Rw5G036940

Transposase family tnp2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000136
Physical Location & Seq
Reverse (-)
64944139 .. 64948791
4653 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G036940.1

Sequence Viewer

Length: 1494 bp
ATGCATATAGAGAAGAATGTGTGTGACAATGTTCTTGGGACCATACTAGGAGTTGCTGGAAAATCAAAGGACAATATCAGTTCTCGACGTGATCTTGAGCTTATGGGAATTAGAGAGCAGTACCATGTGAAGAGGAGGGAGTCCGGTTCAGAATACTTTGATCCTGCAGATTTTGAGATGAATAATGATGGGAAAGATAAGTTCCTCGTAGCCTTATCAGAAACAAGAATGCCGGATGGTTCTGCTTCTCATATTGCACGGCGGGTACGTTTGAAAGATCGAAGTATCAGCGGTCTTAAAAGTCATGACAATCATATTCTTTTACAGCAATTGATTCCATTATCTATACGAAGTTCTTTACCGAAGAAAGTGGTCGAAGGGCTGATTGATCTGGGCAAGTTTTTCAAAGTATTGTGCTCCAAACTAAATTGTGCAGCAGATTTGGAAGAGGCACGTTCTCGTATAGTGGTGACCCTTTGTGATCTTGAAAAGATATTTCCACCATCATTTTTTGATGTGATGGAGCATTTACCTATCCATCTAGTCCAAGAAGCATTAATTGCTGGTGCTGTGCAGTTTCGGTGGATGTACCCCATTGAGAGGTATCTGTTGACTTTAAAGGAATATGTACGTAACAGAGCTTGTCCGGAAGCATCAATAGCTAAAGGTTATCTAATGGAAGAATGCATGAATTTCTGTACCCAATATCTTAAAGATGTGGAGAGTAAGTCAAACAGACCGTTAAGGAGAAAGAATCGTGATGACCATGAGAAAGGAAATTGTGTTCTCAGTGAAGTTACGCGCATACAAATTCATCGATGGGTCTTGTTTCATACAAGGGCAGTCACGCCATTTCTCAAGGAACATCTTACTGCCATTAAACAACAATTTCCGGATGCAGATGACCATTATGTTCAACGTGTTCATTTTGATGAGTTTGCGAACTGGTTTAAGGATCATGTGACCATGAAGAAAAGAAAGGTTGGGAGACCACGCACTATGTCTGAGTTTTTGACTTCAAGTGGATCTAATTCTTCAAACAGCAATGGTTCCTCATCGCAGCCATCCTATAGGTCTACTAATCCACCTCCTACACCCCCACTGCCTCCACCCCCACCGCCTCCTTCTGCACCTTCTCCTCCAATATTACCTCTTCCATCTCTAGAAGCTGAAGTACAATCTGCTACCTTTATTACTAGAGGTCACAATAAAGGTATCCCAGAGTGGAATACTGGAGTTAAAATCAAGATTATGTTTGATTCTAACTTCCAACCTGTTGGAGAAAGAGCTACACAGCTAAAGTCACAATTGGGGCAAATTGTACGCGATGGCCAGAGGATTCCACTAACATTACTTGACTGGAAGGCTGTTGGACCTGATGTGAAAGAGGGAATATGGAAAGAGGTTCAGGATAAAATCCCAGATTTTGAATTCAAACTACATGTGATAGTATCTGATCTATTCATGTTTCATTTTCATGTGAATCCGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4216 DUF4218 Transpos_assoc Transposase_21

Protein Analysis

497

Amino Acids

56.68

Weight (kDa)

8.92

Isoelectric Point (pI)

