DNA polymerase
| Domain Name | Pfam ID | Position | E-value | Description |
|---|---|---|---|---|
| DNA_pol_alpha_N | PF12254 | 2 - 46 | 2.2e-08 | DNA polymerase alpha subunit p180 N terminal |
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 27 |
| AccII | CGCG | 3 cut(s) | 147, 149, 238 |
| AciI | CCGC | 9 cut(s) | 145, 236, 238, 269, 272, 275, 430, 446, 500 |
| AcuI | CTGAAG | 1 cut(s) | 67 |
| AcyI | GRCGYC | 1 cut(s) | 45 |
| AfaI | GTAC | 1 cut(s) | 41 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 124, 198, 470 |
| AgsI | TTSAA | 2 cut(s) | 538, 551 |
| AjiI | CACGTC | 1 cut(s) | 315 |
| AjnI | CCWGG | 2 cut(s) | 191, 558 |
| AoxI | GGCC | 4 cut(s) | 100, 188, 239, 351 |
| AspA2I | CCTAGG | 1 cut(s) | 103 |
| AspLEI | GCGC | 3 cut(s) | 149, 151, 406 |
| AspS9I | GGNCC | 3 cut(s) | 138, 189, 240 |
| AsuHPI | GGTGA | 3 cut(s) | 46, 289, 356 |
| AvaII | GGWCC | 1 cut(s) | 138 |
| AvrII | CCTAGG | 1 cut(s) | 103 |
| BaeGI | GKGCMC | 1 cut(s) | 442 |
| BbsI | GAAGAC | 1 cut(s) | 209 |
| BccI | CCATC | 4 cut(s) | 170, 277, 335, 506 |
| BceAI | ACGGC | 2 cut(s) | 115, 136 |
| BciT130I | CCWGG | 2 cut(s) | 193, 560 |
| BfaI | CTAG | 1 cut(s) | 104 |
| BisI | GCNGC | 6 cut(s) | 145, 236, 239, 270, 273, 446 |
| BlnI | CCTAGG | 1 cut(s) | 103 |
| BlsI | GCNGC | 6 cut(s) | 146, 237, 240, 271, 274, 447 |
| Bme1390I | CCNGG | 2 cut(s) | 193, 560 |
| Bme18I | GGWCC | 1 cut(s) | 138 |
| BmgBI | CACGTC | 1 cut(s) | 315 |
| BmgT120I | GGNCC | 3 cut(s) | 138, 189, 240 |
| BmiI | GGNNCC | 1 cut(s) | 234 |
| BmrFI | CCNGG | 2 cut(s) | 193, 560 |
| BpiI | GAAGAC | 1 cut(s) | 209 |
| BsaHI | GRCGYC | 1 cut(s) | 45 |
| BsaJI | CCNNGG | 5 cut(s) | 103, 118, 191, 236, 449 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 124, 198, 470 |
| Bse1I | ACTGG | 1 cut(s) | 140 |
| BseBI | CCWGG | 2 cut(s) | 193, 560 |
| BseDI | CCNNGG | 5 cut(s) | 103, 118, 191, 236, 449 |
| BseGI | GGATG | 4 cut(s) | 172, 181, 346, 388 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 124, 198, 470 |
| BseNI | ACTGG | 1 cut(s) | 140 |
| BsePI | GCGCGC | 1 cut(s) | 147 |
| BseRI | GAGGAG | 4 cut(s) | 83, 211, 448, 466 |
| BseSI | GKGCMC | 1 cut(s) | 442 |
| Bsh1236I | CGCG | 3 cut(s) | 147, 149, 238 |
| BshFI | GGCC | 4 cut(s) | 102, 190, 241, 353 |
