Rorug01G0353300

Plant transposase (Ptta/En/Spm family)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0000136
Physical Location & Seq
Forward (+)
47022482 .. 47029133
6652 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0353300.1

Sequence Viewer

Length: 3912 bp
ATGCCGCTATCAGGAAATGAAGAGACTGGGGTTAAACCCGTTACCTGGAAATCTAGTGATTATATTGCAGGTGTTCCTATCAAGAAAAGGAGGTTCCCAATGATGAGACATCCTTCACCCCCACCTGAAGAACCATCTTTTCCCCCATCAAAAATGGATTCAGTACAGAAGGAACAATCTAGCCAATCTCAGGGGTCGACTATTTCACATGCTAGTGTTGCAACTAGTTCCGGAATTTCTGATGCAACCAAAGCTTCTGGGTCTGATGATAGAAGAGGAAGCTCTGATGTTAAAAATGTTAATATGGTCCAAGGGAATGATAATACCGTTAGAGTCAAAGTTGAACAGCCTAGCCTTAGAGATCATTTGGGTTCTTCAGATGACTTGCAGAGGAAAGGGAAGTTTGTGATGGCTGATAATCCTGCACCCCAGATGACTTTAGGGAGAAATGAGTTGAATTTGAGACCAACTGAAGCTCCTGTATCTAGTTTAAGTAAGGATATTATGCATAGCAAACACAGAGCGGAAATGAAATGTAAAGAAGAGATGCCTGCTATTGCTGAGGGTACTGAATTATCATTAGCTTTGAAGGAACGTCTTTTTCCATCTTTGACAGGCCAAAAGAGTGATAGTGGTGTGAGTCACCCTAGTCAGGATAAGTCAGAACCTATTTCCCTGAATTTGTCTTTAAGTAAAGAGAAAATCAGTGTTCAATGCAAAGGTAAGGACAGAGATTTGAAAGTCCATGGTGCTCAGTTGCAAGCATGTAGAGCAAACTGGGATTTGAATACACCAATGGATGCATGGGGAGATTCTGTTAATGATGCTCCAGTCTCTGTTGATCGGATCAGTGCAAAAGGTGGGGGACATGCTGTTAGCTCAATTGGGATGGTTGAAGGTGGTGTTAGCTCAGAAAGACAGAGTAATGTTGAGAGTCACAAAAGAACTAATTTGACCATGTTGTCCAAACTAGATAGCCAGCAATATAAGCCCAATGATTCTCTTCTTTTACGCCTTAGTTCTTCTTGCTCACTGCCAAACCTGTGTCAAACACCTAGTTCATCCATTGCATTTGAGTTGGATAGGAGGACTTCTGCTGTAAACTTGCCTAGGGTGGCAGGGCCAACTAGCAATCTGAACTTGGGTAATCATATAACCGTGAAACCAGAGCCATTTGATGAGAGAGTCAAACAGGAACCCAGTAGTATGGGACCATTAAATATCAGAGCAGGGAAGCATGGAGTAGTTGAGCGTTCCACTGTTGGAGCTGTTCACTCCTCAAATGTTAGCACCCAAAAATTTGTTGATCCTAGATCCATAAAATCTGAACCAGCTCTTGAGCTTAATCAAGAGATGAGCAAGTCTACCAAAGGGAAATCAGTCCAACTGGACAAACATGTGATAAATGGTCCAGATGATCGTCCTTTTGACATGAAATTGCCTGTTGCTGCAGAGATATTTCGCCCTGCTGGAAAGCCTTCTTGTTTGACAGAGTCGACTGGGCAGCCTTCTTGTTCAACAGAGTTGACTGTGACTCAGGATGTGATAAACCATTCAGGAGACTCTAATGGAAGGCTGCCTCATGAAGCTTGTCAAAGCAATGAGAAAGTTGCTATATCCCTGGGCCATGATAGTAAGGTAAACAACATGCGAACTAAAGATGACAATGTTGATAGTCAGAGTTGTAAATTGAAATTGATGATTGATCAACCCCTTGATTCTCGTGGAAGTGGTGGAACCAGTGATGAGGAAAAGATAAATATATCAACAGATATGCTGGAAGATTCTTATGGTACTGATTATGAATCAGATGGTAATCATGCTATAGATACAGCTATTGAGACTAAAGAAGGTGGTAAAGATGATGACTATGAAGATGGCGAGGTCAGAGATTTACTTCTGGATACAGCAGTCCAGGATGAGCTCATAAGTGAGTCAAGAGAAGTGGAAAATGCCAACCATGATGATTTTGATAATGGAAGGACAGAGTGTGTGGGACCTGTTGATATTGTTCATCATACTTCATTCCATGTTGAGGCAAAAGACAACAAAACAGACAATCTTGCTGGAACAAGTAACAATGATCATGAAGAATCTGCTGATATAGATCTTAATATTGAATCTGACAAGGGCTGTGATAATGATGTGTGCTTGCAAGTAGCATCGTTTGTTGAAAAGCTAACGTGCTCTGAGGGCATGAAAGGGTCCATCAGCACTGTGACAATAGAACCACTCAATGATCAATCTGGGAAGAGAGATTCCCAGAAGTGTCAGGATGCAGAGTTATCCTCGGAGCAAGTCACTATAGGAAGCCAAGGAACTGCAGTAGCTAGTGCTCAGGATACTGAGCTGAATGTTAATAAAACTGAACCTGTACTGACGAGTGATTCAACTTTGTCCAAAACTTCTGGTAGTGGTGATAATGCGACTAAGGATACCAGTAATGGAGGACAGCGGAGCCGGATTATAACTTTGCCTCGATCTTTAAATGGGTCTCCTACTAGATCAAGATCAATTCCAGACCAGCCATTTCCATCAAGAGCTGGAAGAGAAATTTTACCTGCTGTAACACCAGACGACAATAAACTACATCCTCGAGGGAGAGGTGAAACGTATGTTGACGATACCTACAGATTTCCAAGGGAGCGGTACCAAGATCAGTCATGGAGGAATTCAGTGAATTTTAGACGTGGTAGGGGGAGGATGAATAACCGTGGGGATTGGAGTTCTGATCGTTACTTTGCAGCGGAGCGTTATTATAATCAAACAAAGTACCGTGTTCCAAGACATAAATATGTAAATGTCGTTTCTGACGCTGACCTCGAATATAATAATTATAATATGGCTTCAGATGGTGCATTTGTTGGCCGTGGAGGGAGGAAGCTTTCAAATGATGGACCAATTAACCACCGTATACCCTCCAGGAGACGGTCTCCTGTTGGGGGACCTATAATTCATGTGCCTCGCAGAAATCCCAGAAATTTTAGCCCAAGTAGATGCATTGGTGAAGATACTTCAGATTTGGTTGGCATGAGGCATAATGAGAAGCTTATGAGAGGTTTTCCTGATGATTCTGTAGATCCCATGTTCAATCGTACCCAACCTTCATATGAGGGAGATGGTCAGTTTGGACGAGGGCGAGGAAATAGGACCTTTTCTTTTGTTCAGCAAAGAGGTATTCGAGTTCGTTCAAAGTCTCCAATAAGGACCAGAACTCGTTCTCCTGTGCCATGGTCATCTCCTAGAAGAAGATCCCCTGATGGATTTGGTGGGCCTGGAGAATTGAGCCATCGAACACCAAATTACAGGATGGAGAGATTTAGATCCCCTGATGGGGCATCTTTTACCGGAGAAATGGTGGTCAGAAGAAATCCGCCAAATGATTTGAGGGATATGGATACTGTAAGAGACCATGGTCATCAAAGGTCTGGCATTCATAGGAGTCCTTCTTCCCGTGTTTTACTTAGAAACAGGAGATTTGATGTCATAGATCCTCGAGAAAGGGCGGACAATAATGAGTTCTTTGCGGGTGGGGGGCCTATGCATTCTGGTCGGTTGCATGAGCTTGGTGGGGATGGAAATGCTGATGAGAGGAGAAGGTTTGGTGAGAGACGGGGACCTGTCCGTACATTTAGGCCTCCTTATAACAGTGCTGATGGTGAGACATTTCATGTTAACACAGAGGATGGCCCTAGGCCTTTCCGGTTCTGCCCAGATGGTGAAACTGAGTTTCAAGAAAGAGGCAATTTGAGAGAGAGAGATTTTGACAGAAGGATAAAGAACAGGCCAGGAAATGCACCTAGAAGAATGAGAAGCATTGAAGATCAGGAAGGAAATTTCAGGAGTGATGGACAGCCATGGAATGATGGTGGTTTTGATGATATCTCACGGGCGAAGAGAAAAAGGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Transposase_23 Transposase_24

Protein Analysis

1303

Amino Acids

144.33

Weight (kDa)

6.84

Isoelectric Point (pI)

55.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000136)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g28181 FvH4_1g28182 FvH4_1g28940 FvH4_1g29801 FvH4_1g29802 FvH4_3g03321 FvH4_3g12341 FvH4_3g22002 FvH4_3g22003 FvH4_3g22852 FvH4_3g22860 FvH4_3g32806 FvH4_4g02410 FvH4_4g08110 FvH4_4g09791 FvH4_4g09792 FvH4_4g09830 FvH4_4g09831 FvH4_4g09832 FvH4_4g23330 FvH4_5g25220 FvH4_5g30160 FvH4_5g32340 FvH4_5g37421 FvH4_6g21851 FvH4_6g21852 FvH4_6g22855 FvH4_6g22870 FvH4_6g30440 FvH4_6g30962 FvH4_6g37930
malus_domestica MD16G1158000.v1.1
