Lignin degradation and detoxification of lignin-derived products
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 3 cut(s) | 1103, 1293, 1368 |
| AccB1I | GGYRCC | 1 cut(s) | 425 |
| AccI | GTMKAC | 1 cut(s) | 865 |
| AccII | CGCG | 1 cut(s) | 409 |
| AciI | CCGC | 2 cut(s) | 1280, 1680 |
| AclWI | GGATC | 2 cut(s) | 615, 1517 |
| AcoI | YGGCCR | 1 cut(s) | 595 |
| AcsI | RAATTY | 2 cut(s) | 899, 1753 |
| AcyI | GRCGYC | 1 cut(s) | 426 |
| AdeI | CACNNNGTG | 1 cut(s) | 315 |
| AfaI | GTAC | 4 cut(s) | 87, 512, 992, 1338 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 178, 449, 604, 1559 |
| AgsI | TTSAA | 3 cut(s) | 77, 526, 1772 |
| AjnI | CCWGG | 5 cut(s) | 172, 328, 506, 799, 1594 |
| AluBI | AGCT | 7 cut(s) | 1038, 1109, 1152, 1194, 1229, 1652, 1709 |
| AluI | AGCT | 7 cut(s) | 1038, 1109, 1152, 1194, 1229, 1652, 1709 |
| Alw21I | GWGCWC | 1 cut(s) | 1214 |
| Alw26I | GTCTC | 3 cut(s) | 248, 623, 1487 |
| AlwI | GGATC | 2 cut(s) | 615, 1517 |
| AoxI | GGCC | 2 cut(s) | 176, 595 |
| ApeKI | GCWGC | 3 cut(s) | 1004, 1152, 1652 |
| ApoI | RAATTY | 2 cut(s) | 899, 1753 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 119 |
| AspLEI | GCGC | 2 cut(s) | 428, 849 |
| AspS9I | GGNCC | 2 cut(s) | 176, 296 |
| AsuC2I | CCSGG | 2 cut(s) | 127, 603 |
| AsuHPI | GGTGA | 3 cut(s) | 115, 1210, 1363 |
| AvaII | GGWCC | 1 cut(s) | 296 |
| BalI | TGGCCA | 1 cut(s) | 597 |
| BanI | GGYRCC | 1 cut(s) | 425 |
| Bbv12I | GWGCWC | 1 cut(s) | 1214 |
| BbvI | GCAGC | 3 cut(s) | 1016, 1139, 1639 |
| BccI | CCATC | 4 cut(s) | 13, 287, 1121, 1557 |
| BciT130I | CCWGG | 5 cut(s) | 174, 330, 508, 801, 1596 |
| BciVI | GTATCC | 1 cut(s) | 196 |
| BcnI | CCSGG | 2 cut(s) | 127, 603 |
| BcoDI | GTCTC | 3 cut(s) | 248, 623, 1487 |
| BfaI | CTAG | 3 cut(s) | 749, 876, 1689 |
| BfmI | CTRYAG | 2 cut(s) | 50, 1234 |
| BfoI | RGCGCY | 2 cut(s) | 429, 850 |
| BfuI | GTATCC | 1 cut(s) | 196 |
| BglII | AGATCT | 1 cut(s) | 1216 |
| BisI | GCNGC | 3 cut(s) | 1005, 1153, 1653 |
| BlpI | GCTNAGC | 1 cut(s) | 1039 |
| BlsI | GCNGC | 3 cut(s) | 1006, 1154, 1654 |
| Bme1390I | CCNGG | 7 cut(s) | 127, 174, 330, 508, 603, 801, 1596 |
| Bme18I | GGWCC | 1 cut(s) | 296 |
| BmgT120I | GGNCC | 2 cut(s) | 176, 296 |
| BmiI | GGNNCC | 2 cut(s) | 427, 757 |
