RchiOBHm_Chr6g0284751

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000320
Physical Location & Seq
Reverse (-)
48100385 .. 48102883
2499 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ25537

Sequence Viewer

Length: 1791 bp
ATGAAATCCCAAAAGAAAAGGACGGCTATTCTAGTTGATATCTTTCTTGTCCTGCAGTTCTTGATGATCTCTCTGGTTCAAGGCGAAGTACATGTCTACGATTTTGTATTAAGGGAGAAAAACTTCACCAGATTATGCGAAACAAAGAGCATGTTTGTTGTAAACGACATTTTCCCAGGACCGGAGATACGAGTTCATAAAGGGGATATGGTCTATGTAAATGTTTATAATCAAGCATATTATGGTCTCACTATTCACTGGCATGGAATAATGCAACTAAGAAATCCATGGTCAGATGGTCCTGAGTATATCACACAGTGTCCAATCCAACCAGGGACTAATTTCACATACGAGGTCTTGTTATCTACGGAAGAAGGAACCGTATGGTGGCATGCTCATAGTGACTGGACACGAGCTAGCGTTCATGGGGCCATTGTCATCTTGCCTACTGTTGGAAGCACATATCCATTTCCTCAAACCGATGAAGATGAGGTCATTATATTATCATCCTGGTACTTAGGAGATTTGAAAGTACGGGTTGATGAGGCTATGAAAGAAGCCACATCTTTACCCCGTTCTGATGCATACACAATAAATGGGCAGCCGGGGGATCTTTTTCCATGCTCCAAGGGGACAGCATATCGTCTAAAAGTGGATTATGCCAAGACCTATCTTCTTCGTATAGTCAATGCAAACATAGATTCATCCCTTTTCTTTGCAGTTGCTCAACACAACCTCACAGTTGTCGGACTAGACGGAGCCTATATCAAACCCATATACACAACCTACATAGTCATAAGCCCTGGACAAACTATGGATGTTTTGCTCAAAACTGATCAGGTTCTCGGGCGCTATTACATGGCTGGTAGACATTTCTCTAGTGATAATGCCAAAGTTTCTCAATTTGGCCATGATGTTACTGCAATCTTGGAATACAATGGCGACTACACTTATCCAACATCACCTCTCTTTCCCAAAACACTTCCTATGTACTCAGACTTTTCAGCAGCATTAAACTTCACTTACCAAATTAAAAGCTTAGCTTCCCCAGAATATCCAATAAATGTCCCACCAGACAATGACATTACTACCAGAATGTTTATAACAGCTTCCATGAATTCACTTTACTGTGAAGATTCAGGCTTCTTGAGCTGCACAGAGATACCAATCGCTACAAGCGTAAATAACATTAGCTGGGTGAACCCGAGGAATACAGATCTCTTGCAAGCTTATTACAGGAACATAAGTGGGGTTTACTCAACTGATTTTCCAGACCAACCGCCTACTTATTATAACTTTACAGACGAAACTTACTCGAATGATACCGTGTTAACAGTACAAGGAACAAAGGTGAAGGTGTTAAGTTATAATGAGTCAGTTGAGATTGTGTTCCAAGGGACTGATGTACAAGGAGGCTCTGTGAATCATCCACTGCATATGCATGGATATAAGTTTTATGTCGTAGGATATGGTTTCGGAAATTATGACAATGAGACCGACCCAAAAGGGTTTAATCTTGTTGATCCTCCTCATGTCACTACATTTGGAATTCCCAAAAATGGATGGTTGGCGATCAGATTCATAGCGAATAATCCTGGTGTTTGGTTTTGGCACTGTCATATGGAGAGACACCTGACTTTGGGTATGGAAGCTGCATTCATAGTGAAGAATGGGGACACCGCTGAAACTAGCATACTCGAACCCCCAGCTTACATGCCTTCCTGTAATGTTCCATTGGCTTCTCGTTTCAAAAATTCTGATGTGTTTGTTGAAAAGAAAATAGATCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00053 ko01100 map00053 map01100
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

596