prunus_persica Prupe.1G266000_v2.0.a1 Prupe.1G266100_v2.0.a1 Prupe.1G266200_v2.0.a1 Prupe.2G006600_v2.0.a1 Prupe.2G110300_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0322121 RchiOBHm_Chr1g0322131 RchiOBHm_Chr1g0341871 RchiOBHm_Chr1g0346721 RchiOBHm_Chr2g0118081 RchiOBHm_Chr2g0138651 RchiOBHm_Chr3g0468651 RchiOBHm_Chr3g0475981 RchiOBHm_Chr4g0416921 RchiOBHm_Chr5g0068021 RchiOBHm_Chr6g0273901 RchiOBHm_Chr6g0278661 RchiOBHm_Chr6g0281481 RchiOBHm_Chr6g0306081 RchiOBHm_Chr6g0306091 RchiOBHm_Chr7g0220731 RchiOBHm_Chr7g0220741 RchiOBHm_Chr7g0222641 RchiOBHm_Chr7g0225401 RchiOBHm_Chr7g0230481
rosa_laevigata RLG00000001569 RLG00000006184 RLG00000007906 RLG00000009863 RLG00000011359 RLG00000016125 RLG00000018543 RLG00000020693 RLG00000029658 RLG00000029716
rosa_multiflora Rmu_co8244591.1_g000001 Rmu_co8396391.1_g000001 Rmu_sc0000120.1_g000013 Rmu_sc0000239.1_g000047 Rmu_sc0000252.1_g000019 Rmu_sc0000322.1_g000037 Rmu_sc0000376.1_g000010 Rmu_sc0000435.1_g000033 Rmu_sc0001872.1_g000001 Rmu_sc0001992.1_g000011 Rmu_sc0001992.1_g000012 Rmu_sc0002231.1_g000010 Rmu_sc0002372.1_g000033 Rmu_sc0002705.1_g000012 Rmu_sc0003435.1_g000015 Rmu_sc0004084.1_g000042 Rmu_sc0004084.1_g000043 Rmu_sc0004755.1_g000011 Rmu_sc0005391.1_g000010 Rmu_sc0005970.1_g000001 Rmu_sc0006163.1_g000003 Rmu_sc0006422.1_g000004 Rmu_sc0006422.1_g000005 Rmu_sc0006952.1_g000002 Rmu_sc0010937.1_g000007 Rmu_sc0012130.1_g000008 Rmu_sc0024120.1_g000001 Rmu_sc0035618.1_g000001
rosa_roxburghii Rroxscaffold_1G00039230 Rroxscaffold_2G00085900 Rroxscaffold_2G00085910 Rroxscaffold_2G00125240 Rroxscaffold_2G00126850 Rroxscaffold_2G00150980 Rroxscaffold_3G00230580 Rroxscaffold_3G00245050 Rroxscaffold_4G00320680 Rroxscaffold_4G00328800 Rroxscaffold_5G00349640 Rroxscaffold_6G00406600 Rroxscaffold_7G00165570 Rroxscaffold_7G00191490 Rroxscaffold_7G00192700 Rroxscaffold_7G00192710 Rroxscaffold_7G00201190 Rroxscaffold_7G00201620
rosa_rugosa Rorug01G0033100 Rorug01G0089100 Rorug01G0102000 Rorug01G0353100 Rorug01G0353200 Rorug01G0353300 Rorug02G0276900 Rorug02G0362200 Rorug02G0445100 Rorug02G0627600 Rorug02G0627600 Rorug02G0627600 Rorug03G0267700 Rorug03G0267800 Rorug03G0327400 Rorug04G0004500 Rorug04G0006700 Rorug04G0061500 Rorug04G0064700 Rorug04G0073300 Rorug04G0094300 Rorug04G0181200 Rorug04G0181200 Rorug04G0330100 Rorug05G0035600 Rorug05G0038600 Rorug05G0201600 Rorug05G0367700 Rorug05G0407600 Rorug05G0407700 Rorug05G0543400 Rorug06G0012200 Rorug06G0363700.1 Rorug07G0085100
rosa_samantha Rh1AG049700 Rh1AG182700 Rh1AG182800 Rh1BG015500 Rh1BG398700 Rh1BG409100 Rh1CG074100 Rh1CG169200 Rh1CG169300 Rh1CG169400 Rh1DG130800 Rh1DG130900 Rh1DG440400 Rh2DG440900 Rh3AG144700 Rh3BG167100 Rh3DG366800 Rh4AG214400 Rh4BG189700 Rh4BG189800 Rh4CG268600 Rh4DG004400 Rh4DG147900 Rh4DG248300 Rh5AG053900 Rh5AG054000 Rh5AG317200 Rh5AG326400 Rh5AG332600 Rh5AG419700 Rh5CG284900 Rh5CG288600 Rh6AG252700 Rh6AG252800 Rh6AG256900 Rh6AG257000 Rh6AG257500 Rh6BG175300 Rh6DG226200 Rh6DG264500 Rh7AG374000 Rh7AG398100 Rh7BG274900 Rh7BG300500 Rh7BG308200 Rh7BG451200