| BmrFI | CCNGG | 7 cut(s) | 127, 174, 330, 508, 603, 801, 1596 |
| BmsI | GCATC | 3 cut(s) | 568, 688, 1016 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1039 |
| BpuEI | CTTGAG | 1 cut(s) | 1168 |
| BpuMI | CCSGG | 2 cut(s) | 127, 603 |
| BsaHI | GRCGYC | 1 cut(s) | 426 |
| BsaI | GGTCTC | 1 cut(s) | 248 |
| BsaJI | CCNNGG | 8 cut(s) | 172, 173, 284, 329, 602, 799, 1205, 1393 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1404, 1434 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 178, 449, 604, 1559 |
| Bse1I | ACTGG | 3 cut(s) | 260, 407, 1786 |
| BseBI | CCWGG | 5 cut(s) | 174, 330, 508, 801, 1596 |
| BseDI | CCNNGG | 8 cut(s) | 172, 173, 284, 329, 602, 799, 1205, 1393 |
| BseGI | GGATG | 5 cut(s) | 503, 786, 820, 1426, 1568 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 178, 449, 604, 1559 |
| BseMII | CTCAG | 1 cut(s) | 291 |
| BseNI | ACTGG | 3 cut(s) | 260, 407, 1786 |
| BseRI | GAGGAG | 2 cut(s) | 954, 1518 |
| BseXI | GCAGC | 3 cut(s) | 1016, 1139, 1639 |
| BseYI | CCCAGC | 2 cut(s) | 1194, 1705 |
| Bsh1236I | CGCG | 1 cut(s) | 409 |
| BshFI | GGCC | 2 cut(s) | 178, 597 |
| BshNI | GGYRCC | 1 cut(s) | 425 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1214 |
| BsiSI | CCGG | 3 cut(s) | 126, 179, 602 |
| BslFI | GGGAC | 5 cut(s) | 346, 1052, 1357, 1411, 1688 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 178, 449, 604, 1559 |
| BsmAI | GTCTC | 3 cut(s) | 248, 623, 1487 |
| BsmFI | GGGAC | 5 cut(s) | 346, 1052, 1357, 1411, 1688 |
| BsmI | GAATGC | 1 cut(s) | 1655 |
| BsnI | GGCC | 2 cut(s) | 178, 597 |
| Bso31I | GGTCTC | 1 cut(s) | 248 |
| Bsp1286I | GDGCHC | 1 cut(s) | 1214 |
| Bsp143I | GATC | 5 cut(s) | 63, 607, 832, 1216, 1522 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1039 |
| Bsp19I | CCATGG | 1 cut(s) | 284 |
| BspACI | CCGC | 2 cut(s) | 1280, 1680 |
| BspANI | GGCC | 2 cut(s) | 178, 597 |
| BspCNI | CTCAG | 1 cut(s) | 292 |
| BspFNI | CGCG | 1 cut(s) | 409 |
| BspLI | GGNNCC | 2 cut(s) | 427, 757 |
| BspMAI | CTGCAG | 1 cut(s) | 54 |
| BspPI | GGATC | 2 cut(s) | 615, 1517 |
| BspT107I | GGYRCC | 1 cut(s) | 425 |
| BspTNI | GGTCTC | 1 cut(s) | 248 |
| BsrI | ACTGG | 3 cut(s) | 260, 407, 1786 |
| BssECI | CCNNGG | 8 cut(s) | 172, 173, 284, 329, 602, 799, 1205, 1393 |
| BssMI | GATC | 5 cut(s) | 63, 607, 832, 1216, 1522 |
