FvH4_4g02410

IAA-amino acid hydrolase ILR1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000136
Physical Location & Seq
Forward (+)
2218220 .. 2223167
4948 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g02410.t1

Sequence Viewer

Length: 999 bp
ATGAAATTGGGGTTGGTCGTGAGAAACACAAATCATCGACTGTGGAAGGAGAGCTTGTCGACGGTGGTTGTGGTCATCGGGAGGAGAGGTTTTCGGTTTTCATCTGACCGGTGGCGGTACAAGACTGTATTGAATTGGCGGCGGCGGGTTTGCACTGGTGGCGGGTTTGCGCTGGCAGTGGCTTCTTCGGTCAAGTCGTGTCGAATCTCTAGAGGTCGACGGCGGCGGCTTCTTCGGTCAACCCTAATCGCCCATCTTTTCCCTGCTCTTCCCTGCTCGAGCTCGCAGAAGGTACTCTCTTCCCTGCTCGAGCTCGCGCATCTCTTCCATGCTCTTCCCTGCTCGAGCTCGCAGAAGGTACTCTTTTCCCTGCTCGAGCTCGCAGAAGGGGAGTGTGGAGTGGGGGAGCACAAGAGTAAAGTTGCAGGGAAGATGCATGGATGTGGACATGATGGTCATGTTGCCATGCTTCTTGGAGCTGCTAGTTTACTTCAAGAGTATCGCCACAAATTACAGGGGACTGTTGTTCTTGTTTTCCAACCAGCTGAGGAAGGAGGTGCAGGAGCAAAGAAAATGATAGAAGACGGGGCACTGGAGCATGTCAGTGCCATCTTTGGGTTGCATCTTGCTTCTGACCATCCCATTGGTTCAGTAGCCTCTAAACCTGGCCTTAGAATGGCTGCGAGTGGATTTTTTGAAGCCTTGATAAGTGGGAAGGGGGGTCGTGCTGCAATTCCACAGCACACAATAGATCCTATCTTGGCTGCTTCCAATGTTATTGTTAGTCTGCAGCATCTTGTTTCACGTAAAGCTGACCCCTTAGATTCACAGTCGCTATTTGCCGTCGCAAACATGGTTGTGACGGCTCACTGCCGTCGCCAAAAGCAACGGCCACACCCTCAACGGCGACGGGAAAAATGCCGTCGCCACGCCGTCGCCGTTGGTCTTTTGCGACGGCAGAATGACTTTTGGCGACGGCATTTTGCCGTGGCTAACTGA

Protein Analysis

333

Amino Acids

36.48

Weight (kDa)

10.98

Isoelectric Point (pI)

