Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb1

Orthogroup ID

OG0000310

Physical Location & Seq

Genomic Coordinates Reverse (-)

14705139 .. 14716092

Gene Structure 10954 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_1g22730.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4599 bp

ATGGCGGACGACGAGCAACCGATCGGCGGGAGGCGACGGAGCCGAGGAGCCGATGCGGCGGCGCGGTCCAGCGCCCTGGAGCGTCTCAAAGCTCTCCGTGAAGGCGGCCGCCGCTCGGGAAACTCCGACGACCGGTTCCAGATCAAGCTGGAGAAGCCGATCTACGACACGGTAGACGAGGACGAGTACGACGCGATTAAAGCCAAGCGCCGCGAGGAGTTCGGGGACTTCATCGAGGACGACGACGGCCTAGGGTACCGCGACGACGGCGAGGAAGAGGACTGGACACGCCGCGGGCTGCCTACGTCGTCGGATGAATCGGACGGCGATGCGAGGCCGAAGAGGAAGAAGGTGGAGAAGAAGGAGAAGGAGAAGGAGCCTCGGGTGAAAAAGCCCAACGCGTCGCTCACGGCGGCGGCGGCGATGATGGGGAAGCAGCGGCTTTCGTCGATGTTCACGTCGTCGGTGTTTAATAAGAGTAGGGATGGTGACAAGGGTAAGGGATTGTCTTGTGATAGTATTGTGGATGAGGTGATTGCGGAGTTTGCGCCGGATGAGGCTGATAGGGAGAAGCGGCGGAGGGCGCAGCCGGTGAAGAGTTTCGTTCCAATTAGGGGTGTCAAGAGTGAGAGAGTGAGTAATGGGGGTGTTGATTTAGCAAGGAGGCTTGAATTGGATCGAATTGTTGCGAATGGCGGTGTTTCTGTGGATAGTAAGGAAATGGATAAAGATTCGGAGGTGGATTTCGGAAATTGTAAGCCACAAATTGAGCAATTGCACGATTTTTTGGAGAATAGCGAGGTGCCTGGTGAGAAGAAGACTGAGGATTCTGTTGAGGTGTTGGCGGAGCCTGTGGTGAAGAAGGAAGACGTTTTCACTTTGAATGCAAAGATTAAGGAAGCTGATGATCCGGCATTGAGTGCTATGGCCGGATGGAAGGCGGCGATGACTGGTGGTGGTGGTGCTGGGGTTACTGGGGCAGACAGTGTGGAGGTCAAAGCTAGTGAAGGGAGTTTGGATTTTGATCTGGAATCAGATGGGTCACTGCCCTTTTACATGCTTGATGCTTACGAAGAGTTCTATGGACCTAGTATGGGCATTGTTTATCTATTTGGGAAGGTTAACGTAGGAAGTTCATATCAGAGTTGTTGTGCGGTTGTCAAGAACATGCAAAGATGTGTCTATGCAATTCCTGACAGTCCTTTAATTCAAACTGATGAAATGATGAAGCTCGAAAAGGATGTTGAAGAGTCAAGGATTTCACCCACAGATTTTCGAAAGAAGCTGCATGACGCAGCTTCAGAATTAAAGAACGAAATAGCAAAGAAGTTGTTGGATCTCAATGTATCAGCTTTTAGCATGACACCTGTCAAGAGGAACTATGCTTTTGAGCGAACTGACATACCAGTAGGGGAAAATCATGTACTGAAGATAAATTATCCATTCCGGGATCCCCCACTATCAGCAGATCTAAAAGGAGAAACTTTTTCTGCTCTTCTGGGAACTCATAGCAGCGCATTGGAACTTTTCCTTGTTAAAAGGAAGATAAAAGGACCTTCTTGGCTATCAGTTTCAAATTTTTCTGTCTGCCCAGCTCCACAACGGGTAAGCTGGTGCAAATTTGAGGTGATCGTCAATGCTCCGAAAGATATAAGGGTCTCAGCTTCCACGAAAAAAACTGTAGATATCCCTCTATTGGTTGTTACTGCAATAAGTTTGAAAACTATAATCAATGAGAAGCAGAATGTTAACGAAATTGTCTCTGCATCTGTTGTCTGCTGTAACAAGGCCAAGATTGACACTCCCGTGAAGGACTCAGAATGGAAGAGTCCTGGTATGCTGAGCCACTTTACTGTTGTCCGTAAGCTTGATGGAGGCATATTTCCCATGGGGTTTACTAAACTAGCTGGAGATAAGAATTCTAAAGCTGGATCAAATGTCTTGAGCATTGAGGGCAGTGAAAGGGCTTTGCTGAACCGTTTGTTCATTGAATTATACAAGTTGGATAGTGATGTTCTCGTTGGACATAATATATCAGGATTTGATTTGGACGTTCTTCTCCACAGAGCTCAGGTTTGCAGAGTGCCAAGCAGCATGTGGTCCAAAATAGGCCGCCTCAAGCGTTCTGTGATGCCTAAGCTTGCAAAAGGAAGCACCATTTTTGGCTCTGGAGCAAGTCCAGGGATCATGTCCTGCATTGCTGGTAGGCTGTTGTGTGATACATACTTGTGTTCCCGTGACTTATTAAAGGAGGTTAGCTATTCTTTGACACAACTTGCACAGACTCAGCTGGGCCAAAACCGGAAGGAGATCACACCACATGATATACCTAGAATGTTCCAAAAATCTGAGTTTCTCATGGAACTTATTGAATATGGCGAGACAGATGCATGGTTGTCTATGGGACTCATGTTTCATTTGAGTGTTCTTCCTCTCACACGTCAGCTGACCAATATTAGTGGTAATCTGTGGGGGAAAACTCTCCAAGGTGCAAGGGCACAAAGAGTCGAATACCTCTTACTTCATGCTTTTCATGCGAAAAAGTATATAGTTCCGGACAAGATTCCTCATATGAAGGATACCAAATTGACAAAACGGAGAACAAATAAGGGAGTTGAGGAAAAAGCTGCTGAGGAGTTGGATGTAGGTGATGTCAATGTTGTTAATGATCCACAGAGTGGTCATGGGAAAGGCAAGAAAGGTCCTGCCTATGCTGGTGGATTGGTTTTGGAGCCAAAAAGAGGTTTATATGACAAGTATATATTACTTCTGGACTTCAATAGTCTTTACCCGTCTATTATTCAGGAATATAATATATGCTTCACGACTGTTGAAAGAAATTCAGAAGAATTGGTTTCTCATTTACCTTCTAGTCAGAGACCTGGACTTTTACCTGAGTTGCTAAAAAATCTAGTCGAAAGGAGGAGAAAGGTAAAGTCATGGATGAAGAATGCATCTGGTCTCAAGTTCCAGCAACTTGACATTCAACAGCAAGCCCTGAAGCTGACTGCAAACAGTATGTATGGATGCTTGGGATTTTCAAATTCAAGATTTTATGCAAAGCCACTAGCAGAGCTTATAACACTAAGGGGCAGGGAGATCCTACAAAGTACTGTGGATCTTGTTCAGAACAATTTGAACTTAGAGGTTATTTATGGTGATACCGATTCAATAATGATTTATAGTGGGCTGGATGATTGTGCAAAAGCAAAAGCAATTGCTGTAAAAGTAATTCAAGAGGTTAACAAGAAGTATAGATGTTTAGAAATTGATCTTGACGGCTTGTACAAGAGAATGCTGCTTCTCAAGAAAAAGAAATATGCAGCTGTGAAAGAGATAACGAAAAACGAGAAAAAATATGAGGTTATTGAGTGTAAGGGCCTTGATATGGTACGTCGTGACTGGAGCTTACTTTCAAAGGAAGTGGGAGATTTCTGCCTAAGTCAAATATTGTCAGGAGGGTCATCTGAGGATGTTGTTGAATCAATTCATAACTCTCTTACAAAGGTGCAAGAGGACATGAGAAAAGGACAAGTTCCCCTTGAGAAATACATCATCACCAAGACATTAACTAAACCACCAGAAGCCTACCCAGATGGCAAAAACCAGCCACATGTTCAAGTGGCGCAAAGATTGAGGCAAAATGGTTACTCTACTGGTTGCTCTGTTGGTGATACAGTTCCTTATATTATATGCTGTGAGCAGGGCATGAATTCAGATAATTCTACCGGGATTGCTCAACGTGCTAGACATCCTGATGAACTAAAACAAGAAGATGGAAAATGGATGATTGACATTGATTACTACTTGTCCCAACAGATTCATCCCGTTGTATCACGACTTTGTGCTTCAATTCAGGGTACCAGTCCCCAACGCTTGGCTGATTGTTTAGGGATTGATTCATCCAAGTTCAAAACTAATTCAAGTGAAGCTGTCAGAAATGATTCTTCCAGCTTGTTCTTTTCTACTGATGATGAAGACAGATATCGGGATTGCAAGTCTTTGATCTTAGCATGTCCTAGCTGCTCTGGTACTTTTGATTGTCCAGCCATCTTCAACTCCATTGCAAAGTCAATCAGTGAAAAGCCAACAAGGCCACAAGGCGAGGAATCTTCCAGTGATTTTTGGTGCAGATTGCGCTGTCCAAAATGCCCAGAGGAAGGTGATGTGGGCAGACTGTCTCCTGTAATGATAGCAAATCAGGTCAAAAGGCAAGCAGACAGTTTTGTTTCAATGTACTACAAGGGCATAATGACGTGTGATGATGAAATTTGCAAATACACCACACGAAGTCTCAACCTCCGGCTTGTTGGTGATTCTGAGAGAGGAACAGTCTGTCCAAACTATCCCCACTGCAATGGGCGTCTTGTAAGGAAGTACACGGAAGCTGATCTATACAAGCAGCTCTCATTTTTCTGTCACATCTTGGATACAGTACGTTGCATAGAAAAGATGGAACCTAAGACCAGACTGCTGGTCGAGCTAGAGTTGGCCAAAATTCGCCCAATTGTGACTTTGGCTGCCTCAACAGTTGAAAGAATTCGGGACCGTTGTGCCTACGGGTGGATACAGCTGCAAGATCTTTTTGTTACAGTTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000228 GO:0000428 GO:0000726 GO:0003674 GO:0003824 GO:0003887 GO:0003896 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005657 GO:0005658 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006269 GO:0006270 GO:0006271 GO:0006272 GO:0006273 GO:0006281 GO:0006302 GO:0006303 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009653 GO:0009965 GO:0009987 GO:0010016 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019438 GO:0022616 GO:0030880 GO:0030894 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032774 GO:0032991 GO:0032993 GO:0033554 GO:0034061 GO:0034062 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043596 GO:0043601 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0046483 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0051716 GO:0055029 GO:0061695 GO:0070013 GO:0071704 GO:0071897 GO:0090304 GO:0097747 GO:0099402 GO:0140097 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1905392 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko01100 ko03030 map00230 map00240 map01100 map03030

