Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0000310

Physical Location & Seq

Genomic Coordinates Forward (+)

15327905 .. 15330328

Gene Structure 2424 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1174400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1023 bp

ATGAGAAACGGACAAGTAGCACTTGAGAAATATGTCATCACTAAGTCATTGACTAAACCACCTGAAGCATACCCAGATGCCAAAAACCAACCACATGTTCAGGTGGCATTAAGATTAAAGCAAAGTGGTTATTCTGCTGGTTGTTCTGCTGGCGATACAGTCCCCTATATCATTTGCTGTGAGCAGGGGACTAGTTTAGGTAATTCAACGGTGATTGCTCAACGTGCTAGACACCCCGATGAGTTAAAACGAGAAGATGGGAAATGGATGATTGACATTGATTACTACTTGTCCCAGCAGATTCATCCTGTGGTATCACGTCTTTGTGCTTCAATTCAGGGTACCAGCCCCGGACGCTTGGCTGATTGTTTAGGACTTGACTCTTCTAAGGTGACTCAAGTGAAGCTATTTCGCGGTTGTGAGCCTTTGATCTTGGCATGTCCTAGCTGTTCCGATACTTTTAGTTGTCCAACTATCTTGAATTCCATTTCTATGTCAATCACCAAAAAGCAAACAAAGTCACAAGCCGAGGAGTGTACCAGCGATTTTTGGTATACATTACGATGTGCAAAATGCCCCGGGGAAGGAGACATGGGCAGACTGTCTCCTGTAATGATAGCCAACCAGGTGAAAAGGCAAGTAGACGGTTTTGTTTCAATGTACTATAGGGGCTTTATGACGTGTGACGATGAATCTTGCCAGTACAACACACGAAGTCTCAACCTCCGGCTTGTTGGCAATGCTGAGAGGGGAACAGTTTGTCCAGACTACCCTCGCTGCAATGGGCGTCTTGTAAGAAAGTACACGGAAGCAGATGTGTACAAGCAGCTCTCGTTTTTCTGCCATGTCTTGGATACAGTGCGCTGCATAGAAAAGACGGAGGCAAGCACTAGACTACCGGTAGAGATGGAGTTGGCCAAACTTCGGTCAGTTGTGAATTTGGCTGCCTCAACAGTTCAGAGGATTCGGGACCGTTGTGCGTACGGATGGATACAGCTGCAAGATCTTTGCATTACAGTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000228 GO:0000428 GO:0000726 GO:0003674 GO:0003824 GO:0003887 GO:0003896 GO:0003899 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005657 GO:0005658 GO:0005694 GO:0006139 GO:0006259 GO:0006260 GO:0006261 GO:0006269 GO:0006270 GO:0006271 GO:0006272 GO:0006273 GO:0006281 GO:0006302 GO:0006303 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009653 GO:0009965 GO:0009987 GO:0010016 GO:0016070 GO:0016740 GO:0016772 GO:0016779 GO:0018130 GO:0019438 GO:0022616 GO:0030880 GO:0030894 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032774 GO:0032991 GO:0032993 GO:0033554 GO:0034061 GO:0034062 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043596 GO:0043601 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044446 GO:0044454 GO:0044464 GO:0046483 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0050896 GO:0051716 GO:0055029 GO:0061695 GO:0070013 GO:0071704 GO:0071897 GO:0090304 GO:0097747 GO:0099402 GO:0140097 GO:0140098 GO:1901360 GO:1901362 GO:1901576 GO:1902494 GO:1905392 GO:1990234

KEGG Pathways

Metabolic & Signaling

ko00230 ko00240 ko01100 ko03030 map00230 map00240 map01100 map03030

Pfam Domains

Protein Families

DNA_pol_B DNA_pol_B_exo1 DNA_pol_alpha_N zf-DNA_Pol

Protein Analysis

341

Amino Acids

37.97

Weight (kDa)

8.52

Isoelectric Point (pI)