rosa_wichuraiana Rw1G015860 Rw1G022160 Rw3G016160 Rw3G021170 Rw4G006020 Rw4G008740 Rw5G027400 Rw5G031840 Rw5G036940 Rw6G008320 Rw6G030330 Rw7G033450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 2468, 2760, 2838, 3645
AarI CACCTGC 1 cut(s) 59
AasI GACNNNNNNGTC 1 cut(s) 961
AbsI CCTCGAGG 1 cut(s) 2595
Acc36I ACCTGC 2 cut(s) 59, 2569
Acc65I GGTACC 1 cut(s) 2649
AccB1I GGYRCC 1 cut(s) 2649
AccBSI CCGCTC 2 cut(s) 524, 2647
AccI GTMKAC 4 cut(s) 197, 1364, 1496, 2914
AccIII TCCGGA 1 cut(s) 230
AciI CCGC 8 cut(s) 5, 524, 2455, 2647, 2747, 3374, 3506, 3527
AclWI GGATC 7 cut(s) 854, 1301, 1308, 3074, 3246, 3318, 3485
AcoI YGGCCR 1 cut(s) 2866
AcsI RAATTY 9 cut(s) 234, 457, 679, 1298, 2553, 2671, 2680, 2980, 3835
AcuI CTGAAG 5 cut(s) 147, 360, 492, 2832, 3000
AfaI GTAC 8 cut(s) 165, 568, 1795, 2376, 2651, 2774, 3097, 3628
AfiI CCNNNNNNNGG 9 cut(s) 11, 45, 190, 652, 2035, 2928, 2942, 3333, 3334
AflIII ACRYGT 1 cut(s) 1396
AhlI ACTAGT 1 cut(s) 224
AjiI CACGTC 1 cut(s) 2690
AjnI CCWGG 6 cut(s) 44, 1620, 1914, 2921, 3274, 3787
AloI GAACNNNNNNTCC 2 cut(s) 3205, 3237
Alw21I GWGCWC 4 cut(s) 754, 1926, 2189, 2338
AlwI GGATC 7 cut(s) 854, 1301, 1308, 3074, 3246, 3318, 3485
AlwNI CAGNNNCTG 1 cut(s) 836
Ama87I CYCGRG 2 cut(s) 2595, 3495
Aor13HI TCCGGA 1 cut(s) 230
ApeKI GCWGC 4 cut(s) 1448, 1504, 1576, 2744
ApoI RAATTY 9 cut(s) 234, 457, 679, 1298, 2553, 2671, 2680, 2980, 3835
Asp700I GAANNNNTTC 2 cut(s) 1477, 3217
Asp718I GGTACC 1 cut(s) 2649
AspA2I CCTAGG 2 cut(s) 1109, 3692
AsuHPI GGTGA 8 cut(s) 108, 635, 2429, 2618, 3017, 3617, 3671, 3731
AvaI CYCGRG 2 cut(s) 2595, 3495
AvrII CCTAGG 2 cut(s) 1109, 3692
BaeI ACNNNNGTAYC 2 cut(s) 1896, 1929
BanI GGYRCC 1 cut(s) 2649
BanII GRGCYC 1 cut(s) 1926
BarI GAAGNNNNNNTAC 2 cut(s) 3088, 3120
BauI CACGAG 1 cut(s) 1722
Bbv12I GWGCWC 4 cut(s) 754, 1926, 2189, 2338
BbvCI CCTCAGC 1 cut(s) 561
BbvI GCAGC 4 cut(s) 1435, 1516, 1563, 2756
BceAI ACGGC 1 cut(s) 2853
BcgI CGANNNNNNTGC 2 cut(s) 3533, 3567
BciT130I CCWGG 6 cut(s) 46, 1622, 1916, 2923, 3276, 3789
BciVI GTATCC 4 cut(s) 1897, 2335, 2428, 3391
BclI TGATCA 3 cut(s) 1705, 2083, 2239
BcuI ACTAGT 1 cut(s) 224
BfmI CTRYAG 6 cut(s) 1449, 1824, 2304, 2322, 2629, 3075
BfuAI ACCTGC 2 cut(s) 59, 2569
BfuI GTATCC 4 cut(s) 1897, 2335, 2428, 3391
BglII AGATCT 1 cut(s) 2107
BisI GCNGC 5 cut(s) 5, 1449, 1505, 1577, 2745
BlnI CCTAGG 2 cut(s) 1109, 3692
