DNA polymerase
| Domain Name | Pfam ID | Position | E-value | Description |
|---|---|---|---|---|
| DNA_pol_alpha_N | PF12254 | 2 - 35 | 2.6e-07 | DNA polymerase alpha subunit p180 N terminal |
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 140 |
| Acc65I | GGTACC | 1 cut(s) | 494 |
| AccB1I | GGYRCC | 1 cut(s) | 494 |
| AccI | GTMKAC | 1 cut(s) | 27 |
| AccII | CGCG | 1 cut(s) | 126 |
| AciI | CCGC | 3 cut(s) | 61, 126, 254 |
| AclWI | GGATC | 2 cut(s) | 490, 546 |
| AcsI | RAATTY | 1 cut(s) | 680 |
| AcuI | CTGAAG | 1 cut(s) | 555 |
| AcyI | GRCGYC | 2 cut(s) | 45, 137 |
| AfaI | GTAC | 2 cut(s) | 41, 496 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 126, 177 |
| AjiI | CACGTC | 1 cut(s) | 294 |
| AjnI | CCWGG | 1 cut(s) | 170 |
| AluBI | AGCT | 2 cut(s) | 630, 643 |
| AluI | AGCT | 2 cut(s) | 630, 643 |
| Alw21I | GWGCWC | 1 cut(s) | 632 |
| Alw26I | GTCTC | 2 cut(s) | 400, 435 |
| AlwI | GGATC | 2 cut(s) | 490, 546 |
| AoxI | GGCC | 3 cut(s) | 167, 218, 330 |
| ApeKI | GCWGC | 2 cut(s) | 635, 657 |
| ApoI | RAATTY | 1 cut(s) | 680 |
| Asp718I | GGTACC | 1 cut(s) | 494 |
| AspLEI | GCGC | 1 cut(s) | 385 |
| AspS9I | GGNCC | 3 cut(s) | 117, 168, 219 |
| AsuHPI | GGTGA | 1 cut(s) | 335 |
| AvaII | GGWCC | 1 cut(s) | 117 |
| BaeGI | GKGCMC | 1 cut(s) | 421 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 558, 591 |
| BanI | GGYRCC | 1 cut(s) | 494 |
| BanII | GRGCYC | 2 cut(s) | 588, 632 |
| BbsI | GAAGAC | 1 cut(s) | 188 |
| Bbv12I | GWGCWC | 1 cut(s) | 632 |
| BbvI | GCAGC | 2 cut(s) | 622, 644 |
| BccI | CCATC | 4 cut(s) | 56, 256, 314, 593 |
| BceAI | ACGGC | 3 cut(s) | 172, 233, 261 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 506, 540 |
| BciT130I | CCWGG | 1 cut(s) | 172 |
| BciVI | GTATCC | 1 cut(s) | 559 |
| BcoDI | GTCTC | 2 cut(s) | 400, 435 |
| BfuI | GTATCC | 1 cut(s) | 559 |
| BglII | AGATCT | 1 cut(s) | 695 |
| BisI | GCNGC | 3 cut(s) | 255, 636, 658 |
| BlsI | GCNGC | 3 cut(s) | 256, 637, 659 |
| Bme1390I | CCNGG | 1 cut(s) | 172 |
| Bme18I | GGWCC | 1 cut(s) | 117 |
| BmgBI | CACGTC | 1 cut(s) | 294 |
| BmgT120I | GGNCC | 3 cut(s) | 117, 168, 219 |
| BmiI | GGNNCC | 3 cut(s) | 119, 213, 496 |
| BmrFI | CCNGG | 1 cut(s) | 172 |
| BpiI | GAAGAC | 1 cut(s) | 188 |
| BsaHI | GRCGYC | 2 cut(s) | 45, 137 |
| BsaJI | CCNNGG | 2 cut(s) | 170, 215 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 126, 177 |
| Bse118I | RCCGGY | 1 cut(s) | 424 |
| Bse1I | ACTGG | 1 cut(s) | 119 |
| BseBI | CCWGG | 1 cut(s) | 172 |
| BseDI | CCNNGG | 2 cut(s) | 170, 215 |
| BseGI | GGATG | 3 cut(s) | 325, 367, 541 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 126, 177 |
| BseMII | CTCAG | 1 cut(s) | 459 |
| BseNI | ACTGG | 1 cut(s) | 119 |