43.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DNA_pol_B	PF00136	1 - 112	6e-20	DNA polymerase family B
zf-DNA_Pol	PF08996	130 - 336	4.8e-38	DNA Polymerase alpha zinc finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0000310)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G67100 AT5G67100
fragaria_vesca	FvH4_1g20001 FvH4_1g22730 FvH4_1g22730 FvH4_1g22730 FvH4_6g13861 FvH4_6g33603
malus_domestica	MD02G1174100.v1.1 MD02G1174200.v1.1 MD02G1174400.v1.1 MD15G1285400.v1.1
prunus_persica	Prupe.7G131600_v2.0.a1
pyrus_communis	pycom02g14040 pycom02g14050 pycom02g14060 pycom02g14070 pycom15g24830 pycom15g24840
rosa_chinensis	RchiOBHm_Chr2g0109901 RchiOBHm_Chr2g0117021 RchiOBHm_Chr2g0168121 RchiOBHm_Chr4g0394581
rosa_laevigata	RLG00000001621 RLG00000001943 RLG00000003450 RLG00000006116 RLG00000006535 RLG00000008147 RLG00000009121 RLG00000010680 RLG00000013987 RLG00000017585 RLG00000017603 RLG00000017638 RLG00000017923 RLG00000018320 RLG00000021520 RLG00000022613 RLG00000022614 RLG00000023966 RLG00000025998 RLG00000027760 RLG00000033421
rosa_multiflora	Rmu_co7985806.1_g000001 Rmu_co8250293.1_g000001 Rmu_co8353517.1_g000001 Rmu_sc0000697.1_g000011 Rmu_sc0000776.1_g000079 Rmu_sc0001023.1_g000057 Rmu_sc0001266.1_g000019 Rmu_sc0002711.1_g000010 Rmu_sc0002731.1_g000005 Rmu_sc0003002.1_g000047 Rmu_sc0003030.1_g000002 Rmu_sc0003672.1_g000003 Rmu_sc0004168.1_g000062 Rmu_sc0004180.1_g000003 Rmu_sc0009205.1_g000008 Rmu_sc0011259.1_g000001 Rmu_sc0011259.1_g000002 Rmu_sc0027274.1_g000001
rosa_roxburghii	Rroxscaffold_1G00023520 Rroxscaffold_2G00104450 Rroxscaffold_2G00126810 Rroxscaffold_2G00144870 Rroxscaffold_4G00315680 Rroxscaffold_4G00331480 Rroxscaffold_7G00182820
rosa_rugosa	Rorug02G0209600 Rorug04G0130600 Rorug04G0130600 Rorug04G0130700 Rorug05G0230000
rosa_samantha	Rh1AG170000 Rh1CG157700 Rh1CG385600 Rh2AG215100 Rh2AG265500 Rh2BG277300 Rh2CG217600 Rh2CG217700 Rh2DG220600 Rh2DG272800 Rh2DG291800 Rh3AG215700 Rh3BG271700 Rh3DG075200 Rh3DG242600 Rh4AG115400 Rh4AG138100 Rh4AG333600 Rh4BG115100 Rh4BG366300 Rh4CG125400 Rh4CG380400 Rh4DG083500 Rh4DG113200 Rh4DG192900 Rh4DG246300 Rh5AG075000 Rh5BG482300 Rh5CG181200 Rh5CG247200 Rh5CG506900 Rh5DG167600 Rh5DG202900 Rh6AG000500 Rh6AG213400 Rh6BG335800 Rh6CG255700 Rh7AG337500 Rh7BG404500 Rh7BG461500 Rh7CG355600
rosa_wichuraiana	Rw0G019110 Rw2G020950

