FvH4_3g32806

IAA-amino acid hydrolase ILR1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000136
Physical Location & Seq
Reverse (-)
28155956 .. 28160164
4209 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g32806.t1

Sequence Viewer

Length: 993 bp
ATGGTGGTTGTGGTCATCGGGAGGAGAGGTTTTCGGTTTTCATCTGACCGGTGGCGGTACAAGACTGTATCGAATTGGCGGCGGCGGGTTTGCACTGGTGGCGGGTTTGCGCTGGCAGTGGCTTCTTCAGTCAAGTCGTGTCGAATCTCTAGAGGTCGACGGCGGCGGCTTCTTCGGTCAACCCTAATCGCCCATCTTTTCCCTACTCTTCCCTGCTCGAGCTCGCAGAAGGTACTCTCTTCCCTGCTCGAGCTCGCGCATCTCTTCCATGCTCTTCCCTGCTCAAGCTCGCAGAAGGTACTCTTTTCCCTGCTCGAGCTCGCAGAAGGGGAGTGTGGAGTGGGGGAGCACAAGAGTAAAGTTGCAGGGAAGATGCATGGATGTGGACATGATGGTCATGTTGCCATGCTTCTTGGAGCTGCTAGTTTACTTCAAGAGTATCGCCACAAATTACAGGGGACTGTTGTTCTTGTTTTCCAACCAGCTGAGGAAGGAGGTGCAGGAGCAAAGAAAATGATAGAAAACGGGGCACTAGAGCATGTCAGTGCCATCTTTGGGTTGCATCTTGCTTCTGACCATCCCATTGGTTCAGTAGCCTCCAAACCTGGCCCTAGAATGGCTGCGAGTGGATTTTTTGAAGCCTTGATAAGTGGGAAGGGGGGTCGTGCTGCAATTCCACAGCACACAATAGATCCTATCTTGGCTGCTTCCAATGTTATTGTTAGTCTGCAGCATCTTGTTTCACTTGAAGCTGACCCCTTAGATTCACAGGTTTGCTCGCTTTCTCTCTATTCTCATAATAAGGTCATTGTCAAGTCTATGTTCCCCTCCTATTGCAGCTTGATTATTCAAATACATGTATCTGTACCAGCTGATGCAACAAGTGGCCATGAGGCTAGGAGGGAGTCCATAGGAGAGGGAGATCCTGAGGCCACTACCCATTTTGATTCAACTTCTGATGATGTCACTGGTCACCATGATCCTCCTGCATGA

Protein Analysis

331

Amino Acids

35.3

Weight (kDa)

8.92

Isoelectric Point (pI)

