Rroxscaffold_3G00224250

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0000320
Physical Location & Seq
Forward (+)
7355782 .. 7361792
6011 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00224250.1

Sequence Viewer

Length: 1695 bp
ATGGGTTGTGATGGTGACGAGATCCAGGTTACACAACTCGATCCCAGTAACCAGATAAAACTAAATCTCTGTTTATCTGCCTTAGAGATCCTTCTGATCTGGCCTGCGTTCATCGGTGAAGACCCTTCTAGCTTCCCAATTCAGATTCCCATGCGCAATGTCCCCGCCCCACTTAAATCTTGGTTCCGCCTCTGGGTGTATAGAATAATTTCCGTGGAGAAGGTAAGGGAGAAAAACTTCACCAAACTGTGTTCAACAAAGAGCATCTTGGTAGTAAACGATAGTTTTCCAGGTCCAGAGATACGAGTTCACAAAGGAGATACAGTTTACGTTAATGTTCATAACCAAGGATATTATGGTTTCACTATTCACTGGCATGGGATAAAACAACCAAGAAATCCGTGGTCAGATGGTCCAGAGTACATCACGCAGTGTCCAATTCTTCCCGGAACAAATTTCACTTATGAAATCTCTTTATCCACTGAAGAAGGAACTTTATGGTGGCATGCTCATAGTGATTGGACAAGAGCCACTGTTCATGGTGCGATTGTCATCTTACCAAGTGTAGGAACCACATTTCCATTTCCTCAACCGGATGAAGATGAGACCATTATAATTGCATCCTGGTACGAGGGAGATATCAAAGAACTAGTTGATGAGGCTATGGATGATGGTAGCGACTTGCCCCATTCAGATGCTTATACAATAAATGGCGAACCAGGAGACTTTTGTCCATGCTCCAGTGAAACAACATATCATCGAATGGTTGACTACGGCAAGACCTATCTTCTTCGTATAGTCAACGCAAACATCAACGCAGAACACTTCTTTGCAGTTGCTGAACACAATCTCACCGTTGTTGGCCTAGACGGAGCATATTTAAAACCGATAGACACAACATACATAGTCATCAGTGCTGGACAAGCGATGGATGTCCTCCTCAAAGCAAACCAATCTCCTGGCCAATACTATATAGCTGCTAGACAATACTCGAGTGAGGATGCAGAAGTAACTGGCTTTGACCACGCGAATGTTACTGCAATCCTTGAATACAGAGGCAACTACACATATCAGACACCTCCTTCCTTTCCAACTACTCTTCCCATGTACTTGGACTATCCAGCAGCAATAAAATTCACCTCCCGGATTAAAAGCTTGGCGACCCCCAAATACCCTACAGATGTCCCATTAAATGTTACTACTAAAATGTATATAACCGTTTCTATGAATTATCTTCCTTGTTCACATCCAGGCTGTGAAACAACTCAACAAATCTCTTCAAGCATGAATAATGTTAGCTGGATTAACCCAACAACAGATGTCCTTAGAGCCTACTACAGGAACATTAGTGGGGTTTACACATCAGATTTTCCAGACCAACCGCCTGCTTATTATAACTTCACAGAGGAAACTTTCGTGGATAATATTGTGTACACTGTCCAAGGGACAAAGGTGAAGATGTTGAATTATAACGAATCAGTTCAAATTGTGTTCCAAGGGACTAATGTGATGAAGGGCTCTGTTAATCATCCAATGCATATGCATGGATATAGCTTCTACGTGGTTGGATCTGGTTATGGAAATTATGACGATGAGAATGATCCAAAGGGATTTAATTTTGTTGATCCTCCTAAAGTAACTACCTTTGGAGTTCCCAAAAATGGCTGGGGTGTGGTTTTGGCACTGTCATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00053 ko01100 map00053 map01100
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

564

Amino Acids

63.36

Weight (kDa)

4.99

Isoelectric Point (pI)

30.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_3 PF07732 75 - 187 5.1e-40 Multicopper oxidase