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	341
AccB1I	GGYRCC	1 cut(s)	341
AccB7I	CCANNNNNTGG	1 cut(s)	850
AccI	GTMKAC	2 cut(s)	554, 642
AccII	CGCG	1 cut(s)	414
AciI	CCGC	1 cut(s)	414
AcoI	YGGCCR	1 cut(s)	915
AcsI	RAATTY	2 cut(s)	481, 937
AcuI	CTGAAG	1 cut(s)	84
AcyI	GRCGYC	1 cut(s)	787
AfaI	GTAC	7 cut(s)	343, 538, 662, 704, 803, 821, 983
AfiI	CCNNNNNNNGG	3 cut(s)	584, 850, 924
AflIII	ACRYGT	2 cut(s)	94, 680
AgeI	ACCGGT	1 cut(s)	898
AgsI	TTSAA	4 cut(s)	207, 333, 481, 657
AhlI	ACTAGT	1 cut(s)	191
AjiI	CACGTC	2 cut(s)	320, 681
AjnI	CCWGG	1 cut(s)	624
AjuI	GAANNNNNNNTTGG	2 cut(s)	81, 113
AloI	GAACNNNNNNTCC	2 cut(s)	745, 777
AluBI	AGCT	4 cut(s)	406, 447, 829, 997
AluI	AGCT	4 cut(s)	406, 447, 829, 997
Alw26I	GTCTC	3 cut(s)	582, 609, 722
Ama87I	CYCGRG	1 cut(s)	578
AoxI	GGCC	1 cut(s)	915
ApeKI	GCWGC	5 cut(s)	777, 826, 864, 944, 997
ApoI	RAATTY	2 cut(s)	481, 937
AsiGI	ACCGGT	1 cut(s)	898
Asp718I	GGTACC	1 cut(s)	341
AspLEI	GCGC	1 cut(s)	864
AspS9I	GGNCC	1 cut(s)	970
AsuC2I	CCSGG	3 cut(s)	351, 579, 580
AsuHPI	GGTGA	4 cut(s)	223, 403, 493, 640
AvaI	CYCGRG	1 cut(s)	578
AvaII	GGWCC	1 cut(s)	970
BalI	TGGCCA	1 cut(s)	917
BanI	GGYRCC	1 cut(s)	341
BbvI	GCAGC	5 cut(s)	764, 838, 851, 931, 984
BccI	CCATC	3 cut(s)	251, 901, 981
BciT130I	CCWGG	1 cut(s)	626
BciVI	GTATCC	2 cut(s)	847, 984
BcnI	CCSGG	3 cut(s)	351, 579, 580
BcoDI	GTCTC	3 cut(s)	582, 609, 722
BcuI	ACTAGT	1 cut(s)	191
BfaI	CTAG	4 cut(s)	192, 228, 444, 891
BfmI	CTRYAG	1 cut(s)	664
BfuI	GTATCC	2 cut(s)	847, 984
BglII	AGATCT	1 cut(s)	1003
BisI	GCNGC	5 cut(s)	778, 827, 865, 945, 998
BlsI	GCNGC	5 cut(s)	779, 828, 866, 946, 999
Bme1390I	CCNGG	4 cut(s)	351, 579, 580, 626
Bme18I	GGWCC	1 cut(s)	970
BmeT110I	CYCGRG	1 cut(s)	578
BmgBI	CACGTC	2 cut(s)	320, 681
BmgT120I	GGNCC	1 cut(s)	970
BmiI	GGNNCC	2 cut(s)	343, 971
BmrFI	CCNGG	4 cut(s)	351, 579, 580, 626
BmsI	GCATC	1 cut(s)	67
BpuEI	CTTGAG	2 cut(s)	44, 381
BpuMI	CCSGG	3 cut(s)	351, 579, 580
BsaHI	GRCGYC	1 cut(s)	787
BsaJI	CCNNGG	5 cut(s)	349, 528, 577, 578, 579
BsaWI	WCCGGW	1 cut(s)	898
Bsc4I	CCNNNNNNNGG	3 cut(s)	584, 850, 924
Bse118I	RCCGGY	1 cut(s)	898
Bse1I	ACTGG	1 cut(s)	700
Bse3DI	GCAATG	2 cut(s)	745, 787
BseBI	CCWGG	1 cut(s)	626
BseDI	CCNNGG	5 cut(s)	349, 528, 577, 578, 579
BseGI	GGATG	3 cut(s)	273, 304, 992
BseLI	CCNNNNNNNGG	3 cut(s)	584, 850, 924
BseMI	GCAATG	2 cut(s)	745, 787
BseMII	CTCAG	1 cut(s)	735
BseNI	ACTGG	1 cut(s)	700