Amino Acids

67.36

Weight (kDa)

5.37

Isoelectric Point (pI)

31.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cu-oxidase_3 PF07732 38 - 149 1.9e-37 Multicopper oxidase
Cu-oxidase PF00394 163 - 314 1.2e-32 Multicopper oxidase
Cu-oxidase_2 PF07731 422 - 558 1.5e-38 Multicopper oxidase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000320)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g12640 FvH4_2g18980 FvH4_2g18990 FvH4_2g40270 FvH4_2g40270 FvH4_4g22740 FvH4_4g22740 FvH4_5g35760 FvH4_5g35760 FvH4_5g35760 FvH4_5g35760 FvH4_5g35760 FvH4_5g35760 FvH4_5g35760 FvH4_5g35760 FvH4_5g36460 FvH4_5g36460 FvH4_6g39950 FvH4_6g39970 FvH4_6g39983 FvH4_6g39990
rosa_chinensis RchiOBHm_Chr2g0154481 RchiOBHm_Chr2g0154511 RchiOBHm_Chr4g0428961 RchiOBHm_Chr6g0252431 RchiOBHm_Chr6g0273301 RchiOBHm_Chr6g0273631 RchiOBHm_Chr6g0273651 RchiOBHm_Chr6g0284751 RchiOBHm_Chr6g0284761 RchiOBHm_Chr6g0300491 RchiOBHm_Chr7g0237421 RchiOBHm_Chr7g0238581
rosa_laevigata RLG00000000906 RLG00000001006 RLG00000007110 RLG00000011333 RLG00000012721 RLG00000013605 RLG00000013626 RLG00000020780 RLG00000020782 RLG00000020783 RLG00000020786
rosa_multiflora Rmu_co8321753.1_g000001 Rmu_sc0000239.1_g000020 Rmu_sc0000686.1_g000001 Rmu_sc0000686.1_g000003 Rmu_sc0000686.1_g000005 Rmu_sc0001476.1_g000011 Rmu_sc0002231.1_g000002 Rmu_sc0002231.1_g000016 Rmu_sc0002690.1_g000003 Rmu_sc0002717.1_g000015 Rmu_sc0002923.1_g000026 Rmu_sc0003808.1_g000016 Rmu_sc0006475.1_g000008 Rmu_sc0006475.1_g000012 Rmu_sc0006475.1_g000018 Rmu_sc0014815.1_g000004 Rmu_sc0015313.1_g000012
rosa_roxburghii Rroxscaffold_2G00094270 Rroxscaffold_2G00094290 Rroxscaffold_3G00222950 Rroxscaffold_3G00224250 Rroxscaffold_5G00370630 Rroxscaffold_7G00167740 Rroxscaffold_7G00184410 Rroxscaffold_7G00195190 Rroxscaffold_7G00195460
rosa_rugosa Rorug02G0444600 Rorug04G0231100 Rorug04G0231200 Rorug06G0074000 Rorug06G0076800 Rorug06G0166400 Rorug06G0166500 Rorug06G0166600 Rorug06G0296400 Rorug07G0303000
rosa_samantha Rh2CG494600 Rh2CG494700 Rh2DG530800 Rh2DG530900 Rh2DG531000 Rh4AG287500 Rh4AG403800 Rh4BG293400 Rh4BG415100 Rh4DG290500 Rh6AG191000 Rh6AG191300 Rh6BG192500 Rh6BG194800 Rh6BG195200 Rh6BG279500 Rh6BG279600 Rh6DG183900 Rh6DG186600 Rh6DG274300 Rh6DG274500 Rh6DG409500 Rh7AG460600 Rh7AG468200 Rh7BG430200