51.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4218 PF13960 138 - 249 4.9e-44 Domain of unknown function (DUF4218)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000136)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g28181 FvH4_1g28182 FvH4_1g28940 FvH4_1g29801 FvH4_1g29802 FvH4_3g03321 FvH4_3g12341 FvH4_3g22002 FvH4_3g22003 FvH4_3g22852 FvH4_3g22860 FvH4_3g32806 FvH4_4g02410 FvH4_4g08110 FvH4_4g09791 FvH4_4g09792 FvH4_4g09830 FvH4_4g09831 FvH4_4g09832 FvH4_4g23330 FvH4_5g25220 FvH4_5g30160 FvH4_5g32340 FvH4_5g37421 FvH4_6g21851 FvH4_6g21852 FvH4_6g22855 FvH4_6g22870 FvH4_6g30440 FvH4_6g30962 FvH4_6g37930
malus_domestica MD16G1158000.v1.1
prunus_persica Prupe.1G266000_v2.0.a1 Prupe.1G266100_v2.0.a1 Prupe.1G266200_v2.0.a1 Prupe.2G006600_v2.0.a1 Prupe.2G110300_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0322121 RchiOBHm_Chr1g0322131 RchiOBHm_Chr1g0341871 RchiOBHm_Chr1g0346721 RchiOBHm_Chr2g0118081 RchiOBHm_Chr2g0138651 RchiOBHm_Chr3g0468651 RchiOBHm_Chr3g0475981 RchiOBHm_Chr4g0416921 RchiOBHm_Chr5g0068021 RchiOBHm_Chr6g0273901 RchiOBHm_Chr6g0278661 RchiOBHm_Chr6g0281481 RchiOBHm_Chr6g0306081 RchiOBHm_Chr6g0306091 RchiOBHm_Chr7g0220731 RchiOBHm_Chr7g0220741 RchiOBHm_Chr7g0222641 RchiOBHm_Chr7g0225401 RchiOBHm_Chr7g0230481
rosa_laevigata RLG00000001569 RLG00000006184 RLG00000007906 RLG00000009863 RLG00000011359 RLG00000016125 RLG00000018543 RLG00000020693 RLG00000029658 RLG00000029716
rosa_multiflora Rmu_co8244591.1_g000001 Rmu_co8396391.1_g000001 Rmu_sc0000120.1_g000013 Rmu_sc0000239.1_g000047 Rmu_sc0000252.1_g000019 Rmu_sc0000322.1_g000037 Rmu_sc0000376.1_g000010 Rmu_sc0000435.1_g000033 Rmu_sc0001872.1_g000001 Rmu_sc0001992.1_g000011 Rmu_sc0001992.1_g000012 Rmu_sc0002231.1_g000010 Rmu_sc0002372.1_g000033 Rmu_sc0002705.1_g000012 Rmu_sc0003435.1_g000015 Rmu_sc0004084.1_g000042 Rmu_sc0004084.1_g000043 Rmu_sc0004755.1_g000011 Rmu_sc0005391.1_g000010 Rmu_sc0005970.1_g000001 Rmu_sc0006163.1_g000003 Rmu_sc0006422.1_g000004 Rmu_sc0006422.1_g000005 Rmu_sc0006952.1_g000002 Rmu_sc0010937.1_g000007 Rmu_sc0012130.1_g000008 Rmu_sc0024120.1_g000001 Rmu_sc0035618.1_g000001
rosa_roxburghii Rroxscaffold_1G00039230 Rroxscaffold_2G00085900 Rroxscaffold_2G00085910 Rroxscaffold_2G00125240 Rroxscaffold_2G00126850 Rroxscaffold_2G00150980 Rroxscaffold_3G00230580 Rroxscaffold_3G00245050 Rroxscaffold_4G00320680 Rroxscaffold_4G00328800 Rroxscaffold_5G00349640 Rroxscaffold_6G00406600 Rroxscaffold_7G00165570 Rroxscaffold_7G00191490 Rroxscaffold_7G00192700 Rroxscaffold_7G00192710 Rroxscaffold_7G00201190 Rroxscaffold_7G00201620