49.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M20 PF01546 114 - 268 5.8e-24 Peptidase family M20/M25/M40
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000136)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g28181 FvH4_1g28182 FvH4_1g28940 FvH4_1g29801 FvH4_1g29802 FvH4_3g03321 FvH4_3g12341 FvH4_3g22002 FvH4_3g22003 FvH4_3g22852 FvH4_3g22860 FvH4_3g32806 FvH4_4g02410 FvH4_4g08110 FvH4_4g09791 FvH4_4g09792 FvH4_4g09830 FvH4_4g09831 FvH4_4g09832 FvH4_4g23330 FvH4_5g25220 FvH4_5g30160 FvH4_5g32340 FvH4_5g37421 FvH4_6g21851 FvH4_6g21852 FvH4_6g22855 FvH4_6g22870 FvH4_6g30440 FvH4_6g30962 FvH4_6g37930
malus_domestica MD16G1158000.v1.1
prunus_persica Prupe.1G266000_v2.0.a1 Prupe.1G266100_v2.0.a1 Prupe.1G266200_v2.0.a1 Prupe.2G006600_v2.0.a1 Prupe.2G110300_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0322121 RchiOBHm_Chr1g0322131 RchiOBHm_Chr1g0341871 RchiOBHm_Chr1g0346721 RchiOBHm_Chr2g0118081 RchiOBHm_Chr2g0138651 RchiOBHm_Chr3g0468651 RchiOBHm_Chr3g0475981 RchiOBHm_Chr4g0416921 RchiOBHm_Chr5g0068021 RchiOBHm_Chr6g0273901 RchiOBHm_Chr6g0278661 RchiOBHm_Chr6g0281481 RchiOBHm_Chr6g0306081 RchiOBHm_Chr6g0306091 RchiOBHm_Chr7g0220731 RchiOBHm_Chr7g0220741 RchiOBHm_Chr7g0222641 RchiOBHm_Chr7g0225401 RchiOBHm_Chr7g0230481
rosa_laevigata RLG00000001569 RLG00000006184 RLG00000007906 RLG00000009863 RLG00000011359 RLG00000016125 RLG00000018543 RLG00000020693 RLG00000029658 RLG00000029716
rosa_multiflora Rmu_co8244591.1_g000001 Rmu_co8396391.1_g000001 Rmu_sc0000120.1_g000013 Rmu_sc0000239.1_g000047 Rmu_sc0000252.1_g000019 Rmu_sc0000322.1_g000037 Rmu_sc0000376.1_g000010 Rmu_sc0000435.1_g000033 Rmu_sc0001872.1_g000001 Rmu_sc0001992.1_g000011 Rmu_sc0001992.1_g000012 Rmu_sc0002231.1_g000010 Rmu_sc0002372.1_g000033 Rmu_sc0002705.1_g000012 Rmu_sc0003435.1_g000015 Rmu_sc0004084.1_g000042 Rmu_sc0004084.1_g000043 Rmu_sc0004755.1_g000011 Rmu_sc0005391.1_g000010 Rmu_sc0005970.1_g000001 Rmu_sc0006163.1_g000003 Rmu_sc0006422.1_g000004 Rmu_sc0006422.1_g000005 Rmu_sc0006952.1_g000002 Rmu_sc0010937.1_g000007 Rmu_sc0012130.1_g000008 Rmu_sc0024120.1_g000001 Rmu_sc0035618.1_g000001
rosa_roxburghii Rroxscaffold_1G00039230 Rroxscaffold_2G00085900 Rroxscaffold_2G00085910 Rroxscaffold_2G00125240 Rroxscaffold_2G00126850 Rroxscaffold_2G00150980 Rroxscaffold_3G00230580 Rroxscaffold_3G00245050 Rroxscaffold_4G00320680 Rroxscaffold_4G00328800 Rroxscaffold_5G00349640 Rroxscaffold_6G00406600 Rroxscaffold_7G00165570 Rroxscaffold_7G00191490 Rroxscaffold_7G00192700 Rroxscaffold_7G00192710 Rroxscaffold_7G00201190 Rroxscaffold_7G00201620