rosa_wichuraiana Rw2G041820 Rw2G041830 Rw2G041840 Rw4G024930 Rw6G016470 Rw6G016720 Rw6G016730 Rw6G024020 Rw6G024030 Rw6G035790 Rw7G038130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 228, 1103, 1293, 1368
AccI GTMKAC 2 cut(s) 96, 868
AciI CCGC 2 cut(s) 1280, 1680
AclWI GGATC 2 cut(s) 618, 1517
AcoI YGGCCR 1 cut(s) 907
AcsI RAATTY 3 cut(s) 1117, 1548, 1753
AdeI CACNNNGTG 1 cut(s) 318
AfaI GTAC 6 cut(s) 90, 515, 534, 992, 1338, 1407
AfiI CCNNNNNNNGG 5 cut(s) 181, 387, 452, 1559, 1639
AflIII ACRYGT 1 cut(s) 91
AgsI TTSAA 4 cut(s) 80, 529, 1750, 1772
AjnI CCWGG 5 cut(s) 175, 331, 509, 802, 1594
AluBI AGCT 9 cut(s) 416, 1038, 1043, 1109, 1152, 1194, 1229, 1652, 1709
AluI AGCT 9 cut(s) 416, 1038, 1043, 1109, 1152, 1194, 1229, 1652, 1709
Alw26I GTCTC 3 cut(s) 251, 1487, 1621
AlwI GGATC 2 cut(s) 618, 1517
Ama87I CYCGRG 2 cut(s) 845, 1204
AoxI GGCC 2 cut(s) 429, 907
ApeKI GCWGC 4 cut(s) 601, 1007, 1152, 1652
ApoI RAATTY 3 cut(s) 1117, 1548, 1753
ArsI GACNNNNNNTTYG 2 cut(s) 78, 110
Asp700I GAANNNNTTC 1 cut(s) 122
AspLEI GCGC 1 cut(s) 852
AspS9I GGNCC 3 cut(s) 179, 299, 429
AsuC2I CCSGG 1 cut(s) 606
AsuHPI GGTGA 4 cut(s) 118, 954, 1210, 1363
AsuNHI GCTAGC 1 cut(s) 416
AvaI CYCGRG 2 cut(s) 845, 1204
AvaII GGWCC 2 cut(s) 179, 299
BalI TGGCCA 1 cut(s) 909
BauI CACGAG 1 cut(s) 411
BbvI GCAGC 4 cut(s) 613, 1019, 1139, 1639
BccI CCATC 2 cut(s) 290, 1557
BceAI ACGGC 1 cut(s) 39
BciT130I CCWGG 5 cut(s) 177, 333, 511, 804, 1596
BclI TGATCA 1 cut(s) 835
BcnI CCSGG 1 cut(s) 606
BcoDI GTCTC 3 cut(s) 251, 1487, 1621
BfaI CTAG 5 cut(s) 32, 417, 752, 879, 1689
BfmI CTRYAG 1 cut(s) 53
BfoI RGCGCY 1 cut(s) 853
BglII AGATCT 1 cut(s) 1216
BisI GCNGC 4 cut(s) 602, 1008, 1153, 1653
BlpI GCTNAGC 1 cut(s) 1039
BlsI GCNGC 4 cut(s) 603, 1009, 1154, 1654
Bme1390I CCNGG 6 cut(s) 177, 333, 511, 606, 804, 1596
Bme18I GGWCC 2 cut(s) 179, 299
BmeT110I CYCGRG 2 cut(s) 845, 1204
BmgT120I GGNCC 3 cut(s) 179, 299, 429
BmiI GGNNCC 3 cut(s) 379, 430, 760
BmrFI CCNGG 6 cut(s) 177, 333, 511, 606, 804, 1596
BmsI GCATC 1 cut(s) 571
BmtI GCTAGC 1 cut(s) 420