Pfam Domains

Protein Families

DNA_pol_B DNA_pol_B_exo1 DNA_pol_alpha_N zf-DNA_Pol

Protein Analysis

1533

Amino Acids

171.12

Weight (kDa)

7.92

Isoelectric Point (pI)

44.06

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DNA_pol_alpha_N	PF12254	26 - 95	1.6e-19	DNA polymerase alpha subunit p180 N terminal
DNA_pol_B_exo1	PF03104	504 - 757	3.8e-25	DNA polymerase family B, exonuclease domain
DNA_pol_B	PF00136	823 - 1285	7.4e-128	DNA polymerase family B
zf-DNA_Pol	PF08996	1323 - 1405	1.6e-06	DNA Polymerase alpha zinc finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0000310)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G67100 AT5G67100
fragaria_vesca	FvH4_1g20001 FvH4_1g22730 FvH4_1g22730 FvH4_1g22730 FvH4_6g13861 FvH4_6g33603
malus_domestica	MD02G1174100.v1.1 MD02G1174200.v1.1 MD02G1174400.v1.1 MD15G1285400.v1.1
prunus_persica	Prupe.7G131600_v2.0.a1
pyrus_communis	pycom02g14040 pycom02g14050 pycom02g14060 pycom02g14070 pycom15g24830 pycom15g24840
rosa_chinensis	RchiOBHm_Chr2g0109901 RchiOBHm_Chr2g0117021 RchiOBHm_Chr2g0168121 RchiOBHm_Chr4g0394581
rosa_laevigata	RLG00000001621 RLG00000001943 RLG00000003450 RLG00000006116 RLG00000006535 RLG00000008147 RLG00000009121 RLG00000010680 RLG00000013987 RLG00000017585 RLG00000017603 RLG00000017638 RLG00000017923 RLG00000018320 RLG00000021520 RLG00000022613 RLG00000022614 RLG00000023966 RLG00000025998 RLG00000027760 RLG00000033421
rosa_multiflora	Rmu_co7985806.1_g000001 Rmu_co8250293.1_g000001 Rmu_co8353517.1_g000001 Rmu_sc0000697.1_g000011 Rmu_sc0000776.1_g000079 Rmu_sc0001023.1_g000057 Rmu_sc0001266.1_g000019 Rmu_sc0002711.1_g000010 Rmu_sc0002731.1_g000005 Rmu_sc0003002.1_g000047 Rmu_sc0003030.1_g000002 Rmu_sc0003672.1_g000003 Rmu_sc0004168.1_g000062 Rmu_sc0004180.1_g000003 Rmu_sc0009205.1_g000008 Rmu_sc0011259.1_g000001 Rmu_sc0011259.1_g000002 Rmu_sc0027274.1_g000001
rosa_roxburghii	Rroxscaffold_1G00023520 Rroxscaffold_2G00104450 Rroxscaffold_2G00126810 Rroxscaffold_2G00144870 Rroxscaffold_4G00315680 Rroxscaffold_4G00331480 Rroxscaffold_7G00182820
rosa_rugosa	Rorug02G0209600 Rorug04G0130600 Rorug04G0130600 Rorug04G0130700 Rorug05G0230000
rosa_samantha	Rh1AG170000 Rh1CG157700 Rh1CG385600 Rh2AG215100 Rh2AG265500 Rh2BG277300 Rh2CG217600 Rh2CG217700 Rh2DG220600 Rh2DG272800 Rh2DG291800 Rh3AG215700 Rh3BG271700 Rh3DG075200 Rh3DG242600 Rh4AG115400 Rh4AG138100 Rh4AG333600 Rh4BG115100 Rh4BG366300 Rh4CG125400 Rh4CG380400 Rh4DG083500 Rh4DG113200 Rh4DG192900 Rh4DG246300 Rh5AG075000 Rh5BG482300 Rh5CG181200 Rh5CG247200 Rh5CG506900 Rh5DG167600 Rh5DG202900 Rh6AG000500 Rh6AG213400 Rh6BG335800 Rh6CG255700 Rh7AG337500 Rh7BG404500 Rh7BG461500 Rh7CG355600
rosa_wichuraiana	Rw0G019110 Rw2G020950

