Lignin degradation and detoxification of lignin-derived products
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 449 |
| AccB1I | GGYRCC | 2 cut(s) | 449, 574 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 279, 1589 |
| AccBSI | CCGCTC | 1 cut(s) | 1445 |
| AccI | GTMKAC | 1 cut(s) | 38 |
| AciI | CCGC | 2 cut(s) | 1310, 1445 |
| AclI | AACGTT | 1 cut(s) | 1571 |
| AclWI | GGATC | 2 cut(s) | 535, 1547 |
| AcoI | YGGCCR | 2 cut(s) | 625, 1446 |
| AcsI | RAATTY | 4 cut(s) | 361, 544, 658, 862 |
| AcuI | CTGAAG | 2 cut(s) | 32, 1758 |
| AcvI | CACGTG | 1 cut(s) | 1468 |
| AfaI | GTAC | 3 cut(s) | 451, 740, 1162 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 279, 634, 1172, 1589, 1735 |
| AflII | CTTAAG | 1 cut(s) | 674 |
| AgsI | TTSAA | 7 cut(s) | 380, 550, 663, 962, 1424, 1543, 1768 |
| AjnI | CCWGG | 3 cut(s) | 631, 829, 1624 |
| AloI | GAACNNNNNNTCC | 4 cut(s) | 10, 42, 469, 501 |
| Alw21I | GWGCWC | 2 cut(s) | 217, 759 |
| Alw26I | GTCTC | 3 cut(s) | 873, 1651, 1709 |
| AlwI | GGATC | 2 cut(s) | 535, 1547 |
| Ama87I | CYCGRG | 1 cut(s) | 99 |
| AoxI | GGCC | 4 cut(s) | 96, 319, 625, 1446 |
| ApeKI | GCWGC | 2 cut(s) | 876, 1202 |
| ApoI | RAATTY | 4 cut(s) | 361, 544, 658, 862 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 1011, 1514 |
| Asp718I | GGTACC | 1 cut(s) | 449 |
| AspS9I | GGNCC | 5 cut(s) | 26, 97, 200, 320, 1175 |
| AsuC2I | CCSGG | 3 cut(s) | 198, 323, 354 |
| AsuHPI | GGTGA | 3 cut(s) | 262, 342, 1393 |
| AsuNHI | GCTAGC | 1 cut(s) | 437 |
| AvaI | CYCGRG | 1 cut(s) | 99 |
| AvaII | GGWCC | 3 cut(s) | 26, 200, 1175 |
| AxyI | CCTNAGG | 1 cut(s) | 1052 |
| BaeGI | GKGCMC | 1 cut(s) | 1709 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 441, 474 |
| BalI | TGGCCA | 1 cut(s) | 627 |
| BanI | GGYRCC | 2 cut(s) | 449, 574 |
| BanII | GRGCYC | 2 cut(s) | 217, 501 |
| BbrPI | CACGTG | 1 cut(s) | 1468 |
| BbsI | GAAGAC | 1 cut(s) | 1764 |
| Bbv12I | GWGCWC | 2 cut(s) | 217, 759 |
| BbvI | GCAGC | 2 cut(s) | 888, 1189 |
| BccI | CCATC | 6 cut(s) | 311, 809, 1243, 1408, 1587, 1787 |
| BceAI | ACGGC | 1 cut(s) | 1433 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 590, 624 |
| BciT130I | CCWGG | 3 cut(s) | 633, 831, 1626 |
| BciVI | GTATCC | 2 cut(s) | 220, 702 |
| BcnI | CCSGG | 3 cut(s) | 198, 323, 354 |
| BcoDI | GTCTC | 3 cut(s) | 873, 1651, 1709 |
| BfaI | CTAG | 4 cut(s) | 438, 930, 1784, 1796 |
| BfmI | CTRYAG | 2 cut(s) | 77, 912 |
| BfrI | CTTAAG | 1 cut(s) | 674 |
| BfuI | GTATCC | 2 cut(s) | 220, 702 |
| BglII | AGATCT | 1 cut(s) | 81 |