Bpu1102I GCTNAGC 1 cut(s) 1039
BpuEI CTTGAG 1 cut(s) 1168
BpuMI CCSGG 1 cut(s) 606
BsaBI GATNNNNATC 1 cut(s) 1166
BsaI GGTCTC 2 cut(s) 251, 1487
BsaJI CCNNGG 8 cut(s) 175, 287, 332, 605, 627, 802, 1205, 1393
BsaWI WCCGGW 1 cut(s) 181
BsaXI ACNNNNNCTCC 2 cut(s) 1404, 1434
Bsc4I CCNNNNNNNGG 5 cut(s) 181, 387, 452, 1559, 1639
Bse1I ACTGG 2 cut(s) 263, 410
Bse8I GATNNNNATC 1 cut(s) 1166
BseBI CCWGG 5 cut(s) 177, 333, 511, 804, 1596
BseDI CCNNGG 8 cut(s) 175, 287, 332, 605, 627, 802, 1205, 1393
BseGI GGATG 5 cut(s) 506, 704, 823, 1426, 1568
BseJI GATNNNNATC 1 cut(s) 1166
BseLI CCNNNNNNNGG 5 cut(s) 181, 387, 452, 1559, 1639
BseMII CTCAG 2 cut(s) 294, 1008
BseNI ACTGG 2 cut(s) 263, 410
BseRI GAGGAG 1 cut(s) 1518
BseXI GCAGC 4 cut(s) 613, 1019, 1139, 1639
BseYI CCCAGC 2 cut(s) 1194, 1705
BsgI GTGCAG 1 cut(s) 1138
BshFI GGCC 2 cut(s) 431, 909
BsiHKCI CYCGRG 2 cut(s) 845, 1204
BsiSI CCGG 2 cut(s) 182, 605
BslFI GGGAC 5 cut(s) 349, 646, 1052, 1411, 1688
BslI CCNNNNNNNGG 5 cut(s) 181, 387, 452, 1559, 1639
BsmAI GTCTC 3 cut(s) 251, 1487, 1621
BsmFI GGGAC 5 cut(s) 349, 646, 1052, 1411, 1688
BsmI GAATGC 1 cut(s) 1655
BsnI GGCC 2 cut(s) 431, 909
Bso31I GGTCTC 2 cut(s) 251, 1487
BsoBI CYCGRG 2 cut(s) 845, 1204
Bsp1407I TGTACA 1 cut(s) 1405
Bsp143I GATC 7 cut(s) 66, 610, 835, 1216, 1522, 1572, 1783
Bsp1720I GCTNAGC 1 cut(s) 1039
Bsp19I CCATGG 1 cut(s) 287
BspACI CCGC 2 cut(s) 1280, 1680
BspANI GGCC 2 cut(s) 431, 909
BspCNI CTCAG 2 cut(s) 295, 1007
BspLI GGNNCC 3 cut(s) 379, 430, 760
BspMAI CTGCAG 1 cut(s) 57
BspOI GCTAGC 1 cut(s) 420
BspPI GGATC 2 cut(s) 618, 1517
BspTNI GGTCTC 2 cut(s) 251, 1487
BsrGI TGTACA 1 cut(s) 1405
BsrI ACTGG 2 cut(s) 263, 410
BssECI CCNNGG 8 cut(s) 175, 287, 332, 605, 627, 802, 1205, 1393
BssMI GATC 7 cut(s) 66, 610, 835, 1216, 1522, 1572, 1783
BssSI CACGAG 1 cut(s) 411
BssT1I CCWWGG 3 cut(s) 287, 627, 1393
Bst2BI CACGAG 1 cut(s) 411
Bst2UI CCWGG 5 cut(s) 177, 333, 511, 804, 1596
Bst4CI ACNGT 8 cut(s) 318, 382, 451, 742, 1130, 1327, 1336, 1616
BstAUI TGTACA 1 cut(s) 1405
BstC8I GCNNGC 3 cut(s) 393, 418, 1227
BstDEI CTNAG 5 cut(s) 278, 303, 517, 994, 1039
BstDSI CCRYGG 1 cut(s) 287
BstF5I GGATG 5 cut(s) 506, 704, 823, 1426, 1568