| BsiSI | CCGG | 1 cut(s) | 407 |
| BslFI | GGGAC | 1 cut(s) | 92 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 124, 198, 470 |
| BsmFI | GGGAC | 1 cut(s) | 92 |
| BsnI | GGCC | 4 cut(s) | 102, 190, 241, 353 |
| Bsp1286I | GDGCHC | 1 cut(s) | 442 |
| Bsp143I | GATC | 1 cut(s) | 12 |
| BspACI | CCGC | 9 cut(s) | 145, 236, 238, 269, 272, 275, 430, 446, 500 |
| BspANI | GGCC | 4 cut(s) | 102, 190, 241, 353 |
| BspFNI | CGCG | 3 cut(s) | 147, 149, 238 |
| BspLI | GGNNCC | 1 cut(s) | 234 |
| BsrI | ACTGG | 1 cut(s) | 140 |
| BssECI | CCNNGG | 5 cut(s) | 103, 118, 191, 236, 449 |
| BssHII | GCGCGC | 1 cut(s) | 147 |
| BssMI | GATC | 1 cut(s) | 12 |
| BssNI | GRCGYC | 1 cut(s) | 45 |
| BssT1I | CCWWGG | 1 cut(s) | 103 |
| Bst2UI | CCWGG | 2 cut(s) | 193, 560 |
| Bst4CI | ACNGT | 2 cut(s) | 25, 208 |
| Bst6I | CTCTTC | 1 cut(s) | 123 |
| BstACI | GRCGYC | 1 cut(s) | 45 |
| BstC8I | GCNNGC | 1 cut(s) | 149 |
| BstDSI | CCRYGG | 2 cut(s) | 118, 236 |
| BstF5I | GGATG | 4 cut(s) | 172, 181, 346, 388 |
| BstFNI | CGCG | 3 cut(s) | 147, 149, 238 |
| BstHHI | GCGC | 3 cut(s) | 149, 151, 406 |
| BstKTI | GATC | 1 cut(s) | 15 |
| BstMBI | GATC | 1 cut(s) | 12 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 120, 401 |
| BstNI | CCWGG | 2 cut(s) | 193, 560 |
| BstNSI | RCATGY | 1 cut(s) | 509 |
| BstSCI | CCNGG | 2 cut(s) | 191, 558 |
| BstSLI | GKGCMC | 1 cut(s) | 442 |
| BstUI | CGCG | 3 cut(s) | 147, 149, 238 |
| BstV2I | GAAGAC | 1 cut(s) | 209 |
| BsuRI | GGCC | 4 cut(s) | 102, 190, 241, 353 |
| BtgI | CCRYGG | 2 cut(s) | 118, 236 |
| BtrI | CACGTC | 1 cut(s) | 315 |
| BtsCI | GGATG | 4 cut(s) | 172, 181, 346, 388 |
| Cac8I | GCNNGC | 1 cut(s) | 149 |
| CfoI | GCGC | 3 cut(s) | 149, 151, 406 |
| Cfr13I | GGNCC | 3 cut(s) | 138, 189, 240 |
| Cfr42I | CCGCGG | 1 cut(s) | 239 |
| CseI | GACGC | 1 cut(s) | 53 |
| Csp6I | GTAC | 1 cut(s) | 40 |
| CviAII | CATG | 1 cut(s) | 506 |
| CviQI | GTAC | 1 cut(s) | 40 |
| DpnI | GATC | 1 cut(s) | 14 |
| DpnII | GATC | 1 cut(s) | 12 |
| Eam1104I | CTCTTC | 1 cut(s) | 123 |
| EarI | CTCTTC | 1 cut(s) | 123 |
| EciI | GGCGGA | 1 cut(s) | 445 |
| Eco130I | CCWWGG | 1 cut(s) | 103 |
| Eco47I | GGWCC | 1 cut(s) | 138 |
| Eco57I | CTGAAG | 1 cut(s) | 67 |
| EcoRII | CCWGG | 2 cut(s) | 191, 558 |
