DNA polymerase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 564 |
| Acc36I | ACCTGC | 1 cut(s) | 564 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 449 |
| AccII | CGCG | 1 cut(s) | 20 |
| AciI | CCGC | 3 cut(s) | 18, 44, 53 |
| AclWI | GGATC | 1 cut(s) | 556 |
| AcsI | RAATTY | 3 cut(s) | 272, 535, 609 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 173, 449 |
| AgsI | TTSAA | 2 cut(s) | 218, 373 |
| AluBI | AGCT | 3 cut(s) | 214, 264, 484 |
| AluI | AGCT | 3 cut(s) | 214, 264, 484 |
| Alw26I | GTCTC | 1 cut(s) | 316 |
| AlwI | GGATC | 1 cut(s) | 556 |
| AoxI | GGCC | 3 cut(s) | 15, 33, 440 |
| ApeKI | GCWGC | 2 cut(s) | 50, 524 |
| ApoI | RAATTY | 3 cut(s) | 272, 535, 609 |
| AspS9I | GGNCC | 2 cut(s) | 33, 164 |
| AsuC2I | CCSGG | 1 cut(s) | 37 |
| AsuHPI | GGTGA | 2 cut(s) | 237, 292 |
| AvaII | GGWCC | 1 cut(s) | 164 |
| BaeGI | GKGCMC | 1 cut(s) | 179 |
| BbsI | GAAGAC | 1 cut(s) | 204 |
| BbvI | GCAGC | 2 cut(s) | 62, 511 |
| BccI | CCATC | 3 cut(s) | 3, 110, 482 |
| BcnI | CCSGG | 1 cut(s) | 37 |
| BcoDI | GTCTC | 1 cut(s) | 316 |
| BfmI | CTRYAG | 2 cut(s) | 333, 525 |
| BfuAI | ACCTGC | 1 cut(s) | 564 |
| BisI | GCNGC | 4 cut(s) | 18, 51, 54, 525 |
| BlpI | GCTNAGC | 1 cut(s) | 458 |
| BlsI | GCNGC | 4 cut(s) | 19, 52, 55, 526 |
| Bme1390I | CCNGG | 1 cut(s) | 37 |
| Bme18I | GGWCC | 1 cut(s) | 164 |
| BmgT120I | GGNCC | 2 cut(s) | 33, 164 |
| BmiI | GGNNCC | 1 cut(s) | 34 |
| BmrFI | CCNGG | 1 cut(s) | 37 |
| BmsI | GCATC | 3 cut(s) | 133, 214, 428 |
| BpiI | GAAGAC | 1 cut(s) | 204 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 458 |
| BpuEI | CTTGAG | 3 cut(s) | 220, 330, 580 |
| BpuMI | CCSGG | 1 cut(s) | 37 |
| Bsa29I | ATCGAT | 1 cut(s) | 114 |
| BsaBI | GATNNNNATC | 2 cut(s) | 104, 110 |
| BsaI | GGTCTC | 1 cut(s) | 316 |
| BsaJI | CCNNGG | 1 cut(s) | 228 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 173, 449 |
| Bse1I | ACTGG | 1 cut(s) | 31 |
| Bse3DI | GCAATG | 1 cut(s) | 63 |
| Bse8I | GATNNNNATC | 2 cut(s) | 104, 110 |
| BseCI | ATCGAT | 1 cut(s) | 114 |
| BseDI | CCNNGG | 1 cut(s) | 228 |
| BseGI | GGATG | 2 cut(s) | 14, 229 |
| BseJI | GATNNNNATC | 2 cut(s) | 104, 110 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 173, 449 |
| BseMI | GCAATG | 1 cut(s) | 63 |
| BseMII | CTCAG | 1 cut(s) | 449 |
| BseNI | ACTGG | 1 cut(s) | 31 |
| BseSI | GKGCMC | 1 cut(s) | 179 |
| BseXI | GCAGC | 2 cut(s) | 62, 511 |
| Bsh1236I | CGCG | 1 cut(s) | 20 |
| BshFI | GGCC | 3 cut(s) | 17, 35, 442 |
| BshVI | ATCGAT | 1 cut(s) | 114 |
| BsiSI | CCGG | 2 cut(s) | 36, 546 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 173, 449 |
| BsmAI | GTCTC | 1 cut(s) | 316 |
| BsmI | GAATGC | 1 cut(s) | 295 |
| BsnI | GGCC | 3 cut(s) | 17, 35, 442 |
| Bso31I | GGTCTC | 1 cut(s) | 316 |
| Bsp1286I | GDGCHC | 1 cut(s) | 179 |
| Bsp143I | GATC | 3 cut(s) | 105, 282, 548 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 458 |
| BspACI | CCGC | 3 cut(s) | 18, 44, 53 |
| BspANI | GGCC | 3 cut(s) | 17, 35, 442 |