BstH2I RGCGCY 1 cut(s) 853
BstHHI GCGC 1 cut(s) 852
BstKTI GATC 7 cut(s) 69, 613, 838, 1219, 1525, 1575, 1786
BstMAI GTCTC 3 cut(s) 251, 1487, 1621
BstMBI GATC 7 cut(s) 66, 610, 835, 1216, 1522, 1572, 1783
BstMWI GCNNNNNNNGC 1 cut(s) 1149
BstNI CCWGG 5 cut(s) 177, 333, 511, 804, 1596
BstNSI RCATGY 4 cut(s) 95, 154, 395, 1717
BstSCI CCNGG 6 cut(s) 175, 331, 509, 604, 802, 1594
BstSFI CTRYAG 1 cut(s) 53
BstV1I GCAGC 4 cut(s) 613, 1019, 1139, 1639
BstX2I RGATCY 2 cut(s) 610, 1216
BstYI RGATCY 2 cut(s) 610, 1216
BsuRI GGCC 2 cut(s) 431, 909
BtgI CCRYGG 1 cut(s) 287
BtsCI GGATG 5 cut(s) 506, 704, 823, 1426, 1568
BtsI GCAGTG 1 cut(s) 1430
BtsIMutI CAGTG 4 cut(s) 256, 323, 1430, 1612
Cac8I GCNNGC 3 cut(s) 393, 418, 1227
CfoI GCGC 1 cut(s) 852
Cfr13I GGNCC 3 cut(s) 179, 299, 429
Csp6I GTAC 6 cut(s) 89, 514, 533, 991, 1337, 1406
CviQI GTAC 6 cut(s) 89, 514, 533, 991, 1337, 1406
DdeI CTNAG 5 cut(s) 278, 303, 517, 994, 1039
DpnI GATC 7 cut(s) 68, 612, 837, 1218, 1524, 1574, 1785
DpnII GATC 7 cut(s) 66, 610, 835, 1216, 1522, 1572, 1783
DraIII CACNNNGTG 1 cut(s) 318
EaeI YGGCCR 1 cut(s) 907
Eco130I CCWWGG 3 cut(s) 287, 627, 1393
Eco31I GGTCTC 2 cut(s) 251, 1487
Eco32I GATATC 1 cut(s) 40
Eco47I GGWCC 2 cut(s) 179, 299
Eco88I CYCGRG 2 cut(s) 845, 1204
EcoRI GAATTC 2 cut(s) 1117, 1548
EcoRII CCWGG 5 cut(s) 175, 331, 509, 802, 1594
EcoRV GATATC 1 cut(s) 40
EcoT14I CCWWGG 3 cut(s) 287, 627, 1393
EcoT22I ATGCAT 2 cut(s) 586, 1443
ErhI CCWWGG 3 cut(s) 287, 627, 1393
FalI AAGNNNNNCTT 2 cut(s) 1027, 1059
FaqI GGGAC 5 cut(s) 349, 646, 1052, 1411, 1688
FauNDI CATATG 2 cut(s) 1437, 1620
FbaI TGATCA 1 cut(s) 835
FblI GTMKAC 2 cut(s) 96, 868
Fnu4HI GCNGC 4 cut(s) 602, 1008, 1153, 1653
FokI GGATG 5 cut(s) 493, 691, 830, 1413, 1575
Fsp4HI GCNGC 4 cut(s) 602, 1008, 1153, 1653
FspBI CTAG 5 cut(s) 32, 417, 752, 879, 1689
GlaI GCGC 1 cut(s) 851
GluI GCNGC 4 cut(s) 602, 1008, 1153, 1653
GsaI CCCAGC 2 cut(s) 1198, 1709
HaeII RGCGCY 1 cut(s) 853
HaeIII GGCC 2 cut(s) 431, 909
HapII CCGG 2 cut(s) 182, 605
HhaI GCGC 1 cut(s) 852
Hin6I GCGC 1 cut(s) 850
HinP1I GCGC 1 cut(s) 850
HincII GTYRAC 1 cut(s) 1332
HindII GTYRAC 1 cut(s) 1332
HindIII AAGCTT 2 cut(s) 1036, 1227
