Rmu_sc0006475.1_g000018

Lignin degradation and detoxification of lignin-derived products

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006475.1
OG0000320
Physical Location & Seq
Reverse (-)
87370 .. 90543
3174 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0006475.1_g000018.1.cds

Sequence Viewer

Length: 2127 bp
atgaaatccatgaagagtactactagtccagtactgatgtcgacacagtttctggtgttcgctgctcttattatcatcctaccgctcttcatctctctggctcgagcagaagttcattactacgattttatagttaaggaggtaaacttcactagattgtgtgaatcaaagctcatgttagtagtaaacgagagctatccaggtccagcaatacgagctcgcaaaggggatacaatttatgttaatgtttacaatcaaggattttatggtttcaccattcactggcaagtcccatcacacgacctcccctatctgtttagtttgaccacagaagaaggaactctgtggtggcatgctcatagtgactggactagggcaagcgttcacggtgccattgtcatcttgcctgctcttggaacaacatttccatttcctgagcctgatgaagaggagatcattgtgttcggatcttggtatttgggaaatttgaaagaacaggttgacgagtgtctggaccctgcaaccaactctaacttacccatagcagctgatacaacgtatcgtcgtaaggtggactatggcaagacatatcttcttcggatagtgaacgcaaacatcgatgtagagttttactttgccattgctgaacacaacctcactgtggttggcttggacggctcctataccaaacccatcaacacaggctatatagtgatagcccctggacaaacaatggacgtgttgctcaacacaaattcggctctggggagctattacatggtcggaagagaatacataagtgctacagttgacacagtatctgactctgcgaatgatgctattgcaatccttgaatataatggtaactataccacttccgaagctcctttatttccccaacaaattcccagttccttatcccaaacagatgcattcaacttctacggaaatattagaagcttagccaccccagagtatcctataaatgtcccaaaagagagcgacataactaccaaaatgtacataacagcttccgccaacgctctttattgtactccagatttaggtccctcatgcgtaaacatatcttttgctgcaagcgtcaataatatcagttgggtcaatccgagagaatctatcttgcaagcctactacaggaacataagcgagccggtttatgaaacagattttccagacgaaccacctacttattttaacttcactgaaatagcgatgacactagatcgagtgttgacagtccaagggacaaaggtcaaggtgctagaatataatgaaacagttgagatggtgtttcaaggaactgatgtgatgggaggttccgtgaatcatccaatgcacttgcatggacatagcttttatgtgcttggatttggctttggggatttcttggccgaaagagactcaaaaggttataatttgattgatcctccttacgtaacaacgtttataactcctaaaaacggatggttaaccataagatttgtagcgaataatccaggtgtgtggttttggcattgtcacatggagagacacttgacatggggtatggagtctgcttttatagtgaagaacgggggcacaactgagactaatatgctcggtcctccagctatcttgccttcctgtgatgttccattggattctgctacaatcctaagtcacgcagaattgattaataatcaaatagaaacgcgcttgcacgcgcctgagaaagaagaaaagttcctgctccctgtccagcggcgagcccttacggcgccgtcgtcggacgggtctttttccgactcgggtgggtttgatgtgaagctagggtcctcgatggttgtctatgagtggagggaggagacgattgcggcggttatcaagccatggttccttcttcgctggcctgattggcttggaggcgtgggatcggaggctgcctggtggaggcaagcttgtgtcggggcggtctggatcggatcagaactatctgatctggattgggtcccatgtttcggatctggggaatggtttgggcggaagggagcgcgtttctccatggctcgacggggaacgtggtacgacgatgacggcggtccggcgaagggggttgtgcagcgctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00053 ko01100 map00053 map01100
