DNA polymerase
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 487 |
| AccB1I | GGYRCC | 1 cut(s) | 436 |
| AccII | CGCG | 1 cut(s) | 98 |
| AciI | CCGC | 3 cut(s) | 96, 132, 368 |
| AclWI | GGATC | 1 cut(s) | 417 |
| AcuI | CTGAAG | 2 cut(s) | 357, 521 |
| AfaI | GTAC | 3 cut(s) | 29, 498, 598 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 254, 521 |
| AgsI | TTSAA | 1 cut(s) | 308 |
| AluBI | AGCT | 4 cut(s) | 304, 358, 425, 556 |
| AluI | AGCT | 4 cut(s) | 304, 358, 425, 556 |
| AlwI | GGATC | 1 cut(s) | 417 |
| AoxI | GGCC | 2 cut(s) | 93, 111 |
| ApeKI | GCWGC | 2 cut(s) | 129, 358 |
| AspS9I | GGNCC | 2 cut(s) | 111, 245 |
| AsuC2I | CCSGG | 2 cut(s) | 115, 521 |
| AsuHPI | GGTGA | 1 cut(s) | 327 |
| AvaII | GGWCC | 1 cut(s) | 245 |
| BaeGI | GKGCMC | 1 cut(s) | 260 |
| BanI | GGYRCC | 1 cut(s) | 436 |
| BbvI | GCAGC | 2 cut(s) | 141, 345 |
| BccI | CCATC | 2 cut(s) | 81, 191 |
| BcnI | CCSGG | 2 cut(s) | 115, 521 |
| BfmI | CTRYAG | 2 cut(s) | 480, 571 |
| BfuAI | ACCTGC | 1 cut(s) | 487 |
| BisI | GCNGC | 5 cut(s) | 96, 130, 133, 359, 369 |
| BlsI | GCNGC | 5 cut(s) | 97, 131, 134, 360, 370 |
| BmcAI | AGTACT | 1 cut(s) | 598 |
| Bme1390I | CCNGG | 2 cut(s) | 115, 521 |
| Bme18I | GGWCC | 1 cut(s) | 245 |
| BmgT120I | GGNCC | 2 cut(s) | 111, 245 |
| BmiI | GGNNCC | 2 cut(s) | 112, 438 |
| BmrFI | CCNGG | 2 cut(s) | 115, 521 |
| BmrI | ACTGGG | 1 cut(s) | 38 |
| BmsI | GCATC | 3 cut(s) | 214, 304, 355 |
| BmuI | ACTGGG | 1 cut(s) | 38 |
| BpuEI | CTTGAG | 2 cut(s) | 310, 482 |
| BpuMI | CCSGG | 2 cut(s) | 115, 521 |
| BsaBI | GATNNNNATC | 1 cut(s) | 183 |
| BsaJI | CCNNGG | 2 cut(s) | 318, 520 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 254, 521 |
| Bse1I | ACTGG | 3 cut(s) | 44, 109, 127 |
| Bse3DI | GCAATG | 1 cut(s) | 142 |
| Bse8I | GATNNNNATC | 1 cut(s) | 183 |
| BseDI | CCNNGG | 2 cut(s) | 318, 520 |
| BseGI | GGATG | 3 cut(s) | 79, 92, 319 |
| BseJI | GATNNNNATC | 1 cut(s) | 183 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 254, 521 |
| BseMI | GCAATG | 1 cut(s) | 142 |
| BseNI | ACTGG | 3 cut(s) | 44, 109, 127 |
| BseSI | GKGCMC | 1 cut(s) | 260 |
| BseXI | GCAGC | 2 cut(s) | 141, 345 |
| Bsh1236I | CGCG | 1 cut(s) | 98 |
| BshFI | GGCC | 2 cut(s) | 95, 113 |
| BshNI | GGYRCC | 1 cut(s) | 436 |
| BsiSI | CCGG | 2 cut(s) | 114, 520 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 254, 521 |
| BsnI | GGCC | 2 cut(s) | 95, 113 |
| Bsp1286I | GDGCHC | 1 cut(s) | 260 |
| Bsp143I | GATC | 4 cut(s) | 184, 409, 467, 504 |
| BspACI | CCGC | 3 cut(s) | 96, 132, 368 |
| BspANI | GGCC | 2 cut(s) | 95, 113 |
| BspFNI | CGCG | 1 cut(s) | 98 |
| BspLI | GGNNCC | 2 cut(s) | 112, 438 |
| BspMAI | CTGCAG | 1 cut(s) | 484 |
| BspMI | ACCTGC | 1 cut(s) | 487 |
| BspPI | GGATC | 1 cut(s) | 417 |