| BspCNI | CTCAG | 1 cut(s) | 450 |
| BspDI | ATCGAT | 1 cut(s) | 114 |
| BspFNI | CGCG | 1 cut(s) | 20 |
| BspLI | GGNNCC | 1 cut(s) | 34 |
| BspMAI | CTGCAG | 1 cut(s) | 529 |
| BspMI | ACCTGC | 1 cut(s) | 564 |
| BspPI | GGATC | 1 cut(s) | 556 |
| BspTNI | GGTCTC | 1 cut(s) | 316 |
| BsrDI | GCAATG | 1 cut(s) | 63 |
| BsrI | ACTGG | 1 cut(s) | 31 |
| BssECI | CCNNGG | 1 cut(s) | 228 |
| BssMI | GATC | 3 cut(s) | 105, 282, 548 |
| Bst4CI | ACNGT | 2 cut(s) | 334, 472 |
| Bst6I | CTCTTC | 1 cut(s) | 147 |
| BstC8I | GCNNGC | 1 cut(s) | 42 |
| BstDEI | CTNAG | 1 cut(s) | 458 |
| BstF5I | GGATG | 2 cut(s) | 14, 229 |
| BstFNI | CGCG | 1 cut(s) | 20 |
| BstKTI | GATC | 3 cut(s) | 108, 285, 551 |
| BstMAI | GTCTC | 1 cut(s) | 316 |
| BstMBI | GATC | 3 cut(s) | 105, 282, 548 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 41, 50, 570, 587 |
| BstNSI | RCATGY | 1 cut(s) | 139 |
| BstSCI | CCNGG | 1 cut(s) | 35 |
| BstSFI | CTRYAG | 2 cut(s) | 333, 525 |
| BstSLI | GKGCMC | 1 cut(s) | 179 |
| BstUI | CGCG | 1 cut(s) | 20 |
| BstV1I | GCAGC | 2 cut(s) | 62, 511 |
| BstV2I | GAAGAC | 1 cut(s) | 204 |
| Bsu15I | ATCGAT | 1 cut(s) | 114 |
| BsuRI | GGCC | 3 cut(s) | 17, 35, 442 |
| BsuTUI | ATCGAT | 1 cut(s) | 114 |
| BtgZI | GCGATG | 1 cut(s) | 35 |
| BtsCI | GGATG | 2 cut(s) | 14, 229 |
| BtsI | GCAGTG | 1 cut(s) | 122 |
| BtsIMutI | CAGTG | 1 cut(s) | 122 |
| BveI | ACCTGC | 1 cut(s) | 564 |
| Cac8I | GCNNGC | 1 cut(s) | 42 |
| Cfr13I | GGNCC | 2 cut(s) | 33, 164 |
| ClaI | ATCGAT | 1 cut(s) | 114 |
| CviAII | CATG | 1 cut(s) | 136 |
| DdeI | CTNAG | 1 cut(s) | 458 |
| DpnI | GATC | 3 cut(s) | 107, 284, 550 |
| DpnII | GATC | 3 cut(s) | 105, 282, 548 |
| Eam1104I | CTCTTC | 1 cut(s) | 147 |
| EarI | CTCTTC | 1 cut(s) | 147 |
| Eco31I | GGTCTC | 1 cut(s) | 316 |
| Eco47I | GGWCC | 1 cut(s) | 164 |
| EcoRI | GAATTC | 1 cut(s) | 535 |
| FaeI | CATG | 1 cut(s) | 139 |
| FaiI | YATR | 8 cut(s) | 137, 162, 173, 305, 455, 497, 506, 592 |
| FatI | CATG | 1 cut(s) | 135 |
| Fnu4HI | GCNGC | 4 cut(s) | 18, 51, 54, 525 |
| FokI | GGATG | 2 cut(s) | 21, 236 |
| Fsp4HI | GCNGC | 4 cut(s) | 18, 51, 54, 525 |
| GluI | GCNGC | 4 cut(s) | 18, 51, 54, 525 |
| HaeIII | GGCC | 3 cut(s) | 17, 35, 442 |
| HapII | CCGG | 2 cut(s) | 36, 546 |
| Hin1II | CATG | 1 cut(s) | 139 |
| HincII | GTYRAC | 1 cut(s) | 403 |
| HindII | GTYRAC | 1 cut(s) | 403 |
| HindIII | AAGCTT | 2 cut(s) | 212, 482 |
| HinfI | GANTC | 3 cut(s) | 76, 111, 233 |
| HpaI | GTTAAC | 1 cut(s) | 403 |
| HpaII | CCGG | 2 cut(s) | 36, 546 |
| HphI | GGTGA | 2 cut(s) | 237, 292 |
| Hpy166II | GTNNAC | 2 cut(s) | 403, 513 |
| Hpy188I | TCNGA | 3 cut(s) | 99, 110, 297 |
| Hpy188III | TCNNGA | 2 cut(s) | 286, 559 |
| Hpy8I | GTNNAC | 2 cut(s) | 403, 513 |
| HpyAV | CCTTC | 2 cut(s) | 7, 82 |
| HpyCH4III | ACNGT | 2 cut(s) | 334, 472 |
| HpyCH4V | TGCA | 4 cut(s) | 270, 362, 419, 527 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 41, 50, 570, 587 |
| HpyF3I | CTNAG | 1 cut(s) | 458 |