rosa_rugosa Rorug01G0033100 Rorug01G0089100 Rorug01G0102000 Rorug01G0353100 Rorug01G0353200 Rorug01G0353300 Rorug02G0276900 Rorug02G0362200 Rorug02G0445100 Rorug02G0627600 Rorug02G0627600 Rorug02G0627600 Rorug03G0267700 Rorug03G0267800 Rorug03G0327400 Rorug04G0004500 Rorug04G0006700 Rorug04G0061500 Rorug04G0064700 Rorug04G0073300 Rorug04G0094300 Rorug04G0181200 Rorug04G0181200 Rorug04G0330100 Rorug05G0035600 Rorug05G0038600 Rorug05G0201600 Rorug05G0367700 Rorug05G0407600 Rorug05G0407700 Rorug05G0543400 Rorug06G0012200 Rorug06G0363700.1 Rorug07G0085100
rosa_samantha Rh1AG049700 Rh1AG182700 Rh1AG182800 Rh1BG015500 Rh1BG398700 Rh1BG409100 Rh1CG074100 Rh1CG169200 Rh1CG169300 Rh1CG169400 Rh1DG130800 Rh1DG130900 Rh1DG440400 Rh2DG440900 Rh3AG144700 Rh3BG167100 Rh3DG366800 Rh4AG214400 Rh4BG189700 Rh4BG189800 Rh4CG268600 Rh4DG004400 Rh4DG147900 Rh4DG248300 Rh5AG053900 Rh5AG054000 Rh5AG317200 Rh5AG326400 Rh5AG332600 Rh5AG419700 Rh5CG284900 Rh5CG288600 Rh6AG252700 Rh6AG252800 Rh6AG256900 Rh6AG257000 Rh6AG257500 Rh6BG175300 Rh6DG226200 Rh6DG264500 Rh7AG374000 Rh7AG398100 Rh7BG274900 Rh7BG300500 Rh7BG308200 Rh7BG451200
rosa_wichuraiana Rw1G015860 Rw1G022160 Rw3G016160 Rw3G021170 Rw4G006020 Rw4G008740 Rw5G027400 Rw5G031840 Rw5G036940 Rw6G008320 Rw6G030330 Rw7G033450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 393
AccI GTMKAC 1 cut(s) 157
AccII CGCG 1 cut(s) 257
AciI CCGC 7 cut(s) 55, 79, 82, 85, 102, 163, 166
AclWI GGATC 3 cut(s) 686, 917, 974
AcoI YGGCCR 1 cut(s) 886
AcuI CTGAAG 1 cut(s) 111
AfaI GTAC 4 cut(s) 59, 234, 300, 867
AfiI CCNNNNNNNGG 2 cut(s) 555, 616
AflIII ACRYGT 1 cut(s) 856
AgeI ACCGGT 1 cut(s) 48
AgsI TTSAA 5 cut(s) 434, 638, 749, 851, 951
AjnI CCWGG 1 cut(s) 604
AluBI AGCT 9 cut(s) 222, 253, 288, 319, 419, 485, 752, 840, 872
AluI AGCT 9 cut(s) 222, 253, 288, 319, 419, 485, 752, 840, 872
Alw21I GWGCWC 4 cut(s) 224, 255, 321, 351
AlwI GGATC 3 cut(s) 686, 917, 974
Ama87I CYCGRG 3 cut(s) 217, 248, 314
AoxI GGCC 3 cut(s) 607, 886, 930
ApeKI GCWGC 6 cut(s) 419, 620, 668, 704, 730, 837
AsiGI ACCGGT 1 cut(s) 48
AspLEI GCGC 2 cut(s) 112, 259
AspS9I GGNCC 1 cut(s) 608
AsuHPI GGTGA 1 cut(s) 965
AvaI CYCGRG 3 cut(s) 217, 248, 314
AxyI CCTNAGG 1 cut(s) 927
BaeGI GKGCMC 1 cut(s) 532
BalI TGGCCA 1 cut(s) 888
BanII GRGCYC 3 cut(s) 224, 255, 321
Bbv12I GWGCWC 4 cut(s) 224, 255, 321, 351