60.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M20 PF01546 134 - 260 1e-23 Peptidase family M20/M25/M40
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000136)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g28181 FvH4_1g28182 FvH4_1g28940 FvH4_1g29801 FvH4_1g29802 FvH4_3g03321 FvH4_3g12341 FvH4_3g22002 FvH4_3g22003 FvH4_3g22852 FvH4_3g22860 FvH4_3g32806 FvH4_4g02410 FvH4_4g08110 FvH4_4g09791 FvH4_4g09792 FvH4_4g09830 FvH4_4g09831 FvH4_4g09832 FvH4_4g23330 FvH4_5g25220 FvH4_5g30160 FvH4_5g32340 FvH4_5g37421 FvH4_6g21851 FvH4_6g21852 FvH4_6g22855 FvH4_6g22870 FvH4_6g30440 FvH4_6g30962 FvH4_6g37930
malus_domestica MD16G1158000.v1.1
prunus_persica Prupe.1G266000_v2.0.a1 Prupe.1G266100_v2.0.a1 Prupe.1G266200_v2.0.a1 Prupe.2G006600_v2.0.a1 Prupe.2G110300_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0322121 RchiOBHm_Chr1g0322131 RchiOBHm_Chr1g0341871 RchiOBHm_Chr1g0346721 RchiOBHm_Chr2g0118081 RchiOBHm_Chr2g0138651 RchiOBHm_Chr3g0468651 RchiOBHm_Chr3g0475981 RchiOBHm_Chr4g0416921 RchiOBHm_Chr5g0068021 RchiOBHm_Chr6g0273901 RchiOBHm_Chr6g0278661 RchiOBHm_Chr6g0281481 RchiOBHm_Chr6g0306081 RchiOBHm_Chr6g0306091 RchiOBHm_Chr7g0220731 RchiOBHm_Chr7g0220741 RchiOBHm_Chr7g0222641 RchiOBHm_Chr7g0225401 RchiOBHm_Chr7g0230481
rosa_laevigata RLG00000001569 RLG00000006184 RLG00000007906 RLG00000009863 RLG00000011359 RLG00000016125 RLG00000018543 RLG00000020693 RLG00000029658 RLG00000029716
rosa_multiflora Rmu_co8244591.1_g000001 Rmu_co8396391.1_g000001 Rmu_sc0000120.1_g000013 Rmu_sc0000239.1_g000047 Rmu_sc0000252.1_g000019 Rmu_sc0000322.1_g000037 Rmu_sc0000376.1_g000010 Rmu_sc0000435.1_g000033 Rmu_sc0001872.1_g000001 Rmu_sc0001992.1_g000011 Rmu_sc0001992.1_g000012 Rmu_sc0002231.1_g000010 Rmu_sc0002372.1_g000033 Rmu_sc0002705.1_g000012 Rmu_sc0003435.1_g000015 Rmu_sc0004084.1_g000042 Rmu_sc0004084.1_g000043 Rmu_sc0004755.1_g000011 Rmu_sc0005391.1_g000010 Rmu_sc0005970.1_g000001 Rmu_sc0006163.1_g000003 Rmu_sc0006422.1_g000004 Rmu_sc0006422.1_g000005 Rmu_sc0006952.1_g000002 Rmu_sc0010937.1_g000007 Rmu_sc0012130.1_g000008 Rmu_sc0024120.1_g000001 Rmu_sc0035618.1_g000001
rosa_roxburghii Rroxscaffold_1G00039230 Rroxscaffold_2G00085900 Rroxscaffold_2G00085910 Rroxscaffold_2G00125240 Rroxscaffold_2G00126850 Rroxscaffold_2G00150980 Rroxscaffold_3G00230580 Rroxscaffold_3G00245050 Rroxscaffold_4G00320680 Rroxscaffold_4G00328800 Rroxscaffold_5G00349640 Rroxscaffold_6G00406600 Rroxscaffold_7G00165570 Rroxscaffold_7G00191490 Rroxscaffold_7G00192700 Rroxscaffold_7G00192710 Rroxscaffold_7G00201190 Rroxscaffold_7G00201620