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	3080
Acc65I	GGTACC	2 cut(s)	255, 3860
AccB1I	GGYRCC	3 cut(s)	255, 802, 3860
AccB7I	CCANNNNNTGG	2 cut(s)	2678, 3877
AccBSI	CCGCTC	1 cut(s)	114
AccI	GTMKAC	1 cut(s)	174
AccII	CGCG	6 cut(s)	64, 194, 213, 261, 294, 401
AccIII	TCCGGA	1 cut(s)	2554
AcoI	YGGCCR	3 cut(s)	106, 927, 4491
AcsI	RAATTY	9 cut(s)	1576, 1619, 1918, 2839, 3043, 3712, 4269, 4497, 4539
AcuI	CTGAAG	3 cut(s)	1284, 1448, 3020
AcyI	GRCGYC	1 cut(s)	4363
AdeI	CACNNNGTG	1 cut(s)	2678
AflIII	ACRYGT	4 cut(s)	399, 2438, 3613, 4256
AgeI	ACCGGT	1 cut(s)	132
AhdI	GACNNNNNGTC	1 cut(s)	4475
AjiI	CACGTC	3 cut(s)	459, 2441, 4257
AjnI	CCWGG	5 cut(s)	75, 805, 1831, 2179, 2881
AleI	CACNNNNGTG	1 cut(s)	1805
AloI	GAACNNNNNNTCC	2 cut(s)	4321, 4353
Alw21I	GWGCWC	1 cut(s)	2071
Alw26I	GTCTC	8 cut(s)	89, 1663, 1765, 2375, 2872, 2966, 4185, 4298
Ama87I	CYCGRG	2 cut(s)	115, 381
Aor13HI	TCCGGA	1 cut(s)	2554
ApoI	RAATTY	9 cut(s)	1576, 1619, 1918, 2839, 3043, 3712, 4269, 4497, 4539
AsiGI	ACCGGT	1 cut(s)	132
Asp700I	GAANNNNTTC	4 cut(s)	599, 3486, 3943, 4539
Asp718I	GGTACC	2 cut(s)	255, 3860
AspA2I	CCTAGG	1 cut(s)	250
AspLEI	GCGC	8 cut(s)	64, 74, 210, 550, 586, 1517, 3628, 4140
AspS9I	GGNCC	8 cut(s)	66, 1085, 1553, 2100, 2293, 2702, 3379, 4546
AsuC2I	CCSGG	2 cut(s)	1448, 3730
AsuII	TTCGAA	1 cut(s)	1276
AvaI	CYCGRG	2 cut(s)	115, 381
AvaII	GGWCC	6 cut(s)	66, 1085, 1553, 2100, 2702, 4546
AvrII	CCTAGG	1 cut(s)	250
BaeGI	GKGCMC	1 cut(s)	2500
BalI	TGGCCA	1 cut(s)	4493
BamHI	GGATCC	1 cut(s)	1450
BanI	GGYRCC	3 cut(s)	255, 802, 3860
BanII	GRGCYC	1 cut(s)	2071
BarI	GAAGNNNNNNTAC	2 cut(s)	146, 178
BbsI	GAAGAC	3 cut(s)	824, 873, 3984
Bbv12I	GWGCWC	1 cut(s)	2071
BbvCI	CCTCAGC	1 cut(s)	2631
BccI	CCATC	9 cut(s)	421, 479, 927, 1031, 1865, 3590, 3770, 4058, 4447
BceAI	ACGGC	5 cut(s)	262, 283, 340, 426, 3295
BcgI	CGANNNNNNTGC	2 cut(s)	2369, 2403
BciT130I	CCWGG	5 cut(s)	77, 807, 1833, 2181, 2883
BciVI	GTATCC	3 cut(s)	2572, 4423, 4560
BcnI	CCSGG	2 cut(s)	1448, 3730
BcoDI	GTCTC	8 cut(s)	89, 1663, 1765, 2375, 2872, 2966, 4185, 4298
BfmI	CTRYAG	1 cut(s)	1680
BfoI	RGCGCY	2 cut(s)	75, 211
BfuI	GTATCC	3 cut(s)	2572, 4423, 4560
BglI	GCCNNNNNGGC	2 cut(s)	56, 4093
BglII	AGATCT	2 cut(s)	1468, 4579
BlnI	CCTAGG	1 cut(s)	250
BlpI	GCTNAGC	1 cut(s)	1841
BmcAI	AGTACT	1 cut(s)	3112
Bme1390I	CCNGG	7 cut(s)	77, 807, 1448, 1833, 2181, 2883, 3730
Bme18I	GGWCC	6 cut(s)	66, 1085, 1553, 2100, 2702, 4546
BmeRI	GACNNNNNGTC	1 cut(s)	4475
BmeT110I	CYCGRG	2 cut(s)	115, 381
BmgBI	CACGTC	3 cut(s)	459, 2441, 4257
BmgT120I	GGNCC	8 cut(s)	66, 1085, 1553, 2100, 2293, 2702, 3379, 4546
BmrFI	CCNGG	7 cut(s)	77, 807, 1448, 1833, 2181, 2883, 3730