Pfam Domains
Protein Families
Cu-oxidase Cu-oxidase_2 Cu-oxidase_3

Protein Analysis

708

Amino Acids

79.18

Weight (kDa)

4.94

Isoelectric Point (pI)

41.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000320)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g12640 FvH4_2g18980 FvH4_2g18990 FvH4_2g40270 FvH4_2g40270 FvH4_4g22740 FvH4_4g22740 FvH4_5g35760 FvH4_5g35760 FvH4_5g35760 FvH4_5g35760 FvH4_5g35760 FvH4_5g35760 FvH4_5g35760 FvH4_5g35760 FvH4_5g36460 FvH4_5g36460 FvH4_6g39950 FvH4_6g39970 FvH4_6g39983 FvH4_6g39990
rosa_chinensis RchiOBHm_Chr2g0154481 RchiOBHm_Chr2g0154511 RchiOBHm_Chr4g0428961 RchiOBHm_Chr6g0252431 RchiOBHm_Chr6g0273301 RchiOBHm_Chr6g0273631 RchiOBHm_Chr6g0273651 RchiOBHm_Chr6g0284751 RchiOBHm_Chr6g0284761 RchiOBHm_Chr6g0300491 RchiOBHm_Chr7g0237421 RchiOBHm_Chr7g0238581
rosa_laevigata RLG00000000906 RLG00000001006 RLG00000007110 RLG00000011333 RLG00000012721 RLG00000013605 RLG00000013626 RLG00000020780 RLG00000020782 RLG00000020783 RLG00000020786
rosa_multiflora Rmu_co8321753.1_g000001 Rmu_sc0000239.1_g000020 Rmu_sc0000686.1_g000001 Rmu_sc0000686.1_g000003 Rmu_sc0000686.1_g000005 Rmu_sc0001476.1_g000011 Rmu_sc0002231.1_g000002 Rmu_sc0002231.1_g000016 Rmu_sc0002690.1_g000003 Rmu_sc0002717.1_g000015 Rmu_sc0002923.1_g000026 Rmu_sc0003808.1_g000016 Rmu_sc0006475.1_g000008 Rmu_sc0006475.1_g000012 Rmu_sc0006475.1_g000018 Rmu_sc0014815.1_g000004 Rmu_sc0015313.1_g000012
rosa_roxburghii Rroxscaffold_2G00094270 Rroxscaffold_2G00094290 Rroxscaffold_3G00222950 Rroxscaffold_3G00224250 Rroxscaffold_5G00370630 Rroxscaffold_7G00167740 Rroxscaffold_7G00184410 Rroxscaffold_7G00195190 Rroxscaffold_7G00195460
rosa_rugosa Rorug02G0444600 Rorug04G0231100 Rorug04G0231200 Rorug06G0074000 Rorug06G0076800 Rorug06G0166400 Rorug06G0166500 Rorug06G0166600 Rorug06G0296400 Rorug07G0303000
rosa_samantha Rh2CG494600 Rh2CG494700 Rh2DG530800 Rh2DG530900 Rh2DG531000 Rh4AG287500 Rh4AG403800 Rh4BG293400 Rh4BG415100 Rh4DG290500 Rh6AG191000 Rh6AG191300 Rh6BG192500 Rh6BG194800 Rh6BG195200 Rh6BG279500 Rh6BG279600 Rh6DG183900 Rh6DG186600 Rh6DG274300 Rh6DG274500 Rh6DG409500 Rh7AG460600 Rh7AG468200 Rh7BG430200