BseRI	GAGGAG	1 cut(s)	545
BseXI	GCAGC	5 cut(s)	764, 838, 851, 931, 984
BseYI	CCCAGC	1 cut(s)	294
Bsh1236I	CGCG	1 cut(s)	414
BshFI	GGCC	1 cut(s)	917
BshNI	GGYRCC	1 cut(s)	341
BshTI	ACCGGT	1 cut(s)	898
BsiHKCI	CYCGRG	1 cut(s)	578
BsiSI	CCGG	4 cut(s)	351, 579, 727, 899
BsiWI	CGTACG	1 cut(s)	981
BslFI	GGGAC	4 cut(s)	146, 202, 277, 983
BslI	CCNNNNNNNGG	3 cut(s)	584, 850, 924
BsmAI	GTCTC	3 cut(s)	582, 609, 722
BsmFI	GGGAC	4 cut(s)	146, 202, 277, 983
BsnI	GGCC	1 cut(s)	917
BsoBI	CYCGRG	1 cut(s)	578
Bsp1407I	TGTACA	1 cut(s)	819
Bsp143I	GATC	2 cut(s)	429, 1003
BspACI	CCGC	1 cut(s)	414
BspANI	GGCC	1 cut(s)	917
BspCNI	CTCAG	1 cut(s)	736
BspFNI	CGCG	1 cut(s)	414
BspLI	GGNNCC	2 cut(s)	343, 971
BspT107I	GGYRCC	1 cut(s)	341
BsrDI	GCAATG	2 cut(s)	745, 787
BsrFI	RCCGGY	1 cut(s)	898
BsrGI	TGTACA	1 cut(s)	819
BsrI	ACTGG	1 cut(s)	700
BssAI	RCCGGY	1 cut(s)	898
BssECI	CCNNGG	5 cut(s)	349, 528, 577, 578, 579
BssMI	GATC	2 cut(s)	429, 1003
BssNAI	GTATAC	1 cut(s)	555
BssNI	GRCGYC	1 cut(s)	787
Bst1107I	GTATAC	1 cut(s)	555
Bst2UI	CCWGG	1 cut(s)	626
Bst4CI	ACNGT	9 cut(s)	160, 211, 603, 647, 757, 859, 955, 974, 1018
Bst6I	CTCTTC	1 cut(s)	388
BstACI	GRCGYC	1 cut(s)	787
BstAUI	TGTACA	1 cut(s)	819
BstC8I	GCNNGC	2 cut(s)	151, 886
BstDEI	CTNAG	3 cut(s)	42, 387, 744
BstF5I	GGATG	3 cut(s)	273, 304, 992
BstFNI	CGCG	1 cut(s)	414
BstHHI	GCGC	1 cut(s)	864
BstKTI	GATC	2 cut(s)	432, 1006
BstMAI	GTCTC	3 cut(s)	582, 609, 722
BstMBI	GATC	2 cut(s)	429, 1003
BstMWI	GCNNNNNNNGC	2 cut(s)	224, 354
BstNI	CCWGG	1 cut(s)	626
BstNSI	RCATGY	2 cut(s)	98, 441
BstSCI	CCNGG	4 cut(s)	349, 577, 578, 624
BstSFI	CTRYAG	1 cut(s)	664
BstUI	CGCG	1 cut(s)	414
BstV1I	GCAGC	5 cut(s)	764, 838, 851, 931, 984
BstX2I	RGATCY	1 cut(s)	1003
BstYI	RGATCY	1 cut(s)	1003
BstZ17I	GTATAC	1 cut(s)	555
BsuI	GTATCC	2 cut(s)	847, 984
BsuRI	GGCC	1 cut(s)	917
BtrI	CACGTC	2 cut(s)	320, 681
BtsCI	GGATG	3 cut(s)	273, 304, 992
BtsIMutI	CAGTG	1 cut(s)	864
Cac8I	GCNNGC	2 cut(s)	151, 886
CfoI	GCGC	1 cut(s)	864
Cfr10I	RCCGGY	1 cut(s)	898
Cfr13I	GGNCC	1 cut(s)	970
Cfr9I	CCCGGG	1 cut(s)	578
CseI	GACGC	2 cut(s)	363, 776
CsiI	ACCWGGT	1 cut(s)	624
Csp6I	GTAC	7 cut(s)	342, 537, 661, 703, 802, 820, 982
CspAI	ACCGGT	1 cut(s)	898
CviAII	CATG	4 cut(s)	95, 438, 592, 845
CviQI	GTAC	7 cut(s)	342, 537, 661, 703, 802, 820, 982
DdeI	CTNAG	3 cut(s)	42, 387, 744
DpnI	GATC	2 cut(s)	431, 1005
DpnII	GATC	2 cut(s)	429, 1003
EaeI	YGGCCR	1 cut(s)	915
