTTAACGCAAACATTGATGCAGAGATGTACTTTGCAATTGCTGAGCACAATCTCACTGTGGTTGGCATGGACGGTTCCTATACCAAACCCATCAACACAAGCTACATAATGATAAGCCCCGGACAAACAATGGATGTGTTGCTCAACACAAATTCGACTCTGGGGAGCTATTATATGGTCGCAAGAGAATACGTGAGTGCTACAGTTAACTCAGTATCTAGCTATCTGGATGATGCTGTTGCAATCCTTGAATATAATGGCAACTATACCACTTCGGAAGCTCCTATATATCCTGAAAACCTTCCCATGTCCTTATCTCAAGAACCAGCATACGACTTCATCAGGAAAATTAGAAGCTTAGCCACCCCAGAATATCCTATAAATGTCCCAAAAGAGAGTGACGTTACTAAGAGAATGTATGTAACAGCTTCCTCCAACGCTCTTTACTGCACTCCAGATTTAGGTCCCACATGCGTCAACATATCTTGGGCTGCGAGCGTCAATAATATTAGTTGGGTTAATCCATCAGAACCTGTTTTGCAAGCCTACTACAACAACATAAGCGAACCGATTTATGAAACAGACTTCCCAGATGAGCCGCCTGTTTATTTTAACTTCACTGATGATTCATCAGCAATAGATGTAGGCTTAACAGTCCAAGGGACGAAGGTGACGATGCTGGAATACAATGAAACAGTTGAGATGGTGTTTCAAGGAACTGATGTGGAGGGAGCCGGCGTGAATCATCCAATGCACGTGCATGGACTTAGCTTTTATGTGCTTGGTTTTGGTTTTGGGAATTACTTCGCCGAGAGGGACTCAAAAACCTTCAATTTGATTGATCCTCCTTACGTAGCAACGTTCATAACTCCAAAAAATGGATGGGTAGCTATCAGATTCGTAGCGAATAATCCAGGTGTATGGTTTTGGCATTGTCATATGGAGAGACACTTGACATGGGGTATGGAGTCTGCTTTTATAGTGAAGAACGGGGGCACACCTGAGACTACTATGCTCCCCCCTCCGGCTACAATGCCTTCCTGTGACGTTCCATTGAAGTCTTCAGCCATCCTAGAGAAGCAACTAGAACGGACTGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00053 ko01100 map00053 map01100
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

593

Amino Acids

66.47

Weight (kDa)

4.71

Isoelectric Point (pI)

41.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_3 PF07732 33 - 145 2e-37 Multicopper oxidase
Cu-oxidase PF00394 161 - 314 9.3e-34 Multicopper oxidase
Cu-oxidase_2 PF07731 422 - 556 1.6e-35 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000320)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g12640 FvH4_2g18980 FvH4_2g18990 FvH4_2g40270 FvH4_2g40270 FvH4_4g22740 FvH4_4g22740 FvH4_5g35760 FvH4_5g35760 FvH4_5g35760 FvH4_5g35760 FvH4_5g35760 FvH4_5g35760 FvH4_5g35760 FvH4_5g35760 FvH4_5g36460 FvH4_5g36460 FvH4_6g39950 FvH4_6g39970 FvH4_6g39983 FvH4_6g39990
rosa_chinensis RchiOBHm_Chr2g0154481 RchiOBHm_Chr2g0154511 RchiOBHm_Chr4g0428961 RchiOBHm_Chr6g0252431 RchiOBHm_Chr6g0273301 RchiOBHm_Chr6g0273631 RchiOBHm_Chr6g0273651 RchiOBHm_Chr6g0284751 RchiOBHm_Chr6g0284761 RchiOBHm_Chr6g0300491 RchiOBHm_Chr7g0237421 RchiOBHm_Chr7g0238581
rosa_laevigata RLG00000000906 RLG00000001006 RLG00000007110 RLG00000011333 RLG00000012721 RLG00000013605 RLG00000013626 RLG00000020780 RLG00000020782 RLG00000020783 RLG00000020786