rosa_wichuraiana Rw2G041820 Rw2G041830 Rw2G041840 Rw4G024930 Rw6G016470 Rw6G016720 Rw6G016730 Rw6G024020 Rw6G024030 Rw6G035790 Rw7G038130

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1434, 1469
AccB1I GGYRCC 2 cut(s) 389, 1777
AccB7I CCANNNNNTGG 1 cut(s) 282
AccBSI CCGCTC 1 cut(s) 85
AccI GTMKAC 1 cut(s) 41
AccII CGCG 3 cut(s) 1714, 1724, 2053
AciI CCGC 8 cut(s) 83, 1035, 1762, 1874, 1877, 1970, 2041, 2097
AclI AACGTT 1 cut(s) 1463
AclWI GGATC 6 cut(s) 475, 1439, 1939, 1985, 1990, 2029
AcoI YGGCCR 1 cut(s) 1410
AcsI RAATTY 3 cut(s) 484, 754, 903
AcyI GRCGYC 1 cut(s) 1778
AfaI GTAC 5 cut(s) 19, 33, 1022, 1054, 2084
AfeI AGCGCT 1 cut(s) 2123
AfiI CCNNNNNNNGG 9 cut(s) 282, 413, 661, 1064, 1155, 1481, 1950, 2018, 2108
AflIII ACRYGT 1 cut(s) 738
AgsI TTSAA 4 cut(s) 490, 854, 937, 1316
AhlI ACTAGT 1 cut(s) 23
AjiI CACGTC 1 cut(s) 739
AjnI CCWGG 4 cut(s) 199, 721, 1516, 1943
AloI GAACNNNNNNTCC 2 cut(s) 409, 441
Alw21I GWGCWC 1 cut(s) 220
Alw26I GTCTC 4 cut(s) 1413, 1543, 1601, 1859
AlwI GGATC 6 cut(s) 475, 1439, 1939, 1985, 1990, 2029
AlwNI CAGNNNCTG 2 cut(s) 52, 821
Ama87I CYCGRG 2 cut(s) 102, 1807
Aor51HI AGCGCT 1 cut(s) 2123
AoxI GGCC 2 cut(s) 1410, 1907
ApeKI GCWGC 5 cut(s) 62, 545, 1094, 1940, 2119
ApoI RAATTY 3 cut(s) 484, 754, 903
AseI ATTAAT 1 cut(s) 1695
AspLEI GCGC 5 cut(s) 1716, 1726, 1780, 2053, 2124
AspS9I GGNCC 7 cut(s) 203, 514, 1067, 1622, 1833, 2008, 2099
AsuHPI GGTGA 1 cut(s) 265
AvaI CYCGRG 2 cut(s) 102, 1807
AvaII GGWCC 7 cut(s) 203, 514, 1067, 1622, 1833, 2008, 2099
BaeGI GKGCMC 1 cut(s) 1601
BanI GGYRCC 2 cut(s) 389, 1777
BanII GRGCYC 2 cut(s) 220, 1771
Bbv12I GWGCWC 1 cut(s) 220
BbvI GCAGC 4 cut(s) 49, 557, 1081, 1927
BccI CCATC 6 cut(s) 301, 701, 1300, 1324, 1479, 1834
BceAI ACGGC 4 cut(s) 691, 1765, 1791, 2110
BciT130I CCWGG 4 cut(s) 201, 723, 1518, 1945
BciVI GTATCC 2 cut(s) 223, 987
BcoDI GTCTC 4 cut(s) 1413, 1543, 1601, 1859
BcuI ACTAGT 1 cut(s) 23
BfaI CTAG 6 cut(s) 24, 153, 372, 1241, 1283, 1829
BfmI CTRYAG 2 cut(s) 804, 1153
BfoI RGCGCY 2 cut(s) 1781, 2125
BfuI GTATCC 2 cut(s) 223, 987
BglI GCCNNNNNGGC 2 cut(s) 1775, 1915
BisI GCNGC 7 cut(s) 63, 546, 1095, 1763, 1875, 1941, 2120
BlpI GCTNAGC 1 cut(s) 961
BlsI GCNGC 7 cut(s) 64, 547, 1096, 1764, 1876, 1942, 2121
BmcAI AGTACT 2 cut(s) 19, 33
Bme1390I CCNGG 4 cut(s) 201, 723, 1518, 1945
Bme18I GGWCC 7 cut(s) 203, 514, 1067, 1622, 1833, 2008, 2099
BmeT110I CYCGRG 2 cut(s) 102, 1807