| BseRI | GAGGAG | 4 cut(s) | 83, 190, 427, 445 |
| BseSI | GKGCMC | 1 cut(s) | 421 |
| BseXI | GCAGC | 2 cut(s) | 622, 644 |
| Bsh1236I | CGCG | 1 cut(s) | 126 |
| BshFI | GGCC | 3 cut(s) | 169, 220, 332 |
| BshNI | GGYRCC | 1 cut(s) | 494 |
| BsiHKAI | GWGCWC | 1 cut(s) | 632 |
| BsiSI | CCGG | 2 cut(s) | 386, 425 |
| BslFI | GGGAC | 1 cut(s) | 92 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 126, 177 |
| BsmAI | GTCTC | 2 cut(s) | 400, 435 |
| BsmBI | CGTCTC | 1 cut(s) | 400 |
| BsmFI | GGGAC | 1 cut(s) | 92 |
| BsnI | GGCC | 3 cut(s) | 169, 220, 332 |
| Bsp1286I | GDGCHC | 3 cut(s) | 421, 588, 632 |
| Bsp143I | GATC | 4 cut(s) | 12, 482, 538, 695 |
| BspACI | CCGC | 3 cut(s) | 61, 126, 254 |
| BspANI | GGCC | 3 cut(s) | 169, 220, 332 |
| BspCNI | CTCAG | 1 cut(s) | 458 |
| BspFNI | CGCG | 1 cut(s) | 126 |
| BspLI | GGNNCC | 3 cut(s) | 119, 213, 496 |
| BspPI | GGATC | 2 cut(s) | 490, 546 |
| BspT107I | GGYRCC | 1 cut(s) | 494 |
| BsrFI | RCCGGY | 1 cut(s) | 424 |
| BsrI | ACTGG | 1 cut(s) | 119 |
| BssAI | RCCGGY | 1 cut(s) | 424 |
| BssECI | CCNNGG | 2 cut(s) | 170, 215 |
| BssMI | GATC | 4 cut(s) | 12, 482, 538, 695 |
| BssNI | GRCGYC | 2 cut(s) | 45, 137 |
| Bst2UI | CCWGG | 1 cut(s) | 172 |
| Bst4CI | ACNGT | 4 cut(s) | 25, 187, 494, 648 |
| Bst6I | CTCTTC | 1 cut(s) | 102 |
| BstACI | GRCGYC | 2 cut(s) | 45, 137 |
| BstC8I | GCNNGC | 2 cut(s) | 128, 426 |
| BstDEI | CTNAG | 1 cut(s) | 445 |
| BstDSI | CCRYGG | 1 cut(s) | 215 |
| BstF5I | GGATG | 3 cut(s) | 325, 367, 541 |
| BstFNI | CGCG | 1 cut(s) | 126 |
| BstHHI | GCGC | 1 cut(s) | 385 |
| BstKTI | GATC | 4 cut(s) | 15, 485, 541, 698 |
| BstMAI | GTCTC | 2 cut(s) | 400, 435 |
| BstMBI | GATC | 4 cut(s) | 12, 482, 538, 695 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 53, 226, 254, 380, 425 |
| BstNI | CCWGG | 1 cut(s) | 172 |
| BstSCI | CCNGG | 1 cut(s) | 170 |
| BstSLI | GKGCMC | 1 cut(s) | 421 |
| BstUI | CGCG | 1 cut(s) | 126 |
| BstV1I | GCAGC | 2 cut(s) | 622, 644 |
| BstV2I | GAAGAC | 1 cut(s) | 188 |
| BstX2I | RGATCY | 2 cut(s) | 482, 695 |
| BstYI | RGATCY | 2 cut(s) | 482, 695 |
| BsuI | GTATCC | 1 cut(s) | 559 |
| BsuRI | GGCC | 3 cut(s) | 169, 220, 332 |
| BtgI | CCRYGG | 1 cut(s) | 215 |
| BtgZI | GCGATG | 1 cut(s) | 37 |
| BtrI | CACGTC | 1 cut(s) | 294 |
| BtsCI | GGATG | 3 cut(s) | 325, 367, 541 |
| BtsI | GCAGTG | 2 cut(s) | 255, 522 |
| BtsIMutI | CAGTG | 2 cut(s) | 255, 522 |
| Cac8I | GCNNGC | 2 cut(s) | 128, 426 |
| CfoI | GCGC | 1 cut(s) | 385 |
| Cfr10I | RCCGGY | 1 cut(s) | 424 |
| Cfr13I | GGNCC | 3 cut(s) | 117, 168, 219 |
| CseI | GACGC | 2 cut(s) | 53, 132 |
| Csp6I | GTAC | 2 cut(s) | 40, 495 |
| CviQI | GTAC | 2 cut(s) | 40, 495 |