rosa_rugosa Rorug01G0033100 Rorug01G0089100 Rorug01G0102000 Rorug01G0353100 Rorug01G0353200 Rorug01G0353300 Rorug02G0276900 Rorug02G0362200 Rorug02G0445100 Rorug02G0627600 Rorug02G0627600 Rorug02G0627600 Rorug03G0267700 Rorug03G0267800 Rorug03G0327400 Rorug04G0004500 Rorug04G0006700 Rorug04G0061500 Rorug04G0064700 Rorug04G0073300 Rorug04G0094300 Rorug04G0181200 Rorug04G0181200 Rorug04G0330100 Rorug05G0035600 Rorug05G0038600 Rorug05G0201600 Rorug05G0367700 Rorug05G0407600 Rorug05G0407700 Rorug05G0543400 Rorug06G0012200 Rorug06G0363700.1 Rorug07G0085100
rosa_samantha Rh1AG049700 Rh1AG182700 Rh1AG182800 Rh1BG015500 Rh1BG398700 Rh1BG409100 Rh1CG074100 Rh1CG169200 Rh1CG169300 Rh1CG169400 Rh1DG130800 Rh1DG130900 Rh1DG440400 Rh2DG440900 Rh3AG144700 Rh3BG167100 Rh3DG366800 Rh4AG214400 Rh4BG189700 Rh4BG189800 Rh4CG268600 Rh4DG004400 Rh4DG147900 Rh4DG248300 Rh5AG053900 Rh5AG054000 Rh5AG317200 Rh5AG326400 Rh5AG332600 Rh5AG419700 Rh5CG284900 Rh5CG288600 Rh6AG252700 Rh6AG252800 Rh6AG256900 Rh6AG257000 Rh6AG257500 Rh6BG175300 Rh6DG226200 Rh6DG264500 Rh7AG374000 Rh7AG398100 Rh7BG274900 Rh7BG300500 Rh7BG308200 Rh7BG451200
rosa_wichuraiana Rw1G015860 Rw1G022160 Rw3G016160 Rw3G021170 Rw4G006020 Rw4G008740 Rw5G027400 Rw5G031840 Rw5G036940 Rw6G008320 Rw6G030330 Rw7G033450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1076
AccII CGCG 2 cut(s) 802, 1326
AccIII TCCGGA 2 cut(s) 646, 892
AciI CCGC 3 cut(s) 262, 291, 1118
AclWI GGATC 3 cut(s) 155, 963, 1033
AcoI YGGCCR 1 cut(s) 1330
AcsI RAATTY 3 cut(s) 691, 810, 1430
AcuI CTGAAG 1 cut(s) 1191
AfaI GTAC 7 cut(s) 122, 267, 590, 630, 700, 1176, 1323
AfiI CCNNNNNNNGG 1 cut(s) 600
AflIII ACRYGT 2 cut(s) 919, 1441
AgsI TTSAA 8 cut(s) 274, 406, 488, 917, 1020, 1038, 1430, 1435
AjiI CACGTC 1 cut(s) 89
AloI GAACNNNNNNTCC 2 cut(s) 768, 800
AluBI AGCT 6 cut(s) 100, 641, 662, 1169, 1289, 1297
AluI AGCT 6 cut(s) 100, 641, 662, 1169, 1289, 1297
Alw21I GWGCWC 1 cut(s) 419
Alw26I GTCTC 1 cut(s) 982
AlwI GGATC 3 cut(s) 155, 963, 1033
Aor13HI TCCGGA 2 cut(s) 646, 892
AoxI GGCC 1 cut(s) 1330
ApeKI GCWGC 2 cut(s) 434, 1060
ApoI RAATTY 3 cut(s) 691, 810, 1430