BmgBI CACGTC 1 cut(s) 739
BmgT120I GGNCC 7 cut(s) 203, 514, 1067, 1622, 1833, 2008, 2099
BmrFI CCNGG 4 cut(s) 201, 723, 1518, 1945
BmrI ACTGGG 1 cut(s) 903
BmsI GCATC 2 cut(s) 826, 919
BmuI ACTGGG 1 cut(s) 903
BoxI GACNNNNGTC 3 cut(s) 507, 1271, 2097
BpmI CTGGAG 2 cut(s) 1041, 1611
Bpu10I CCTNAGC 1 cut(s) 435
Bpu1102I GCTNAGC 1 cut(s) 961
Bsa29I ATCGAT 1 cut(s) 618
BsaAI YACGTR 1 cut(s) 1456
BsaHI GRCGYC 1 cut(s) 1778
BsaJI CCNNGG 4 cut(s) 721, 1261, 1889, 2061
BsaXI ACNNNNNCTCC 4 cut(s) 288, 318, 1326, 1356
Bsc4I CCNNNNNNNGG 9 cut(s) 282, 413, 661, 1064, 1155, 1481, 1950, 2018, 2108
Bse118I RCCGGY 1 cut(s) 1171
Bse1I ACTGG 4 cut(s) 29, 287, 371, 909
Bse3DI GCAATG 1 cut(s) 639
BseBI CCWGG 4 cut(s) 201, 723, 1518, 1945
BseCI ATCGAT 1 cut(s) 618
BseDI CCNNGG 4 cut(s) 721, 1261, 1889, 2061
BseGI GGATG 3 cut(s) 75, 1348, 1490
BseLI CCNNNNNNNGG 9 cut(s) 282, 413, 661, 1064, 1155, 1481, 1950, 2018, 2108
BseMI GCAATG 1 cut(s) 639
BseMII CTCAG 3 cut(s) 426, 1596, 1719
BseNI ACTGG 4 cut(s) 29, 287, 371, 909
BseRI GAGGAG 2 cut(s) 464, 1877
BseSI GKGCMC 1 cut(s) 1601
BseXI GCAGC 4 cut(s) 49, 557, 1081, 1927
Bsh1236I CGCG 3 cut(s) 1714, 1724, 2053
BshFI GGCC 2 cut(s) 1412, 1909
BshNI GGYRCC 2 cut(s) 389, 1777
BshVI ATCGAT 1 cut(s) 618
BsiHKAI GWGCWC 1 cut(s) 220
BsiHKCI CYCGRG 2 cut(s) 102, 1807
BsiSI CCGG 2 cut(s) 1172, 2102
BslFI GGGAC 5 cut(s) 275, 974, 1053, 1279, 1994
BslI CCNNNNNNNGG 9 cut(s) 282, 413, 661, 1064, 1155, 1481, 1950, 2018, 2108
BsmAI GTCTC 4 cut(s) 1413, 1543, 1601, 1859
BsmBI CGTCTC 1 cut(s) 1859
BsmFI GGGAC 5 cut(s) 275, 974, 1053, 1279, 1994
BsmI GAATGC 1 cut(s) 932
BsnI GGCC 2 cut(s) 1412, 1909
BsoBI CYCGRG 2 cut(s) 102, 1807
Bsp1286I GDGCHC 3 cut(s) 220, 1601, 1771
Bsp1407I TGTACA 1 cut(s) 1020
Bsp143I GATC 9 cut(s) 453, 467, 1243, 1444, 1931, 1977, 1982, 1996, 2021
Bsp1720I GCTNAGC 1 cut(s) 961
Bsp19I CCATGG 2 cut(s) 1889, 2061
BspACI CCGC 8 cut(s) 83, 1035, 1762, 1874, 1877, 1970, 2041, 2097
BspANI GGCC 2 cut(s) 1412, 1909
BspCNI CTCAG 3 cut(s) 427, 1597, 1720
BspDI ATCGAT 1 cut(s) 618
BspFNI CGCG 3 cut(s) 1714, 1724, 2053
BspPI GGATC 6 cut(s) 475, 1439, 1939, 1985, 1990, 2029
BspQI GCTCTTC 1 cut(s) 92
BspT107I GGYRCC 2 cut(s) 389, 1777
BsrBI CCGCTC 1 cut(s) 85
BsrDI GCAATG 1 cut(s) 639
BsrFI RCCGGY 1 cut(s) 1171
BsrGI TGTACA 1 cut(s) 1020
BsrI ACTGG 4 cut(s) 29, 287, 371, 909
BssAI RCCGGY 1 cut(s) 1171