Eam1104I	CTCTTC	1 cut(s)	388
EarI	CTCTTC	1 cut(s)	388
Eco47I	GGWCC	1 cut(s)	970
Eco57I	CTGAAG	1 cut(s)	84
Eco88I	CYCGRG	1 cut(s)	578
EcoRI	GAATTC	1 cut(s)	481
EcoRII	CCWGG	1 cut(s)	624
FaeI	CATG	4 cut(s)	98, 441, 595, 848
FalI	AAGNNNNNCTT	1 cut(s)	38
FaqI	GGGAC	4 cut(s)	146, 202, 277, 983
FatI	CATG	4 cut(s)	94, 437, 591, 844
FblI	GTMKAC	2 cut(s)	554, 642
Fnu4HI	GCNGC	5 cut(s)	778, 827, 865, 945, 998
FokI	GGATG	3 cut(s)	280, 291, 999
Fsp4HI	GCNGC	5 cut(s)	778, 827, 865, 945, 998
FspBI	CTAG	4 cut(s)	192, 228, 444, 891
GlaI	GCGC	1 cut(s)	863
GluI	GCNGC	5 cut(s)	778, 827, 865, 945, 998
GsaI	CCCAGC	1 cut(s)	298
HaeIII	GGCC	1 cut(s)	917
HapII	CCGG	4 cut(s)	351, 579, 727, 899
HgaI	GACGC	2 cut(s)	363, 776
HhaI	GCGC	1 cut(s)	864
Hin1I	GRCGYC	1 cut(s)	787
Hin1II	CATG	4 cut(s)	98, 441, 595, 848
Hin6I	GCGC	1 cut(s)	862
HinP1I	GCGC	1 cut(s)	862
HinfI	GANTC	5 cut(s)	301, 380, 394, 692, 964
HpaII	CCGG	4 cut(s)	351, 579, 727, 899
HphI	GGTGA	4 cut(s)	223, 403, 493, 640
Hpy166II	GTNNAC	5 cut(s)	537, 555, 643, 804, 820
Hpy188I	TCNGA	2 cut(s)	454, 960
Hpy188III	TCNNGA	3 cut(s)	478, 764, 968
Hpy8I	GTNNAC	5 cut(s)	537, 555, 643, 804, 820
HpyAV	CCTTC	1 cut(s)	578
HpyCH4III	ACNGT	9 cut(s)	160, 211, 603, 647, 757, 859, 955, 974, 1018
HpyCH4IV	ACGT	3 cut(s)	223, 319, 680
HpyCH4V	TGCA	5 cut(s)	569, 780, 867, 1000, 1011
HpyF10VI	GCNNNNNNNGC	2 cut(s)	224, 354
HpyF3I	CTNAG	3 cut(s)	42, 387, 744
HpySE526I	ACGT	3 cut(s)	223, 319, 680
Hsp92I	GRCGYC	1 cut(s)	787
Hsp92II	CATG	4 cut(s)	98, 441, 595, 848
HspAI	GCGC	1 cut(s)	862
KpnI	GGTACC	1 cut(s)	345
Kzo9I	GATC	2 cut(s)	429, 1003
Lsp1109I	GCAGC	5 cut(s)	764, 838, 851, 931, 984
LweI	GCATC	1 cut(s)	67
MabI	ACCWGGT	1 cut(s)	624
MaeI	CTAG	4 cut(s)	192, 228, 444, 891
MaeII	ACGT	3 cut(s)	223, 319, 680
MaeIII	GTNAC	3 cut(s)	391, 519, 683
MalI	GATC	2 cut(s)	431, 1005
MboI	GATC	2 cut(s)	429, 1003
MboII	GAAGA	2 cut(s)	266, 375
MflI	RGATCY	1 cut(s)	1003
MlsI	TGGCCA	1 cut(s)	917
MluCI	AATT	4 cut(s)	202, 333, 481, 937
MluNI	TGGCCA	1 cut(s)	917
MlyI	GAGTC	2 cut(s)	374, 388
MmeI	TCCRAC	1 cut(s)	494
MnlI	CCTC	7 cut(s)	523, 734, 741, 783, 874, 954, 958
Mox20I	TGGCCA	1 cut(s)	917
MscI	TGGCCA	1 cut(s)	917
MseI	TTAA	4 cut(s)	110, 116, 245, 1021
MslI	CAYNNNNRTG	3 cut(s)	237, 491, 562
Msp20I	TGGCCA	1 cut(s)	917
MspA1I	CMGCKG	1 cut(s)	997
MspI	CCGG	4 cut(s)	351, 579, 727, 899
MspR9I	CCNGG	4 cut(s)	351, 579, 580, 626