| DdeI | CTNAG | 1 cut(s) | 445 |
| DpnI | GATC | 4 cut(s) | 14, 484, 540, 697 |
| DpnII | GATC | 4 cut(s) | 12, 482, 538, 695 |
| Eam1104I | CTCTTC | 1 cut(s) | 102 |
| EarI | CTCTTC | 1 cut(s) | 102 |
| Ecl136II | GAGCTC | 1 cut(s) | 630 |
| Eco24I | GRGCYC | 2 cut(s) | 588, 632 |
| Eco47I | GGWCC | 1 cut(s) | 117 |
| Eco53kI | GAGCTC | 1 cut(s) | 630 |
| Eco57I | CTGAAG | 1 cut(s) | 555 |
| EcoICRI | GAGCTC | 1 cut(s) | 630 |
| EcoRI | GAATTC | 1 cut(s) | 680 |
| EcoRII | CCWGG | 1 cut(s) | 170 |
| EcoT38I | GRGCYC | 2 cut(s) | 588, 632 |
| Esp3I | CGTCTC | 1 cut(s) | 400 |
| FaqI | GGGAC | 1 cut(s) | 92 |
| FauI | CCCGC | 1 cut(s) | 119 |
| FauNDI | CATATG | 1 cut(s) | 580 |
| FblI | GTMKAC | 1 cut(s) | 27 |
| Fnu4HI | GCNGC | 3 cut(s) | 255, 636, 658 |
| FokI | GGATG | 3 cut(s) | 332, 374, 528 |
| FriOI | GRGCYC | 2 cut(s) | 588, 632 |
| Fsp4HI | GCNGC | 3 cut(s) | 255, 636, 658 |
| GlaI | GCGC | 1 cut(s) | 384 |
| GluI | GCNGC | 3 cut(s) | 255, 636, 658 |
| HaeIII | GGCC | 3 cut(s) | 169, 220, 332 |
| HapII | CCGG | 2 cut(s) | 386, 425 |
| HgaI | GACGC | 2 cut(s) | 53, 132 |
| HhaI | GCGC | 1 cut(s) | 385 |
| Hin1I | GRCGYC | 2 cut(s) | 45, 137 |
| Hin6I | GCGC | 1 cut(s) | 383 |
| HinP1I | GCGC | 1 cut(s) | 383 |
| HincII | GTYRAC | 1 cut(s) | 28 |
| HindII | GTYRAC | 1 cut(s) | 28 |
| HinfI | GANTC | 3 cut(s) | 149, 443, 518 |
| HpaII | CCGG | 2 cut(s) | 386, 425 |
| HphI | GGTGA | 1 cut(s) | 335 |
| Hpy166II | GTNNAC | 2 cut(s) | 28, 291 |
| Hpy188I | TCNGA | 4 cut(s) | 135, 154, 440, 565 |
| Hpy188III | TCNNGA | 2 cut(s) | 480, 699 |
| Hpy8I | GTNNAC | 2 cut(s) | 28, 291 |
| Hpy99I | CGWCG | 7 cut(s) | 32, 47, 98, 126, 139, 142, 298 |
| HpyAV | CCTTC | 3 cut(s) | 190, 196, 202 |
| HpyCH4III | ACNGT | 4 cut(s) | 25, 187, 494, 648 |
| HpyCH4IV | ACGT | 2 cut(s) | 137, 293 |
| HpyCH4V | TGCA | 3 cut(s) | 467, 638, 660 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 53, 226, 254, 380, 425 |
| HpyF3I | CTNAG | 1 cut(s) | 445 |
| HpySE526I | ACGT | 2 cut(s) | 137, 293 |
| Hsp92I | GRCGYC | 2 cut(s) | 45, 137 |
| HspAI | GCGC | 1 cut(s) | 383 |
| KpnI | GGTACC | 1 cut(s) | 498 |
| KroI | GCCGGC | 1 cut(s) | 424 |
| KroNI | GCCGGC | 1 cut(s) | 426 |
| Kzo9I | GATC | 4 cut(s) | 12, 482, 538, 695 |
| LmnI | GCTCC | 2 cut(s) | 63, 211 |
| LpnPI | CCDG | 7 cut(s) | 100, 157, 184, 399, 432, 438, 465 |
| Lsp1109I | GCAGC | 2 cut(s) | 622, 644 |
| MaeII | ACGT | 2 cut(s) | 137, 293 |
| MaeIII | GTNAC | 1 cut(s) | 323 |
| MalI | GATC | 4 cut(s) | 14, 484, 540, 697 |
| MboI | GATC | 4 cut(s) | 12, 482, 538, 695 |
| MboII | GAAGA | 5 cut(s) | 119, 193, 205, 228, 676 |
| MflI | RGATCY | 2 cut(s) | 482, 695 |
| MhlI | GDGCHC | 3 cut(s) | 421, 588, 632 |
| MluCI | AATT | 3 cut(s) | 232, 621, 680 |