BbvCI CCTCAGC 1 cut(s) 486
BbvI GCAGC 6 cut(s) 406, 607, 655, 691, 742, 849
BccI CCATC 4 cut(s) 201, 386, 557, 585
BceAI ACGGC 1 cut(s) 176
BciT130I CCWGG 1 cut(s) 606
BfaI CTAG 5 cut(s) 150, 423, 533, 612, 897
BfmI CTRYAG 1 cut(s) 728
Bme1390I CCNGG 1 cut(s) 606
BmeT110I CYCGRG 3 cut(s) 217, 248, 314
BmgT120I GGNCC 1 cut(s) 608
BmrFI CCNGG 1 cut(s) 606
BmsI GCATC 5 cut(s) 268, 363, 571, 742, 865
Bpu10I CCTNAGC 1 cut(s) 486
BpuEI CTTGAG 1 cut(s) 268
BsaWI WCCGGW 1 cut(s) 48
BsaXI ACNNNNNCTCC 2 cut(s) 13, 43
Bsc4I CCNNNNNNNGG 2 cut(s) 555, 616
Bse118I RCCGGY 1 cut(s) 48
Bse1I ACTGG 2 cut(s) 100, 973
Bse21I CCTNAGG 1 cut(s) 927
BseBI CCWGG 1 cut(s) 606
BseGI GGATG 2 cut(s) 386, 577
BseLI CCNNNNNNNGG 2 cut(s) 555, 616
BseMII CTCAG 2 cut(s) 477, 918
BseNI ACTGG 2 cut(s) 100, 973
BseRI GAGGAG 1 cut(s) 37
BseSI GKGCMC 1 cut(s) 532
BseXI GCAGC 6 cut(s) 406, 607, 655, 691, 742, 849
BsgI GTGCAG 1 cut(s) 519
Bsh1236I CGCG 1 cut(s) 257
BshFI GGCC 3 cut(s) 609, 888, 932
BshTI ACCGGT 1 cut(s) 48
BsiHKAI GWGCWC 4 cut(s) 224, 255, 321, 351
BsiHKCI CYCGRG 3 cut(s) 217, 248, 314
BsiSI CCGG 1 cut(s) 49
BslFI GGGAC 1 cut(s) 472
BslI CCNNNNNNNGG 2 cut(s) 555, 616
BsmFI GGGAC 1 cut(s) 472
BsnI GGCC 3 cut(s) 609, 888, 932
BsoBI CYCGRG 3 cut(s) 217, 248, 314
Bsp1286I GDGCHC 5 cut(s) 224, 255, 321, 351, 532
Bsp143I GATC 3 cut(s) 691, 922, 979
BspACI CCGC 7 cut(s) 55, 79, 82, 85, 102, 163, 166
BspANI GGCC 3 cut(s) 609, 888, 932
BspCNI CTCAG 2 cut(s) 478, 919
BspFNI CGCG 1 cut(s) 257
BspMAI CTGCAG 1 cut(s) 732
BspPI GGATC 3 cut(s) 686, 917, 974
BspQI GCTCTTC 1 cut(s) 279
BsrFI RCCGGY 1 cut(s) 48
BsrI ACTGG 2 cut(s) 100, 973
BssAI RCCGGY 1 cut(s) 48
BssMI GATC 3 cut(s) 691, 922, 979
Bst2UI CCWGG 1 cut(s) 606
Bst4CI ACNGT 2 cut(s) 67, 463
Bst6I CTCTTC 4 cut(s) 213, 244, 269, 279
BstC8I GCNNGC 6 cut(s) 114, 224, 255, 290, 321, 779
BstDEI CTNAG 3 cut(s) 486, 760, 927
BstEII GGTNACC 1 cut(s) 971
BstF5I GGATG 2 cut(s) 386, 577
BstFNI CGCG 1 cut(s) 257
BstHHI GCGC 2 cut(s) 112, 259
BstKTI GATC 3 cut(s) 694, 925, 982
BstMBI GATC 3 cut(s) 691, 922, 979
BstMWI GCNNNNNNNGC 1 cut(s) 99
BstNI CCWGG 1 cut(s) 606
BstNSI RCATGY 2 cut(s) 542, 860
BstPI GGTNACC 1 cut(s) 971
BstSCI CCNGG 1 cut(s) 604
BstSFI CTRYAG 1 cut(s) 728
BstSLI GKGCMC 1 cut(s) 532
BstUI CGCG 1 cut(s) 257
BstV1I GCAGC 6 cut(s) 406, 607, 655, 691, 742, 849
BstX2I RGATCY 2 cut(s) 691, 922
BstXI CCANNNNNNTGG 1 cut(s) 584
BstYI RGATCY 2 cut(s) 691, 922
Bsu36I CCTNAGG 1 cut(s) 927