Cu-oxidase PF00394 201 - 354 5.1e-37 Multicopper oxidase
Cu-oxidase_2 PF07731 457 - 559 2.8e-20 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000320)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g12640 FvH4_2g18980 FvH4_2g18990 FvH4_2g40270 FvH4_2g40270 FvH4_4g22740 FvH4_4g22740 FvH4_5g35760 FvH4_5g35760 FvH4_5g35760 FvH4_5g35760 FvH4_5g35760 FvH4_5g35760 FvH4_5g35760 FvH4_5g35760 FvH4_5g36460 FvH4_5g36460 FvH4_6g39950 FvH4_6g39970 FvH4_6g39983 FvH4_6g39990
rosa_chinensis RchiOBHm_Chr2g0154481 RchiOBHm_Chr2g0154511 RchiOBHm_Chr4g0428961 RchiOBHm_Chr6g0252431 RchiOBHm_Chr6g0273301 RchiOBHm_Chr6g0273631 RchiOBHm_Chr6g0273651 RchiOBHm_Chr6g0284751 RchiOBHm_Chr6g0284761 RchiOBHm_Chr6g0300491 RchiOBHm_Chr7g0237421 RchiOBHm_Chr7g0238581
rosa_laevigata RLG00000000906 RLG00000001006 RLG00000007110 RLG00000011333 RLG00000012721 RLG00000013605 RLG00000013626 RLG00000020780 RLG00000020782 RLG00000020783 RLG00000020786
rosa_multiflora Rmu_co8321753.1_g000001 Rmu_sc0000239.1_g000020 Rmu_sc0000686.1_g000001 Rmu_sc0000686.1_g000003 Rmu_sc0000686.1_g000005 Rmu_sc0001476.1_g000011 Rmu_sc0002231.1_g000002 Rmu_sc0002231.1_g000016 Rmu_sc0002690.1_g000003 Rmu_sc0002717.1_g000015 Rmu_sc0002923.1_g000026 Rmu_sc0003808.1_g000016 Rmu_sc0006475.1_g000008 Rmu_sc0006475.1_g000012 Rmu_sc0006475.1_g000018 Rmu_sc0014815.1_g000004 Rmu_sc0015313.1_g000012
rosa_roxburghii Rroxscaffold_2G00094270 Rroxscaffold_2G00094290 Rroxscaffold_3G00222950 Rroxscaffold_3G00224250 Rroxscaffold_5G00370630 Rroxscaffold_7G00167740 Rroxscaffold_7G00184410 Rroxscaffold_7G00195190 Rroxscaffold_7G00195460
rosa_rugosa Rorug02G0444600 Rorug04G0231100 Rorug04G0231200 Rorug06G0074000 Rorug06G0076800 Rorug06G0166400 Rorug06G0166500 Rorug06G0166600 Rorug06G0296400 Rorug07G0303000
rosa_samantha Rh2CG494600 Rh2CG494700 Rh2DG530800 Rh2DG530900 Rh2DG531000 Rh4AG287500 Rh4AG403800 Rh4BG293400 Rh4BG415100 Rh4DG290500 Rh6AG191000 Rh6AG191300 Rh6BG192500 Rh6BG194800 Rh6BG195200 Rh6BG279500 Rh6BG279600 Rh6DG183900 Rh6DG186600 Rh6DG274300 Rh6DG274500 Rh6DG409500 Rh7AG460600 Rh7AG468200 Rh7BG430200
rosa_wichuraiana Rw2G041820 Rw2G041830 Rw2G041840 Rw4G024930 Rw6G016470 Rw6G016720 Rw6G016730 Rw6G024020 Rw6G024030 Rw6G035790 Rw7G038130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 614, 1395, 1470