| BspT107I | GGYRCC | 1 cut(s) | 436 |
| BsrDI | GCAATG | 1 cut(s) | 142 |
| BsrI | ACTGG | 3 cut(s) | 44, 109, 127 |
| BssECI | CCNNGG | 2 cut(s) | 318, 520 |
| BssMI | GATC | 4 cut(s) | 184, 409, 467, 504 |
| Bst4CI | ACNGT | 2 cut(s) | 262, 592 |
| Bst6I | CTCTTC | 1 cut(s) | 228 |
| BstF5I | GGATG | 3 cut(s) | 79, 92, 319 |
| BstFNI | CGCG | 1 cut(s) | 98 |
| BstKTI | GATC | 4 cut(s) | 187, 412, 470, 507 |
| BstMBI | GATC | 4 cut(s) | 184, 409, 467, 504 |
| BstNSI | RCATGY | 1 cut(s) | 220 |
| BstSCI | CCNGG | 2 cut(s) | 113, 519 |
| BstSFI | CTRYAG | 2 cut(s) | 480, 571 |
| BstSLI | GKGCMC | 1 cut(s) | 260 |
| BstUI | CGCG | 1 cut(s) | 98 |
| BstV1I | GCAGC | 2 cut(s) | 141, 345 |
| BstX2I | RGATCY | 1 cut(s) | 409 |
| BstYI | RGATCY | 1 cut(s) | 409 |
| BsuRI | GGCC | 2 cut(s) | 95, 113 |
| BtgZI | GCGATG | 2 cut(s) | 113, 597 |
| BtsCI | GGATG | 3 cut(s) | 79, 92, 319 |
| BtsI | GCAGTG | 2 cut(s) | 66, 203 |
| BtsIMutI | CAGTG | 3 cut(s) | 66, 203, 258 |
| BveI | ACCTGC | 1 cut(s) | 487 |
| Cfr13I | GGNCC | 2 cut(s) | 111, 245 |
| Csp6I | GTAC | 3 cut(s) | 28, 497, 597 |
| CviAII | CATG | 2 cut(s) | 217, 362 |
| CviJI | RGCY | 8 cut(s) | 95, 113, 118, 304, 358, 371, 425, 556 |
| CviKI_1 | RGCY | 8 cut(s) | 95, 113, 118, 304, 358, 371, 425, 556 |
| CviQI | GTAC | 3 cut(s) | 28, 497, 597 |
| DpnI | GATC | 4 cut(s) | 186, 411, 469, 506 |
| DpnII | GATC | 4 cut(s) | 184, 409, 467, 504 |
| Eam1104I | CTCTTC | 1 cut(s) | 228 |
| EarI | CTCTTC | 1 cut(s) | 228 |
| Eco47I | GGWCC | 1 cut(s) | 245 |
| Eco57I | CTGAAG | 2 cut(s) | 357, 521 |
| FaeI | CATG | 2 cut(s) | 220, 365 |
| FatI | CATG | 2 cut(s) | 216, 361 |
| FauNDI | CATATG | 1 cut(s) | 32 |
| Fnu4HI | GCNGC | 5 cut(s) | 96, 130, 133, 359, 369 |
| FokI | GGATG | 3 cut(s) | 86, 99, 326 |
| Fsp4HI | GCNGC | 5 cut(s) | 96, 130, 133, 359, 369 |
| GluI | GCNGC | 5 cut(s) | 96, 130, 133, 359, 369 |
| HaeIII | GGCC | 2 cut(s) | 95, 113 |
| HapII | CCGG | 2 cut(s) | 114, 520 |
| Hin1II | CATG | 2 cut(s) | 220, 365 |
| HinfI | GANTC | 3 cut(s) | 155, 191, 323 |
| HpaII | CCGG | 2 cut(s) | 114, 520 |
| HphI | GGTGA | 1 cut(s) | 327 |
| Hpy188I | TCNGA | 4 cut(s) | 178, 196, 376, 472 |
| Hpy188III | TCNNGA | 3 cut(s) | 188, 445, 461 |
| HpyAV | CCTTC | 3 cut(s) | 85, 161, 271 |
| HpyCH4III | ACNGT | 2 cut(s) | 262, 592 |
| HpyCH4IV | ACGT | 1 cut(s) | 541 |
| HpyCH4V | TGCA | 2 cut(s) | 361, 482 |
| HpySE526I | ACGT | 1 cut(s) | 541 |
| Hsp92II | CATG | 2 cut(s) | 220, 365 |
| Kzo9I | GATC | 4 cut(s) | 184, 409, 467, 504 |
| Lsp1109I | GCAGC | 2 cut(s) | 141, 345 |
| LweI | GCATC | 3 cut(s) | 214, 304, 355 |
| MaeII | ACGT | 1 cut(s) | 541 |
| MaeIII | GTNAC | 4 cut(s) | 152, 201, 542, 608 |
| MalI | GATC | 4 cut(s) | 186, 411, 469, 506 |