rosa_multiflora Rmu_co8321753.1_g000001 Rmu_sc0000239.1_g000020 Rmu_sc0000686.1_g000001 Rmu_sc0000686.1_g000003 Rmu_sc0000686.1_g000005 Rmu_sc0001476.1_g000011 Rmu_sc0002231.1_g000002 Rmu_sc0002231.1_g000016 Rmu_sc0002690.1_g000003 Rmu_sc0002717.1_g000015 Rmu_sc0002923.1_g000026 Rmu_sc0003808.1_g000016 Rmu_sc0006475.1_g000008 Rmu_sc0006475.1_g000012 Rmu_sc0006475.1_g000018 Rmu_sc0014815.1_g000004 Rmu_sc0015313.1_g000012
rosa_roxburghii Rroxscaffold_2G00094270 Rroxscaffold_2G00094290 Rroxscaffold_3G00222950 Rroxscaffold_3G00224250 Rroxscaffold_5G00370630 Rroxscaffold_7G00167740 Rroxscaffold_7G00184410 Rroxscaffold_7G00195190 Rroxscaffold_7G00195460
rosa_rugosa Rorug02G0444600 Rorug04G0231100 Rorug04G0231200 Rorug06G0074000 Rorug06G0076800 Rorug06G0166400 Rorug06G0166500 Rorug06G0166600 Rorug06G0296400 Rorug07G0303000
rosa_samantha Rh2CG494600 Rh2CG494700 Rh2DG530800 Rh2DG530900 Rh2DG531000 Rh4AG287500 Rh4AG403800 Rh4BG293400 Rh4BG415100 Rh4DG290500 Rh6AG191000 Rh6AG191300 Rh6BG192500 Rh6BG194800 Rh6BG195200 Rh6BG279500 Rh6BG279600 Rh6DG183900 Rh6DG186600 Rh6DG274300 Rh6DG274500 Rh6DG409500 Rh7AG460600 Rh7AG468200 Rh7BG430200
rosa_wichuraiana Rw2G041820 Rw2G041830 Rw2G041840 Rw4G024930 Rw6G016470 Rw6G016720 Rw6G016730 Rw6G024020 Rw6G024030 Rw6G035790 Rw7G038130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 416
AccB1I GGYRCC 2 cut(s) 416, 541
AccB7I CCANNNNNTGG 2 cut(s) 246, 1556
AccI GTMKAC 1 cut(s) 5
AciI CCGC 1 cut(s) 1277
AclI AACGTT 1 cut(s) 1538
AclWI GGATC 2 cut(s) 502, 1514
AcoI YGGCCR 1 cut(s) 592
AcsI RAATTY 5 cut(s) 293, 328, 511, 625, 829
AcuI CTGAAG 1 cut(s) 1725
AcvI CACGTG 1 cut(s) 1435
AfaI GTAC 2 cut(s) 418, 707
AfiI CCNNNNNNNGG 6 cut(s) 246, 440, 601, 1139, 1556, 1702
AflII CTTAAG 1 cut(s) 641
AgsI TTSAA 7 cut(s) 347, 517, 630, 929, 1391, 1510, 1735
AjnI CCWGG 2 cut(s) 598, 1591
AloI GAACNNNNNNTCC 2 cut(s) 436, 468
Alw21I GWGCWC 2 cut(s) 184, 726
Alw26I GTCTC 2 cut(s) 1618, 1676
AlwI GGATC 2 cut(s) 502, 1514
AlwNI CAGNNNCTG 2 cut(s) 393, 1211
Ama87I CYCGRG 1 cut(s) 66
AoxI GGCC 2 cut(s) 286, 592
ApeKI GCWGC 1 cut(s) 1169
ApoI RAATTY 5 cut(s) 293, 328, 511, 625, 829
Asp700I GAANNNNTTC 2 cut(s) 978, 1481
Asp718I GGTACC 1 cut(s) 416