BmrI	ACTGGG	1 cut(s)	984
BmsI	GCATC	8 cut(s)	43, 319, 1054, 1775, 2121, 2377, 2963, 3017
BmuI	ACTGGG	1 cut(s)	984
BoxI	GACNNNNGTC	1 cut(s)	1367
BpiI	GAAGAC	3 cut(s)	824, 873, 3984
BpmI	CTGGAG	5 cut(s)	98, 170, 1929, 2190, 3424
Bpu10I	CCTNAGC	3 cut(s)	2070, 2136, 2631
Bpu1102I	GCTNAGC	1 cut(s)	1841
Bpu14I	TTCGAA	1 cut(s)	1276
BpuEI	CTTGAG	5 cut(s)	1963, 2102, 2948, 3290, 3563
BpuMI	CCSGG	2 cut(s)	1448, 3730
BsaBI	GATNNNNATC	1 cut(s)	1023
BsaHI	GRCGYC	1 cut(s)	4363
BsaI	GGTCTC	3 cut(s)	1663, 2872, 2966
BsaJI	CCNNGG	8 cut(s)	43, 75, 250, 292, 380, 1887, 2180, 2485
BsaWI	WCCGGW	3 cut(s)	132, 2301, 2554
Bse118I	RCCGGY	2 cut(s)	132, 589
Bse1I	ACTGG	8 cut(s)	287, 955, 979, 1406, 3407, 3661, 3864, 4116
Bse3DI	GCAATG	3 cut(s)	2196, 4062, 4363
Bse8I	GATNNNNATC	1 cut(s)	1023
BseAI	TCCGGA	1 cut(s)	2554
BseBI	CCWGG	5 cut(s)	77, 807, 1833, 2181, 2883
BseDI	CCNNGG	8 cut(s)	43, 75, 250, 292, 380, 1887, 2180, 2485
BseJI	GATNNNNATC	1 cut(s)	1023
BseMI	GCAATG	3 cut(s)	2196, 4062, 4363
BseNI	ACTGG	8 cut(s)	287, 955, 979, 1406, 3407, 3661, 3864, 4116
BseRI	GAGGAG	4 cut(s)	60, 230, 2648, 2938
BseSI	GKGCMC	1 cut(s)	2500
BseX3I	CGGCCG	1 cut(s)	106
BseYI	CCCAGC	3 cut(s)	965, 1591, 2290
BsgI	GTGCAG	1 cut(s)	4150
Bsh1236I	CGCG	6 cut(s)	64, 194, 213, 261, 294, 401
Bsh1285I	CGRYCG	3 cut(s)	24, 109, 133
BshNI	GGYRCC	3 cut(s)	255, 802, 3860
BshTI	ACCGGT	1 cut(s)	132
BsiEI	CGRYCG	3 cut(s)	24, 109, 133
BsiHKAI	GWGCWC	1 cut(s)	2071
BsiHKCI	CYCGRG	2 cut(s)	115, 381
BslFI	GGGAC	5 cut(s)	239, 2418, 3796, 3852, 4559
BsmAI	GTCTC	8 cut(s)	89, 1663, 1765, 2375, 2872, 2966, 4185, 4298
BsmBI	CGTCTC	1 cut(s)	89
BsmFI	GGGAC	5 cut(s)	239, 2418, 3796, 3852, 4559
BsmI	GAATGC	3 cut(s)	889, 2956, 3300
Bso31I	GGTCTC	3 cut(s)	1663, 2872, 2966
BsoBI	CYCGRG	2 cut(s)	115, 381
Bsp119I	TTCGAA	1 cut(s)	1276
Bsp1286I	GDGCHC	2 cut(s)	2071, 2500
Bsp13I	TCCGGA	1 cut(s)	2554
Bsp1407I	TGTACA	1 cut(s)	3285
Bsp1720I	GCTNAGC	1 cut(s)	1841
Bsp19I	CCATGG	1 cut(s)	1887
BspEI	TCCGGA	1 cut(s)	2554
BspFNI	CGCG	6 cut(s)	64, 194, 213, 261, 294, 401
BspQI	GCTCTTC	1 cut(s)	1500
BspT104I	TTCGAA	1 cut(s)	1276
BspT107I	GGYRCC	3 cut(s)	255, 802, 3860
BspTNI	GGTCTC	3 cut(s)	1663, 2872, 2966
BsrBI	CCGCTC	1 cut(s)	114
BsrDI	GCAATG	3 cut(s)	2196, 4062, 4363
BsrFI	RCCGGY	2 cut(s)	132, 589
BsrGI	TGTACA	1 cut(s)	3285
BsrI	ACTGG	8 cut(s)	287, 955, 979, 1406, 3407, 3661, 3864, 4116
BssAI	RCCGGY	2 cut(s)	132, 589
BssECI	CCNNGG	8 cut(s)	43, 75, 250, 292, 380, 1887, 2180, 2485
BssNI	GRCGYC	1 cut(s)	4363
BssT1I	CCWWGG	3 cut(s)	250, 1887, 2485
Bst2UI	CCWGG	5 cut(s)	77, 807, 1833, 2181, 2883