BlsI GCNGC 5 cut(s) 6, 1450, 1506, 1578, 2746
Bme1390I CCNGG 6 cut(s) 46, 1622, 1916, 2923, 3276, 3789
BmeT110I CYCGRG 2 cut(s) 2595, 3495
BmgBI CACGTC 1 cut(s) 2690
BmrFI CCNGG 6 cut(s) 46, 1622, 1916, 2923, 3276, 3789
BmrI ACTGGG 4 cut(s) 36, 787, 1194, 1509
BmsI GCATC 8 cut(s) 232, 537, 790, 814, 2171, 2266, 2987, 3347
BmuI ACTGGG 4 cut(s) 36, 787, 1194, 1509
BoxI GACNNNNGTC 1 cut(s) 3414
BplI GAGNNNNNCTC 4 cut(s) 2273, 2305, 2917, 2949
BpmI CTGGAG 3 cut(s) 813, 2905, 3297
Bpu10I CCTNAGC 2 cut(s) 561, 2337
BpuEI CTTGAG 1 cut(s) 1358
BsaBI GATNNNNATC 4 cut(s) 1815, 2106, 2509, 3322
BsaI GGTCTC 4 cut(s) 457, 2499, 2937, 3402
BsaWI WCCGGW 3 cut(s) 230, 3347, 3702
BsaXI ACNNNNNCTCC 8 cut(s) 460, 490, 801, 831, 3205, 3235, 3342, 3372
Bsc4I CCNNNNNNNGG 9 cut(s) 11, 45, 190, 652, 2035, 2928, 2942, 3333, 3334
Bse1I ACTGG 8 cut(s) 31, 782, 830, 1200, 1392, 1504, 1740, 2439
Bse3DI GCAATG 2 cut(s) 1065, 1606
Bse8I GATNNNNATC 4 cut(s) 1815, 2106, 2509, 3322
BseAI TCCGGA 1 cut(s) 230
BseBI CCWGG 6 cut(s) 46, 1622, 1916, 2923, 3276, 3789
BseJI GATNNNNATC 4 cut(s) 1815, 2106, 2509, 3322
BseLI CCNNNNNNNGG 9 cut(s) 11, 45, 190, 652, 2035, 2928, 2942, 3333, 3334
BseMI GCAATG 2 cut(s) 1065, 1606
BseMII CTCAG 9 cut(s) 203, 552, 767, 924, 1550, 2181, 2337, 2351, 3717
BseNI ACTGG 8 cut(s) 31, 782, 830, 1200, 1392, 1504, 1740, 2439
BseRI GAGGAG 2 cut(s) 1267, 3607
BseXI GCAGC 4 cut(s) 1435, 1516, 1563, 2756
BsgI GTGCAG 1 cut(s) 408
BshNI GGYRCC 1 cut(s) 2649
BsiHKAI GWGCWC 4 cut(s) 754, 1926, 2189, 2338
BsiHKCI CYCGRG 2 cut(s) 2595, 3495
BsiSI CCGG 4 cut(s) 231, 2461, 3348, 3703
BslFI GGGAC 5 cut(s) 879, 1224, 2010, 2958, 3630
BslI CCNNNNNNNGG 9 cut(s) 11, 45, 190, 652, 2035, 2928, 2942, 3333, 3334
BsmBI CGTCTC 2 cut(s) 2920, 3604
BsmFI GGGAC 5 cut(s) 879, 1224, 2010, 2958, 3630
BsmI GAATGC 2 cut(s) 3432, 3544
Bso31I GGTCTC 4 cut(s) 457, 2499, 2937, 3402
BsoBI CYCGRG 2 cut(s) 2595, 3495
Bsp1286I GDGCHC 4 cut(s) 754, 1926, 2189, 2338
Bsp13I TCCGGA 1 cut(s) 230
Bsp19I CCATGG 4 cut(s) 745, 3230, 3412, 3857
BspACI CCGC 8 cut(s) 5, 524, 2455, 2647, 2747, 3374, 3506, 3527
BspCNI CTCAG 9 cut(s) 202, 553, 766, 923, 1549, 2182, 2338, 2350, 3718
BspEI TCCGGA 1 cut(s) 230
BspHI TCATGA 2 cut(s) 1582, 2086
BspMAI CTGCAG 2 cut(s) 1453, 2326
BspMI ACCTGC 2 cut(s) 59, 2569
BspPI GGATC 7 cut(s) 854, 1301, 1308, 3074, 3246, 3318, 3485
BspT107I GGYRCC 1 cut(s) 2649
BspTNI GGTCTC 4 cut(s) 457, 2499, 2937, 3402
BsrBI CCGCTC 2 cut(s) 524, 2647
BsrDI GCAATG 2 cut(s) 1065, 1606
BsrI ACTGG 8 cut(s) 31, 782, 830, 1200, 1392, 1504, 1740, 2439
BssNAI GTATAC 1 cut(s) 2915