AspS9I GGNCC 3 cut(s) 167, 287, 1142
AsuC2I CCSGG 4 cut(s) 165, 290, 321, 798
AsuHPI GGTGA 3 cut(s) 229, 309, 1360
AsuNHI GCTAGC 1 cut(s) 404
AvaI CYCGRG 1 cut(s) 66
AvaII GGWCC 2 cut(s) 167, 1142
BaeGI GKGCMC 1 cut(s) 1676
BaeI ACNNNNGTAYC 2 cut(s) 408, 441
BalI TGGCCA 1 cut(s) 594
BanI GGYRCC 2 cut(s) 416, 541
BanII GRGCYC 2 cut(s) 184, 468
BbrPI CACGTG 1 cut(s) 1435
BbsI GAAGAC 1 cut(s) 1731
Bbv12I GWGCWC 2 cut(s) 184, 726
BbvI GCAGC 1 cut(s) 1156
BccI CCATC 7 cut(s) 278, 428, 776, 1210, 1375, 1554, 1754
BcgI CGANNNNNNTGC 2 cut(s) 557, 591
BciT130I CCWGG 2 cut(s) 600, 1593
BciVI GTATCC 2 cut(s) 187, 669
BcnI CCSGG 4 cut(s) 165, 290, 321, 798
BcoDI GTCTC 2 cut(s) 1618, 1676
BfaI CTAG 4 cut(s) 405, 897, 1751, 1763
BfmI CTRYAG 2 cut(s) 44, 879
BfrI CTTAAG 1 cut(s) 641
BfuI GTATCC 2 cut(s) 187, 669
BisI GCNGC 2 cut(s) 1170, 1277
BlpI GCTNAGC 2 cut(s) 720, 1036
BlsI GCNGC 2 cut(s) 1171, 1278
Bme1390I CCNGG 6 cut(s) 165, 290, 321, 600, 798, 1593
Bme18I GGWCC 2 cut(s) 167, 1142
BmeT110I CYCGRG 1 cut(s) 66
BmgT120I GGNCC 3 cut(s) 167, 287, 1142
BmiI GGNNCC 6 cut(s) 168, 418, 543, 754, 1144, 1411
BmrFI CCNGG 6 cut(s) 165, 290, 321, 600, 798, 1593
BmsI GCATC 4 cut(s) 565, 685, 901, 1344
BmtI GCTAGC 1 cut(s) 408
BpiI GAAGAC 1 cut(s) 1731
BplI GAGNNNNNCTC 2 cut(s) 1481, 1513
BpmI CTGGAG 1 cut(s) 1116
Bpu10I CCTNAGC 1 cut(s) 462
Bpu1102I GCTNAGC 2 cut(s) 720, 1036
BpuEI CTTGAG 1 cut(s) 981
BpuMI CCSGG 4 cut(s) 165, 290, 321, 798
BsaAI YACGTR 3 cut(s) 871, 1435, 1531
BsaJI CCNNGG 5 cut(s) 220, 275, 599, 796, 1336
BsaWI WCCGGW 1 cut(s) 16
BsaXI ACNNNNNCTCC 2 cut(s) 1401, 1431
Bsc4I CCNNNNNNNGG 6 cut(s) 246, 440, 601, 1139, 1556, 1702
Bse118I RCCGGY 1 cut(s) 1412
Bse1I ACTGG 2 cut(s) 251, 398
BseBI CCWGG 2 cut(s) 600, 1593
BseDI CCNNGG 5 cut(s) 220, 275, 599, 796, 1336
BseGI GGATG 6 cut(s) 39, 817, 913, 1423, 1565, 1746
BseLI CCNNNNNNNGG 6 cut(s) 246, 440, 601, 1139, 1556, 1702
BseMII CTCAG 4 cut(s) 453, 711, 903, 1671
BseNI ACTGG 2 cut(s) 251, 398
BseRI GAGGAG 1 cut(s) 491
BseSI GKGCMC 1 cut(s) 1676
BseXI GCAGC 1 cut(s) 1156
BsgI GTGCAG 1 cut(s) 1111
BshFI GGCC 2 cut(s) 288, 594
BshNI GGYRCC 2 cut(s) 416, 541
BsiHKAI GWGCWC 2 cut(s) 184, 726
BsiHKCI CYCGRG 1 cut(s) 66
BsiSI CCGG 7 cut(s) 17, 164, 290, 320, 798, 1413, 1703
BslFI GGGAC 4 cut(s) 1049, 1128, 1354, 1508