| EcoT14I | CCWWGG | 1 cut(s) | 103 |
| ErhI | CCWWGG | 1 cut(s) | 103 |
| FaeI | CATG | 1 cut(s) | 509 |
| FaiI | YATR | 5 cut(s) | 18, 185, 507, 555, 571 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 349, 381 |
| FaqI | GGGAC | 1 cut(s) | 92 |
| FatI | CATG | 1 cut(s) | 505 |
| FblI | GTMKAC | 1 cut(s) | 27 |
| Fnu4HI | GCNGC | 6 cut(s) | 145, 236, 239, 270, 273, 446 |
| FokI | GGATG | 4 cut(s) | 179, 188, 353, 395 |
| Fsp4HI | GCNGC | 6 cut(s) | 145, 236, 239, 270, 273, 446 |
| FspBI | CTAG | 1 cut(s) | 104 |
| GlaI | GCGC | 3 cut(s) | 148, 150, 405 |
| GluI | GCNGC | 6 cut(s) | 145, 236, 239, 270, 273, 446 |
| HaeIII | GGCC | 4 cut(s) | 102, 190, 241, 353 |
| HapII | CCGG | 1 cut(s) | 407 |
| HgaI | GACGC | 1 cut(s) | 53 |
| HhaI | GCGC | 3 cut(s) | 149, 151, 406 |
| Hin1I | GRCGYC | 1 cut(s) | 45 |
| Hin1II | CATG | 1 cut(s) | 509 |
| Hin6I | GCGC | 3 cut(s) | 147, 149, 404 |
| HinP1I | GCGC | 3 cut(s) | 147, 149, 404 |
| HinfI | GANTC | 1 cut(s) | 170 |
| HpaII | CCGG | 1 cut(s) | 407 |
| HphI | GGTGA | 3 cut(s) | 46, 289, 356 |
| Hpy166II | GTNNAC | 2 cut(s) | 28, 312 |
| Hpy188I | TCNGA | 4 cut(s) | 156, 166, 175, 465 |
| Hpy188III | TCNNGA | 1 cut(s) | 535 |
| Hpy8I | GTNNAC | 2 cut(s) | 28, 312 |
| Hpy99I | CGWCG | 5 cut(s) | 47, 98, 101, 319, 322 |
| HpyAV | CCTTC | 4 cut(s) | 211, 217, 223, 521 |
| HpyCH4III | ACNGT | 2 cut(s) | 25, 208 |
| HpyCH4IV | ACGT | 1 cut(s) | 314 |
| HpyCH4V | TGCA | 1 cut(s) | 505 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 120, 401 |
| HpySE526I | ACGT | 1 cut(s) | 314 |
| Hsp92I | GRCGYC | 1 cut(s) | 45 |
| Hsp92II | CATG | 1 cut(s) | 509 |
| HspAI | GCGC | 3 cut(s) | 147, 149, 404 |
| KspI | CCGCGG | 1 cut(s) | 239 |
| Kzo9I | GATC | 1 cut(s) | 12 |
| LmnI | GCTCC | 1 cut(s) | 232 |
| LpnPI | CCDG | 7 cut(s) | 121, 154, 178, 205, 420, 456, 545 |
| MaeI | CTAG | 1 cut(s) | 104 |
| MaeII | ACGT | 1 cut(s) | 314 |
| MaeIII | GTNAC | 2 cut(s) | 258, 344 |
| MalI | GATC | 1 cut(s) | 14 |
| MboI | GATC | 1 cut(s) | 12 |
| MboII | GAAGA | 3 cut(s) | 140, 214, 226 |
| MhlI | GDGCHC | 1 cut(s) | 442 |
| MluCI | AATT | 1 cut(s) | 253 |
| MseI | TTAA | 2 cut(s) | 327, 477 |
| MslI | CAYNNNNRTG | 1 cut(s) | 510 |
| MspA1I | CMGCKG | 2 cut(s) | 238, 269 |
| MspI | CCGG | 1 cut(s) | 407 |