rosa_rugosa Rorug01G0033100 Rorug01G0089100 Rorug01G0102000 Rorug01G0353100 Rorug01G0353200 Rorug01G0353300 Rorug02G0276900 Rorug02G0362200 Rorug02G0445100 Rorug02G0627600 Rorug02G0627600 Rorug02G0627600 Rorug03G0267700 Rorug03G0267800 Rorug03G0327400 Rorug04G0004500 Rorug04G0006700 Rorug04G0061500 Rorug04G0064700 Rorug04G0073300 Rorug04G0094300 Rorug04G0181200 Rorug04G0181200 Rorug04G0330100 Rorug05G0035600 Rorug05G0038600 Rorug05G0201600 Rorug05G0367700 Rorug05G0407600 Rorug05G0407700 Rorug05G0543400 Rorug06G0012200 Rorug06G0363700.1 Rorug07G0085100
rosa_samantha Rh1AG049700 Rh1AG182700 Rh1AG182800 Rh1BG015500 Rh1BG398700 Rh1BG409100 Rh1CG074100 Rh1CG169200 Rh1CG169300 Rh1CG169400 Rh1DG130800 Rh1DG130900 Rh1DG440400 Rh2DG440900 Rh3AG144700 Rh3BG167100 Rh3DG366800 Rh4AG214400 Rh4BG189700 Rh4BG189800 Rh4CG268600 Rh4DG004400 Rh4DG147900 Rh4DG248300 Rh5AG053900 Rh5AG054000 Rh5AG317200 Rh5AG326400 Rh5AG332600 Rh5AG419700 Rh5CG284900 Rh5CG288600 Rh6AG252700 Rh6AG252800 Rh6AG256900 Rh6AG257000 Rh6AG257500 Rh6BG175300 Rh6DG226200 Rh6DG264500 Rh7AG374000 Rh7AG398100 Rh7BG274900 Rh7BG300500 Rh7BG308200 Rh7BG451200
rosa_wichuraiana Rw1G015860 Rw1G022160 Rw3G016160 Rw3G021170 Rw4G006020 Rw4G008740 Rw5G027400 Rw5G031840 Rw5G036940 Rw6G008320 Rw6G030330 Rw7G033450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 453
AccI GTMKAC 2 cut(s) 59, 217
AccII CGCG 1 cut(s) 317
AciI CCGC 7 cut(s) 115, 139, 142, 145, 162, 223, 226
AclWI GGATC 1 cut(s) 746
AcoI YGGCCR 1 cut(s) 890
AfaI GTAC 3 cut(s) 119, 294, 360
AfiI CCNNNNNNNGG 2 cut(s) 615, 676
AgeI ACCGGT 1 cut(s) 108
AgsI TTSAA 3 cut(s) 133, 494, 698
AjnI CCWGG 1 cut(s) 664
AjuI GAANNNNNNNTTGG 1 cut(s) 28
AluBI AGCT 8 cut(s) 54, 282, 313, 348, 379, 479, 545, 812
AluI AGCT 8 cut(s) 54, 282, 313, 348, 379, 479, 545, 812
Alw21I GWGCWC 5 cut(s) 284, 315, 350, 381, 411
AlwI GGATC 1 cut(s) 746
Ama87I CYCGRG 4 cut(s) 277, 308, 343, 374
AoxI GGCC 2 cut(s) 667, 890
ApeKI GCWGC 5 cut(s) 479, 680, 728, 764, 790
AsiGI ACCGGT 1 cut(s) 108
AspLEI GCGC 2 cut(s) 172, 319
AvaI CYCGRG 4 cut(s) 277, 308, 343, 374
BaeGI GKGCMC 1 cut(s) 592
BanII GRGCYC 4 cut(s) 284, 315, 350, 381
BbsI GAAGAC 1 cut(s) 588
Bbv12I GWGCWC 5 cut(s) 284, 315, 350, 381, 411
BbvCI CCTCAGC 1 cut(s) 546
BbvI GCAGC 5 cut(s) 466, 667, 715, 751, 802
BccI CCATC 4 cut(s) 261, 446, 617, 645
BciT130I CCWGG 1 cut(s) 666
BfaI CTAG 2 cut(s) 210, 483
BfmI CTRYAG 1 cut(s) 788
BisI GCNGC 9 cut(s) 140, 143, 224, 227, 480, 681, 729, 765, 791
BlsI GCNGC 9 cut(s) 141, 144, 225, 228, 481, 682, 730, 766, 792
Bme1390I CCNGG 1 cut(s) 666