BslI CCNNNNNNNGG 6 cut(s) 246, 440, 601, 1139, 1556, 1702
BsmAI GTCTC 2 cut(s) 1618, 1676
BsmFI GGGAC 4 cut(s) 1049, 1128, 1354, 1508
BsnI GGCC 2 cut(s) 288, 594
BsoBI CYCGRG 1 cut(s) 66
Bsp1286I GDGCHC 4 cut(s) 184, 468, 726, 1676
Bsp143I GATC 2 cut(s) 494, 1519
Bsp1720I GCTNAGC 2 cut(s) 720, 1036
Bsp19I CCATGG 1 cut(s) 275
BspACI CCGC 1 cut(s) 1277
BspANI GGCC 2 cut(s) 288, 594
BspCNI CTCAG 4 cut(s) 454, 712, 902, 1672
BspLI GGNNCC 6 cut(s) 168, 418, 543, 754, 1144, 1411
BspOI GCTAGC 1 cut(s) 408
BspPI GGATC 2 cut(s) 502, 1514
BspQI GCTCTTC 1 cut(s) 56
BspT107I GGYRCC 2 cut(s) 416, 541
BspTI CTTAAG 1 cut(s) 641
BsrFI RCCGGY 1 cut(s) 1412
BsrI ACTGG 2 cut(s) 251, 398
BssAI RCCGGY 1 cut(s) 1412
BssECI CCNNGG 5 cut(s) 220, 275, 599, 796, 1336
BssMI GATC 2 cut(s) 494, 1519
BssT1I CCWWGG 3 cut(s) 220, 275, 1336
Bst2UI CCWGG 2 cut(s) 600, 1593
Bst4CI ACNGT 9 cut(s) 12, 370, 389, 416, 736, 752, 883, 1333, 1375
Bst6I CTCTTC 2 cut(s) 56, 468
BstAFI CTTAAG 1 cut(s) 641
BstBAI YACGTR 3 cut(s) 871, 1435, 1531
BstC8I GCNNGC 7 cut(s) 184, 381, 406, 435, 1174, 1221, 1414
BstDEI CTNAG 7 cut(s) 462, 720, 889, 1036, 1086, 1445, 1680
BstDSI CCRYGG 1 cut(s) 275
BstF5I GGATG 6 cut(s) 39, 817, 913, 1423, 1565, 1746
BstKTI GATC 2 cut(s) 497, 1522
BstMAI GTCTC 2 cut(s) 1618, 1676
BstMBI GATC 2 cut(s) 494, 1519
BstNI CCWGG 2 cut(s) 600, 1593
BstNSI RCATGY 2 cut(s) 383, 1152
BstSCI CCNGG 6 cut(s) 163, 288, 319, 598, 796, 1591
BstSFI CTRYAG 2 cut(s) 44, 879
BstSLI GKGCMC 1 cut(s) 1676
BstSNI TACGTA 1 cut(s) 1531
BstV1I GCAGC 1 cut(s) 1156
BstV2I GAAGAC 1 cut(s) 1731
BstX2I RGATCY 1 cut(s) 494
BstXI CCANNNNNNTGG 1 cut(s) 1599
BstYI RGATCY 1 cut(s) 494
BsuI GTATCC 2 cut(s) 187, 669
BsuRI GGCC 2 cut(s) 288, 594
BtgI CCRYGG 1 cut(s) 275
BtsCI GGATG 6 cut(s) 39, 817, 913, 1423, 1565, 1746
BtsIMutI CAGTG 4 cut(s) 244, 394, 732, 1296
Cac8I GCNNGC 7 cut(s) 184, 381, 406, 435, 1174, 1221, 1414
CaiI CAGNNNCTG 2 cut(s) 393, 1211
Cfr10I RCCGGY 1 cut(s) 1412
Cfr13I GGNCC 3 cut(s) 167, 287, 1142
CseI GACGC 2 cut(s) 1141, 1165
Csp6I GTAC 2 cut(s) 417, 706
CviAII CATG 9 cut(s) 139, 251, 276, 380, 745, 985, 1149, 1439, 1635
CviQI GTAC 2 cut(s) 417, 706
DdeI CTNAG 7 cut(s) 462, 720, 889, 1036, 1086, 1445, 1680
DpnI GATC 2 cut(s) 496, 1521
DpnII GATC 2 cut(s) 494, 1519