AseI ATTAAT 1 cut(s) 557
AspLEI GCGC 1 cut(s) 804
AspS9I GGNCC 2 cut(s) 39, 1373
AsuHPI GGTGA 1 cut(s) 481
AvaII GGWCC 2 cut(s) 39, 1373
BalI TGGCCA 1 cut(s) 1332
BarI GAAGNNNNNNTAC 2 cut(s) 1158, 1190
Bbv12I GWGCWC 1 cut(s) 419
BbvI GCAGC 2 cut(s) 446, 1072
BccI CCATC 9 cut(s) 182, 230, 511, 514, 546, 813, 1072, 1165, 1322
BceAI ACGGC 1 cut(s) 275
BciVI GTATCC 1 cut(s) 1226
BcoDI GTCTC 1 cut(s) 982
BfaI CTAG 4 cut(s) 47, 542, 1163, 1197
BfmI CTRYAG 2 cut(s) 165, 1069
BfuI GTATCC 1 cut(s) 1226
BisI GCNGC 2 cut(s) 435, 1061
BlsI GCNGC 2 cut(s) 436, 1062
Bme18I GGWCC 2 cut(s) 39, 1373
BmgBI CACGTC 1 cut(s) 89
BmgT120I GGNCC 2 cut(s) 39, 1373
BmiI GGNNCC 2 cut(s) 40, 1051
BmsI GCATC 2 cut(s) 662, 886
BpmI CTGGAG 1 cut(s) 1254
BpuEI CTTGAG 2 cut(s) 116, 842
Bsa29I ATCGAT 1 cut(s) 817
BsaAI YACGTR 1 cut(s) 632
BsaI GGTCTC 1 cut(s) 982
BsaWI WCCGGW 4 cut(s) 143, 646, 892, 1486
BsaXI ACNNNNNCTCC 2 cut(s) 713, 743
Bsc4I CCNNNNNNNGG 1 cut(s) 600
Bse1I ACTGG 3 cut(s) 950, 1237, 1364
Bse3DI GCAATG 1 cut(s) 1051
BseAI TCCGGA 2 cut(s) 646, 892
BseCI ATCGAT 1 cut(s) 817
BseGI GGATG 4 cut(s) 241, 591, 901, 1064
BseLI CCNNNNNNNGG 1 cut(s) 600
BseMI GCAATG 1 cut(s) 1051
BseMII CTCAG 2 cut(s) 802, 996
BseNI ACTGG 3 cut(s) 950, 1237, 1364
BseRI GAGGAG 2 cut(s) 148, 1128
BseXI GCAGC 2 cut(s) 446, 1072
BsgI GTGCAG 3 cut(s) 453, 593, 1113
Bsh1236I CGCG 2 cut(s) 802, 1326
BshFI GGCC 1 cut(s) 1332
BshVI ATCGAT 1 cut(s) 817
BsiHKAI GWGCWC 1 cut(s) 419
BsiSI CCGG 5 cut(s) 144, 233, 647, 893, 1487
BslFI GGGAC 1 cut(s) 52
BslI CCNNNNNNNGG 1 cut(s) 600
BsmAI GTCTC 1 cut(s) 982
BsmFI GGGAC 1 cut(s) 52
BsmI GAATGC 2 cut(s) 234, 689
BsnI GGCC 1 cut(s) 1332
Bso31I GGTCTC 1 cut(s) 982
Bsp1286I GDGCHC 1 cut(s) 419
Bsp13I TCCGGA 2 cut(s) 646, 892
Bsp143I GATC 8 cut(s) 91, 160, 277, 388, 481, 955, 1025, 1456
BspACI CCGC 3 cut(s) 262, 291, 1118
BspANI GGCC 1 cut(s) 1332
BspCNI CTCAG 2 cut(s) 801, 997
BspDI ATCGAT 1 cut(s) 817
BspEI TCCGGA 2 cut(s) 646, 892
BspFNI CGCG 2 cut(s) 802, 1326
BspHI TCATGA 1 cut(s) 304
BspLI GGNNCC 2 cut(s) 40, 1051
BspMAI CTGCAG 1 cut(s) 169
BspPI GGATC 3 cut(s) 155, 963, 1033
BspTNI GGTCTC 1 cut(s) 982
BsrDI GCAATG 1 cut(s) 1051
BsrI ACTGG 3 cut(s) 950, 1237, 1364