| BisI | GCNGC | 4 cut(s) | 877, 1203, 1310, 1446 |
| BlpI | GCTNAGC | 2 cut(s) | 753, 1069 |
| BlsI | GCNGC | 4 cut(s) | 878, 1204, 1311, 1447 |
| Bme1390I | CCNGG | 6 cut(s) | 198, 323, 354, 633, 831, 1626 |
| Bme18I | GGWCC | 3 cut(s) | 26, 200, 1175 |
| BmeT110I | CYCGRG | 1 cut(s) | 99 |
| BmgT120I | GGNCC | 5 cut(s) | 26, 97, 200, 320, 1175 |
| BmiI | GGNNCC | 5 cut(s) | 201, 451, 576, 787, 1177 |
| BmrFI | CCNGG | 6 cut(s) | 198, 323, 354, 633, 831, 1626 |
| BmsI | GCATC | 4 cut(s) | 598, 718, 934, 1377 |
| BmtI | GCTAGC | 1 cut(s) | 441 |
| BpiI | GAAGAC | 1 cut(s) | 1764 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1514, 1546 |
| BpmI | CTGGAG | 1 cut(s) | 1149 |
| Bpu10I | CCTNAGC | 1 cut(s) | 495 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 753, 1069 |
| BpuMI | CCSGG | 3 cut(s) | 198, 323, 354 |
| BsaAI | YACGTR | 2 cut(s) | 904, 1468 |
| BsaBI | GATNNNNATC | 1 cut(s) | 86 |
| BsaJI | CCNNGG | 4 cut(s) | 308, 632, 829, 1369 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1434, 1464 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 279, 634, 1172, 1589, 1735 |
| Bse1I | ACTGG | 6 cut(s) | 28, 29, 50, 284, 431, 1280 |
| Bse21I | CCTNAGG | 1 cut(s) | 1052 |
| Bse8I | GATNNNNATC | 1 cut(s) | 86 |
| BseBI | CCWGG | 3 cut(s) | 633, 831, 1626 |
| BseDI | CCNNGG | 4 cut(s) | 308, 632, 829, 1369 |
| BseGI | GGATG | 6 cut(s) | 72, 850, 946, 1456, 1598, 1779 |
| BseJI | GATNNNNATC | 1 cut(s) | 86 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 279, 634, 1172, 1589, 1735 |
| BseMII | CTCAG | 7 cut(s) | 486, 744, 936, 1066, 1492, 1618, 1704 |
| BseNI | ACTGG | 6 cut(s) | 28, 29, 50, 284, 431, 1280 |
| BseRI | GAGGAG | 2 cut(s) | 524, 1722 |
| BseSI | GKGCMC | 1 cut(s) | 1709 |
| BseX3I | CGGCCG | 1 cut(s) | 1446 |
| BseXI | GCAGC | 2 cut(s) | 888, 1189 |
| Bsh1285I | CGRYCG | 1 cut(s) | 1449 |
| BshFI | GGCC | 4 cut(s) | 98, 321, 627, 1448 |
| BshNI | GGYRCC | 2 cut(s) | 449, 574 |
| BsiEI | CGRYCG | 1 cut(s) | 1449 |
| BsiHKAI | GWGCWC | 2 cut(s) | 217, 759 |
| BsiHKCI | CYCGRG | 1 cut(s) | 99 |
| BsiSI | CCGG | 4 cut(s) | 197, 323, 353, 1736 |
| BslFI | GGGAC | 4 cut(s) | 1082, 1161, 1387, 1541 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 279, 634, 1172, 1589, 1735 |
| BsmAI | GTCTC | 3 cut(s) | 873, 1651, 1709 |
| BsmBI | CGTCTC | 1 cut(s) | 873 |
| BsmFI | GGGAC | 4 cut(s) | 1082, 1161, 1387, 1541 |
| BsnI | GGCC | 4 cut(s) | 98, 321, 627, 1448 |
| BsoBI | CYCGRG | 1 cut(s) | 99 |
| Bsp1286I | GDGCHC | 4 cut(s) | 217, 501, 759, 1709 |
| Bsp143I | GATC | 3 cut(s) | 81, 527, 1552 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 753, 1069 |