BmeT110I CYCGRG 4 cut(s) 277, 308, 343, 374
BmrFI CCNGG 1 cut(s) 666
BmsI GCATC 4 cut(s) 328, 423, 631, 802
BpiI GAAGAC 1 cut(s) 588
BpmI CTGGAG 1 cut(s) 614
Bpu10I CCTNAGC 1 cut(s) 546
BsaAI YACGTR 1 cut(s) 806
BsaJI CCNNGG 1 cut(s) 987
BsaWI WCCGGW 1 cut(s) 108
BsaXI ACNNNNNCTCC 4 cut(s) 41, 71, 73, 103
Bsc4I CCNNNNNNNGG 2 cut(s) 615, 676
Bse118I RCCGGY 1 cut(s) 108
Bse1I ACTGG 2 cut(s) 160, 597
BseBI CCWGG 1 cut(s) 666
BseDI CCNNGG 1 cut(s) 987
BseGI GGATG 2 cut(s) 446, 637
BseLI CCNNNNNNNGG 2 cut(s) 615, 676
BseMII CTCAG 1 cut(s) 537
BseNI ACTGG 2 cut(s) 160, 597
BseRI GAGGAG 1 cut(s) 97
BseSI GKGCMC 1 cut(s) 592
BseXI GCAGC 5 cut(s) 466, 667, 715, 751, 802
BsgI GTGCAG 1 cut(s) 579
Bsh1236I CGCG 1 cut(s) 317
BshFI GGCC 2 cut(s) 669, 892
BshTI ACCGGT 1 cut(s) 108
BsiHKAI GWGCWC 5 cut(s) 284, 315, 350, 381, 411
BsiHKCI CYCGRG 4 cut(s) 277, 308, 343, 374
BsiSI CCGG 1 cut(s) 109
BslFI GGGAC 1 cut(s) 532
BslI CCNNNNNNNGG 2 cut(s) 615, 676
BsmFI GGGAC 1 cut(s) 532
BsnI GGCC 2 cut(s) 669, 892
BsoBI CYCGRG 4 cut(s) 277, 308, 343, 374
Bsp1286I GDGCHC 6 cut(s) 284, 315, 350, 381, 411, 592
Bsp143I GATC 1 cut(s) 751
BspACI CCGC 7 cut(s) 115, 139, 142, 145, 162, 223, 226
BspANI GGCC 2 cut(s) 669, 892
BspCNI CTCAG 1 cut(s) 538
BspFNI CGCG 1 cut(s) 317
BspMAI CTGCAG 1 cut(s) 792
BspPI GGATC 1 cut(s) 746
BspQI GCTCTTC 2 cut(s) 273, 339
BsrFI RCCGGY 1 cut(s) 108
BsrI ACTGG 2 cut(s) 160, 597
BssAI RCCGGY 1 cut(s) 108
BssECI CCNNGG 1 cut(s) 987
BssMI GATC 1 cut(s) 751
Bst2UI CCWGG 1 cut(s) 666
Bst4CI ACNGT 5 cut(s) 42, 64, 127, 523, 831
Bst6I CTCTTC 4 cut(s) 273, 304, 329, 339
BstBAI YACGTR 1 cut(s) 806
BstC8I GCNNGC 5 cut(s) 174, 284, 315, 350, 381
BstDEI CTNAG 3 cut(s) 546, 671, 820
BstDSI CCRYGG 1 cut(s) 987
BstF5I GGATG 2 cut(s) 446, 637
BstFNI CGCG 1 cut(s) 317
BstHHI GCGC 2 cut(s) 172, 319
BstKTI GATC 1 cut(s) 754
BstMBI GATC 1 cut(s) 751
BstMWI GCNNNNNNNGC 1 cut(s) 159
BstNI CCWGG 1 cut(s) 666
BstNSI RCATGY 1 cut(s) 602
BstSCI CCNGG 1 cut(s) 664
BstSFI CTRYAG 1 cut(s) 788
BstSLI GKGCMC 1 cut(s) 592
BstUI CGCG 1 cut(s) 317
BstV1I GCAGC 5 cut(s) 466, 667, 715, 751, 802
BstV2I GAAGAC 1 cut(s) 588
BstX2I RGATCY 1 cut(s) 751
BstXI CCANNNNNNTGG 1 cut(s) 644
BstYI RGATCY 1 cut(s) 751
BsuRI GGCC 2 cut(s) 669, 892
BtgI CCRYGG 1 cut(s) 987
BtsCI GGATG 2 cut(s) 446, 637
BtsI GCAGTG 2 cut(s) 183, 868
BtsIMutI CAGTG 5 cut(s) 153, 183, 590, 610, 868
Cac8I GCNNGC 5 cut(s) 174, 284, 315, 350, 381
CfoI GCGC 2 cut(s) 172, 319
Cfr10I RCCGGY 1 cut(s) 108
Csp6I GTAC 3 cut(s) 118, 293, 359
CspAI ACCGGT 1 cut(s) 108