Bst6I	CTCTTC	7 cut(s)	270, 335, 590, 1068, 1242, 1500, 1820
BstACI	GRCGYC	1 cut(s)	4363
BstAPI	GCANNNNNTGC	1 cut(s)	920
BstAUI	TGTACA	1 cut(s)	3285
BstBI	TTCGAA	1 cut(s)	1276
BstC8I	GCNNGC	4 cut(s)	296, 2142, 2994, 4215
BstDSI	CCRYGG	2 cut(s)	292, 1887
BstFNI	CGCG	6 cut(s)	64, 194, 213, 261, 294, 401
BstH2I	RGCGCY	2 cut(s)	75, 211
BstHHI	GCGC	8 cut(s)	64, 74, 210, 550, 586, 1517, 3628, 4140
BstMAI	GTCTC	8 cut(s)	89, 1663, 1765, 2375, 2872, 2966, 4185, 4298
BstMCI	CGRYCG	3 cut(s)	24, 109, 133
BstNI	CCWGG	5 cut(s)	77, 807, 1833, 2181, 2883
BstNSI	RCATGY	5 cut(s)	1060, 1171, 2098, 3617, 4017
BstPAI	GACNNNNGTC	1 cut(s)	1367
BstSCI	CCNGG	7 cut(s)	75, 805, 1446, 1831, 2179, 2881, 3728
BstSFI	CTRYAG	1 cut(s)	1680
BstSLI	GKGCMC	1 cut(s)	2500
BstUI	CGCG	6 cut(s)	64, 194, 213, 261, 294, 401
BstV2I	GAAGAC	3 cut(s)	824, 873, 3984
BstX2I	RGATCY	6 cut(s)	1336, 1450, 1468, 3099, 3118, 4579
BstXI	CCANNNNNNTGG	3 cut(s)	76, 4358, 4474
BstYI	RGATCY	6 cut(s)	1336, 1450, 1468, 3099, 3118, 4579
BstZI	CGGCCG	1 cut(s)	106
BsuI	GTATCC	3 cut(s)	2572, 4423, 4560
BtgI	CCRYGG	2 cut(s)	292, 1887
BtgZI	GCGATG	3 cut(s)	342, 437, 959
BtrI	CACGTC	3 cut(s)	459, 2441, 4257
BtsI	GCAGTG	3 cut(s)	1043, 1963, 4351
BtsIMutI	CAGTG	6 cut(s)	991, 1043, 1963, 4084, 4123, 4351
Cac8I	GCNNGC	4 cut(s)	296, 2142, 2994, 4215
CciNI	GCGGCCGC	1 cut(s)	106
CfoI	GCGC	8 cut(s)	64, 74, 210, 550, 586, 1517, 3628, 4140
Cfr10I	RCCGGY	2 cut(s)	132, 589
Cfr13I	GGNCC	8 cut(s)	66, 1085, 1553, 2100, 2293, 2702, 3379, 4546
Cfr42I	CCGCGG	1 cut(s)	295
CseI	GACGC	5 cut(s)	71, 200, 390, 1301, 4352
CspAI	ACCGGT	1 cut(s)	132
CspCI	CAANNNNNGTGG	4 cut(s)	2440, 2475, 3405, 3440
DraIII	CACNNNGTG	1 cut(s)	2678
DriI	GACNNNNNGTC	1 cut(s)	4475
EaeI	YGGCCR	3 cut(s)	106, 927, 4491
EagI	CGGCCG	1 cut(s)	106
Eam1104I	CTCTTC	7 cut(s)	270, 335, 590, 1068, 1242, 1500, 1820
Eam1105I	GACNNNNNGTC	1 cut(s)	4475
EarI	CTCTTC	7 cut(s)	270, 335, 590, 1068, 1242, 1500, 1820
EciI	GGCGGA	3 cut(s)	20, 592, 860
Ecl136II	GAGCTC	1 cut(s)	2069
EclXI	CGGCCG	1 cut(s)	106
Eco130I	CCWWGG	3 cut(s)	250, 1887, 2485
Eco24I	GRGCYC	1 cut(s)	2071
Eco31I	GGTCTC	3 cut(s)	1663, 2872, 2966
Eco32I	GATATC	2 cut(s)	1687, 3986
Eco47I	GGWCC	6 cut(s)	66, 1085, 1553, 2100, 2702, 4546
Eco52I	CGGCCG	1 cut(s)	106
Eco53kI	GAGCTC	1 cut(s)	2069
Eco57I	CTGAAG	3 cut(s)	1284, 1448, 3020
Eco88I	CYCGRG	2 cut(s)	115, 381
EcoICRI	GAGCTC	1 cut(s)	2069
EcoO109I	RGGNCCY	3 cut(s)	1553, 2702, 3379
EcoRI	GAATTC	3 cut(s)	1918, 3712, 4539
EcoRII	CCWGG	5 cut(s)	75, 805, 1831, 2179, 2881
EcoRV	GATATC	2 cut(s)	1687, 3986
EcoT14I	CCWWGG	3 cut(s)	250, 1887, 2485
EcoT22I	ATGCAT	2 cut(s)	2392, 2956
EcoT38I	GRGCYC	1 cut(s)	2071