| Bsp19I | CCATGG | 1 cut(s) | 308 |
| BspACI | CCGC | 2 cut(s) | 1310, 1445 |
| BspANI | GGCC | 4 cut(s) | 98, 321, 627, 1448 |
| BspCNI | CTCAG | 7 cut(s) | 487, 745, 935, 1065, 1491, 1617, 1705 |
| BspLI | GGNNCC | 5 cut(s) | 201, 451, 576, 787, 1177 |
| BspOI | GCTAGC | 1 cut(s) | 441 |
| BspPI | GGATC | 2 cut(s) | 535, 1547 |
| BspT107I | GGYRCC | 2 cut(s) | 449, 574 |
| BspTI | CTTAAG | 1 cut(s) | 674 |
| BsrBI | CCGCTC | 1 cut(s) | 1445 |
| BsrI | ACTGG | 6 cut(s) | 28, 29, 50, 284, 431, 1280 |
| BssECI | CCNNGG | 4 cut(s) | 308, 632, 829, 1369 |
| BssMI | GATC | 3 cut(s) | 81, 527, 1552 |
| BssT1I | CCWWGG | 2 cut(s) | 308, 1369 |
| Bst2UI | CCWGG | 3 cut(s) | 633, 831, 1626 |
| Bst4CI | ACNGT | 9 cut(s) | 45, 156, 403, 449, 785, 916, 1160, 1366, 1408 |
| Bst6I | CTCTTC | 2 cut(s) | 8, 501 |
| BstAFI | CTTAAG | 1 cut(s) | 674 |
| BstBAI | YACGTR | 2 cut(s) | 904, 1468 |
| BstC8I | GCNNGC | 6 cut(s) | 217, 414, 439, 468, 1207, 1254 |
| BstDSI | CCRYGG | 1 cut(s) | 308 |
| BstF5I | GGATG | 6 cut(s) | 72, 850, 946, 1456, 1598, 1779 |
| BstKTI | GATC | 3 cut(s) | 84, 530, 1555 |
| BstMAI | GTCTC | 3 cut(s) | 873, 1651, 1709 |
| BstMBI | GATC | 3 cut(s) | 81, 527, 1552 |
| BstMCI | CGRYCG | 1 cut(s) | 1449 |
| BstNI | CCWGG | 3 cut(s) | 633, 831, 1626 |
| BstNSI | RCATGY | 2 cut(s) | 416, 1185 |
| BstSCI | CCNGG | 6 cut(s) | 196, 321, 352, 631, 829, 1624 |
| BstSFI | CTRYAG | 2 cut(s) | 77, 912 |
| BstSLI | GKGCMC | 1 cut(s) | 1709 |
| BstV1I | GCAGC | 2 cut(s) | 888, 1189 |
| BstV2I | GAAGAC | 1 cut(s) | 1764 |
| BstX2I | RGATCY | 2 cut(s) | 81, 527 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1632 |
| BstYI | RGATCY | 2 cut(s) | 81, 527 |
| BstZI | CGGCCG | 1 cut(s) | 1446 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1052 |
| BsuI | GTATCC | 2 cut(s) | 220, 702 |
| BsuRI | GGCC | 4 cut(s) | 98, 321, 627, 1448 |
| BtgI | CCRYGG | 1 cut(s) | 308 |
| BtsCI | GGATG | 6 cut(s) | 72, 850, 946, 1456, 1598, 1779 |
| BtsIMutI | CAGTG | 4 cut(s) | 36, 57, 277, 1329 |
| Cac8I | GCNNGC | 6 cut(s) | 217, 414, 439, 468, 1207, 1254 |
| Cfr13I | GGNCC | 5 cut(s) | 26, 97, 200, 320, 1175 |
| CseI | GACGC | 1 cut(s) | 1198 |
| Csp6I | GTAC | 3 cut(s) | 450, 739, 1161 |
| CviQI | GTAC | 3 cut(s) | 450, 739, 1161 |
| DpnI | GATC | 3 cut(s) | 83, 529, 1554 |
| DpnII | GATC | 3 cut(s) | 81, 527, 1552 |
| EaeI | YGGCCR | 2 cut(s) | 625, 1446 |
| EagI | CGGCCG | 1 cut(s) | 1446 |
| Eam1104I | CTCTTC | 2 cut(s) | 8, 501 |
| EarI | CTCTTC | 2 cut(s) | 8, 501 |