BssSI CACGAG 1 cut(s) 1722
BssT1I CCWWGG 9 cut(s) 310, 745, 1109, 2314, 2639, 3230, 3412, 3692, 3857
Bst1107I GTATAC 1 cut(s) 2915
Bst2BI CACGAG 1 cut(s) 1722
Bst2UI CCWGG 6 cut(s) 46, 1622, 1916, 2923, 3276, 3789
Bst6I CTCTTC 7 cut(s) 15, 268, 537, 1008, 2246, 2542, 3890
BstC8I GCNNGC 4 cut(s) 552, 762, 980, 2153
BstDSI CCRYGG 6 cut(s) 745, 2713, 2869, 3230, 3412, 3857
BstMWI GCNNNNNNNGC 5 cut(s) 218, 251, 770, 988, 2193
BstNI CCWGG 6 cut(s) 46, 1622, 1916, 2923, 3276, 3789
BstNSI RCATGY 5 cut(s) 212, 768, 872, 1400, 1651
BstPAI GACNNNNGTC 1 cut(s) 3414
BstSCI CCNGG 6 cut(s) 44, 1620, 1914, 2921, 3274, 3787
BstSFI CTRYAG 6 cut(s) 1449, 1824, 2304, 2322, 2629, 3075
BstV1I GCAGC 4 cut(s) 1435, 1516, 1563, 2756
BstX2I RGATCY 6 cut(s) 1313, 2107, 3079, 3251, 3323, 3490
BstXI CCANNNNNNTGG 1 cut(s) 1207
BstYI RGATCY 6 cut(s) 1313, 2107, 3079, 3251, 3323, 3490
BstZ17I GTATAC 1 cut(s) 2915
BsuI GTATCC 4 cut(s) 1897, 2335, 2428, 3391
BtgI CCRYGG 6 cut(s) 745, 2713, 2869, 3230, 3412, 3857
BtrI CACGTC 1 cut(s) 2690
BtsI GCAGTG 1 cut(s) 1031
BtsIMutI CAGTG 8 cut(s) 712, 856, 1031, 1257, 1747, 2214, 2682, 3655
BveI ACCTGC 2 cut(s) 59, 2569
Cac8I GCNNGC 4 cut(s) 552, 762, 980, 2153
CaiI CAGNNNCTG 1 cut(s) 836
CciI TCATGA 2 cut(s) 1582, 2086
CseI GACGC 1 cut(s) 2822
Csp6I GTAC 8 cut(s) 164, 567, 1794, 2375, 2650, 2773, 3096, 3627
CspCI CAANNNNNGTGG 2 cut(s) 1926, 1961
CviQI GTAC 8 cut(s) 164, 567, 1794, 2375, 2650, 2773, 3096, 3627
DraI TTTAAA 1 cut(s) 2487
DrdI GACNNNNNNGTC 1 cut(s) 961
DseDI GACNNNNNNGTC 1 cut(s) 961
EaeI YGGCCR 1 cut(s) 2866
Eam1104I CTCTTC 7 cut(s) 15, 268, 537, 1008, 2246, 2542, 3890
EarI CTCTTC 7 cut(s) 15, 268, 537, 1008, 2246, 2542, 3890
EciI GGCGGA 2 cut(s) 3363, 3521
Ecl136II GAGCTC 1 cut(s) 1924
Eco130I CCWWGG 9 cut(s) 310, 745, 1109, 2314, 2639, 3230, 3412, 3692, 3857
Eco147I AGGCCT 2 cut(s) 3637, 3697
Eco24I GRGCYC 1 cut(s) 1926
Eco31I GGTCTC 4 cut(s) 457, 2499, 2937, 3402
Eco32I GATATC 1 cut(s) 3883
Eco53kI GAGCTC 1 cut(s) 1924
Eco57I CTGAAG 5 cut(s) 147, 360, 492, 2832, 3000
Eco88I CYCGRG 2 cut(s) 2595, 3495
EcoICRI GAGCTC 1 cut(s) 1924
EcoO109I RGGNCCY 5 cut(s) 1997, 2945, 3150, 3536, 3617
EcoRI GAATTC 1 cut(s) 2671
EcoRII CCWGG 6 cut(s) 44, 1620, 1914, 2921, 3274, 3787
EcoRV GATATC 1 cut(s) 3883
EcoT14I CCWWGG 9 cut(s) 310, 745, 1109, 2314, 2639, 3230, 3412, 3692, 3857
EcoT22I ATGCAT 4 cut(s) 510, 805, 3002, 3546
EcoT38I GRGCYC 1 cut(s) 1926
ErhI CCWWGG 9 cut(s) 310, 745, 1109, 2314, 2639, 3230, 3412, 3692, 3857
Esp3I CGTCTC 2 cut(s) 2920, 3604
FaqI GGGAC 5 cut(s) 879, 1224, 2010, 2958, 3630
FauI CCCGC 1 cut(s) 3520
