DNA polymerase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 27 |
| AccII | CGCG | 2 cut(s) | 45, 169 |
| AciI | CCGC | 4 cut(s) | 43, 167, 169, 431 |
| AfaI | GTAC | 1 cut(s) | 41 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 78, 129 |
| AflII | CTTAAG | 1 cut(s) | 464 |
| AgsI | TTSAA | 1 cut(s) | 472 |
| AjiI | CACGTC | 1 cut(s) | 246 |
| Alw26I | GTCTC | 1 cut(s) | 352 |
| AoxI | GGCC | 3 cut(s) | 119, 170, 282 |
| ApeKI | GCWGC | 1 cut(s) | 206 |
| AspS9I | GGNCC | 2 cut(s) | 69, 120 |
| AsuHPI | GGTGA | 1 cut(s) | 287 |
| AvaII | GGWCC | 1 cut(s) | 69 |
| BaeGI | GKGCMC | 1 cut(s) | 373 |
| BarI | GAAGNNNNNNTAC | 1 cut(s) | 31 |
| BbsI | GAAGAC | 1 cut(s) | 140 |
| BbvI | GCAGC | 1 cut(s) | 218 |
| BccI | CCATC | 2 cut(s) | 208, 266 |
| BceAI | ACGGC | 2 cut(s) | 67, 213 |
| BcoDI | GTCTC | 1 cut(s) | 352 |
| BfaI | CTAG | 1 cut(s) | 481 |
| BfrI | CTTAAG | 1 cut(s) | 464 |
| BisI | GCNGC | 3 cut(s) | 167, 170, 207 |
| BlsI | GCNGC | 3 cut(s) | 168, 171, 208 |
| Bme18I | GGWCC | 1 cut(s) | 69 |
| BmgBI | CACGTC | 1 cut(s) | 246 |
| BmgT120I | GGNCC | 2 cut(s) | 69, 120 |
| BmiI | GGNNCC | 2 cut(s) | 71, 165 |
| BpiI | GAAGAC | 1 cut(s) | 140 |
| BsaJI | CCNNGG | 1 cut(s) | 167 |
| BsaWI | WCCGGW | 1 cut(s) | 401 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 78, 129 |
| Bse118I | RCCGGY | 1 cut(s) | 376 |
| Bse1I | ACTGG | 1 cut(s) | 71 |
| BseDI | CCNNGG | 1 cut(s) | 167 |
| BseGI | GGATG | 2 cut(s) | 103, 277 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 78, 129 |
| BseNI | ACTGG | 1 cut(s) | 71 |
| BseRI | GAGGAG | 3 cut(s) | 142, 379, 397 |
| BseSI | GKGCMC | 1 cut(s) | 373 |
| BseXI | GCAGC | 1 cut(s) | 218 |
| Bsh1236I | CGCG | 2 cut(s) | 45, 169 |
| Bsh1285I | CGRYCG | 1 cut(s) | 27 |
| BshFI | GGCC | 3 cut(s) | 121, 172, 284 |
| BsiEI | CGRYCG | 1 cut(s) | 27 |
| BsiSI | CCGG | 2 cut(s) | 377, 402 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 78, 129 |
| BsmAI | GTCTC | 1 cut(s) | 352 |
| BsmBI | CGTCTC | 1 cut(s) | 352 |
| BsnI | GGCC | 3 cut(s) | 121, 172, 284 |
| Bsp1286I | GDGCHC | 1 cut(s) | 373 |
| Bsp143I | GATC | 1 cut(s) | 12 |
| BspACI | CCGC | 4 cut(s) | 43, 167, 169, 431 |
| BspANI | GGCC | 3 cut(s) | 121, 172, 284 |
| BspFNI | CGCG | 2 cut(s) | 45, 169 |
| BspLI | GGNNCC | 2 cut(s) | 71, 165 |
| BspTI | CTTAAG | 1 cut(s) | 464 |
| BsrFI | RCCGGY | 1 cut(s) | 376 |
| BsrI | ACTGG | 1 cut(s) | 71 |
| BssAI | RCCGGY | 1 cut(s) | 376 |
| BssECI | CCNNGG | 1 cut(s) | 167 |
| BssMI | GATC | 1 cut(s) | 12 |
| Bst4CI | ACNGT | 2 cut(s) | 25, 139 |
| Bst6I | CTCTTC | 1 cut(s) | 30 |
| BstAFI | CTTAAG | 1 cut(s) | 464 |