| Ecl136II | GAGCTC | 1 cut(s) | 215 |
| EclXI | CGGCCG | 1 cut(s) | 1446 |
| Eco130I | CCWWGG | 2 cut(s) | 308, 1369 |
| Eco24I | GRGCYC | 2 cut(s) | 217, 501 |
| Eco47I | GGWCC | 3 cut(s) | 26, 200, 1175 |
| Eco52I | CGGCCG | 1 cut(s) | 1446 |
| Eco53kI | GAGCTC | 1 cut(s) | 215 |
| Eco57I | CTGAAG | 2 cut(s) | 32, 1758 |
| Eco72I | CACGTG | 1 cut(s) | 1468 |
| Eco81I | CCTNAGG | 1 cut(s) | 1052 |
| Eco88I | CYCGRG | 1 cut(s) | 99 |
| EcoICRI | GAGCTC | 1 cut(s) | 215 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1175 |
| EcoRI | GAATTC | 1 cut(s) | 658 |
| EcoRII | CCWGG | 3 cut(s) | 631, 829, 1624 |
| EcoT14I | CCWWGG | 2 cut(s) | 308, 1369 |
| EcoT38I | GRGCYC | 2 cut(s) | 217, 501 |
| ErhI | CCWWGG | 2 cut(s) | 308, 1369 |
| Esp3I | CGTCTC | 1 cut(s) | 873 |
| FaqI | GGGAC | 4 cut(s) | 1082, 1161, 1387, 1541 |
| FauNDI | CATATG | 1 cut(s) | 1650 |
| FblI | GTMKAC | 1 cut(s) | 38 |
| Fnu4HI | GCNGC | 4 cut(s) | 877, 1203, 1310, 1446 |
| FokI | GGATG | 6 cut(s) | 59, 857, 953, 1443, 1605, 1766 |
| FriOI | GRGCYC | 2 cut(s) | 217, 501 |
| Fsp4HI | GCNGC | 4 cut(s) | 877, 1203, 1310, 1446 |
| FspBI | CTAG | 4 cut(s) | 438, 930, 1784, 1796 |
| GluI | GCNGC | 4 cut(s) | 877, 1203, 1310, 1446 |
| GsuI | CTGGAG | 1 cut(s) | 1149 |
| HaeIII | GGCC | 4 cut(s) | 98, 321, 627, 1448 |
| HapII | CCGG | 4 cut(s) | 197, 323, 353, 1736 |
| HgaI | GACGC | 1 cut(s) | 1198 |
| HincII | GTYRAC | 3 cut(s) | 39, 919, 1189 |
| HindII | GTYRAC | 3 cut(s) | 39, 919, 1189 |
| HindIII | AAGCTT | 1 cut(s) | 1066 |
| HinfI | GANTC | 8 cut(s) | 161, 1241, 1337, 1453, 1476, 1529, 1608, 1679 |
| HpaI | GTTAAC | 2 cut(s) | 919, 1189 |
| HpaII | CCGG | 4 cut(s) | 197, 323, 353, 1736 |
| HphI | GGTGA | 3 cut(s) | 262, 342, 1393 |
| Hpy166II | GTNNAC | 8 cut(s) | 39, 142, 184, 247, 445, 682, 919, 1189 |
| Hpy188I | TCNGA | 5 cut(s) | 708, 988, 996, 1240, 1607 |
| Hpy188III | TCNNGA | 6 cut(s) | 203, 494, 938, 1004, 1054, 1166 |
| Hpy8I | GTNNAC | 8 cut(s) | 39, 142, 184, 247, 445, 682, 919, 1189 |
| HpyAV | CCTTC | 4 cut(s) | 389, 1022, 1549, 1758 |
| HpyCH4III | ACNGT | 9 cut(s) | 45, 156, 403, 449, 785, 916, 1160, 1366, 1408 |
| HpyCH4IV | ACGT | 6 cut(s) | 695, 903, 1113, 1467, 1571, 1758 |
| HpyCH4V | TGCA | 7 cut(s) | 112, 731, 953, 1205, 1252, 1465, 1471 |
| HpySE526I | ACGT | 6 cut(s) | 695, 903, 1113, 1467, 1571, 1758 |
| KpnI | GGTACC | 1 cut(s) | 453 |
| KspAI | GTTAAC | 2 cut(s) | 919, 1189 |
| Kzo9I | GATC | 3 cut(s) | 81, 527, 1552 |
| LmnI | GCTCC | 4 cut(s) | 656, 997, 1442, 1731 |
| Lsp1109I | GCAGC | 2 cut(s) | 888, 1189 |