| MspR9I | CCNGG | 2 cut(s) | 193, 560 |
| MvaI | CCWGG | 2 cut(s) | 193, 560 |
| MvnI | CGCG | 3 cut(s) | 147, 149, 238 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 120, 401 |
| NdeII | GATC | 1 cut(s) | 12 |
| NlaIII | CATG | 1 cut(s) | 509 |
| NlaIV | GGNNCC | 1 cut(s) | 234 |
| NmeAIII | GCCGAG | 2 cut(s) | 421, 474 |
| NmuCI | GTSAC | 2 cut(s) | 258, 344 |
| NspI | RCATGY | 1 cut(s) | 509 |
| PauI | GCGCGC | 1 cut(s) | 147 |
| PfeI | GAWTC | 1 cut(s) | 170 |
| PflFI | GACNNNGTC | 1 cut(s) | 23 |
| PkrI | GCNGC | 6 cut(s) | 146, 237, 240, 271, 274, 447 |
| Psp6I | CCWGG | 2 cut(s) | 191, 558 |
| PspGI | CCWGG | 2 cut(s) | 191, 558 |
| PspN4I | GGNNCC | 1 cut(s) | 234 |
| PspPI | GGNCC | 3 cut(s) | 138, 189, 240 |
| PsyI | GACNNNGTC | 1 cut(s) | 23 |
| PteI | GCGCGC | 1 cut(s) | 147 |
| RsaI | GTAC | 1 cut(s) | 41 |
| RsaNI | GTAC | 1 cut(s) | 40 |
| RseI | CAYNNNNRTG | 1 cut(s) | 510 |
| SacII | CCGCGG | 1 cut(s) | 239 |
| SaqAI | TTAA | 2 cut(s) | 327, 477 |
| SatI | GCNGC | 6 cut(s) | 145, 236, 239, 270, 273, 446 |
| Sau3AI | GATC | 1 cut(s) | 12 |
| Sau96I | GGNCC | 3 cut(s) | 138, 189, 240 |
| ScrFI | CCNGG | 2 cut(s) | 193, 560 |
| SduI | GDGCHC | 1 cut(s) | 442 |
| SetI | ASST | 2 cut(s) | 317, 561 |
| Sfr303I | CCGCGG | 1 cut(s) | 239 |
| SgrBI | CCGCGG | 1 cut(s) | 239 |
| SinI | GGWCC | 1 cut(s) | 138 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 510 |
| Sse9I | AATT | 1 cut(s) | 253 |
| SsiI | CCGC | 9 cut(s) | 145, 236, 238, 269, 272, 275, 430, 446, 500 |
| SspMI | CTAG | 1 cut(s) | 104 |
| StyD4I | CCNGG | 2 cut(s) | 191, 558 |
| StyI | CCWWGG | 1 cut(s) | 103 |
| TaaI | ACNGT | 2 cut(s) | 25, 208 |
| TaiI | ACGT | 1 cut(s) | 317 |
| TaqI | TCGA | 1 cut(s) | 510 |
| TasI | AATT | 1 cut(s) | 253 |
| TauI | GCSGC | 6 cut(s) | 147, 238, 241, 272, 275, 448 |
| TfiI | GAWTC | 1 cut(s) | 170 |
| Tru1I | TTAA | 2 cut(s) | 327, 477 |
| Tru9I | TTAA | 2 cut(s) | 327, 477 |
| TseFI | GTSAC | 2 cut(s) | 258, 344 |
| Tsp45I | GTSAC | 2 cut(s) | 258, 344 |
| TspDTI | ATGAA | 2 cut(s) | 183, 291 |
| Tth111I | GACNNNGTC | 1 cut(s) | 23 |
| VpaK11BI | GGWCC | 1 cut(s) | 138 |
| XceI | RCATGY | 1 cut(s) | 509 |
| XmaJI | CCTAGG | 1 cut(s) | 103 |
| XmiI | GTMKAC | 1 cut(s) | 27 |
| XspI | CTAG | 1 cut(s) | 104 |