| BstC8I | GCNNGC | 1 cut(s) | 378 |
| BstDSI | CCRYGG | 1 cut(s) | 167 |
| BstF5I | GGATG | 2 cut(s) | 103, 277 |
| BstFNI | CGCG | 2 cut(s) | 45, 169 |
| BstKTI | GATC | 1 cut(s) | 15 |
| BstMAI | GTCTC | 1 cut(s) | 352 |
| BstMBI | GATC | 1 cut(s) | 12 |
| BstMCI | CGRYCG | 1 cut(s) | 27 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 51, 178, 206, 377 |
| BstNSI | RCATGY | 1 cut(s) | 440 |
| BstSLI | GKGCMC | 1 cut(s) | 373 |
| BstUI | CGCG | 2 cut(s) | 45, 169 |
| BstV1I | GCAGC | 1 cut(s) | 218 |
| BstV2I | GAAGAC | 1 cut(s) | 140 |
| BsuRI | GGCC | 3 cut(s) | 121, 172, 284 |
| BtgI | CCRYGG | 1 cut(s) | 167 |
| BtgZI | GCGATG | 1 cut(s) | 224 |
| BtrI | CACGTC | 1 cut(s) | 246 |
| BtsCI | GGATG | 2 cut(s) | 103, 277 |
| Cac8I | GCNNGC | 1 cut(s) | 378 |
| Cfr10I | RCCGGY | 1 cut(s) | 376 |
| Cfr13I | GGNCC | 2 cut(s) | 69, 120 |
| Cfr42I | CCGCGG | 1 cut(s) | 170 |
| Csp6I | GTAC | 1 cut(s) | 40 |
| CviAII | CATG | 1 cut(s) | 437 |
| CviQI | GTAC | 1 cut(s) | 40 |
| DpnI | GATC | 1 cut(s) | 14 |
| DpnII | GATC | 1 cut(s) | 12 |
| Eam1104I | CTCTTC | 1 cut(s) | 30 |
| EarI | CTCTTC | 1 cut(s) | 30 |
| Eco47I | GGWCC | 1 cut(s) | 69 |
| Esp3I | CGTCTC | 1 cut(s) | 352 |
| FaeI | CATG | 1 cut(s) | 440 |
| FaiI | YATR | 4 cut(s) | 116, 315, 438, 459 |
| FatI | CATG | 1 cut(s) | 436 |
| FblI | GTMKAC | 1 cut(s) | 27 |
| Fnu4HI | GCNGC | 3 cut(s) | 167, 170, 207 |
| FokI | GGATG | 2 cut(s) | 110, 284 |
| Fsp4HI | GCNGC | 3 cut(s) | 167, 170, 207 |
| FspBI | CTAG | 1 cut(s) | 481 |
| GluI | GCNGC | 3 cut(s) | 167, 170, 207 |
| HaeIII | GGCC | 3 cut(s) | 121, 172, 284 |
| HapII | CCGG | 2 cut(s) | 377, 402 |
| Hin1II | CATG | 1 cut(s) | 440 |
| HincII | GTYRAC | 1 cut(s) | 28 |
| HindII | GTYRAC | 1 cut(s) | 28 |
| HinfI | GANTC | 1 cut(s) | 101 |
| HpaII | CCGG | 2 cut(s) | 377, 402 |
| HphI | GGTGA | 1 cut(s) | 287 |
| Hpy166II | GTNNAC | 2 cut(s) | 28, 243 |
| Hpy188I | TCNGA | 3 cut(s) | 87, 97, 106 |
| Hpy8I | GTNNAC | 2 cut(s) | 28, 243 |
| Hpy99I | CGWCG | 7 cut(s) | 32, 50, 53, 78, 97, 193, 238 |
| HpyAV | CCTTC | 2 cut(s) | 142, 154 |
| HpyCH4III | ACNGT | 2 cut(s) | 25, 139 |
| HpyCH4IV | ACGT | 1 cut(s) | 245 |
| HpyCH4V | TGCA | 1 cut(s) | 436 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 51, 178, 206, 377 |
| HpySE526I | ACGT | 1 cut(s) | 245 |
| Hsp92II | CATG | 1 cut(s) | 440 |
| KroI | GCCGGC | 1 cut(s) | 376 |
| KroNI | GCCGGC | 1 cut(s) | 378 |
| KspI | CCGCGG | 1 cut(s) | 170 |
| Kzo9I | GATC | 1 cut(s) | 12 |
| LmnI | GCTCC | 1 cut(s) | 163 |
| LpnPI | CCDG | 4 cut(s) | 52, 351, 390, 415 |
| Lsp1109I | GCAGC | 1 cut(s) | 218 |