| LweI | GCATC | 4 cut(s) | 598, 718, 934, 1377 |
| MaeI | CTAG | 4 cut(s) | 438, 930, 1784, 1796 |
| MaeII | ACGT | 6 cut(s) | 695, 903, 1113, 1467, 1571, 1758 |
| MaeIII | GTNAC | 6 cut(s) | 331, 422, 1109, 1114, 1381, 1754 |
| MalI | GATC | 3 cut(s) | 83, 529, 1554 |
| MbiI | CCGCTC | 1 cut(s) | 1445 |
| MboI | GATC | 3 cut(s) | 81, 527, 1552 |
| MboII | GAAGA | 8 cut(s) | 25, 76, 404, 518, 692, 695, 1708, 1764 |
| MfeI | CAATTG | 1 cut(s) | 380 |
| MflI | RGATCY | 2 cut(s) | 81, 527 |
| MhlI | GDGCHC | 4 cut(s) | 217, 501, 759, 1709 |
| MlsI | TGGCCA | 1 cut(s) | 627 |
| MluNI | TGGCCA | 1 cut(s) | 627 |
| MlyI | GAGTC | 4 cut(s) | 170, 1470, 1523, 1688 |
| MmeI | TCCRAC | 1 cut(s) | 1170 |
| MnlI | CCTC | 9 cut(s) | 130, 502, 1061, 1153, 1432, 1518, 1566, 1740, 1743 |
| Mox20I | TGGCCA | 1 cut(s) | 627 |
| MroXI | GAANNNNTTC | 2 cut(s) | 1011, 1514 |
| MscI | TGGCCA | 1 cut(s) | 627 |
| MseI | TTAA | 9 cut(s) | 132, 240, 675, 918, 1188, 1230, 1323, 1361, 1810 |
| MslI | CAYNNNNRTG | 2 cut(s) | 282, 1470 |
| Msp20I | TGGCCA | 1 cut(s) | 627 |
| MspCI | CTTAAG | 1 cut(s) | 674 |
| MspI | CCGG | 4 cut(s) | 197, 323, 353, 1736 |
| MspR9I | CCNGG | 6 cut(s) | 198, 323, 354, 633, 831, 1626 |
| MunI | CAATTG | 1 cut(s) | 380 |
| MvaI | CCWGG | 3 cut(s) | 633, 831, 1626 |
| NciI | CCSGG | 3 cut(s) | 198, 323, 354 |
| NcoI | CCATGG | 1 cut(s) | 308 |
| NdeI | CATATG | 1 cut(s) | 1650 |
| NdeII | GATC | 3 cut(s) | 81, 527, 1552 |
| NheI | GCTAGC | 1 cut(s) | 437 |
| NlaIV | GGNNCC | 5 cut(s) | 201, 451, 576, 787, 1177 |
| NmeAIII | GCCGAG | 1 cut(s) | 1546 |
| NmuCI | GTSAC | 5 cut(s) | 331, 422, 1109, 1381, 1754 |
| NspI | RCATGY | 2 cut(s) | 416, 1185 |
| PaeI | GCATGC | 1 cut(s) | 416 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 992 |
| PdmI | GAANNNNTTC | 2 cut(s) | 1011, 1514 |
| PfeI | GAWTC | 4 cut(s) | 1241, 1337, 1453, 1608 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 279, 1589 |
| PkrI | GCNGC | 4 cut(s) | 878, 1204, 1311, 1447 |
| PleI | GAGTC | 4 cut(s) | 169, 1470, 1523, 1687 |
| PmaCI | CACGTG | 1 cut(s) | 1468 |
| PmlI | CACGTG | 1 cut(s) | 1468 |
| PpsI | GAGTC | 4 cut(s) | 169, 1470, 1523, 1687 |
| Ppu21I | YACGTR | 2 cut(s) | 904, 1468 |
| PpuMI | RGGWCCY | 1 cut(s) | 1175 |
| Psp124BI | GAGCTC | 1 cut(s) | 217 |
| Psp1406I | AACGTT | 1 cut(s) | 1571 |
| Psp5II | RGGWCCY | 1 cut(s) | 1175 |
| Psp6I | CCWGG | 3 cut(s) | 631, 829, 1624 |
| PspCI | CACGTG | 1 cut(s) | 1468 |
| PspGI | CCWGG | 3 cut(s) | 631, 829, 1624 |
| PspN4I | GGNNCC | 5 cut(s) | 201, 451, 576, 787, 1177 |