BssMI GATC 8 cut(s) 91, 160, 277, 388, 481, 955, 1025, 1456
Bst4CI ACNGT 1 cut(s) 741
Bst6I CTCTTC 3 cut(s) 125, 441, 1158
BstAPI GCANNNNNTGC 1 cut(s) 560
BstBAI YACGTR 1 cut(s) 632
BstDEI CTNAG 2 cut(s) 788, 1005
BstEII GGTNACC 1 cut(s) 469
BstF5I GGATG 4 cut(s) 241, 591, 901, 1064
BstFNI CGCG 2 cut(s) 802, 1326
BstHHI GCGC 1 cut(s) 804
BstKTI GATC 8 cut(s) 94, 163, 280, 391, 484, 958, 1028, 1459
BstMAI GTCTC 1 cut(s) 982
BstMBI GATC 8 cut(s) 91, 160, 277, 388, 481, 955, 1025, 1456
BstMWI GCNNNNNNNGC 2 cut(s) 560, 659
BstNSI RCATGY 1 cut(s) 1445
BstPI GGTNACC 1 cut(s) 469
BstSFI CTRYAG 2 cut(s) 165, 1069
BstSNI TACGTA 1 cut(s) 632
BstUI CGCG 2 cut(s) 802, 1326
BstV1I GCAGC 2 cut(s) 446, 1072
BstX2I RGATCY 1 cut(s) 1025
BstXI CCANNNNNNTGG 1 cut(s) 1277
BstYI RGATCY 1 cut(s) 1025
Bsu15I ATCGAT 1 cut(s) 817
BsuI GTATCC 1 cut(s) 1226
BsuRI GGCC 1 cut(s) 1332
BsuTUI ATCGAT 1 cut(s) 817
BtgZI GCGATG 2 cut(s) 1041, 1341
BtrI CACGTC 1 cut(s) 89
BtsCI GGATG 4 cut(s) 241, 591, 901, 1064
BtsI GCAGTG 1 cut(s) 1100
BtsIMutI CAGTG 2 cut(s) 796, 1100
CciI TCATGA 1 cut(s) 304
CfoI GCGC 1 cut(s) 804
Cfr13I GGNCC 2 cut(s) 39, 1373
ClaI ATCGAT 1 cut(s) 817
Csp6I GTAC 7 cut(s) 121, 266, 589, 629, 699, 1175, 1322
CviAII CATG 9 cut(s) 125, 305, 688, 767, 959, 967, 1442, 1465, 1478
CviQI GTAC 7 cut(s) 121, 266, 589, 629, 699, 1175, 1322
DdeI CTNAG 2 cut(s) 788, 1005
DpnI GATC 8 cut(s) 93, 162, 279, 390, 483, 957, 1027, 1458
DpnII GATC 8 cut(s) 91, 160, 277, 388, 481, 955, 1025, 1456
DraI TTTAAA 1 cut(s) 618
EaeI YGGCCR 1 cut(s) 1330
Eam1104I CTCTTC 3 cut(s) 125, 441, 1158
EarI CTCTTC 3 cut(s) 125, 441, 1158
Eco105I TACGTA 1 cut(s) 632
Eco31I GGTCTC 1 cut(s) 982
Eco47I GGWCC 2 cut(s) 39, 1373
Eco57I CTGAAG 1 cut(s) 1191
Eco91I GGTNACC 1 cut(s) 469
EcoO65I GGTNACC 1 cut(s) 469
EcoRI GAATTC 1 cut(s) 1430
EcoT22I ATGCAT 2 cut(s) 6, 689
FaeI CATG 9 cut(s) 128, 308, 691, 770, 962, 970, 1445, 1468, 1481
FaqI GGGAC 1 cut(s) 52
FatI CATG 9 cut(s) 124, 304, 687, 766, 958, 966, 1441, 1464, 1477
FauI CCCGC 1 cut(s) 255
FblI GTMKAC 1 cut(s) 1076
Fnu4HI GCNGC 2 cut(s) 435, 1061
FokI GGATG 4 cut(s) 248, 598, 908, 1051
Fsp4HI GCNGC 2 cut(s) 435, 1061
FspBI CTAG 4 cut(s) 47, 542, 1163, 1197