| BssNI | GRCGYC | 1 cut(s) | 426 |
| BssT1I | CCWWGG | 3 cut(s) | 284, 1205, 1393 |
| Bst2UI | CCWGG | 5 cut(s) | 174, 330, 508, 801, 1596 |
| Bst4CI | ACNGT | 8 cut(s) | 208, 315, 379, 448, 739, 1130, 1327, 1336 |
| BstACI | GRCGYC | 1 cut(s) | 426 |
| BstC8I | GCNNGC | 6 cut(s) | 390, 411, 415, 599, 1227, 1569 |
| BstDEI | CTNAG | 3 cut(s) | 300, 994, 1039 |
| BstDSI | CCRYGG | 1 cut(s) | 284 |
| BstF5I | GGATG | 5 cut(s) | 503, 786, 820, 1426, 1568 |
| BstFNI | CGCG | 1 cut(s) | 409 |
| BstH2I | RGCGCY | 2 cut(s) | 429, 850 |
| BstHHI | GCGC | 2 cut(s) | 428, 849 |
| BstKTI | GATC | 5 cut(s) | 66, 610, 835, 1219, 1525 |
| BstMAI | GTCTC | 3 cut(s) | 248, 623, 1487 |
| BstMBI | GATC | 5 cut(s) | 63, 607, 832, 1216, 1522 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 423, 1149 |
| BstNI | CCWGG | 5 cut(s) | 174, 330, 508, 801, 1596 |
| BstNSI | RCATGY | 3 cut(s) | 151, 392, 1717 |
| BstSCI | CCNGG | 7 cut(s) | 125, 172, 328, 506, 601, 799, 1594 |
| BstSFI | CTRYAG | 2 cut(s) | 50, 1234 |
| BstUI | CGCG | 1 cut(s) | 409 |
| BstV1I | GCAGC | 3 cut(s) | 1016, 1139, 1639 |
| BstX2I | RGATCY | 2 cut(s) | 607, 1216 |
| BstYI | RGATCY | 2 cut(s) | 607, 1216 |
| BsuI | GTATCC | 1 cut(s) | 196 |
| BsuRI | GGCC | 2 cut(s) | 178, 597 |
| BtgI | CCRYGG | 1 cut(s) | 284 |
| BtsCI | GGATG | 5 cut(s) | 503, 786, 820, 1426, 1568 |
| BtsI | GCAGTG | 1 cut(s) | 1430 |
| BtsIMutI | CAGTG | 4 cut(s) | 253, 320, 735, 1430 |
| Cac8I | GCNNGC | 6 cut(s) | 390, 411, 415, 599, 1227, 1569 |
| CfoI | GCGC | 2 cut(s) | 428, 849 |
| Cfr13I | GGNCC | 2 cut(s) | 176, 296 |
| CseI | GACGC | 1 cut(s) | 415 |
| Csp6I | GTAC | 4 cut(s) | 86, 511, 991, 1337 |
| CviQI | GTAC | 4 cut(s) | 86, 511, 991, 1337 |
| DdeI | CTNAG | 3 cut(s) | 300, 994, 1039 |
| DinI | GGCGCC | 1 cut(s) | 427 |
| DpnI | GATC | 5 cut(s) | 65, 609, 834, 1218, 1524 |
| DpnII | GATC | 5 cut(s) | 63, 607, 832, 1216, 1522 |
| DraIII | CACNNNGTG | 1 cut(s) | 315 |
| EaeI | YGGCCR | 1 cut(s) | 595 |
| Eco130I | CCWWGG | 3 cut(s) | 284, 1205, 1393 |
| Eco31I | GGTCTC | 1 cut(s) | 248 |
| Eco47I | GGWCC | 1 cut(s) | 296 |
| EcoRII | CCWGG | 5 cut(s) | 172, 328, 506, 799, 1594 |
| EcoT14I | CCWWGG | 3 cut(s) | 284, 1205, 1393 |
| EcoT22I | ATGCAT | 3 cut(s) | 583, 703, 1443 |