BsuRI GGCC 3 cut(s) 609, 888, 932
BtsCI GGATG 2 cut(s) 386, 577
BtsI GCAGTG 1 cut(s) 123
BtsIMutI CAGTG 4 cut(s) 93, 123, 550, 966
Cac8I GCNNGC 6 cut(s) 114, 224, 255, 290, 321, 779
CfoI GCGC 2 cut(s) 112, 259
Cfr10I RCCGGY 1 cut(s) 48
Cfr13I GGNCC 1 cut(s) 608
Csp6I GTAC 4 cut(s) 58, 233, 299, 866
CspAI ACCGGT 1 cut(s) 48
CviQI GTAC 4 cut(s) 58, 233, 299, 866
DdeI CTNAG 3 cut(s) 486, 760, 927
DpnI GATC 3 cut(s) 693, 924, 981
DpnII GATC 3 cut(s) 691, 922, 979
DrdI GACNNNNNNGTC 1 cut(s) 393
DseDI GACNNNNNNGTC 1 cut(s) 393
EaeI YGGCCR 1 cut(s) 886
Eam1104I CTCTTC 4 cut(s) 213, 244, 269, 279
EarI CTCTTC 4 cut(s) 213, 244, 269, 279
Ecl136II GAGCTC 3 cut(s) 222, 253, 319
Eco24I GRGCYC 3 cut(s) 224, 255, 321
Eco53kI GAGCTC 3 cut(s) 222, 253, 319
Eco57I CTGAAG 1 cut(s) 111
Eco81I CCTNAGG 1 cut(s) 927
Eco88I CYCGRG 3 cut(s) 217, 248, 314
Eco91I GGTNACC 1 cut(s) 971
EcoICRI GAGCTC 3 cut(s) 222, 253, 319
EcoO65I GGTNACC 1 cut(s) 971
EcoRII CCWGG 1 cut(s) 604
EcoT22I ATGCAT 1 cut(s) 378
EcoT38I GRGCYC 3 cut(s) 224, 255, 321
FalI AAGNNNNNCTT 2 cut(s) 287, 319
FaqI GGGAC 1 cut(s) 472
FauI CCCGC 2 cut(s) 78, 95
FblI GTMKAC 1 cut(s) 157
FokI GGATG 2 cut(s) 393, 564
FriOI GRGCYC 3 cut(s) 224, 255, 321
FspBI CTAG 5 cut(s) 150, 423, 533, 612, 897
GlaI GCGC 2 cut(s) 111, 258
HaeIII GGCC 3 cut(s) 609, 888, 932
HapII CCGG 1 cut(s) 49
HhaI GCGC 2 cut(s) 112, 259
Hin6I GCGC 2 cut(s) 110, 257
HinP1I GCGC 2 cut(s) 110, 257
HincII GTYRAC 2 cut(s) 158, 180
HindII GTYRAC 2 cut(s) 158, 180
HinfI GANTC 4 cut(s) 144, 764, 905, 947
HpaII CCGG 1 cut(s) 49
HphI GGTGA 1 cut(s) 965
Hpy166II GTNNAC 4 cut(s) 158, 180, 386, 428
Hpy188I TCNGA 3 cut(s) 46, 574, 958
Hpy188III TCNNGA 4 cut(s) 19, 150, 434, 926
Hpy8I GTNNAC 4 cut(s) 158, 180, 386, 428
Hpy99I CGWCG 1 cut(s) 162
HpyAV CCTTC 5 cut(s) 223, 289, 320, 485, 649
HpyCH4III ACNGT 2 cut(s) 67, 463
HpyF10VI GCNNNNNNNGC 1 cut(s) 99
HpyF3I CTNAG 3 cut(s) 486, 760, 927
HspAI GCGC 2 cut(s) 110, 257
Kzo9I GATC 3 cut(s) 691, 922, 979
LguI GCTCTTC 1 cut(s) 279
LmnI GCTCC 3 cut(s) 346, 416, 503
Lsp1109I GCAGC 6 cut(s) 406, 607, 655, 691, 742, 849
LweI GCATC 5 cut(s) 268, 363, 571, 742, 865
MaeI CTAG 5 cut(s) 150, 423, 533, 612, 897
MaeIII GTNAC 2 cut(s) 964, 971
MalI GATC 3 cut(s) 693, 924, 981
MboI GATC 3 cut(s) 691, 922, 979
MboII GAAGA 7 cut(s) 117, 164, 200, 231, 256, 266, 382
MflI RGATCY 2 cut(s) 691, 922
MhlI GDGCHC 5 cut(s) 224, 255, 321, 351, 532
MlsI TGGCCA 1 cut(s) 888
MluCI AATT 3 cut(s) 73, 449, 672