EaeI YGGCCR 1 cut(s) 592
Eam1104I CTCTTC 2 cut(s) 56, 468
EarI CTCTTC 2 cut(s) 56, 468
Ecl136II GAGCTC 1 cut(s) 182
Eco105I TACGTA 1 cut(s) 1531
Eco130I CCWWGG 3 cut(s) 220, 275, 1336
Eco24I GRGCYC 2 cut(s) 184, 468
Eco47I GGWCC 2 cut(s) 167, 1142
Eco53kI GAGCTC 1 cut(s) 182
Eco57I CTGAAG 1 cut(s) 1725
Eco72I CACGTG 1 cut(s) 1435
Eco88I CYCGRG 1 cut(s) 66
EcoICRI GAGCTC 1 cut(s) 182
EcoO109I RGGNCCY 1 cut(s) 1142
EcoRI GAATTC 1 cut(s) 625
EcoRII CCWGG 2 cut(s) 598, 1591
EcoT14I CCWWGG 3 cut(s) 220, 275, 1336
EcoT38I GRGCYC 2 cut(s) 184, 468
ErhI CCWWGG 3 cut(s) 220, 275, 1336
FaeI CATG 9 cut(s) 142, 254, 279, 383, 748, 988, 1152, 1442, 1638
FaqI GGGAC 4 cut(s) 1049, 1128, 1354, 1508
FatI CATG 9 cut(s) 138, 250, 275, 379, 744, 984, 1148, 1438, 1634
FauNDI CATATG 1 cut(s) 1617
FblI GTMKAC 1 cut(s) 5
Fnu4HI GCNGC 2 cut(s) 1170, 1277
FokI GGATG 6 cut(s) 26, 824, 920, 1410, 1572, 1733
FriOI GRGCYC 2 cut(s) 184, 468
Fsp4HI GCNGC 2 cut(s) 1170, 1277
FspBI CTAG 4 cut(s) 405, 897, 1751, 1763
GluI GCNGC 2 cut(s) 1170, 1277
GsuI CTGGAG 1 cut(s) 1116
HaeIII GGCC 2 cut(s) 288, 594
HapII CCGG 7 cut(s) 17, 164, 290, 320, 798, 1413, 1703
HgaI GACGC 2 cut(s) 1141, 1165
Hin1II CATG 9 cut(s) 142, 254, 279, 383, 748, 988, 1152, 1442, 1638
HincII GTYRAC 3 cut(s) 6, 886, 1156
HindII GTYRAC 3 cut(s) 6, 886, 1156
HindIII AAGCTT 1 cut(s) 1033
HinfI GANTC 8 cut(s) 120, 128, 835, 1304, 1420, 1496, 1575, 1646
HpaI GTTAAC 1 cut(s) 886
HpaII CCGG 7 cut(s) 17, 164, 290, 320, 798, 1413, 1703
HphI GGTGA 3 cut(s) 229, 309, 1360
Hpy166II GTNNAC 8 cut(s) 6, 109, 151, 214, 412, 649, 886, 1156
Hpy188I TCNGA 4 cut(s) 675, 955, 1207, 1574
Hpy188III TCNNGA 7 cut(s) 170, 461, 905, 971, 998, 1021, 1133
Hpy8I GTNNAC 8 cut(s) 6, 109, 151, 214, 412, 649, 886, 1156
Hpy99I CGWCG 1 cut(s) 428
HpyAV CCTTC 5 cut(s) 356, 989, 1339, 1516, 1725
HpyCH4III ACNGT 9 cut(s) 12, 370, 389, 416, 736, 752, 883, 1333, 1375
HpyCH4IV ACGT 6 cut(s) 870, 1080, 1434, 1530, 1538, 1725
HpyCH4V TGCA 8 cut(s) 79, 698, 713, 920, 1128, 1219, 1432, 1438
HpyF3I CTNAG 7 cut(s) 462, 720, 889, 1036, 1086, 1445, 1680
HpySE526I ACGT 6 cut(s) 870, 1080, 1434, 1530, 1538, 1725
Hsp92II CATG 9 cut(s) 142, 254, 279, 383, 748, 988, 1152, 1442, 1638
KpnI GGTACC 1 cut(s) 420
KroI GCCGGC 1 cut(s) 1412