HinfI GANTC 5 cut(s) 701, 1136, 1373, 1423, 1578
HpaI GTTAAC 1 cut(s) 1332
HpaII CCGG 2 cut(s) 182, 605
HphI GGTGA 4 cut(s) 118, 954, 1210, 1363
Hpy166II GTNNAC 6 cut(s) 97, 163, 869, 1201, 1255, 1332
Hpy188I TCNGA 7 cut(s) 295, 580, 749, 997, 1478, 1577, 1759
Hpy188III TCNNGA 4 cut(s) 61, 302, 1147, 1271
Hpy8I GTNNAC 6 cut(s) 97, 163, 869, 1201, 1255, 1332
HpyAV CCTTC 3 cut(s) 368, 1348, 1728
HpyCH4III ACNGT 8 cut(s) 318, 382, 451, 742, 1130, 1327, 1336, 1616
HpyF10VI GCNNNNNNNGC 1 cut(s) 1149
HpyF3I CTNAG 5 cut(s) 278, 303, 517, 994, 1039
HspAI GCGC 1 cut(s) 850
Ksp22I TGATCA 1 cut(s) 835
KspAI GTTAAC 1 cut(s) 1332
Kzo9I GATC 7 cut(s) 66, 610, 835, 1216, 1522, 1572, 1783
LmnI GCTCC 2 cut(s) 629, 758
Lsp1109I GCAGC 4 cut(s) 613, 1019, 1139, 1639
LweI GCATC 1 cut(s) 571
MaeI CTAG 5 cut(s) 32, 417, 752, 879, 1689
MaeIII GTNAC 3 cut(s) 401, 916, 1534
MalI GATC 7 cut(s) 68, 612, 837, 1218, 1524, 1574, 1785
MboI GATC 7 cut(s) 66, 610, 835, 1216, 1522, 1572, 1783
MboII GAAGA 6 cut(s) 383, 497, 665, 668, 1145, 1678
MflI RGATCY 2 cut(s) 610, 1216
MlsI TGGCCA 1 cut(s) 909
MluCI AATT 7 cut(s) 340, 902, 1029, 1117, 1480, 1548, 1753
MluNI TGGCCA 1 cut(s) 909
MlyI GAGTC 1 cut(s) 1382
MmeI TCCRAC 4 cut(s) 352, 433, 727, 980
Mox20I TGGCCA 1 cut(s) 909
Mph1103I ATGCAT 2 cut(s) 586, 1443
MroXI GAANNNNTTC 1 cut(s) 122
MscI TGGCCA 1 cut(s) 909
MseI TTAA 6 cut(s) 110, 1013, 1032, 1331, 1361, 1512
MslI CAYNNNNRTG 2 cut(s) 261, 1440
Msp20I TGGCCA 1 cut(s) 909
MspA1I CMGCKG 1 cut(s) 1682
MspI CCGG 2 cut(s) 182, 605
MspR9I CCNGG 6 cut(s) 177, 333, 511, 606, 804, 1596
Mva1269I GAATGC 1 cut(s) 1655
MvaI CCWGG 5 cut(s) 177, 333, 511, 804, 1596
MwoI GCNNNNNNNGC 1 cut(s) 1149
NciI CCSGG 1 cut(s) 606
NcoI CCATGG 1 cut(s) 287
NdeI CATATG 2 cut(s) 1437, 1620
NdeII GATC 7 cut(s) 66, 610, 835, 1216, 1522, 1572, 1783
NheI GCTAGC 1 cut(s) 416
NlaIV GGNNCC 3 cut(s) 379, 430, 760
NmuCI GTSAC 2 cut(s) 401, 1534
NsiI ATGCAT 2 cut(s) 586, 1443
NspI RCATGY 4 cut(s) 95, 154, 395, 1717
PaeI GCATGC 1 cut(s) 395
PciI ACATGT 1 cut(s) 91
PcsI WCGNNNNNNNCGW 1 cut(s) 1176
PctI GAATGC 1 cut(s) 1655
PdmI GAANNNNTTC 1 cut(s) 122
PfeI GAWTC 4 cut(s) 701, 1136, 1423, 1578