FauNDI CATATG 1 cut(s) 3109
FbaI TGATCA 3 cut(s) 1705, 2083, 2239
FblI GTMKAC 4 cut(s) 197, 1364, 1496, 2914
Fnu4HI GCNGC 5 cut(s) 5, 1449, 1505, 1577, 2745
FriOI GRGCYC 1 cut(s) 1926
Fsp4HI GCNGC 5 cut(s) 5, 1449, 1505, 1577, 2745
GluI GCNGC 5 cut(s) 5, 1449, 1505, 1577, 2745
GsuI CTGGAG 3 cut(s) 813, 2905, 3297
HapII CCGG 4 cut(s) 231, 2461, 3348, 3703
HgaI GACGC 1 cut(s) 2822
HincII GTYRAC 5 cut(s) 198, 1497, 1527, 2620, 3676
HindII GTYRAC 5 cut(s) 198, 1497, 1527, 2620, 3676
HindIII AAGCTT 4 cut(s) 252, 1587, 2882, 3047
HpaI GTTAAC 1 cut(s) 3676
HpaII CCGG 4 cut(s) 231, 2461, 3348, 3703
HphI GGTGA 8 cut(s) 108, 635, 2429, 2618, 3017, 3617, 3671, 3731
HpyCH4IV ACGT 4 cut(s) 595, 2183, 2612, 2689
HpyF10VI GCNNNNNNNGC 5 cut(s) 218, 251, 770, 988, 2193
HpySE526I ACGT 4 cut(s) 595, 2183, 2612, 2689
Kpn2I TCCGGA 1 cut(s) 230
KpnI GGTACC 1 cut(s) 2653
Ksp22I TGATCA 3 cut(s) 1705, 2083, 2239
KspAI GTTAAC 1 cut(s) 3676
LmnI GCTCC 7 cut(s) 481, 832, 1265, 2293, 2457, 2644, 2749
Lsp1109I GCAGC 4 cut(s) 1435, 1516, 1563, 2756
LweI GCATC 8 cut(s) 232, 537, 790, 814, 2171, 2266, 2987, 3347
MaeII ACGT 4 cut(s) 595, 2183, 2612, 2689
MaeIII GTNAC 9 cut(s) 40, 641, 935, 1531, 2075, 2218, 2299, 2566, 2735
MbiI CCGCTC 2 cut(s) 524, 2647
MfeI CAATTG 1 cut(s) 882
MflI RGATCY 6 cut(s) 1313, 2107, 3079, 3251, 3323, 3490
MhlI GDGCHC 4 cut(s) 754, 1926, 2189, 2338
MlyI GAGTC 9 cut(s) 342, 649, 943, 1194, 1502, 1528, 1556, 1943, 3451
MmeI TCCRAC 3 cut(s) 1059, 1243, 1408
Mph1103I ATGCAT 4 cut(s) 510, 805, 3002, 3546
MroI TCCGGA 1 cut(s) 230
MroXI GAANNNNTTC 2 cut(s) 1477, 3217
MslI CAYNNNNRTG 2 cut(s) 213, 2793
MspA1I CMGCKG 2 cut(s) 2455, 2747
MspI CCGG 4 cut(s) 231, 2461, 3348, 3703
MspR9I CCNGG 6 cut(s) 46, 1622, 1916, 2923, 3276, 3789
MunI CAATTG 1 cut(s) 882
Mva1269I GAATGC 2 cut(s) 3432, 3544
MvaI CCWGG 6 cut(s) 46, 1622, 1916, 2923, 3276, 3789
MwoI GCNNNNNNNGC 5 cut(s) 218, 251, 770, 988, 2193
NcoI CCATGG 4 cut(s) 745, 3230, 3412, 3857
NdeI CATATG 1 cut(s) 3109
NmuCI GTSAC 5 cut(s) 641, 935, 1531, 2218, 2299
NsiI ATGCAT 4 cut(s) 510, 805, 3002, 3546
NspI RCATGY 5 cut(s) 212, 768, 872, 1400, 1651
PaeR7I CTCGAG 2 cut(s) 2595, 3495
PagI TCATGA 2 cut(s) 1582, 2086
PaqCI CACCTGC 1 cut(s) 59
PasI CCCWGGG 1 cut(s) 1621
PceI AGGCCT 2 cut(s) 3637, 3697
PciI ACATGT 1 cut(s) 1396
PcsI WCGNNNNNNNCGW 1 cut(s) 2820
PctI GAATGC 2 cut(s) 3432, 3544
PdmI GAANNNNTTC 2 cut(s) 1477, 3217
PflFI GACNNNGTC 1 cut(s) 1492
PfoI TCCNGGA 2 cut(s) 1914, 2921
PkrI GCNGC 5 cut(s) 6, 1450, 1506, 1578, 2746
PleI GAGTC 9 cut(s) 341, 648, 942, 1193, 1501, 1528, 1556, 1942, 3450