CviAII CATG 7 cut(s) 329, 437, 449, 458, 466, 599, 853
CviQI GTAC 3 cut(s) 118, 293, 359
DdeI CTNAG 3 cut(s) 546, 671, 820
DpnI GATC 1 cut(s) 753
DpnII GATC 1 cut(s) 751
DrdI GACNNNNNNGTC 1 cut(s) 453
DseDI GACNNNNNNGTC 1 cut(s) 453
EaeI YGGCCR 1 cut(s) 890
Eam1104I CTCTTC 4 cut(s) 273, 304, 329, 339
EarI CTCTTC 4 cut(s) 273, 304, 329, 339
Ecl136II GAGCTC 4 cut(s) 282, 313, 348, 379
Eco24I GRGCYC 4 cut(s) 284, 315, 350, 381
Eco53kI GAGCTC 4 cut(s) 282, 313, 348, 379
Eco88I CYCGRG 4 cut(s) 277, 308, 343, 374
EcoICRI GAGCTC 4 cut(s) 282, 313, 348, 379
EcoRII CCWGG 1 cut(s) 664
EcoT22I ATGCAT 1 cut(s) 438
EcoT38I GRGCYC 4 cut(s) 284, 315, 350, 381
FaeI CATG 7 cut(s) 332, 440, 452, 461, 469, 602, 856
FaiI YATR 7 cut(s) 330, 438, 450, 459, 467, 600, 854
FalI AAGNNNNNCTT 4 cut(s) 38, 70, 347, 379
FaqI GGGAC 1 cut(s) 532
FatI CATG 7 cut(s) 328, 436, 448, 457, 465, 598, 852
FauI CCCGC 2 cut(s) 138, 155
FblI GTMKAC 2 cut(s) 59, 217
Fnu4HI GCNGC 9 cut(s) 140, 143, 224, 227, 480, 681, 729, 765, 791
FokI GGATG 2 cut(s) 453, 624
FriOI GRGCYC 4 cut(s) 284, 315, 350, 381
Fsp4HI GCNGC 9 cut(s) 140, 143, 224, 227, 480, 681, 729, 765, 791
FspBI CTAG 2 cut(s) 210, 483
GlaI GCGC 2 cut(s) 171, 318
GluI GCNGC 9 cut(s) 140, 143, 224, 227, 480, 681, 729, 765, 791
GsuI CTGGAG 1 cut(s) 614
HaeIII GGCC 2 cut(s) 669, 892
HapII CCGG 1 cut(s) 109
HhaI GCGC 2 cut(s) 172, 319
Hin1II CATG 7 cut(s) 332, 440, 452, 461, 469, 602, 856
Hin6I GCGC 2 cut(s) 170, 317
HinP1I GCGC 2 cut(s) 170, 317
HincII GTYRAC 3 cut(s) 60, 218, 240
HindII GTYRAC 3 cut(s) 60, 218, 240
HinfI GANTC 2 cut(s) 204, 824
HpaII CCGG 1 cut(s) 109
Hpy166II GTNNAC 5 cut(s) 60, 218, 240, 446, 488
Hpy188I TCNGA 2 cut(s) 106, 634
Hpy188III TCNNGA 4 cut(s) 19, 79, 210, 494
Hpy8I GTNNAC 5 cut(s) 60, 218, 240, 446, 488
Hpy99I CGWCG 9 cut(s) 64, 222, 848, 879, 912, 927, 938, 957, 978
HpyAV CCTTC 6 cut(s) 40, 283, 349, 380, 545, 709
HpyCH4III ACNGT 5 cut(s) 42, 64, 127, 523, 831
HpyCH4IV ACGT 1 cut(s) 805
HpyCH4V TGCA 7 cut(s) 153, 425, 436, 560, 622, 731, 790
HpyF10VI GCNNNNNNNGC 1 cut(s) 159
HpyF3I CTNAG 3 cut(s) 546, 671, 820
HpySE526I ACGT 1 cut(s) 805
Hsp92II CATG 7 cut(s) 332, 440, 452, 461, 469, 602, 856
HspAI GCGC 2 cut(s) 170, 317
Kzo9I GATC 1 cut(s) 751
LguI GCTCTTC 2 cut(s) 273, 339
LmnI GCTCC 4 cut(s) 406, 476, 563, 595
Lsp1109I GCAGC 5 cut(s) 466, 667, 715, 751, 802
LweI GCATC 4 cut(s) 328, 423, 631, 802
MaeI CTAG 2 cut(s) 210, 483
MaeII ACGT 1 cut(s) 805
MaeIII GTNAC 1 cut(s) 859
MalI GATC 1 cut(s) 753
MboI GATC 1 cut(s) 751
MboII GAAGA 8 cut(s) 177, 224, 260, 291, 316, 326, 442, 593