BssECI CCNNGG 4 cut(s) 721, 1261, 1889, 2061
BssMI GATC 9 cut(s) 453, 467, 1243, 1444, 1931, 1977, 1982, 1996, 2021
BssNI GRCGYC 1 cut(s) 1778
BssT1I CCWWGG 3 cut(s) 1261, 1889, 2061
Bst2UI CCWGG 4 cut(s) 201, 723, 1518, 1945
Bst4CI ACNGT 7 cut(s) 48, 389, 661, 808, 817, 1258, 1300
Bst6I CTCTTC 4 cut(s) 8, 92, 441, 781
BstACI GRCGYC 1 cut(s) 1778
BstAUI TGTACA 1 cut(s) 1020
BstBAI YACGTR 1 cut(s) 1456
BstDEI CTNAG 5 cut(s) 435, 961, 1605, 1676, 1728
BstDSI CCRYGG 2 cut(s) 1889, 2061
BstENI CCTNNNNNAGG 2 cut(s) 1153, 1948
BstF5I GGATG 3 cut(s) 75, 1348, 1490
BstFNI CGCG 3 cut(s) 1714, 1724, 2053
BstH2I RGCGCY 2 cut(s) 1781, 2125
BstHHI GCGC 5 cut(s) 1716, 1726, 1780, 2053, 2124
BstKTI GATC 9 cut(s) 456, 470, 1246, 1447, 1934, 1980, 1985, 1999, 2024
BstMAI GTCTC 4 cut(s) 1413, 1543, 1601, 1859
BstMBI GATC 9 cut(s) 453, 467, 1243, 1444, 1931, 1977, 1982, 1996, 2021
BstMWI GCNNNNNNNGC 5 cut(s) 215, 675, 836, 1775, 1915
BstNI CCWGG 4 cut(s) 201, 723, 1518, 1945
BstNSI RCATGY 1 cut(s) 356
BstPAI GACNNNNGTC 3 cut(s) 507, 1271, 2097
BstSCI CCNGG 4 cut(s) 199, 721, 1516, 1943
BstSFI CTRYAG 2 cut(s) 804, 1153
BstSLI GKGCMC 1 cut(s) 1601
BstSNI TACGTA 1 cut(s) 1456
BstUI CGCG 3 cut(s) 1714, 1724, 2053
BstV1I GCAGC 4 cut(s) 49, 557, 1081, 1927
BstX2I RGATCY 2 cut(s) 467, 2021
BstXI CCANNNNNNTGG 1 cut(s) 1524
BstYI RGATCY 2 cut(s) 467, 2021
Bsu15I ATCGAT 1 cut(s) 618
BsuI GTATCC 2 cut(s) 223, 987
BsuRI GGCC 2 cut(s) 1412, 1909
BsuTUI ATCGAT 1 cut(s) 618
BtgI CCRYGG 2 cut(s) 1889, 2061
BtgZI GCGATG 1 cut(s) 1247
BtrI CACGTC 1 cut(s) 739
BtsCI GGATG 3 cut(s) 75, 1348, 1490
BtsIMutI CAGTG 3 cut(s) 280, 657, 1221
CaiI CAGNNNCTG 2 cut(s) 52, 821
CfoI GCGC 5 cut(s) 1716, 1726, 1780, 2053, 2124
Cfr10I RCCGGY 1 cut(s) 1171
Cfr13I GGNCC 7 cut(s) 203, 514, 1067, 1622, 1833, 2008, 2099
ClaI ATCGAT 1 cut(s) 618
CpoI CGGWCCG 1 cut(s) 2099
CseI GACGC 1 cut(s) 1090
Csp6I GTAC 5 cut(s) 18, 32, 1021, 1053, 2083
CspI CGGWCCG 1 cut(s) 2099
CviQI GTAC 5 cut(s) 18, 32, 1021, 1053, 2083
DdeI CTNAG 5 cut(s) 435, 961, 1605, 1676, 1728
DinI GGCGCC 1 cut(s) 1779
DpnI GATC 9 cut(s) 455, 469, 1245, 1446, 1933, 1979, 1984, 1998, 2023
DpnII GATC 9 cut(s) 453, 467, 1243, 1444, 1931, 1977, 1982, 1996, 2021
EaeI YGGCCR 1 cut(s) 1410
Eam1104I CTCTTC 4 cut(s) 8, 92, 441, 781
EarI CTCTTC 4 cut(s) 8, 92, 441, 781
EciI GGCGGA 2 cut(s) 1024, 2056