ErhI	CCWWGG	3 cut(s)	250, 1887, 2485
Esp3I	CGTCTC	1 cut(s)	89
FalI	AAGNNNNNCTT	2 cut(s)	3525, 3557
FaqI	GGGAC	5 cut(s)	239, 2418, 3796, 3852, 4559
FauI	CCCGC	2 cut(s)	20, 287
FauNDI	CATATG	1 cut(s)	2571
FblI	GTMKAC	1 cut(s)	174
FriOI	GRGCYC	1 cut(s)	2071
GlaI	GCGC	8 cut(s)	63, 73, 209, 549, 585, 1516, 3627, 4139
GsaI	CCCAGC	3 cut(s)	969, 1595, 2294
GsuI	CTGGAG	5 cut(s)	98, 170, 1929, 2190, 3424
HaeII	RGCGCY	2 cut(s)	75, 211
HgaI	GACGC	5 cut(s)	71, 200, 390, 1301, 4352
HhaI	GCGC	8 cut(s)	64, 74, 210, 550, 586, 1517, 3628, 4140
Hin1I	GRCGYC	1 cut(s)	4363
Hin6I	GCGC	8 cut(s)	62, 72, 208, 548, 584, 1515, 3626, 4138
HinP1I	GCGC	8 cut(s)	62, 72, 208, 548, 584, 1515, 3626, 4138
HincII	GTYRAC	3 cut(s)	1123, 1750, 3244
HindII	GTYRAC	3 cut(s)	1123, 1750, 3244
HindIII	AAGCTT	2 cut(s)	1865, 2138
HpaI	GTTAAC	3 cut(s)	1123, 1750, 3244
Hpy166II	GTNNAC	7 cut(s)	175, 456, 1123, 1750, 1896, 3244, 4380
Hpy8I	GTNNAC	7 cut(s)	175, 456, 1123, 1750, 1896, 3244, 4380
Hsp92I	GRCGYC	1 cut(s)	4363
HspAI	GCGC	8 cut(s)	62, 72, 208, 548, 584, 1515, 3626, 4138
Kpn2I	TCCGGA	1 cut(s)	2554
KpnI	GGTACC	2 cut(s)	259, 3864
KspAI	GTTAAC	3 cut(s)	1123, 1750, 3244
KspI	CCGCGG	1 cut(s)	295
LguI	GCTCTTC	1 cut(s)	1500
LweI	GCATC	8 cut(s)	43, 319, 1054, 1775, 2121, 2377, 2963, 3017
MbiI	CCGCTC	1 cut(s)	114
MfeI	CAATTG	3 cut(s)	773, 3216, 4506
MflI	RGATCY	6 cut(s)	1336, 1450, 1468, 3099, 3118, 4579
MhlI	GDGCHC	2 cut(s)	2071, 2500
MlsI	TGGCCA	1 cut(s)	4493
MluI	ACGCGT	1 cut(s)	399
MluNI	TGGCCA	1 cut(s)	4493
MlyI	GAGTC	6 cut(s)	1259, 1808, 1837, 2278, 2400, 2514
MmeI	TCCRAC	6 cut(s)	150, 291, 1314, 1983, 2002, 2619
Mox20I	TGGCCA	1 cut(s)	4493
Mph1103I	ATGCAT	2 cut(s)	2392, 2956
MroI	TCCGGA	1 cut(s)	2554
MroXI	GAANNNNTTC	4 cut(s)	599, 3486, 3943, 4539
MscI	TGGCCA	1 cut(s)	4493
MslI	CAYNNNNRTG	5 cut(s)	1805, 2227, 2421, 3756, 4356
Msp20I	TGGCCA	1 cut(s)	4493
MspA1I	CMGCKG	6 cut(s)	294, 439, 2290, 2446, 3326, 4573
MspR9I	CCNGG	7 cut(s)	77, 807, 1448, 1833, 2181, 2883, 3730
MunI	CAATTG	3 cut(s)	773, 3216, 4506
Mva1269I	GAATGC	3 cut(s)	889, 2956, 3300
MvaI	CCWGG	5 cut(s)	77, 807, 1833, 2181, 2883
MvnI	CGCG	6 cut(s)	64, 194, 213, 261, 294, 401
NciI	CCSGG	2 cut(s)	1448, 3730
NcoI	CCATGG	1 cut(s)	1887
NdeI	CATATG	1 cut(s)	2571
NmeAIII	GCCGAG	1 cut(s)	68
NmuCI	GTSAC	6 cut(s)	488, 1041, 2237, 3398, 4418, 4510
NotI	GCGGCCGC	1 cut(s)	106
NsiI	ATGCAT	2 cut(s)	2392, 2956
NspI	RCATGY	5 cut(s)	1060, 1171, 2098, 3617, 4017
NspV	TTCGAA	1 cut(s)	1276
OliI	CACNNNNGTG	1 cut(s)	1805
PciI	ACATGT	1 cut(s)	3613
PciSI	GCTCTTC	1 cut(s)	1500
PcsI	WCGNNNNNNNCGW	2 cut(s)	240, 455
PctI	GAATGC	3 cut(s)	889, 2956, 3300