GlaI GCGC 1 cut(s) 803
GluI GCNGC 2 cut(s) 435, 1061
GsuI CTGGAG 1 cut(s) 1254
HaeIII GGCC 1 cut(s) 1332
HapII CCGG 5 cut(s) 144, 233, 647, 893, 1487
HhaI GCGC 1 cut(s) 804
Hin1II CATG 9 cut(s) 128, 308, 691, 770, 962, 970, 1445, 1468, 1481
Hin6I GCGC 1 cut(s) 802
HinP1I GCGC 1 cut(s) 802
HincII GTYRAC 1 cut(s) 612
HindII GTYRAC 1 cut(s) 612
HinfI GANTC 6 cut(s) 140, 334, 754, 1259, 1339, 1483
HpaII CCGG 5 cut(s) 144, 233, 647, 893, 1487
HphI GGTGA 1 cut(s) 481
Hpy166II GTNNAC 2 cut(s) 612, 1077
Hpy188I TCNGA 4 cut(s) 151, 220, 1006, 1456
Hpy8I GTNNAC 2 cut(s) 612, 1077
Hpy99I CGWCG 1 cut(s) 90
HpyAV CCTTC 4 cut(s) 371, 1134, 1143, 1357
HpyCH4III ACNGT 1 cut(s) 741
HpyCH4IV ACGT 5 cut(s) 88, 268, 454, 631, 919
HpyCH4V TGCA 8 cut(s) 4, 167, 257, 434, 574, 687, 899, 1130
HpyF10VI GCNNNNNNNGC 2 cut(s) 560, 659
HpyF3I CTNAG 2 cut(s) 788, 1005
HpySE526I ACGT 5 cut(s) 88, 268, 454, 631, 919
Hsp92II CATG 9 cut(s) 128, 308, 691, 770, 962, 970, 1445, 1468, 1481
HspAI GCGC 1 cut(s) 802
Kpn2I TCCGGA 2 cut(s) 646, 892
Kzo9I GATC 8 cut(s) 91, 160, 277, 388, 481, 955, 1025, 1456
LmnI GCTCC 2 cut(s) 422, 523
Lsp1109I GCAGC 2 cut(s) 446, 1072
LweI GCATC 2 cut(s) 662, 886
MaeI CTAG 4 cut(s) 47, 542, 1163, 1197
MaeII ACGT 5 cut(s) 88, 268, 454, 631, 919
MaeIII GTNAC 8 cut(s) 23, 469, 632, 796, 844, 961, 1202, 1302
MalI GATC 8 cut(s) 93, 162, 279, 390, 483, 957, 1027, 1458
MboI GATC 8 cut(s) 91, 160, 277, 388, 481, 955, 1025, 1456
MboII GAAGA 8 cut(s) 25, 142, 376, 458, 692, 982, 1026, 1145
MfeI CAATTG 2 cut(s) 329, 1307
MflI RGATCY 1 cut(s) 1025
MhlI GDGCHC 1 cut(s) 419
MlsI TGGCCA 1 cut(s) 1332
MluNI TGGCCA 1 cut(s) 1332
MlyI GAGTC 1 cut(s) 149
MmeI TCCRAC 3 cut(s) 1258, 1294, 1351
Mox20I TGGCCA 1 cut(s) 1332
Mph1103I ATGCAT 2 cut(s) 6, 689
MroI TCCGGA 2 cut(s) 646, 892
MscI TGGCCA 1 cut(s) 1332
MseI TTAA 8 cut(s) 297, 557, 617, 711, 743, 879, 951, 1239
MslI CAYNNNNRTG 2 cut(s) 930, 1476
Msp20I TGGCCA 1 cut(s) 1332
MspA1I CMGCKG 1 cut(s) 291
MspI CCGG 5 cut(s) 144, 233, 647, 893, 1487
MunI CAATTG 2 cut(s) 329, 1307
Mva1269I GAATGC 2 cut(s) 234, 689
MvnI CGCG 2 cut(s) 802, 1326
MwoI GCNNNNNNNGC 2 cut(s) 560, 659
NdeII GATC 8 cut(s) 91, 160, 277, 388, 481, 955, 1025, 1456