MvaI	CCWGG	1 cut(s)	626
MvnI	CGCG	1 cut(s)	414
MwoI	GCNNNNNNNGC	2 cut(s)	224, 354
NciI	CCSGG	3 cut(s)	351, 579, 580
NdeII	GATC	2 cut(s)	429, 1003
NlaIII	CATG	4 cut(s)	98, 441, 595, 848
NlaIV	GGNNCC	2 cut(s)	343, 971
NmeAIII	GCCGAG	1 cut(s)	553
NmuCI	GTSAC	3 cut(s)	391, 519, 683
NspI	RCATGY	2 cut(s)	98, 441
PciI	ACATGT	1 cut(s)	94
PfeI	GAWTC	3 cut(s)	301, 692, 964
Pfl23II	CGTACG	1 cut(s)	981
PflMI	CCANNNNNTGG	1 cut(s)	850
PinAI	ACCGGT	1 cut(s)	898
PkrI	GCNGC	5 cut(s)	779, 828, 866, 946, 999
PleI	GAGTC	2 cut(s)	374, 388
PpsI	GAGTC	2 cut(s)	374, 388
PscI	ACATGT	1 cut(s)	94
Psp6I	CCWGG	1 cut(s)	624
PspFI	CCCAGC	1 cut(s)	294
PspGI	CCWGG	1 cut(s)	624
PspLI	CGTACG	1 cut(s)	981
PspN4I	GGNNCC	2 cut(s)	343, 971
PspPI	GGNCC	1 cut(s)	970
PsuI	RGATCY	1 cut(s)	1003
PvuII	CAGCTG	1 cut(s)	997
RsaI	GTAC	7 cut(s)	343, 538, 662, 704, 803, 821, 983
RsaNI	GTAC	7 cut(s)	342, 537, 661, 703, 802, 820, 982
RseI	CAYNNNNRTG	3 cut(s)	237, 491, 562
SaqAI	TTAA	4 cut(s)	110, 116, 245, 1021
SatI	GCNGC	5 cut(s)	778, 827, 865, 945, 998
Sau3AI	GATC	2 cut(s)	429, 1003
Sau96I	GGNCC	1 cut(s)	970
SchI	GAGTC	2 cut(s)	374, 388
ScrFI	CCNGG	4 cut(s)	351, 579, 580, 626
SexAI	ACCWGGT	1 cut(s)	624
SfaNI	GCATC	1 cut(s)	67
SfcI	CTRYAG	1 cut(s)	664
SinI	GGWCC	1 cut(s)	970
SmaI	CCCGGG	1 cut(s)	580
SmiMI	CAYNNNNRTG	3 cut(s)	237, 491, 562
SmlI	CTYRAG	2 cut(s)	23, 396
SmoI	CTYRAG	2 cut(s)	23, 396
SpeI	ACTAGT	1 cut(s)	191
Sse9I	AATT	4 cut(s)	202, 333, 481, 937
SsiI	CCGC	1 cut(s)	414
SspMI	CTAG	4 cut(s)	192, 228, 444, 891
StyD4I	CCNGG	4 cut(s)	349, 577, 578, 624
TaaI	ACNGT	9 cut(s)	160, 211, 603, 647, 757, 859, 955, 974, 1018
TaiI	ACGT	3 cut(s)	226, 322, 683
TaqII	GACCGA	1 cut(s)	915
TasI	AATT	4 cut(s)	202, 333, 481, 937
TatI	WGTACW	4 cut(s)	660, 702, 801, 819
TfiI	GAWTC	3 cut(s)	301, 692, 964
Tru1I	TTAA	4 cut(s)	110, 116, 245, 1021
Tru9I	TTAA	4 cut(s)	110, 116, 245, 1021
TscAI	CASTG	1 cut(s)	864
TseFI	GTSAC	3 cut(s)	391, 519, 683
TseI	GCWGC	5 cut(s)	777, 826, 864, 944, 997
Tsp45I	GTSAC	3 cut(s)	391, 519, 683
TspDTI	ATGAA	2 cut(s)	293, 705
TspGWI	ACGGA	4 cut(s)	24, 821, 893, 999
TspMI	CCCGGG	1 cut(s)	578
TspRI	CASTG	1 cut(s)	864
Van91I	CCANNNNNTGG	1 cut(s)	850
VpaK11BI	GGWCC	1 cut(s)	970
XapI	RAATTY	2 cut(s)	481, 937
XceI	RCATGY	2 cut(s)	98, 441
XmaI	CCCGGG	1 cut(s)	578
XmiI	GTMKAC	2 cut(s)	554, 642
XspI	CTAG	4 cut(s)	192, 228, 444, 891

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.