| MboI | GATC | 4 cut(s) | 184, 409, 467, 504 |
| MboII | GAAGA | 2 cut(s) | 245, 514 |
| MflI | RGATCY | 1 cut(s) | 409 |
| MhlI | GDGCHC | 1 cut(s) | 260 |
| MluCI | AATT | 7 cut(s) | 20, 159, 377, 389, 490, 508, 625 |
| MlyI | GAGTC | 2 cut(s) | 149, 317 |
| MmeI | TCCRAC | 1 cut(s) | 387 |
| MnlI | CCTC | 4 cut(s) | 136, 313, 441, 572 |
| MseI | TTAA | 4 cut(s) | 39, 51, 282, 380 |
| MspA1I | CMGCKG | 1 cut(s) | 132 |
| MspI | CCGG | 2 cut(s) | 114, 520 |
| MspR9I | CCNGG | 2 cut(s) | 115, 521 |
| MvnI | CGCG | 1 cut(s) | 98 |
| NciI | CCSGG | 2 cut(s) | 115, 521 |
| NdeI | CATATG | 1 cut(s) | 32 |
| NdeII | GATC | 4 cut(s) | 184, 409, 467, 504 |
| NlaIII | CATG | 2 cut(s) | 220, 365 |
| NlaIV | GGNNCC | 2 cut(s) | 112, 438 |
| NmeAIII | GCCGAG | 1 cut(s) | 343 |
| NmuCI | GTSAC | 3 cut(s) | 152, 201, 542 |
| NspI | RCATGY | 1 cut(s) | 220 |
| PfeI | GAWTC | 1 cut(s) | 191 |
| PkrI | GCNGC | 5 cut(s) | 97, 131, 134, 360, 370 |
| PleI | GAGTC | 2 cut(s) | 149, 317 |
| PpsI | GAGTC | 2 cut(s) | 149, 317 |
| PspN4I | GGNNCC | 2 cut(s) | 112, 438 |
| PspPI | GGNCC | 2 cut(s) | 111, 245 |
| PstI | CTGCAG | 1 cut(s) | 484 |
| PsuI | RGATCY | 1 cut(s) | 409 |
| RsaI | GTAC | 3 cut(s) | 29, 498, 598 |
| RsaNI | GTAC | 3 cut(s) | 28, 497, 597 |
| SaqAI | TTAA | 4 cut(s) | 39, 51, 282, 380 |
| SatI | GCNGC | 5 cut(s) | 96, 130, 133, 359, 369 |
| Sau3AI | GATC | 4 cut(s) | 184, 409, 467, 504 |
| Sau96I | GGNCC | 2 cut(s) | 111, 245 |
| SbfI | CCTGCAGG | 1 cut(s) | 484 |
| ScaI | AGTACT | 1 cut(s) | 598 |
| SchI | GAGTC | 2 cut(s) | 149, 317 |
| ScrFI | CCNGG | 2 cut(s) | 115, 521 |
| SdaI | CCTGCAGG | 1 cut(s) | 484 |
| SduI | GDGCHC | 1 cut(s) | 260 |
| SfaNI | GCATC | 3 cut(s) | 214, 304, 355 |
| SfcI | CTRYAG | 2 cut(s) | 480, 571 |
| SinI | GGWCC | 1 cut(s) | 245 |
| SmlI | CTYRAG | 2 cut(s) | 325, 461 |
| SmoI | CTYRAG | 2 cut(s) | 325, 461 |
| Sse8387I | CCTGCAGG | 1 cut(s) | 484 |
| Sse9I | AATT | 7 cut(s) | 20, 159, 377, 389, 490, 508, 625 |
| SsiI | CCGC | 3 cut(s) | 96, 132, 368 |
| StyD4I | CCNGG | 2 cut(s) | 113, 519 |
| TaaI | ACNGT | 2 cut(s) | 262, 592 |
| TaiI | ACGT | 1 cut(s) | 544 |
| TasI | AATT | 7 cut(s) | 20, 159, 377, 389, 490, 508, 625 |
| TatI | WGTACW | 2 cut(s) | 496, 596 |
| TauI | GCSGC | 3 cut(s) | 98, 135, 371 |
| TfiI | GAWTC | 1 cut(s) | 191 |
| Tru1I | TTAA | 4 cut(s) | 39, 51, 282, 380 |
| Tru9I | TTAA | 4 cut(s) | 39, 51, 282, 380 |
| TscAI | CASTG | 3 cut(s) | 66, 210, 265 |
| TseFI | GTSAC | 3 cut(s) | 152, 201, 542 |
| TseI | GCWGC | 2 cut(s) | 129, 358 |
| Tsp45I | GTSAC | 3 cut(s) | 152, 201, 542 |
| TspDTI | ATGAA | 3 cut(s) | 285, 402, 600 |
| TspRI | CASTG | 3 cut(s) | 66, 210, 265 |
| VpaK11BI | GGWCC | 1 cut(s) | 245 |
| XceI | RCATGY | 1 cut(s) | 220 |
| ZrmI | AGTACT | 1 cut(s) | 598 |