Ecl136II GAGCTC 1 cut(s) 218
Eco105I TACGTA 1 cut(s) 1456
Eco130I CCWWGG 3 cut(s) 1261, 1889, 2061
Eco24I GRGCYC 2 cut(s) 220, 1771
Eco47I GGWCC 7 cut(s) 203, 514, 1067, 1622, 1833, 2008, 2099
Eco47III AGCGCT 1 cut(s) 2123
Eco53kI GAGCTC 1 cut(s) 218
Eco88I CYCGRG 2 cut(s) 102, 1807
EcoICRI GAGCTC 1 cut(s) 218
EcoNI CCTNNNNNAGG 2 cut(s) 1153, 1948
EcoO109I RGGNCCY 3 cut(s) 1067, 1833, 2008
EcoRII CCWGG 4 cut(s) 199, 721, 1516, 1943
EcoT14I CCWWGG 3 cut(s) 1261, 1889, 2061
EcoT22I ATGCAT 1 cut(s) 934
EcoT38I GRGCYC 2 cut(s) 220, 1771
EgeI GGCGCC 1 cut(s) 1779
EheI GGCGCC 1 cut(s) 1779
ErhI CCWWGG 3 cut(s) 1261, 1889, 2061
Esp3I CGTCTC 1 cut(s) 1859
FaqI GGGAC 5 cut(s) 275, 974, 1053, 1279, 1994
FblI GTMKAC 1 cut(s) 41
Fnu4HI GCNGC 7 cut(s) 63, 546, 1095, 1763, 1875, 1941, 2120
FokI GGATG 3 cut(s) 62, 1335, 1497
FriOI GRGCYC 2 cut(s) 220, 1771
Fsp4HI GCNGC 7 cut(s) 63, 546, 1095, 1763, 1875, 1941, 2120
FspBI CTAG 6 cut(s) 24, 153, 372, 1241, 1283, 1829
GlaI GCGC 5 cut(s) 1715, 1725, 1779, 2052, 2123
GluI GCNGC 7 cut(s) 63, 546, 1095, 1763, 1875, 1941, 2120
GsuI CTGGAG 2 cut(s) 1041, 1611
HaeII RGCGCY 2 cut(s) 1781, 2125
HaeIII GGCC 2 cut(s) 1412, 1909
HapII CCGG 2 cut(s) 1172, 2102
HgaI GACGC 1 cut(s) 1090
HhaI GCGC 5 cut(s) 1716, 1726, 1780, 2053, 2124
Hin1I GRCGYC 1 cut(s) 1778
Hin6I GCGC 5 cut(s) 1714, 1724, 1778, 2051, 2122
HinP1I GCGC 5 cut(s) 1714, 1724, 1778, 2051, 2122
HincII GTYRAC 5 cut(s) 42, 502, 811, 1254, 1491
HindII GTYRAC 5 cut(s) 42, 502, 811, 1254, 1491
HindIII AAGCTT 2 cut(s) 958, 1956
HinfI GANTC 8 cut(s) 164, 824, 1133, 1345, 1421, 1571, 1661, 1805
HpaI GTTAAC 1 cut(s) 1491
HpaII CCGG 2 cut(s) 1172, 2102
HphI GGTGA 1 cut(s) 265
Hpy188III TCNNGA 6 cut(s) 434, 512, 1058, 1193, 1975, 2000
Hpy99I CGWCG 5 cut(s) 567, 1786, 1789, 2073, 2090
HpyAV CCTTC 5 cut(s) 329, 1650, 1907, 2038, 2101
HpyCH4III ACNGT 7 cut(s) 48, 389, 661, 808, 817, 1258, 1300
HpyCH4IV ACGT 5 cut(s) 557, 738, 1455, 1463, 2078
HpyCH4V TGCA 9 cut(s) 521, 845, 932, 1097, 1144, 1357, 1363, 1720, 2119
HpyF10VI GCNNNNNNNGC 5 cut(s) 215, 675, 836, 1775, 1915
HpyF3I CTNAG 5 cut(s) 435, 961, 1605, 1676, 1728
HpySE526I ACGT 5 cut(s) 557, 738, 1455, 1463, 2078
Hsp92I GRCGYC 1 cut(s) 1778
HspAI GCGC 5 cut(s) 1714, 1724, 1778, 2051, 2122
KasI GGCGCC 1 cut(s) 1777
KflI GGGWCCC 1 cut(s) 2008
KspAI GTTAAC 1 cut(s) 1491
Kzo9I GATC 9 cut(s) 453, 467, 1243, 1444, 1931, 1977, 1982, 1996, 2021