| PspPI | GGNCC | 5 cut(s) | 26, 97, 200, 320, 1175 |
| PspPPI | RGGWCCY | 1 cut(s) | 1175 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 349, 381 |
| PsuI | RGATCY | 2 cut(s) | 81, 527 |
| RsaI | GTAC | 3 cut(s) | 451, 740, 1162 |
| RsaNI | GTAC | 3 cut(s) | 450, 739, 1161 |
| RseI | CAYNNNNRTG | 2 cut(s) | 282, 1470 |
| SacI | GAGCTC | 1 cut(s) | 217 |
| SalI | GTCGAC | 1 cut(s) | 37 |
| SaqAI | TTAA | 9 cut(s) | 132, 240, 675, 918, 1188, 1230, 1323, 1361, 1810 |
| SatI | GCNGC | 4 cut(s) | 877, 1203, 1310, 1446 |
| Sau3AI | GATC | 3 cut(s) | 81, 527, 1552 |
| Sau96I | GGNCC | 5 cut(s) | 26, 97, 200, 320, 1175 |
| SchI | GAGTC | 4 cut(s) | 170, 1470, 1523, 1688 |
| ScrFI | CCNGG | 6 cut(s) | 198, 323, 354, 633, 831, 1626 |
| SduI | GDGCHC | 4 cut(s) | 217, 501, 759, 1709 |
| SfaNI | GCATC | 4 cut(s) | 598, 718, 934, 1377 |
| SfcI | CTRYAG | 2 cut(s) | 77, 912 |
| SinI | GGWCC | 3 cut(s) | 26, 200, 1175 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 282, 1470 |
| SmlI | CTYRAG | 1 cut(s) | 674 |
| SmoI | CTYRAG | 1 cut(s) | 674 |
| SphI | GCATGC | 1 cut(s) | 416 |
| SsiI | CCGC | 2 cut(s) | 1310, 1445 |
| SspI | AATATT | 2 cut(s) | 724, 1219 |
| SspMI | CTAG | 4 cut(s) | 438, 930, 1784, 1796 |
| SstI | GAGCTC | 1 cut(s) | 217 |
| StyD4I | CCNGG | 6 cut(s) | 196, 321, 352, 631, 829, 1624 |
| StyI | CCWWGG | 2 cut(s) | 308, 1369 |
| TaaI | ACNGT | 9 cut(s) | 45, 156, 403, 449, 785, 916, 1160, 1366, 1408 |
| TaiI | ACGT | 6 cut(s) | 698, 906, 1116, 1470, 1574, 1761 |
| TaqI | TCGA | 1 cut(s) | 38 |
| TatI | WGTACW | 2 cut(s) | 738, 1160 |
| TauI | GCSGC | 2 cut(s) | 1312, 1448 |
| TfiI | GAWTC | 4 cut(s) | 1241, 1337, 1453, 1608 |
| Tru1I | TTAA | 9 cut(s) | 132, 240, 675, 918, 1188, 1230, 1323, 1361, 1810 |
| Tru9I | TTAA | 9 cut(s) | 132, 240, 675, 918, 1188, 1230, 1323, 1361, 1810 |
| TscAI | CASTG | 4 cut(s) | 36, 57, 284, 1336 |
| TseFI | GTSAC | 5 cut(s) | 331, 422, 1109, 1381, 1754 |
| TseI | GCWGC | 2 cut(s) | 876, 1202 |
| Tsp45I | GTSAC | 5 cut(s) | 331, 422, 1109, 1381, 1754 |
| TspDTI | ATGAA | 7 cut(s) | 17, 76, 519, 1302, 1329, 1416, 1564 |
| TspRI | CASTG | 4 cut(s) | 36, 57, 284, 1336 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 279, 1589 |
| Vha464I | CTTAAG | 1 cut(s) | 674 |
| VpaK11BI | GGWCC | 3 cut(s) | 26, 200, 1175 |
| XapI | RAATTY | 4 cut(s) | 361, 544, 658, 862 |
| XceI | RCATGY | 2 cut(s) | 416, 1185 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 306, 1590 |
| XmiI | GTMKAC | 1 cut(s) | 38 |
| XmnI | GAANNNNTTC | 2 cut(s) | 1011, 1514 |
| XspI | CTAG | 4 cut(s) | 438, 930, 1784, 1796 |