Acc16I TGCGCA 1 cut(s) 155
AccII CGCG 1 cut(s) 1028
AciI CCGC 3 cut(s) 165, 187, 1382
AclWI GGATC 6 cut(s) 16, 35, 82, 1576, 1595, 1619
AcoI YGGCCR 1 cut(s) 961
AcsI RAATTY 2 cut(s) 454, 1133
AcuI CTGAAG 1 cut(s) 504
AdeI CACNNNGTG 1 cut(s) 432
AfaI GTAC 4 cut(s) 422, 629, 1109, 1433
AfiI CCNNNNNNNGG 4 cut(s) 193, 566, 1338, 1661
AgsI TTSAA 5 cut(s) 255, 1049, 1281, 1465, 1484
AhlI ACTAGT 1 cut(s) 649
AjnI CCWGG 6 cut(s) 24, 289, 623, 718, 958, 1249
AloI GAACNNNNNNTCC 2 cut(s) 562, 594
AluBI AGCT 5 cut(s) 132, 977, 1155, 1299, 1554
AluI AGCT 5 cut(s) 132, 977, 1155, 1299, 1554
Alw26I GTCTC 2 cut(s) 599, 717
AlwI GGATC 6 cut(s) 16, 35, 82, 1576, 1595, 1619
AlwNI CAGNNNCTG 1 cut(s) 839
Ama87I CYCGRG 1 cut(s) 991
AoxI GGCC 3 cut(s) 101, 862, 961
ApeKI GCWGC 2 cut(s) 977, 1124
ApoI RAATTY 2 cut(s) 454, 1133
Asp700I GAANNNNTTC 3 cut(s) 208, 236, 1479
AspLEI GCGC 1 cut(s) 156
AspS9I GGNCC 2 cut(s) 293, 413
AsuC2I CCSGG 2 cut(s) 447, 1144
AsuHPI GGTGA 6 cut(s) 26, 128, 232, 844, 1129, 1465
AvaI CYCGRG 1 cut(s) 991
AvaII GGWCC 2 cut(s) 293, 413
BalI TGGCCA 1 cut(s) 963
BanII GRGCYC 1 cut(s) 1520
BbsI GAAGAC 1 cut(s) 126
BbvI GCAGC 2 cut(s) 964, 1136
BccI CCATC 4 cut(s) 5, 404, 665, 922
BceAI ACGGC 1 cut(s) 790
BciT130I CCWGG 6 cut(s) 26, 291, 625, 720, 960, 1251
BcnI CCSGG 2 cut(s) 447, 1144
BcoDI GTCTC 2 cut(s) 599, 717
BcuI ACTAGT 1 cut(s) 649
BfaI CTAG 4 cut(s) 129, 650, 866, 981
BfmI CTRYAG 2 cut(s) 1176, 1336
BisI GCNGC 2 cut(s) 978, 1125
BlsI GCNGC 2 cut(s) 979, 1126
Bme1390I CCNGG 8 cut(s) 26, 291, 447, 625, 720, 960, 1144, 1251
Bme18I GGWCC 2 cut(s) 293, 413
BmeT110I CYCGRG 1 cut(s) 991
BmgT120I GGNCC 2 cut(s) 293, 413
BmiI GGNNCC 2 cut(s) 185, 571
BmrFI CCNGG 8 cut(s) 26, 291, 447, 625, 720, 960, 1144, 1251
BmrI ACTGGG 1 cut(s) 39
BmsI GCATC 4 cut(s) 273, 629, 685, 991
BmuI ACTGGG 1 cut(s) 39
BoxI GACNNNNGTC 1 cut(s) 729
BpiI GAAGAC 1 cut(s) 126
BpmI CTGGAG 1 cut(s) 724
BpuMI CCSGG 2 cut(s) 447, 1144
BsaAI YACGTR 1 cut(s) 1561
BsaBI GATNNNNATC 1 cut(s) 551
BsaI GGTCTC 1 cut(s) 599
BsaJI CCNNGG 5 cut(s) 213, 346, 401, 1441, 1495
BsaWI WCCGGW 1 cut(s) 592
BsaXI ACNNNNNCTCC 2 cut(s) 309, 339
Bsc4I CCNNNNNNNGG 4 cut(s) 193, 566, 1338, 1661
Bse1I ACTGG 4 cut(s) 45, 377, 741, 1018