| MlyI | GAGTC | 1 cut(s) | 437 |
| MmeI | TCCRAC | 1 cut(s) | 158 |
| MroNI | GCCGGC | 1 cut(s) | 424 |
| MseI | TTAA | 3 cut(s) | 306, 669, 692 |
| MspI | CCGG | 2 cut(s) | 386, 425 |
| MspR9I | CCNGG | 1 cut(s) | 172 |
| MvaI | CCWGG | 1 cut(s) | 172 |
| MvnI | CGCG | 1 cut(s) | 126 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 53, 226, 254, 380, 425 |
| NaeI | GCCGGC | 1 cut(s) | 426 |
| NdeI | CATATG | 1 cut(s) | 580 |
| NdeII | GATC | 4 cut(s) | 12, 482, 538, 695 |
| NgoMIV | GCCGGC | 1 cut(s) | 424 |
| NlaIV | GGNNCC | 3 cut(s) | 119, 213, 496 |
| NmeAIII | GCCGAG | 1 cut(s) | 400 |
| NmuCI | GTSAC | 1 cut(s) | 323 |
| PcsI | WCGNNNNNNNCGW | 3 cut(s) | 33, 93, 99 |
| PdiI | GCCGGC | 1 cut(s) | 426 |
| PfeI | GAWTC | 2 cut(s) | 149, 518 |
| PflFI | GACNNNGTC | 2 cut(s) | 23, 139 |
| PkrI | GCNGC | 3 cut(s) | 256, 637, 659 |
| PleI | GAGTC | 1 cut(s) | 437 |
| PpsI | GAGTC | 1 cut(s) | 437 |
| Psp124BI | GAGCTC | 1 cut(s) | 632 |
| Psp6I | CCWGG | 1 cut(s) | 170 |
| PspGI | CCWGG | 1 cut(s) | 170 |
| PspN4I | GGNNCC | 3 cut(s) | 119, 213, 496 |
| PspPI | GGNCC | 3 cut(s) | 117, 168, 219 |
| PsuI | RGATCY | 2 cut(s) | 482, 695 |
| PsyI | GACNNNGTC | 2 cut(s) | 23, 139 |
| RsaI | GTAC | 2 cut(s) | 41, 496 |
| RsaNI | GTAC | 2 cut(s) | 40, 495 |
| SacI | GAGCTC | 1 cut(s) | 632 |
| SalI | GTCGAC | 1 cut(s) | 26 |
| SaqAI | TTAA | 3 cut(s) | 306, 669, 692 |
| SatI | GCNGC | 3 cut(s) | 255, 636, 658 |
| Sau3AI | GATC | 4 cut(s) | 12, 482, 538, 695 |
| Sau96I | GGNCC | 3 cut(s) | 117, 168, 219 |
| SchI | GAGTC | 1 cut(s) | 437 |
| ScrFI | CCNGG | 1 cut(s) | 172 |
| SduI | GDGCHC | 3 cut(s) | 421, 588, 632 |
| SetI | ASST | 7 cut(s) | 140, 296, 451, 500, 550, 632, 645 |
| SinI | GGWCC | 1 cut(s) | 117 |
| Sse9I | AATT | 3 cut(s) | 232, 621, 680 |
| SsiI | CCGC | 3 cut(s) | 61, 126, 254 |
| SstI | GAGCTC | 1 cut(s) | 632 |
| StyD4I | CCNGG | 1 cut(s) | 170 |
| TaaI | ACNGT | 4 cut(s) | 25, 187, 494, 648 |
| TaiI | ACGT | 2 cut(s) | 140, 296 |
| TaqI | TCGA | 2 cut(s) | 27, 87 |
| TasI | AATT | 3 cut(s) | 232, 621, 680 |
| TauI | GCSGC | 1 cut(s) | 257 |
| TfiI | GAWTC | 2 cut(s) | 149, 518 |
| Tru1I | TTAA | 3 cut(s) | 306, 669, 692 |
| Tru9I | TTAA | 3 cut(s) | 306, 669, 692 |
| TscAI | CASTG | 2 cut(s) | 255, 529 |
| TseFI | GTSAC | 1 cut(s) | 323 |
| TseI | GCWGC | 2 cut(s) | 635, 657 |
| Tsp45I | GTSAC | 1 cut(s) | 323 |
| TspDTI | ATGAA | 5 cut(s) | 73, 162, 467, 567, 676 |
| TspGWI | ACGGA | 1 cut(s) | 424 |
| TspRI | CASTG | 2 cut(s) | 255, 529 |
| Tth111I | GACNNNGTC | 2 cut(s) | 23, 139 |
| VpaK11BI | GGWCC | 1 cut(s) | 117 |
| XapI | RAATTY | 1 cut(s) | 680 |
| XmiI | GTMKAC | 1 cut(s) | 27 |
| ZraI | GACGTC | 1 cut(s) | 138 |