MluNI TGGCCA 1 cut(s) 888
MlyI GAGTC 1 cut(s) 914
MmeI TCCRAC 1 cut(s) 502
Mox20I TGGCCA 1 cut(s) 888
Mph1103I ATGCAT 1 cut(s) 378
MscI TGGCCA 1 cut(s) 888
MslI CAYNNNNRTG 2 cut(s) 381, 543
Msp20I TGGCCA 1 cut(s) 888
MspA1I CMGCKG 2 cut(s) 485, 872
MspI CCGG 1 cut(s) 49
MspR9I CCNGG 1 cut(s) 606
MvaI CCWGG 1 cut(s) 606
MvnI CGCG 1 cut(s) 257
MwoI GCNNNNNNNGC 1 cut(s) 99
NdeII GATC 3 cut(s) 691, 922, 979
NmuCI GTSAC 2 cut(s) 964, 971
NsiI ATGCAT 1 cut(s) 378
NspI RCATGY 2 cut(s) 542, 860
PaeR7I CTCGAG 3 cut(s) 217, 248, 314
PciI ACATGT 1 cut(s) 856
PciSI GCTCTTC 1 cut(s) 279
PfeI GAWTC 3 cut(s) 144, 764, 947
PinAI ACCGGT 1 cut(s) 48
PleI GAGTC 1 cut(s) 913
PpsI GAGTC 1 cut(s) 913
PscI ACATGT 1 cut(s) 856
Psp124BI GAGCTC 3 cut(s) 224, 255, 321
Psp6I CCWGG 1 cut(s) 604
PspEI GGTNACC 1 cut(s) 971
PspGI CCWGG 1 cut(s) 604
PspPI GGNCC 1 cut(s) 608
PspXI VCTCGAGB 3 cut(s) 217, 248, 314
PstI CTGCAG 1 cut(s) 732
PsuI RGATCY 2 cut(s) 691, 922
PvuII CAGCTG 2 cut(s) 485, 872
RsaI GTAC 4 cut(s) 59, 234, 300, 867
RsaNI GTAC 4 cut(s) 58, 233, 299, 866
RseI CAYNNNNRTG 2 cut(s) 381, 543
SacI GAGCTC 3 cut(s) 224, 255, 321
SalI GTCGAC 1 cut(s) 156
SapI GCTCTTC 1 cut(s) 279
Sau3AI GATC 3 cut(s) 691, 922, 979
Sau96I GGNCC 1 cut(s) 608
SchI GAGTC 1 cut(s) 914
ScrFI CCNGG 1 cut(s) 606
SduI GDGCHC 5 cut(s) 224, 255, 321, 351, 532
SfaNI GCATC 5 cut(s) 268, 363, 571, 742, 865
SfcI CTRYAG 1 cut(s) 728
Sfr274I CTCGAG 3 cut(s) 217, 248, 314
SlaI CTCGAG 3 cut(s) 217, 248, 314
SmiMI CAYNNNNRTG 2 cut(s) 381, 543
SmlI CTYRAG 4 cut(s) 217, 248, 283, 314
SmoI CTYRAG 4 cut(s) 217, 248, 283, 314
Sse9I AATT 3 cut(s) 73, 449, 672
SsiI CCGC 7 cut(s) 55, 79, 82, 85, 102, 163, 166
SspMI CTAG 5 cut(s) 150, 423, 533, 612, 897
SstI GAGCTC 3 cut(s) 224, 255, 321
StyD4I CCNGG 1 cut(s) 604
TaaI ACNGT 2 cut(s) 67, 463
TaqI TCGA 6 cut(s) 71, 142, 157, 218, 249, 315
TaqII GACCGA 1 cut(s) 165
TasI AATT 3 cut(s) 73, 449, 672
TauI GCSGC 4 cut(s) 82, 85, 166, 169
TfiI GAWTC 3 cut(s) 144, 764, 947
TscAI CASTG 4 cut(s) 100, 123, 550, 973
TseFI GTSAC 2 cut(s) 964, 971
TseI GCWGC 6 cut(s) 419, 620, 668, 704, 730, 837
Tsp45I GTSAC 2 cut(s) 964, 971
TspDTI ATGAA 1 cut(s) 30
TspRI CASTG 4 cut(s) 100, 123, 550, 973
XbaI TCTAGA 1 cut(s) 149
XceI RCATGY 2 cut(s) 542, 860
XhoI CTCGAG 3 cut(s) 217, 248, 314
XmiI GTMKAC 1 cut(s) 157
XspI CTAG 5 cut(s) 150, 423, 533, 612, 897
Zsp2I ATGCAT 1 cut(s) 378
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.