| EgeI | GGCGCC | 1 cut(s) | 427 |
| EheI | GGCGCC | 1 cut(s) | 427 |
| ErhI | CCWWGG | 3 cut(s) | 284, 1205, 1393 |
| FaqI | GGGAC | 5 cut(s) | 346, 1052, 1357, 1411, 1688 |
| FauNDI | CATATG | 2 cut(s) | 1437, 1620 |
| FblI | GTMKAC | 1 cut(s) | 865 |
| Fnu4HI | GCNGC | 3 cut(s) | 1005, 1153, 1653 |
| FokI | GGATG | 5 cut(s) | 490, 773, 827, 1413, 1575 |
| Fsp4HI | GCNGC | 3 cut(s) | 1005, 1153, 1653 |
| FspBI | CTAG | 3 cut(s) | 749, 876, 1689 |
| GlaI | GCGC | 2 cut(s) | 427, 848 |
| GluI | GCNGC | 3 cut(s) | 1005, 1153, 1653 |
| GsaI | CCCAGC | 2 cut(s) | 1198, 1709 |
| HaeII | RGCGCY | 2 cut(s) | 429, 850 |
| HaeIII | GGCC | 2 cut(s) | 178, 597 |
| HapII | CCGG | 3 cut(s) | 126, 179, 602 |
| HgaI | GACGC | 1 cut(s) | 415 |
| HhaI | GCGC | 2 cut(s) | 428, 849 |
| Hin1I | GRCGYC | 1 cut(s) | 426 |
| Hin6I | GCGC | 2 cut(s) | 426, 847 |
| HinP1I | GCGC | 2 cut(s) | 426, 847 |
| HincII | GTYRAC | 1 cut(s) | 1332 |
| HindII | GTYRAC | 1 cut(s) | 1332 |
| HindIII | AAGCTT | 2 cut(s) | 1036, 1227 |
| HinfI | GANTC | 4 cut(s) | 1136, 1373, 1423, 1578 |
| HpaI | GTTAAC | 1 cut(s) | 1332 |
| HpaII | CCGG | 3 cut(s) | 126, 179, 602 |
| HphI | GGTGA | 3 cut(s) | 115, 1210, 1363 |
| Hpy166II | GTNNAC | 4 cut(s) | 866, 1201, 1255, 1332 |
| Hpy188I | TCNGA | 6 cut(s) | 292, 577, 898, 1478, 1577, 1759 |
| Hpy188III | TCNNGA | 4 cut(s) | 58, 299, 1147, 1271 |
| Hpy8I | GTNNAC | 4 cut(s) | 866, 1201, 1255, 1332 |
| HpyAV | CCTTC | 5 cut(s) | 365, 548, 992, 1348, 1728 |
| HpyCH4III | ACNGT | 8 cut(s) | 208, 315, 379, 448, 739, 1130, 1327, 1336 |
| HpyCH4IV | ACGT | 1 cut(s) | 665 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 423, 1149 |
| HpyF3I | CTNAG | 3 cut(s) | 300, 994, 1039 |
| HpySE526I | ACGT | 1 cut(s) | 665 |
| Hsp92I | GRCGYC | 1 cut(s) | 426 |
| HspAI | GCGC | 2 cut(s) | 426, 847 |
| KasI | GGCGCC | 1 cut(s) | 425 |
| KspAI | GTTAAC | 1 cut(s) | 1332 |
| Kzo9I | GATC | 5 cut(s) | 63, 607, 832, 1216, 1522 |
| LmnI | GCTCC | 3 cut(s) | 626, 755, 1209 |
| Lsp1109I | GCAGC | 3 cut(s) | 1016, 1139, 1639 |
| LweI | GCATC | 3 cut(s) | 568, 688, 1016 |
| MaeI | CTAG | 3 cut(s) | 749, 876, 1689 |
| MaeII | ACGT | 1 cut(s) | 665 |
| MaeIII | GTNAC | 3 cut(s) | 398, 916, 1534 |
| MalI | GATC | 5 cut(s) | 65, 609, 834, 1218, 1524 |
| MboI | GATC | 5 cut(s) | 63, 607, 832, 1216, 1522 |