PpsI GAGTC 9 cut(s) 341, 648, 942, 1193, 1501, 1528, 1556, 1942, 3450
PpuMI RGGWCCY 4 cut(s) 1997, 2945, 3150, 3617
PscI ACATGT 1 cut(s) 1396
PshAI GACNNNNGTC 1 cut(s) 3414
PsiI TTATAA 4 cut(s) 2468, 2760, 2838, 3645
Psp124BI GAGCTC 1 cut(s) 1926
Psp5II RGGWCCY 4 cut(s) 1997, 2945, 3150, 3617
Psp6I CCWGG 6 cut(s) 44, 1620, 1914, 2921, 3274, 3787
PspGI CCWGG 6 cut(s) 44, 1620, 1914, 2921, 3274, 3787
PspPPI RGGWCCY 4 cut(s) 1997, 2945, 3150, 3617
PspXI VCTCGAGB 1 cut(s) 2595
PstI CTGCAG 2 cut(s) 1453, 2326
PstNI CAGNNNCTG 1 cut(s) 836
PsuI RGATCY 6 cut(s) 1313, 2107, 3079, 3251, 3323, 3490
PsyI GACNNNGTC 1 cut(s) 1492
RsaI GTAC 8 cut(s) 165, 568, 1795, 2376, 2651, 2774, 3097, 3628
RsaNI GTAC 8 cut(s) 164, 567, 1794, 2375, 2650, 2773, 3096, 3627
RseI CAYNNNNRTG 2 cut(s) 213, 2793
SacI GAGCTC 1 cut(s) 1926
SalI GTCGAC 2 cut(s) 196, 1495
SatI GCNGC 5 cut(s) 5, 1449, 1505, 1577, 2745
SchI GAGTC 9 cut(s) 342, 649, 943, 1194, 1502, 1528, 1556, 1943, 3451
ScrFI CCNGG 6 cut(s) 46, 1622, 1916, 2923, 3276, 3789
SduI GDGCHC 4 cut(s) 754, 1926, 2189, 2338
SfaNI GCATC 8 cut(s) 232, 537, 790, 814, 2171, 2266, 2987, 3347
SfcI CTRYAG 6 cut(s) 1449, 1824, 2304, 2322, 2629, 3075
Sfr274I CTCGAG 2 cut(s) 2595, 3495
SlaI CTCGAG 2 cut(s) 2595, 3495
SmiMI CAYNNNNRTG 2 cut(s) 213, 2793
SmlI CTYRAG 3 cut(s) 1337, 2595, 3495
SmoI CTYRAG 3 cut(s) 1337, 2595, 3495
SpeI ACTAGT 1 cut(s) 224
SseBI AGGCCT 2 cut(s) 3637, 3697
SsiI CCGC 8 cut(s) 5, 524, 2455, 2647, 2747, 3374, 3506, 3527
SspI AATATT 1 cut(s) 2116
SstI GAGCTC 1 cut(s) 1926
StuI AGGCCT 2 cut(s) 3637, 3697
StyD4I CCNGG 6 cut(s) 44, 1620, 1914, 2921, 3274, 3787
StyI CCWWGG 9 cut(s) 310, 745, 1109, 2314, 2639, 3230, 3412, 3692, 3857
TaiI ACGT 4 cut(s) 598, 2186, 2615, 2692
TaqI TCGA 8 cut(s) 197, 1496, 2479, 2596, 2823, 3181, 3292, 3496
TatI WGTACW 2 cut(s) 163, 2374
TauI GCSGC 1 cut(s) 7
TscAI CASTG 8 cut(s) 712, 856, 1038, 1264, 1747, 2221, 2682, 3655
TseFI GTSAC 5 cut(s) 641, 935, 1531, 2218, 2299
TseI GCWGC 4 cut(s) 1448, 1504, 1576, 2744
Tsp45I GTSAC 5 cut(s) 641, 935, 1531, 2218, 2299
TspGWI ACGGA 1 cut(s) 3614
TspRI CASTG 8 cut(s) 712, 856, 1038, 1264, 1747, 2221, 2682, 3655
Tth111I GACNNNGTC 1 cut(s) 1492
XapI RAATTY 9 cut(s) 234, 457, 679, 1298, 2553, 2671, 2680, 2980, 3835
XceI RCATGY 5 cut(s) 212, 768, 872, 1400, 1651
XcmI CCANNNNNNNNNTGG 1 cut(s) 801
XhoI CTCGAG 2 cut(s) 2595, 3495
XmaJI CCTAGG 2 cut(s) 1109, 3692
XmiI GTMKAC 4 cut(s) 197, 1364, 1496, 2914
XmnI GAANNNNTTC 2 cut(s) 1477, 3217
Zsp2I ATGCAT 4 cut(s) 510, 805, 3002, 3546
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.