Bse3DI GCAATG 1 cut(s) 163
Bse8I GATNNNNATC 1 cut(s) 551
BseBI CCWGG 6 cut(s) 26, 291, 625, 720, 960, 1251
BseDI CCNNGG 5 cut(s) 213, 346, 401, 1441, 1495
BseGI GGATG 7 cut(s) 601, 620, 673, 937, 1006, 1246, 1528
BseJI GATNNNNATC 1 cut(s) 551
BseLI CCNNNNNNNGG 4 cut(s) 193, 566, 1338, 1661
BseMI GCAATG 1 cut(s) 163
BseNI ACTGG 4 cut(s) 45, 377, 741, 1018
BseRI GAGGAG 1 cut(s) 929
BseXI GCAGC 2 cut(s) 964, 1136
BseYI CCCAGC 1 cut(s) 1665
Bsh1236I CGCG 1 cut(s) 1028
BshFI GGCC 3 cut(s) 103, 864, 963
BsiHKCI CYCGRG 1 cut(s) 991
BsiSI CCGG 3 cut(s) 447, 593, 1144
BslFI GGGAC 4 cut(s) 146, 1169, 1459, 1513
BslI CCNNNNNNNGG 4 cut(s) 193, 566, 1338, 1661
BsmAI GTCTC 2 cut(s) 599, 717
BsmFI GGGAC 4 cut(s) 146, 1169, 1459, 1513
BsnI GGCC 3 cut(s) 103, 864, 963
Bso31I GGTCTC 1 cut(s) 599
BsoBI CYCGRG 1 cut(s) 991
Bsp1286I GDGCHC 1 cut(s) 1520
Bsp1407I TGTACA 1 cut(s) 1431
Bsp143I GATC 7 cut(s) 21, 40, 87, 96, 1568, 1600, 1624
BspACI CCGC 3 cut(s) 165, 187, 1382
BspANI GGCC 3 cut(s) 103, 864, 963
BspFNI CGCG 1 cut(s) 1028
BspLI GGNNCC 2 cut(s) 185, 571
BspPI GGATC 6 cut(s) 16, 35, 82, 1576, 1595, 1619
BspTNI GGTCTC 1 cut(s) 599
BsrDI GCAATG 1 cut(s) 163
BsrGI TGTACA 1 cut(s) 1431
BsrI ACTGG 4 cut(s) 45, 377, 741, 1018
BssECI CCNNGG 5 cut(s) 213, 346, 401, 1441, 1495
BssMI GATC 7 cut(s) 21, 40, 87, 96, 1568, 1600, 1624
BssT1I CCWWGG 3 cut(s) 346, 1441, 1495
Bst2UI CCWGG 6 cut(s) 26, 291, 625, 720, 960, 1251
Bst4CI ACNGT 7 cut(s) 249, 325, 535, 856, 1219, 1438, 1686
Bst6I CTCTTC 2 cut(s) 1104, 1282
BstAUI TGTACA 1 cut(s) 1431
BstBAI YACGTR 1 cut(s) 1561
BstC8I GCNNGC 3 cut(s) 105, 507, 1386
BstDEI CTNAG 2 cut(s) 82, 1325
BstDSI CCRYGG 2 cut(s) 213, 401
BstENI CCTNNNNNAGG 1 cut(s) 1336
BstF5I GGATG 7 cut(s) 601, 620, 673, 937, 1006, 1246, 1528
BstFNI CGCG 1 cut(s) 1028
BstHHI GCGC 1 cut(s) 156
BstKTI GATC 7 cut(s) 24, 43, 90, 99, 1571, 1603, 1627
BstMAI GTCTC 2 cut(s) 599, 717
BstMBI GATC 7 cut(s) 21, 40, 87, 96, 1568, 1600, 1624
BstMWI GCNNNNNNNGC 1 cut(s) 923
BstNI CCWGG 6 cut(s) 26, 291, 625, 720, 960, 1251
BstNSI RCATGY 1 cut(s) 509
BstPAI GACNNNNGTC 1 cut(s) 729
BstSCI CCNGG 8 cut(s) 24, 289, 445, 623, 718, 958, 1142, 1249
BstSFI CTRYAG 2 cut(s) 1176, 1336
BstUI CGCG 1 cut(s) 1028
BstV1I GCAGC 2 cut(s) 964, 1136