PdmI	GAANNNNTTC	4 cut(s)	599, 3486, 3943, 4539
PflMI	CCANNNNNTGG	2 cut(s)	2678, 3877
PfoI	TCCNGGA	1 cut(s)	1446
PinAI	ACCGGT	1 cut(s)	132
Ple19I	CGATCG	1 cut(s)	24
PleI	GAGTC	6 cut(s)	1258, 1808, 1836, 2278, 2400, 2513
PpsI	GAGTC	6 cut(s)	1258, 1808, 1836, 2278, 2400, 2513
PpuMI	RGGWCCY	2 cut(s)	1553, 2702
PscI	ACATGT	1 cut(s)	3613
PshAI	GACNNNNGTC	1 cut(s)	1367
PsiI	TTATAA	1 cut(s)	3080
Psp124BI	GAGCTC	1 cut(s)	2071
Psp5II	RGGWCCY	2 cut(s)	1553, 2702
Psp6I	CCWGG	5 cut(s)	75, 805, 1831, 2179, 2881
PspFI	CCCAGC	3 cut(s)	965, 1591, 2290
PspGI	CCWGG	5 cut(s)	75, 805, 1831, 2179, 2881
PspPI	GGNCC	8 cut(s)	66, 1085, 1553, 2100, 2293, 2702, 3379, 4546
PspPPI	RGGWCCY	2 cut(s)	1553, 2702
PsuI	RGATCY	6 cut(s)	1336, 1450, 1468, 3099, 3118, 4579
PvuI	CGATCG	1 cut(s)	24
PvuII	CAGCTG	4 cut(s)	2290, 2446, 3326, 4573
RseI	CAYNNNNRTG	5 cut(s)	1805, 2227, 2421, 3756, 4356
SacI	GAGCTC	1 cut(s)	2071
SacII	CCGCGG	1 cut(s)	295
SapI	GCTCTTC	1 cut(s)	1500
Sau96I	GGNCC	8 cut(s)	66, 1085, 1553, 2100, 2293, 2702, 3379, 4546
ScaI	AGTACT	1 cut(s)	3112
SchI	GAGTC	6 cut(s)	1259, 1808, 1837, 2278, 2400, 2514
ScrFI	CCNGG	7 cut(s)	77, 807, 1448, 1833, 2181, 2883, 3730
SduI	GDGCHC	2 cut(s)	2071, 2500
SfaNI	GCATC	8 cut(s)	43, 319, 1054, 1775, 2121, 2377, 2963, 3017
SfcI	CTRYAG	1 cut(s)	1680
Sfr303I	CCGCGG	1 cut(s)	295
SfuI	TTCGAA	1 cut(s)	1276
SgrBI	CCGCGG	1 cut(s)	295
SinI	GGWCC	6 cut(s)	66, 1085, 1553, 2100, 2702, 4546
SmiMI	CAYNNNNRTG	5 cut(s)	1805, 2227, 2421, 3756, 4356
SmlI	CTYRAG	5 cut(s)	1942, 2117, 2963, 3305, 3542
SmoI	CTYRAG	5 cut(s)	1942, 2117, 2963, 3305, 3542
SspI	AATATT	2 cut(s)	2455, 3450
SstI	GAGCTC	1 cut(s)	2071
StyD4I	CCNGG	7 cut(s)	75, 805, 1446, 1831, 2179, 2881, 3728
StyI	CCWWGG	3 cut(s)	250, 1887, 2485
TaqI	TCGA	8 cut(s)	234, 449, 679, 1233, 1276, 2508, 2916, 4479
TatI	WGTACW	5 cut(s)	1423, 3110, 3285, 4236, 4377
TscAI	CASTG	6 cut(s)	991, 1050, 1963, 4084, 4123, 4358
TseFI	GTSAC	6 cut(s)	488, 1041, 2237, 3398, 4418, 4510
Tsp45I	GTSAC	6 cut(s)	488, 1041, 2237, 3398, 4418, 4510
TspGWI	ACGGA	5 cut(s)	52, 86, 1850, 2611, 4397
TspRI	CASTG	6 cut(s)	991, 1050, 1963, 4084, 4123, 4358
Van91I	CCANNNNNTGG	2 cut(s)	2678, 3877
VpaK11BI	GGWCC	6 cut(s)	66, 1085, 1553, 2100, 2702, 4546
XapI	RAATTY	9 cut(s)	1576, 1619, 1918, 2839, 3043, 3712, 4269, 4497, 4539
XceI	RCATGY	5 cut(s)	1060, 1171, 2098, 3617, 4017
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2094
XmaJI	CCTAGG	1 cut(s)	250
XmiI	GTMKAC	1 cut(s)	174
XmnI	GAANNNNTTC	4 cut(s)	599, 3486, 3943, 4539
ZrmI	AGTACT	1 cut(s)	3112
Zsp2I	ATGCAT	2 cut(s)	2392, 2956

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.