| MboII | GAAGA | 9 cut(s) | 28, 380, 494, 563, 662, 665, 703, 1145, 1678 |
| MflI | RGATCY | 2 cut(s) | 607, 1216 |
| MhlI | GDGCHC | 1 cut(s) | 1214 |
| MlsI | TGGCCA | 1 cut(s) | 597 |
| MluCI | AATT | 8 cut(s) | 272, 337, 899, 1029, 1117, 1480, 1513, 1753 |
| MluNI | TGGCCA | 1 cut(s) | 597 |
| Mly113I | GGCGCC | 1 cut(s) | 426 |
| MlyI | GAGTC | 1 cut(s) | 1382 |
| MmeI | TCCRAC | 6 cut(s) | 349, 430, 648, 724, 980, 1444 |
| Mox20I | TGGCCA | 1 cut(s) | 597 |
| Mph1103I | ATGCAT | 3 cut(s) | 583, 703, 1443 |
| MroXI | GAANNNNTTC | 1 cut(s) | 119 |
| MscI | TGGCCA | 1 cut(s) | 597 |
| MseI | TTAA | 7 cut(s) | 275, 1013, 1032, 1331, 1361, 1512, 1778 |
| MslI | CAYNNNNRTG | 5 cut(s) | 93, 258, 576, 912, 1440 |
| Msp20I | TGGCCA | 1 cut(s) | 597 |
| MspA1I | CMGCKG | 1 cut(s) | 1682 |
| MspI | CCGG | 3 cut(s) | 126, 179, 602 |
| MspR9I | CCNGG | 7 cut(s) | 127, 174, 330, 508, 603, 801, 1596 |
| Mva1269I | GAATGC | 1 cut(s) | 1655 |
| MvaI | CCWGG | 5 cut(s) | 174, 330, 508, 801, 1596 |
| MvnI | CGCG | 1 cut(s) | 409 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 423, 1149 |
| NarI | GGCGCC | 1 cut(s) | 426 |
| NciI | CCSGG | 2 cut(s) | 127, 603 |
| NcoI | CCATGG | 1 cut(s) | 284 |
| NdeI | CATATG | 2 cut(s) | 1437, 1620 |
| NdeII | GATC | 5 cut(s) | 63, 607, 832, 1216, 1522 |
| NlaIV | GGNNCC | 2 cut(s) | 427, 757 |
| NmuCI | GTSAC | 2 cut(s) | 398, 1534 |
| NsiI | ATGCAT | 3 cut(s) | 583, 703, 1443 |
| NspI | RCATGY | 3 cut(s) | 151, 392, 1717 |
| PaeI | GCATGC | 1 cut(s) | 392 |
| PasI | CCCWGGG | 1 cut(s) | 173 |
| PctI | GAATGC | 1 cut(s) | 1655 |
| PdmI | GAANNNNTTC | 1 cut(s) | 119 |
| PfeI | GAWTC | 3 cut(s) | 1136, 1423, 1578 |
| PflFI | GACNNNGTC | 1 cut(s) | 908 |
| PkrI | GCNGC | 3 cut(s) | 1006, 1154, 1654 |
| PleI | GAGTC | 1 cut(s) | 1381 |
| PluTI | GGCGCC | 1 cut(s) | 429 |
| PpsI | GAGTC | 1 cut(s) | 1381 |
| PsiI | TTATAA | 3 cut(s) | 1103, 1293, 1368 |
| Psp6I | CCWGG | 5 cut(s) | 172, 328, 506, 799, 1594 |
| PspFI | CCCAGC | 2 cut(s) | 1194, 1705 |
| PspGI | CCWGG | 5 cut(s) | 172, 328, 506, 799, 1594 |
| PspN4I | GGNNCC | 2 cut(s) | 427, 757 |
| PspPI | GGNCC | 2 cut(s) | 176, 296 |
| PstI | CTGCAG | 1 cut(s) | 54 |
| PsuI | RGATCY | 2 cut(s) | 607, 1216 |
| PsyI | GACNNNGTC | 1 cut(s) | 908 |