BstV2I GAAGAC 1 cut(s) 126
BstX2I RGATCY 3 cut(s) 21, 87, 1568
BstXI CCANNNNNNTGG 2 cut(s) 959, 1111
BstYI RGATCY 3 cut(s) 21, 87, 1568
BsuRI GGCC 3 cut(s) 103, 864, 963
BtgI CCRYGG 2 cut(s) 213, 401
BtgZI GCGATG 1 cut(s) 941
BtsCI GGATG 7 cut(s) 601, 620, 673, 937, 1006, 1246, 1528
BtsI GCAGTG 1 cut(s) 437
BtsIMutI CAGTG 8 cut(s) 370, 437, 480, 531, 748, 919, 1434, 1682
Cac8I GCNNGC 3 cut(s) 105, 507, 1386
CaiI CAGNNNCTG 1 cut(s) 839
CfoI GCGC 1 cut(s) 156
Cfr13I GGNCC 2 cut(s) 293, 413
Csp6I GTAC 4 cut(s) 421, 628, 1108, 1432
CviAII CATG 8 cut(s) 151, 377, 506, 539, 735, 1105, 1285, 1544
CviQI GTAC 4 cut(s) 421, 628, 1108, 1432
DdeI CTNAG 2 cut(s) 82, 1325
DpnI GATC 7 cut(s) 23, 42, 89, 98, 1570, 1602, 1626
DpnII GATC 7 cut(s) 21, 40, 87, 96, 1568, 1600, 1624
DraI TTTAAA 1 cut(s) 882
DraIII CACNNNGTG 1 cut(s) 432
EaeI YGGCCR 1 cut(s) 961
Eam1104I CTCTTC 2 cut(s) 1104, 1282
EarI CTCTTC 2 cut(s) 1104, 1282
EciI GGCGGA 1 cut(s) 176
Eco130I CCWWGG 3 cut(s) 346, 1441, 1495
Eco24I GRGCYC 1 cut(s) 1520
Eco31I GGTCTC 1 cut(s) 599
Eco32I GATATC 1 cut(s) 640
Eco47I GGWCC 2 cut(s) 293, 413
Eco57I CTGAAG 1 cut(s) 504
Eco88I CYCGRG 1 cut(s) 991
EcoNI CCTNNNNNAGG 1 cut(s) 1336
EcoRII CCWGG 6 cut(s) 24, 289, 623, 718, 958, 1249
EcoRV GATATC 1 cut(s) 640
EcoT14I CCWWGG 3 cut(s) 346, 1441, 1495
EcoT22I ATGCAT 2 cut(s) 1539, 1545
EcoT38I GRGCYC 1 cut(s) 1520
ErhI CCWWGG 3 cut(s) 346, 1441, 1495
FaeI CATG 8 cut(s) 154, 380, 509, 542, 738, 1108, 1288, 1547
FalI AAGNNNNNCTT 2 cut(s) 251, 283
FaqI GGGAC 4 cut(s) 146, 1169, 1459, 1513
FatI CATG 8 cut(s) 150, 376, 505, 538, 734, 1104, 1284, 1543
FauI CCCGC 1 cut(s) 172
FauNDI CATATG 1 cut(s) 1539
Fnu4HI GCNGC 2 cut(s) 978, 1125
FokI GGATG 7 cut(s) 607, 608, 680, 944, 1013, 1233, 1515
FriOI GRGCYC 1 cut(s) 1520
Fsp4HI GCNGC 2 cut(s) 978, 1125
FspBI CTAG 4 cut(s) 129, 650, 866, 981
FspI TGCGCA 1 cut(s) 155
GlaI GCGC 1 cut(s) 155
GluI GCNGC 2 cut(s) 978, 1125
GsaI CCCAGC 1 cut(s) 1669
GsuI CTGGAG 1 cut(s) 724
HaeIII GGCC 3 cut(s) 103, 864, 963
HapII CCGG 3 cut(s) 447, 593, 1144
HhaI GCGC 1 cut(s) 156
Hin1II CATG 8 cut(s) 154, 380, 509, 542, 738, 1108, 1288, 1547
Hin6I GCGC 1 cut(s) 154
HinP1I GCGC 1 cut(s) 154
HincII GTYRAC 2 cut(s) 769, 802
HindII GTYRAC 2 cut(s) 769, 802