| Hsp92II | CATG | 1 cut(s) | 139 |
| KspAI | GTTAAC | 1 cut(s) | 403 |
| Kzo9I | GATC | 3 cut(s) | 105, 282, 548 |
| LmnI | GCTCC | 1 cut(s) | 298 |
| LpnPI | CCDG | 6 cut(s) | 12, 49, 250, 435, 559, 559 |
| Lsp1109I | GCAGC | 2 cut(s) | 62, 511 |
| LweI | GCATC | 3 cut(s) | 133, 214, 428 |
| MaeIII | GTNAC | 4 cut(s) | 73, 120, 355, 433 |
| MalI | GATC | 3 cut(s) | 107, 284, 550 |
| MboI | GATC | 3 cut(s) | 105, 282, 548 |
| MboII | GAAGA | 2 cut(s) | 164, 209 |
| MhlI | GDGCHC | 1 cut(s) | 179 |
| MluCI | AATT | 5 cut(s) | 80, 272, 408, 535, 609 |
| MlyI | GAGTC | 2 cut(s) | 70, 227 |
| MnlI | CCTC | 5 cut(s) | 57, 223, 271, 354, 562 |
| MseI | TTAA | 2 cut(s) | 402, 613 |
| MspA1I | CMGCKG | 1 cut(s) | 53 |
| MspI | CCGG | 2 cut(s) | 36, 546 |
| MspR9I | CCNGG | 1 cut(s) | 37 |
| Mva1269I | GAATGC | 1 cut(s) | 295 |
| MvnI | CGCG | 1 cut(s) | 20 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 41, 50, 570, 587 |
| NciI | CCSGG | 1 cut(s) | 37 |
| NdeII | GATC | 3 cut(s) | 105, 282, 548 |
| NlaIII | CATG | 1 cut(s) | 139 |
| NlaIV | GGNNCC | 1 cut(s) | 34 |
| NmeAIII | GCCGAG | 2 cut(s) | 253, 293 |
| NmuCI | GTSAC | 2 cut(s) | 73, 120 |
| NspI | RCATGY | 1 cut(s) | 139 |
| PaqCI | CACCTGC | 1 cut(s) | 564 |
| PctI | GAATGC | 1 cut(s) | 295 |
| PfeI | GAWTC | 1 cut(s) | 111 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 449 |
| PkrI | GCNGC | 4 cut(s) | 19, 52, 55, 526 |
| PleI | GAGTC | 2 cut(s) | 70, 227 |
| PpsI | GAGTC | 2 cut(s) | 70, 227 |
| PspN4I | GGNNCC | 1 cut(s) | 34 |
| PspPI | GGNCC | 2 cut(s) | 33, 164 |
| PstI | CTGCAG | 1 cut(s) | 529 |
| SaqAI | TTAA | 2 cut(s) | 402, 613 |
| SatI | GCNGC | 4 cut(s) | 18, 51, 54, 525 |
| Sau3AI | GATC | 3 cut(s) | 105, 282, 548 |
| Sau96I | GGNCC | 2 cut(s) | 33, 164 |
| SchI | GAGTC | 2 cut(s) | 70, 227 |
| ScrFI | CCNGG | 1 cut(s) | 37 |
| SduI | GDGCHC | 1 cut(s) | 179 |
| SetI | ASST | 7 cut(s) | 68, 169, 216, 266, 282, 486, 578 |
| SfaNI | GCATC | 3 cut(s) | 133, 214, 428 |
| SfcI | CTRYAG | 2 cut(s) | 333, 525 |
| SinI | GGWCC | 1 cut(s) | 164 |
| SmlI | CTYRAG | 3 cut(s) | 235, 345, 559 |
| SmoI | CTYRAG | 3 cut(s) | 235, 345, 559 |
| Sse9I | AATT | 5 cut(s) | 80, 272, 408, 535, 609 |
| SsiI | CCGC | 3 cut(s) | 18, 44, 53 |
| StyD4I | CCNGG | 1 cut(s) | 35 |
| TaaI | ACNGT | 2 cut(s) | 334, 472 |
| TaqI | TCGA | 1 cut(s) | 114 |
| TasI | AATT | 5 cut(s) | 80, 272, 408, 535, 609 |
| TauI | GCSGC | 2 cut(s) | 20, 56 |
| TfiI | GAWTC | 1 cut(s) | 111 |
| Tru1I | TTAA | 2 cut(s) | 402, 613 |
| Tru9I | TTAA | 2 cut(s) | 402, 613 |
| TscAI | CASTG | 1 cut(s) | 129 |
| TseFI | GTSAC | 2 cut(s) | 73, 120 |
| TseI | GCWGC | 2 cut(s) | 50, 524 |
| Tsp45I | GTSAC | 2 cut(s) | 73, 120 |
| TspDTI | ATGAA | 1 cut(s) | 224 |
| TspGWI | ACGGA | 2 cut(s) | 404, 467 |
| TspRI | CASTG | 1 cut(s) | 129 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 449 |
| VpaK11BI | GGWCC | 1 cut(s) | 164 |
| XapI | RAATTY | 3 cut(s) | 272, 535, 609 |
| XceI | RCATGY | 1 cut(s) | 139 |