LguI GCTCTTC 1 cut(s) 92
LmnI GCTCC 5 cut(s) 683, 768, 889, 1755, 2048
Lsp1109I GCAGC 4 cut(s) 49, 557, 1081, 1927
LweI GCATC 2 cut(s) 826, 919
MaeI CTAG 6 cut(s) 24, 153, 372, 1241, 1283, 1829
MaeII ACGT 5 cut(s) 557, 738, 1455, 1463, 2078
MaeIII GTNAC 5 cut(s) 362, 863, 1456, 1538, 1679
MalI GATC 9 cut(s) 455, 469, 1245, 1446, 1933, 1979, 1984, 1998, 2023
MbiI CCGCTC 1 cut(s) 85
MboI GATC 9 cut(s) 453, 467, 1243, 1444, 1931, 1977, 1982, 1996, 2021
MflI RGATCY 2 cut(s) 467, 2021
MhlI GDGCHC 3 cut(s) 220, 1601, 1771
MluCI AATT 6 cut(s) 234, 484, 754, 903, 1435, 1688
Mly113I GGCGCC 1 cut(s) 1778
MlyI GAGTC 4 cut(s) 818, 1415, 1580, 1799
MmeI TCCRAC 3 cut(s) 763, 1767, 1827
Mph1103I ATGCAT 1 cut(s) 934
MseI TTAA 5 cut(s) 135, 243, 1215, 1490, 1695
MslI CAYNNNNRTG 1 cut(s) 1362
MspA1I CMGCKG 2 cut(s) 548, 1762
MspI CCGG 2 cut(s) 1172, 2102
MspR9I CCNGG 4 cut(s) 201, 723, 1518, 1945
Mva1269I GAATGC 1 cut(s) 932
MvaI CCWGG 4 cut(s) 201, 723, 1518, 1945
MvnI CGCG 3 cut(s) 1714, 1724, 2053
MwoI GCNNNNNNNGC 5 cut(s) 215, 675, 836, 1775, 1915
NarI GGCGCC 1 cut(s) 1778
NcoI CCATGG 2 cut(s) 1889, 2061
NdeII GATC 9 cut(s) 453, 467, 1243, 1444, 1931, 1977, 1982, 1996, 2021
NmuCI GTSAC 3 cut(s) 362, 1538, 1679
NsiI ATGCAT 1 cut(s) 934
NspI RCATGY 1 cut(s) 356
PaeI GCATGC 1 cut(s) 356
PaeR7I CTCGAG 1 cut(s) 102
PciSI GCTCTTC 1 cut(s) 92
PcsI WCGNNNNNNNCGW 2 cut(s) 615, 1781
PctI GAATGC 1 cut(s) 932
PfeI GAWTC 4 cut(s) 164, 1133, 1345, 1661
PflMI CCANNNNNTGG 1 cut(s) 282
PkrI GCNGC 7 cut(s) 64, 547, 1096, 1764, 1876, 1942, 2121
PleI GAGTC 4 cut(s) 818, 1415, 1579, 1799
PluTI GGCGCC 1 cut(s) 1781
PpsI GAGTC 4 cut(s) 818, 1415, 1579, 1799
Ppu21I YACGTR 1 cut(s) 1456
PpuMI RGGWCCY 3 cut(s) 1067, 1833, 2008
PshAI GACNNNNGTC 3 cut(s) 507, 1271, 2097
PshBI ATTAAT 1 cut(s) 1695
PsiI TTATAA 2 cut(s) 1434, 1469
Psp124BI GAGCTC 1 cut(s) 220
Psp1406I AACGTT 1 cut(s) 1463
Psp5II RGGWCCY 3 cut(s) 1067, 1833, 2008
Psp6I CCWGG 4 cut(s) 199, 721, 1516, 1943
PspGI CCWGG 4 cut(s) 199, 721, 1516, 1943
PspPI GGNCC 7 cut(s) 203, 514, 1067, 1622, 1833, 2008, 2099
PspPPI RGGWCCY 3 cut(s) 1067, 1833, 2008
PspXI VCTCGAGB 1 cut(s) 102
PstNI CAGNNNCTG 2 cut(s) 52, 821
PsuI RGATCY 2 cut(s) 467, 2021
PvuII CAGCTG 1 cut(s) 548
RsaI GTAC 5 cut(s) 19, 33, 1022, 1054, 2084
RsaNI GTAC 5 cut(s) 18, 32, 1021, 1053, 2083
RseI CAYNNNNRTG 1 cut(s) 1362