HindIII AAGCTT 1 cut(s) 1153
HinfI GANTC 2 cut(s) 145, 1475
HpaII CCGG 3 cut(s) 447, 593, 1144
HphI GGTGA 6 cut(s) 26, 128, 232, 844, 1129, 1465
Hpy166II GTNNAC 9 cut(s) 277, 310, 328, 769, 802, 1244, 1357, 1432, 1434
Hpy188I TCNGA 6 cut(s) 96, 144, 409, 694, 1074, 1366
Hpy188III TCNNGA 3 cut(s) 296, 416, 1373
Hpy8I GTNNAC 9 cut(s) 277, 310, 328, 769, 802, 1244, 1357, 1432, 1434
HpyAV CCTTC 6 cut(s) 101, 135, 214, 482, 1092, 1507
HpyCH4III ACNGT 7 cut(s) 249, 325, 535, 856, 1219, 1438, 1686
HpyCH4IV ACGT 2 cut(s) 330, 1560
HpyCH4V TGCA 6 cut(s) 620, 833, 1004, 1040, 1537, 1543
HpyF10VI GCNNNNNNNGC 1 cut(s) 923
HpyF3I CTNAG 2 cut(s) 82, 1325
HpySE526I ACGT 2 cut(s) 330, 1560
Hsp92II CATG 8 cut(s) 154, 380, 509, 542, 738, 1108, 1288, 1547
HspAI GCGC 1 cut(s) 154
Kzo9I GATC 7 cut(s) 21, 40, 87, 96, 1568, 1600, 1624
LmnI GCTCC 2 cut(s) 743, 872
Lsp1109I GCAGC 2 cut(s) 964, 1136
LweI GCATC 4 cut(s) 273, 629, 685, 991
MaeI CTAG 4 cut(s) 129, 650, 866, 981
MaeII ACGT 2 cut(s) 330, 1560
MaeIII GTNAC 7 cut(s) 14, 28, 47, 1009, 1033, 1195, 1636
MalI GATC 7 cut(s) 23, 42, 89, 98, 1570, 1602, 1626
MboI GATC 7 cut(s) 21, 40, 87, 96, 1568, 1600, 1624
MflI RGATCY 3 cut(s) 21, 87, 1568
MhlI GDGCHC 1 cut(s) 1520
MlsI TGGCCA 1 cut(s) 963
MluNI TGGCCA 1 cut(s) 963
MmeI TCCRAC 2 cut(s) 1115, 1546
Mox20I TGGCCA 1 cut(s) 963
Mph1103I ATGCAT 2 cut(s) 1539, 1545
MroXI GAANNNNTTC 3 cut(s) 208, 236, 1479
MscI TGGCCA 1 cut(s) 963
MseI TTAA 8 cut(s) 174, 333, 881, 1149, 1190, 1305, 1524, 1614
MslI CAYNNNNRTG 4 cut(s) 375, 693, 1029, 1542
Msp20I TGGCCA 1 cut(s) 963
MspI CCGG 3 cut(s) 447, 593, 1144
MspR9I CCNGG 8 cut(s) 26, 291, 447, 625, 720, 960, 1144, 1251
MvaI CCWGG 6 cut(s) 26, 291, 625, 720, 960, 1251
MvnI CGCG 1 cut(s) 1028
MwoI GCNNNNNNNGC 1 cut(s) 923
NciI CCSGG 2 cut(s) 447, 1144
NdeI CATATG 1 cut(s) 1539
NdeII GATC 7 cut(s) 21, 40, 87, 96, 1568, 1600, 1624
NlaIII CATG 8 cut(s) 154, 380, 509, 542, 738, 1108, 1288, 1547
NlaIV GGNNCC 2 cut(s) 185, 571
NmuCI GTSAC 1 cut(s) 14
NsbI TGCGCA 1 cut(s) 155
NsiI ATGCAT 2 cut(s) 1539, 1545
NspI RCATGY 1 cut(s) 509
PaeI GCATGC 1 cut(s) 509
PaeR7I CTCGAG 1 cut(s) 991
PdmI GAANNNNTTC 3 cut(s) 208, 236, 1479
PfeI GAWTC 2 cut(s) 145, 1475
PfoI TCCNGGA 2 cut(s) 445, 1142
PkrI GCNGC 2 cut(s) 979, 1126