KroNI GCCGGC 1 cut(s) 1414
KspAI GTTAAC 1 cut(s) 886
Kzo9I GATC 2 cut(s) 494, 1519
LguI GCTCTTC 1 cut(s) 56
LmnI GCTCC 5 cut(s) 623, 843, 964, 1409, 1698
Lsp1109I GCAGC 1 cut(s) 1156
LweI GCATC 4 cut(s) 565, 685, 901, 1344
MaeI CTAG 4 cut(s) 405, 897, 1751, 1763
MaeII ACGT 6 cut(s) 870, 1080, 1434, 1530, 1538, 1725
MaeIII GTNAC 7 cut(s) 298, 389, 1076, 1081, 1099, 1348, 1721
MalI GATC 2 cut(s) 496, 1521
MboI GATC 2 cut(s) 494, 1519
MboII GAAGA 7 cut(s) 43, 371, 485, 659, 662, 1675, 1731
MfeI CAATTG 2 cut(s) 347, 714
MflI RGATCY 1 cut(s) 494
MhlI GDGCHC 4 cut(s) 184, 468, 726, 1676
MlsI TGGCCA 1 cut(s) 594
MluNI TGGCCA 1 cut(s) 594
MlyI GAGTC 5 cut(s) 114, 137, 829, 1490, 1655
MmeI TCCRAC 1 cut(s) 1137
MnlI CCTC 7 cut(s) 97, 469, 1120, 1399, 1485, 1533, 1710
Mox20I TGGCCA 1 cut(s) 594
MroNI GCCGGC 1 cut(s) 1412
MroXI GAANNNNTTC 2 cut(s) 978, 1481
MscI TGGCCA 1 cut(s) 594
MseI TTAA 9 cut(s) 99, 207, 642, 681, 885, 1197, 1290, 1328, 1777
MslI CAYNNNNRTG 2 cut(s) 249, 1437
Msp20I TGGCCA 1 cut(s) 594
MspCI CTTAAG 1 cut(s) 641
MspI CCGG 7 cut(s) 17, 164, 290, 320, 798, 1413, 1703
MspR9I CCNGG 6 cut(s) 165, 290, 321, 600, 798, 1593
MunI CAATTG 2 cut(s) 347, 714
MvaI CCWGG 2 cut(s) 600, 1593
NaeI GCCGGC 1 cut(s) 1414
NciI CCSGG 4 cut(s) 165, 290, 321, 798
NcoI CCATGG 1 cut(s) 275
NdeI CATATG 1 cut(s) 1617
NdeII GATC 2 cut(s) 494, 1519
NgoMIV GCCGGC 1 cut(s) 1412
NheI GCTAGC 1 cut(s) 404
NlaIII CATG 9 cut(s) 142, 254, 279, 383, 748, 988, 1152, 1442, 1638
NlaIV GGNNCC 6 cut(s) 168, 418, 543, 754, 1144, 1411
NmeAIII GCCGAG 1 cut(s) 1513
NmuCI GTSAC 5 cut(s) 298, 389, 1076, 1348, 1721
NspI RCATGY 2 cut(s) 383, 1152
PaeI GCATGC 1 cut(s) 383
PaeR7I CTCGAG 1 cut(s) 66
PciSI GCTCTTC 1 cut(s) 56
PcsI WCGNNNNNNNCGW 2 cut(s) 420, 1349
PdiI GCCGGC 1 cut(s) 1414
PdmI GAANNNNTTC 2 cut(s) 978, 1481
PfeI GAWTC 3 cut(s) 1304, 1420, 1575
PflMI CCANNNNNTGG 2 cut(s) 246, 1556
PkrI GCNGC 2 cut(s) 1171, 1278
PleI GAGTC 5 cut(s) 114, 136, 829, 1490, 1654
PmaCI CACGTG 1 cut(s) 1435
PmlI CACGTG 1 cut(s) 1435
PpsI GAGTC 5 cut(s) 114, 136, 829, 1490, 1654
Ppu21I YACGTR 3 cut(s) 871, 1435, 1531
PpuMI RGGWCCY 1 cut(s) 1142
Psp124BI GAGCTC 1 cut(s) 184
Psp1406I AACGTT 1 cut(s) 1538
Psp5II RGGWCCY 1 cut(s) 1142
Psp6I CCWGG 2 cut(s) 598, 1591
PspCI CACGTG 1 cut(s) 1435