Rsr2I CGGWCCG 1 cut(s) 2099
RsrII CGGWCCG 1 cut(s) 2099
SacI GAGCTC 1 cut(s) 220
SalI GTCGAC 1 cut(s) 40
SapI GCTCTTC 1 cut(s) 92
SaqAI TTAA 5 cut(s) 135, 243, 1215, 1490, 1695
SatI GCNGC 7 cut(s) 63, 546, 1095, 1763, 1875, 1941, 2120
Sau3AI GATC 9 cut(s) 453, 467, 1243, 1444, 1931, 1977, 1982, 1996, 2021
Sau96I GGNCC 7 cut(s) 203, 514, 1067, 1622, 1833, 2008, 2099
ScaI AGTACT 2 cut(s) 19, 33
SchI GAGTC 4 cut(s) 818, 1415, 1580, 1799
ScrFI CCNGG 4 cut(s) 201, 723, 1518, 1945
SduI GDGCHC 3 cut(s) 220, 1601, 1771
SfaNI GCATC 2 cut(s) 826, 919
SfcI CTRYAG 2 cut(s) 804, 1153
SfoI GGCGCC 1 cut(s) 1779
Sfr274I CTCGAG 1 cut(s) 102
SinI GGWCC 7 cut(s) 203, 514, 1067, 1622, 1833, 2008, 2099
SlaI CTCGAG 1 cut(s) 102
SmiMI CAYNNNNRTG 1 cut(s) 1362
SmlI CTYRAG 1 cut(s) 102
SmoI CTYRAG 1 cut(s) 102
SnaBI TACGTA 1 cut(s) 1456
SpeI ACTAGT 1 cut(s) 23
SphI GCATGC 1 cut(s) 356
Sse9I AATT 6 cut(s) 234, 484, 754, 903, 1435, 1688
SsiI CCGC 8 cut(s) 83, 1035, 1762, 1874, 1877, 1970, 2041, 2097
SspDI GGCGCC 1 cut(s) 1777
SspI AATATT 1 cut(s) 952
SspMI CTAG 6 cut(s) 24, 153, 372, 1241, 1283, 1829
SstI GAGCTC 1 cut(s) 220
StyD4I CCNGG 4 cut(s) 199, 721, 1516, 1943
StyI CCWWGG 3 cut(s) 1261, 1889, 2061
TaaI ACNGT 7 cut(s) 48, 389, 661, 808, 817, 1258, 1300
TaiI ACGT 5 cut(s) 560, 741, 1458, 1466, 2081
TaqI TCGA 6 cut(s) 41, 103, 618, 1246, 1838, 2068
TaqII GACCGA 1 cut(s) 1610
TasI AATT 6 cut(s) 234, 484, 754, 903, 1435, 1688
TatI WGTACW 4 cut(s) 17, 31, 1020, 1052
TauI GCSGC 2 cut(s) 1765, 1877
TfiI GAWTC 4 cut(s) 164, 1133, 1345, 1661
Tru1I TTAA 5 cut(s) 135, 243, 1215, 1490, 1695
Tru9I TTAA 5 cut(s) 135, 243, 1215, 1490, 1695
TscAI CASTG 3 cut(s) 287, 664, 1228
TseFI GTSAC 3 cut(s) 362, 1538, 1679
TseI GCWGC 5 cut(s) 62, 545, 1094, 1940, 2119
Tsp45I GTSAC 3 cut(s) 362, 1538, 1679
TspDTI ATGAA 7 cut(s) 17, 26, 79, 104, 459, 1194, 1308
TspGWI ACGGA 3 cut(s) 960, 1330, 1497
TspRI CASTG 3 cut(s) 287, 664, 1228
Van91I CCANNNNNTGG 1 cut(s) 282
VpaK11BI GGWCC 7 cut(s) 203, 514, 1067, 1622, 1833, 2008, 2099
VspI ATTAAT 1 cut(s) 1695
XagI CCTNNNNNAGG 2 cut(s) 1153, 1948
XapI RAATTY 3 cut(s) 484, 754, 903
XceI RCATGY 1 cut(s) 356
XhoI CTCGAG 1 cut(s) 102
XmiI GTMKAC 1 cut(s) 41
XspI CTAG 6 cut(s) 24, 153, 372, 1241, 1283, 1829
ZrmI AGTACT 2 cut(s) 19, 33
Zsp2I ATGCAT 1 cut(s) 934
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.