Ppu21I YACGTR 1 cut(s) 1561
PshAI GACNNNNGTC 1 cut(s) 729
PsiI TTATAA 3 cut(s) 614, 1395, 1470
Psp6I CCWGG 6 cut(s) 24, 289, 623, 718, 958, 1249
PspFI CCCAGC 1 cut(s) 1665
PspGI CCWGG 6 cut(s) 24, 289, 623, 718, 958, 1249
PspN4I GGNNCC 2 cut(s) 185, 571
PspPI GGNCC 2 cut(s) 293, 413
PspXI VCTCGAGB 1 cut(s) 991
PstNI CAGNNNCTG 1 cut(s) 839
PsuI RGATCY 3 cut(s) 21, 87, 1568
RsaI GTAC 4 cut(s) 422, 629, 1109, 1433
RsaNI GTAC 4 cut(s) 421, 628, 1108, 1432
RseI CAYNNNNRTG 4 cut(s) 375, 693, 1029, 1542
SaqAI TTAA 8 cut(s) 174, 333, 881, 1149, 1190, 1305, 1524, 1614
SatI GCNGC 2 cut(s) 978, 1125
Sau3AI GATC 7 cut(s) 21, 40, 87, 96, 1568, 1600, 1624
Sau96I GGNCC 2 cut(s) 293, 413
ScrFI CCNGG 8 cut(s) 26, 291, 447, 625, 720, 960, 1144, 1251
SduI GDGCHC 1 cut(s) 1520
SfaNI GCATC 4 cut(s) 273, 629, 685, 991
SfcI CTRYAG 2 cut(s) 1176, 1336
Sfr274I CTCGAG 1 cut(s) 991
SinI GGWCC 2 cut(s) 293, 413
SlaI CTCGAG 1 cut(s) 991
SmiMI CAYNNNNRTG 4 cut(s) 375, 693, 1029, 1542
SmlI CTYRAG 1 cut(s) 991
SmoI CTYRAG 1 cut(s) 991
SpeI ACTAGT 1 cut(s) 649
SphI GCATGC 1 cut(s) 509
SsiI CCGC 3 cut(s) 165, 187, 1382
SspI AATATT 1 cut(s) 1426
SspMI CTAG 4 cut(s) 129, 650, 866, 981
StyD4I CCNGG 8 cut(s) 24, 289, 445, 623, 718, 958, 1142, 1249
StyI CCWWGG 3 cut(s) 346, 1441, 1495
TaaI ACNGT 7 cut(s) 249, 325, 535, 856, 1219, 1438, 1686
TaiI ACGT 2 cut(s) 333, 1563
TaqI TCGA 3 cut(s) 39, 760, 992
TatI WGTACW 3 cut(s) 420, 1107, 1431
TfiI GAWTC 2 cut(s) 145, 1475
Tru1I TTAA 8 cut(s) 174, 333, 881, 1149, 1190, 1305, 1524, 1614
Tru9I TTAA 8 cut(s) 174, 333, 881, 1149, 1190, 1305, 1524, 1614
TscAI CASTG 8 cut(s) 377, 437, 487, 538, 748, 919, 1441, 1689
TseFI GTSAC 1 cut(s) 14
TseI GCWGC 2 cut(s) 977, 1124
Tsp45I GTSAC 1 cut(s) 14
TspDTI ATGAA 8 cut(s) 100, 329, 480, 527, 612, 1241, 1301, 1526
TspGWI ACGGA 3 cut(s) 202, 390, 885
TspRI CASTG 8 cut(s) 377, 437, 487, 538, 748, 919, 1441, 1689
VpaK11BI GGWCC 2 cut(s) 293, 413
XagI CCTNNNNNAGG 1 cut(s) 1336
XapI RAATTY 2 cut(s) 454, 1133
XceI RCATGY 1 cut(s) 509
XcmI CCANNNNNNNNNTGG 3 cut(s) 177, 353, 399
XhoI CTCGAG 1 cut(s) 991
XmnI GAANNNNTTC 3 cut(s) 208, 236, 1479
XspI CTAG 4 cut(s) 129, 650, 866, 981
Zsp2I ATGCAT 2 cut(s) 1539, 1545
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.