MflI RGATCY 1 cut(s) 751
MhlI GDGCHC 6 cut(s) 284, 315, 350, 381, 411, 592
MluCI AATT 4 cut(s) 5, 133, 509, 732
MmeI TCCRAC 1 cut(s) 562
MnlI CCTC 7 cut(s) 75, 80, 206, 541, 548, 667, 909
Mph1103I ATGCAT 1 cut(s) 438
MslI CAYNNNNRTG 3 cut(s) 441, 603, 857
MspA1I CMGCKG 1 cut(s) 545
MspI CCGG 1 cut(s) 109
MspR9I CCNGG 1 cut(s) 666
MvaI CCWGG 1 cut(s) 666
MvnI CGCG 1 cut(s) 317
MwoI GCNNNNNNNGC 1 cut(s) 159
NdeII GATC 1 cut(s) 751
NlaIII CATG 7 cut(s) 332, 440, 452, 461, 469, 602, 856
NmuCI GTSAC 1 cut(s) 859
NsiI ATGCAT 1 cut(s) 438
NspI RCATGY 1 cut(s) 602
PaeR7I CTCGAG 4 cut(s) 277, 308, 343, 374
PciSI GCTCTTC 2 cut(s) 273, 339
PcsI WCGNNNNNNNCGW 2 cut(s) 194, 936
PfeI GAWTC 2 cut(s) 204, 824
PinAI ACCGGT 1 cut(s) 108
PkrI GCNGC 9 cut(s) 141, 144, 225, 228, 481, 682, 730, 766, 792
Ppu21I YACGTR 1 cut(s) 806
Psp124BI GAGCTC 4 cut(s) 284, 315, 350, 381
Psp6I CCWGG 1 cut(s) 664
PspGI CCWGG 1 cut(s) 664
PspXI VCTCGAGB 4 cut(s) 277, 308, 343, 374
PstI CTGCAG 1 cut(s) 792
PsuI RGATCY 1 cut(s) 751
PvuII CAGCTG 1 cut(s) 545
RsaI GTAC 3 cut(s) 119, 294, 360
RsaNI GTAC 3 cut(s) 118, 293, 359
RseI CAYNNNNRTG 3 cut(s) 441, 603, 857
SacI GAGCTC 4 cut(s) 284, 315, 350, 381
SalI GTCGAC 2 cut(s) 58, 216
SapI GCTCTTC 2 cut(s) 273, 339
SatI GCNGC 9 cut(s) 140, 143, 224, 227, 480, 681, 729, 765, 791
Sau3AI GATC 1 cut(s) 751
ScrFI CCNGG 1 cut(s) 666
SduI GDGCHC 6 cut(s) 284, 315, 350, 381, 411, 592
SfaNI GCATC 4 cut(s) 328, 423, 631, 802
SfcI CTRYAG 1 cut(s) 788
Sfr274I CTCGAG 4 cut(s) 277, 308, 343, 374
SlaI CTCGAG 4 cut(s) 277, 308, 343, 374
SmiMI CAYNNNNRTG 3 cut(s) 441, 603, 857
SmlI CTYRAG 4 cut(s) 277, 308, 343, 374
SmoI CTYRAG 4 cut(s) 277, 308, 343, 374
Sse9I AATT 4 cut(s) 5, 133, 509, 732
SsiI CCGC 7 cut(s) 115, 139, 142, 145, 162, 223, 226
SspMI CTAG 2 cut(s) 210, 483
SstI GAGCTC 4 cut(s) 284, 315, 350, 381
StyD4I CCNGG 1 cut(s) 664
TaaI ACNGT 5 cut(s) 42, 64, 127, 523, 831
TaiI ACGT 1 cut(s) 808
TaqI TCGA 8 cut(s) 37, 59, 202, 217, 278, 309, 344, 375
TaqII GACCGA 2 cut(s) 178, 225
TasI AATT 4 cut(s) 5, 133, 509, 732
TauI GCSGC 4 cut(s) 142, 145, 226, 229
TfiI GAWTC 2 cut(s) 204, 824
TscAI CASTG 5 cut(s) 160, 183, 597, 610, 875
TseFI GTSAC 1 cut(s) 859
TseI GCWGC 5 cut(s) 479, 680, 728, 764, 790
Tsp45I GTSAC 1 cut(s) 859
TspDTI ATGAA 2 cut(s) 17, 90
TspRI CASTG 5 cut(s) 160, 183, 597, 610, 875
XbaI TCTAGA 1 cut(s) 209
XceI RCATGY 1 cut(s) 602
XhoI CTCGAG 4 cut(s) 277, 308, 343, 374
XmiI GTMKAC 2 cut(s) 59, 217
XspI CTAG 2 cut(s) 210, 483
Zsp2I ATGCAT 1 cut(s) 438
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.