PkrI GCNGC 4 cut(s) 603, 1009, 1154, 1654
PleI GAGTC 1 cut(s) 1381
PpsI GAGTC 1 cut(s) 1381
PscI ACATGT 1 cut(s) 91
PsiI TTATAA 4 cut(s) 228, 1103, 1293, 1368
Psp6I CCWGG 5 cut(s) 175, 331, 509, 802, 1594
PspFI CCCAGC 2 cut(s) 1194, 1705
PspGI CCWGG 5 cut(s) 175, 331, 509, 802, 1594
PspN4I GGNNCC 3 cut(s) 379, 430, 760
PspPI GGNCC 3 cut(s) 179, 299, 429
PstI CTGCAG 1 cut(s) 57
PsuI RGATCY 2 cut(s) 610, 1216
RsaI GTAC 6 cut(s) 90, 515, 534, 992, 1338, 1407
RsaNI GTAC 6 cut(s) 89, 514, 533, 991, 1337, 1406
RseI CAYNNNNRTG 2 cut(s) 261, 1440
SaqAI TTAA 6 cut(s) 110, 1013, 1032, 1331, 1361, 1512
SatI GCNGC 4 cut(s) 602, 1008, 1153, 1653
Sau3AI GATC 7 cut(s) 66, 610, 835, 1216, 1522, 1572, 1783
Sau96I GGNCC 3 cut(s) 179, 299, 429
SchI GAGTC 1 cut(s) 1382
ScrFI CCNGG 6 cut(s) 177, 333, 511, 606, 804, 1596
SfaNI GCATC 1 cut(s) 571
SfcI CTRYAG 1 cut(s) 53
SinI GGWCC 2 cut(s) 179, 299
SmiMI CAYNNNNRTG 2 cut(s) 261, 1440
SmlI CTYRAG 1 cut(s) 1147
SmoI CTYRAG 1 cut(s) 1147
SphI GCATGC 1 cut(s) 395
Sse9I AATT 7 cut(s) 340, 902, 1029, 1117, 1480, 1548, 1753
SsiI CCGC 2 cut(s) 1280, 1680
SspMI CTAG 5 cut(s) 32, 417, 752, 879, 1689
StyD4I CCNGG 6 cut(s) 175, 331, 509, 604, 802, 1594
StyI CCWWGG 3 cut(s) 287, 627, 1393
TaaI ACNGT 8 cut(s) 318, 382, 451, 742, 1130, 1327, 1336, 1616
TaqI TCGA 2 cut(s) 1316, 1698
TaqII GACCGA 1 cut(s) 1511
TasI AATT 7 cut(s) 340, 902, 1029, 1117, 1480, 1548, 1753
TatI WGTACW 4 cut(s) 88, 990, 1336, 1405
TfiI GAWTC 4 cut(s) 701, 1136, 1423, 1578
Tru1I TTAA 6 cut(s) 110, 1013, 1032, 1331, 1361, 1512
Tru9I TTAA 6 cut(s) 110, 1013, 1032, 1331, 1361, 1512
TscAI CASTG 4 cut(s) 263, 323, 1437, 1619
TseFI GTSAC 2 cut(s) 401, 1534
TseI GCWGC 4 cut(s) 601, 1007, 1152, 1652
Tsp45I GTSAC 2 cut(s) 401, 1534
TspDTI ATGAA 9 cut(s) 17, 185, 413, 498, 566, 693, 1130, 1570, 1648
TspGWI ACGGA 2 cut(s) 383, 771
TspRI CASTG 4 cut(s) 263, 323, 1437, 1619
VpaK11BI GGWCC 2 cut(s) 179, 299
XapI RAATTY 3 cut(s) 1117, 1548, 1753
XceI RCATGY 4 cut(s) 95, 154, 395, 1717
XmiI GTMKAC 2 cut(s) 96, 868
XmnI GAANNNNTTC 1 cut(s) 122
XspI CTAG 5 cut(s) 32, 417, 752, 879, 1689
Zsp2I ATGCAT 2 cut(s) 586, 1443
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.