| MaeI | CTAG | 1 cut(s) | 481 |
| MaeII | ACGT | 1 cut(s) | 245 |
| MaeIII | GTNAC | 1 cut(s) | 275 |
| MalI | GATC | 1 cut(s) | 14 |
| MboI | GATC | 1 cut(s) | 12 |
| MboII | GAAGA | 3 cut(s) | 47, 145, 157 |
| MhlI | GDGCHC | 1 cut(s) | 373 |
| MluCI | AATT | 1 cut(s) | 184 |
| MmeI | TCCRAC | 1 cut(s) | 75 |
| MroNI | GCCGGC | 1 cut(s) | 376 |
| MseI | TTAA | 3 cut(s) | 258, 408, 465 |
| MslI | CAYNNNNRTG | 1 cut(s) | 441 |
| MspA1I | CMGCKG | 1 cut(s) | 169 |
| MspCI | CTTAAG | 1 cut(s) | 464 |
| MspI | CCGG | 2 cut(s) | 377, 402 |
| MvnI | CGCG | 2 cut(s) | 45, 169 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 51, 178, 206, 377 |
| NaeI | GCCGGC | 1 cut(s) | 378 |
| NdeII | GATC | 1 cut(s) | 12 |
| NgoMIV | GCCGGC | 1 cut(s) | 376 |
| NlaIII | CATG | 1 cut(s) | 440 |
| NlaIV | GGNNCC | 2 cut(s) | 71, 165 |
| NmeAIII | GCCGAG | 1 cut(s) | 352 |
| NmuCI | GTSAC | 1 cut(s) | 275 |
| NspI | RCATGY | 1 cut(s) | 440 |
| PcsI | WCGNNNNNNNCGW | 3 cut(s) | 24, 197, 242 |
| PdiI | GCCGGC | 1 cut(s) | 378 |
| PfeI | GAWTC | 1 cut(s) | 101 |
| PkrI | GCNGC | 3 cut(s) | 168, 171, 208 |
| PspN4I | GGNNCC | 2 cut(s) | 71, 165 |
| PspPI | GGNCC | 2 cut(s) | 69, 120 |
| RsaI | GTAC | 1 cut(s) | 41 |
| RsaNI | GTAC | 1 cut(s) | 40 |
| RseI | CAYNNNNRTG | 1 cut(s) | 441 |
| SacII | CCGCGG | 1 cut(s) | 170 |
| SalI | GTCGAC | 1 cut(s) | 26 |
| SaqAI | TTAA | 3 cut(s) | 258, 408, 465 |
| SatI | GCNGC | 3 cut(s) | 167, 170, 207 |
| Sau3AI | GATC | 1 cut(s) | 12 |
| Sau96I | GGNCC | 2 cut(s) | 69, 120 |
| SduI | GDGCHC | 1 cut(s) | 373 |
| SetI | ASST | 2 cut(s) | 60, 248 |
| Sfr303I | CCGCGG | 1 cut(s) | 170 |
| SgrBI | CCGCGG | 1 cut(s) | 170 |
| SinI | GGWCC | 1 cut(s) | 69 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 441 |
| SmlI | CTYRAG | 1 cut(s) | 464 |
| SmoI | CTYRAG | 1 cut(s) | 464 |
| Sse9I | AATT | 1 cut(s) | 184 |
| SsiI | CCGC | 4 cut(s) | 43, 167, 169, 431 |
| SspMI | CTAG | 1 cut(s) | 481 |
| TaaI | ACNGT | 2 cut(s) | 25, 139 |
| TaiI | ACGT | 1 cut(s) | 248 |
| TaqI | TCGA | 4 cut(s) | 27, 236, 395, 441 |
| TasI | AATT | 1 cut(s) | 184 |
| TauI | GCSGC | 2 cut(s) | 169, 172 |
| TfiI | GAWTC | 1 cut(s) | 101 |
| Tru1I | TTAA | 3 cut(s) | 258, 408, 465 |
| Tru9I | TTAA | 3 cut(s) | 258, 408, 465 |
| TseFI | GTSAC | 1 cut(s) | 275 |
| TseI | GCWGC | 1 cut(s) | 206 |
| Tsp45I | GTSAC | 1 cut(s) | 275 |
| TspDTI | ATGAA | 1 cut(s) | 114 |
| TspGWI | ACGGA | 1 cut(s) | 376 |
| Vha464I | CTTAAG | 1 cut(s) | 464 |
| VpaK11BI | GGWCC | 1 cut(s) | 69 |
| XceI | RCATGY | 1 cut(s) | 440 |
| XmiI | GTMKAC | 1 cut(s) | 27 |
| XspI | CTAG | 1 cut(s) | 481 |