| RsaI | GTAC | 4 cut(s) | 87, 512, 992, 1338 |
| RsaNI | GTAC | 4 cut(s) | 86, 511, 991, 1337 |
| RseI | CAYNNNNRTG | 5 cut(s) | 93, 258, 576, 912, 1440 |
| SaqAI | TTAA | 7 cut(s) | 275, 1013, 1032, 1331, 1361, 1512, 1778 |
| SatI | GCNGC | 3 cut(s) | 1005, 1153, 1653 |
| Sau3AI | GATC | 5 cut(s) | 63, 607, 832, 1216, 1522 |
| Sau96I | GGNCC | 2 cut(s) | 176, 296 |
| SchI | GAGTC | 1 cut(s) | 1382 |
| ScrFI | CCNGG | 7 cut(s) | 127, 174, 330, 508, 603, 801, 1596 |
| SduI | GDGCHC | 1 cut(s) | 1214 |
| SfaNI | GCATC | 3 cut(s) | 568, 688, 1016 |
| SfcI | CTRYAG | 2 cut(s) | 50, 1234 |
| SfoI | GGCGCC | 1 cut(s) | 427 |
| SinI | GGWCC | 1 cut(s) | 296 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 93, 258, 576, 912, 1440 |
| SmlI | CTYRAG | 1 cut(s) | 1147 |
| SmoI | CTYRAG | 1 cut(s) | 1147 |
| SphI | GCATGC | 1 cut(s) | 392 |
| Sse9I | AATT | 8 cut(s) | 272, 337, 899, 1029, 1117, 1480, 1513, 1753 |
| SsiI | CCGC | 2 cut(s) | 1280, 1680 |
| SspDI | GGCGCC | 1 cut(s) | 425 |
| SspMI | CTAG | 3 cut(s) | 749, 876, 1689 |
| StyD4I | CCNGG | 7 cut(s) | 125, 172, 328, 506, 601, 799, 1594 |
| StyI | CCWWGG | 3 cut(s) | 284, 1205, 1393 |
| TaaI | ACNGT | 8 cut(s) | 208, 315, 379, 448, 739, 1130, 1327, 1336 |
| TaiI | ACGT | 1 cut(s) | 668 |
| TaqI | TCGA | 4 cut(s) | 643, 1316, 1698, 1749 |
| TasI | AATT | 8 cut(s) | 272, 337, 899, 1029, 1117, 1480, 1513, 1753 |
| TatI | WGTACW | 3 cut(s) | 85, 990, 1336 |
| TfiI | GAWTC | 3 cut(s) | 1136, 1423, 1578 |
| Tru1I | TTAA | 7 cut(s) | 275, 1013, 1032, 1331, 1361, 1512, 1778 |
| Tru9I | TTAA | 7 cut(s) | 275, 1013, 1032, 1331, 1361, 1512, 1778 |
| TscAI | CASTG | 4 cut(s) | 260, 320, 742, 1437 |
| TseFI | GTSAC | 2 cut(s) | 398, 1534 |
| TseI | GCWGC | 3 cut(s) | 1004, 1152, 1652 |
| Tsp45I | GTSAC | 2 cut(s) | 398, 1534 |
| TspDTI | ATGAA | 7 cut(s) | 17, 182, 212, 410, 495, 1570, 1648 |
| TspGWI | ACGGA | 2 cut(s) | 380, 768 |
| TspRI | CASTG | 4 cut(s) | 260, 320, 742, 1437 |
| Tth111I | GACNNNGTC | 1 cut(s) | 908 |
| VpaK11BI | GGWCC | 1 cut(s) | 296 |
| XapI | RAATTY | 2 cut(s) | 899, 1753 |
| XceI | RCATGY | 3 cut(s) | 151, 392, 1717 |
| XmiI | GTMKAC | 1 cut(s) | 865 |
| XmnI | GAANNNNTTC | 1 cut(s) | 119 |
| XspI | CTAG | 3 cut(s) | 749, 876, 1689 |
| Zsp2I | ATGCAT | 3 cut(s) | 583, 703, 1443 |