PspGI CCWGG 2 cut(s) 598, 1591
PspN4I GGNNCC 6 cut(s) 168, 418, 543, 754, 1144, 1411
PspPI GGNCC 3 cut(s) 167, 287, 1142
PspPPI RGGWCCY 1 cut(s) 1142
PspXI VCTCGAGB 1 cut(s) 66
PsrI GAACNNNNNNTAC 2 cut(s) 316, 348
PstNI CAGNNNCTG 2 cut(s) 393, 1211
PsuI RGATCY 1 cut(s) 494
RsaI GTAC 2 cut(s) 418, 707
RsaNI GTAC 2 cut(s) 417, 706
RseI CAYNNNNRTG 2 cut(s) 249, 1437
SacI GAGCTC 1 cut(s) 184
SalI GTCGAC 1 cut(s) 4
SapI GCTCTTC 1 cut(s) 56
SaqAI TTAA 9 cut(s) 99, 207, 642, 681, 885, 1197, 1290, 1328, 1777
SatI GCNGC 2 cut(s) 1170, 1277
Sau3AI GATC 2 cut(s) 494, 1519
Sau96I GGNCC 3 cut(s) 167, 287, 1142
SchI GAGTC 5 cut(s) 114, 137, 829, 1490, 1655
ScrFI CCNGG 6 cut(s) 165, 290, 321, 600, 798, 1593
SduI GDGCHC 4 cut(s) 184, 468, 726, 1676
SfaNI GCATC 4 cut(s) 565, 685, 901, 1344
SfcI CTRYAG 2 cut(s) 44, 879
Sfr274I CTCGAG 1 cut(s) 66
SinI GGWCC 2 cut(s) 167, 1142
SlaI CTCGAG 1 cut(s) 66
SmiMI CAYNNNNRTG 2 cut(s) 249, 1437
SmlI CTYRAG 3 cut(s) 66, 641, 996
SmoI CTYRAG 3 cut(s) 66, 641, 996
SnaBI TACGTA 1 cut(s) 1531
SphI GCATGC 1 cut(s) 383
SsiI CCGC 1 cut(s) 1277
SspI AATATT 1 cut(s) 1186
SspMI CTAG 4 cut(s) 405, 897, 1751, 1763
SstI GAGCTC 1 cut(s) 184
StyD4I CCNGG 6 cut(s) 163, 288, 319, 598, 796, 1591
StyI CCWWGG 3 cut(s) 220, 275, 1336
TaaI ACNGT 9 cut(s) 12, 370, 389, 416, 736, 752, 883, 1333, 1375
TaiI ACGT 6 cut(s) 873, 1083, 1437, 1533, 1541, 1728
TaqI TCGA 3 cut(s) 5, 67, 833
TatI WGTACW 1 cut(s) 705
TauI GCSGC 1 cut(s) 1279
TfiI GAWTC 3 cut(s) 1304, 1420, 1575
Tru1I TTAA 9 cut(s) 99, 207, 642, 681, 885, 1197, 1290, 1328, 1777
Tru9I TTAA 9 cut(s) 99, 207, 642, 681, 885, 1197, 1290, 1328, 1777
TscAI CASTG 4 cut(s) 251, 394, 739, 1303
TseFI GTSAC 5 cut(s) 298, 389, 1076, 1348, 1721
TseI GCWGC 1 cut(s) 1169
Tsp45I GTSAC 5 cut(s) 298, 389, 1076, 1348, 1721
TspDTI ATGAA 7 cut(s) 43, 486, 1006, 1269, 1296, 1383, 1531
TspRI CASTG 4 cut(s) 251, 394, 739, 1303
Van91I CCANNNNNTGG 2 cut(s) 246, 1556
Vha464I CTTAAG 1 cut(s) 641
VpaK11BI GGWCC 2 cut(s) 167, 1142
XapI RAATTY 5 cut(s) 293, 328, 511, 625, 829
XceI RCATGY 2 cut(s) 383, 1152
XcmI CCANNNNNNNNNTGG 3 cut(s) 227, 273, 1557
XhoI CTCGAG 1 cut(s) 66
XmiI GTMKAC 1 cut(s) 5
XmnI GAANNNNTTC 2 cut(s) 978, 1481
XspI CTAG 4 cut(s) 405, 897, 1751, 1763
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.