NlaIII CATG 9 cut(s) 128, 308, 691, 770, 962, 970, 1445, 1468, 1481
NlaIV GGNNCC 2 cut(s) 40, 1051
NmuCI GTSAC 6 cut(s) 23, 469, 844, 961, 1202, 1302
NsiI ATGCAT 2 cut(s) 6, 689
NspI RCATGY 1 cut(s) 1445
PagI TCATGA 1 cut(s) 304
PciI ACATGT 1 cut(s) 1441
PcsI WCGNNNNNNNCGW 1 cut(s) 265
PctI GAATGC 2 cut(s) 234, 689
PfeI GAWTC 5 cut(s) 334, 754, 1259, 1339, 1483
PkrI GCNGC 2 cut(s) 436, 1062
PleI GAGTC 1 cut(s) 148
PpsI GAGTC 1 cut(s) 148
Ppu21I YACGTR 1 cut(s) 632
PscI ACATGT 1 cut(s) 1441
PshBI ATTAAT 1 cut(s) 557
PspEI GGTNACC 1 cut(s) 469
PspN4I GGNNCC 2 cut(s) 40, 1051
PspPI GGNCC 2 cut(s) 39, 1373
PstI CTGCAG 1 cut(s) 169
PsuI RGATCY 1 cut(s) 1025
RsaI GTAC 7 cut(s) 122, 267, 590, 630, 700, 1176, 1323
RsaNI GTAC 7 cut(s) 121, 266, 589, 629, 699, 1175, 1322
RseI CAYNNNNRTG 2 cut(s) 930, 1476
SaqAI TTAA 8 cut(s) 297, 557, 617, 711, 743, 879, 951, 1239
SatI GCNGC 2 cut(s) 435, 1061
Sau3AI GATC 8 cut(s) 91, 160, 277, 388, 481, 955, 1025, 1456
Sau96I GGNCC 2 cut(s) 39, 1373
SchI GAGTC 1 cut(s) 149
SduI GDGCHC 1 cut(s) 419
SfaNI GCATC 2 cut(s) 662, 886
SfcI CTRYAG 2 cut(s) 165, 1069
SinI GGWCC 2 cut(s) 39, 1373
SmiMI CAYNNNNRTG 2 cut(s) 930, 1476
SmlI CTYRAG 2 cut(s) 95, 857
SmoI CTYRAG 2 cut(s) 95, 857
SnaBI TACGTA 1 cut(s) 632
SsiI CCGC 3 cut(s) 262, 291, 1118
SspI AATATT 1 cut(s) 1146
SspMI CTAG 4 cut(s) 47, 542, 1163, 1197
TaaI ACNGT 1 cut(s) 741
TaiI ACGT 5 cut(s) 91, 271, 457, 634, 922
TaqI TCGA 4 cut(s) 85, 280, 375, 817
TatI WGTACW 1 cut(s) 1174
TfiI GAWTC 5 cut(s) 334, 754, 1259, 1339, 1483
Tru1I TTAA 8 cut(s) 297, 557, 617, 711, 743, 879, 951, 1239
Tru9I TTAA 8 cut(s) 297, 557, 617, 711, 743, 879, 951, 1239
TscAI CASTG 2 cut(s) 796, 1107
TseFI GTSAC 6 cut(s) 23, 469, 844, 961, 1202, 1302
TseI GCWGC 2 cut(s) 434, 1060
Tsp45I GTSAC 6 cut(s) 23, 469, 844, 961, 1202, 1302
TspDTI ATGAA 9 cut(s) 194, 704, 803, 821, 914, 983, 1453, 1460, 1466
TspRI CASTG 2 cut(s) 796, 1107
VpaK11BI GGWCC 2 cut(s) 39, 1373
VspI ATTAAT 1 cut(s) 557
XapI RAATTY 3 cut(s) 691, 810, 1430
XbaI TCTAGA 1 cut(s) 1162
XceI RCATGY 1 cut(s) 1445
XmiI GTMKAC 1 cut(s) 1076
XspI CTAG 4 cut(s) 47, 542, 1163, 1197
Zsp2I ATGCAT 2 cut(s) 6, 689
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.